Publications

china: from sars to the stars.		2003	14685192
breakthrough of the year. sars: a pandemic prevented.		2003	14684791
[gene prediction and function research of sars-cov(bj01)].	through reading the articles, this study points out the shortage of gene prediction and function research about sars-cov, and predict it again for developing effective drugs and future vaccines. using twelve gene prediction methods to predict coronavirus known genes, we select four better methods including heuristic models, gene identification, zcurve_cov and orf finder to predict sars-cov(bj01), and use atgpr for analyzing probability of initiation codon and kozak rule, search transcription reg ...	2003	14682248
estimated timing of the last common ancestor of the sars coronavirus.		2003	14681521
detection of sars coronavirus in plasma by real-time rt-pcr.		2003	14681520
the severe acute respiratory syndrome.		2003	14681510
sars--one year later.		2003	14681502
database resources of the national center for biotechnology information: update.	in addition to maintaining the genbank(r) nucleic acid sequence database, the national center for biotechnology information (ncbi) provides data analysis and retrieval resources for the data in genbank and other biological data made available through ncbi's website. ncbi resources include entrez, pubmed, pubmed central, locuslink, the ncbi taxonomy browser, blast, blast link (blink), electronic pcr, orffinder, spidey, refseq, unigene, homologene, protest, dbmhc, dbsnp, cancer chromosome aberrati ...	2004	14681353
[epidemiological study of the features of the index cases in sars case clusters].	to understand the epidemiological characteristics of the index cases in severe acute respiratory syndrome (sars) cases clusters and to evaluate their importance in the whole epidemic process of sars.	2003	14678890
emerging viruses set to soar.	the emergence rate of novel viruses, such as the coronavirus that sparked sars, could well be on the rise. researchers now think that the sars virus split from group 2 coronaviruses, and that this happened relatively recently on the scale of coronavirus evolution.	2003	14678728
a wake-up call for our health-care system.		2003	14677347
sars.		2003	14677346
severe acute respiratory syndrome vaccine development: experiences of vaccination against avian infectious bronchitis coronavirus.	vaccines against infectious bronchitis of chickens (gallus gallus domesticus) have arguably been the most successful, and certainly the most widely used, of vaccines for diseases caused by coronaviruses, the others being against bovine, canine, feline and porcine coronaviruses. infectious bronchitis virus (ibv), together with the genetically related coronaviruses of turkey (meleagris gallopovo) and ring-necked pheasant (phasianus colchicus), is a group 3 coronavirus, severe acute respiratory syn ...	2003	14676007
severe acute respiratory syndrome vaccine development: experiences of vaccination against avian infectious bronchitis coronavirus.	vaccines against infectious bronchitis of chickens (gallus gallus domesticus) have arguably been the most successful, and certainly the most widely used, of vaccines for diseases caused by coronaviruses, the others being against bovine, canine, feline and porcine coronaviruses. infectious bronchitis virus (ibv), together with the genetically related coronaviruses of turkey (meleagris gallopovo) and ring-necked pheasant (phasianus colchicus), is a group 3 coronavirus, severe acute respiratory syn ...	2003	14676007
severe acute respiratory syndrome (sars)--paradigm of an emerging viral infection.	an acute and often severe respiratory illness emerged in southern china in late 2002 and rapidly spread to different areas of the far east as well as several countries around the globe. when the outbreak of this apparently novel infectious disease termed severe acute respiratory syndrome (sars) came to an end in july 2003, it had caused over 8000 probable cases worldwide and more than 700 deaths. starting in march 2003, the world health organization (who) organised an unprecedented international ...	2004	14675864
the secret life of ace2 as a receptor for the sars virus.	the membrane-associated carboxypeptidase angiotensin-converting enzyme 2 (ace2) is an essential regulator of heart function. now, li at al. identify and characterize an unexpected second function of ace2 as a partner of the sars-cov spike glycoprotein in mediating virus entry and cell fusion.	2003	14675530
[expression, purification and identification of recombinant sars coronavirus membrane protein].	a novel coronavirus (sars-coronavirus, sars-cov) was discovered as the pathogen of the severe acute respiratory syndrome (sars). according to studies with other coronaviruses, the membrane protein (m protein) is the main structural protein and the recombinant m protein may be useful as an antigen for detecting antibodies against coronavirus and for preparing vaccine. in this work, the m protein of sars-cov was expressed in e. coli as fusion protein with maltose binding protein at n-terminus and ...	2003	14673508
systematic, genome-wide identification of host genes affecting replication of a positive-strand rna virus.	positive-strand rna viruses are the largest virus class and include many pathogens such as hepatitis c virus and the severe acute respiratory syndrome coronavirus (sars). brome mosaic virus (bmv) is a representative positive-strand rna virus whose rna replication, gene expression, and encapsidation have been reproduced in the yeast saccharomyces cerevisiae. by using traditional yeast genetics, host genes have been identified that function in controlling bmv translation, selecting bmv rnas as rep ...	2003	14671320
mosaic evolution of the severe acute respiratory syndrome coronavirus.	severe acute respiratory syndrome (sars) is a deadly form of pneumonia caused by a novel coronavirus, a viral family responsible for mild respiratory tract infections in a wide variety of animals including humans, pigs, cows, mice, cats, and birds. analyses to date have been unable to identify the precise origin of the sars coronavirus. we used bayesian, neighbor-joining, and split decomposition phylogenetic techniques on the sars virus replicase, surface spike, matrix, and nucleocapsid proteins ...	2004	14671089
a 193-amino acid fragment of the sars coronavirus s protein efficiently binds angiotensin-converting enzyme 2.	the coronavirus spike (s) protein mediates infection of receptor-expressing host cells and is a critical target for antiviral neutralizing antibodies. angiotensin-converting enzyme 2 (ace2) is a functional receptor for the coronavirus (severe acute respiratory syndrome (sars)-cov) that causes sars. here we demonstrate that a 193-amino acid fragment of the s protein (residues 318-510) bound ace2 more efficiently than did the full s1 domain (residues 12-672). smaller s protein fragments, expressin ...	2004	14670965
revised u.s. surveillance case definition for severe acute respiratory syndrome (sars) and update on sars cases--united states and worldwide, december 2003.	during the 2003 epidemic of severe acute respiratory syndrome (sars), cdc and the council of state and territorial epidemiologists (cste) developed surveillance criteria to identify persons with sars. the surveillance case definition changed throughout the epidemic as understanding of the clinical, laboratory, and transmission characteristics of sars-associated coronavirus (sars-cov) increased. on june 26, cste adopted a position statement to add sars-cov disease to the national notifiable disea ...	2003	14668711
severe acute respiratory syndrome and influenza: virus incursions from southern china.		2003	14668255
effects of a sars-associated coronavirus vaccine in monkeys.	the causative agent of severe acute respiratory syndrome (sars) has been identified as a new type of coronavirus. here, we have investigated the ability of adenoviral delivery of codon-optimised sars-cov strain urbani structural antigens spike protein s1 fragment, membrane protein, and nucleocapsid protein to induce virus-specific broad immunity in rhesus macaques. we immunised rhesus macaques intramuscularly with a combination of the three ad5-sars-cov vectors or a control vector and gave a boo ...	2003	14667748
diagnosis of severe acute respiratory syndrome (sars) by detection of sars coronavirus nucleocapsid antibodies in an antigen-capturing enzyme-linked immunosorbent assay.	recombinant severe acute respiratory syndrome (sars) coronavirus nucleocapsid protein was employed to establish an antigen-capturing enzyme-linked immunosorbent assay (elisa). antinucleocapsid protein antibodies could be detected in 68.4% of probable sars patients 6 to 10 days after illness and in 89.6% of the patients 11 to 61 days after illness. no false-positive results were observed in 20 non-sars fever patients, 24 non-sars respiratory illness patients, and 20 health care workers. among 940 ...	2003	14662982
my experience with sars.		2003	14662665
the new normal: a sars diary.		2003	14662664
emerging viral infections in a rapidly changing world.	emerging viral infections in both humans and animals have been reported with increased frequency in recent years. recent advances have been made in our knowledge of some of these, including severe acute respiratory syndrome-associated coronavirus, influenza a virus, human metapneumovirus, west nile virus and ebola virus. research efforts to mitigate their effects have concentrated on improved surveillance and diagnostic capabilities, as well as on the development of vaccines and antiviral agents ...	2003	14662395
a clinicopathological study of three cases of severe acute respiratory syndrome (sars).	the severe acute respiratory syndrome (sars) caused a large outbreak of atypical pneumonia in beijing, china from early march 2003. we report the pathological features from three patients who died of sars.	2003	14660106
implications of unrecognized severe acute respiratory syndrome.		2003	14657739
[origin of sars from accelerated evolution of a virus by combined pollution].	the sars virus origin which obviously differs from the theory of out-space origin and the theory of wildlife origin was theoretically discussed based on principles of pollution-evolution ecology, pathogenesis and infectant characteristics of sars, and some relevant experimental evidence. it emphasizes on the fact that increasingly combined pollution in china provides a substantial basis for virus evolution. gene recombination and fusion of more than one virus in ecosystems should take charge wit ...	2003	14655379
molecular model of sars coronavirus polymerase: implications for biochemical functions and drug design.	the causative agent of severe acute respiratory syndrome (sars) is a previously unidentified coronavirus, sars-cov. the rna-dependent rna polymerase (rdrp) of sars-cov plays a pivotal role in viral replication and is a potential target for anti-sars therapy. there is a lack of structural or biochemical data on any coronavirus polymerase. to provide insights into the structure and function of sars-cov rdrp, we have located its conserved motifs that are shared by all rdrps, and built a three-dimen ...	2003	14654687
genomic characterisation of the severe acute respiratory syndrome coronavirus of amoy gardens outbreak in hong kong.	severe acute respiratory syndrome (sars) is a global health concern. in hong kong, two major outbreaks, one hospital based and the other in the amoy gardens apartments, were identified. the frequency of diarrhoea, admission to intensive care, and mortality differed significantly between the two outbreaks. we did genomic sequencing for viral isolates from five amoy gardens patients. the virus sequence was identical in four of these five patients. the sequence data from one hospital case and the f ...	2003	14654320
molecular diagnostics of atypical pneumonia.	the emergence of nucleic acid-based molecular techniques has significantly enhanced laboratory diagnosis and monitoring of atypical pneumonia. these techniques have not only provided rapid and sensitive detection of fastidious microbial organisms but have also played critical roles in identifying and characterizing emerging pathogens that cause atypical pneumonia. other benefits that molecular techniques can bring to the field include organism differentiation, quantitation, typing, and antibioti ...	2003	14653964
a real-time pcr for sars-coronavirus incorporating target gene pre-amplification.	an enhanced polymerase chain reaction (pcr) assay to detect the coronavirus associated with severe acute respiratory syndrome (sars-cov) was developed in which a target gene pre-amplification step preceded taqman real-time fluorescent pcr. clinical samples were collected from 120 patients diagnosed as suspected or probable sars cases and analyzed by conventional pcr followed by agarose gel electrophoresis, conventional taqman real-time pcr, and our enhanced taqman real-time pcr assays. an amplic ...	2003	14652014
the sars-cov s glycoprotein: expression and functional characterization.	we have cloned, expressed, and characterized the full-length and various soluble fragments of the sars-cov (tor2 isolate) s glycoprotein. cells expressing s fused with receptor-expressing cells at neutral ph suggesting that the recombinant glycoprotein is functional, its membrane fusogenic activity does not require other viral proteins, and that low ph is not required for triggering membrane fusion; fusion was not observed at low receptor concentrations. s and its soluble ectodomain, s(e), were ...	2003	14651994
[reliability of detecting sars-cov antibody for diagnosis of sars].	to discuss the reliability of sars-cov antibody detection for sars diagnosis.	2003	14650153
[isolation and identification of sars-coronavirus in nasal and throat swabs collected from clinically diagnosed sars patients].	to isolate and identify sars-coronavirus in nasal and throat swabs collected from clinically diagnosed severe acute respiratory syndrome (sars) patients.	2003	14650149
[cloning, expression and purification of sars coronavirus pumc2 strain nucleocapsid protein].	to clone, express and purify nucleocapsid protein from sars coronavirus pumc2 strain.	2003	14650145
[cdnas cloning of sars-cov pumc2 viral genome].	to get the cdna clones which cover the whole genome of sars-cov pumc2 strain.	2003	14650144
[analysis on the sars-cov genome of pumc01 isolate].	to perform variation and phylogenetics analysis on the sars-cov genome sequence (pumc01) isolated in the peking union medical college hospital.	2003	14650143
[sars-cov: 2. modeling sars epidemic].		2003	14648488
angiotensin-converting enzyme 2 is a functional receptor for the sars coronavirus.	spike (s) proteins of coronaviruses, including the coronavirus that causes severe acute respiratory syndrome (sars), associate with cellular receptors to mediate infection of their target cells. here we identify a metallopeptidase, angiotensin-converting enzyme 2 (ace2), isolated from sars coronavirus (sars-cov)-permissive vero e6 cells, that efficiently binds the s1 domain of the sars-cov s protein. we found that a soluble form of ace2, but not of the related enzyme ace1, blocked association of ...	2003	14647384
proliferative growth of sars coronavirus in vero e6 cells.	an isolate of sars coronavirus (strain 2003va2774) was obtained from a patient and used to infect vero e6 cells. the replication cycle of the virus was followed from 1 to 30 h post-infection (p.i.). it was surprising to observe the swift growth of this human virus in vero cells. within the first hour of infection, the most obvious ultrastructural change was the proliferation of the golgi complexes and related vesicles accompanied by swelling of some of the trans-golgi sacs. extracellular virus p ...	2003	14645910
phylogeny of the sars coronavirus.		2003	14645828
inhibition of sars-associated coronavirus infection and replication by rna interference.		2003	14645307
sars infection among health care workers in beijing, china.		2003	14645305
detection of sars coronavirus rna in the cerebrospinal fluid of a patient with severe acute respiratory syndrome.		2003	14633896
serial analysis of the plasma concentration of sars coronavirus rna in pediatric patients with severe acute respiratory syndrome.		2003	14633884
assessment of immunoreactive synthetic peptides from the structural proteins of severe acute respiratory syndrome coronavirus.	the widespread threat of severe acute respiratory syndrome (sars) to human life has spawned challenges to develop fast and accurate analytical methods for its early diagnosis and to create a safe antiviral vaccine for preventive use. consequently, we thoroughly investigated the immunoreactivities with patient sera of a series of synthesized peptides from sars-coronavirus structural proteins.	2003	14633869
quantitative analysis and prognostic implication of sars coronavirus rna in the plasma and serum of patients with severe acute respiratory syndrome.	the availability of an early diagnostic tool for severe acute respiratory syndrome (sars) would have major public health implications. we investigated whether the sars coronavirus (sars-cov) can be detected in serum and plasma samples during the early stages of sars and studied the potential prognostic implications of such an approach.	2003	14633867
[cardiac arrest in severe acute respiratory syndrome: analysis of 15 cases].	to investigate the causes for cardiac arrest in severe acute respiratory syndrome (sars) patients.	2003	14633442
[the first case of severe acute respiratory syndrome in foshan].	to evaluate the epidemiologic feature, diagnosis and treatment of severe acute respiratory syndrome (sars).	2003	14633441
[dynamic changes and the meanings of blood cytokines in severe acute respiratory syndrome].	to investigate the dynamic changes observed in serum levels of interleukins (ils), tumor necrosis factor-alpha (tnf-alpha) and transforming growth factor-beta1 (tgf-beta1) in severe acute respiratory syndrome (sars).	2003	14633438
[the quantitative detection of anti-coronavirus antibody titer in medical personnel closely contacted with severe acute respiratory syndrome patients].	to study the serum anti-coronavirus antibody titer in medical personnel who had closely contacted with severe acute respiratory syndrome (sars) patients.	2003	14633437
potential of zoonotic transmission of non-primate foamy viruses to humans.	the zoonotic introduction of an animal pathogen into the human population and the subsequent extension or alteration of its host range leading to the successful maintenance of the corresponding pathogen by human-to-human transmission pose a serious risk for world-wide health care. such a scenario occurred for instance by the introduction of simian immunodeficiency viruses into the human population resulting in the human immunodeficiency viruses (hiv) and the subsequent aids pandemic or the propo ...	2003	14633194
implications of sars: medical geography and surveillance in disease detection.	to expose pharmacy educators and practitioners to concepts of medical geography and medical surveillance. severe acute respiratory syndrome (sars) is used as a case example because it is an emerging infection and a prime example of the type of disease that pharmacists may encounter in daily practice (e.g., easily transmitted, resembles the common cold).	2003	14632586
stability of sars coronavirus in human specimens and environment and its sensitivity to heating and uv irradiation.	the causal agent for sars is considered as a novel coronavirus that has never been described both in human and animals previously. the stability of sars coronavirus in human specimens and in environments was studied.	2003	14631830
sars virus: the beginning of the unraveling of a new coronavirus.	severe acute respiratory syndrome (sars) virus caused a severe outbreak in several regions of the world in 2003. the virus is a novel coronavirus, which may have an origin in wild animals such as civet cats in southern china. its genome structure, gene expression pattern and protein profiles are similar to those of other coronaviruses. however, distinct patterns of several open reading frames in the sars virus genome may contribute to its severe virulence. the potential mutability of the coronav ...	2003	14631105
severe acute respiratory syndrome (sars) in children: epidemiology, presentation and management.	severe acute respiratory syndrome (sars) is a newly recognised and highly contagious respiratory infection caused by a new strain of coronavirus. the disease can result in progressive respiratory failure in adults and the mortality rate has been reported to be 8-15%. this infection spreads by droplet transmission and children appear to acquire sars through close household contact exposure to infected adults. disease severity is, however, much milder in the paediatric age group. the common labora ...	2003	14629957
air pollution and case fatality of sars in the people's republic of china: an ecologic study.	severe acute respiratory syndrome (sars) has claimed 349 lives with 5,327 probable cases reported in mainland china since november 2002. sars case fatality has varied across geographical areas, which might be partially explained by air pollution level.	2003	14629774
[severe acute respiratory syndrome--challenging and conquering].		2003	14628356
[expression of the monoclonal antibody against nucleocapsid antigen of sars-associated coronavirus in autopsy tissues from sars patients].	to investigate the presence and distribution of severe acute respiratory syndrome (sars)-associated coronavirus (sars-cov) in autopsy tissues obtained from patients died of sars.	2003	14625168
[detection of severe acute respiratory syndrome (sars)-associated coronavirus rna in autopsy tissues with in situ hybridization].	to explore the distribution of severe acute respiratory syndrome (sars)-associated coronavirus (sars-cov) in sars autopsy tissues at the molecular level.	2003	14625166
viral discovery and sequence recovery using dna microarrays.	because of the constant threat posed by emerging infectious diseases and the limitations of existing approaches used to identify new pathogens, there is a great demand for new technological methods for viral discovery. we describe herein a dna microarray-based platform for novel virus identification and characterization. central to this approach was a dna microarray designed to detect a wide range of known viruses as well as novel members of existing viral families; this microarray contained the ...	2003	14624234
potential for antiviral treatment of severe acute respiratory syndrome.	severe acute respiratory syndrome is a new, sometimes lethal disease of humans that is caused by a novel coronavirus. to date there have been over 750 related deaths and there is clearly an urgent need to develop specific antiviral drugs to combat this disease. in this review, the authors shall focus on the molecular biology of the coronavirus and suggest how this information can be used to identify possible targets for antiviral drugs.	2003	14624107
activation of ap-1 signal transduction pathway by sars coronavirus nucleocapsid protein.	in march 2003, a novel coronavirus was isolated from patients exhibiting atypical pneumonia and subsequently proven to be the causative agent of the disease now referred to as severe acute respiratory syndrome (sars). the complete genome of the sars coronavirus (sars-cov) has since been sequenced. the sars-cov nucleocapsid (sars-cov n) shares little homology with other members of the coronavirus family. to determine if the n protein is involved in the regulation of cellular signal transduction, ...	2003	14623261
assessment of putative protein targets derived from the sars genome.	the ability to rapidly and reliably develop hypotheses on the function of newly discovered protein sequences requires systematic and comprehensive analysis. such an analysis, embodied within the ds geneatlas pipeline, has been used to critically evaluate the severe acute respiratory syndrome (sars) genome with the goal of identifying new potential targets for viral therapeutic intervention. this paper discusses several new functional hypotheses on the roles played by the constituent gene product ...	2003	14623076
vitamin c and sars coronavirus.		2003	14613951
characterizing degradation products of peptides containing n-terminal cys residues by (off-line high-performance liquid chromatography)/matrix-assisted laser desorption/ionization quadrupole time-of-flight measurements.	a transformation analogous to the well-known conversion of an n-terminal glutamine residue to pyroglutamic acid is the cyclization of an n-terminal carboxyamidomethylated cysteine residue (the normal product of alkylation with iodoacetamide). this yields 5-oxothiomorpholine-3-carboxylic acid, with the same 17 da mass loss observed in the gln reaction. nineteen tryptic peptides with cys at the n-terminal were identified for this study, and compared with eight with n-terminal gln. when examined by ...	2003	14608624
severe acute respiratory distress syndrome (sars): a critical care perspective.	to review the epidemiology, clinical features, etiology, diagnosis, and management of severe acute respiratory syndrome (sars) from a critical care perspective.	2003	14605542
molecular epidemiology of sars--from amoy gardens to taiwan.		2003	14602893
the impact of community psychological responses on outbreak control for severe acute respiratory syndrome in hong kong.	to examine the public's knowledge and perception of sars and the extent to which various precautionary measures have been adopted.	2003	14600110
sars: a new infectious disease for a new century.	a new infectious disease swept the world in early 2003, causing restrictions on international travel and economic downturn in affected countries.	2003	14596069
taiwanese scientists find genetic link to sars.		2003	14595411
[sars-cov: 1. the virus].		2003	14593622
infectious diseases. unexplained false alarm may hold lessons.		2003	14593143
infectious diseases. search for sars origins stalls.		2003	14593142
identifying inhibitors of the sars coronavirus proteinase.	the severe acute respiratory syndrome (sars) is a serious respiratory illness that has recently been reported in parts of asia and canada. in this study, we use molecular dynamics (md) simulations and docking techniques to screen 29 approved and experimental drugs against the theoretical model of the sars cov proteinase as well as the experimental structure of the transmissible gastroenteritis virus (tgev) proteinase. our predictions indicate that existing hiv-1 protease inhibitors, l-700,417 fo ...	2003	14592491
[what have we learned about the coronaviruses in animals?].		2003	14587440
[sars--severe acute respiratory syndrome--current knowledge].		2003	14587434
[severe acute respiratory syndrome].		2003	14587416
virology: sars virus infection of cats and ferrets.		2003	14586458
the crystal structures of severe acute respiratory syndrome virus main protease and its complex with an inhibitor.	a newly identified severe acute respiratory syndrome coronavirus (sars-cov), is the etiological agent responsible for the outbreak of sars. the sars-cov main protease, which is a 33.8-kda protease (also called the 3c-like protease), plays a pivotal role in mediating viral replication and transcription functions through extensive proteolytic processing of two replicase polyproteins, pp1a (486 kda) and pp1ab (790 kda). here, we report the crystal structures of the sars-cov main protease at differe ...	2003	14585926
epidemiology and cause of severe acute respiratory syndrome (sars) in guangdong, people's republic of china, in february, 2003.	an epidemic of severe acute respiratory syndrome (sars) has been associated with an outbreak of atypical pneumonia originating in guangdong province, people's republic of china. we aimed to identify the causative agent in the guangdong outbreak and describe the emergence and spread of the disease within the province.	2003	14585636
[genomic variations in the locus for aminopeptidase n:a putative cellular receptor for sars-cov spike glycoprotein].	aminopeptidase n has been identified as the cellular receptor for human coronavirus hcov-229e and was a putative receptor for the spike glycoprotein encoded by the sars-associated coronavirus (sars-cov). we report here identification of 9 single nucleotide polymorphisms (snps) in anpep, encoding human aminopeptidase n, in chinese. all anpep exons and their flanking intronic sequences were amplified from unrelated normal individuals by polymerase chain reaction (pcr) and screened using denaturing ...	2003	14579541
how sars changed the world in less than six months.		2003	14576896
[public health and severe acute respiratory syndrome].		2003	14576858
[detection of the anti-sars-coronavirus specific antibody levels in 156 sars patients].	the objective of this study was to explore the development of igg and igm against sars cov and characteristics of changes of antibody titers in patients with severe acute respiratory syndrome (sars) and to search the opportunity for collecting specific anti-serum from convalescent patients with sars. the anti-sars-coronavirus specific antibody levels in 156 sars patients were measured with elisa. the results showed that the total positive rates of igg and igm were 75.6% and 41.7% respectively, a ...	2003	14575532
[analysis of false-positive associated with antibody tests for sars-cov in sle patients].	to discuss the false-positive of serological diagnostic testing for coronavirus antibody in patients with systemic lupus erythematosus(sle), 66 normal individual and 31 sle with non-sars patients were detected for sars-associated coronavirus (sars-cov) antibody and rna by enzymelinked immunosorbent assays(elisa) and reverse transcriptase-polymerase chain reaction(rt-pcr). the result showed 2/66 cases(3.0%) were positive of sars-cov-igg antibody and 66 cases were negative of sars-cov-igm antibody ...	2003	14574997
prediction of proteinase cleavage sites in polyproteins of coronaviruses and its applications in analyzing sars-cov genomes.	recently, we have developed a coronavirus-specific gene-finding system, zcurve_cov 1.0. in this paper, the system is further improved by taking the prediction of cleavage sites of viral proteinases in polyproteins into account. the cleavage sites of the 3c-like proteinase and papain-like proteinase are highly conserved. based on the method of traditional positional weight matrix trained by the peptides around cleavage sites, the present method also sufficiently considers the length conservation ...	2003	14572668
studies explore impact of new pathogens: investigators report on metapneumovirus, sars.		2003	14570932
report details lessons from sars outbreak.		2003	14570038
reverse genetics with a full-length infectious cdna of severe acute respiratory syndrome coronavirus.	a previously undescribed coronavirus (cov) is the etiologic agent responsible for severe acute respiratory syndrome (sars). using a panel of contiguous cdnas that span the entire genome, we have assembled a full-length cdna of the sars-cov urbani strain, and have rescued molecularly cloned sars viruses (infectious clone sars-cov) that contained the expected marker mutations inserted into the component clones. recombinant viruses replicated as efficiently as wt virus and both were inhibited by tr ...	2003	14569023
[isolation and identification of the infective agent of severe acute respiratory syndrome (sars) from a patient with atypical pneumonia].	the virological, morphological, molecular biological and immunochemical study of the infective agent isolated from the patient with the symptoms of atypical pneumonia, hospitalized in the infectious department of the clinical hospital in blagoveshchensk, was carried out. thus the fact of the appearance of sars virus on the territory of russia was proved. the isolated infective agent, identified as coronavirus strain cod, was partly characterized and deposited to the virus collection of the cente ...	2003	14565137
prevalence of igg antibody to sars-associated coronavirus in animal traders--guangdong province, china, 2003.	severe acute respiratory syndrome (sars) was identified in 2003 as an infectious disease caused by the sars-associated coronavirus (sars-cov), a member of the coronavirus family not observed previously in humans. because its sequence data differ from that of known human coronaviruses, sars-cov is suspected to have crossed the species barrier between an animal host and humans. the sars outbreak began in china's guangdong province, where approximately 1,500 probable cases were identified during no ...	2003	14561956
prevalence of igg antibody to sars-associated coronavirus in animal traders--guangdong province, china, 2003.	severe acute respiratory syndrome (sars) was identified in 2003 as an infectious disease caused by the sars-associated coronavirus (sars-cov), a member of the coronavirus family not observed previously in humans. because its sequence data differ from that of known human coronaviruses, sars-cov is suspected to have crossed the species barrier between an animal host and humans. the sars outbreak began in china's guangdong province, where approximately 1,500 probable cases were identified during no ...	2003	14561956
biosynthesis, purification, and substrate specificity of severe acute respiratory syndrome coronavirus 3c-like proteinase.	the 3c-like proteinase of severe acute respiratory syndrome (sars) coronavirus has been proposed to be a key target for structural-based drug design against sars. in order to understand the active form and the substrate specificity of the enzyme, we have cloned, expressed, and purified sars 3c-like proteinase. analytic gel filtration shows a mixture of monomer and dimer at a protein concentration of 4 mg/ml and mostly monomer at 0.2 mg/ml, which correspond to the concentration used in the enzyme ...	2004	14561748
[detection and analysis of sars coronavirus-specific antibodies in sera from non-sars children].	to examine the presence of severe acute respiratory syndrome (sars) coronavirus-specific antibodies in the sera from non-sars children.	2003	14559701
canada's approach to public health must be reinvented: sars report.		2003	14557334
new sars recommendations to be released this month.		2003	14557328