Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| comparison of respiratory burst activity of inflammatory neutrophils in ayu (plecoglossus altivelis) and carp (cyprinus carpio). | neutrophils of ayu (plecoglossus altivelis) were previously shown to have unusually high respiratory burst activity (rba). to understand this unique character of ayu neutrophils, the rbas of resting and inflammatory neutrophils of ayu and carp (cyprinus carpio) were compared. inflammation was induced in the peritoneal cavity by injecting killed-bacteria. the rba of peritoneal-exudate (inflammatory) neutrophils was measured after stimulation with phorbol myristate acetate (pma). resting neutrophi ... | 2005 | 15863016 |
| administration of yeast glucan enhances survival and some non-specific and specific immune parameters in carp (cyprinus carpio) infected with aeromonas hydrophila. | effects of beta-glucan administration on survival and immune modulations were studied in cyprinus carpio against the bacterial pathogen, aeromonas hydrophila. beta-glucan was extracted from saccharomyces cervisiae and purified. a virulent strain of the pathogen a. hydrophila was collected from infected fish. different concentrations of beta-glucan were administered to test animals on day 1, 3 and 5 through different routes (intraperitoneal injection (ip), bathing and oral administration). contro ... | 2005 | 15863011 |
| quantitative structure-activity relationships for the toxicity of substituted benzenes to cyprinus carpio. | to measure the 96h-lc50 values of 32 substituted benzenes to the carp and to study the relationship between quantitative structure-activity and structural parameters of chemicals. | 2005 | 15861779 |
| molecular cloning and characterization of two types of cd8alpha from ginbuna crucian carp, carassius auratus langsdorfii. | we cloned and sequenced full-length cdnas for two types of cd8alpha from the s3n strain of ginbuna crucian carp (carassius auratus langsdorfii) and quantified the expression of cd8alpha genes after sensitization by scale grafting, employing a model system of clonal triploid ginbuna and tetraploid ginbuna-goldfish hybrids. rt-pcr yielded four different fragments of cd8alpha homologue from the s3n strain of ginbuna and these sequences were classified into two groups. the two types of ginbuna cd8al ... | 2005 | 15854681 |
| gill lesions in the major carp, labeo rohita exposed to lethal and sublethal concentrations of tannery effluent. | the major carp, labeo rohita were exposed to (0.873%) lethal and sublethal (0.073%) concentrations of tannery effluent for 24h and 40 days respectively under static bioassay condition. the surface architecture of gill revealed severe damages such as : fusion and clumping in the middle and distal parts of the primary lamellae, swelling and deterioration of the cells. the interlamellar space was filled either with hyperplastic epithelial or mucous cells. secondary lamellae lost their identity and ... | 2004 | 15847345 |
| first ovarian maturity and recovery response in common carp, cyprinus carpio after exposure to carbofuran. | the present study embodies the observations on duration (45, 75 and 105 days) dependent effects of toxicologically low concentration (16 ppm) of a carbamate pesticide, carbofuran, on first ovarian maturity with correlative recovery response by withdrawal of treatment for 30 days in virgin yearlings of common carp, cyprinus carpio var. communis. in control fish histological organization of ovary and changes in gonado-somatic index (gsi) revealed the initiation of oocyte differentiation and develo ... | 2004 | 15847330 |
| [construction and characteristics analysis of somatic cell lines from common carp cyprinus carpio]. | epithelia of rostral side and fins from common carp were taken to culture in vitro. as a result, cells of tail fins were successfully passed from generation to generation, and constructed cell lines. during the culture procedure, the passage cells were observed and their biological characteristics were analyzed, which consist of morphologic analysis, drawing of growth curve, detection of producing rate for clones, insight of adaptation to temperatures, calculation of chromosomes and investigatio ... | 2005 | 15839211 |
| protection of cultured cyprinus carpio against a lethal viral disease by an attenuated virus vaccine. | massive mortality of koi and common carp--cyprinus carpio species--has been observed since 1998 in many countries worldwide, resulting in severe economic losses. the cause of the disease is an as yet unclassified large dna virus, designated carp nephritis gill necrosis virus (cngv) or koi herpes virus (khv). previously, we demonstrated that the wild type cngv lost its pathogenecity following serial transfer in cell culture, and that clones isolated from the attenuated population can be used as a ... | 2005 | 15837363 |
| importance of photoperiods in the regulation of ovarian activities in indian major carp catla catla in an annual cycle. | the present study attempted for the first time to explore the importance of photoperiod in the regulation of seasonal ovarian functions in any subtropical major carp. adult indian major carp catla catla were transferred to a long photoperiod (lp; ld 16:8) or a short photoperiod (sp; ld 8:16) for 30 days on 4 dates corresponding to the beginnings of 4 reproductive phases in an annual cycle, and responsiveness of the ovary was evaluated by comparison with the gonadal weight (i(g)), relative number ... | 2005 | 15834111 |
| plasma vitellogenin levels and gonadal morphology of wild carp (cyprinus carpio l.) in a receiving rivers downstream of sewage treatment plants. | a field study was carried out in order to assess, in a wild cyprinid fish, the biological effects related to the exposure to estrogenic compounds. the gonadosomic index (gsi), plasma vitellogenin (vtg) level and histological observations of gonads were performed to evaluate the reproductive health in common carp (cyprinus carpio) from three rivers receiving sewage treatment plant (stp) effluents (guadarrama, henares and jarama rivers, spain) and from a reference site (lozoya river). no significa ... | 2004 | 15833242 |
| involvement of gadd153 and cardiac ankyrin repeat protein in hypoxia-induced apoptosis of h9c2 cells. | oxidative stress is the main cause of cardiac injury during ischemia/reperfusion but the molecular mechanism for this process is unclear. in this study, it was found that hypoxia induces apoptosis in rat embryonic heart-derived h9c2 cells leading to the induction of gadd153, which is an apoptosis-related gene. therefore, this study addressed the molecular role of gadd153 in hypoxia-induced apoptosis. the stable or inducible overexpression of gadd153 sensitized the h9c2 cells to apoptotic cell de ... | 2005 | 15826945 |
| caspase-dependent processing activates the proapoptotic activity of deleted in breast cancer-1 during tumor necrosis factor-alpha-mediated death signaling. | deleted in breast cancer-1 (dbc-1) was initially cloned from a homozygously deleted region in breast and other cancers on human chromosome 8p21, although no function is known for the protein product it encodes. we identified the generation of amino-terminally truncated versions of dbc-1 during tumor necrosis factor (tnf)-alpha-mediated apoptosis. full-length 150 kda dbc-1 underwent caspase-dependent processing during tnf-alpha-mediated death signaling, to produce p120 dbc-1 and p66 dbc-1 carboxy ... | 2005 | 15824730 |
| characterisation of t cell antigen receptor alpha chain isotypes in the common carp. | t cell receptor alpha (tcralpha) chain has been characterised in several teleost species to date. here, a reverse transcription-polymerase chain reaction (rt-pcr) strategy was used to isolate cdna clones encoding tcralpha chain from an individual of the common carp (cyprinus carpio.l.). the valpha sequences identified were most similar to valpha of other teleosts, and could be classified into as many as 14 valpha families. for the jalpha sequences, diversity comparable to that seen in other tele ... | 2005 | 15820122 |
| the immunomodulatory effects of the dimer of lysozyme (klp-602) in carp (cyprinus carpio l)--in vivo study. | the dimer of lysozyme was administered to carp (cyprinus carpio l.) in an attempt to reduce the suppression of response in fish exposed to the cypermethrin. experimental studies were carried out on cells isolated from blood and pronephros of carp. in the in vivo studies fish were intoxicated with cypermethrin (synthetic pesticide) at a dose of ld5. after 24 h fish were injected intraperitoneally with a modulator-the dimer of lysozyme (klp-602) at a dose of 0.02 mg/kg of body weight. in the study ... | 2005 | 15814318 |
| expression profiling with progression of dystrophic change in dysferlin-deficient mice (sjl). | the sjl mouse is a model for human dysferlinopathy (limb-girdle muscular dystrophy type 2b and miyoshi myopathy). we used cdna microarrays to compare the expression profiles of 10,012 genes in control and sjl quadriceps femoris muscles in order to find genes involved in the degeneration and regeneration process and in dysferlin's functional network. many genes involved in the process of muscle regeneration are observed to be up-regulated in sjl mice, including cardiac ankyrin repeated protein (c ... | 2005 | 15811552 |
| identification of the genes involved in enhanced fenretinide-induced apoptosis by parthenolide in human hepatoma cells. | fenretinide (n-4-hydroxyphenyl retinamide, 4hpr) is a synthetic anticancer retinoid that is a well-known apoptosis-inducing agent. recently, we observed that the apoptosis induced by fenretinide could be effectively enhanced in hepatoma cells by a concomitant treatment with parthenolide, which is a known inhibitor of nuclear factor-kappab (nf-kappab). furthermore, treatment with fenretinide triggered the activation of nf-kappab during apoptosis, which could be substantially inhibited by partheno ... | 2005 | 15805281 |
| disruption of the gene euchromatin histone methyl transferase1 (eu-hmtase1) is associated with the 9q34 subtelomeric deletion syndrome. | a new syndrome has been recognised following thorough analysis of patients with a terminal submicroscopic subtelomeric deletion of chromosome 9q. these have in common severe mental retardation, hypotonia, brachycephaly, flat face with hypertelorism, synophrys, anteverted nares, thickened lower lip, carp mouth with macroglossia, and conotruncal heart defects. the minimum critical region responsible for this 9q subtelomeric deletion syndrome (9q-) is approximately 1.2 mb and encompasses at least 1 ... | 2005 | 15805155 |
| calcium-permeable ampa receptor plasticity is mediated by subunit-specific interactions with pick1 and nsf. | a recently described form of synaptic plasticity results in dynamic changes in the calcium permeability of synaptic ampa receptors. since the ampa receptor glur2 subunit confers calcium permeability, this plasticity is thought to occur through the dynamic exchange of synaptic glur2-lacking and glur2-containing receptors. to investigate the molecular mechanisms underlying this calcium-permeable ampa receptor plasticity (carp), we examined whether ampa receptor exchange was mediated by subunit-spe ... | 2005 | 15797551 |
| a survey of potential stressor-induced physiological changes in carp (cyprinus carpio) and barbel (barbus bocagei) along the tajo river. | the objective of this study was to evaluate fish response to acute stress induced by confinement after capture. because of the previously reported presence of chemical compounds in the tajo river basin where the study samplinig took place, an exposure biomarker to organic chemicals (retinol) was used. cortisol and glucose were used as stress biomarkers. plasma levels of cholesterol were used as lipidic metabolism indicators, and retinol level was used as a specific exposure biomarker. a referenc ... | 2005 | 15793831 |
| lessons from trail-resistance mechanisms in colorectal cancer cells: paving the road to patient-tailored therapy. | colorectal cancer is one of the leading causes of cancer-related deaths worldwide. intrinsic, as well as acquired, resistance to chemotherapy remains a major problem in the treatment of this disease. it is, therefore, of great importance to develop new, patient-tailored, treatment strategies for colorectal cancer patients. tumor necrosis factor-related apoptosis-inducing ligand (trail) acts through the pro-apoptotic dr4 and dr5 receptors in tumor cells without harming normal cells and will soon ... | 2004 | 15790545 |
| uptake and enantioselective elimination of chlordane compounds by common carp (cyprinus carpio, l.). | an analytical method involving supercritical fluid extraction (sfe) followed by a two-dimensional gas chromatography (2d-gc) analysis was developed to determine the concentration (first gc) and enantiomeric ratio (second gc) of cis- and trans-chlordanes at the ppb (ng/g) level in fish tissue. the sfe method allowed concentration of the compounds of interest, and reduced the number of extraction and sample clean-up manipulations as compared to classical solvent extraction techniques. four hundred ... | 2005 | 15788172 |
| undetectable apolipoprotein a-i gene expression suggests an unusual mechanism of dietary lipid mobilisation in the intestine of cyprinus carpio. | high density lipoprotein (hdl) has been shown to play an important role in the dietary lipid mobilisation in the carp. in spite of this, previous studies have failed to demonstrate the synthesis of the major protein component of hdl, apolipoprotein a-i (apoa-i), in the proximal intestine of the carp. therefore, the aim of the present study was to evaluate the expression of apoa-i throughout the entire intestine. curiously, no transcription of the apoa-i gene could be detected either by northern ... | 2005 | 15781899 |
| hypoxia and recovery perturb free radical processes and antioxidant potential in common carp (cyprinus carpio) tissues. | the effects of hypoxia exposure and subsequent normoxic recovery on the levels of lipid peroxides (looh), thiobarbituric acid reactive substances (tbars), carbonylproteins, total glutathione levels, and the activities of six antioxidant enzymes were measured in brain, liver, kidney and skeletal muscle of the common carp cyprinus carpio. hypoxia exposure (25% of normal oxygen level) for 5h generally decreased the levels of oxidative damage products, but in liver tbars content were elevated. hypox ... | 2005 | 15778094 |
| the complete genomic sequence of the carp fast skeletal myosin heavy chain gene. | we have determined the complete dna nucleotide sequence of the carp cyprinus carpio fast skeletal myosin heavy chain (myh) gene. introns and exons were predicted by comparison with the corresponding carp myh cdnas previously reported. the gene encoded the entire mrna transcript and contained 5958 nucleotides (nt) including 77 nt 5'-untranslated region, 5796 nt coding region for 1931 amino acids, and 85 nt 3'-untranslated region. the coding region was split by 38 introns and the complete gene con ... | 2005 | 15777658 |
| [anatomoradiological correlation of the hypothenar hammer syndrome]. | hypothenar hammer syndrome is all the clinical ischemic secondary manifestations in a stenose even one occlusion of traumatic origin of the distal portion of the ulnar artery. our objective was to underline anatomical substratum of this lesion and to emphasize the interest of the angio m.r.i. in the diagnosis of this syndrome. the radioanatomical study of the distal portion of the ulnar artery were on 10 fresh corpses of both sexes free of traumatic hurt of upperlimb. on 6 anatomical details inj ... | 2003 | 15776638 |
| recently published papers: from superbugs to superbowl. | several papers are discussed including the carp (coronary artery revascularisation prophylaxis) trial, examining the role, if any, of preoperative coronary revascularisation and a study examining the heart rate in the intensive care unit (icu) population. we also take a look at infections in the icu with particular regard to a recent study which examined isolating patients with methicillin-resistant staphylococcus aureus (mrsa). | 2005 | 15774060 |
| cloning of the koi herpesvirus (khv) gene encoding thymidine kinase and its use for a highly sensitive pcr based diagnosis. | outbreaks with mass mortality among common carp cyprinus carpio carpio and koi cyprinus carpio koi have occurred worldwide since 1998. the herpes-like virus isolated from diseased fish is different from herpesvirus cyprini and channel catfish virus and was accordingly designated koi herpesvirus (khv). diagnosis of khv infection based on viral isolation and current pcr assays has a limited sensitivity and therefore new tools for the diagnosis of khv infections are necessary. | 2005 | 15774009 |
| [genetic structure of crucian carp (cypriniformes, cyprinidae, carassius l. 1758) populations of middle-dnieper basin]. | comparative analysis of middle dnieper crucian carp's population structure was made by means of biochemical gene marking, cytometry and biological analysis. as a result the structure was found, which may by expounded like association of the genetically related species and forms. this association consists of diploid c. auratus (which predominates in population), c. carassius (which was found in the some of north-eastern reservoirs only) and triploid c. gibelio. in ukraine the homogeneous populati ... | 2008 | 15771088 |
| uniquely stable 40 kda subfragment-2 in carp myosin. | digestion of carp myofibrils at 30 degrees c in 0.5 m kcl medium with calcium ion generated unique 135 kda heavy meromyosin (hmm). the hmm was not produced when digested at 10 degrees c. a further digestion of the 135 kd hmm isolated in the absence of calcium ion generated uniquely short subfragment-2 (s-2) with a size of 40 kda (40 kda s-2) together with subfragment-1 (s-1). the 40 kda s-2 was identified by n- and c-end sequencing, and demonstrated to locate the amino end of the rod portion. th ... | 2005 | 15769163 |
| [effect of furosemide on gabaa-induced 36cl transport on atpase activity in synaptic membranes of carp (cyprinus carpio l.)]. | we studied the effect of furosemide on gabaa-induced 36cl transport and gabaa-induced cl- -atpase activity in synaptic membranes of fish brain. at physiological ph of 7.4, gaba (0.1-100 microm) stimulated 36cl transport in synaptoneurosomes and cl- -atpase activity in synaptic membranes. furosemide (0.1-0.5 mm) removed the activating effect of the mediator on chlorine transport and enzyme activity (i50 equaled 0.16 and 0.12 mm, respectively). in the absence of the mediator, picrotoxin (50 microm ... | 2016 | 15768629 |
| temperature alters the respiratory surface area of crucian carp carassius carassius and goldfish carassius auratus. | we have previously found that the gills of crucian carp carassius carassius living in normoxic (aerated) water lack protruding lamellae, the primary site of o(2) uptake in fish, and that exposing them to hypoxia increases the respiratory surface area of the gills approximately 7.5-fold. we here examine whether this morphological change is triggered by temperature. we acclimated crucian carp to 10, 15, 20 and 25 degrees c for 1 month, and investigated gill morphology, oxygen consumption and the c ... | 2005 | 15767311 |
| [phylogeny of chinese catfishes inferred from mitochondrial cytochrome b sequences]. | the mitochondrial dna cytochrome b gene was sequenced from 27 catfish species representing 11 families and 24 genera catfishes in china. aligning with cytochrome b sequences of eight catfish species from north america and africa retrieved from genbank, and selecting astyanax mexicanu, cyprinus carpio, and sardinops melanostictus as outgroups, we constructed a matrix of 38 dna sequences. the phylogenetic trees were constructed by using bayesian method and maximum parsimony (mp) method. the result ... | 2005 | 15759861 |
| [evidence of the unreduced diploid eggs generated from the diploid gynogenetic progeny of allotetraploid hybrids]. | following activation by uv-irradiated sperms from scatter scale common carp and without the treatment for doubling the chromosome number, the eggs generated by the diploid gynogenetic progeny (g1) with 100 chromosomes,developed into diploid gynogenetic progeny (g2) with 100 chromosomes. both the males and females of the tetraploids with 200 chromosomes were obtained from the offsprings (g1 x at) produced by mating the eggs of g1 with the diploid sperms from the allotetraploid hybrids. the result ... | 2005 | 15759860 |
| sandwich immunoassay for determination of vitellogenin in golden grey mullet (liza aurata) serum as a field exposure biomarker. | vitellogenin (vtg) is a protein produced in the liver of oviparous animals in response to oestrogens. abnormal production of vtg by males, therefore, is used as a biological indicator of exposure to xeno-oestrogens. in this study, a sandwich-elisa for measuring vtg in liza aurata (golden grey mullet) was developed and validated. plasma vtg was purified from 17beta-oestradiol-injected immature individuals of mullet, by size-exclusion and ion-exchange chromatography. polyclonal antibodies against ... | 2005 | 15756602 |
| differential spontaneous killing of human and murine tumour cell lines by leucocyte subpopulations from carp peripheral blood leucocytes. | cell populations from carp (cyprinus carpio l.) peripheral blood leucocytes (pbls) were examined for nonspecific cytotoxicities. by using monoclonal antibodies (mabs) against carp thrombocytes (tcl-hb8) and both neutrophils and monocytes (tcl-be8), pbls with a density of 1.08 g ml-1 were separated into three fractions: thrombocytes, a mixture of neutrophils and monocytes, and other cells (mainly lymphocytes), and the separated cells were tested for cytotoxic activities against mammalian tumour c ... | 2005 | 15752650 |
| long-term potentiation and olfactory memory formation in the carp (cyprinus carpio l.) olfactory bulb. | long-term potentiation of synaptic transmission is considered to be an elementary process underlying the cellular mechanism of memory formation. in the present study we aimed to examine whether or not the dendrodendritic mitral-to-granule cell synapses in the carp olfactory bulb show plastic changes after their repeated activation. it was found that: (1) the dendrodendritic mitral-to-granule cell synapses showed three types of plasticity after tetanic electrical stimulation applied to the olfact ... | 2005 | 15750817 |
| isolation of cdna of novel cytochrome p450 1b gene, cyp1b2, from carp (cyprinus carpio) and its induced expression in gills. | cytochrome p450 (cyp) enzymes constitute a multigene family of many endogenous and xenobiotic substances. the cyp1 family is of particular interest in environmental toxicology because its members are dominant in the metabolism of polycyclic aromatic hydrocarbons (pahs), polychlorinated biphenyls (pcbs), and aryl amines. a new complementary dna of the cyp1b subfamily encoding cyp1b2 was isolated from carp liver after intraperitoneal injection with 3-methylcholanthrene (3-mc). the obtained full-le ... | 2004 | 15750580 |
| temperature sensitivity of calcium binding for parvalbumins from antarctic and temperate zone teleost fishes. | parvalbumin (pv) is a soluble calcium-binding protein that is especially abundant in fast-twitch muscles of fish and other lower vertebrates. despite its prevalence in ectothermic taxa, few data address the effects of temperature on pv binding function. in this study, calcium dissociation constants (kd) were measured as a function of temperature (0-25 degrees c) for pv from two antarctic (gobionotothen gibberifrons and chaenocephalus aceratus) and two temperate zone fish species (cyprinus carpio ... | 2005 | 15748857 |
| ontogeny of gnrh-like immunoreactive neuronal systems in the forebrain of the indian major carp, cirrhinus mrigala. | gnrh immunoreactivity appeared in the medial olfactory placode very early in the development of cirrhinus mrigala. the immunoreactive elements were divisible into distinct migratory and non-migratory components. the migratory component appeared as a patch of intensely immunoreactive cells located close to the olfactory epithelium in day 6 post-fertilization larvae. subsequently, these neurons migrate caudally along the ventromedial aspect of the developing forebrain and enroute give rise to gnrh ... | 2005 | 15748717 |
| cdna cloning, sequence analysis and expression of 3-methylcholanthrene-inducible cytochrome p450 1b1 in carp (cyprinus carpio). | cytochrome p450 (cyp) genes, which make up a large gene superfamily, are known to play an important role in drug metabolism. the levels of expression of cyp genes in the tissue of fish inhabiting polluted areas have been used extensively in biomonitoring studies as indicators of dioxin pollution. complementary dna of cytochrome cyp1b1 was isolated from carp (cyprinus carpio) liver 24 h after the injection of 3-methylcholanthrene (3-mc). the full length cdna obtained contained a 5' noncoding regi ... | 2004 | 15746899 |
| effects of short- and long-term ultraviolet b irradiation on the immune system of the common carp (cyprinus carpio). | carp (cyprinus carpio) were repeatedly exposed to 0, 60, 120 and 240 mj/cm2 ultraviolet b (uvb) radiation three times in 1 week (short-term exposure) or 12 times in 4 weeks (long-term exposure). the effect of uvb on the functioning of the carp immune system was studied on day 2 after the final irradiation. after short-term uvb exposure, the whole-blood respiratory burst and cytotoxic activity were markedly enhanced, with parallel responses in both the number of circulating granulocytes and in th ... | 2013 | 15745420 |
| ultrasonographic findings of facial dysmorphism in wolf-hirschhorn syndrome. | wolf-hirschhorn syndrome has characteristic craniofacial dysmorphism with severe fetal growth restriction. we present a case that revealed facial dysmorphism characteristic of wolf-hirschhorn syndrome with two- and three-dimensional ultrasonography. the facial dysmorphic features in the present case were dolichocephaly; high forehead; large rectangular nose continuing to the eyebrows; hypertelorism; short philtrum; carp-shaped mouth; microretrognathia; and large, simply modeled, low-set ears. ch ... | 2005 | 15731989 |
| seasonal modulation of growth hormone mrna and protein levels in carp pituitary: evidence for two expressed genes. | adaptation of eurythermal fish to naturally varying environmental conditions involves modulation of expressions of various factors in the hypothalamo-hypophyseal axis. here we used three complementary approaches to assess the seasonal variation of growth hormone (gh) protein and mrna levels in pituitary glands of acclimatized carp fish. first, a polyclonal antibody raised against an oligopeptide derived from the carp gh sequence was used for immunohistochemistry; second, oligonucleotides specifi ... | 2005 | 15731922 |
| systemic priming of alloreactive cytotoxic cells in carp, following anal administration of allogeneic cell antigens. | in the present study, we confirmed that cellular immune responses, especially specific cell-mediated cytotoxicity, could be induced in systemic carp leucocytes, following anal administration of antigens. effector cells isolated from systemic lymphoid tissues (head kidney, spleen and peripheral blood) of carp that were immunised anally with allogeneic cells (epc or kg cell line) efficiently lysed immunogenic target cells. the lytic activity was increased as a result of secondary sensitisation and ... | 2005 | 15722230 |
| determination of select antidepressants in fish from an effluent-dominated stream. | increasing evidence indicates widespread occurrence of pharmaceuticals and personal care products (ppcps) in municipal effluent discharges and surface waters. studies that characterize the fate and effects of ppcps in aquatic systems are limited, and to our knowledge, data regarding pharmaceutical accumulation in fish of effluent-dominated ecosystems have not been previously reported. in the present study, fish populations were sampled from a reference stream and an effluent-dominated stream in ... | 2005 | 15720009 |
| immunoanalysis of antiviral mx protein expression in japanese flounder (paralichthys olivaceus) cells. | mx proteins are interferon-inducible gtpases that possess antiviral properties in vertebrates. japanese flounder (paralichthys olivaceus) mx protein has previously been shown to possess some antiviral activity against rhabdoviruses. a polyclonal antibody was generated against a purified peptide fragment of japanese flounder mx protein that had been produced in an escherichia coli expression system. the pab detected the approximately 71 kda mx protein from japanese flounder (hirame) natural embry ... | 2004 | 15707665 |
| molecular cloning of prepro-thyrotropin-releasing hormone cdnas from the common carp cyprinus carpio and goldfish carassius auratus. | to expand our knowledge on the evolution of prepro-thyrotropin-releasing hormone (pptrh) from fish to tetrapods, sequences of pptrh cdnas from two cyprinid teleosts, the common carp cyprinus carpio and goldfish carassius auratus, were determined. degenerate primers were designed based on the conserved regions between the zebrafish pptrh sequence identified from the zebrafish est database and the sockeye salmon pptrh sequence, and pcr amplification was performed. full-length pptrhs were confirmed ... | 2005 | 15707606 |
| ca2+-permeable and ca2+-impermeable ampa receptors coexist on horizontal cells. | fura-2 fluorescent calcium imaging was used for analyzing the subtype of ampa receptors in freshly dissociated horizontal cells of carp retina. exogenous application of ampa induced an increase of intracellular concentration of free ca2+ ([ca2+]i) in horizontal cells, while the [ca2+]i increase was partly inhibited by nifedipine. the residual [ca2+]i increase was completely eliminated by joro spider toxin-3, a blocker of ca2+-permeable ampa receptors. on the other hand, the application of pentob ... | 2005 | 15706232 |
| genetic relationships among members of the ichthyobodo necator complex: implications for the management of aquaculture stocks. | abstract ichthyobodo necator (costia) is a common and important flagellate parasite that infests the skin and gills of many freshwater and marine fish. costia infestations are often fatal and cause significant aquaculture losses worldwide. recently it has been demonstrated that ichthyobodo is a multispecies complex with differing host preferences. knowing if those species have broad or narrow host specificity has important implications for the management of costia. to address the question of hos ... | 2005 | 15705156 |
| the life cycle of henneguya nuesslini schuberg & schroder, 1905 (myxozoa) involves a triactinomyxon-type actinospore. | the life cycle of the histozoic myxozoan parasite henneguya nuesslini was investigated in two salmonid host species. naive brown trout, salmo trutta, and brook trout, salvelinus fontinalis, were experimentally infected in two trials by triactinomyxon type actinospores from naturally infected tubifex tubifex. in exposed common carp, cyprinus carpio, no myxospore production was detected. the parasite formed cysts with mature myxospores in the connective tissue of the fish 102 days post-exposure. t ... | 2005 | 15705152 |
| novel immunoglobulin-like transcripts in teleost fish encode polymorphic receptors with cytoplasmic itam or itim and a new structural ig domain similar to the natural cytotoxicity receptor nkp44. | members of the immunoglobulin superfamily (igsf) include a group of innate immune receptors located in the leukocyte receptor complex (lrc) and other small clusters such as the trem/nkp44 cluster. these receptors are characterised by the presence of immunoglobulin domains, a stalk, a transmembrane domain, and a cytoplasmic region containing either an immunoreceptor tyrosine-based inhibitory motif (itim) or are linked to an adapter molecule with an activation motif (itam) for downstream signallin ... | 2005 | 15702329 |
| epitheliocystis in carp (cyprinus carpio) in south korea. | epitheliocystis in the carp of a pet fish market were investigated by our diagnostic work and collecting information from department of laboratory animal medicine and fish & shellfish laboratory. the epitheliocystis was identified by using histopathological examination. epitheliocystis was confirmed as inflammation, epithelial hyperplasia, and lamellar fusion of the gill tissue. electron microscopic observation showed that the inclusions were filled with chlamydia-like organism. | 2005 | 15699608 |
| ankrd1 specifically binds casq2 in heart extracts and both proteins are co-enriched in piglet cardiac purkinje cells. | it has been suggested that the cardiac ankyrin repeat domain 1 protein (ankrd1), also known as carp, can play a pathophysiological role in the contractile responsiveness of myocardium. here, we study the potential functional roles of ankrd1 by searching for endogenous cardiac proteins that interact preferentially with ankrd1 in the heart-tissue extract from neonatal piglets, using non-biased pull-down approaches. these approaches identified, for the first time, a selective interaction between an ... | 2005 | 15698842 |
| effects of endocrine disrupters on sex steroid synthesis and metabolism pathways in fish. | the interactions of estrogenic (nonylphenol, dicofol, atrazine), androgenic (organotins, phthalates, fenarimol) and anti-androgenic compounds (vinclozolin, diuron, p,p'-dde) with key enzymatic activities involved in both synthesis and metabolism of sex hormones was investigated. carp testicular microsomes incubated in the presence of androstenedione and different xenobiotics evidenced higher sensitivity of 5alpha-reductase activity than 17beta-hydroxysteroid dehydrogenase activity towards those ... | 2004 | 15698553 |
| catalytic and structural characteristics of carp hepatopancreas d-amino acid oxidase expressed in escherichia coli. | d-amino acid oxidase of carp (cyprinus carpio) hepatopancreas was overexpressed in escherichia coli cells and purified to homogeneity for the first time in animal tissues other than pig kidney. the purified preparation had a specific activity of 293 units mg(-1) protein toward d-alanine as a substrate. it showed the highest activity toward d-alanine with a low km of 0.23 mm and a high kcat of 190 s(-1) compared to 10 s(-1) of the pig kidney enzyme. nonpolar and polar uncharged d-amino acids were ... | 2005 | 15694590 |
| whole-blood and plasma cholinesterase levels in normal koi (cyprinus carpio). | organophosphate and carbamate insecticides produce their toxic effects by binding with cholinesterase enzymes, resulting in the inhibition of acetylcholine catabolism. excessive acetylcholine levels cause excessive synaptic neurotransmitter activity. in most animal species, whole blood is the preferred sample for evaluation of cholinesterase activity. previous reports of cholinesterase activity in carp have involved sampling of serum, plasma, brain, liver, muscle, and heart. whole-blood samples ... | 2005 | 15690957 |
| development and evaluation of an experimental model of cutaneous columnaris disease in koi cyprinus carpio. | a reproducible, experimental model of columnaris disease was developed to study the pathogenesis of cutaneous disease associated with flavobacterium columnare infection in koi (cyprinus carpio). in experimental infections, lesions were usually restricted to skin and fins; gill necrosis was not a consistent finding. cytologic and histopathologic examinations provided a presumptive diagnosis of columnaris disease. specific detection of f. columnare was done using the polymerase chain reaction and ... | 2005 | 15690950 |
| comparative immunohistochemical study of carassius rfamide localization in teleost guts in different salinity habitats. | carassius rfamide (c-rfa) is a peptide, isolated originally from the brain of japanese crucian carp and sharing homologies with mammalian prolactin-releasing peptides. from the physiological aspect, it is known that c-rfa has contraction-promoting action on fish intestines, but its localization in peripheral tissues is unknown. we observed the localization of c-rfa in teleost guts using an immunohistochemical technique. c-rfa-like immunoreactive (irc-rfa) sites were observed in not only the smoo ... | 2005 | 15684584 |
| molecular cloning of leukocyte cell-derived chemotaxin 2 in rainbow trout. | in humans, leukocyte cell-derived chemotaxin 2 (lect2) is a 16kda chemotactic protein that consists of 133 amino acids and three intramolecular disulphide bonds. although it was originally demonstrated to have a chemotactic function in vitro, recent data sustain a further multifunctional role of lect2 that extends from cell growth, differentiation, damage/repair process and carcinogenesis to autoimmune diseases. the in vivo function of lect2 protein still remains obscure. in order to study the p ... | 2005 | 15683915 |
| partial oxidative-stress perturbs membrane permeability and fluidity of fish nucleated red blood cells. | we investigated the influence of partial oxidative stress on permeability and fluidity of nucleated fish red blood cells for simulating nucleated somatic cells. peroxide value indicating lipid hydroperoxide level in nucleated red blood cells of common carp (cyprinus carpio) increased with increasing body size. we detected that oxidation of nucleated red blood cells led to the degraded pufa compositions and accelerated the permeability of calcein and atp in the nucleated red blood cells restricte ... | 2004 | 15683836 |
| responses of molecular indicators of exposure in mesocosms: common carp (cyprinus carpio) exposed to the herbicides alachlor and atrazine. | common carp (cyprinus carpio) were treated in aquatic mesocosms with a single pulse of the herbicides atrazine or alachlor to study the bioavailability and biological activity of these herbicides using molecular indicators: liver vitellogenin gene expression in male fish for estrogenic activity, liver cytochrome p4501a1 gene expression, and dna damage in blood cells using the single-cell gel electrophoresis method. both alachlor and atrazine showed dose-related increases in dna strand breaks at ... | 2005 | 15683183 |
| description of an as yet unclassified dna virus from diseased cyprinus carpio species. | numerous deaths of koi and common carp (cyprinus carpio) were observed on many farms throughout israel, resulting in severe financial losses. the lethal viral disease observed is highly contagious and extremely virulent, but morbidity and mortality are restricted to koi and common carp populations. diseased fish exhibit fatigue and gasping movements in shallow water. infected fish had interstitial nephritis and gill necrosis as well as petechial hemorrhages in the liver and other symptoms that w ... | 2005 | 15681400 |
| a separation-free amperometric immunosensor for vitellogenin based on screen-printed carbon arrays modified with a conductive polymer. | a disposable amperometric immunosensor was studied for the rapid detection of carp (carassius auratus) vitellogenin (vtg). the sensor was fabricated based on screen-printed carbon arrays (spcas) containing eight carbon working and an integrated carbon counter electrodes. to construct the sensor, a conducting polymer (poly-terthiophene carboxylic acid) was electropolymerized on the surface of working electrodes and the polymer-coated spcas was characterized by sem. horseradish peroxidase (hrp) an ... | 2005 | 15681194 |
| avoidance behavior and brain monoamines in fish. | the crucian carp performs a typical avoidance behavior when exposed to olfactory cues from injured skin of conspecifics. they swim rapidly to the bottom and hide in available material. this work examines the effects of skin extract exposure and availability of hiding material on this behavior, and concomitant changes in brain monoaminergic activity in crucian carp. individual fish were exposed to skin extract in aquaria with or without hiding material. exposure to skin extract resulted in the ex ... | 2005 | 15680947 |
| inhibition of whole body ca2+ uptake in fresh water teleosts, channa punctatus and cyprinus carpio in response to salmon calcitonin. | in many fish, ultimobranchial-derived calcitonin (ct) has been shown to be a potent hypocalcemic regulator. but an equal number of studies failed to show any correlation between ct and plasma calcium levels. again, in fish, where ct has been shown to function as a hypocalcemic hormone, the way this is brought about is not well known. while the regulatory role of many hormones e.g., stanniocalcin, pituitary-derived prolactin and cortisol on gill calcium (ca2+) transport (gcat) has been well estab ... | 2004 | 15673109 |
| using mitochondrial nucleotide sequences to investigate diversity and genealogical relationships within common carp (cyprinus carpio l.). | direct sequencing of mitochondrial dna (mtdna) d-loop (745 bp) and mtatpase6/mtatpase8 (857 bp) regions was used to investigate genetic variation within common carp and develop a global genealogy of common carp strains. the d-loop region was more variable than the mtatpase6/mtatpase8 region, but given the wide distribution of carp the overall levels of sequence divergence were low. levels of haplotype diversity varied widely among countries with chinese, indonesian and vietnamese carp showing th ... | 2005 | 15670127 |
| toxicity and bioaccumulation of chlorpyrifos in indian carp catla catla (hamilton), labeo rohita (hamilton), and cirrhinus mrigala (hamilton). | 2004 | 15669740 | |
| elevated dna-protein cross-links in red blood cells of german carp, cyprinus carpio, from the lower passaic river, new jersey, usa. | 2004 | 15669735 | |
| opisthorchiasis from imported raw fish. | liver fluke infection caused by opisthorchiidae is a major public health problem in many parts of the far east, southeast asia, and eastern europe. however, with the growing volume of international travel and population migration, the infection is increasingly diagnosed in countries where the disease is not endemic, particularly in north america. we report an outbreak of acute opisthorchiasis in a family that was infected in a non-disease-endemic area after eating raw carp illegally imported fro ... | 2004 | 15663848 |
| noise emission during the first powerboat race in an alpine lake and potential impact on fish communities. | in order to assess the effects of high-speed boating on fish communities, noise levels were measured during the first class 1 powerboat race on the austrian lake traunsee. the noise spectra were compared to natural ambient noise and hearing abilities of four native fish species. sound pressure levels (spls) were significantly elevated during the training heats and the race compared with natural levels, reaching up to 128 db re 1 micropa (instantaneous spl) at a distance of 300 m to the powerboat ... | 2004 | 15658729 |
| effect of ultrasound on nucleated erythrocytes. | in this experimental study, carp erythrocytes were used as nucleated cell models to test the hypothesis that ultrasound (us) exposure can cause the change in plasma membranes and hemoglobin. to identify target cell damage, we studied hemolysis, osmotic fragility, lipid peroxidation of red blood cells and oxidation of hemoglobin in the erythrocytes. the erythrocytes were exposed to 1 mhz continuous-wave us at the intensities of 0.61 to 2.44 w/cm2 for 5 min. these results showed that us sonication ... | 2005 | 15653240 |
| genetic variability and relationships in mitochondrial dna co ii gene sequence of red common carps in china. | nucleotide sequence of cytochrome coxidase subunit ii (co ii) of mitochondrial dna ( mtdna) was analyzed from four populations of the red common carp (c. c. var. singuonensis, c. c. var. wananensis, c. c. var. wuyuanensis and c. c. var. color) as well as one population of wild common carp in china to detect their genetic variability and phylogenetic relationship. nineteen nucleotide variable positions were detected in analyzed 607 bp length sequence and 15 haplotypes were defined among five popu ... | 2004 | 15651674 |
| effect of hexavalent carcinogenic chromium on carp cyprinus carpio immune cells. | chromium is widely used in industrial processes, and is released into aquatic environments by electroplating, tannery and textile industries. fishes in natural waters or in aquaculture facilities supplied with these waters are exposed to chromium waste and are presumed to be affected by deposits. herein, we examine the effect of hexavalent chromium on carp cyprinus carpio derived immune cells. in vitro exposure of carp leukocytes to hexavalent chromium induced cytotoxicity, decreased mitogen-ind ... | 2004 | 15648842 |
| the cestode atractolytocestus huronensis (caryophyllidea) continues to spread in europe: new data on the helminth parasite of the common carp. | the caryophyllidean tapeworm atractolytocestus huronensis anthony, 1958, originally described from the common carp cyprinus carpio carpio l. from north america, has recently been introduced into europe (uk, hungary). in the present study, the dispersion of the tapeworm in other countries of central europe (slovakia and the czech republic) is described, including its first observation in the common carp of the tisa river, southeastern slovakia. because of the transboundary location of the river, ... | 2004 | 15648838 |
| identification of estrogenic compounds in wastewater effluent. | in order to identify the dominant contributors to estrogenic activity in environmental waters, a comprehensive fractionation method using silica gel column chromatography, combined with recombinant yeast assay for detecting estrogenic activity and with gas chromatography-mass spectrometry for quantifying endocrine disruptors and natural estrogens, was developed. the method was applied to the municipal sewage treatment plant (stp) secondary effluent discharged to the tamagawa river in tokyo, japa ... | 2004 | 15648753 |
| [experimental studies on the central analgesic effect of the non-steroidal anti-inflammatory drug carprofen in a sheep model -- preliminary results]. | to evaluate the effect of the non-steroidal anti-inflammtory drug carprofen on the lumbar cerebrospinal fluid (csf) concentration of noradrenaline (na) and serotonin (5-ht) in non-stimulated (part i) and surgically stimulated (part ii) sheep. | 2005 | 15645384 |
| effects of growth hormone on leucine absorption, intestinal morphology, and ultrastructure of the goldish intestine. | the mechanisms whereby exogenous growth hormone modulates intestinal structure and function in fish were investigated. goldfish (carassius auratus) were fed commercial flake diet sprayed with recombinant carp growth hormone (cgh) daily for 1 month. control animals received food sprayed with the vehicle. after 1 month of daily feedings, body mass and length were determined, and animals were sacrificed to study intestinal characteristics. sections of foregut were removed after determination of tot ... | 2004 | 15644934 |
| impact of pcb mixture (aroclor 1254) and tbt and a mixture of both on swimming behavior, body growth and enzymatic biotransformation activities (gst) of young carp (cyprinus carpio). | the sensitivity of carp to chronical stress due to single and combined mixtures (each 50%) of tbt and pcb was evaluated under laboratory conditions. and concluded, mixed exposures were partially more or less toxic than single ones. xenobiotic stress led to both decreased mean daily swimming speed and an increased mean swimming speed during darkness. also a decreased preferred swimming depth (nearer to the surface) during nighttime was observed. we found approximately synergistic effects of tbt a ... | 2005 | 15642631 |
| clinical trials update from the american heart association meeting: acorn-csd, primary care trial of chronic disease management, peace, create, shield, a-heft, gemini, vitamin e meta-analysis, escape, carp, and scd-heft cost-effectiveness study. | this article provides information and a commentary on landmark trials presented at the american heart association meeting held in november 2004, relevant to the pathophysiology, prevention, and treatment of heart failure. an open trial of the acorn cardiac support device (csd) showed encouraging preliminary results in patients with severe heart failure. the peace (prevention of events with angiotensin-converting enzyme inhibition) study supports data from previous studies showing that ace inhibi ... | 2005 | 15642544 |
| role of ca2+ store in ampa-triggered ca2+ dynamics in retinal horizontal cells. | fura-2 fluorescent calcium imaging was applied to measure [ca(2+)](i) in freshly dissociated horizontal cells of carp retina, and a model containing endoplasmic reticulum (er) membrane processes and plasma membrane processes was constructed for quantitative analyses of the ampa-triggered calcium dynamics. a transient increase followed by a sustained steady level of [ca(2+)](i) was observed when 100 microm ampa was applied, while the initial transient increase of [ca(2+)](i) was suppressed by exo ... | 2004 | 15640746 |
| [the analysis of genetic variability of mtdna 12s rrna genes in the allotetraploid, triploid crucian carp and their parents]. | the complete 12s rrna genes from the five f11 allotetraploids, two diploid gynogens, two triploid crucian carps, one red crucian carp, one xiangjiang common carp, and one japanese crucian carp were successfully cloned and sequenced. sequence compositions and variations were analyzed with mega 1.0 software. five f11 allotetraploids shared two haplotypes, and two diploid gynogens, two triploid crucian carps, one red crucian carp, one xiangjiang common carp, one japanese crucian carp shared one hap ... | 2004 | 15640120 |
| plasma alpha-msh and acetylated beta-endorphin levels following stress vary according to crh sensitivity of the pituitary melanotropes in common carp, cyprinus carpio. | pituitary melanotropes release alpha-melanocyte-stimulating hormone (alpha-msh) and acetylated beta-endorphin (nac beta-end) during stress responses. however, effects of stressors on plasma concentrations of these hormones are highly inconsistent among fish species. here, we show that also within a species, the common carp (cyprinus carpio), fish sometimes respond with elevated alpha-msh and nac beta-end plasma levels, and at other times not. the origin of this variable response was investigated ... | 2005 | 15639149 |
| melatonin accelerates maturation inducing hormone (mih): induced oocyte maturation in carps. | the present communication is an attempt to demonstrate the influence of melatonin on the action of maturation inducing hormone (mih) on the maturation of oocytes in carps. the oocytes from gravid female major carp labeo rohita were isolated and incubated separately in medium 199 containing (a) only mih (1 microg/ml), (b) only melatonin (at concentrations of 50, 100 or 500 pg/ml), and (c) both melatonin and mih, but at different time intervals. in the latter group, melatonin was added to the incu ... | 2004 | 15639142 |
| dna aggregating protein from carp (cyprinus capio) eggs. | hemagglutinating activity for glutaraldehyde-fixed, trypsinized human and rabbit erythrocytes was obtained from carp (ciprinus capio) egg extract. this activity was inhibited by sulfated glycans such as heparin and fucoidan, and salmon testis dna (stdna), but not by mono- and oligosaccharides. the active fraction from hydroxyapatite column, ha400, caused aggregation of stdna at a concentration of 125 microg/ml. ha400-induced aggregation was inhibited by heparin and fucoidan at the concentrations ... | 2005 | 15635155 |
| carp, a cardiac ankyrin repeat protein, is up-regulated during wound healing and induces angiogenesis in experimental granulation tissue. | cardiac ankyrin repeat protein (carp) was identified by subtractive hybridization as one of a group of genes that are rapidly modulated by acute wounding of mouse skin. quantitative rt-pcr showed that carp was strongly induced during the first day after wounding (157.1-fold), and the high level persisted for up to 14 days. immunohistochemistry and in situ hybridization revealed that carp was expressed in skeletal muscle, vessel wall, hair follicle, inflammatory cells, and epidermis in the wound ... | 2005 | 15632022 |
| carp liver dnase--isolation, further characterization and interaction with endogenous actin. | deoxyribonuclease i (dnase i)-like enzyme from the liver of the carp (cyprinus carpio) was purified to homogeneity and further characterized. ion exchange chromatography on deae-cellulose, molecular filtration on sephacryl s-300 and con a-sepharose affinity chromatography were applied for enzyme isolation. carp liver dnase, similarly to dnase i from bovine pancreas, was found to be an endonuclease that hydrolyses linear dna from salmon sperm as well as circular dna forms--plasmid and cosmid. the ... | 2005 | 15621519 |
| pentachlorophenol reduces b lymphocyte function through proinflammatory cytokines in carassius auratus. | cytokines and igm levels are important parameters in the acquired immunity of fish. in the present study, the effect of pentachlorophenol (pcp) on the expression of proinflammatory cytokines, tnf-alpha (tumor necrosis factor-alpha) and il-1beta (interleukin 1) mrna levels of crucian carp, were determined by semi-quantitative reverse transcriptase-polymerase chain reaction (rt-pcr) method. to put the deduction of pcp's immunotoxicity on b cell function, b cells secretion of igm under exposure of ... | 2005 | 15621336 |
| brain responses to ambient temperature fluctuations in fish: reduction of blood volume and initiation of a whole-body stress response. | spatial and temporal ambient temperature variations directly influence cellular biochemistry and thus the physiology of ectotherms. however, many aquatic ectothermic species maintain coordinated sensorimotor function during large acute body-temperature changes, which points to a compensatory mechanism within the neural system. here we used high-resolution functional magnetic resonance imaging to study brain responses to a drop of 10 degrees c of ambient water temperature in common carp. we obser ... | 2005 | 15615828 |
| the strengths of in vivo magnetic resonance imaging (mri) to study environmental adaptational physiology in fish. | adaptational physiology studies how animals cope with their environment, even if this environment is subject to permanent fluctuations such as tidal or seasonal variations. aquatic organisms are generally more prone to be exposed to osmotic, hypoxic and temperature challenges than terrestrial animals. some of these challenges are more restraining in an aquatic environment. to date, very few studies have used in vivo magnetic resonance imaging (mri) to uncover the physiological mechanisms that re ... | 2004 | 15614515 |
| reproductive responses of common carp (cyprinus carpio) exposed in cages to influent of the las vegas wash in lake mead, nevada, from late winter to early spring. | the las vegas wash (lw) delivers tertiary-treated municipal wastewater effluent, nonpotable shallow groundwater seepage, and runoff from the urbanized las vegas valley to las vegas bay (lx) of lake mead. to investigate the potential for contaminants in lw influent to produce effects indicative of endocrine disruption in vivo, adult male and female common carp (cyprinus carpio) were exposed in cages for 42-48 d at four sites in lake mead: lw, lx, and two reference locations in the lake. end point ... | 2004 | 15597896 |
| aspartic acid scanning mutation analysis of a goldfish growth hormone-releasing hormone (ghrh) receptor specific to the ghrhsalmon-like peptide. | growth hormone-releasing hormone (ghrh) plays a pivotal role in the regulation of growth. the study of goldfish ghrh and its receptor is of particular interest as it is so far the only animal model in which two forms of ghrh-like (catfish-like and salmon-like) peptides coexist, and these peptides share only 30-40% of amino acid sequence identities with their mammalian counterparts. for these reasons, we have previously characterized a goldfish ghrh receptor, which is specific for a synthetic car ... | 2005 | 15596070 |
| molecular phylogeny of the kidney-parasitic sphaerospora renicola from common carp (cyprinus carpio) and sphaerospora sp. from goldfish (carassius auratus auratus). | the genetic relatedness of two kidney-parasitic sphaerospora species was studied. although s. renicola, the causative agent of swimbladder inflammation of common carp fingerlings (cyprinus carpio), and sphaerospora sp. originating from goldfish (carassius auratus auratus) were indistinguishable on the basis of spore morphology, they were found to be genetically different as their 18s rdna sequences shared only 71.9% identical nucleotides. in the phylogenetic trees, sphaerospora sp. from goldfish ... | 2004 | 15595280 |
| improvement of transfection efficiency of epithelioma papulosum cyprini carp cells by modification of cell cycle and use of an optimal promoter. | several methods to improve transfection of epithelioma papulosum cyprini (epc) carp cells have been tested and are reported here. by modifying the cell cycle state of epc cell monolayers and selecting the best promoter for the plasmid to be transfected, we increased transfection efficiency from 12.8% to 55.1% and decreased the coefficient of variation among different experiments from 54.1% to 11.8%. thus 2- to 3-fold higher transfection efficiencies were obtained when the epc monolayers were tre ... | 2004 | 15592944 |
| ultrastructural identification of a herpes-like virus infection in common carp cyprinus carpio in korea. | we report on a herpes-like virus, which was found to be associated with mass mortality of common carp cyprinus carpio for the first time in korea in 1998. the external signs of infection in moribund fish were darkened coloration and severe branchial necrosis in the gill. transmission electron microscopy revealed the presence of herpes-like viruses in spleen tissue. infected spleen cells showed hypertrophied nuclei and degeneration. numerous nucleocapsids of about 82 nm in diameter were found wit ... | 2004 | 15584424 |
| a novel serine protease complexed with alpha2-macroglobulin from skeletal muscle of lizard fish (saurida undosquamis). | a novel fish muscle serine protease named muscle soluble serine protease (mssp) was purified from the soluble fraction of lizard fish (saurida undosquamis: synodontidae) muscle by ammonium sulfate fractionation followed by four steps of column chromatographies. in native-page, the purified enzyme appeared as a single band with an estimated mol. mass of approximately 380 kda by gel filtration. in sds-page under reducing conditions, the purified enzyme migrated as two protein bands at 110 and 100 ... | 2004 | 15581796 |
| sequence and expression of a constitutive, facilitated glucose transporter (glut1) in atlantic cod gadus morhua. | a putative glucose transporter, glut1, is reported for atlantic cod gadus morhua. a combination of rt-pcr, rlm-race and genome walking were used to articulate a 4560 bp cdna (genbank accession number ay526497). it contains a 149 bp 5' utr, a 1470 bp open reading frame and a 2941 bp 3' utr. at the nucleotide level, the cod glut1 orf shares 78.2% sequence identity to human glut1 and the deduced amino acid sequence clusters with glut1s from rainbow trout and carp. glut1 transcript is highly express ... | 2004 | 15579563 |
| genetic variation analysis within and among six varieties of common carp (cyprinus carpio l.) in china using microsatellite markers. | five microsatellites were used to study the genetic diversity and genetic structure of one wild and five domestic varieties of common carp in china (the yangtze river wild common carp, xingguo red carp, purse red carp, qingtian carp, russian scattered scaled mirror carp and japanese decorative carp). all loci in this study showed marked polymorphism with the number of alleles from 4 to 13. domestic varieties (except xingguo red carp) showed less genetic diversity than the yangtze river wild comm ... | 2004 | 15575506 |
| complete nucleotide sequence of the hirame rhabdovirus, a pathogen of marine fish. | reverse transcription-polymerase chain reaction (rt-pcr) derived clones were constructed for the hirame rhabdovirus (hirrv) ca-9703 strain from korea, and the dna was sequenced. the 3'-end of genomic rna was cloned by poly(a)-tailing of the genomic rna before reverse transcription, and the 5'-end of the genome was cloned by poly(g)- or poly(c)-tailing of the first strand, followed by pcr. the remainder of the genomic dna was cloned by reverse transcription-polymerase chain reaction using primers ... | 2005 | 15567027 |
| the application of image cytometry to viability assessment in dual fluorescence-stained fish spermatozoa. | the viability of spermatozoa has been assessed using sybr 14 staining for dna of living cells and propidium iodide staining for dna of degenerate cells. this dual staining was performed on four fish species (siberian sturgeon, acipenser baerii; common carp, cyprinus carpio; tench, tinca tinca and wels, silurus glanis) and the proportions of live and dead spermatozoa were assessed by epifluorescence microscopy and image cytometry. ten phase contrast and epifluorescent images were recorded per sam ... | 2004 | 15566965 |
| annual evolution of fatty acid profile from muscle lipids of the common carp (cyprinus carpio) in madagascar inland waters. | annual evolution of muscle lipids fatty acid (fa) from common carp (cyprinus carpio) has been determined in 2001 through monthly samplings in the reserve pond of sisaony (sis series) and itasy lake (ita series) of the madagascar highlands. total lipids from muscle were extracted and quantified according to the bligh and dyer method. fa identification was performed by gc-ms of fa methyl esters and fa pyrrolidides and led to the identification of 41 fa; routine analyses of fa were made by capillar ... | 2004 | 15563217 |