Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| resveratrol-mediated gamma interferon reduction prevents airway inflammation and airway hyperresponsiveness in respiratory syncytial virus-infected immunocompromised mice. | respiratory syncytial virus (rsv) is the most important cause of severe, lower respiratory tract infections in infants, and rsv infections have been associated with chronic wheezing and asthma during childhood. however, the mechanism of rsv-induced airway inflammation and airway hyperresponsiveness (ahr) is poorly understood. furthermore, there are presently neither effective vaccines nor drugs available for the prevention or treatment of rsv infections. in this study, we investigated the effect ... | 2011 | 21937650 |
| the effects of hourly differences in air pollution on the risk of myocardial infarction: case crossover analysis of the minap database. | to investigate associations between air pollution levels and myocardial infarction (mi) on short timescales, with data at an hourly temporal resolution. | 2011 | 21933824 |
| Label-free quantitative proteomics reveals regulation of interferon-induced protein with tetratricopeptide repeats 3 (IFIT3) and 5'-3'-exoribonuclease 2 (XRN2) during respiratory syncytial virus infection. | ABSTRACT: A large quantitative study was carried out to compare the proteome of respiratory syncytial virus (RSV) infected versus uninfected cells in order to determine novel pathways regulated during viral infection. RSV infected and mock-infected HEp2 cells were lysed and proteins separated by preparative isoelectric focussing using offgel fractionation. Following tryptic digestion, purified peptides were characterized using label-free quantitative expression profiling by nano-ultra performanc ... | 2011 | 21933386 |
| Requirements for Human Respiratory Syncytial Virus Glycoproteins in Assembly and Egress from Infected Cells. | Human respiratory syncytial virus (HRSV) is an enveloped RNA virus that assembles and buds from the plasma membrane of infected cells. The ribonucleoprotein complex (RNP) must associate with the viral matrix protein and glycoproteins to form newly infectious particles prior to budding. The viral proteins involved in HRSV assembly and egress are mostly unexplored. We investigated whether the glycoproteins of HRSV were involved in the late stages of viral replication by utilizing recombinant virus ... | 2011 | 21931576 |
| chest physiotherapy using passive expiratory techniques does not reduce bronchiolitis severity: a randomised controlled trial. | chest physiotherapy (cp) using passive expiratory manoeuvres is widely used in western europe for the treatment of bronchiolitis, despite lacking evidence for its efficacy. we undertook an open randomised trial to evaluate the effectiveness of cp in infants hospitalised for bronchiolitis by comparing the time to clinical stability, the daily improvement of a severity score and the occurrence of complications between patients with and without cp. children <1 year admitted for bronchiolitis in a t ... | 2011 | 21927808 |
| Phase 1 Study of the Safety and Immunogenicity of a Live, Attenuated Respiratory Syncytial Virus and Parainfluenza Virus Type 3 Vaccine in Seronegative Children. | BACKGROUND:: Respiratory syncytial virus (RSV) and parainfluenza virus type 3 (PIV3) are important causes of lower respiratory tract illness and hospitalization in young children. Currently, there is no licensed vaccine against RSV or PIV3. METHODS:: In this randomized, phase 1, double-blind, placebo-controlled, dose-escalating study, 49 healthy RSV/PIV3-seronegative children 6 to <24 months of age were randomized 2:1 to receive 3 doses (at 104, 105, or 106 median tissue culture infective dose [ ... | 2011 | 21926667 |
| clinical and laboratory features of pertussis in infants at the onset of a california epidemic. | we report clinical characteristics and outcome of infants <3 months of age hospitalized with pertussis compared with viral respiratory infection (respiratory syncytial virus and influenza). patients with pertussis more often were afebrile, had more visits before admission, and had longer hospital stays. household coughing contacts were common. | 2011 | 21925678 |
| Virological and clinical characterization of respiratory infections in children attending an emergency department during the first autumn-winter circulation of pandemic A (H1N1) 2009 influenza virus. | Clin Microbiol Infect ABSTRACT: To characterize respiratory virus infections during the first autumn-winter season of pandemic A (H1N1) 2009 influenza virus (A/H1N1/2009) circulation, a prospective study in children attending a paediatric emergency department at the Sapienza University hospital, Rome, was conducted from November 2009 to March 2010. By means of both nasal washings and pharyngeal swabs, enrolled children were checked for 14 respiratory viruses. The majority of acute respiratory in ... | 2011 | 21923780 |
| interaction of enterovirus infection and cow's milk-based formula nutrition in type 1 diabetes-associated autoimmunity. | background: enteral virus infections and early introduction of cow's milk (cm)-based formula are among the suggested triggers of type 1 diabetes (t1d)- associated autoimmunity, although studies on their role have remained contradictory. here, we aimed to analyse whether interactions between these factors might clarify the controversies. materials: the study population comprised 107 subjects developing positivity for at least two t1d-associated autoantibodies and 446 control subjects from the fin ... | 2011 | 21922634 |
| Effectiveness of palivizumab prophylaxis in infants and children in Florida. | PURPOSE: Palivizumab effectiveness data on respiratory syncytial virus (RSV) infections are limited to trial settings and vary considerably between selected high-risk populations. This study aimed to evaluate effectiveness in a community-based sample. METHODS: We conducted a cohort study of children with =3?months Florida Medicaid fee-for-service eligibility between 1998 and 2004 who also had matching birth certificates. Children entered the cohort at the beginning of the RSV season, after a min ... | 2011 | 21919115 |
| th17 cytokines are critical for respiratory syncytial virus-associated airway hyperreponsiveness through regulation by complement c3a and tachykinins. | respiratory syncytial virus (rsv) infection is associated with serious lung disease in infants and immunocompromised individuals and is linked to development of asthma. in mice, acute rsv infection causes airway hyperresponsiveness (ahr), inflammation, and mucus hypersecretion. infected cells induce complement activation, producing the anaphylatoxin c3a. in this paper, we show rsv-infected wild-type mice produce th17 cytokines, a response not previously associated with viral infections. mice def ... | 2011 | 21918196 |
| evaluation of protective efficacy of respiratory syncytial virus vaccine against a and b subgroup human isolates in korea. | human respiratory syncytial virus (hrsv) is a significant cause of upper and lower respiratory tract illness mainly in infants and young children worldwide. hrsv is divided into two subgroups, hrsv-a and hrsv-b, based on sequence variation within the g gene. despite its importance as a respiratory pathogen, there is currently no safe and effective vaccine for hrsv. in this study, we have detected and identified the hrsv by rt-pcr from nasopharyngeal aspirates of korean pediatric patients. intere ... | 2011 | 21915262 |
| [a guinea pig model of respiratory syncytial virus infection for cough and its neurogenic inflammatory mechanism]. | to establish a guinea pig model of acute and postinfectious cough caused by respiratory syncytial virus (rsv) infection and investigate the role of neurogenic inflammation in its pathogenesis. | 2011 | 21914323 |
| autophagy-mediated dendritic cell activation is essential for innate cytokine production and apc function with respiratory syncytial virus responses. | the regulation of innate immune responses during viral infection is a crucial step to promote antiviral reactions. recent studies have drawn attention to a strong relationship of pathogen-associated molecular pattern recognition with autophagy for activation of apc function. our initial observations indicated that autophagosomes formed in response to respiratory syncytial virus (rsv) infection of dendritic cells (dc). to further investigate whether rsv-induced dc activation and innate cytokine p ... | 2011 | 21911604 |
| local interleukin-10 production during respiratory syncytial virus bronchiolitis is associated with post-bronchiolitis wheeze. | respiratory syncytial virus (rsv) is the most common cause of bronchiolitis in infants. following rsv bronchiolitis, 50% of children develop post-bronchiolitis wheeze (pbw). animal studies have suggested that interleukin (il)-10 plays a critical role in the pathogenesis of rsv bronchiolitis and subsequent airway hyperresponsiveness. previously, we showed that ex vivo monocyte il-10 production is a predictor of pbw. additionally, heterozygosity of the single-nucleotide polymorphism (snp) rs180087 ... | 2011 | 21910858 |
| equal virulence of rhinovirus and respiratory syncytial virus in infants hospitalized for lower respiratory tract infection. | respiratory syncytial virus (rsv) and rhinovirus (rv) are predominant viruses associated with lower respiratory tract infection in infants. we compared the symptoms of lower respiratory tract infection caused by rsv and rv in hospitalized infants. rv showed the same symptoms as rsv, so on clinical grounds, no difference can be made between these pathogens. no relation between polymerase chain reaction cycle threshold value and length of hospital stay was found. | 2011 | 21909047 |
| Evidence for a causal relationship between respiratory syncytial virus infection and asthma. | Respiratory syncytial virus (RSV) infects all children early in life, is the most common cause of infant lower respiratory tract infections, and causes disease exacerbations in children with asthma. Episodes of lower respiratory tract infection in early life are associated with asthma development. Whether RSV infection early in life directly causes asthma or simply identifies infants who are genetically predisposed to develop subsequent wheezing is debatable. Recent studies suggest that these tw ... | 2011 | 21905783 |
| Prenatal prevention of respiratory syncytial virus bronchiolitis. | 2011 | 21905778 | |
| impact of respiratory syncytial virus: the nurse's perspective. | respiratory syncytial virus (rsv) is a highly contagious virus, and is the major cause of lower respiratory tract infections in infants and toddlers worldwide. rsv infection poses serious health risks to young children during the first 2 years of life. several infant populations have been classified as high risk, and additional risk factors are known to increase the likelihood of severe rsv infection. treatment for active rsv infection is limited to the symptoms of infection rather than the unde ... | 2011 | 21902285 |
| Infection with multiple viruses is not associated with increased disease severity in children with bronchiolitis. | BACKGROUND: The clinical relevance of parallel detection of multiple viruses by real-time polymerase chain reaction (RT-PCR) remains unclear. This study evaluated the association between the detection of multiple viruses by RT-PCR and disease severity in children with bronchiolitis. METHODS: Children less than 2 years of age with clinical symptoms of bronchiolitis were prospectively included during three winter seasons. Patients were categorized in three groups based on disease severity; mild (n ... | 2011 | 21901859 |
| Viral aetiology of influenza-like illness in Belgium during the influenza A(H1N1)2009 pandemic. | The purpose of this investigation was to determine the proportion of influenza-like illness (ILI) attributable to specific viruses during the influenza A(H1N1)2009 pandemic and to describe the demographic and clinical characteristics of ILI due to respiratory viruses in Belgium. Nasopharyngeal swabs were collected from ILI patients by general practitioners (GPs) and paediatricians (PediSurv) and analysed for viruses. Of 139 samples collected from children <5 years of age by PediSurv, 86 were pos ... | 2011 | 21901635 |
| respiratory syncytial virus--united states, july 2007-june 2011. | each year in the united states, an estimated 75,000-125,000 hospitalizations related to respiratory syncytial virus (rsv)occur among children aged <1 year, and rsv infection results in approximately 1.5 million outpatient visits among children aged <5 years. in the united states, rsv season begins in the fall, peaks in winter, and ends in the late winter and early spring. however, the exact timing and duration vary from year to year and by geographic region. to describe trends in rsv seasonality ... | 2011 | 21900874 |
| viral-bacterial interactions and risk of acute otitis media complicating upper respiratory tract infection. | acute otitis media (aom) is a common complication of upper respiratory tract infection whose pathogenesis involves both viruses and bacteria. we examined risks of acute otitis media associated with specific combinations of respiratory viruses and acute otitis media bacterial pathogens. data were from a prospective study of children ages 6 to 36 months and included viral and bacterial culture and quantitative pcr for respiratory syncytial virus (rsv), human bocavirus, and human metapneumovirus. r ... | 2011 | 21900518 |
| rela ser276 phosphorylation-coupled lys310 acetylation controls transcriptional elongation of inflammatory cytokines in respiratory syncytial virus infection. | respiratory syncytial virus (rsv) is a negative-sense single-stranded rna virus responsible for lower respiratory tract infections (lrtis) in humans. in experimental models of rsv lrti, the actions of the nuclear factor κb (nf-κb) transcription factor mediate inflammation and pathology. we have shown that rsv replication induces a mitogen-and-stress-related kinase 1 (msk-1) pathway that activates nf-κb rela transcriptional activity by a process involving serine phosphorylation at serine (ser) re ... | 2011 | 21900162 |
| effect of respiratory syncytial virus infection on plasmacytoid dendritic cell regulation of allergic airway inflammation. | respiratory syncytial virus (rsv) can infect myeloid dendritic cells (mdcs) and regulate their function in the development of allergy. it has been widely reported that plasmacytoid dcs (pdcs) play a critical role in antiviral innate immunity. in contrast, not much is known about the role of pdcs in the interaction between allergy and viral infection. the purpose of the present study was to investigate the effect of rsv infection on pdc function in the regulation of allergic airway inflammation i ... | 2012 | 21894025 |
| twenty-first century vaccines. | in the twentieth century, vaccination has been possibly the greatest revolution in health. together with hygiene and antibiotics, vaccination led to the elimination of many childhood infectious diseases and contributed to the increase in disability-free life expectancy that in western societies rose from 50 to 78-85 years (crimmins, e. m. & finch, c. e. 2006 proc. natl acad. sci. usa 103, 498-503; kirkwood, t. b. 2008 nat. med 10, 1177-1185). in the twenty-first century, vaccination will be expe ... | 2011 | 21893537 |
| Molecular epidemiology of the SH (small hydrophobic) gene of human respiratory syncytial virus (HRSV), over 2 consecutive years. | Human respiratory syncytial virus (HRSV) strains were isolated from nasopharyngeal aspirates collected from 965 children between 2004 and 2005, yielding 424 positive samples. We sequenced the small hydrophobic protein (SH) gene of 117 strains and compared them with other viruses identified worldwide. Phylogenetic analysis showed a low genetic variability among the isolates but allowed us to classify the viruses into different genotypes for both groups, HRSVA and HRSVB. It is also shown that the ... | 2011 | 21893117 |
| Central role of dendritic cells in shaping the adaptive immune response during respiratory syncytial virus infection. | Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract disease in young children. Premature infants, immunocompromised individuals and the elderly exhibit the highest risk for the development of severe RSV-induced disease. Murine studies demonstrate that CD8 T cells mediate RSV clearance from the lungs. Murine studies also indicate that the host immune response contributes to RSV-induced morbidity as T-cell depletion prevents the development of disease despite sustaine ... | 2011 | 21887154 |
| respiratory syncytial virus load, viral dynamics, and disease severity in previously healthy naturally infected children. | respiratory syncytial virus (rsv) disease severity was thought to be a result of host immunopathology but alternatively may be driven by high-level viral replication. the relationships between rsv load, viral clearance dynamics, and disease severity have not been carefully evaluated. | 2011 | 21881113 |
| Viruslike particle vaccine induces protection against respiratory syncytial virus infection in mice. | Respiratory syncytial virus (RSV) is the leading cause of bronchiolitis and viral death in infants. Despite decades of research with traditional or subunit vaccine approaches, there are no approved RSV vaccines. New approaches are therefore urgently needed to develop effective RSV vaccines. | 2011 | 21881112 |
| disease burden of the most commonly detected respiratory viruses in hospitalized patients calculated using the disability adjusted life year (daly) model. | the most common acute infections occur in the respiratory tract. recent discoveries of several novel viruses have markedly increased the repertoire of agents understood to cause presentations of acute respiratory disease. | 2011 | 21880543 |
| The first two nucleotides of the respiratory syncytial virus antigenome RNA replication product can be selected independently of the promoter terminus. | There is limited knowledge regarding how the RNA-dependent RNA polymerases of the nonsegmented negative-strand RNA viruses initiate genome replication. In a previous study of respiratory syncytial virus (RSV) RNA replication, we found evidence that the polymerase could select the 5'-ATP residue of the genome RNA independently of the 3' nucleotide of the template. To investigate if a similar mechanism is used during antigenome synthesis, a study of initiation from the RSV leader (Le) promoter was ... | 2011 | 21878549 |
| Decreased lung function after preschool wheezing rhinovirus illnesses in children at risk to develop asthma. | Preschool rhinovirus (RV) wheezing illnesses predict an increased risk of childhood asthma; however, it is not clear how specific viral illnesses in early life relate to lung function later on in childhood. | 2011 | 21878241 |
| The respiratory syncytial virus transcription antiterminator M(2-1) is a highly stable, zinc binding tetramer with strong pH-dependent dissociation and a monomeric unfolding intermediate. | The human respiratory syncytial virus M(2-1) transcription antiterminator is an essential elongation factor required by the RNA polymerase for effective transcription beyond the first two nonstructural genes. Its exclusive presence in pneumovirus among all paramyxovirus suggests a unique function within this small genus. With the aim of understanding its biochemical properties, we investigated this a-helical tetramer by making use of a biophysical approach. We found that the tetramer hydrodynami ... | 2011 | 21877705 |
| Wheezing lower respiratory disease and vaccination of premature infants. | Premature infants are at increased risk of wheezing in association with respiratory syncytial virus (RSV) and rhinovirus infections. We assess possible associations between wheezing and routine vaccinations of premature infants. | 2011 | 21875634 |
| [pilot study: serological research of bovine respiratory syncytial virus and mannheimia haemolytica in young cattle at dairy farms in the netherlands]. | 2011 | 21870575 | |
| flavone c-glycosides from the leaves of lophatherum gracile and their in vitro antiviral activity. | four new flavone c-glycosides, luteolin 6- c- α-l-arabinopyranosyl-7- o- β-d-glucopyranoside ( 1), apigenin 6- c- β-d-galactopyranosiduronic acid (1 → 2)- α-l-arabinopyranoside ( 2), luteolin 6- c- β-d-galactopyranosiduronic acid (1 → 2)- α-l-arabinopyranoside ( 3), and luteolin 6- c- β-d-glucopyranosiduronic acid (1 → 2)- α-l-arabinofuranoside ( 4), along with three known ones, were isolated from the leaves of lophatherum gracile. the structures of the new compounds were elucidated by extensi ... | 2011 | 21870321 |
| seasonal variations of 15 respiratory agents illustrated by the application of a multiplex polymerase chain reaction assay. | abstract background: nucleic acid amplification tests are increasingly being used to diagnose viral and bacterial respiratory tract infections. the high sensitivity of these tests affects our understanding of the epidemiology of respiratory tract infections. we have assessed the detection rate of a multiplex real-time polymerase chain reaction (pcr) test, with emphasis on epidemiology and seasonal distribution of the most common respiratory tract infections. methods: seven thousand eight hundr ... | 2012 | 21867470 |
| respiratory syncytial virus infection and the tight junctions of nasal epithelial cells. | respiratory syncytial virus (rsv) primarily infects upper respiratory tract cells, mainly nasal epithelial cells. the tight junctions of nasal epithelial cells are thought to perform important innate immune function against foreign materials including respiratory viruses. we investigated in vitro the relationship of rsv infection and the tight junctions of primary nasal epithelial cells which had been transfected with human telomerase reverse transcriptase (htert) to prolong cell life. nasal epi ... | 2011 | 21865717 |
| a study of the genetic variability of human respiratory syncytial virus (hrsv) in cambodia reveals the existence of a new hrsv group b genotype. | human respiratory syncytial virus (hrsv) is the leading cause of hospitalization of children aged <5 years due to respiratory illness in industrialized countries, and pneumonia is the leading cause of mortality among children aged <5 years worldwide. although hrsv was first identified in 1956, a preventative vaccine has yet to be developed. here we report the results of the first study to investigate the circulation and genetic diversity of hrsv in cambodia among an all-ages population over 5 co ... | 2011 | 21865418 |
| Primary airway epithelial cultures from children are highly permissive to respiratory syncytial virus infection. | Background Respiratory syncytial virus (RSV) infection of airway epithelial cells (AECs) is an important initial event in RSV bronchiolitis. AEC immunological responses are thought to be critical in driving the subsequent inflammation in the airway. This study examined viral replication, cytotoxicity and cytokine production in cultures of primary AECs from children compared with responses to RSV infection in an immortalised epithelial cell line and to those from infants with RSV bronchiolitis. ... | 2012 | 21865207 |
| bovine respiratory syncytial virus iscoms-immunity, protection and safety in young conventional calves. | bovine respiratory syncytial virus (brsv) is a major cause of bronchiolitis and pneumonia in cattle and causes yearly outbreaks with high morbidity in europe. commercial vaccines against this virus needs improvement of efficacy, especially in calves with brsv-specific maternally derived antibodies (mda). we previously reported that an experimental brsv-iscom vaccine, but not a commercial vaccine, induced strong clinical and virological protection in calves with mda, immunized at 7-15 weeks of ag ... | 2011 | 21864616 |
| rna viruses in young nepalese children hospitalized with severe pneumonia. | pneumonia is among the leading causes of illness and death in children <5 years of age worldwide. there is little information on the viral etiology of severe pneumonia in low-income countries, where the disease burden is particularly high. | 2011 | 21860338 |
| progress in the development of human parainfluenza virus vaccines. | in children under 5 years of age, human parainfluenza viruses (hpivs) as a group are the second most common etiology of acute respiratory illness leading to hospitalization, surpassed only by respiratory syncytial virus but ahead of influenza viruses. using reverse genetics systems for hpiv serotypes 1, 2 and 3 (hpiv1, 2 and 3), several live-attenuated hpivs have been generated and evaluated as intranasal vaccines in adults and in children. two vaccines against hpiv3 were found to be well tolera ... | 2011 | 21859271 |
| progress in respiratory virus vaccine development. | viral respiratory infections cause significant morbidity and mortality in infants and young children as well as in at-risk adults and the elderly. although many viral pathogens are capable of causing respiratory disease, vaccine development has to focus on a limited number of pathogens, such as those that commonly cause serious lower respiratory illness (lri). whereas influenza virus vaccines have been available for some time (see the review by clark and lynch in this issue), vaccines against ot ... | 2011 | 21858754 |
| Respiratory viral infections in hematopoietic stem cell and solid organ transplant recipients. | Respiratory viral infections (RVIs) are common causes of mild illness in immunocompetent children and adults with rare occurrences of significant morbidity or mortality. Complications are more common in the very young, very old, and those with underlying lung diseases. However, RVIs are increasingly recognized as a cause of morbidity and mortality in recipients of hematopoietic stem cell transplants (HSCT) and solid organ transplants (SOTs). Diagnostic techniques for respiratory syncytial virus ... | 2011 | 21858751 |
| human metapneumovirus. | human metapneumovirus (hmpv) is a common pathogen that can cause both upper and lower respiratory tract infections, particularly in children, elderly adults, and immunocompromised hosts. since its initial identification in 2001, hmpv has been isolated from individuals with acute respiratory tract infections (artis) in virtually every continent. serological studies indicate that it has caused human infection since 1958 or earlier. the epidemiology and clinical manifestations of hmpv are similar t ... | 2011 | 21858749 |
| respiratory syncytial virus infection in adults. | respiratory syncytial virus (rsv), an enveloped rna virus in the paramyxovirus family, is the most important cause of lower respiratory tract infection in infants and young children, accounting for ~100,000 pediatric hospitalizations and 250 deaths annually in the united states. despite primarily being recognized as a pediatric pathogen, rsv reinfection causes substantial disease in all adult populations, including healthy young persons, old and frail individuals, those with chronic obstructive ... | 2011 | 21858747 |
| Non-invasive sample collection for respiratory virus testing by multiplex PCR. | Identifying respiratory pathogens within populations is difficult because invasive sample collection, such as with nasopharyngeal aspirate (NPA), is generally required. PCR technology could allow for non-invasive sampling methods. | 2011 | 21855405 |
| identification of potential human respiratory syncytial virus and metapneumovirus t cell epitopes using computational prediction and mhc binding assays. | human respiratory syncytial virus (rsv) and human metapneumovirus (mpv) are two of the most common causes of serious viral lower respiratory tract illness in humans. cd8+ t cells have been shown to be important in animal models and human clinical studies for the clearance of viral infection, and they may contribute in part to protection against severe disease during reinfections. precise enumeration and accurate phenotyping of rsv- or mpv-specific cd8+ t cells in humans is currently limited by t ... | 2011 | 21854782 |
| RSV infection modulates IL-15 production and MICA levels in respiratory epithelial cells. | The cytokine interleukin-15 (IL-15), major histocompatibility complex (MHC) class I molecules and MHC class I chain-related proteins (MIC) A and B (MICA/B) are involved in cellular immune responses to virus infections but their role in respiratory syncytial virus (RSV) infection has not been studied. We aimed to determine how RSV infection modulates IL-15 production, MHC class I and MICA expression in respiratory epithelial cells, the molecular pathways implicated in virus-induced IL-15 producti ... | 2011 | 21852331 |
| first influenza season after the 2009 pandemic influenza: characteristics of intensive care unit admissions in adults and children in vall d'hebron hospital. | clin microbiol infect abstract: to assess potential differences in epidemiology and management of patients admitted with influenza infection in the intensive care unit (icu) during the first post-pandemic influenza period. observational prospective study comparing september 2009-january 2010 with september 2010-january 2011. variables captured: demographics, co-morbidities, physiological parameters, outcomes and management. analysis was performed using spss v. 13.0; significance was set at p 0.5 ... | 2011 | 21851487 |
| Krüppel-like factor 6 regulates transforming growth factor-ß gene expression during human respiratory syncytial virus infection. | Human respiratory syncytial virus (RSV) infection is associated with airway remodeling and subsequent asthma development. Transforming growth factor-beta (TGF) plays a crucial role in asthma development. The mechanism regulating TGF gene expression during RSV infection is not known. Kruppel-like factor family of transcription factors are critical regulators of cellular/tissue homeostasis. Previous studies have shown that Kruppel-like factor 6 (KLF6) could function as a trans-activator of TGF gen ... | 2011 | 21849067 |
| Respiratory syncytial virus and the premature infant parent. | 2011 | 21846631 | |
| multiple cd4+ t cell subsets produce immunomodulatory il-10 during respiratory syncytial virus infection. | the host immune response is believed to contribute to the severity of pulmonary disease induced by acute respiratory syncytial virus (rsv) infection. because rsv-induced pulmonary disease is associated with immunopathology, we evaluated the role of il-10 in modulating the rsv-specific immune response. we found that il-10 protein levels in the lung were increased following acute rsv infection, with maximum production corresponding to the peak of the virus-specific t cell response. the majority of ... | 2011 | 21844390 |
| viral co-detection in infants hospitalized with respiratory disease: is it important to detect? | 2011 | 21842109 | |
| identification of nucleolin as a cellular receptor for human respiratory syncytial virus. | human respiratory syncytial virus (rsv) causes a large burden of disease worldwide. there is no effective vaccine or therapy, and the use of passive immunoprophylaxis with rsv-specific antibodies is limited to high-risk patients. the cellular receptor (or receptors) required for viral entry and replication has yet to be described; its identification will improve understanding of the pathogenesis of infection and provide a target for the development of novel antiviral interventions. here we show ... | 2011 | 21841784 |
| respiratory viruses involved in influenza-like illness in a greek pediatric population during the winter period of the years 2005-2008. | viruses are the major cause of pediatric respiratory tract infection and yet many suspected cases of illness remain uncharacterized. this study aimed to determine the distribution of several respiratory viruses in children diagnosed as having influenza-like illness, over the winter period of 2005-2008. molecular assays including conventional and real time pcr protocols, were employed to screen respiratory specimens, collected by clinicians of the influenza sentinel system and of outpatient pedia ... | 2011 | 21837803 |
| confirmation of an association between single nucleotide polymorphisms in the vdr gene with respiratory syncytial virus related disease in south african children. | respiratory syncytial virus is a leading cause of lower respiratory tract infection in infants. disease severity has been linked to host immune responses and polymorphisms in genes associated with innate immunity. a large-scale genetics study of single nucleotide polymorphisms (snps) in children in the netherlands identified snps in the vitamin d receptor (vdr) and jun genes which have a strong association with an increased risk of developing bronchiolitis following the first respiratory syncyti ... | 2011 | 21837802 |
| antibody response and avidity of respiratory syncytial virus-specific total igg, igg1, and igg3 in young children. | respiratory syncytial virus (rsv) is a major cause of acute respiratory disease in infants and young children. considering that several aspects of the humoral immune response to rsv infection remain unclear, this study aimed to investigate the occurrence, levels, and avidity of total igg, igg1, and igg3 antibodies against rsv in serum samples from children ôëñ5 years old. in addition, a possible association between antibody avidity and severity of illness was examined. the occurrence and levels ... | 2011 | 21837801 |
| human metapneumovirus in patients with respiratory tract infection in kuwait. | human metapneumovirus (hmpv) has been recognized as an important cause of respiratory tract infections in all age groups and in all geographical area. the role of hmpv in causing respiratory tract infections in kuwait was not yet investigated. the aim of this study was to determine the prevalence of hmpv infection in kuwait among patients with respiratory tract infection with respect to other respiratory viruses. during january-december 2009, 460 respiratory samples from 388 patients with respir ... | 2011 | 21837799 |
| tak1 regulates nf-+üb and ap-1 activation in airway epithelial cells following rsv infection. | respiratory syncytial virus (rsv) is the most common cause of epidemic respiratory diseases in infants and young children. rsv infection of airway epithelial cells induces the expression of immune/inflammatory genes through the activation of a subset of transcription factors, including nuclear factor-+¦b (nf-+¦b) and ap-1. in this study, we have investigated the signaling pathway leading to activation of these two transcription factors in response to rsv infection. our results show that ikk+¦ pl ... | 2011 | 21835421 |
| prevalence of respiratory pathogens in diseased, non-vaccinated, routinely medicated veal calves. | the prevalence of respiratory pathogens in diseased veal calves was determined in 24 respiratory disease outbreaks in 15 herds in belgium. bacteria were cultured from nasopharyngeal swabs and seroconversion against viruses and mycoplasma bovis was determined on paired sera. at the individual calf level, mycoplasma species, mannheimia haemolytica and pasteurella multocida, were isolated from 70.5 per cent, 21.5 per cent and 26.0 per cent of swabs, respectively. at the herd level, the presence of ... | 2011 | 21831999 |
| a review of palivizumab and emerging therapies for respiratory syncytial virus. | introduction: respiratory syncytial virus (rsv) is an important pathogen in children and adults; however, current treatment options are primarily supportive. palivizumab, the only approved specific monoclonal antibody for rsv is used prophylactically to reduce morbidity in a select population of high-risk children. areas covered: the development and current use of palivizumab; the potential role of palivizumab as preventive therapy in patients with cystic fibrosis, asthma and compromised immune ... | 2011 | 21831008 |
| [acute viral respiratory infection in children under 5 years: epidemiological study in two centers in buenos aires, argentina]. | introduction. acute respiratory infections (ari) are a major cause of hospitalization particularly at the extreme ages of life. objectives. to determine the viral diagnosis in children < 5 years old with ari, seasonality, clinical and epidemiological characteristics. population and methods. a cross-sectional, descriptive and multicenter study was performed. respiratory samples from patients with ari hospitalized or outpatients with < 5 days of respiratory symptoms from june 2008 to may 2009 were ... | 2011 | 21829870 |
| circulating strains of human respiratory syncytial virus in central and south america. | human respiratory syncytial virus (hrsv) is a major cause of viral lower respiratory tract infections among infants and young children. hrsv strains vary genetically and antigenically and have been classified into two broad subgroups, a and b (hrsv-a and hrsv-b, respectively). to date, little is known about the circulating strains of hrsv in latin america. we have evaluated the genetic diversity of 96 hrsv strains by sequencing a variable region of the g protein gene of isolates collected from 2 ... | 2011 | 21829605 |
| respiratory syncytial virus prevention in children with congenital heart disease: who and how? | respiratory syncytial virus (rsv) is a major cause of respiratory infection in children. most of the pediatric population have rsv infection before the age of 2, and recurrent infections are common even within one season. chronic lung disease, prematurity, along with congenital heart disease (chd) are major risk factors in severe lower respiratory infection. in hemo-dynamically significant chd patients with rsv infection, hospitalization is usually needed and the possibility of treatment in inte ... | 2011 | 21829410 |
| respiratory syncytial virus infection in children with congenital heart disease: global data and interim results of korean rsv-chd survey. | respiratory syncytial virus (rsv) is a main cause of hospitalization for bronchiolitis and pneumonia in infants worldwide. children with hemodynamically significant congenital heart disease (hs-chd), as well as premature infants are at high risk for severe rsv diseases. mortality rates for chd patients hospitalized with rsv have been reported as about 24 times higher compared with those without rsv infection. recently with advances in intensive care, mortality rates in chd patients combined with ... | 2011 | 21829409 |
| autocrine regulation of pulmonary inflammation by effector t-cell derived il-10 during infection with respiratory syncytial virus. | respiratory syncytial virus (rsv) infection is the leading viral cause of severe lower respiratory tract illness in young infants. clinical studies have documented that certain polymorphisms in the gene encoding the regulatory cytokine il-10 are associated with the development of severe bronchiolitis in rsv infected infants. here, we examined the role of il-10 in a murine model of primary rsv infection and found that high levels of il-10 are produced in the respiratory tract by anti-viral effect ... | 2011 | 21829368 |
| decreased innate immune cytokine responses correlate with disease severity in children with respiratory syncytial virus and human rhinovirus bronchiolitis. | the immunopathogenesis of respiratory syncytial virus (rsv) and human rhinovirus lower respiratory tract infections in children remains to be defined. we measured nasal wash concentrations of 29 cytokines in infants with rsv or human rhinovirus lower respiratory tract infections. concentrations of interferon-+¦ in rsv and innate immunity cytokines in both infections inversely correlated with disease severity. | 2011 | 21829141 |
| RSV 2010: Recent advances in research on respiratory syncytial virus and other pneumoviruses. | Respiratory syncytial virus (RSV) and human metapneumovirus (HMPV) are important causes of acute respiratory tract disease in infants, immunocompromised patients and the elderly. The Seventh International RSV symposium was held in Rotterdam, the Netherlands, from December 2-5, 2010. This symposium is the flagship event for leading investigators engaged in RSV and HMPV research around the world. The objective of the symposium was to provide a forum to review recent advances in research on RSV, HM ... | 2011 | 21827813 |
| generation of recombinant metapneumovirus nucleocapsid protein as nucleocapsid-like particles and development of virus-specific monoclonal antibodies. | human metapneumovirus (hmpv) is a member of the pneumovirinea subfamily within the paramyxoviridea family. since its discovery in 2001, hmpv has been isolated in several continents, which suggests its prevalence worldwide. hmpv resembles human respiratory syncytial virus with regard to disease symptoms and its ability to infect and cause disease in young infants as well as individuals of all ages. the aim of the current study was to construct an efficient high-level yeast expression system for t ... | 2011 | 21827798 |
| respiratory syncytial virus infection is associated with an altered innate immunity and a heightened pro-inflammatory response in the lungs of preterm lambs. | abstract: | 2011 | 21827668 |
| respiratory syncytial virus-associated hospitalizations among infants and young children in the united states, 1997-2006. | background:: respiratory syncytial virus (rsv) is the most common cause of lower respiratory tract disease among young children in the united states. rsv-associated hospitalization increased among children in the united states during 1980 through 1996. in this study, we updated national estimates of rsv hospitalization rates among us children through 2006. methods:: we conducted a retrospective analysis of hospital discharges for lower respiratory tract illness (lrti) in children <5 years old fr ... | 2011 | 21817948 |
| type-iii interferon, not type-i, is the predominant interferon induced by respiratory viruses in nasal epithelial cells. | as an innate immune response against diverse viral infections, a host induces two types of interferon (ifn), type-i (ifn-+¦/+¦) and type-iii (ifn-++). we investigated ifn inductions by respiratory viruses, including respiratory syncytial virus (rsv), measles virus and mumps virus in human nasal epithelial cells (necs). ifn-++, but not ifn-+¦/+¦, was induced by respiratory virus infection in primary necs and immortalized necs through transfection with the human telomerase reverse transcriptase ge ... | 2011 | 21816185 |
| il10 family member genes il19 and il20 are associated with recurrent wheeze after respiratory syncytial virus bronchiolitis. | mechanisms underlying the increased risk of recurrent wheeze following respiratory syncytial virus lower respiratory tract infection (rsv lrti) are unclear. specifically, information about genetic determinants of recurrent wheeze following rsv lrti is limited. we performed a candidate gene association study to identify genetic determinants of recurrent wheeze following rsv lrti. we investigated 346 single nucleotide polymorphisms (snps) in 220 candidate genes in 166 dutch infants hospitalized fo ... | 2011 | 21814157 |
| household tobacco smoke and admission weight predict severe bronchiolitis in infants independent of deprivation: prospective cohort study. | to examine demographic, environmental and clinical factors associated with severe bronchiolitis in infants admitted to hospital and quantify the independent effects of these factors. | 2011 | 21811609 |
| liu-he-tang inhibited plaque formation by human respiratory syncytial virus infection in cell lines of the human respiratory tract. | ethnopharmacological relevance: liu-he-tang (lht) has been used to treat adult respiratory tract infection with productive cough and fever for a thousand years in ancient china. adults with respiratory tract infection of human respiratory syncytial virus (hrsv) can have symptoms similar to those managed by lht. therefore, lht is supposed to be beneficial for adult hrsv infection. however, lht does not have any antiviral activity to support its use against hrsv infection. aim of the study: hrsv i ... | 2011 | 21810459 |
| nutrition economics: an innovative approach to informed public health management. | the role of nutrition to optimize the use of scarce resources through its linkage with health and welfare should be considered of interest by healthcare decision makers. a favorable impact of food on non-communicable disorders and general health status will improve healthcare expenditure and quality of life.in health economics, an analysis of the costs and effects of a healthcare technology by means of a cost-effectiveness analysis has become an established tool. projections about the effectiven ... | 2011 | 21810421 |
| innate immunity proteins and a new truncated form of splunc1 in nasopharyngeal aspirates from infants with respiratory syncytial virus infection. | purpose: respiratory syncytial virus (rsv) is the most common cause of severe respiratory tract infection in infants. the aim was to identify host defence components in nasopharyngeal aspirate (npa) from infants with rsv infection and to study the expression of the novel 25 kda innate immunity protein splunc1. experimental design: npas from infants were analyzed with 2-de and ms in a pilot study. the levels of splunc1 were analyzed with immunoblotting in 47 npas, admitted for rsv diagnosis. resu ... | 2011 | 21805676 |
| respiratory syncytial virus therapy and prophylaxis: have we finally turned the corner? | 2011 | 21804160 | |
| absence of vaccine-enhanced rsv disease and changes in pulmonary dendritic cells with adenovirus-based rsv vaccine. | abstract: the development of a vaccine against respiratory syncytial virus (rsv) has been hampered by the risk for vaccine-enhanced rsv pulmonary disease induced by immunization with formalin-inactivated rsv (firsv). this study focuses on the evaluation of vaccine-enhanced pulmonary disease following immunization with adf.rgd, an integrin-targeted adenovirus vector that expresses the rsv f protein and includes an rgd (arg-gly-asp) motif. immunization of balb/c mice with adf.rgd, resulted in anti ... | 2011 | 21801372 |
| synthesis, antiviral and cytotoxic investigation of 2-phenyl-3-substituted quinazolin-4(3h)-ones. | a series of 3(benzylideneamino)-2-phenyl quinazoline-4(3h)-ones was synthesized by reaction of 3-amino-2-phenyl-3h-quinazoline-4-one with various carbonyl compounds. | 2011 | 21796872 |
| characterization of the interaction between human respiratory syncytial virus and the cell cycle in continuous cell culture and primary human airway epithelial cells. | viruses can create conditions inside cells more favorable for replication and progeny virus production. one way of doing this is through manipulation of the cell cycle, a process that describes the ordered growth and division of cells. analysis of model cell lines such as a549 cells and primary airway epithelial cells infected with human respiratory syncytial virus (hrsv) has shown alteration of the cell cycle during infection, although the signaling events were not clearly understood. in this s ... | 2011 | 21795354 |
| mhc-dependent ctl repertoire and functional avidity contribute to strain-specific disease susceptibility after murine rsv infection. | susceptibility to respiratory syncytial virus (rsv) infection in mice is genetically determined. while rsv causes little pathology in c57bl/6 mice, pulmonary inflammation and weight loss occur in balb/c mice. using mhc congenic mice, we observed that the h-2(d) allele can partially transfer disease susceptibility to c57bl/6 mice. this was not explained by altered viral elimination or differences in the magnitude of the overall virus-specific ctl response. however, h-2(d) mice showed a more focus ... | 2011 | 21795345 |
| multiple functional domains and complexes of the two nonstructural proteins of human respiratory syncytial virus contribute to interferon suppression and cellular location. | human respiratory syncytial virus (rsv), a major cause of severe respiratory diseases, efficiently suppresses cellular innate immunity, represented by type i interferon (ifn), using its two unique nonstructural proteins, ns1 and ns2. in searching for their mechanism, ns1 was previously shown to decrease levels of traf3 and ikke, whereas ns2 interacted with rig-i and decreased traf3 and stat2. here, we report on the interaction, cellular localization and functional domains of these two proteins. ... | 2011 | 21795342 |
| whole genome characterization of non-tissue culture adapted hrsv strains in severely infected children. | abstract: background: human respiratory syncytial virus (hrsv) is the most important virus causing lower respiratory infection in young children. the complete genetic characterization of rsv clinical strains is a prerequisite for understanding hrsv infection in the clinical context. current information about the genetic structure of the hrsv genome has largely been obtained using tissue culture adapted viruses. during tissue culture adaptation genetic changes can be introduced into the virus gen ... | 2011 | 21794174 |
| comparison of risk factors between preterm and term infants hospitalized for severe respiratory syncytial virus in the russian federation. | respiratory syncytial virus (rsv) is a leading cause of lower respiratory tract infection in infants. preterm birth, in addition to several demographic and environmental factors, increases the risk for development of severe rsv infection. the purpose of this study was to describe differences in risk factors and protective factors between preterm birth (up to 35 weeks' gestational age) and term infants hospitalized for rsv lower respiratory tract infection in the russian federation during the 200 ... | 2011 | 21792335 |
| effects of decabrominated diphenyl ether (dbde) on developmental immunotoxicity in offspring mice. | decabrominated diphenyl ether (dbde), a representative brominated flame retardant ubiquitous in the environment, is suspected of being hazardous to humans. we evaluated the developmental immunotoxicity of dbde by an assay system using respiratory syncytial virus (rsv) infection in offspring mice. pregnant mice were continuously exposed to dbde (10, 100, 1000, or 10,000ppm) in the diet from gestation day 10 to weaning on postnatal day 21. offspring mice born to these dams were intranasally infect ... | 2008 | 21791381 |
| a novel inactivated intranasal respiratory syncytial virus vaccine promotes viral clearance without th2 associated vaccine-enhanced disease. | respiratory syncytial virus (rsv) is a leading cause of bronchiolitis and pneumonia in young children worldwide, and no vaccine is currently available. inactivated rsv vaccines tested in the 1960's led to vaccine-enhanced disease upon viral challenge, which has undermined rsv vaccine development. rsv infection is increasingly being recognized as an important pathogen in the elderly, as well as other individuals with compromised pulmonary immunity. a safe and effective inactivated rsv vaccine wou ... | 2011 | 21789184 |
| a new assay system for evaluation of developmental immunotoxicity of chemical compounds using respiratory syncytial virus infection to offspring mice. | we evaluated the effect of 6-propyl-2-thiouracil (ptu), an anti-thyroid agent, on developmental immunity using respiratory syncytial virus (rsv) infection to offspring mice as a new risk assessment for brominated flame retardants (bfrs), because some bfrs are suspected of affecting the thyroid system. pregnant mice were exposed to ptu in drinking water from gestation day 10 to weaning on postnatal day 21. their offspring mice were infected intranasally with rsv. exposure of 100ppm ptu significan ... | 2008 | 21783838 |
| effect of ozone on susceptibility to respiratory viral infection and virus-induced cytokine secretion. | airway epithelium is the primary target tissue for respiratory viruses as well as an important target of ozone (o(3)) toxicity. a change in the severity of viral airway infection may result from changes in epithelial cell susceptibility to infection, metabolic interference with viral replication, or altered production of immune regulatory molecules by the infected cells as a result of exposure to o(3). in this study we have investigated whether o(3) exposure alters the susceptibility of human ai ... | 1998 | 21781902 |
| increased detection of respiratory viruses in paediatric outpatients with acute respiratory illness by real-time polymerase chain reaction using nasopharyngeal flocked swabs. | detection of respiratory viruses by realtime multiplexed pcr (m-pcr) and of rsv by m-pcr and immunofluorescence(if) was evaluated using specimens collected by nasopharyngeal flocked swab(nfs) and nasal wash(nw). in children with mild respiratory illness, nfs collection was superior to nw collection for detection of viruses by m-pcr (sensitivity 89.6% vs 79.2%, p=0.0043). nfs collection was non-inferior to nw collection in detecting rsv by if. | 2011 | 21775539 |
| clara cell protein 10 polymorphism is not associated with severe respiratory syncytial virus infection. | aim: to investigate a possible correlation between severe respiratory syncytial virus (rsv) infection and single-nucleotide polymorphism in the clara cell protein 10 (cc10) gene (a38g). methods: children hospitalized with severe rsv lower respiratory tract (ltri) infection at karolinska university hospital stockholm during three subsequent rsv seasons were selected and genotyped. age-matched controls were used. the two groups were compared regarding single nucleotide polymorphism in the cc10 g ... | 2011 | 21767304 |
| characterization of 2009 h1n1 pandemic influenza in a population of dutch children with influenza-like signs and symptoms. | aim: to determine causative respiratory pathogens and describe epidemiological and clinical characteristics in a paediatric population with influenza-like illness during the 2009 h1n1-pandemic. methods: observational study of 412 children visiting an outpatient clinic of a dutch teaching hospital. results: from august to december 2009, 412 children were tested at the clinic; 32% proved h1n1-positive, confirmed by reverse-transcriptase-polymerase-chain-reaction (rt-pcr). pathogens were detecte ... | 2011 | 21767303 |
| the role of rsv infection in asthma initiation and progression: findings in a mouse model. | respiratory syncytial virus (rsv) is a common cause of severe lower respiratory tract diseases (bronchiolitis and pneumonia) during infancy and early childhood. there is increasing evidence which indicates that severe pulmonary disease caused by rsv infection in infancy is associated with recurrent wheezing and development of asthma later in childhood. however, the underlying mechanisms linking rsv infection to persistent airway hyperresponsiveness and dysfunction are not fully defined. to study ... | 2011 | 21766019 |
| infection in immunocompromised hosts and the organ transplant recipient -essentials. | 2011 | 21748845 | |
| role of the fas/fasl system in a model of rsv infection in mechanically ventilated mice. | infection with respiratory syncytial virus (rsv) in children can progress to respiratory distress and acute lung injury (ali) necessitating mechanical ventilation (mv). mv enhances apoptosis and inflammation in mice infected with pneumonia virus of mice (pvm), a mouse pneumovirus that has been used as a model for severe rsv infection in mice. we hypothesized that the fas/fas ligand (fasl) system, a dual pro-apoptotic/pro-inflammatory system involved in other forms of lung injury, is required for ... | 2011 | 21743025 |
| respiratory syncytial virus binds and undergoes transcription in neutrophils from the blood and airways of infants with severe bronchiolitis. | neutrophils are the predominant cell in the lung inflammatory infiltrate of infants with respiratory syncytial virus (rsv) bronchiolitis. although it has previously been shown that neutrophils from both blood and bronchoalveolar lavage (bal) are activated, little is understood about their role in response to rsv infection. this study investigated whether rsv proteins and mrna are present in neutrophils from blood and bal of infected infants. | 2011 | 21742845 |
| epidemiological study of hospitalization associated with respiratory syncytial virus infection in taiwanese children between 2004 and 2007. | respiratory syncytial virus (rsv) is the leading cause of hospitalization in young children. the population-based burden of rsv hospitalization and the effect of potential risk factors on the severity of illness were evaluated in taiwanese children. | 2011 | 21741007 |