Publications

TitleAbstractYear
Filter
PMID(sorted descending)
Filter
the wheat gene tast can increase the salt tolerance of transgenic arabidopsis.on the basis of the results of gene chip analysis of the salt-tolerant wheat mutant rh8706-49 under conditions of salt stress, we identified and cloned an unknown salt-induced gene tast (triticum aestivum salt-tolerant). real-time quantitative pcr analysis showed that the expression of the gene was induced by salt stress. transgenic arabidopsis plants overexpressing the tast gene showed higher salt tolerance than the wild-type controls. subcellular localization studies revealed that the protein ...201221993814
Graminan breakdown by fructan exohydrolase induced in winter wheat inoculated with snow mold.Fructan structures vary widely among plant species. Graminan-type fructans, extensions of sucrose through ß-(2,6)-linked fructosyl units with branches of ß-(2,1)-linked fructosyl units, accumulate in tissues of winter wheat (Triticum aestivum) during cold hardening and are metabolized under persistent snow cover. Snow molds such as Typhula ishikariensis and Microdochium nivale opportunistically infect wheat under snow cover. Snow mold-resistant wheat cultivars tend to heavily accumulate and slow ...201121983139
the effect of cultivation intensity on mineral content in grain, flakes and bran of winter wheat (triticum aestivum l.)--preliminary study.the aim of the study was to assess the effect of cultivation intensity on the ca, mg, zn and fe contents in winter wheat grain, flakes and bran. the initial material for the study comprised grain, flakes and bran of two winter wheat cultivars kobiera and bogatka. these cultivars were from two strain testing stations located in lower silesia, zybiszów and tomaszów bolesławiecki in the years 2007-2008. the cultivation was conducted on two intensity levels: lower and higher the mineral content was ...201121980867
synthetic antisense oligodeoxynucleotides to transiently suppress different nucleus- and chloroplast-encoded proteins of higher plant chloroplasts.selective inhibition of gene expression by antisense oligodeoxynucleotides (odns) is widely applied in gene function analyses; however, experiments with odns in plants are scarce. in this work, we extend the use of odns in different plant species, optimizing the uptake, stability, and efficiency of odns with a combination of molecular biological and biophysical techniques to transiently inhibit the gene expression of different chloroplast proteins. we targeted the nucleus-encoded phytoene desatu ...201121980174
salicylic acid and calcium-induced protection of wheat against salinity.soil salinity is one of the important environmental factors that produce serious agricultural problems. the objective of the present study was to determine the interactive effect of salicylic acid (sa) and calcium (ca) on plant growth, photosynthetic pigments, proline (pro) concentration, carbonic anhydrase (ca) activity and activities of antioxidant enzymes of triticum aestivum l. (cv. samma) under salt stress. application of 90 mm of nacl reduced plant growth (plant height, fresh weight (fw) a ...201121979309
Next-generation sequencing and syntenic integration of flow-sorted arms of wheat chromosome 4A exposes the chromosome structure and gene content.Wheat is the third most important crop for human nutrition in the world. The availability of high-resolution genetic and physical maps and ultimately a complete genome sequence holds great promise for breeding improved varieties to cope with increasing food demand under the conditions of changing global climate. However, the large size of the bread wheat (Triticum aestivum) genome (approximately 17 Gb/1C) and the triplication of genic sequence resulting from its hexaploid status have impeded gen ...201121974774
genomic diversity of ac-like transposable elements in sphaerococcum mutant forms of common wheat (triticum aestivum l.) and triticale (x triticosecale witt.).dna sequences homologous to the maize activator (ac) element are widespread in plant genomes. nowadays, several reports are available concerning the distribution and characterisation of ac-homologous sequences in natural populations of different cereal species. but these mobile genetic elements still remain to be comprehensively characterised. in this respect, there is a particular lack of information about the dynamics of ac-homologous sequences within mutant germplasm collections. here, we pre ...201221971991
Impact of Triticum mosaic virus infection on hard winter wheat milling and bread baking quality.BACKGROUND: Triticum mosaic virus (TriMV) is a newly discovered wheat virus. Information regarding the effect of wheat viruses on milling and baking quality is limited. The objective of this study was to determine the impact of TriMV infection on the kernel characteristics, milling yield and bread baking quality of wheat. Commercial hard winter varieties evaluated included RonL, Danby and Jagalene. The TriMV resistance of RonL is low, while that of Danby and Jagalene is unknown. KS96HW10-3, a ge ...201121969097
comparison of photosynthetic components of wheat genotypes under rain-fed and irrigated conditions.the major environmental factor limiting the range of adaptation for wheat is drought. fourteen wheat genotypes (triticum aestivum l.) were grown under two environments (irrigated and rain fed) to determine physiological and photosynthetic responses to drought. combined analysis of variance of the data showed that the environment was a significant source of variation for leaf chlorophyll content (lcc), stomatal conductance (g(s)) and grain yield (gy). wheat genotypes differed significantly for lc ...201221967636
development of a set of compensating triticum aestivum-dasypyrum villosum robertsonian translocation lines.dasypyrum villosum (l.) candargy, a wild relative of bread wheat ( triticum aestivum l.), is the source of many agronomically important genes for wheat improvement. production of compensating robertsonian translocations (crobts), consisting of d. villosum chromosome arms translocated to homoeologous wheat chromosome arms, is one of the initial steps in exploiting this variation. the crobts for d. villosum chromosomes 1v, 4v, and 6v have been reported previously. here we report attempted crobts f ...201121961939
identification of genetic factors controlling kernel hardness and related traits in a recombinant inbred population derived from a soft × 'extra-soft' wheat (triticum aestivum l.) cross.kernel hardness or texture, used to classify wheat (triticum aestivum l.) into soft and hard classes, is a major determinant of milling and baking quality. wheat genotypes in the soft class that are termed 'extra-soft' (with kernel hardness in the lower end of the spectrum) have been associated with superior end-use quality. in order to better understand the relationship between kernel hardness, milling yield, and various agronomic traits, we performed quantitative trait mapping using a recombin ...201221959906
mapping and validation of quantitative trait loci associated with wheat yellow mosaic bymovirus resistance in bread wheat.wheat yellow mosaic (wym) caused by wheat yellow mosaic bymovirus (wymv) has been growing as one of the most serious diseases affecting wheat production in china. in this study, the association of quantitative trait loci (qtls) governing wymv resistance with molecular markers was established using 164 recombinant inbred lines (rils) derived from 'xifeng wheat' (highly resistant) × 'zhen 9523' (highly susceptible). phenotypic data of wymv resistance of the rils were collected from 4-year, two-loc ...201221959905
changes in wheat leaf phenolome in response to cold acclimation.a study of wheat (triticum aestivum l.) leaves phenolome was carried out during cold acclimation of the winter (claire) and spring (bounty) varieties using a combination of hplc-esi-ms techniques. a total of 40 phenolic and flavonoid compounds were identified, and consisted mainly of two coumarin derivatives, eight simple phenolic derivatives, 10 hydroxycinnamoyl amides and 20 flavonoid derivatives. identification and quantification of individual compounds were performed using an hplc system cou ...201121955620
[preferential elimination of chromosome 5r of rye in the progeny of 5r5d dimonosomics].transmission of chromosome 5r of rye (secale cereale l.) and chromosome 5d of common wheat (triticum aestivum l.) through gametes of 5r5d dimonosomics (2n = 42, 20w" + 5r' + 5d') was studied. chromosome 5r was found to have lower competitiveness as compared to 5d. gametes with the rye chromosome were two times less often involved in the formation of a progeny. the combined frequency of the karyotypes of wheat (5d5d) and wheat monosomics (5d) was 11.6-fold higher than the frequency of the karyoty ...201121954615
the agrobacterium-mediated transformation of common wheat (triticum aestivum l.) and triticale (x triticosecale wittmack): role of the binary vector system and selection cassettes.the influence of two binary vector systems, pgreen and pcambia, on the agrobacterium-mediated transformation ability of wheat and triticale was studied. both vectors carried selection cassettes with bar or nptii driven by different promoters. two cultivars of wheat, kontesa and torka, and one cultivar of triticale, wanad, were tested. the transformation rates for the wheat cultivars ranged from 0.00 to 3.58% and from 0.00 to 6.79% for triticale. the best values for wheat were 3.58% for kontesa a ...201121952729
identification of a large deletion in the first intron of the vrn-d1 locus, associated with loss of vernalization requirement in wild wheat progenitor aegilops tauschii coss.vernalization promotes flowering in winter wheat cultivars, whereas spring wheat cultivars are able to transition from vegetative to reproductive phase without vernalization. the wheat vernalization requirement is mainly controlled by the major locus vrn-1, an apetala1/fruitfull mads-box gene homolog. to study natural variation of the vernalization requirement in a wild progenitor of common wheat, we sequenced the vrn-d(t)1 locus in four accessions of aegilops tauschii coss. some structural muta ...201121952208
reference genes for gene expression studies in wheat flag leaves grown under different farming conditions.internal control genes with highly uniform expression throughout the experimental conditions are required for accurate gene expression analysis as no universal reference genes exists. in this study, the expression stability of 24 candidate genes from triticum aestivum cv. cubus flag leaves grown under organic and conventional farming systems was evaluated in two locations in order to select suitable genes that can be used for normalization of real-time quantitative reverse-transcription pcr (rt- ...201121951810
a new insight into application for barley chromosome addition lines of common wheat: achievement of stigmasterol accumulation.barley (hordeum vulgare) has a much higher content of bioactive substances than wheat (triticum aestivum). in order to investigate additive and/or synergistic effect(s) on the phytosterol content of barley chromosomes, we used a series of barley chromosome addition lines of common wheat that were produced by normal crossing. in determining the plant sterol levels in 2-week-old seedlings and dry seeds, we found that the level of stigmasterol in the barley chromosome 3 addition (3h) line in the se ...201121951468
[the chiral mutagens: cytogenetic effects on higher plants].the paper covers investigation of cytogenetic activity of chiral mutagens and their specific effects on the plant cells chromosomes of soft winter wheat (triticum aestivum l.). comparative analysis of cytogenetic activity of chiral neu: s(+)1-n-nitroso- 1-n-methyl-3-n-sec-buthylureas (s(+)nmsbu) and r(-)1-n-nitroso- 1n-methyl-3-nsec-buthylureas (r(-)nmsbu) on winter wheat was performed. as it was shown by the frequency of chromosomal aberrations the s(+) stereoisomer was twice more active than r ...201121950141
[genoprotective activities of the oils from leaves and fruits of fagus orientalis lipsky].the antimutagenic activities of the oils obtained from leaves and fruits of fagus orientalis have been shown in experiments with spontaneous and mutagen- and ageing-induced variability. the aberrations of chromosomes in the meristematic cells of the allium cepa l., vicia faba l., triticum aestivum l., and marrow cells of vistar rats as well as arabidopsis thaliana gene mutations have been mobilized as experimental tests.201121950139
characterization of chloroplast dna microsatellites from saccharum spp and related species.microsatellites, or simple sequence repeats (ssrs), and their flanking regions in chloroplast genomes (plastomes) of some species of the family poaceae were analyzed in silico to look for dna sequence variations. comparison of the complete chloroplast dna sequences (cpdnas) of sugarcane (saccharum hybrid cv. sp-80-3280 and s. officinarum cv. nco310) and related species, agrostis stolonifera, brachypodium distachyon, hordeum vulgare subsp vulgare, lolium perenne, oryza nivara, o. sativa sub ...201121948764
Cloning, Escherichia coli expression, purification, characterization, and enzyme assay of the ribosomal protein S4 from wheat seedlings (Triticum vulgare).S4 is a paradigm of ribosomal proteins involved in multifarious activities both within and outside the ribosome. For a detailed biochemical and structural investigations of eukaryotic S4, the wheat S4 gene has been cloned and expressed in Escherichia coli, and the protein purified to a high degree of homogeneity. The 285-residue recombinant protein containing an N-terminal His(6) tag along with fourteen additional residues derived from the cloning vector is characterized by a molecular mass of 3 ...201221945701
[Effects of reduced solar radiation on winter wheat flag leaf net photosynthetic rate].Taking winter wheat Triticum aestivum L. (cv. Yangmai 13) as test material, a field experiment was conducted in Nanjing City to study the effects of simulated reduced solar radiation on the diurnal variation of winter wheat flag leaf photosynthetic rate and the main affecting factors. Five treatments were installed, i. e., 15% (T15), 20% (T20) , 40% (T40), 60% (T60), and 100% (CK) of total incident solar radiation. Reduced solar irradiance increased the chlorophyll and lutein contents significan ...201121941745
[analysis of diversity of russian and ukrainian bread wheat (triticum aestivum l.) cultivars for high-molecular-weight glutenin subunits].the allelic diversity of high-moleculat-weght glutenin subunits (h wigs) in russian and ukrainian bread wheat cultivars was analyzed. the diversity of spring wheat cultivars for alleles of the glu-1 loci is characterized by medium values of the polymorphism index (polymorphism information content, plc), and in winter wheats it varies from high at the glu-a1 locus to low at the glu-d1 locus. the spring and winter cultivars differ significantly in the frequencies of alleles of the glutenin loci. t ...201121938954
A second 'overexpression' allele at the Glu-B1 high-molecular-weight glutenin locus of wheat: sequence characterisation and functional effects.Bread is one of the major constituents of the human diet and wheat (Triticum aestivum L.) is the most important cereal for bread making. The gluten proteins (glutenins and gliadins) are recognised as important components affecting the processing quality of wheat flour. In this research, we investigated a particular glutenin subunit allele in an Australian cultivar, H45. Based on protein and DNA assays, the Glu-B1 allele of H45 seems to be Glu-B1al, an allele that includes a functional duplicatio ...201121938472
down-regulating γ-gliadins in bread wheat leads to non-specific increases in other gluten proteins and has no major effect on dough gluten strength.gliadins are a major component of gluten proteins but their role in the mixing of dough is not well understood because their contribution to wheat flour functional properties are not as clear as for the glutenin fraction.201121935456
genetic control of duration of pre-anthesis phases in wheat (triticum aestivum l.) and relationships to leaf appearance, tillering, and dry matter accumulation.the duration of pre-anthesis developmental phases is of interest in breeding for improved adaptation and yield potential in temperate cereals. yet despite numerous studies on the genetic control of anthesis (flowering) time and floral initiation, little is known about the genetic control of other pre-anthesis phases. furthermore, little is known about the effect that changes in the duration of pre-anthesis phases could have on traits related to leaf appearance and tillering, or dry matter accumu ...021920907
Genetic analysis of adult plant, quantitative resistance to stripe rust in wheat cultivar 'Stephens' in multi-environment trials.The wheat (Triticum aestivum L.) cultivar 'Stephens' has been grown commercially in the USA Pacific Northwest for 30 years. The durable resistance of 'Stephens' to stripe rust (Puccinia striiformis f. sp. tritici) was believed to be due to a combination of seedling and adult plant resistance genes. Multilocation field trials, diversity array technology (DArT), and simple sequence repeat (SSR) markers were used to identify quantitative trait loci (QTL) for resistance. Recombinant inbred lines wer ...201121912857
new broad-spectrum resistance to septoria tritici blotch derived from synthetic hexaploid wheat.septoria tritici blotch (stb), caused by the ascomycete mycosphaerella graminicola, is one of the most devastating foliar diseases of wheat. we screened five synthetic hexaploid wheats (shs), 13 wheat varieties that represent the differential set of cultivars and two susceptible checks with a global set of 20 isolates and discovered exceptionally broad stb resistance in shs. subsequent development and analyses of recombinant inbred lines (rils) from a cross between the sh m3 and the highly susce ...201121912855
A wheat homolog of MOTHER OF FT AND TFL1 acts in the regulation of germination.Seed dormancy is an adaptive mechanism and an important agronomic trait. Temperature during seed development strongly affects seed dormancy in wheat (Triticum aestivum) with lower temperatures producing higher levels of seed dormancy. To identify genes important for seed dormancy, we used a wheat microarray to analyze gene expression in embryos from mature seeds grown at lower and higher temperatures. We found that a wheat homolog of MOTHER OF FT AND TFL1 (MFT) was upregulated after physiologica ...201121896881
functional features of a single chromosome arm in wheat (1al) determined from its structure.bread wheat (triticum aestivum l.) is one of the most important crops globally and a high priority for genetic improvement, but its large and complex genome has been seen as intractable to whole genome sequencing. isolation of individual wheat chromosome arms has facilitated large-scale sequence analyses. however, so far there is no such survey of sequences from the a genome of wheat. greater understanding of an a chromosome could facilitate wheat improvement and future sequencing of the entire ...201221892730
characterization of nitrogen-fixing bacteria isolated from field-grown barley, oat, and wheat.diazotrophic bacteria were isolated from the rhizosphere of field-grown triticum aestivum, hordeum vulgare, and avena sativa grown in various regions of greece. one isolate, with the highest nitrogen-fixation ability from each of the eleven rhizospheres, was selected for further characterisation. diazotrophic strains were assessed for plant-growth-promoting traits such as indoleacetic acid production and phosphate solubilisation. the phylogenies of 16s rrna gene of the selected isolates were com ...201121887633
the effects of phenotypic plasticity on photosynthetic performance in winter rye, winter wheat and brassica napus.the contributions of phenotypic plasticity to photosynthetic performance in winter (cv musketeer, cv norstar) and spring (cv sr4a, cv katepwa) rye (secale cereale) and wheat (triticum aestivum) cultivars grown at either 20°c [non-acclimated (na)] or 5°c [cold acclimated (ca)] were assessed. the 22-40% increase in light-saturated rates of co₂ assimilation in ca vs na winter cereals were accounted for by phenotypic plasticity as indicated by the dwarf phenotype and increased specific leaf weight. ...201221883254
efficacy of heat treatment for disinfestation of concrete grain silos.field experiments were conducted in 2007 and 2008 to evaluate heat treatment for disinfestations of empty concrete elevator silos. a mobile heat treatment unit was used to introduce heat into silos to attain target conditions of 50 degrees c for at least 6 h. ventilated plastic containers with a capacity of 100 g of wheat, triticum aestivum l., held rhyzopertha dominica (f.) (coleoptera: bostrichidae) and tribolium castaneum (herbst) (coleoptera: tenebrionidae). polyvinyl chloride containers wit ...201121882711
wheat curl mite resistance: interactions of mite feeding with wheat streak mosaic virus infection.the majority of plant viruses are dependent on arthropod vectors for spread between plants. wheat streak mosaic virus (family potyviridae, genus tritimovirus, wsmv) is transmitted by the wheat curl mite, aceria tosichella keifer, and this virus and vector cause extensive yield losses in most major wheat (triticum aestivum l.)-growing regions of the world. many cultivars in use are susceptible to this vector-virus complex, and yield losses of 10-99% have been documented. wheat curl mite resistanc ...201121882710
changes in phytohormones and fatty acids in wheat and rice seedlings in response to hessian fly (diptera: cecidomyiidae) infestation.phytohormones and fatty acids (fas) play important roles in plant resistance to insects and pathogens. in this study, we investigated the similarities and differences in the accumulations of phytohormones and fas in the resistant wheat (triticum aestivum l.) 'molly' and the nonhost rice (oryza sativa l.) 'niponbare' in responses to hessian fly, mayetiola destructor (say) (diptera: cecidomyiidae), larval attacks. using chemical ionization-gas-chromatography/mass spectrometry, we analyzed the conc ...201121882708
species composition of aphid vectors (hemiptera: aphididae) of barley yellow dwarf virus and cereal yellow dwarf virus in alabama and western florida.yellow dwarf is a major disease problem of wheat, triticum aestivum l., in alabama and is estimated to cause yield loss of 21-42 bu/acre. the disease is caused by a complex of viruses comprising several virus species, including barley yellow dwarf virus-pav and cereal yellow dwarf virus-rpv. several other strains have not yet been classified into a specific species. the viruses are transmitted exclusively by aphids (hemiptera:aphididae). between the 2005 and 2008 winter wheat seasons, aphids wer ...201121882679
combined effect of iron and zinc on micronutrient levels in wheat (triticum aestivum l.).a nutrient solution experiment was conducted to investigate the effect of fe and zn supply on fe, zn, cu, and mn concentrations in wheat plants. the experiment used a factorial combination of two fe levels (0 and 5 mg l(-1)) and three zn levels (0, 0.1 and 10 mg i(-1)). the supply of fe (5 mg l(-1)) and zn (0.1 mg l(-1)) increased plant dry weight and leaf chlorophyll content compared to the fe or zn deficient (0 mg 11) treatments. however, excess zn supply (10 mg l(-1)) reduced plant dry weight ...201121882661
dispersed benzoxazinone gene cluster: molecular characterization and chromosomal localization of glucosyltransferase and glucosidase genes in wheat and rye.benzoxazinones (bxs) are major defensive secondary metabolites in wheat (triticum aestivum), rye (secale cereale), and maize (zea mays). here, we identified full sets of homeologous and paralogous genes encoding bx glucosyltransferase (gt) and bx-glucoside glucosidase (glu) in hexaploid wheat (2n = 6x = 42; aabbdd). four gt loci (tagta-tagtd) were mapped on chromosomes 7a, 7b (two loci), and 7d, whereas four glu1 loci (taglu1a-taglu1d) were on chromosomes 2a, 2b (two loci), and 2d. transcript le ...201121875895
lipid binding interactions of antimicrobial plant seed defence proteins: puroindoline-a and β-purothionin.the indolines and thionins are basic, amphiphilic and cysteine-rich proteins found in cereals; puroindoline-a (pin-a) and β-purothionin (β-pth) are members of these families in wheat (triticum aestivum). pin-a and β-pth have been suggested to play a significant role in seed defence against microbial pathogens, making the interaction of these proteins with model bacterial membranes an area of potential interest. we have examined the binding of these proteins to lipid monolayers composed of 1,2-di ...021869972
intergenic spacer length variants in old portuguese bread wheat cultivars.the intergenic spacer of the ribosomal dna is highly variable, but is location specific in the nucleolar organizer region of the chromosomes. this study provides an event of high level of polymorphism / size variation and occurrence of 14 unique phenotypes in 48 landraces of portuguese bread wheat cultivars for igs-amplified products obtained by pcr-rflp technique performed with taqi. the attendant igs polymorphism has been used to deduce affinities between landraces. some of the high molecular ...201121869468
[morphological and genetic characteristics of leaf hairiness in wheat (triticum aestivum l.) as analyzed by computer-aided phenotyping].computer-aided image processing was used to study the morphology of leaf hairiness in the wheat cultivars saratovskaya 29 and golubka, as well as the introgressed strain 102/00i of the cultivar rodina carrying the hairiness control gene introgressed from aegilops speltoides. morphological differences in leaf hairiness were detected and described in detail. the genetic control ofhairiness was studied in two cultivars (golubka and saratovskaya 29) with similar hairiness patterns. crossing these cu ...201121866865
genetic transformation of mature embryos of bread (t. aestivum) and pasta (t. durum) wheat genotypes.the objective of the present study is to develop an efficient protocol for regeneration of transgenic wheat plants using agrobacterium- mediated transformation of mature embryos of hexaploid bread wheat (triticum aestivum) and tetraploid pasta wheat (triticum durum). the data indicated that embryogenic calli were formed within 7 days in the presence of 2 mgl-1 2,4-d. adventitious shoots emerged from the embryonic calli in the presence of 2 mgl-1 ba. shoot regeneration frequency varied between wh ...201021865876
PPIase activities and interaction partners of FK506-binding proteins in the wheat thylakoid.FK506-binding proteins (FKBPs) and cyclophilins, collectively called immunophilins, conserve peptidyl-prolyl cis/trans isomerase (PPIase) active sites, although many lack PPIase activity. The chloroplast thylakoid contains a large proportion of the plant immunophilin family, but their functions within this compartment are unclear. Some lumenal immunophilins are important for assembly of photosynthetic complexes, implicating them in the maintenance and turnover of the photosynthetic apparatus dur ...201121848652
transformation of the us bread wheat 'butte 86' and silencing of omega-5 gliadin genes.complex groups of proteins determine the unique functional properties of wheat flour and are sometimes responsible for food intolerances and allergies in individuals that consume wheat products. transgenic approaches can be used to explore the functions of different flour proteins, but are limited to the few wheat cultivars that can be transformed and also by the lack of detailed information about genes and proteins expressed in grain from those cultivars. the us bread wheat butte 86 has been ex ...201221844700
molecular, phylogenetic and comparative genomic analysis of the cytokinin oxidase/dehydrogenase gene family in the poaceae.the genomes of cereals such as wheat (triticum aestivum) and barley (hordeum vulgare) are large and therefore problematic for the map-based cloning of agronomicaly important traits. however, comparative approaches within the poaceae permit transfer of molecular knowledge between species, despite their divergence from a common ancestor sixty million years ago. the finding that null variants of the rice gene cytokinin oxidase/dehydrogenase 2 (osckx2) result in large yield increases provides an opp ...021838715
pcr-based isolation and identification of full-length low-molecular-weight glutenin subunit genes in bread wheat (triticum aestivum l.).low-molecular-weight glutenin subunits (lmw-gss) are encoded by a multi-gene family and are essential for determining the quality of wheat flour products, such as bread and noodles. however, the exact role or contribution of individual lmw-gs genes to wheat quality remains unclear. this is, at least in part, due to the difficulty in characterizing complete sequences of all lmw-gs gene family members in bread wheat. to identify full-length lmw-gs genes, a polymerase chain reaction (pcr)-based met ...201121830110
intake and digestion of whole-crop barley and wheat silages by dairy heifers.the effect of maturity at harvest on the digestibility and intake of large bale silage made from whole-crop barley and wheat when fed to growing heifers was evaluated. two crops of spring barley (hordeum distichum cv. filippa and kinnan) and 1 of winter wheat (triticum aestivum cv. olevin) were harvested at the heading, milk, and dough stages of maturity. the silage was fed to 36 dairy heifers in a balanced crossover experiment with 3 periods and 9 treatments (diets based on 3 crops and 3 stages ...201121821807
two wheat (triticum aestivum) pathogenesis-related 10 (pr-10) transcripts with distinct patterns of abundance in different organs.pr-10 genes encode small, acidic, intracellular proteins that respond to abiotic and biotic stimuli. transgenic expression of pr-10 genes has been shown to enhance early seedling growth of dicots in saline environments. to identify candidate pr-10 genes in cereals for increasing stress tolerance, we conducted phylogenetic analyses and real-time polymerase chain reaction of representatives of the two major clades of putative pr-10 genes in wheat. we observed that the abundance of bq752893 was gen ...021818707
differential effects of a post-anthesis fertilizer regimen on the wheat flour proteome determined by quantitative 2-de.mineral nutrition during wheat grain development has large effects on wheat flour protein content and composition, which in turn affect flour quality and immunogenic potential for a commodity of great economic value. however, it has been difficult to define the precise effects of mineral nutrition on protein composition because of the complexity of the wheat flour proteome. recent improvements in the identification of flour proteins by tandem mass spectrometry (ms/ms) and the availability of a c ...201121816081
redox changes during cold acclimation affect freezing tolerance but not the vegetative/reproductive transition of the shoot apex in wheat.cold acclimation is necessary for winter wheat (triticum aestivum l.) to achieve its genetically determined maximum freezing tolerance, and cold also fulfils the vernalisation requirement. chromosome 5a is a major regulator of these traits. the aim of the present study was to discover whether changes in the half-cell redox potential of the glutathione/glutathione disulphide (gsh/gssg) and ascorbate/dehydroascorbate (aa/dha) couples induced by cold acclimation are related to freezing tolerance an ...201121815980
comparative proteome analysis of glutenin synthesis and accumulation in developing grains between superior and poor quality bread wheat cultivars.wheat glutenins are the major determinants of wheat quality. in this study, grains at the development stage from three wheat cultivars (jimai 20, jin 411 and zhoumai 16) with different bread-making quality were harvested based on thermal times from 150 °c(d) to 750 °c(d) , and were used to investigate glutenin accumulation patterns and their relationships with wheat quality.201221815156
identification of changes in wheat (triticum aestivum l.) seeds proteome in response to anti-trx s gene.thioredoxin h (trx h) is closely related to germination of cereal seeds. the cdna sequences of the thioredoxin s (trx s) gene from phalaris coerulescens and the thioredoxin h (trx h) gene from wheat are highly homologous, and their expression products have similar biological functions. transgenic wheat had been formed after the antisense trx s was transferred into wheat, and it had been certified that the expression of trx h decreased in transgenic wheat, and transgenic wheat has high resistance ...201121811579
a major invasion of transposable elements accounts for the large size of the blumeria graminis f.sp. tritici genome.powdery mildew of wheat (triticum aestivum l.) is caused by the ascomycete fungus blumeria graminis f.sp. tritici. genomic approaches open new ways to study the biology of this obligate biotrophic pathogen. we started the analysis of the bg tritici genome with the low-pass sequencing of its genome using the 454 technology and the construction of the first genomic bacterial artificial chromosome (bac) library for this fungus. high-coverage contigs were assembled with the 454 reads. they allowed t ...201121809124
bioremediation and detoxification of synthetic wastewater containing triarylmethane dyes by aeromonas hydrophila isolated from industrial effluent.economical and bio-friendly approaches are needed to remediate dye-contaminated wastewater from various industries. in this study, a novel bacterial strain capable of decolorizing triarylmethane dyes was isolated from a textile wastewater treatment plant in greece. the bacterial isolate was identified as aeromonas hydrophila and was shown to decolorize three triarylmethane dyes tested within 24?h with color removal in the range of 72% to 96%. decolorization efficiency of the bacterium was a func ...201121808740
fusarium graminearum forms mycotoxin producing infection structures on wheat.abstract: background: the mycotoxin producing fungal pathogen fusarium graminearum is the causal agent of fusarium head blight (fhb) of small grain cereals in fields worldwide. although f. graminearum is highly investigated by means of molecular genetics, detailed studies about hyphal development during initial infection stages are rare. in addition, the role of mycotoxins during initial infection stages of fhb is still unknown. therefore, we investigated the infection strategy of the fungus on ...201121798058
chromosomal and genome-wide molecular changes associated with initial stages of allohexaploidization in wheat can be transit and incidental.genomic instability can be induced by nascent allopolyploidization in plants. however, most previous studies have not defined to what extent the allopolyploidy-induced rapid genomic instability represents a general response, and hence important to evolution, or merely incidental events occurring stochastically in a limited number of individuals. we report here that in a newly formed allohexaploid wheat line between tetraploid wheat triticum turgidum subsp. durum (genome bbaa) and aegilops tausch ...201121797821
factors responsible for deep-sowing tolerance in wheat seedlings: varietal differences in cell proliferation and the co-ordinated synchronization of epidermal cell expansion and cortical cell division for the gibberellin-mediated elongation of first internodes.a wheat cultivar, triticum aestivum 'hong mang mai', shows tolerance to deep-sowing conditions by extreme elongation of the first internode, likely mediated by the gibberellin (ga) response. to understand factors involved in the response of this deep-sowing-tolerant cultivar, cell expansion and division that confer elongation on the first internodes of wheat seedlings were investigated.201121791455
[the role of chromosomes from homeologous group 5 in regulation of grain hardness and protein content in substitution lines of common wheat cultivar saratovskaya 29].genetic regulation of grain hardness and protein content in intervarietal substitution lines for chromosomes of homeologous group 5 was examined. common wheat cultivar saratovskaya 29 with high bread-backing properties served as the recipient. donors of chromosomes 5a and 5d were 18 cultivars with variable traits examined, including high-protein cultivars (atlas 66 and diamant 2), and soft-grain cultivars (ul'yanovka and chinese spring). analysis of substitution lines pointed to a substantial ef ...201121786668
[ecotoxicity of synthetical musks on wheat (triticum aestivum) based on seed germination].with soil culture the ecotoxicological effects of galaxolide (hhcb) and tonalide (ahtn) on the germination of wheat (triticum aestivum) seeds were investigated. and the influence of these two synthetical musks on the wheat germination rate, inhibition rate of root elongation and inhibition rate of shoot elongation were studied. the results showed that there were no obvious dose-effect relationship between wheat germination rate and concentrations of these two synthetical musks. lower dose (hhcb ...201121780608
use of methylation filtration and c(0)t fractionation for analysis of genome composition and comparative genomics in bread wheat.we investigated the compositional and structural differences in sequences derived from different fractions of wheat genomic dna obtained using methylation filtration and c(0)t fractionation. comparative analysis of these sequences revealed large compositional and structural variations in terms of gc content, different structural elements including repeat sequences (e.g., transposable elements and simple sequence repeats), protein coding genes, and non-coding rna genes. a correlation between meth ...201121777856
the spectrum of low molecular weight alpha-amylase/protease inhibitor genes expressed in the us bread wheat cultivar butte 86.abstract: background: wheat grains accumulate a variety of low molecular weight proteins that are inhibitors of alpha-amylases and proteases and play an important protective role in the grain. these proteins have more balanced amino acid compositions than the major wheat gluten proteins and contribute important reserves for both seedling growth and human nutrition. the alpha-amylase/protease inhibitors also are of interest because they cause ige-mediated occupational and food allergies and there ...201121774824
biodegradation of 2-mercaptobenzothiazolyl-(z)-(2-aminothiazol-4-yl)-2-(tert-butoxycarbonyl) isopropoxyiminoacetate by pseudomonas desmolyticum ncim 2112.2-mercaptobenzothiazolyl-(z)-(2-aminothiazol-4-yl)-2-(tert-butoxycarbonyl) isopropoxyiminoacetate is used as supplementary additives in commercial-grade insecticides to compensate for the time factor needed for the actual pesticide chemical to start its action. this investigation describes the biodegradation of 2-mercaptobenzothiazolyl-(z)-(2-aminothiazol-4-yl)-2-(tert-butoxycarbonyl) isopropoxyiminoacetate by pseudomonas desmolyticum ncim 2112. the biodegradation is influenced by other carbon a ...201121773762
identification of a myb3r gene involved in drought, salt and cold stress in wheat (triticum aestivum l.).abiotic stress seriously affects crop growth and productivity. to better understand the mechanisms plant uses to cope with drought, cold and salt stress, it is necessary to isolate and characterize important regulators response to these stresses. in this study, we cloned a myb gene from wheat (triticum aestivum l.) and designated it as tamyb3r1 based on its conserved three repeats in myb domain. the sequence of tamyb3r1 protein shares high identity to other plant myb3r proteins. subcellular loca ...201121763408
genetic analysis of carbon isotope discrimination and its relation to yield in a wheat doubled haploid population.carbon isotope discrimination (δ(13)c) is considered a useful indicator for indirect selection of grain yield (gy) in cereals. therefore, it is important to evaluate the genetic variation in δ(13)c and its relationship with gy. a doubled haploid (dh) population derived from a cross of two common wheat varieties, hanxuan 10 (h10) and lumai 14 (l14), was phenotyped for δ(13)c in the flag leaf, gy and yield associated traits in two trials contrasted by water availability, specifically, rain-fed and ...021762383
deciphering the genetics of flowering time by an association study on candidate genes in bread wheat (triticum aestivum l.).earliness is very important for the adaptation of wheat to environmental conditions and the achievement of high grain yield. a detailed knowledge of key genetic components of the life cycle would enable an easier control by the breeders. the objective of the study was to investigate the effect of candidate genes on flowering time. using a collection of hexaploid wheat composed of 235 lines from diverse geographical origins, we conducted an association study for six candidate genes for flowering ...201121761163
identification and mapping of a new powdery mildew resistance gene on chromosome 6d of common wheat.powdery mildew, caused by blumeria graminis f. sp. tritici, is one of the most serious wheat diseases. the rapid evolution of the pathogen's virulence, due to the heavy use of resistance genes, necessitates the expansion of resistance gene diversity. the common wheat line d57 is highly resistant to powdery mildew. a genetic analysis using an f(2) population derived from the cross of d57 with the susceptible cultivar yangmai 158 and the derived f(2:3) lines indicated that d57 carries two dominant ...201121755339
efficacy and tolerability of fitostimoline in two different forms (soaked gauzes and cream) and citrizan gel in the topical treatment of second-degree superficial cutaneous burns.a total of 227 patients (mean age 41.3 years, 52% females) with at least one second-degree superficial cutaneous burn of thermal origin of a smallest transverse diameter =20?mm and a largest transverse diameter =90?mm were randomised to receive the topical application of aqueous extract of triticum vulgare (fitostimoline) in two different forms (soaked gauzes and cream) or catalase of horse origin in form of gel (citrizan gel), given up to healing or to a maximum of 20 days. the rate of lesion h ...201121747844
pattern of solutes accumulated during leaf osmotic adjustment as related to duration of water deficit for wheat at the reproductive stage.this study examined expression of osmotic adjustment (oa) and accumulation of solutes in wheat (triticum aestivum l.) leaves in response to water deficit (wd) imposed at the reproductive stage. two contrasting cultivars, hartog and sunco (putatively high and low in oa capacity, respectively), were grown in deep (viz. 80 cm) pots in a controlled environment. in a sandy substrate, leaf oa was 5-times greater in hartog compared with sunco. at 21 d of wd treatment, k(+) only accounted for 12% of oa ...201121741263
precise mapping fhb5, a major qtl conditioning resistance to fusarium infection in bread wheat (triticum aestivum l.).qfhi.nau-5a is a major quantitative trait locus (qtl) against fusarium graminearum infection in the resistant wheat germplasm wangshuibai. genetic analysis using bc(3)f(2) and bc(4)f(2) populations, derived from selfing two near-isogenic lines (nil) heterozygous at qfhi.nau-5a that were developed, respectively, with mianyang 99-323 and ph691 as the recurrent parent, showed that qfhi.nau-5a inherited like a single dominant gene. this qtl was thus designated as fhb5. to fine map it, these two back ...201121739138
selection of phytotoxin producing rhizobacteria.in order to select phytotoxin producing rhizobacteria to control weed plants, twenty five bacterial strains previously isolated from the rhizospheres of various plants were grown in a liquid medium and, after cell removal by centrifugation, the liquid phases were freeze-dried and the products were extracted with ethyl acetate/methanol. the extracts were concentrated to dryness under vacuum and dissolved in water and sucrose solution to be submitted to in vitro assays of lettuce (lactuca sativa l ...201121739082
modeling and phylogeny analysis of bread wheat mnsod.superoxide dismutase (sod) acts as first line of defense against oxidative and genetic stress. manganese superoxide dismutase (mnsod), found in mitochondria or peroxisomes, contains mn(iii) at the active site. therefore, it is of interest to study mnsod from bread wheat (a grain crop). however, a structure model is not yet solved for bread wheat mnsod. hence, we describe the structure model of bread wheat mnsod developed using homology model. the model provides molecular insight to metal binding ...201121738317
inhibition by wheat sprout (triticum aestivum) juice of bisphenol a-induced oxidative stress in young women.for health of future generation, fertile young women should be monitored for exposure of endocrine disrupting chemicals (edcs). among edcs, bisphenol a (bpa) is suggested to induce reactive oxygen species (ros) which play an important role in pathologies of female diseases such as endometriosis. on the other hand, previous studies suggested that sprouts of wheat (triticum aestivum) have antimutagenicity and antioxidant activity. we performed the 2 weeks intervention of wheat sprout juice (100ml/ ...201121736952
discovery of english grain aphid (hemiptera: aphididae) biotypes in china.the english grain aphid, sitobion avenae (f.) (hemiptera: aphididae), is an important pest insect of wheat, triticum aestivum (l.), in china. grain aphid biotypes are necessary to breed aphid-resistant wheat varieties; however, none have currently been identified. here, we describe a method to identify grain aphid biotypes and survey the aphid biotype variation in the wheat growth area of china. clones of s. avenae were collected from 11 locations in china and used to establish culture populatio ...201121735932
approved quarantine treatment for hessian fly (diptera: cecidomyiidae) in large-size hay bales and hessian fly and cereal leaf beetle (coleoptera: chrysomelidae) control by bale compression.a quarantine treatment using bale compression (32 kg/cm2 pressure) and phosphine fumigation (61 g/28.3 m3 aluminum phosphide for 7 d at 20 degrees c) was approved to control hessian fly, mayetiola destructor (say), in large-size, polypropylene fabric-wrapped bales exported from the western states to japan. no hessian fly puparia (45,366) survived to the adult stage in infested wheat, triticum aestivum l., seedlings exposed to the treatment in a large-scale commercial test. daily temperatures (me ...201121735895
decolorization of different dyes by a newly isolated white-rot fungi strain ganoderma sp.en3 and cloning and functional analysis of its laccase gene.a laccase-producing white-rot fungi strain ganoderma sp.en3 was newly isolated from the forest of tzu-chin mountain in china. ganoderma sp.en3 had a strong ability of decolorizing four synthetic dyes, two simulated dye bath effluents and the real textile dye effluent. induction in the activity of laccase during the decolorization process indicated that laccase played an important role in the efficient decolorization of different dyes by this fungus. phytotoxicity study with respect to triticum a ...201121733624
effects of sterol-binding agent nystatin on wheat roots: the changes in membrane permeability, sterols and glycoceramides.plant sterols are important multifunctional lipids, which are involved in determining membrane properties. biophysical characteristics of model lipid and isolated animal membranes with altered sterol component have been intensively studied. in plants however, the precise mechanisms of involvement of sterols in membrane functioning remain unclear. in present work the possible interactions between sterols and other membrane lipids in plant cells were studied. a useful experimental approach for elu ...201121726881
heterotrimeric gα subunit from wheat (triticum aestivum), ga3, interacts with the calcium-binding protein, clo3, and the phosphoinositide-specific phospholipase c, pi-plc1.the canonical gα subunit of the heterotrimeric g protein complex from wheat (triticum aestivum), ga3, and the calcium-binding protein, clo3, were revealed to interact both in vivo and in vitro and clo3 was shown to enhance the gtpase activity of ga3. clo3 is a member of the caleosin gene family in wheat with a single ef-hand domain and is induced during cold acclimation. bimolecular fluorescent complementation (bifc) was used to localize the interaction between clo3 and ga3 to the plasma membran ...201121725861
field controlled experiments of mercury accumulation in crops from air and soil.field open top chambers (otcs) and soil mercury (hg) enriched experiments were employed to study the influence of hg concentrations in air and soil on the hg accumulation in the organs of maize (zea mays l.) and wheat (triticum aestivum l.). results showed that hg concentrations in foliages were correlated significantly (p < 0.05) with air hg concentrations but insignificantly correlated with soil hg concentrations, indicating that hg in crop foliages was mainly from air. hg concentrations in ro ...201121723013
identification of differentially expressed genes in three-pistil mutation in wheat using annealing control primer system.the common wheat line three-pistil (tp) is a valuable mutant for wheat breeding. the tp mutation has normal spike morphology; however, it only produces three pistils per floret. therefore, it has potential to increase the grain number per spike. in order to determine the underlying molecular mechanism, an annealing control primer system was used to identify the different expressed genes in three-pistil mutation. using 120 arbitrary acp primers, we identified three differentially expressed genes ...201121722717
assessment of genetic diversity of wheat genotypes by resistance gene analog-est markers.resistance gene analog-expressed sequence tag (rga-est)-based markers have been used for variety discrimination and studies of genetic diversity in wheat. our aim is to increase the competitiveness of public wheat breeding programs through intensive use of modern selection technologies, mainly marker-assisted selection. the genetic diversity of 77 wheat nucleotide binding site (nbs)-containing rga-ests was assessed. resistant and susceptible bread wheat (triticum aestivum) genotypes were used as ...201121710462
distribution of gluten proteins in bread wheat (triticum aestivum) grain.gluten proteins are the major storage protein fraction in the mature wheat grain. they are restricted to the starchy endosperm, which forms white flour on milling, and interact during grain development to form large polymers which form a continuous proteinaceous network when flour is mixed with water to give dough. this network confers viscosity and elasticity to the dough, enabling the production of leavened products. the starchy endosperm is not a homogeneous tissue and quantitative and qualit ...201121693664
association mapping of quantitative resistance to phaeosphaeria nodorum in spring wheat landraces from the usda national small grains collection.stagonospora nodorum blotch (snb), caused by phaeosphaeria nodorum, is a destructive disease of wheat (triticum aestivum l.) found throughout the united states. host resistance is the only economically feasible option for managing the disease; however, few snb resistant wheat cultivars are known to exist. in this study, we report findings from an association mapping (am) of resistance to p. nodorum in 567 spring wheat landraces of diverse geographic origin. the accessions were evaluated for seed ...201121692647
genetic differentiation at microsatellite loci among populations of mycosphaerella graminicola from california, indiana, kansas and north dakota.mycosphaerella graminicola causes septoria tritici blotch (stb) in wheat (triticum aestivum l.) and is considered one of the most devastating pathogens of that crop in the united states. although the genetic structures of m. graminicola populations from different countries have been analyzed using various molecular markers, relatively little is known about m. graminicola populations from geographically distinct areas of the united states and, in particular, of those from spring versus winter whe ...201121692645
nema, a functional-structural model of nitrogen economy within wheat culms after flowering. i. model description.models simulating nitrogen use by plants are potentially efficient tools to optimize the use of fertilizers in agriculture. most crop models assume that a target nitrogen concentration can be defined for plant tissues and formalize a demand for nitrogen, depending on the difference between the target and actual nitrogen concentrations. however, the teleonomic nature of the approach has been criticized. this paper proposes a mechanistic model of nitrogen economy, nema (nitrogen economy model with ...201121685431
nema, a functional-structural model of nitrogen economy within wheat culms after flowering. ii. evaluation and sensitivity analysis.simulating nitrogen economy in crop plants requires formalizing the interactions between soil nitrogen availability, root nitrogen acquisition, distribution between vegetative organs and remobilization towards grains. this study evaluates and analyses the functional-structural and mechanistic model of nitrogen economy, nema (nitrogen economy model within plant architecture), developed for winter wheat (triticum aestivum) after flowering.201121685429
profiles of phenolic compounds in modern and old common wheat varieties determined by liquid chromatography coupled with time-of-flight mass spectrometry.the health-promoting properties of common wheat (triticum aestivum l.) have been largely attributed to the presence of unique phytochemicals of whole grains. the aim of this study was to profile the phenolic content of 16 old and 6 modern italian wheat varieties, cropped in the same location and growing season. high variability was observed among the investigated wheat genotypes, both in the free and bound phenolic extracts. the total polyphenol content ranged from 885.5 to 1715.9 μmol gae/100 g ...201121683368
molecular cloning and variation of ω-gliadin genes from a somatic hybrid introgression line ii-12 and parents (triticum aestivum cv. jinan 177 and agropyron elongatum). 201121677400
physical localization of nors and its length variants in old portuguese durum wheat cultivars.the variation at the internal transcribed spacer (its) region of the ribosomal dna has been correlated with the number of nucleolar organizer regions (nors) in some plant species. besides, the number of nors might influence the rate of homogenization of the rdna repeats. in recent studies, its length variants were detected in bread wheat cultivars but no reports about their presence in durum wheat were found. in the present study, we localized and identified the nors of 51 old portuguese durum w ...201121677393
[development of commercially valuable traits in hexaploid triticale lines with aegilops introgressions as dependent on the genome composition].introgressive hybridization is an efficient means to improve the genetic diversity of cultivated cereals, including triticale. to identify the triticale lines with aegilops introgressions, genotyping was carried out with ten lines obtained by crossing hexaploid triticale with genome-substitution forms of the common wheat cultivar avrora: avrolata (aabbuu), avrodes (aabbss), and avrotika (aabbtt). the genome composition of the triticale lines was studied by in situ hybridization, and recombinatio ...021675241
molecular and physiological strategies to increase aluminum resistance in plants.aluminum (al) toxicity is a primary limitation to plant growth on acid soils. root meristems are the first site for toxic al accumulation, and therefore inhibition of root elongation is the most evident physiological manifestation of al toxicity. plants may resist al toxicity by avoidance (al exclusion) and/or tolerance mechanisms (detoxification of al inside the cells). the al exclusion involves the exudation of organic acid anions from the root apices, whereas tolerance mechanisms comprise int ...201121660471
[effects of nitrogen application and elevated atmospheric co2 on electron transport and energy partitioning in flag leaf photosynthesis of wheat].wheat (triticum aestivum) plants were pot-cultured in open top chambers at the nitrogen application rate of 0 and 200 mg x kg(-1) soil and the atmospheric co2 concentration of 400 and 760 micromol x mol(-1). through the determination of flag leaf nitrogen and chlorophyll contents, photosynthetic rate (pn)-intercellar co2 concentration (ci) response curve, and chlorophyll fluorescence parameters at heading stage, the photosynthetic electron transport rate and others were calculated, aimed to inve ...201121657023
genetic analysis of resistance to septoria tritici blotch in the french winter wheat cultivars balance and apache.the ascomycete mycosphaerella graminicola is the causal agent of septoria tritici blotch (stb), one of the most destructive foliar diseases of bread and durum wheat globally, particularly in temperate humid areas. a screening of the french bread wheat cultivars apache and balance with 30 m. graminicola isolates revealed a pattern of resistant responses that suggested the presence of new genes for stb resistance. quantitative trait loci (qtl) analysis of a doubled haploid (dh) population with fiv ...201121655994
genome-wide gene expression analysis supports a developmental model of low temperature tolerance gene regulation in wheat (triticum aestivum l.).to identify the genes involved in the development of low temperature (lt) tolerance in hexaploid wheat, we examined the global changes in expression in response to cold of the 55,052 potentially unique genes represented in the affymetrix wheat genome microarray. we compared the expression of genes in winter-habit (winter norstar and winter manitou) and spring-habit (spring manitou and spring norstar)) cultivars, wherein the locus for the vernalization gene vrn-a1 was swapped between the parental ...021649926
association mapping of dynamic developmental plant height in common wheat.drought as a major abiotic stress often occurs from stem elongation to the grain filling stage of wheat in northern china. plant height (ph) is a suitable trait to model the dissection of drought tolerance. the purposes of the present study were to validate molecular markers for ph developmental behavior and identify elite alleles of molecular markers. after the phenotyping of 154 accessions for ph dynamic development under well-watered (ww) and drought stressed (ds) conditions, and the genotypi ...201121647605
autoimmune response and repression of mitotic cell division occur in inter-specific crosses between tetraploid wheat and aegilops tauschii coss. that show low temperature-induced hybrid necrosis.common wheat is an allohexaploid species originating from a naturally occurring inter-specific cross between tetraploid wheat and the diploid wild wheat aegilops tauschii coss. artificial allopolyploidization can produce synthetic hexaploid wheat. however, synthetic triploid hybrids show four types of hybrid growth abnormalities: type ii and iii hybrid necrosis, hybrid chlorosis, and severe growth abortion. of these hybrid abnormalities, type ii necrosis is induced by low temperature. under low ...201121645146
the effect of extremely diluted agitated gibberellic acid (10e-30) on wheat stalk growth--a two researcher pilot study.use of a wheat growth bio assay after 7 days in research on homeopathic dilutions of gibberellic acid.201121641523
automated flow-based anion-exchange method for high-throughput isolation and real-time monitoring of rubisco in plant extracts.in this work, a miniaturized, completely enclosed multisyringe-flow system is proposed for high-throughput purification of rubisco from triticum aestivum extracts. the automated method capitalizes on the uptake of the target protein at 4°c onto q-sepharose fast flow strong anion-exchanger packed in a cylindrical microcolumn (105 × 4 mm) followed by a stepwise ionic-strength gradient elution (0-0.8 mol/l nacl) to eliminate concomitant extract components and retrieve highly purified rubisco. the m ...201121641435
dynamic management of crop diversity: from an experimental approach to on-farm conservation.in agricultural systems, biodiversity includes diversity within species and among species and provides many benefits for production, resilience and conservation. this article addresses the effects of a strategy of in situ conservation called dynamic management (dm) on population evolution, adaptation and diversity. two french dm initiatives are considered, the first one corresponding to an experimental context, the second to an on-farm management. results from a study over 26 years of experiment ...201121640955
genetic diversity analysis of abiotic stress response gene tasnrk2.7-a in common wheat.sucrose non-fermenting1-related protein kinase 2 (snrk2) plays a key role in plant stress signaling transduction pathways. in this study, one copy of tasnrk2.7, a snrk2 member of common wheat, was isolated and characterized for nucleotide diversity among 45 wheat accessions with different stress-response features. most of the accessions were elite wheat cultivars, which had been subject to population bottlenecks and intensive selection during breeding. nucleotide and haplotype diversity across t ...201121637995
Displaying items 3601 - 3700 of 7762