Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| accuracy of fecal lactoferrin and other stool tests for diagnosis of invasive diarrhea at a colombian pediatric hospital. | estimate under "real life" conditions the operating characteristics of several stool tests for determining whether a diarrheal episode is invasive-inflammatory. | 1999 | 10223687 |
| the electrophoretic karyotype of entamoeba histolytica. | although knowledge about gene organization and transcription control in the protozoan parasite entamoeba histolytica has increased substantially during the last few years, questions remain open about ploidy, organization and number of chromosomes in this human pathogen. to get insight into these questions conditions were elaborated to consistently separate e. histolytica chromosomes using pulsed-field gel electrophoresis. southern blot analyses indicated variations in number and size of homologo ... | 1999 | 10215023 |
| different antiamebic antibody isotype patterns in the large and small intestine after local and systemic immunization of mice with glutaraldehyde fixed entamoeba histolytica trophozoites. | we have determined the major immunoglobulin isotypes (igg, iga, igm) of antiamebic antibodies induced in the serum and in the large and small intestine after local (oral and rectal) or systemic (intraperitoneal and intramuscular) immunization of mice with glutaraldehyde-fixed entamoeba histolytica trophozoites (gft). iga predominated in the small intestine after immunization through all routes, whereas in the large intestine similar antibody levels of the major isotypes were induced by rectal, i ... | 1999 | 10210270 |
| the tata-box binding protein of entamoeba histolytica: cloning of the gene and location of the protein by immunofluorescence and confocal microscopy. | a 309 bp dna fragment from entamoeba histolytica was amplified by pcr using primers derived from the acanthamoeba castellanii consensus tata-box binding protein amino acid sequence. the amplified fragment was used to isolate cdna and genomic dna clones containing an orf encoding the complete e. histolytica tata-box binding protein (ehtbp, 702 bp, 234 aa, molecular mass 26 kda). the ehtbp functional domain showed 55% sequence identity to that of homo sapiens, 54% to a. castellanii and 37% to plas ... | 1999 | 10206708 |
| [amoebic dysentery]. | 1999 | 10201245 | |
| antiprotozoal properties of helianthemum glomeratum. | structure characterization and biological evaluation of the compounds isolated from helianthemum glomeratum, particularly that of the polyphenols, has been the aim of a series of studies carried out to define the further potential use of this plant in the treatment of infectious diarrhoea in children. the flavan-3-ols, (-)-epigallocatechin and (-)-epigallocatechin gallate, isolated from helianthemum glomeratum roots were tested for their antiamoebic and antigiardial effects in vitro. compared wi ... | 1999 | 10190180 |
| characterization of ys-27, an axenic korean strain of entamoeba histolytica. | characterization of ys-27, an axenic entamoeba strain, was performed by three different laboratory methods. zymodeme analysis using starch gel electrophoresis and pcr with species-specific primers showed that ys-27 is a pathogenic entamoeba which belongs to the group ii zymodeme. pathogenicity of ys-27 was further confirmed by observing the formation of liver abscess in mongolian gerbils. these results showed that ys-27 is e. hisolytica. | 1999 | 10188386 |
| still around and still dangerous: giardia lamblia and entamoeba histolytica. | the discovery of new infectious agents often overshadows the continuing impact and importance of well-established organisms. in the case of diarrheal disease, entamoeba histolytica and its complications remain a major cause of morbidity and mortality in developing countries. although it is also present in developing countries, giardia lamblia is a primary cause of waterborne outbreaks of diarrhea in developed countries. persons at risk of developing giardiasis in these countries include backpack ... | 1997 | 10177206 |
| application of an elisa method for screening antibody levels to entamoeba histolytica. | 1990 | 10150142 | |
| [pleuropulmonary manifestations of amebiasis]. | amibiasis is the third leading cause of death due to parasitic infections in the world. amibiasis is endemic in the warm regions of the world with deficient hygiene and socio-economic situations. entamoeba histolytica is the causal agent of invasive amibiasis, unlike entamoeba dispar which is not a pathogen for humans. amibian colitis and amibian abscess of the liver are the most frequent intestinal and extra-intestinal manifestations. pleuropulmonary complications almost always occur in patient ... | 1998 | 10100347 |
| crystallization and preliminary x-ray studies of a recombinant calcium-binding protein from entamoeba histolytica. | a calcium-binding protein (cabp) of entamoeba histolytica was purified from an e. coli recombinant clone carrying the cabp gene in a pet-3c expression vector using anion-exchange and size-exclusion chromatography. examination of the amino-acid sequence of the recombinant protein suggested that it has four independent ef-hand motifs. the protein dissolved in cacodylate buffer was crystallized using the hanging-drop method with 2-methylpentane-2,4-diol (mpd) as the precipitant. x-ray diffraction d ... | 1998 | 10089533 |
| myosin ib from entamoeba histolytica is involved in phagocytosis of human erythrocytes. | entamoeba histolytica is a protozoan parasite that causes amoebic dysentery in humans. the disease is prevalent worldwide. infection with e. histolytica results in invasion of the intestine by the parasite, followed by tissue damage and inflammation. during this invasive process, parasites kill and phagocytose human epithelial cells, immune cells and erythrocytes. expression of amoebic pathogenicity requires a dynamic cytoskeleton that allows movement, tissue penetration and changes in parasite ... | 1999 | 10085254 |
| antiamoebic and antigiardial activity of plant flavonoids. | the in vitro antiprotozoal activity of 18 natural flavonoids has been determined against entamoeba histolytica and giardia lamblia. (-)-epicatechin, (-)-epigallocatechin and kaempferol were the most active flavonoids against both protozoa. | 1999 | 10083850 |
| prevalence of intestinal parasite infections with special reference to entamoeba histolytica on the island of bioko (equatorial guinea). | the prevalence of intestinal parasitic infections was assessed (1993 through 1995) among two different groups of persons on the island of bioko, equatorial guinea. in the first group, parasitologic examinations were performed on stool specimens from a household-based sample of 557 dwellers from the rural area of the island. in the second group, 1,633 inpatients and outpatients at the general hospital of malabo (the capital of the country) were studied. all age groups were represented in both gro ... | 1999 | 10072147 |
| isoenzyme and molecular characterization of entamoeba histolytica and entamoeba dispar isolates from symptomatic and asymptomatic subjects. | to correlate the clinical features of amebic infections with the characteristics of entamoeba culture isolates of stools. | 1999 | 10063741 |
| etiology of outpatient pediatric nondysenteric diarrhea: a multicenter study in the united states. | few data have been published recently on the etiology of outpatient pediatric diarrhea in the us. | 1999 | 10048678 |
| l-myo-inositol 1-phosphate synthase from entamoeba histolytica. | l-myo-inositol 1-phosphate synthase (i-1-p synthase) catalyses the primary reaction for the synthesis of inositol in a variety of prokaryotes, eukaryotes and in the chloroplasts of algae and higher plants. inositol is a precursor of essential macromolecules like membrane phospholipids, gpi anchor proteins and lipophosphoglycans, which play a determinant role in the pathogenesis of protozoan parasites such as leishmania and entamoeba. however, there is no report of i-1-p synthase or its gene from ... | 1999 | 10029310 |
| hsp60 is targeted to a cryptic mitochondrion-derived organelle ("crypton") in the microaerophilic protozoan parasite entamoeba histolytica. | entamoeba histolytica is a microaerophilic protozoan parasite in which neither mitochondria nor mitochondrion-derived organelles have been previously observed. recently, a segment of an e. histolytica gene was identified that encoded a protein similar to the mitochondrial 60-kda heat shock protein (hsp60 or chaperonin 60), which refolds nuclear-encoded proteins after passage through organellar membranes. the possible function and localization of the amebic hsp60 were explored here. like hsp60 of ... | 1999 | 10022906 |
| entamoeba histolytica: signaling through g proteins. | the intracellular signaling pathways of entamoeba histolytica are largely unknown. although the expression of guanine nucleotide binding proteins (g proteins) is expected from functional studies, their biochemical characterization remains elusive in this protozoan. using a combination of biochemical and immunological studies, we provide strong evidence for the presence of a gs protein in amoeba. our results strengthen our understanding of the signal transduction mechanisms in e. histolytica as p ... | 1999 | 9990345 |
| bacterial expression of a neutralizing mouse monoclonal antibody fab fragment to a 150-kilodalton surface antigen of entamoeba histolytica. | a mouse monoclonal antibody (mab) (eh3015, igg1 with a k light chain) prepared by hybridoma technology recognizes a 150-kd surface antigen of entamoeba histolytica and inhibits adherence and cytotoxicity of the ameba to mammalian cells. the genes encoding the light chain and the fd region of the heavy chain of the mab were cloned and expressed in escherichia coli. the plasmid used was designed for the expression of fab with a hexa-histidine tag in the periplasmic space. recombinant fab fragments ... | 1999 | 9988319 |
| anaemia and intestinal parasitic infections among school age children in behera governorate, egypt. behera survey team. | anaemia is considered a serious public health problem in egypt, although updated population-based data are lacking. similarly, data on prevalence and intensity of infection with intestinal parasites, which are considered one possible cause of anaemia, are available only from small, unrepresentative sample surveys. the present research was implemented on an entire governorate representative sample. the aim of the study was to assess the prevalence of anaemia and intestinal parasites in the area a ... | 1998 | 9972072 |
| protection of hamsters from amebic liver abscess formation by a monoclonal antibody to a 150-kda surface lectin of entamoeba histolytica. | we examined the effects of passive immunization with a monoclonal antibody (eh3015) that recognizes a 150-kda surface lectin of entamoeba histolytica on amebic liver-abscess formation in hamsters. the hamsters were inoculated i.p with 0.1, 1.0, or 10 mg of eh3015 at 24 h prior to an intrahepatic challenge with 10(5) trophozoites of e. histolytica. in hamsters treated with 1.0 and 10 mg of eh3015 the incidence of liver abscesses was significantly reduced. these results demonstrate that monoclonal ... | 1999 | 9950232 |
| interactions between epithelial cells and immune cells in the intestine. | not merely a passive barrier as previously assumed, epithelial cells act as sensitive indicators of infection that initiate defense responses. noninvasive as well as invasive organisms have been demonstrated to elicit production of chemoattractants. that noninvasive organisms have this capacity suggests that receptor-mediated signaling pathways may be involved. epithelial cells have been found to release both chemokines and cytokines in a precise mixture that varies according to the origin or ma ... | 1998 | 9928368 |
| overdiagnosis of intestinal amoebiasis associated to serial microscopical examination of faeces. some precisions on a problem. | 1998 | 9921305 | |
| [laboratory diagnosis of protozoan and parasitic infections. 1. protozoan infections. b. amebic dysentery]. | 1998 | 9921248 | |
| cloning and bacterial expression of adenosine-5'-triphosphate sulfurylase from the enteric protozoan parasite entamoeba histolytica. | a gene encoding adenosine-5'-triphosphate sulfurylase (as) was cloned from the enteric protozoan parasite entamoeba histolytica by polymerase chain reaction using degenerate oligonucleotide primers corresponding to conserved regions of the protein from a variety of organisms. the deduced amino acid sequence of e. histolytica as revealed a calculated molecular mass of 47925 da and an unusual basic pi of 9.38. the amebic protein sequence showed 23-48% identities with as from bacteria, yeasts, fung ... | 1998 | 9920405 |
| amoebapore homologs of caenorhabditis elegans. | it is shown that the caenorhabditis elegans genome contains several distantly related members of the gene family of saposin-like proteins. the putative products of genes t07c4.4, t08a9.7a, t08a9.7b, t08a9.8, t08a9.9, t08a9.10 are similar to the amoebapores of entamoeba histolytica, granulysin of cytotoxic t lymphocytes and a putative amoebapore-related protein of the liver fluke fasciola hepatica inasmuch as they consist of only a single saposin-like domain and a secretory signal peptide. the sa ... | 1998 | 9920402 |
| detection of circulating antigen in amoebic liver abscess by counter-current immunoelectrophoresis. | fifty serum samples from patients with amoebic liver abscess and 50 from subjects who had not suffered from the disease (25 from patients with other, chiefly parasitic, infections and 25 from healthy blood donors, staff or students) were tested for circulating amoebic antigen by counter-current immuno-electrophoresis (ciep). amoebic antigen was detected in 38 sera (76%) from cases of amoebic liver abscess, but in none of the other sera. although ciep is only moderately sensitive, the high specif ... | 1999 | 9920132 |
| evaluation of laboratory techniques for diagnosis of amoebiasis. | evaluation of 82 cases of amoebiasis including 4 of liver abscess was done. microscopy of 62 stool samples revealed trophozoites in 19 (30.6%) and cysts in 8 (12.9%). pva preserved samples were stained by iron-hamatoxylin and trichrome. twenty-nine samples (46.7%) were positive by culture on endamoeba, nih and charcoal agar. additional two culture positive cases were negative by direct microscopy. three (75%) samples of liver abscess pus were culture positive. of the sera screened for amoebic an ... | 1998 | 9914676 |
| giardia lamblia: in-vitro sensitivity to some chemotherapeutic agents. | the susceptibility of giardia lamblia to eight chemotherapeutic agents was studied in vitro. the criteria of viability of the parasite was the ability of the parasite to attach to the coverslip surface in situ, and to multiply in drug free media on subcultures. the giardicidal and 50% inhibitory concentrations of each drug were measured. the activity of metronidazole in vitro was found to be similar to that of mepacrine, whilst that of chloroquine was inferior. the sensitivity of g. lamblia to m ... | 1998 | 9914672 |
| inhibition of entamoeba histolytica proteolytic activity by human salivary iga antibodies. | entamoeba histolytica is a protozoan parasite that causes amoebiasis in humans; as the infection occurs mainly in the intestinal epithelium, the secretory immune response of the host could have an influence on the outcome. secretory iga antibodies against e. histolytica have been detected in asymptomatic and symptomatic patients, but little is known about their protective role. e. histolytica cysteine proteases seem to be involved in the pathogenesis of amoebiasis; therefore, it is important to ... | 1998 | 9890272 |
| etiologies of acute, persistent, and dysenteric diarrheas in adults in bangui, central african republic, in relation to human immunodeficiency virus serostatus. | a study of the etiologies of diarrhea in adults in relation to their human immunodeficiency virus (hiv) serostatus and number of cd4+ cells was carried out in the central african republic. in cases and controls, multi-parasitism was observed. salmonella spp. were identified mainly during acute diarrhea, with 50% of the s. enteritidis isolated during the study being responsible for septicemia and/or urinary tract infection in immunodeficient patients. enteroaggregative escherichia coli (eaggec) w ... | 1998 | 9886215 |
| field study on the distribution of entamoeba histolytica and entamoeba dispar in the northern philippines as detected by the polymerase chain reaction. | we used the polymerase chain reaction (pcr) to study the distribution of entamoeba histolytica and e. dispar in 1,872 individuals in 14 communities in the northern philippines. here we report a field study using a dna extraction protocol from formalin-fixed stool specimens as previously reported. this assay detected 137 stools (7.318%) containing e. dispar and 18 stools (0.961%) containing e. histolytica. the most affected age group for e. histolytica/e. dispar infections were those 5-14 years o ... | 1998 | 9886200 |
| molecular cloning and characterization of the genes encoding two isoforms of cysteine synthase in the enteric protozoan parasite entamoeba histolytica. | the enteric protozoan parasite entamoeba histolytica was shown to possess cysteine synthase (cs) activity. the cdna and genomic clones that encode two isoforms of the e. histolytica cs were isolated and characterized from a clonal strain of e. histolytica by genetic complementation of the cysteine-auxotrophic escherichia coli nk3 with an e. histolytica cdna library. the two types of the e. histolytica cs genes differed from each other by three nucleotides, two of which resulted in amino acid sub ... | 1998 | 9879885 |
| infection and immunity mediated by the carbohydrate recognition domain of the entamoeba histolytica gal/galnac lectin. | entamoeba histolytica causes invasive amebiasis, a major parasitic disease of the developing world, whose primary symptoms are liver abscess and colitis. all strains of e. histolytica express a 260-kda surface gal/galnac lectin that is antigenically conserved and immunogenic. the lectin is required for adherence to human intestinal epithelial cells and contact-dependent killing of immune effector cells. by expression cloning, the carbohydrate recognition domain (crd) was identified within the le ... | 1999 | 9878032 |
| induction of a spectroscopically defined transition by guanidinium hydrochloride on a recombinant calcium binding protein from entamoeba histolytica. | sequence analysis and metal ion binding studies reported earlier have established that the calcium binding protein (cabp) from the parasitic ameboid entamoeba histolytica protein has four canonical ef hand motifs which bind calcium. equilibrium denaturation studies on both the apo and the holo forms of this protein indicate the presence of stable transition intermediates at low denaturant concentrations as revealed by the binding of the non-specific hydrophobic dye ans. fast reaction kinetics sh ... | 1998 | 9877168 |
| efficacy of new 5-nitroimidazoles against metronidazole-susceptible and -resistant giardia, trichomonas, and entamoeba spp. | the efficacies of 12 5-nitroimidazole compounds and 1 previously described lactam-substituted nitroimidazole with antiparasitic activity, synthesized via srn1 and subsequent reactions, were assayed against the protozoan parasites giardia duodenalis, trichomonas vaginalis, and entamoeba histolytica. two metronidazole-sensitive lines and two metronidazole-resistant lines of giardia and one line each of metronidazole-sensitive and -resistant trichomonas were tested. all except one of the compounds ... | 1999 | 9869568 |
| 69-year-old man with fever, diarrhea, and abdominal pain. | 1998 | 9868422 | |
| ribosomal dna fragments enhance the stability of transfected dna in entamoeba histolytica. | genetic manipulation of entamoeba histolytica is limited by the inability to express foreign genes at high levels. we tested whether sequences from the e. histolytica rdna episome, present in 200 copies per cell, could act to stabilize the episomal transfection vector ptcv1. ligation of the rdna transcription unit, or sequences downstream of the rdna transcription unit, increased ptcv1 copy number and stability and conferred additional zones of dna replication. sequences upstream of the rdna tra ... | 1998 | 9864856 |
| antisense inhibition of expression of cysteine proteinases affects entamoeba histolytica-induced formation of liver abscess in hamsters. | trophozoites of virulent entamoeba histolytica transfected with the antisense gene encoding cysteine proteinase 5 (cp5) have only 10% of the cp activity but retain their cytopathic activity on mammalian monolayers. in the present study we found that the transfected trophozoites with low levels of cp activity were incapable of inducing the formation of liver lesions in hamsters. | 1999 | 9864246 |
| effect of bacterial association on virulence of entamoeba histolytica to baby hamster kidney cell monolayers. | axenic e. histolytica trophozoite strain nih:200 and hmi:imss when co-associated with aerobic bacteria escherichia coli strain k12 and serotype 056 showed marked increase in virulence as observed by destruction of baby hamster kidney (bhk) monolayers. however, when incubated with anaerobic bacterial strains clostridium perfringens and bacteroides fragilis virulence remained unaltered. further, adherence of e. histolytica to bhk monolayer was found to be mediated by n-acetyl-d-galactosamine. | 1998 | 9854433 |
| royal society of tropical medicine and hygiene meeting at manson house, london, 19 february 1998. amoebic disease. entamoeba dispar, an organism reborn. | entamoeba dispar was described by emile brumpt in 1925, and was promptly dismissed as a synonym of e. histolytica by almost all his contemporaries. in the 1970s, however, evidence began to accumulate indicating that brumpt may have been correct and e. dispar is now accepted as a distinct species. this review introduces the organism's history and briefly summarizes what we currently know about its biology. the acceptance of e. dispar has profound implications for our understanding of the epidemio ... | 1998 | 9850382 |
| the pyrophosphate-dependent phosphofructokinase of the protist, trichomonas vaginalis, and the evolutionary relationships of protist phosphofructokinases. | the pyrophosphate-dependent phosphofructokinase (ppi-pfk) of the amitochondriate protist trichomonas vaginalis has been purified. the enzyme is a homotetramer of about 50 kda subunits and is not subject to allosteric regulation. the protein was fragmented and a number of peptides were sequenced. based on this information a pcr product was obtained from t. vaginalis gdna and used to isolate corresponding cdna and gdna clones. southern analysis indicated the presence of five genes. one open readin ... | 1998 | 9847416 |
| [validation in havana city, cuba of enzymeba, an immunoassay for detecting entamoeba histolytica in feces]. | parasitologists from different medical institutions in havana city carried out the external validation of enzymeba, a diagnostic procedure of intestinal amebiasis developed at the "pedro kourí" institute of tropical medicine. to this end, serial faeces specimens from 212 individuals were collected and observed on the microscope (reference test). the enzymeba immunoassay (validation test) was also made. on comparing enzymeba with the microscopic examination, satisfactory indexes of sensitivity an ... | 1998 | 9842262 |
| prevalence and epidemiologic characteristics of opportunistic and non-opportunistic intestinal parasitic infections in hiv positive patients in manipur. | a survey of the intestinal parasites among the hiv positive asymptomatic injecting drug users (idus) in manipur state in north-east india revealed the presence of three protozoan and two nematode species. of these, the two opportunistic parasitic protozoans i.e., cryptosporidium sp. (94.4 percent) and isospora sp. (10.7 percent) were predominant over entamoeba histolytica (5.6 percent), while non-opportunistic parasitic nematodes, i.e. ascaris lumbricoides (4.6 percent) and ancylostoma sp. (1 pe ... | 1998 | 9842160 |
| mucosal and systemic suppression of the immune response to e. histolytica provoked by metronidazole and mebendazole in balb/c mice. | 1998 | 9836259 | |
| amoebic relaxant derived factor with activity on vascular smooth muscle. | 1998 | 9836255 | |
| case report: a patient who developed an amoebic liver abscess during treatment with interferon. | a 65-year-old female received recombinant interferon (ifn) alpha-2b daily for the treatment of chronic hepatitis c. fever (39 degrees c or higher) developed 14 days after the start of administration. abdominal computed tomography suggested multiple liver abscesses, which had not been detected before ifn administration. an autopsy revealed an amoebic liver abscess. a subclinical infection of entamoeba histolytica in this case developed into amoebic liver abscess during ifn administration. | 1998 | 9835326 |
| epithelial cell-initiated inflammation plays a crucial role in early tissue damage in amebic infection of human intestine. | entamoeba histolytica infection of the intestine can induce severe gut inflammation. the aims of this study were to assess the role of the host inflammatory response in the tissue damage observed with amebiasis and the role of the intestinal epithelial cell in initiating that response. | 1998 | 9834272 |
| host-pathogen interaction in amebiasis and progress in vaccine development. | entamoeba histolytica, the causative organism of invasive intestinal and extraintestinal amebiasis, infects approximately 50 million people each year, causing an estimated 40 to 100 thousand deaths annually. because amebae only infect humans and some higher non-human primates, an anti-amebic vaccine could theoretically eradicate the organism. uncontrolled epidemiologic studies indicate that acquired immunity to amebic infection probably occurs and that such a vaccine might be feasible. applicati ... | 1998 | 9832261 |
| henoch schönlein purpura and amebiasis. | the pathogenesis of henoch schönlein purpura (hsp) is unknown but is believed to result from an immune complex reaction to various antigenic stimuli, such as infectious agents. however, its association with entamoeba histolytica has not been reported before. we present an 11-year-old boy with hsp, confirmed by the demonstration of leukocytoclastic vasculitis from skin and diffuse endocapillary proliferative glomerulonephritis, together with immunoglobulin a and complement component c3 deposition ... | 1998 | 9821714 |
| parasites and diarrhea. i: protozoans and diarrhea. | intestinal parasitism is extremely common, with approximately 70% of all people harboring one or more intestinal parasite. parasitism and diarrhea are both hyperendemic in areas where sanitation is suboptimal. many clinicians assume that the identification of intestinal parasites in patients with diarrhea implies that the parasites are the cause. this approach is frequently misguided. some intestinal parasites such as giardia lamblia and entamoeba histolytica certainly do cause diarrhea. others, ... | 1997 | 9815473 |
| entamoeba histolytica: identification of functional gs and gi proteins as possible signal transduction elements in the interaction of trophozoites with fibronectin. | trophozoites of entamoeba histolytica adhere to several components of the extracellular matrix. binding is mediated by specific receptors identified in the parasite surface. interaction of trophozoites with fn induces the formation of special adhesion structures that are dynamic cytoskeleton membrane complexes and facilitate both adhesion and substrate degradation. the process requires activation of signaling pathways in which plc, ip3, ca2-, and pkc participate. these observations, and recent e ... | 1998 | 9806871 |
| molecular characterization of an exceptionally acidic lysozyme-like protein from the protozoon entamoeba histolytica. | the protozoan parasite entamoeba histolytica contains a second antibacterial protein with lysozyme-like properties. the newly recognized bacteriolytic protein was purified from extracts of amoebic trophozoites to allow amino-terminal sequencing. subsequent molecular cloning revealed that it is an isoform of the amoeba lysozyme described previously but also demonstrated a substantial sequence divergence of the two forms. as lysozymes typically are basic proteins, the novel amoebic protein differs ... | 1998 | 9804191 |
| secretory immune response in patients with intestinal amoebiasis. | the secretory immune response in saliva from intestinal amoebiasis patients against antigens obtained from entamoeba histolytica membranes was studied. western blot analysis indicated that patient saliva contains secretory iga antibodies against antigens with molecular masses ranging from 170 to 24 kda, some of which were also recognized by saliva from healthy subjects. however, antigens of 170, 125, 46 and 37 kda are recognized more frequently (> 90%) by the secretory iga from patients with int ... | 1998 | 9797512 |
| heat shock protein 70 family: multiple sequence comparisons, function, and evolution. | the heat shock protein 70 kda sequences (hsp70) are of great importance as molecular chaperones in protein folding and transport. they are abundant under conditions of cellular stress. they are highly conserved in all domains of life: archaea, eubacteria, eukaryotes, and organelles (mitochondria, chloroplasts). a multiple alignment of a large collection of these sequences was obtained employing our symmetric-iterative iteralign program (brocchieri and karlin 1998). assessments of conservation ar ... | 1998 | 9797407 |
| role of serum in regulating the entamoeba histolytica cell cycle: a flowcytometric analysis. | to identify stage-specific molecules that might be important in pathogenicity, we focused on synchronizing entamoeba histolytica hm1:1mss axenic cultures by serum deprivation. by growing trophozoites in medium containing 2% serum for 16 h and then replacing with complete medium, we found that most of the trophozoites were in the g0/g1 phase of the cell cycle. subsequently, the distribution of trophozoites in g0/g1, s and g2/m phases was studied for up to 24 h and it was observed that after 8 h, ... | 1998 | 9797069 |
| effect of bacterial association on the phenotype and genotype of an entamoeba histolytica clonal population. | a several-times-cloned population of entamoeba histolytica trophozoites (clone maviii) was cultured under axenic (maviiiax), monoxenic (maviiimx) and polyxenic (maviiipx) conditions. clones maviiiax and maviiimx presented similar virulence in vitro, but differed in their virulence in vivo, whereas maviiipx trophozoites were neither virulent in vitro or in vivo. the maviii clones maintained their zymodeme and exhibited three unusual glucose phosphate isomerase bands, absent in other e. histolytic ... | 1997 | 9778590 |
| involvement of the actin cytoskeleton and p21rho-family gtpases in the pathogenesis of the human protozoan parasite entamoeba histolytica. | it has been estimated that infection with the enteric protozoan parasite entamoeba histolytica kills more than 50,000 people a year. central to the pathogenesis of this organism is its ability to directly lyse host cells and cause tissue destruction. amebic lesions show evidence of cell lysis, tissue necrosis, and damage to the extracellular matrix. the specific molecular mechanisms by which these events are initiated, transmitted, and effected are just beginning to be uncovered. in this article ... | 1998 | 9777011 |
| some gastro-intestinal parasites of zoonotic (public health) importance commonly observed in old world non-human primates in kenya. | a study was undertaken to categorise some gastro-intestinal (git) parasites commonly observed in kenyan non-human primates (nhps) on the basis of their health implications for humans. six species of locally available non-human primates, namely olive baboons (papio cyanocephalus anubis), vervet monkey (cercopithecus aethiops), sykes monkey (cercopithecus mitis), black and white colobus (colobus abyssinicus), debrazzas monkey (cercopithecus neglectus) and grey and black mangabeys (cercocebus torqu ... | 1998 | 9776144 |
| intestinal amoebiasis: antibody-secreting cells and humoral antibodies. | splenic plasma cell response and systemic antibody response to intestinal amoebiasis were studied in c3h/hej mice from 5 to 60 days post-inoculation with entamoeba histolytica. at various time intervals specific antibody-secreting cells (asc) in the spleen were measured in infected mice and non-infected control mice by enzyme-linked immunospot (elispot) assay. serum antibodies were measured by enzyme-linked immunosorbent assay (elisa). the infected animals showed high iga asc from 30 to 50 days ... | 1998 | 9775574 |
| diagnosis of trichomonas vaginalis infection by pcr using vaginal swab samples. | trichomonas vaginalis infection is the most prevalent nonviral sexually transmitted disease (std) in the world. a pcr test using vaginal swab samples for the detection of t. vaginalis was developed to add t. vaginalis infection to the growing list of stds that can be detected by dna amplification techniques. a primer set, btub 9/2, was designed to target a well-conserved region in the beta-tubulin genes of t. vaginalis. all strains (15 of 15) of t. vaginalis tested were successfully detected by ... | 1998 | 9774566 |
| simultaneous amoebic liver abscess and hepatitis a infection. | hepatitis a virus (hav) infection and amoebic liver abscess have an identical mode of transmission (fecal-oral route) and similar epidemiology. either of these diseases might be considered in patients presenting with clinical signs and symptoms of liver disease in areas of the world where hepatitis and entamoeba histolytica are endemic. the following two cases describe patients with simultaneous infection of hav and amoebic liver abscess. this report emphasizes the role of dual infection in pati ... | 1998 | 9772324 |
| identification and characterisation of early reactions of asparagine-linked oligosaccharide assembly in entamoeba histolytica. | sequential incubation of a mixed membrane fraction isolated from entamoeba histolytica trophozoites with the nonionic detergents brij 35 and igepal ca-630 rendered a soluble fraction with the ability to transfer n-acetylglucosamine (glcnac) from udp-glcnac to dolichol phosphate to form a lipid saccharide that was identified as a mixture of dolichol-p-p-glcnac and dolichol-p-p-(glcnac)2 as follows. (a) the reaction occurred only in the presence of exogenously added dolichol phosphate and was stro ... | 1998 | 9770617 |
| [fasciola hepatica antigens. use in the diagnosis of human fascioliasis]. | the elisa technique was standardized for detecting antibodies against fasciola in 25 serum samples from patients-infected with fasciola hepatica using excretion-secretion antigens (esa), tegument antigen and somatic antigen. a study was made of serum samples from 46 patients with other parasites such as ascaris lumbricoides, trichuris trichiura, entamoeba histolytica, schistosoma mansoni and giardia lamblia. the cohort value for each trial was established after studying 100 serum samples from he ... | 1991 | 9768179 |
| [amebiasis--a differential diagnosis from inflammatory bowel disease]. | entamoeba histolytica is a very uncommon etiology of travelers diarrhoea, but may be an important differential diagnosis to inflammatory bowel disease. a misdiagnosis of amoebic colitis as inflammatory bowel disease followed by inadvertent treatment with corticosteroids may be fatal. current techniques for the diagnosis of amoebiasis include stool studies, endoscopic biopsy and serology. demonstration of haematophagous trophozoites in feces or in tissue biopsy is the definitive method for diagno ... | 1998 | 9763927 |
| [intestinal parasitism in a parakanã indigenous community in southwestern pará state, brazil]. | to determine the occurrence and epidemiological aspects of intestinal parasites among the parakanã indigenous people in the paranatinga settlement (in the eastern amazon region), parasitological tests were performed in april 1992 and february 1995. one fresh stool specimen was obtained and immediately processed using the hoffman and direct methods. some 126 samples were obtained in april 1992 (from a total population of 215 individuals). some 80. 2% (101) of those tested were infected with at le ... | 1998 | 9761604 |
| helminth and protozoan gastrointestinal tract parasites in captive and wild-trapped african non-human primates. | the objective of this study was to investigate the gastro-intestinal (git) parasites commonly occurring in captive and wild-trapped (wt) non-human primates (baboons, vervets and sykes) in kenya and compare their prevalence. three hundred and fifteen faecal samples were subjected to a battery of diagnostic tests, namely, direct smear, modified formal ether sedimentation, kato thick smear, harada-mori techniques for parasite detection and culture to facilitate nematode larvae identification. of th ... | 1998 | 9760061 |
| schistosoma mansoni, intestinal parasites and perceived morbidity indicators in schoolchildren in a rural endemic area of western côte d'ivoire. | there is a great need for rapid and low-cost identification of communities at high risk of intestinal schistosomiasis. we report the development of a questionnaire approach that may do so. in the first phase, 209 schoolchildren from 3 neighbouring villages in a rural area endemic for intestinal schistosomiasis in western côte d'ivoire were screened for schistosoma mansoni and other helminths on 4 consecutive days using kato-katz thick smears. daily infection prevalences of s. mansoni were high ( ... | 1998 | 9754666 |
| [cytoskeletal actin and its associated proteins. some examples in protista]. | many processes, cell motility being an example, require cells to remodel the actin cytoskeleton in response to both intracellular and extracellular signals. reorganization of the actin cytoskeleton involves the rapid disassembly and reassembly of actin filaments, a phenomenon regulated by the action of particular actin-binding proteins. in recent years, an interest in studying actin regulation in unicellular organisms has arisen. parasitic protozoan are among these organisms and studies of the c ... | 1998 | 9754306 |
| an essential methionine residue involved in substrate binding by phosphofructokinases. | an alignment of all ppi-dependent phosphofructokinases and all allosteric atp-dependent pfks shows relatively few residues that are fully conserved. one residue that is conserved is a methionine residue that appears from the crystal structure of escherichia coli pfk to be interacting with fructose 6-p. very conservative substitutions for this methionine with leucine or isoleucine by site-directed mutagenesis of e. coli atp-pfk and entamoeba histolytica ppi-pfk produced profound decreases either ... | 1998 | 9753654 |
| [a case of intestinal amebiasis]. | 1998 | 9750051 | |
| serum igm antibody response to the galactose-inhibitable adherence lectin of entameoba histolytica. | an elisa for detection of serum igm antibodies to the galactose-inhibitable adherence lectin of entamoeba histolytica revealed that 2.8% of uninfected controls, 0.0% of controls infected with other parasites, 13.4% of asymptomatic amebic infections, 55% of colitis patients, and 77% of amebic liver abscess patients from cairo, egypt and durban, south africa had serum anti-lectin igm antibodies. of acute amebic colitis patients with symptoms for less than one week, only 6% possessed serum igg anti ... | 1998 | 9749639 |
| development of monoclonal antibodies specifically recognizing the cyst stage of entamoeba histolytica. | protozoan cysts were isolated according to a two-step sucrose gradient procedure. pure cysts of entamoeba histolytica, in fixed and native states, were injected into balb/c mice intraperitoneally for immunization. the spleens of these animals were used for fusion with ag8 mouse myeloma cells. hybridomas were obtained and tested for the recognition of e. histolytica, e. dispar, e. coli, e. hartmanni, endolimax nana, jodamoeba biitschlii, and giardia lamblia. three monoclonal antibodies were ident ... | 1998 | 9749623 |
| an upstream regulatory element containing two nine basepair repeats regulates expression of the entamoeba histolytica hgl5 lectin gene. | 1998 | 9747982 | |
| identification of the 150-kda surface antigen of entamoeba histolytica as a galactose- and n-acetyl-d-galactosamine-inhibitable lectin. | a monoclonal antibody that reacts with a 150-kda protein of entamoeba histolytica on western immunoblotting under nonreducing conditions inhibits the adherence and cytotoxicity of the ameba to mammalian cells in vitro. affinity purification of solubilized trophozoites using the monoclonal antibody and electrophoresis yielded three glycoproteins with molecular masses of 150, 170, and 260 kda, suggesting the existence of either a common epitope or the close association of these proteins. the 260-k ... | 1998 | 9747935 |
| prevalence of intestinal parasites among children in southern sudan. | a study was conducted in southern sudan to determine the prevalence of intestinal parasites among school children. a total of 275 stool samples which were examined using formol-ether concentration techniques yielded 15 different species of parasites. hook worm with a prevalence of 13.1% was the predominant nematode followed by strongyloides stercoralis (3.3%), trichostrongylus (2.5%), schistosoma mansoni (2.2%) and trichuris trichiura (1.8%). ascaris lumbricoides and cestodes were not detected i ... | 1998 | 9747000 |
| hepatic amebiasis among patients in a public teaching hospital. | the increase in immigration to the united states is associated with diseases, such as amebiasis, that are endemic to developing countries. we retrospectively reviewed 49 public-hospital patients with hepatic amebiasis occurring between 1985 and 1995. most patients were immigrants (47) from latin america (43), male (43), and young (mean age, 39.8 years). symptoms noted by more than half were abdominal pain and fever. ultrasonography showed single lesions in 70% and right-sided involvement in 85%. ... | 1998 | 9743053 |
| seropositivity for and intestinal colonization with entamoeba histolytica and entamoeba dispar in individuals in northeastern brazil. | in a slum community in northeastern brazil 20% of a sample population was colonized with entamoeba histolytica or entamoeba dispar and 10.6% was colonized with e. histolytica alone. no correlation between seropositivity for anti-ga1nac lectin antibody and colonization was found. these results suggest that colonization does not necessarily produce immunity to reinfection. | 1998 | 9738064 |
| disparate amoebae. | 1998 | 9734879 | |
| pna molecular beacons for rapid detection of pcr amplicons. | the authors have developed a method for rapid detection of polymerase chain reaction (pcr) amplicons based on surface immobilized pna-dna hybrid probes ('molecular beacons') that undergo a fluorescent-linked conformational change in the presence of a complementary dna target. amplicons can be detected by simply adding a pcr reaction to a microtitre-well containing the previously immobilized probe, and reading the generated fluorescence. no further transfers or washing steps are involved. the aut ... | 1998 | 9727198 |
| stool culture as a diagnostic aid in the detection of entamoeba histolytica in the faecal specimens. | "the use of stool culture in boeck & drbohlav's biphasic amoebic medium as a routine diagnostic aid in the detection of entamoeba histolytica in the faeces, is evaluated in the present study. a total of 3803 faecal specimens were examined for the presence of e.histolytica by direct smear, formalin ether concentration and culture during a study period of 1982-1990. a total of 259 stool specimens were positive for the parasite by any or all of these methods 42 (16.21%) stool specimen not cultured ... | 1995 | 9726144 |
| substructural specificity and polyvalent carbohydrate recognition by the entamoeba histolytica and rat hepatic n-acetylgalactosamine/galactose lectins. | both the entamoeba histolytica lectin, a virulence factor for the causative agent of amebiasis, and the mammalian hepatic lectin bind to n-acetylgalactosamine (galnac) and galactose (gal) nonreducing termini on oligosaccharides, with preference for galnac. polyvalent galnac-derivatized neoglycoproteins have >1000-fold enhanced binding affinity for both lectins (adler,p., wood,s.j., lee,y.c., lee,r.t., petri,w.a.,jr. and schnaar,r.l.,1995, j. biol. chem ., 270, 5164-5171). substructural specifici ... | 1998 | 9719685 |
| effects of aphidicolin on entamoeba histolytica growth and dna synthesis. | we have previously demonstrated that dna polymerase activity of entamoeba histolytica is inhibited by aphidicolin, which is a specific inhibitor of eukaryotic nuclear replicative dna polymerases. the present study was aimed to evaluate the effect of aphidicolin on growth and dna synthesis by this parasite. aphidicolin blocked the growth of axenic e. histolytica strain hm-1:imss. dna synthesis was also inhibited by aphidicolin when assayed by incorporation of [3h]thymidine into the dna. the inhib ... | 1998 | 9714225 |
| the novel core promoter element gaac in the hgl5 gene of entamoeba histolytica is able to direct a transcription start site independent of tata or initiator regions. | entamoeba histolytica, an enteric protozoa, is the third leading parasitic cause of death worldwide. investigation of the transcriptional machinery of this eukaryotic pathogen has revealed an unusual core promoter structure that consists of nonconsensus tata and initiator regions and a novel third conserved core promoter sequence, the gaac element. mutation of this region in the hgl5 promoter decreases reporter gene expression and alters the transcription start site. using positional analysis of ... | 1998 | 9705300 |
| bacterium-assisted invasion of entamoeba histolytica through human enteric epithelia in two-compartment chambers. | entamoeba histolytica trophozoites initiate amebiasis by invasion into the enteric mucosa. it is the aim of our experiments to understand how bacteria and leukocytes act during amebic invasion through enteric cell layers. cocultures were established in two-compartment chambers and studied by measurement of transepithelial electrical resistance (ter) and by histological examination. trophozoites caused a decrease in ter that was followed by formation of holes in the enteric cell layer and transfi ... | 1997 | 9702940 |
| does the eosinophil have a protective role in amebiasis? | while normal human eosinophils are destroyed in vitro by virulent entamoeba histolytica, notwithstanding the presence of antibodies and complement, activated eosinophils promptly destroy the parasite although dying also at the end of the process. to study the possible in vivo participation of eosinophils in invasive amebiasis, we compared the induction of experimental amebic abscess of the liver (aal) in gerbils (meriones unguiculatus) previously made eosinophilic through toxocara canis antigen ... | 1997 | 9698942 |
| regulation of adherence and virulence by the entamoeba histolytica lectin cytoplasmic domain, which contains a beta2 integrin motif. | killing of human cells by the parasite entamoeba histolytica requires adherence via an amebic cell surface lectin. lectin activity in the parasite is regulated by inside-out signaling. the lectin cytoplasmic domain has sequence identity with a region of the beta2 integrin cytoplasmic tail implicated in regulation of integrin-mediated adhesion. intracellular expression of a fusion protein containing the cytoplasmic domain of the lectin has a dominant negative effect on extracellular lectin-mediat ... | 1998 | 9693367 |
| antiamoebic and phytochemical screening of some congolese medicinal plants. | results from the in vitro antiamoebic activity of some congolese plant extracts used as antidiarrhoeic in traditional medicine indicated that of 45 plant extracts tested, 35 (77.78%) exhibited an antiamoebic activity and 10 (22.22%) were inactive. the highest activity (mic < 100 microg/ml) was obtained with extracts from root bark of paropsia brazzeana, cryptolepis sanguinolenta, alchornea cordifolia, hensia pulchella, maprounea africana, rauwolfia obscura and voacanga africana, leaves and stem ... | 1998 | 9687082 |
| entamoeba histolytica and entamoeba dispar in the northeast brazil. | 1997 | 9674291 | |
| application of lactophenol cotton blue for identification and preservation of intestinal parasites in faecal wet mounts. | wet mounting is the simplest and easiest technique for the examination of faeces. routinely employed wet mounts in most of the laboratories include saline, iodine and buffered methylene blue. lactophenol cotton blue [lcb] staining--mounting medium is commonly used for microscopic identification of fungi. lcb and its modifications were tested as staining mounting medium on parasite positive faecal samples [both fresh as well as formalin fixed] to stain and demonstrate morphology and to preserve p ... | 1998 | 9670627 |
| ecto-nucleotide triphosphatase activity in pathogenic and non-pathogenic entamoeba: protection from the cytotoxic effects of extracellular atp. | the nucleotide triphosphatase (ntpase) activity of entamoeba species and of entamoeba histolytica strains, was compared. in all cases, with the exception of entamoeba moshkovskii, the enzyme was activated by ca2+ and not by mg2+ and preferentially hydrolysed utp with decreasing activity for atp and gtp. the ntpase activity was associated with both the intracellular and the plasma membrane sulphonylbenzoyl-adenosine (fsba) of trophozoites in which the atp-site was facing externally, as shown by f ... | 1997 | 9670549 |
| entamoeba dispar: ultrastructure, surface properties and cytopathic effect. | the cytological features of entamoeba dispar, recently recognized by biochemical and molecular biology criteria as a distinct species, were compared to those of entamoeba histolytica when cultured under axenic conditions, living trophozoites of e. dispar strain saw 76orr clone a were more elongated in form, had a single frontal pseudopodium, and showed a noticeable uroid. in sections of e. dispar trophozoites stained with toluidine blue, characteristic areas of cytoplasmic metachromasia were see ... | 1998 | 9669863 |
| the human monocyte locomotion-inhibitory factor produced by entamoeba histolytica does not inhibit the locomotion of human eosinophils. | 1998 | 9660147 | |
| synergy between entamoeba histolytica and escherichia coli in the induction of cytokine gene expression in human colon epithelial cells. | entamoeba histolytica induces the expression and secretion of interleukin 8 (il-8) by cultured colon epithelial cells. we assessed the array of cytokine genes expressed by human colon epithelial cells in response to co-culture with e. histolytica trophozoites and tested the hypothesis that enteric bacteria may alter the e. histolytica-induced expression of such genes. ht-29 colon epithelial cells were co-cultured with e. histolytica trophozoites in the presence or absence of escherichia coli. cy ... | 1998 | 9660143 |
| cloning and expression of the entamoeba histolytica erd2 gene. | 1998 | 9657339 | |
| role of neutrophils in innate resistance to entamoeba histolytica liver infection in mice. | in order to define the role of neutrophils in the innate resistance to entamoeba histolytica liver infection in mice, we examined the pattern of liver lesion induced by direct injection of e. histolytica trophzoites in normal mice and in neutrophil-depleted mice. a variety of histological lesions were found, the extent of liver damage was considerably higher in the neutrophil-depleted mice. livers from neutrophil-depleted mice displayed areas of liquefactive (lytic) necrosis containing a large n ... | 1998 | 9651927 |
| evaluation of intestinal protozoan morphology in human fecal specimens preserved in ecofix: comparison of wheatley's trichrome stain and ecostain. | as a result of disposal problems related to the use of mercury compounds, many laboratories have switched from mercuric chloride-based schaudinn's and polyvinyl alcohol (pva) stool preservatives to other, non-mercury-based preservatives. a comparison of organism recoveries and morphologies of the intestinal protozoa was undertaken with pva containing the ecofix zinc-based schaudinn's preservative (meridian diagnostics, inc.); both wheatley's modification of gomori's trichrome stain (wt) and ecos ... | 1998 | 9650947 |
| axenic cultivation of trichomonas vaginalis in a serum-free medium. | mammalian serum or bovine serum albumin are essential for trichomonas vaginalis cultivated under axenic conditions. unfortunately, these components inhibit several biological properties of these parasites. pacsr is a serum replacement, free of bovine serum albumin. used for axenic cultivation of entamoeba histolytica. we show that pacsr is also useful for axenic cultivation of t. vaginalis. tubes containing 5.5 ml pehp, or tyi basal media, plus 8% pacsr (v/v), were inoculated with 10(3) trichomo ... | 1998 | 9645876 |
| intestinal parasite infections after extended use of chloroquine or primaquine for malaria prevention. | comparative results of baseline and endpoint screening for intestinal parasites are reported from javanese men enrolled in a year-long, placebo-controlled malaria prophylaxis trial in irian jaya. the objective was to detect nontarget qualitative changes that may have resulted from prolonged chloroquine (300 mg base weekly) or primaquine (0.5 mg base/kg daily) prophylaxis. fresh fecal specimens were examined (blinded trial) for parasites and ova using a modified kato-katz thick smear method. more ... | 1998 | 9645872 |