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[serological analysis of two complex salmonella respectively arizona o-groups (salmonella o:48 and o:64 - arizona o:5 and o:29) with the object of combining them into one salmonella o-group 48 within the kauffmann-white-schema (author's transl)].in 1963 kauffmann divided o-group y (= o:48) of the kauffmann-white-schema into 3 sub-groups, e.g. 481, 482 - s. dahlem; 481, 482, 483 - s. djakarta; 481, 483, 484 = citrobacter no. 2624/36. he also recommended the use of the two serotypes s. dahlem and s. djakarta for the preparation of a diagnostic group-serum. at that time, serological relations- especially o-antigenic relations (and even some identities) - between the separate salmonella and arizona genera were known, viz. between the salmon ...197665071
[live enteric typhoid vaccine consisting of a vi-negative double-dependent mutant of s. typhi and a vi-positive strain of citrobacter].the authors present experimental data on the study of the living enteral vaccine against the typhoid infection from the vi-negative strain of salmonella with a double-dependence by streptomycin and purine, and from the vi-positive strain -- citrobacter 5396/38. the method of immunoelectrophoretic analysis showed an indenticity of the o- and h-antigens of the doubledependent mutant with the o- and h-antigens of the typhoid strains of bacteria (ty2-4446 and 5501). a sufficiently marked immunologic ...197660853
immunological relationships of bacterial l-asparaginases.rabbit antisera against l-asparaginase preparations from escherichia coli, erwinia carotovora, citrobacter sp. and chromobacterium violaceum showed on immunoelectrophoresis that only the enzymes from e. coli and citrobacter are immunologically related. purified preparations had to be used to determine the immunological cross-reactions. immunoelectrophoresis at different ph values yielded the zero mobility points of the enzymes. the activity of the er. carotovora preparation was enhanced up to fo ...197654404
importance of colicinogeny for the course of acute bacillary dysentery.the development of acute bacillary dysentery was followed in 23 patients involved in two outbreaks and in three sporadic, mutually unrelated cases. repeated cultivations performed at 2-day intervals for 10 days yielded 386 identifiable strains of "opportune intestinal flora". escherichia coli colicinogenic activity is one of the significant factors of gastrointestinal tract protection. the period of shigella excretion is significantly reduced (p less than 0.02) if an appropriate colicinogenic e. ...197944941
resistance of gram-negative bacteria to antibiotics in large calf agglomerations.the antibiotic resistance of e. coli, citrobacter, enterobacter-klebsiella and pseudomonas aeruginosa strains isolated from calves was tested. a high proportion of multiresistance was found even in e. coli strains isolated from newborn calves. gram-negative bacteria isolated from animals in three large calfhouses were almost 100% resistant to ampicillin, tetracyclines and sulphonamides. multiresistance was general and varied from 5 to 12 antibiotics among different strains. initial high sensitiv ...197941425
[metabolism of dicarboxylic amino acids and their amides in bacteria of the genus citrobacter].in 58 citrobacter strains the pathways of the utilization of dicarbonic amino acids and their amides were studied. these organisms were found to be incapable of decarboxylating glutaminic and asparaginic acids, as well as their amides. all the strains could actively desamidizate asparagine. not all of these strains showed glutaminase activity. aspartate-aminotransferase occurred twice as often as alanine-aminotransferase, the level of activity being approximately the same. the citrobacter strain ...197937683
studies of relationship among terrestrial pseudomonas, alcaligenes, and enterobacteria by an immunological comparison of glutamine synthetase.antibody to purified glutamine synthetase from escherichia coli was prepared and used for an immunological comparison of glutamine synthetases from species of salmonella, citrobacter, enterobacter, serratia, proteus, erwinia, aeromonas, pseudomonas, acinetobacter, xanthomonas, alcaligenes, and paracoccus. the results of ouchterlony double diffusion experiments and quantitative microcomplement fixation studies indicated that the amino acid sequence of this enzyme was highly conserved in different ...197831146
significance of microbial contamination of stored cadaver kidneys.the importance of microbial contamination of cadaver kidneys was assessed in 83 consecutively stored and transplanted kidneys. fourteen kidneys had a single positive culture during storage and five had multiple positive cultures. only one postoperative infection could be traced to kidney contamination during storage (candida wound infection). in three of 64 patients who received noncontaminated kidneys, posttransplant wound infections developed. no wound infections occurred in 35 patients who re ...197825059
effect of methionine sulfone on the growth of citrobacter intermedius c3 (author's transl).specific activity of glutamate dehydrogenase (gd) and glutamate synthase (gts) has been determined in the wild strain c3 and on a non excreting pro- mutant strain. methionine sulfone shows inhibitory effects on their growth. the addition of alpha-ketoglutarate to the medium prevents the inhibitory effect and increases the gts specific activity in both strains. the physiological effect of methionine sulfone and its suppression by alpha-ketoglutarate is discussed.197719817
two distinct types of trimethoprim-resistant dihydrofolate reductase specified by r-plasmids of different compatibility groups.r-plasmids from a number of trimethoprim-resistant escherichia coli and citrobacter sp. were studied after transfer to e. coli k12 hosts. each was found to specify a dihydrofolate reductase which was resistant to trimethoprim and methotrexate, and which could be completely separated from the host chromosomal enzyme by gel filtration. two distinct types of r-plasmid dihydrofolate reductases were identified. type i enzymes, typified by the r483 enzyme previously described (sköld, o., and widh, a. ...197714961
approach to a practical method for screening and identifying microorganism genera from urine (author's transl).in this study the author reported upon a practical new system for screening and identifying the microbial agents causing urinary tract infections. this system is composed of a combination of 3 screening procedures (ph-value + nitrite-test + catalase-test) and 8 selective culture media for the purpose of genus identification within 24 hours (uripret-g). a total of 130 cultures was investigated. the employed microorganisms were mainly recovered from urine samples. they included the following speci ...197611179
in vitro study of netilmicin compared with other aminoglycosides.netilmicin (sch 20569) is an ethyl derivative of gentamicin c(1a) that is active against most enterobacteriaceae, pseudomonas aeruginosa, and staphylococcus aureus isolates. among 342 clinical isolates tested, all staphylococci; 92% of escherichia coli, 93% of klebsiella pneumoniae, and 92% of enterobacter were inhibited by 0.8 mug or less of netilmicin per ml, but only 78% of p. aeruginosa were inhibited by 3.1 mug or less per ml. most clinical isolates of enterococci, serratia marcescens, and ...197610829
observations on brilliant green agar with h2s indicator.several formulations of brilliant green agar with an added h2s indicator were evaluated. results were optimum with variations of a basic formula consisting of 40 g of tryptic soy agar (difco), 8 g of lactose, 8 g of sucrose, 80 mg of phenol red, 1 g of sulfanilamide, 1.5 g of ferric ammonium citrate, 5 g of sodium thiosulfate pentahydrate, and 7 mg of brilliant green dye per liter. brilliant green dye was added after sterilization of the other components this formulation supported good growth of ...19767190
kinetic properties of serratia marcescens adenosine 5'-diphosphate glucose pyrophosphorylase.the regulatory properties of partially purified adenosine 5'-diphosphate-(adp) glucose pyrophosphorylase from two serratia marcescens strains (atcc 274 and atcc 15365) have been studied. slight or negligible activation by fructose-p2, pyridoxal-phosphate, or reduced nicotinamide adenine dinucleotide phosphate (nadph) was observed. these compounds were previously shown to be potent activators of the adpglucose pyrophosphorylases from the enterics, salmonella typhimurium, enterobacter aerogenes, e ...19766432
gentamicin-susceptibility of various pathogens isolated from clinical materials.we studied on the antibacterial activity of gentamicin against various pathogens isolated from clinical materials mainly isolated during 1974 and 1975, comparing with other antibiotics. beta hemolytic streptococci, pneumococci and enterococci are less susceptible to gentamicin than staphylococci. staph, aureus and staph. epidermidis resistant to various antibiotics are very susceptible to gentamicin, and no resistant strain to this drug was found. haemophilus influenzae, h. parainfluenzae and h. ...19765619
disruption of vi bacteriophage iii and localization of its deacetylase activity.it has been shown that particles of vi bacteriophage iii catalyse deacetylation of o-acetyl pectic (polygalacturonic) acid, a structural analogue of vi polysaccharide (vi antigen). using this substrate, and determining the acetic acid liberated by gas-liquid chromatogrphy, a method for the estimation of vi phage deacetylase activity has been developed. purified particles of vi phage iii were exposed to a variety of mildly dissociative reagents and conditions, and then tested for plaque-forming a ...19751468
the properties and large-scale production of l-asparaginase from citrobacter.an intracellular l-asparaginase with antitumour activity was purified from a strain of citrobacter. the optimum conditions for enzyme production by fermentation on scales up to 2700 l were investigated. highest enzyme yield was obtained in corn-steep liquor medium (9-2%, w/v) at 37 degrees c. oxygen limitation was not necessary for high enzyme yield. a total recovery of 4-3% from nucleic-acid-free extract and a 180-fold increase in specific activity were obtained after purificaiton. the specific ...1975465
Displaying items 3601 - 3617 of 3617