Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| characterization of beet necrotic yellow vein furovirus from spanish sugar beets. | rhizomania is a viral disease, caused by beet necrotic yellow vein furovirus (bnyvv), which was detected in spanish sugar beets in 1988, it being focused on the castilla y león region. bnyvv has five rna fragments with specific functions, and the different composition and proportion of rna in the virions allow their separation and the characterization of their activities during the development of the disease. thirty-six samples of sugar beet rootlets and frozen pulps from three different sugar b ... | 1999 | 10943398 |
| rhizoscan: a semiautomatic image processing system for characterization of the morphology and secondary metabolite concentration in hairy root cultures. | we describe the application of the newly developed rhizoscan software for accurate morphological analysis and determination of overall and local secondary metabolite concentrations in two clones of beta vulgaris throughout the growth period. local secondary metabolite concentrations may be determined at any point in the root, and pigment gradients in each lateral root can be followed during culture and saved to the computer. throughout the entire analysis process, an image appears in a graphical ... | 2000 | 10940859 |
| optimisation of culture medium and conditions for alpha-l-arabinofuranosidase production by the extreme thermophilic eubacterium rhodothermus marinus. | the culture medium for rhodothermus marinus was optimised on a shake-flask scale by using statistical factorial designs for enhanced production of a highly thermostable alpha-l-arabinofuranosidase (afase). the medium containing 3.6 g/l birch wood xylan and 8.2 g/l yeast extract yielded a maximum of 110 nkat/ml afase activity together with 125 nkat/ml xylanase and 65 nkat/ml beta-xylosidase activity. in addition, low levels of beta-mannanase (30 nkat/ml), alpha-galactosidase (0.2 nkat/ml), beta-g ... | 2000 | 10938421 |
| interaction between hsp70 homolog and filamentous virions of the beet yellows virus. | an hsp70 homolog (hsp70h), encoded by the closterovirus beet yellows virus (byv), functions in viral movement from cell to cell. a previous study revealed that in infected cells, hsp70h colocalizes with the masses of byv filamentous virions. here we demonstrate that hsp70h forms a physical complex with byv virions. this conclusion is based on both the comigration of hsp70h with byv virions in sucrose density gradients and the coimmunoprecipitation of the hsp70h and byv capsid protein using anti- ... | 2000 | 10936104 |
| overproduction of an inducible extracellular serine protease improves biological control of pythium ultimum by stenotrophomonas maltophilia strain w81. | stenotrophomonas maltophilia w81 can protect sugar beet against pythium:-mediated damping-off disease through the production of an extracellular protease. here, the proteolytic enzyme of w81 was purified by anion-exchange chromatography and characterized as a serine protease. the purified enzyme was fungicidal against pythium: ultimum in vitro. its synthesis was inducible by casein in w81, and mutagenesis of this strain using the luciferase (luxab) reporter transposon tn5-764cd resulted in the i ... | 2000 | 10931911 |
| purification and characterization of a novel alpha-l-arabinofuranosidase from pichia capsulata x91. | an intracellular alpha-l-arabinofuranosidase from pichia capsulata x91 was purified and characterized. the enzyme was purified to homogeneity from a cell-free extract by ammonium sulfate treatment, concanavalin a-sepharose, ion-exchange chromatography with deae bio-gel a agarose, arabinose-sepharose 6b affinity chromatography, and hydroxyapatite column chromatography. the apparent molecular mass of the enzyme was estimated to be 250 kda by native-page. the enzyme molecule was suggested to be a t ... | 2000 | 10923788 |
| atp-induced sucrose efflux from red-beet tonoplast vesicles. | sucrose efflux from the vacuole of mobilizing red-beet (beta vulgaris l.) hypocotyl cells was investigated using purified tonoplast vesicles. tonoplast vesicle purity was assured by the immunoreactivity to antibodies raised against the vacuolar atpase and by the strong inhibition exhibited by the h+-atpase to bafilomycin-a and no3-. inhibition of the h+-atpase by vanadate and azide was negligible. sucrose was loaded into tonoplast vesicles by using the ph-jump method of energization. addition of ... | 2000 | 10923706 |
| simultaneous detection of the establishment of seed-inoculated pseudomonas fluorescens strain dr54 and native soil bacteria on sugar beet root surfaces using fluorescence antibody and in situ hybridization techniques. | colonization at sugar beet root surfaces by seedling-inoculated biocontrol strain pseudomonas fluorescens dr54 and native soil bacteria was followed over a period of 3 weeks using a combination of immunofluorescence (dr54-targeting specific antibody) and fluorescence in situ hybridization (rrna-targeting eubacteria eub338 probe) techniques with confocal laser scanning microscopy. the dual staining protocol allowed cellular activity (ribosomal number) to be recorded in both single cells and micro ... | 2000 | 10922498 |
| [the actin cytoskeletal characteristics of the root meristem cells in beta vulgaris l. at different mitotic stages]. | for the first time the actin cytoskeleton in meristem cells of b. vulgaris root has been studied. it was established that all discovered earlier patterns of microfilament arrangement were also specific for meristem of present species. specific rearrangements of actin in the sites of spindle (prophase) and phragmoplast formation (telophase) were revealed. it is supposed, that actin cytoskeleton determines certain position of spindle in cells and takes part in the cell plate formation. | 2008 | 10920855 |
| fish to mitotic chromosomes and extended dna fibres of beta procumbens in a series of monosomic additions to beet (b. vulgaris). | the physical localization and organization of a procumbentes-specific repetitive dna sequence, pb6-4, on the chromosomes of beta procumbens (2n = 18) were studied, using fish (fluorescence in situ hybridization) to mitotic chromosomes and extended dna fibres. the chromosomes of b. procumbens were studied in metaphase complements of the species itself, as well as in preparations of a series of eight different b. procumbens-derived monosomic additions to b. vulgaris (2n = 18). fish to chromosome s ... | 2000 | 10919719 |
| culture of red beet hairy roots by considering variation in sensitivity of tip meristems to hydraulic stress. | in the culture of red beet hairy roots in shaking flasks, a period for acclimation without lateral root generation existed at the early stage, and the root tip meristems containing growing points (gps) were found to be damaged under an elevated shear stress condition. the loading experiments of shear stress to the hairy roots revealed that the gps subjected to the acclimation acquired tolerance to shear stress, retaining relatively high viability of gps up to 0.6n/m(2) of loaded shear stress. ne ... | 2000 | 10908861 |
| the effect of red beet (beta vulgaris var. rubra) fiber on alimentary hypercholesterolemia and chemically induced colon carcinogenesis in rats. | the effect of diet supplemented with 5% and 15% cellulose or with 15% fiber isolated from red beet (beta vulgaris var. rubra) on the development of alimentary hypercholesterolemia and chemically induced colon carcinoma was studied in male wistar rats. hypercholesterolemia was induced by a diet containing 0.3% of cholesterol and colon carcinoma was induced by treatment with dimethylhydrazine (20 mg/kg, 12 doses applied s.c. in one-week intervals). fibrous matter isolated from red beet contained 8 ... | 2000 | 10907240 |
| enhancement in activity of homologous and heterologous baculoviruses infectious to beet armyworm (lepidoptera: noctuidae) by an optical brightener. | the nuclear polyhedrosis virus (npv) from the beet armyworm, spodoptera exigua (hübner) (semnpv), was the most active virus tested against the beet armyworm (lc50 = 4.1 pibs/mm2), followed by nuclear polyhedrosis viruses from the alfalfa looper, autographa californica (speyer) (acmnpv; lc50 = 92.6 pibs/mm2), and the celery looper, anagrapha falcifera (kirby) (afmnpv; lc50 = 195.7 pibs/mm2). in the case of the nuclear polyhedrosis virus from the bollworm, helicoverpa armigera (hübner), lc50s coul ... | 2000 | 10902301 |
| monitoring and control of industrial downstream processing of sugar beet molasses. | in present work the determination of several amino acids during the industrial chromatographic desugarisation of molasses is presented. the use of innovative biosensor systems for highly specific detection of serine is described. using two-dimensional fluorescence spectrometry, a non-invasive method for the determination of several product fractions could be established in an industrial chromatographic procedure. | 2000 | 10895955 |
| calcium release from the endoplasmic reticulum of higher plants elicited by the nadp metabolite nicotinic acid adenine dinucleotide phosphate. | higher plants share with animals a responsiveness to the ca(2+) mobilizing agents inositol 1,4,5-trisphosphate (insp(3)) and cyclic adp-ribose (cadpr). in this study, by using a vesicular (45)ca(2+) flux assay, we demonstrate that microsomal vesicles from red beet and cauliflower also respond to nicotinic acid adenine dinucleotide phosphate (naadp), a ca(2+)-releasing molecule recently described in marine invertebrates. naadp potently mobilizes ca(2+) with a k(1/2) = 96 nm from microsomes of non ... | 2000 | 10890899 |
| codominant analysis of banding data from a dominant marker system by normal mixtures. | amplified fragment length polymorphisms (aflps) currently are among the most widely used marker systems. in many studies, aflps are analyzed on the basis of the presence or absence of a band on an electrophoretic gel. as a result, dominant homozygous individuals are not distinguished from heterozygous individuals, resulting in a considerable loss of information. this article shows how codominant information can be obtained if the amount of pcr products is quantified. due to measurement variation ... | 2000 | 10880503 |
| the complete nucleotide sequence of the mitochondrial genome of sugar beet (beta vulgaris l.) reveals a novel gene for trna(cys)(gca). | we determined the complete nucleotide sequence of the mitochondrial genome of an angiosperm, sugar beet (beta vulgaris cv tk81-o). the 368 799 bp genome contains 29 protein, five rrna and 25 trna genes, most of which are also shared by the mitochondrial genome of arabidopsis thaliana, the only other completely sequenced angiosperm mitochondrial genome. however, four genes identified here (namely rps13, trnf-gaa, ccb577 and trnc2-gca) are missing in arabidopsis mitochondria. in addition, four gen ... | 2000 | 10871408 |
| dietary soy protein is associated with reduced intestinal mucosal polyamine concentration in male wistar rats. | quantitation of polyamine levels has been correlated with biomarkers of proliferation in the colon mucosa where dysregulated epithelial hyperproliferation is associated with colorectal cancer risk. this study was performed to assess the response of polyamine measurements to dietary factors in an animal model. male wistar rats were fed purified diet or diets substituted by 20% lard fat, 20% beet fiber and 20% soy protein. after 2 wk, mucosal polyamines were measured along intestinal tracts by hpl ... | 2000 | 10867056 |
| dietary sugar beet fiber prevents the increase in aberrant crypt foci induced by gamma-irradiation in the colorectum of rats treated with an immunosuppressant. | we demonstrated recently that gamma-irradiation can induce aberrant crypt foci (acf) in the rat colorectum. the aim of this study was to evaluate the effect of dietary sugar beet fiber (sbf) on the distribution of the cd8(+) intraepithelial lymphocyte (iel) in the colorectum and on the number of gamma-irradiation-induced acf of rats administered anti-asialo gm1 (alpha agm1) as an immunosuppressant. wistar/st rats fed a fiber-free diet or the diet supplemented with sbf (100 g/kg diet) were admini ... | 2000 | 10867036 |
| oxalate oxidases and differentiating surface structure in wheat: germins. | oxalate oxidases (oxos) have been found to be concentrated in the surface tissues of wheat embryos and grains: germin is concentrated in root and leaf sheaths that surround germinated embryos; pseudogermin (oxo-psi) is concentrated in the epidermis and bracts that 'encircle' mature grains. most strikingly, the epidermal accumulation of oxo-psi was found to presage the transition of a delicate 'skin', similar to the fragile epidermis of human skin, into the tough shell (the miller's 'beeswing') t ... | 2000 | 10861243 |
| [anomalies in cytoskeletal microtubules in a mutant line of sugar beets]. | the morphological phenotype of meimutation acd (abnormal cytoskeleton dynamics) in beta vulgaris was analysed using a classic method of achromatic figure visualization (navashin fixative). the mutation demonstrates two different phenotypes under different growth conditions. the first variant is characterized by formation of chaotic network of microtubule bundles instead of two bipolar spindles at metaphase ii. such abnormality leads to the formation of polyads and monads. the second variant of p ... | 2000 | 10849932 |
| fermentations of pectin-rich biomass with recombinant bacteria to produce fuel ethanol. | pectin-rich residues from sugar beet processing contain significant carbohydrates and insignificant amounts of lignin. beet pulp was evaluated for conversion to ethanol using recombinant bacteria as biocatalysts. hydrolysis of pectin-rich residues followed by ethanolic fermentations by yeasts has not been productive because galacturonic acid and arabinose are not fermentable to ethanol by these organisms. the three recombinant bacteria evaluated in this study, escherichia coli strain ko11, klebs ... | 2000 | 10849785 |
| involvement of jasmonate- and salicylate-related signaling pathways for the production of specific herbivore-induced volatiles in plants. | we compared volatiles from lima bean leaves (phaseolus lunatus) infested by either beet armyworm (spodoptera exigua), common armyworm [mythimna (pseudaletia) separata], or two-spotted spider mite (tetranychus urticae). we also analyzed volatiles from the leaves treated with jasmonic acid (ja) and/or methyl salicylate (mesa). the volatiles induced by aqueous ja treatment were qualitatively and quantitatively similar to those induced by s. exigua or m. separata damage. furthermore, both s. exigua ... | 2000 | 10845451 |
| the dual function in virulence and host range restriction of a gene isolated from the ppath (ehg) plasmid of erwinia herbicola pv. gypsophilae. | the host range of the gall-forming bacterium erwinia herbicola pv. gypsophilae (ehg) is restricted to gypsophila whereas erwinia herbicola pv. betae (ehb) attacks beet as well as gypsophila. both pathovars contain an indigenous plasmid (ppath(ehg or ppath(ehb)) that harbors pathogenicity genes, including the hrp gene cluster. a cosmid library of ehg824-1 plasmid dna was mobilized into ehb4188 and the transconjugants were screened for pathogenicity on beet. one ehb transconjugant harboring the co ... | 2000 | 10830268 |
| fermentation of plant cell wall derived polysaccharides and their corresponding oligosaccharides by intestinal bacteria. | new types of nondigestible oligosaccharides were produced from plant cell wall polysaccharides, and the fermentation of these oligosaccharides and their parental polysaccharides by relevant individual intestinal species of bacteria was studied. oligosaccharides were produced from soy arabinogalactan, sugar beet arabinan, wheat flour arabinoxylan, polygalacturonan, and rhamnogalacturonan fraction from apple. all of the tested substrates were fermented to some extent by one or more of the individu ... | 2000 | 10820072 |
| p42 movement protein of beet necrotic yellow vein virus is targeted by the movement proteins p13 and p15 to punctate bodies associated with plasmodesmata. | cell-to-cell movement of beet necrotic yellow vein virus (bnyvv) is driven by a set of three movement proteins--p42, p13, and p15--organized into a triple gene block (tgb) on viral rna 2. the first tgb protein, p42, has been fused to the green fluorescent protein (gfp) and fusion proteins between p42 and gfp were expressed from a bnyvv rna 3-based replicon during virus infection. gfp-p42, in which the gfp was fused to the p42 n terminus, could drive viral cell-to-cell movement when the copy of t ... | 2000 | 10796018 |
| effects of superovulated heifer diet type and quantity on relative mrna abundances and pyruvate metabolism in recovered embryos. | this study investigated the effects of quantity and type of diet fed to superovulated donor heifers on molecular and metabolic indices of embryonic development. these effects included the relative abundances of mrnas for the alpha 1 subunit of na/k-atpase and the antioxidant enzyme cu/zn-sod, as well as pyruvate utilization in bovine morulae and blastocysts developed in vivo. heifers were fed a daily ration of either grass silage and a citrus-beet pulp-based concentrate or grass silage and a bar ... | 2000 | 10793627 |
| characterization of a new antifungal non-specific lipid transfer protein (nsltp) from sugar beet leaves. | a novel protein (iwf5) comprising 92 amino acids has been purified from the intercellular washing fluid of sugar beet leaves using cation exchange chromatography and reversed phase high performance liquid chromatography. based on amino acid sequence homology, including the presence of eight cysteines at conserved positions, the protein can be classified as a member of the plant family of non-specific lipid transfer proteins (nsltps). the protein is 47% identical to iwf1, an antifungal nsltp prev ... | 2000 | 10773337 |
| comparative analysis of the binding of antibodies prepared against the insect spodoptera exigua and against the mycopathogen nomuraea rileyi. | polyclonal antibodies were produced in mice against spodoptera exigua (beet armyworm) larval hemolymph and hemocytes and against cell wall surfaces of hyphal bodies and hyphae of the entomopathogenic hyphomycete nomuraea rileyi. in addition to exhibiting strong activity against their original antigenic substrates, all of the antibodies cross-react extensively with other substrates. the hemolymph antibody binds to hemocytes and vice versa, and both antibodies cross-react to the insect fat body ba ... | 2000 | 10772323 |
| design of a simulated moving bed unit for sucrose-betaine separations. | the results of a series of pulse experiments carried out in a fixed bed column packed with a strongly acid ion-exchange resin and with reference to the separation of an aqueous sucrose-betaine mixture are presented. these data were used to evaluate equilibrium and diffusion parameters for both key components in the frame of linear chromatography theory. the estimated values were used to design a simulated moving bed plant aimed to separate betaine from sucrose in beet molasses. | 2000 | 10749484 |
| [cloning of full-length cdna of the shallots virus x genome and infectivity of its transcripts in sugar beet protoplasts]. | 2000 | 10732355 | |
| vacuolar transport of the glutathione conjugate of trans-cinnamic acid. | red beet (beta vulgaris l.) tonoplast membrane vesicles and [14c]trans-cinnamic acid-glutatione were used to study the vacuolar transport of phynylpropanoid-glutathione conjugates which are formed in peroxidase-mediated reactions. it was determined that the uptake of [14c]trans-cinnamic acid-glutathione into the tonoplast membrane vesicles was mgatp dependent and was 10-fold faster than the uptake of non-conjugated [14c]trans-cinnamic acid. uptake of the conjugate in the presence of mgatp was no ... | 2000 | 10731020 |
| effect of type and quantity of concentrates on superovulation and embryo yield in beef heifers. | embryo yield and quality can be decreased following superovulation of cattle on high levels of concentrates. concentrate type can alter rumen fermentation patterns and so affect energy availability and thus embryo quality. the objectives of this experiment were to examine the effect of 2 levels and 2 types of concentrate on superovulatory response and embryo quality in beef heifers. beef heifers received grass silage as a basal diet and barley at 3 kg (n = 20) or ad-libitum (n = 19), or citrus/b ... | 1999 | 10729090 |
| [variability of rna in morphogenesis of sugar beet and sunflower leaves]. | we investigated the developmental gene expression patterns of beta vulgaris l. using dna: rna hybridization reactions. we found that 2,100 and 900 diversive poly(a)+mrna were synthesized while 430 and 4,000 diversive poly(a)+mrna were disappeared during greening in cells of cotyledons and during senescence in cells of yellowing leaves respectively. in the other periods of sugar beet vegetation differential poly(a)+mrna transcription failed to be revealed in the cells of green leaves within the l ... | 2015 | 10726306 |
| from sugar beet molasses to lyphan. integrated quality management from the raw material to the drug. | l-tryptophan is produced at the amino gmbh (frellstedt, frg) via biocatalytical condensation of the amino acid l-serine with indole. as a biocatalyst, tryptophan synthetase is used which is produced in high activities by a natural mutant escherichia coli strain. the enzyme mechanism and specificity and the individual process-parameters for the biotransformation procedure are explained as well as the purification process of educts and products. this includes a detailed description of the quality ... | 1999 | 10721087 |
| genetic analysis of the cell-to-cell movement of beet yellows closterovirus. | a beet yellows closterovirus (byv) variant expressing green fluorescent protein and leaves of byv local lesion host claytonia perfoliata were used to reveal genetic requirements for byv cell-to-cell movement in leaf epidermis and mesophyll. a series of mutations targeting genes that are not involved in amplification of the viral positive-strand rna was analyzed. the products of genes coding for a 6-kda hydrophobic protein (p6) and a 64-kda protein (p64), as well as for minor and major capsid pro ... | 2000 | 10683341 |
| effects of base ingredient in cooked molasses blocks on intake and digestion of prairie hay by beef steers. | twelve steers (332 kg) were used in three simultaneous 4 x 3 incomplete latin squares to evaluate effects of beet molasses (beet), cane molasses (cane), or concentrated separator by-product (csb) as base ingredients in cooked molasses blocks on intake and digestion of prairie hay and ruminal characteristics. all steers had ad libitum access to prairie hay (5.9% cp and 69.4% ndf; dm basis). the four experimental treatments included a control (no supplement) and three cooked molasses blocks, based ... | 2000 | 10682818 |
| iron deficiency decreases the fe(iii)-chelate reducing activity of leaf protoplasts. | the ferric-chelate reductase (fc-r) activity of mesophyll protoplasts isolated from fe-sufficient (control) and fe-deficient sugar beet (beta vulgaris l.) leaves has been characterized. measurements were made in an ionic environment similar to that in the apoplastic space of the sugar beet mesophyll cells. the fc-r activity of fe-sufficient and fe-deficient protoplasts was dependent on light. fe deficiency decreased markedly the fc-r activity per protoplast surface unit. the optimal ph for the a ... | 2000 | 10677427 |
| detection of beet yellows closterovirus methyltransferase-like and helicase-like proteins in vivo using monoclonal antibodies. | in the positive-stranded rna genome of beet yellows closterovirus (byv), the 5'-terminal orf 1a encodes a 295 kda polyprotein with the domains of papain-like cysteine proteinase, methyltransferase (mt) and helicase (hel), whereas orf 1b encodes an rna-dependent rna polymerase. eleven and five hybridoma cell lines secreting monoclonal antibodies (mabs) were derived from mice injected with the bacterially expressed fragments of the byv 1a product encompassing the mt and hel domains, respectively. ... | 2000 | 10675397 |
| variable number of tandem repeat loci in the mitochondrial genomes of beets. | we found four unrelated tandem repeat loci (tr1, tr2, tr3 and tr4) in the mitochondrial genomes of beets, with the tr1 locus embedded within a three-membered family of recombining repeat sequences (the rrn26-repeat). tr1 is composed of an array of 32-bp tandem repeats, the number of which varies from 2 to 13 among the seven beet genotypes examined. it is interesting to note that tr1 has 7-bp direct repeats flanking the array, which may be involved in the generation of the tandem repeat array. su ... | 2000 | 10672442 |
| the linkage disequilibrium between chloroplast dna and mitochondrial dna haplotypes in beta vulgaris ssp. maritima (l.): the usefulness of both genomes for population genetic studies. | the structure and evolution of the plant mitochondrial genome may allow recurrent appearance of the same mitochondrial variants in different populations. whether the same mitochondrial variant is distributed by migration or appears recurrently by mutation (creating homoplasy) in different populations is an important question with regard to the use of these markers for population genetic analyses. the genetic association observed between chloroplasts and mitochondria (i.e. two maternally inherite ... | 2000 | 10672158 |
| energetics of a strongly ph dependent rna tertiary structure in a frameshifting pseudoknot. | retroviruses employ -1 translational frameshifting to regulate the relative concentrations of structural and non-structural proteins critical to the viral life cycle. the 1.6 a crystal structure of the -1 frameshifting pseudoknot from beet western yellows virus reveals, in addition to watson-crick base-pairing, many loop-stem rna tertiary structural interactions and a bound na(+). investigation of the thermodynamics of unfolding of the beet western yellows virus pseudoknot reveals strongly ph-de ... | 2000 | 10669615 |
| improved detection of beet necrotic yellow vein virus in a das elisa by means of antibody single chain fragments (scfv) which were selected to protease-stable epitopes from phage display libraries. | the detection of beet necrotic yellow vein virus (bnyvv) in stored sugar beets by means of monoclonal antibodies or antibody single chain fragments (scfv) often poses problems, because the immunodominant c-terminal epitope of the viral coat protein is readily lost due to proteolysis. clones which produce scfv specific for protease-stable bnyvv epitopes were selected from two naive phage display libraries. fusion proteins of the scfv with a human igg kappa chain (expressed from the newly designed ... | 2000 | 10664416 |
| deletions in the kter-encoding domain, which is needed for polymyxa transmission, in manually transmitted isolates of beet necrotic yellow vein benyvirus. | one a and one b type isolate of beet necrotic yellow vein benyvirus which had been passaged for more than 15 years on manually inoculated chenopodium quinoa in our laboratory were found to have small deletions in the kter-encoding domain on rna 2 which is necessary for polymyxa transmission. there were no indications that these isolates and a third one, in which intact rna 2 was detected, contained mixtures of deleted and intact rnas or that populations of rnas 2 with different deletions includi ... | 2000 | 10664414 |
| pea embryonic tissues show common responses to the replication of a wide range of viruses. | the response of pea embryonic tissues to the replication of a range of different viruses was investigated using in situ hybridization to analyze changes in the expression of two host genes, heat shock protein 70 (hsp70) and lipoxygenase (lox1). excised pea embryos were infected using microprojectile bombardment with a nonseed transmissible strain of pea seed-borne mosaic potyvirus, or with pea early browning tobravirus (pebv), white clover mosaic potexvirus, or beet curly top geminivirus. collec ... | 2000 | 10662627 |
| a geographic information assessment of birth weight and crop production patterns around mother's residence. | a landscape approach using remote sensing (rs) and geographic information system (gis) technologies was developed to identify the proximity of maternal residence to agricultural areas, and the association between crop production patterns around mothers residences and low birth weight was evaluated. satellite data obtained for weld county, colorado, in 1991 and 1993 were digitally processed to generate crop location maps for this study. gis procedures were then used to determine crop types within ... | 2000 | 10662530 |
| beticolins, nonpeptidic, polycyclic molecules produced by the phytopathogenic fungus cercospora beticola, as a new family of ion channel-forming toxins. | beticolins are toxins produced by cercospora beticola, a phytopathogenic fungus responsible for the leaf spot disease of sugar beet. they form a family of 20 nonpeptidic compounds (named b0 to b19) that share the same polycyclic skeleton but differ by isomeric configuration (ortho- or para-) and by a variable residue r (bridging two carbons in one of the six cycles). it has been previously shown that b0 assembles itself into a multimeric structure and forms ion channels into planar lipid bilayer ... | 2000 | 10659710 |
| a comparison of grain, oil and beet pulp as energy sources for the exercised horse. | high-grain diets for the exercising horse were compared with diets which provided 15% of the total caloric intake from either vegetable oil or a highly fermentable fibre source (beet pulp). six thoroughbreds age 3 years were fed one of 3 diets or 5 weeks in a replicated 3 x 3 latin square. the control diet was 3.65 kg of sweet feed (sf), 0.9 kg of a protein/vitamin/mineral pellet and 5.45 kg of hay cubes. the fat diet replaced 1.15 kg of sf with 0.45 kg of soybean oil and the fibre diet replaced ... | 1999 | 10659304 |
| an alpha-l-arabinofuranosidase from penicillium purpurogenum: production, purification and properties. | penicillium purpurogenum secretes arabinofuranosidase to the growth medium. highest levels of enzyme (1.0 u ml(-1)) are obtained when l-arabitol is used as carbon source, while 0.85 and 0.7 u ml(-1) are produced with sugar beet pulp and oat spelts xylan, respectively. by means of a zymogram, three bands with arabinofuranosidase activity have been detected in the supernatant of a culture grown in oat spelts xylan. one of the enzymes was purified to homogeneity from this supernatant using gel filt ... | 2000 | 10656340 |
| efficient expression of foreign proteins in roots from tobravirus vectors. | viral vectors were constructed from infectious cdna clones of each of the three tobraviruses, tobacco rattle virus (trv), pea early-browning virus (pebv), and pepper ringspot virus (peprsv). rna2 of each of the three viruses was modified to carry an additional coat protein subgenomic promoter and was used to express green fluorescent protein (gfp) when inoculated to plants. the tobravirus expression vectors have a wide host range and were able to express gfp in, for example, nicotiana species, t ... | 2000 | 10648180 |
| dietary carbohydrates and fat influence radiographic bone mineral content of growing foals. | hydrolyzable carbohydrate intake in horse diets may become excessive when rapidly growing pastures are supplemented with grain-based concentrates. the substitution of fat and fiber for hydrolyzable carbohydrate in concentrates has been explored in exercising horses but not in young, growing horses. our objective was to compare bone development in foals that were fed pasture and concentrates rich in sugar and starch (corn, molasses) or fat and fiber (corn oil, beet pulp, soybean hulls, oat straw) ... | 1999 | 10641881 |
| effects of point mutations in the readthrough domain of the beet western yellows virus minor capsid protein on virus accumulation in planta and on transmission by aphids. | point mutations were introduced into or near five conserved sequence motifs of the readthrough domain of the beet western yellows virus minor capsid protein p74. the mutant virus was tested for its ability to accumulate efficiently in agroinfected plants and to be transmitted by its aphid vector, myzus persicae. the stability of the mutants in the agroinfected and aphid-infected plants was followed by sequence analysis of the progeny virus. only the mutation y201d was found to strongly inhibit v ... | 2000 | 10627524 |
| betaine in wine: answer to the french paradox? | in france, a diet high in saturated fat and cholesterol is associated with low coronary artery disease mortality and it may be that drinking wine is protective against ischemic heart disease. recent studies suggest that high plasma homocysteine concentrations are an independent risk factor for coronary, cerebral and peripheral arterial occlusive diseases. one of several routes for metabolism of homocysteine involves methylation using betaine as the methyl donor. betaine is often added to less ex ... | 1999 | 10616037 |
| hsp70 homolog functions in cell-to-cell movement of a plant virus. | plant closteroviruses encode a homolog of the hsp70 (heat shock protein, 70 kda) family of cellular proteins. to facilitate studies of the function of hsp70 homolog (hsp70h) in viral infection, the beet yellows closterovirus (byv) was modified to express green fluorescent protein. this tagged virus was competent in cell-to-cell movement, producing multicellular infection foci similar to those formed by the wild-type byv. inactivation of the hsp70h gene by replacement of the start codon or by del ... | 1999 | 10611288 |
| osmotic water permeability of isolated vacuoles | we measured the osmotic water permeability (p(os)) of vacuoles isolated from onion (allium cepa l.), rape (brassica napus l.), petunia (petunia hybrida hook.) and red beet (beta vulgaris l.). for all the vacuolar types investigated, p(os) values were in the range 200-1000 &mgr;m s(-1). the change in membrane surface area induced by an osmotic gradient was smaller than 2-6%. the vacuolar p(os) values for red beet and onion were reduced by 1 mm hgcl(2), to 14% and 30% of the control values, respec ... | 1999 | 10592035 |
| pcr-based cloning and segregation analysis of functional gene homologues in beta vulgaris. | to analyse genetic factors that potentially affect sugar quality and yield in beta vulgaris, we designed primers based on 18 homologous ests and conserved regions of 32 heterologous ests encoding gene products that act in the calvin cycle, the oxidative pentose phosphate cycle, photorespiration, synthesis, transport and degradation of sucrose, glycolysis, the citric acid cycle, nitrogen metabolism and osmoprotection. data on the amplification of 54 gene homologues from b. vulgaris are presented. ... | 1999 | 10589840 |
| specific mutations in a viral rna pseudoknot drastically change ribosomal frameshifting efficiency. | many viruses regulate protein synthesis by -1 ribosomal frameshifting using an rna pseudoknot. frameshifting is vital for viral reproduction. using the information gained from the recent high-resolution crystal structure of the beet western yellow virus pseudoknot, a systematic mutational analysis has been carried out in vitro and in vivo. we find that specific nucleotide tertiary interactions at the junction between the two stems of the pseudoknot are crucial. a triplex is found between stem 1 ... | 1999 | 10588689 |
| impact of gene flow from cultivated beet on genetic diversity of wild sea beet populations | gene flow and introgression from cultivated plants may have important consequences for the conservation of wild plant populations. cultivated beets (sugar beet, red beet and swiss chard: beta vulgaris ssp. vulgaris) are of particular concern because they are cross-compatible with the wild taxon, sea beet (b.vs. ssp. maritima). cultivated beet seed production areas are sometimes adjacent to sea beet populations; the numbers of flowering individuals in the former typically outnumber those in the p ... | 1999 | 10583835 |
| complete sequence of rna 1 and the presence of trna-like structures in all rnas of potato mop-top virus, genus pomovirus. | the complete nucleotide sequence (6043 nt) of rna 1 from potato mop-top virus (pmtv-sw), the type member of the genus pomovirus, was determined. the first (5'-terminal) open reading frame (orf 1) encodes a predicted protein of 148 kda. orf 2 extends through the opal stop codon of orf 1 producing a predicted readthrough protein of 206 kda which resembles the rna-dependent rna polymerases (rdrp) of other fungal-transmitted viruses. it includes a methyltransferase, a helicase and a gdd rdrp motif, ... | 1999 | 10573175 |
| role of the beet western yellows virus readthrough protein in virus movement in nicotiana clevelandii. | luteoviruses such as beet western yellows polerovirus (bwyv) are confined to and multiply within the phloem compartment of their hosts. the readthrough domain (rtd) of the minor bwyv capsid protein p74 is required for efficient virus accumulation in nicotiana clevelandii. experiments were carried out to determine if the low virus titres observed following agro-inoculation of whole plants with certain rtd mutants are due to a defect in virus multiplication in the nucleate cells of the phloem comp ... | 1999 | 10573174 |
| glucuronic acid directly linked to galacturonic acid in the rhamnogalacturonan backbone of beet pectins. | sugar-beet pulp was de-esterified and submitted to 72 h hydrolysis by 0.1 m hcl at 80 degrees c. oligomers containing a single glucuronic acid (glca) moiety in addition to n(>/= 2) repeats of the dimer -->4)-alpha-d-galpa-(1-->2)-alpha-l-rhap-(1--> were isolated from the hydrolysate by ion-exchange and gel-permeation. glycosyl linkage composition analysis and 1h nmr studies indicated that the glca was attached to o-3 of a galacturonic acid (gala) residue, as shown for the two pentamers beta-d-gl ... | 1999 | 10561599 |
| microbacterium kitamiense sp. nov., a new polysaccharide-producing bacterium isolated from the wastewater of a sugar-beet factory. | two strictly aerobic, heterotrophic and mesophilic new organisms, strains kitami a1 and kitami c2t, were isolated from the wastewater of a sugar-beet factory in kitami city, hokkaido, japan. in batch cultures, these organisms produced both insoluble and soluble exopolysaccharides (epss) utilizing sucrose as the sole carbon source. the g + c contents of the strains kitami c2t and kitami a1 were 69.2 mol%. both strains had anteiso-c15:0 acid, anteiso-c17:0 acid and iso-c16:0 as major components. t ... | 1999 | 10555312 |
| chemical and sensory properties of liquid beet sugar. | sensory analysis and headspace gas chromatography-flame ionization detection (gc-fid) analysis of liquid sugars (sucrose) designated for food-industrial use were performed in order to elucidate which compounds are responsible for perceived off-odors and off-flavors in liquid sugar. the liquid sugar samples investigated had various kinds of off-odors and off-flavors that might influence the quality of any food product in which they might be used, such as a jam or a soft drink. the sensory scores ... | 1999 | 10552814 |
| flupyrsulfuron soil dissipation and mobility in winter wheat crops. | residues of the sulfonylurea herbicide flupyrsulfuron were extracted from cropping soils with 0.1 m nahco(3). the soil extracts were cleaned up by partitioning and repeated thin-layer chromatography. flupyrsulfuron was transformed by diazomethane into n-(4, 6-dimethoxypyrimidine-2-yl)-n-(3-methoxycarbonyl-6-trifluoromet hylpyr idine-2-yl)methylamine (2), which was analyzed by gas-liquid chromatography with electron capture detection, and confirmation for several samples was made by gas chromatog ... | 1999 | 10552737 |
| plant production of volatile semiochemicals in response to insect-derived elicitors. | an increase in the release of volatile compounds by plants in response to insect feeding is triggered by interaction of elicitors in the oral secretions of insect herbivores with damaged plant tissues. this herbivore damage triggers de novo biosynthesis of volatile plant metabolites derived from several different biochemical pathways. natural enemies of herbivores use these volatile semiochemicals to locate their hosts. although some volatile compounds are released from storage in plants immedia ... | 1999 | 10549550 |
| asymmetric block of the plant vacuolar ca(2+)-permeable channel by organic cations. | in this work we have analysed the voltage-dependent block of the slow activating channel from red beet vacuoles by tris, quaternary ammonium ions and the natural polyamines putrescine, spermidine and spermine. all these organic cations when applied from the cytosolic side blocked the channel by binding apparently deep (zdelta values in the range of 0.65-1.35) within the pore. tetraethylammonium ion did not pass the selectivity filter, whereas the cations with a smaller cross-section and tris cou ... | 1999 | 10541793 |
| ecological basis for biocontrol of damping-off disease by pseudomonas fluorescens 54/96 | pseudomonas fluorescens 54/96, originally isolated from the rhizosphere of sugar beet, has been shown to be commercially effective in field trials for the suppression of a number of fungal diseases of seedlings. in vitro and microcosm-based assays revealed that both the timing and method of application of bacteria were important for effective control of pythium ultimum, the causative agent of damping-off disease. following transposon mutagenesis (tn5lac), mutants deficient for the suppression of ... | 1999 | 10540249 |
| influence of long-term feeding of limited amounts of phosphorus on dry matter intake, milk production, and body weight of dairy cows. | for almost two lactations, 24 high-yielding, multiparous dairy cows were fed a basal diet and concentrate mixtures with three different p concentrations. the basal diet consisted of grass (silage or artificially dried), corn silage, wet beet pulp, straw, and concentrates. the concentrate mixtures differed only in p content by varying the amount of monosodium phosphate. the number of cows and the amount of dietary p, expressed as a percentage of current recommendations in the netherlands were: 6 ... | 1999 | 10531601 |
| methyl jasmonate induces expression of a novel brassica juncea chitinase with two chitin-binding domains. | we have cloned a 1.3 kb brassica juncea cdna encoding bjchi1, a novel acidic chitinase with two chitin-binding domains that shows 62% identity to nicotiana tabacum chia1 chitinase. bjchi1 is structurally unlike chia1 that has one chitin-binding domain, but resembles chia5 chitinase uda1, the precursor of urtica dioica agglutinin: however there is only 36.9% identity between them. we propose that bjchi1 should be classified under a new class, chia7. the spacer and the hinge region of bjchi1 are p ... | 1999 | 10527425 |
| determination of endogenous formaldehyde in plants (fruits) bound to l-arginine and its relation to the folate cycle, photosynthesis and apoptosis. | a very powerful nucleophilic reagent, hydralazine(1-hydrazino-phtalazine) proved to be suitable for determination of the endogenous formaldehyde level in biological samples. it was found that in different plants (vegetables, fruits, especially in red beet, cauliflower, kohlrabi, grapes) is a large amount of releasable endogenous formaldehyde (0.5-1.0 mm) bound to l-arginine mainly in the form of n(g)-trihydroxymethyl-l-arginine (trihma). n(g)-hydroxymethyl-l-arginines (hma) were proved to transf ... | 1998 | 10526968 |
| chitinolytic activity of pseudomonas fluorescens isolates from barley and sugar beet rhizosphere. | twelve isolates of pseudomonas fluorescens, isolated from barley and sugar beet rhizosphere as antagonists towards the plant pathogenic microfungi rhizoctonia solani and pythium ultimum, showed chitinolytic activity in batch cultures when grown in media without exogenous chitin. enzyme tests in cultures demonstrated a complete array of chitinolytic enzymes. endochitinase and chitobiosidase activities seemed to be tightly coupled in the isolates, while there was no relationship to n-acetyl-glucos ... | 1999 | 10525178 |
| calcium-induced calcium release mediated by a voltage-activated cation channel in vacuolar vesicles from red beet. | little is known about the mechanisms underlying calcium-induced ca2+ release (cicr) in plants. the slow-activating vacuolar (sv) channel is both permeable to, and activated by ca2+, and is therefore a prime candidate for a role in cicr. cytosol-side-out vacuolar membrane vesicles loaded with 45ca2+ showed voltage- and ca(2+)-dependent ca2+ release, which was sensitive to the sv channel modulators dids, protein phosphatase 2b and calmodulin. significantly, voltage-dependent ca2+ release strongly ... | 1999 | 10518930 |
| alterations in organization and transcription of the mitochondrial genome of cytoplasmic male sterile sugar beet (beta vulgaris l.). | we have constructed a physical map of the mitochondrial dna of a cytoplasmic male sterile (cms) sugar beet line, tk81-ms, and compared it with that published for normal fertile sugar beet (cv. tk81-o) to clarify the differences between the cms and normal mitochondrial genomes. the tk81-ms genome is present as a single circular molecule of 481.8 kb, or as two molecules of 184.9 and 296.9 kb. the cms genome was found to be highly rearranged relative to the normal mitochondrial genome, with at leas ... | 1999 | 10517324 |
| identification of bacillus thuringiensis delta-endotoxin cry1c domain iii amino acid residues involved in insect specificity. | cry1c domain iii amino acid residues involved in specificity for beet armyworm (spodoptera exigua) were identified. for this purpose, intradomain iii hybrids between cry1e (nontoxic) and cry1e-cry1c hybrid g27 (toxic) were made. crossover points of these hybrids defined six sequence blocks containing between 1 and 19 of the amino acid differences between cry1e and g27. blocks b, c, d, and e of g27 were shown to be required for optimal activity against s. exigua. block e was also required for opt ... | 1999 | 10508062 |
| possible role of xanthobaccins produced by stenotrophomonas sp. strain sb-k88 in suppression of sugar beet damping-off disease. | three antifungal compounds, designated xanthobaccins a, b, and c, were isolated from the culture fluid of stenotrophomonas sp. strain sb-k88, a rhizobacterium of sugar beet that suppresses damping-off disease. production of xanthobaccin a in culture media was compared with the disease suppression activities of strain sb-k88 and less suppressive strains that were obtained by subculturing. strain sb-k88 was applied to sugar beet seeds, and production of xanthobaccin a in the rhizosphere of seedlin ... | 1999 | 10508056 |
| regulation of closterovirus gene expression examined by insertion of a self-processing reporter and by northern hybridization. | a reporter open reading frame (orf) coding for a fusion of bacterial beta-glucuronidase (gus) with a proteinase domain (pro) derived from tobacco etch potyvirus was utilized for tagging individual genes of beet yellows closterovirus (byv). insertion of this reporter orf between the first and second codons of the byv orfs encoding the hsp70 homolog (hsp70h), a major capsid protein (cp), and a 20-kda protein (p20) resulted in the expression of the processed gus-pro reporter from corresponding subg ... | 1999 | 10482546 |
| [content of nitrates and nitrites in market vegetables in wroclaw in the years 1996-1997]. | the aim of analytical investigation included in this paper was the determination the nitrate and nitrite contents in 8 common vegetables: cabbage, carrot, parsley, potato, garden beet, cucumber, lettuce and radish in relation to the acceptable levels of nitrates. the samples of vegetables were taken from detail shops in wrocław in the years 1996-1997. a total of 151 samples of vegetables were analyzed. the nitrate and nitrite level was determined collorimetricaly with sulfanilic acid after previ ... | 1999 | 10474289 |
| development and field performance of a broad-spectrum nonviable asporogenic recombinant strain of bacillus thuringiensis with greater potency and uv resistance. | the main problems with bacillus thuringiensis products for pest control are their often narrow activity spectrum, high sensitivity to uv degradation, and low cost effectiveness (high potency required). we constructed a sporulation-deficient sigk(-) b. thuringiensis strain that expressed a chimeric cry1c/ab gene, the product of which had high activity against various lepidopteran pests, including spodoptera littoralis (egyptian cotton leaf worm) and spodoptera exigua (lesser [beet] armyworm), whi ... | 1999 | 10473413 |
| role of the 14-3-3 proteins in the regulation of h+-atpase activity in the plasma membrane of suspension-cultured sugar beet cells under cold stress. | all higher plants possess highly specific binding sites for fusicoccin, a metabolite of the fungus fusicoccum amygdali del. these sites are harboured in the plasma membranes and formed by a 14-3-3 protein dimer associated with the c-terminal autoinhibitory domain of h+-atpase. we considered the fusicoccin binding to plasma membranes to be an indicator of complexation between the 14-3-3 dimer and h+-atpase, we assessed the effect of cold stress on the interaction of these proteins in suspension-c ... | 1999 | 10452522 |
| effects of structural modifications upon the accumulation in planta of replicons derived from beet necrotic yellow vein virus rna 3. | beet necrotic yellow vein virus (bnyvv) rna 3 from which all but the 3' and 5' 'core' replication origins (promoters) have been deleted replicates when coinoculated to chenopodium quinoa with viral rnas 1 and 2. the resulting 'replicon' can be used to express inserted heterologous sequences in planta. the effects of alterations of replicon structure on its efficiency of accumulation in planta were examined. inclusion of up to approximately 240 nucleotides of sequence from the region immediately ... | 1999 | 10446653 |
| antitumor and antiviral activity of colombian medicinal plant extracts. | extracts of nine species of plants traditionally used in colombia for the treatment of a variety of diseases were tested in vitro for their potential antitumor (cytotoxicity) and antiherpetic activity. mtt (tetrazolium blue) and neutral red colorimetric assays were used to evaluate the reduction of viability of cell cultures in presence and absence of the extracts. mtt was also used to evaluate the effects of the extracts on the lytic activity of herpes simplex virus type 2 (hsv-2). the 50% cyto ... | 1999 | 10446015 |
| processing, disulfide pattern, and biological activity of a sugar beet defensin, ax2, expressed in pichia pastoris. | ax2 is a 46-amino-acid cysteine-rich peptide isolated from sugar beet leaves infected with the fungus cercospora beticola (sacc.). ax2 strongly inhibits the growth of c. beticola and other filamentous fungi, but has little or no effect against bacteria. ax2 is produced in very low amounts in sugar beet leaves, and to study the protein in greater detail with respect to biological function and protein structural analysis, the methylotrophic yeast pichia pastoris was used for large-scale production ... | 1999 | 10425158 |
| traumatic l1-l2 dislocation without fracture in a 6-year-old girl. incomplete neurologic deficit and total recovery. | a report of a rare complete dislocation of the first lumbar vertebra without fracture in a 6-year-old girl. | 1999 | 10423795 |
| isoesterases related to cell differentiation in plant tissue culture. | normal, habituated and transformed in vitro tissue lines of sugar beet (beta vulgaris l.), horseradish (armoracia lapathifolia gilib.) and potato (solanum tuberosum l.) were studied with regard to isoesterase patterns. isoenzymes were separated in gradient gels (5-12%) of polyacrylamide and by isoelectric focussing in ph range 4-9. 1- and 2-naphtylacetate were used as substrates of broad spectrum which cover also esterases (arylesterases and carboxylesterases) reacting with organophosphorous com ... | 1999 | 10421498 |
| fractionation of sugar beet pulp into pectin, cellulose, and arabinose by arabinases combined with ultrafiltration. | incubation of beet pulp with two arabinases (alpha-l-arabinofuranosidase and endo-arabinase), used singularly or in combination at different units of activity per gram of beet pulp, caused the hydrolysis of arabinan, which produced a hydrolyzate consisting mainly of arabinose. pectin and a residue enriched with cellulose were subsequently separated from the incubation mixture. the best enzymatic hydrolysis results were obtained when 100 u/g of beet pulp of each enzyme worked synergistically with ... | 1999 | 10417217 |
| evidence for three groups of sequence variants of beet necrotic yellow vein virus rna 5. | about half of japanese isolates of beet necrotic yellow vein virus (bnyvv) were found to contain rna 5 molecules, which were also detected in virus isolates from china and france. sequence comparisons of rna 5 (nucleotides 327 to 1171) in 25 isolates showed that there are up to 8% sequence differences, and that rna 5 variants fall into three groups: group i contains most of the japanese and chinese isolates, group ii two japanese isolates, and group iii four french isolates. the group i isolates ... | 1999 | 10416372 |
| [chemical composition of 2 wild vegetable species compared with chard]. | the nutritional quality of two wild vegetable species, kochia scoparia (ks) and chenopodium album (cha) was evaluated and compared with beta vulgaris var. cicla(chard) (bv), in order to propose their domestication as alternative protein sources. chemical percentual analysis (aoac), gaseous chromatography fatty acid determination, and antinutrient searching (by chemical, enzymatic and immunological method) were performed. protein quality was determined by the following indexes: net protein utiliz ... | 1999 | 10412504 |
| subcellular localization of the hsp70-homolog encoded by beet yellows closterovirus. | closteroviridae is the only viral family coding for a homolog of hsp70 (hsp70h). polyclonal antiserum to recombinant beet yellows closterovirus (byv) hsp70h was generated and used for immunogold labeling of the leaf samples derived from the infected nicotiana benthamiana plants. ultrastructural analysis revealed the preferential accumulation of byv in phloem, although occasional infection of the leaf mesophyll cells was also observed. the strongest hsp70h-specific labeling was associated with vi ... | 1999 | 10405369 |
| scale-up of citric acid fermentation by redox potential control | to obtain high citric acid productivity in aspergillus niger fermentation on beet molasses substrate, a certain redox potential profile with two maxima (260 and 280 mv) and two minima (180 and 80 mv) must be maintained. the most effective regulation of redox potential is by regulation of aeration and agitation. it has been shown that control of redox potential by aeration and agitation is a most successful method for scale-up from 10-l laboratory scale to the 100- and 1000-l pilot-plant scale, e ... | 1999 | 10404235 |
| ingestion of sugar beet fiber enhances irradiation-induced aberrant crypt foci in the rat colon under an apoptosis-suppressed condition. | the induction of aberrant crypt foci (acf) by irradiation of gamma-rays (60co), and the effect of dietary sugar beet fiber (sbf) on irradiation-induced acf were examined. we found that abdominal irradiation of gamma-rays could induce acf in the rat colon. the irradiation was performed once a week at a dose rate of 2 or 3 gy per irradiation. irradiation-induced acf were observed in the colon at 10 weeks after the first irradiation at dose of 2 gy for six times or 3 gy for four times. dietary sbf ... | 1999 | 10357780 |
| amelioration by glucose-6-phosphate and nadp of potato glycoalkaloid inhibition in cell, enzyme and liposome assays. | lysis of human erythrocytes by 20 microm chaconine was reduced by 0.5 mm glucose-6-phosphate (g6p) and nadp. both compounds caused approximately 50% inhibition of haemolysis at 1 mm. glucose, glucose-1-phosphate, rhamnose, galactose and galactose-6-phosphate were ineffective; nad was effective, although not to the extent of nadp. of the tested sugars, only g6p reduced solanine-induced haemolysis. g6p also reduced the synergistic haemolytic action of solanine and chaconine in combination. g6p and ... | 1999 | 10349728 |
| effect of rumen protected methionine supplementation on early lactational responses of dairy cows fed a grass silage and cereals diet. | to investigate the milk production limiting potential of a diet based on grass silage (40%), hay (15%), dried sugar beet pulp (13%) and grain compound mixture (32%), 28 multiparous cows in early lactation were randomly assigned to two treatment groups: a control group and a group receiving supplementary rumen protected methionine (rpmet) treatment (12 g intestinally available methionine/cow/day, given 1-120 days postpartum; smartamine; rpan's technology). the diet was formulated to meet the requ ... | 1999 | 10344080 |
| soybean hulls as a dietary fiber source for dogs. | in exp. 1, soybean hull samples were obtained from nine sources across the united states and analyzed for nutrient content to determine their suitability for inclusion in dog diets. compositional data revealed variation in both the amount of total dietary fiber (tdf; 63.8 to 81.2%) in the soybean hulls and the ratio of insoluble:soluble fiber (5.0:1 to 15.4:1). crude protein content varied widely among sources, ranging from 9.2 to 18.7%. an in vivo trial (exp. 2) was conducted using a premium do ... | 1999 | 10328357 |
| feruloyl esterase from aspergillus sp.: purification, properties, and action on natural substrates | an enzyme active toward the methyl ester of ferulic acid was isolated from the fungus aspergillus sp. and purified to homogeneity using ion-exchange and hydrophobic chromatography. the molecular weight of the enzyme is 42 kd, and its pi is 3.7. the enzyme has a ph optimum in the range 4-6 and a temperature optimum in the range 40 to 60 degrees c. using a number of synthetic and natural substrates, the enzyme was identified as a feruloyl esterase. the feruloyl esterase did not hydrolyze wheat str ... | 1999 | 10231593 |
| characterization of six bacteriophages of serratia liquefaciens cp6 isolated from the sugar beet phytosphere | six phages (phicp6-1 to phicp6-6) that are commonly found in the phytosphere of sugar beet (beta vulgaris var. amethyst) were investigated, and their relative impacts on their host (serratia liquefaciens cp6) were compared. there were fundamental differences between the two most abundant predators of cp6 (phicp6-1 and phicp6-4). like phicp6-2 and phicp6-5, phicp6-1 belonged to the family siphoviridae, while phicp6-4 exhibited the morphology of the family podoviridae. the other phages were member ... | 1999 | 10223986 |
| short-chain fatty acids suppress cholesterol synthesis in rat liver and intestine. | we previously showed that plasma cholesterol levels decreased following ingestion of a short-chain fatty acid (scfa) mixture composed of sodium salts of acetic, propionic, and butyric acids simulating cecal fermentation products of sugar-beet fiber (sbf). in the present study, we investigated whether hepatic and small intestinal cholesterol synthesis is involved in the cholesterol-lowering effects of scfa and sbf. in vitro (expt. 1) and in vivo (expt. 2) cholesterol synthesis rates and the diurn ... | 1999 | 10222383 |
| evaluation of pectin binding of heavy metal ions in aqueous solutions. | evaluation of adsorption performance of several industrially manufactured pectins towards some toxic heavy metals was carried out. adsorption isotherms for divalent cations in simulant aqueous solutions were measured and corresponding distribution coefficients were calculated. the following selectivity sequences we found for pectins: pb2+ >> cu2+ > co2+ > ni2+ >> zn2+ > cd2+. it was shown that a beet pectin exhibits a high affinity for pb2+ and cu2+ ions, an apple pectin for co2+ ion and a citru ... | 1999 | 10204240 |
| a cross-polarization, magic-angle-spinning, 13c-nuclear-magnetic-resonance study of polysaccharides in sugar beet cell walls | solid-state nuclear magnetic resonance relaxation experiments were used to study the rigidity and spatial proximity of polymers in sugar beet (beta vulgaris) cell walls. proton t1rho decay and cross-polarization patterns were consistent with the presence of rigid, crystalline cellulose microfibrils with a diameter of approximately 3 nm, mobile pectic galacturonans, and highly mobile arabinans. a direct-polarization, magic-angle-spinning spectrum recorded under conditions adapted to mobile polyme ... | 1999 | 10198090 |
| the decisive step in betaxanthin biosynthesis is a spontaneous reaction1 | experiments were performed to confirm that the aldimine bond formation is a spontaneous reaction, because attempts to find an enzyme catalyzing the last decisive step in betaxanthin biosynthesis, the aldimine formation, failed. feeding different amino acids to betalain-forming hairy root cultures of yellow beet (beta vulgaris l. subsp. vulgaris "golden beet") showed that all amino acids (s- and r-forms) led to the corresponding betaxanthins. we observed neither an amino acid specificity nor a st ... | 1999 | 10198080 |
| binding of water, oil, and bile acids to dietary fibers of the cellan type. | dietary fibers (df) of the "cellan" type (consisting mainly or exclusively of undestroyed cells) were prepared as ethanol-dried materials from apple, cabbage, sugar-beet, soybean hulls, wheat bran, and suspension cultures of chenopodium album l. and investigated with respect to their interactions with water, water-oil dispersions, bile acids, and oil. water binding and retention capacities were found to be especially high in cellans obtained from thin-walled raw material. water damp sorption by ... | 2013 | 10194401 |