Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| severe acute respiratory syndrome. | severe acute respiratory syndrome (sars) is an infectious disease first recognized in november 2002 in guangdong province, china. it spread to many countries all over the world during february to june 2003, with 8098 cases reported. twenty-one percent of the affected people were health care workers. because sars is a new emerging disease, this review describes the current understanding about the etiology, clinical pictures, laboratory and radiological findings of sars. | 2004 | 15021055 |
| analysis of multimerization of the sars coronavirus nucleocapsid protein. | severe acute respiratory syndrome (sars), an emerging disease characterized by atypical pneumonia, has recently been attributed to a novel coronavirus. the genome of sars coronavirus (sars-cov) has recently been sequenced, and a number of genes identified, including that of the nucleocapsid protein (n). it is noted, however, that the n protein of sars-cov (sars-cov n) shares little homology with nucleocapsid proteins of other members of the coronavirus family [science 300 (2003) 1399; science 30 ... | 2004 | 15020242 |
| evidence from the evolutionary analysis of nucleotide sequences for a recombinant history of sars-cov. | the origins and evolutionary history of the severe acute respiratory syndrome (sars) coronavirus (sars-cov) remain an issue of uncertainty and debate. based on evolutionary analyses of coronavirus dna sequences, encompassing an approximately 13kb stretch of the sars-tor2 genome, we provide evidence that sars-cov has a recombinant history with lineages of types i and iii coronavirus. we identified a minimum of five recombinant regions ranging from 83 to 863bp in length and including the polymeras ... | 2004 | 15019585 |
| sars: clinical features and diagnosis. | severe acute respiratory syndrome (sars) is a highly infectious disease with a significant morbidity and case fatality. the major clinical features include persistent fever, chills/rigor, myalgia, malaise, dry cough, headache and dyspnoea. less common symptoms include sputum production, sore throat, coryza, dizziness, nausea, vomiting and diarrhoea. older subjects may present with decrease in general well-being, poor feeding, fall/fracture and delirium, without the typical febrile response. comm ... | 2003 | 15018129 |
| sars: epidemiology. | severe acute respiratory syndrome (sars) originated in southern china in november 2002, and was brought to hong kong in february 2003. from hong kong, the disease spread rapidly worldwide but mostly to asian countries. at the end of the epidemic in june, the global cumulative total was 8422 cases with 916 deaths (case fatality rate of 11%). people of all ages were affected, but predominantly females. health care workers were at high risk and accounted for one-fifth of all cases. risk factors for ... | 2003 | 15018127 |
| sars: clinical virology and pathogenesis. | severe acute respiratory syndrome (sars) is caused by a novel coronavirus, called the sars coronavirus (sars-cov). over 95% of well characterized cohorts of sars have evidence of recent sars-cov infection. the genome of sars-cov has been sequenced and it is not related to any of the previously known human or animal coronaviruses. it is probable that sars-cov was an animal virus that adapted to human-human transmission in the recent past. the virus can be found in nasopharyngeal aspirate, urine a ... | 2003 | 15018126 |
| prior infection and passive transfer of neutralizing antibody prevent replication of severe acute respiratory syndrome coronavirus in the respiratory tract of mice. | following intranasal administration, the severe acute respiratory syndrome (sars) coronavirus replicated to high titers in the respiratory tracts of balb/c mice. peak replication was seen in the absence of disease on day 1 or 2, depending on the dose administered, and the virus was cleared within a week. viral antigen and nucleic acid were detected in bronchiolar epithelial cells during peak viral replication. mice developed a neutralizing antibody response and were protected from reinfection 28 ... | 2004 | 15016880 |
| identification of an hla-a*0201-restricted cd8+ t-cell epitope ssp-1 of sars-cov spike protein. | a novel coronavirus, severe acute respiratory syndrome (sars)-associated coronavirus (sars-cov), has been identified as the causal agent of sars. spike (s) protein is a major structural glycoprotein of the sars virus and a potential target for sars-specific cell-mediated immune responses. a panel of s protein-derived peptides was tested for their binding affinity to hla-a*0201 molecules. peptides with high affinity for hla-a*0201 were then assessed for their capacity to elicit specific immune re ... | 2004 | 15016646 |
| [some research clues on chinese herbal medicine for sars prevention and treatment]. | to provide some research clues from chinese herbal medicine for sars prevention and treatment. | 2003 | 15015319 |
| development of a western blot assay for detection of antibodies against coronavirus causing severe acute respiratory syndrome. | to identify a major antigenic determinant for use in the development of a rapid serological diagnostic test for severe acute respiratory syndrome (sars) coronavirus infection and to study the immune response during sars coronavirus infection in humans, we cloned the full length and six truncated fragments of the nucleocapsid gene, expressed them, and purified them as glutathione s-transferase-tagged recombinant proteins. the reactivities of the recombinant proteins to a panel of antibodies conta ... | 2004 | 15013997 |
| development of a western blot assay for detection of antibodies against coronavirus causing severe acute respiratory syndrome. | to identify a major antigenic determinant for use in the development of a rapid serological diagnostic test for severe acute respiratory syndrome (sars) coronavirus infection and to study the immune response during sars coronavirus infection in humans, we cloned the full length and six truncated fragments of the nucleocapsid gene, expressed them, and purified them as glutathione s-transferase-tagged recombinant proteins. the reactivities of the recombinant proteins to a panel of antibodies conta ... | 2004 | 15013997 |
| profiles of antibody responses against severe acute respiratory syndrome coronavirus recombinant proteins and their potential use as diagnostic markers. | a new coronavirus (severe acute respiratory syndrome coronavirus [sars-cov]) has been identified to be the etiological agent of severe acute respiratory syndrome. given the highly contagious and acute nature of the disease, there is an urgent need for the development of diagnostic assays that can detect sars-cov infection. for determination of which of the viral proteins encoded by the sars-cov genome may be exploited as diagnostic antigens for serological assays, the viral proteins were express ... | 2004 | 15013989 |
| recombinant protein-based enzyme-linked immunosorbent assay and immunochromatographic tests for detection of immunoglobulin g antibodies to severe acute respiratory syndrome (sars) coronavirus in sars patients. | an enzyme-linked immunosorbent assay (elisa) and a rapid immunochromatographic test for detection of immunoglobulin g (igg) antibodies in severe acute respiratory syndrome (sars) patients were developed by utilizing the well-characterized recombinant proteins gst-n and gst-u274. the elisa detected igg antibodies to sars-cov in all 74 convalescent-phase samples from sars patients while weakly cross-reacting to only 1 of the 210 control sera from healthy donors. this finding thus led to a kit sens ... | 2004 | 15013977 |
| characterization of severe acute respiratory syndrome-associated coronavirus (sars-cov) spike glycoprotein-mediated viral entry. | severe acute respiratory syndrome-associated coronavirus (sars-cov) is a rapidly emerging pathogen with potentially serious consequences for public health. here we describe conditions that result not only in the efficient expression of the sars-cov spike (s) protein on the surface of cells, but in its incorporation into lentiviral particles that can be used to transduce cells in an s glycoprotein-dependent manner. we found that although some primate cell lines, including vero e6, 293t and huh-7 ... | 2004 | 15010527 |
| the severe acute respiratory syndrome-coronavirus replicative protein nsp9 is a single-stranded rna-binding subunit unique in the rna virus world. | the recently identified etiological agent of the severe acute respiratory syndrome (sars) belongs to coronaviridae (cov), a family of viruses replicating by a poorly understood mechanism. here, we report the crystal structure at 2.7-a resolution of nsp9, a hitherto uncharacterized subunit of the sars-cov replicative polyproteins. we show that sars-cov nsp9 is a single-stranded rna-binding protein displaying a previously unreported, oligosaccharide/oligonucleotide fold-like fold. the presence of ... | 2004 | 15007178 |
| preclinical evaluation of two real-time, reverse transcription-pcr assays for detection of the severe acute respiratory syndrome coronavirus. | we verified the analytical performance characteristics of a previously described real-time reverse transcription-pcr (rt-pcr) assay targeting the open reading frame (orf) 1b region of the severe acute respiratory syndrome coronavirus (sars-cov) with rna transcripts. we then compared it to a novel nucleocapsid gene real-time rt-pcr assay with genomic rna. the assays differed only in the primer and probe sequences and final concentrations. a commercially available armored rna (ambion, austin, tex. ... | 2004 | 15004042 |
| [severe acute respiratory syndrome (sars)--present status]. | basic facts about sars are presented, including epidemiology, clinical symptoms and the course of disease, multinational search of etiological factor, diagnostic approaches and others. the role of molecular biology in elucidating structure, features and phylogenetic aspects of sars coronavirus is underlined. | 2003 | 15002168 |
| structural characterization of the sars-coronavirus spike s fusion protein core. | the spike (s) glycoprotein of coronaviruses mediates viral entry into host cells. it is a type 1 viral fusion protein that characteristically contains two heptad repeat regions, denoted hr-n and hr-c, that form coiled-coil structures within the ectodomain of the protein. previous studies have shown that the two heptad repeat regions can undergo a conformational change from their native state to a 6-helix bundle (trimer of dimers), which mediates fusion of viral and host cell membranes. here we d ... | 2004 | 14996844 |
| tracking the evolution of the sars coronavirus using high-throughput, high-density resequencing arrays. | mutations in the sars-coronavirus (sars-cov) can alter its clinical presentation, and the study of its mutation patterns in human populations can facilitate contact tracing. here, we describe the development and validation of an oligonucleotide resequencing array for interrogating the entire 30-kb sars-cov genome in a rapid, cost-effective fashion. using this platform, we sequenced sars-cov genomes from vero cell culture isolates of 12 patients and directly from four patient tissues. the sequenc ... | 2004 | 14993206 |
| the phylogeny of sars coronavirus. | different tree-building methods consistently place the sars corona-virus (sars-cov) as a basal group 2 coronavirus rather than as an ungrouped species as concluded by others. detailed comparisons of the sars-cov genomic sequence with those of six other coronaviruses failed to find evidence of recombination or genomic rearrangement using computational methods designed for that purpose. | 2004 | 14991447 |
| [to understand common pathogenic viruses in pediatrics from the perspectives of viral taxonomy]. | 2004 | 14990095 | |
| sars treatment. interferon shows promise in monkeys. | 2004 | 14988528 | |
| silencing sars-cov spike protein expression in cultured cells by rna interference. | the severe acute respiratory syndrome (sars) has been one of the most epidemic diseases threatening human health all over the world. based on clinical studies, sars-cov (the sars-associated coronavirus), a novel coronavirus, is reported as the pathogen responsible for the disease. to date, no effective and specific therapeutic method can be used to treat patients suffering from sars-cov infection. rna interference (rnai) is a process by which the introduced small interfering rna (sirna) could ca ... | 2004 | 14988013 |
| [atypical pathogens of pneumonia acquired in ambulant persons]. | 2004 | 14986239 | |
| characterization of humoral responses in mice immunized with plasmid dnas encoding sars-cov spike gene fragments. | the immunological characteristics of sars-cov spike protein were investigated by administering mice with plasmids encoding various s gene fragments. we showed that the secreting forms of s1, s2 subunits and the n-terminus of s1 subunit (residues 18-495) were capable of eliciting sars-cov specific antibodies and the region immediate to n-terminus of matured s1 protein contained an important immunogenic determinant for elicitation of sars-cov specific antibodies. in addition, mice immunized with p ... | 2004 | 14985131 |
| [slaughter of possible animal carriers of sars]. | 2004 | 14983210 | |
| characterization of severe acute respiratory syndrome coronavirus genomes in taiwan: molecular epidemiology and genome evolution. | since early march 2003, the severe acute respiratory syndrome (sars) coronavirus (cov) infection has claimed 346 cases and 37 deaths in taiwan. the epidemic occurred in two stages. the first stage caused limited familial or hospital infections and lasted from early march to mid-april. all cases had clear contact histories, primarily from guangdong or hong kong. the second stage resulted in a large outbreak in a municipal hospital, and quickly spread to northern and southern taiwan from late apri ... | 2004 | 14983045 |
| potent neutralization of severe acute respiratory syndrome (sars) coronavirus by a human mab to s1 protein that blocks receptor association. | effective prophylaxis and antiviral therapies are urgently needed in the event of reemergence of the highly contagious and often fatal severe acute respiratory syndrome (sars) coronavirus (sars-cov) infection. we have identified eight recombinant human single-chain variable region fragments (scfvs) against the s1 domain of spike (s) protein of the sars-cov from two nonimmune human antibody libraries. one scfv 80r efficiently neutralized sars-cov and inhibited syncytia formation between cells exp ... | 2004 | 14983044 |
| geldanamycin, a ligand of heat shock protein 90, inhibits the replication of herpes simplex virus type 1 in vitro. | geldanamycin (ga) is an antibiotic targeting the adp/atp binding site of heat shock protein 90 (hsp90). in screening for anti-herpes simplex virus type 1 (hsv-1) candidates, we found ga active against hsv-1. hsv-1 replication in vitro was significantly inhibited by ga with an 50% inhibitory concentration of 0.093 microm and a concentration that inhibited cellular growth 50% in comparison with the results seen with untreated controls of 350 microm. the therapeutic index of ga was over 3700 (compa ... | 2004 | 14982777 |
| pegylated interferon-alpha protects type 1 pneumocytes against sars coronavirus infection in macaques. | the primary cause of severe acute respiratory syndrome (sars) is a newly discovered coronavirus. replication of this sars coronavirus (scv) occurs mainly in the lower respiratory tract, and causes diffuse alveolar damage. lack of understanding of the pathogenesis of sars has prevented the rational development of a therapy against this disease. here we show extensive scv antigen expression in type 1 pneumocytes of experimentally infected cynomolgus macaques (macaca fascicularis) at 4 d postinfect ... | 2004 | 14981511 |
| pegylated interferon-alpha protects type 1 pneumocytes against sars coronavirus infection in macaques. | the primary cause of severe acute respiratory syndrome (sars) is a newly discovered coronavirus. replication of this sars coronavirus (scv) occurs mainly in the lower respiratory tract, and causes diffuse alveolar damage. lack of understanding of the pathogenesis of sars has prevented the rational development of a therapy against this disease. here we show extensive scv antigen expression in type 1 pneumocytes of experimentally infected cynomolgus macaques (macaca fascicularis) at 4 d postinfect ... | 2004 | 14981511 |
| [expression and purification of recombinant n-terminal protein of sars s1 subunit expressed in e. coli]. | to express and purify the recombinant n-terminal protein of sars virus s1 subunit and to study its role in sars immune response. | 2004 | 14970895 |
| [diagnostic tests approved by ministry of health and welfare (december 2003)]. | 2004 | 14968565 | |
| molecular cloning, expression, purification, and mass spectrometric characterization of 3c-like protease of sars coronavirus. | severe acute respiratory syndrome (sars) is an acute respiratory illness, which has broken out in china. it has been known that sars coronavirus (sars_cov) is a novel human coronavirus and is responsible for sars infection. belonging to one of the major proteins associated with sars_cov, sars 3c-like protease (sars_3cl(pro)) functions as a cysteine protease engaging in the proteolytic cleavage of the viral precursor polyprotein to a series of functional proteins required for coronavirus replicat ... | 2003 | 14965777 |
| medicine. caution urged on sars vaccines. | 2004 | 14963300 | |
| the nsp9 replicase protein of sars-coronavirus, structure and functional insights. | as part of a high-throughput structural analysis of sars-coronavirus (sars-cov) proteins, we have solved the structure of the non-structural protein 9 (nsp9). this protein, encoded by orf1a, has no designated function but is most likely involved with viral rna synthesis. the protein comprises a single beta-barrel with a fold previously unseen in single domain proteins. the fold superficially resembles an ob-fold with a c-terminal extension and is related to both of the two subdomains of the sars ... | 2004 | 14962394 |
| sars returns to china. | 2004 | 14959753 | |
| mild illness associated with severe acute respiratory syndrome coronavirus infection: lessons from a prospective seroepidemiologic study of health-care workers in a teaching hospital in singapore. | severe acute respiratory syndrome (sars) is a newly recognized infectious disease that has recently emerged in east asia and north america. although the clinical features of acute infection have been well described, mildly symptomatic or asymptomatic infections have not been well characterized. | 2004 | 14767817 |
| severe acute respiratory syndrome: developing a research response. | when severe acute respiratory syndrome (sars) first came to world attention in march 2003, it was immediately perceived to be a global threat with a pandemic potential. to help coordinate international research efforts, the national institute of allergy and infectious diseases convened a colloquium entitled sars: developing a research response on 30 may 2003. breakout sessions intended to identify unmet research needs in 5 areas of sars research--clinical research, epidemiology, diagnostics, the ... | 2004 | 14767816 |
| expression cloning of functional receptor used by sars coronavirus. | we have expressed a series of truncated spike (s) glycoproteins of sars-cov and found that the n-terminus 14-502 residuals were sufficient to bind to sars-cov susceptible vero e6 cells. with this soluble s protein fragment as an affinity ligand, we screened hela cells transduced with retroviral cdna library from vero e6 cells and obtained a hela cell clone which could bind with the s protein. this cell clone was susceptible to hiv/sars pseudovirus infection and the presence of a functional recep ... | 2004 | 14766227 |
| severe acute respiratory syndrome without respiratory symptoms or abnormal chest radiograph findings. | we report a serologically confirmed case of severe acute respiratory syndrome (sars) in a 27-year-old health care worker. the patient reported no respiratory complaints, and the findings of serial chest radiographs were normal. this case illustrates the wide spectrum of clinical illness caused by infection with sars coronavirus. | 2004 | 14765354 |
| viral replication in the nasopharynx is associated with diarrhea in patients with severe acute respiratory syndrome. | the role of severe acute respiratory syndrome (sars) coronavirus as an enteric pathogen was investigated in a cohort of 142 patients with sars who were treated with a standard treatment protocol. data from daily hematological, biochemical, radiological, and microbiological investigations were prospectively collected, and the correlation of these findings with diarrhea was retrospectively analyzed. sixty-nine patients (48.6%) developed diarrhea at a mean (+/- standard deviation [sd]) of 7.6+/-2.6 ... | 2004 | 14765337 |
| recognition and analysis of protein-coding genes in severe acute respiratory syndrome associated coronavirus. | the recent outbreak of severe acute respiratory syndrome (sars) caused by sars coronavirus (sars-cov) has necessitated an in-depth molecular understanding of the virus to identify new drug targets. the availability of complete genome sequence of several strains of sars virus provides the possibility of identification of protein-coding genes and defining their functions. computational approach to identify protein-coding genes and their putative functions will help in designing experimental protoc ... | 2004 | 14764577 |
| proteomic analysis on structural proteins of severe acute respiratory syndrome coronavirus. | recently, a new coronavirus was isolated from the lung tissue of autopsy sample and nasal/throat swabs of the patients with severe acute respiratory syndrome (sars) and the causative association with sars was determined. to reveal further the characteristics of the virus and to provide insight about the molecular mechanism of sars etiology, a proteomic strategy was utilized to identify the structural proteins of sars coronavirus (sars-cov) isolated from vero e6 cells infected with the bj-01 stra ... | 2004 | 14760722 |
| severe acute respiratory syndrome: public health response and clinical practice update for an emerging disease. | severe acute respiratory syndrome is an emerging infectious disease with high morbidity and mortality and potential for global spread. this review highlights the salient points of the 2002-2003 outbreak to help clinicians with early recognition, treatment, disease control, and prevention. | 2004 | 14758116 |
| [importance of nosocomial transmission on severe acute respiratory syndrome and its prevention]. | 2004 | 14756992 | |
| [sars (severe acute respiratory syndrome). emergent transmissible disease]. | of the reemergent transmissible diseases of the past decades, sars is probably not the last to express the alterations occurring in the relationships of the human being with its global ecosystem. the life of contemporary man is characterized, among others, by a huge thirst for traveling, for varied reasons, consequence of the globalization process. sars virus, mutant belonging to coronaviridae, occurred in one of the most densely populated areas of the world. there are two main moments marking t ... | 2003 | 14755924 |
| adolescent twin sisters with severe acute respiratory syndrome (sars). | a novel coronavirus-associated communicable respiratory disease, severe acute respiratory syndrome (sars), spread worldwide after an outbreak in guangdong province of the people's republic of china in november 2002. since late february 2003, there has been an epidemic in hong kong involving both adult and pediatric patients. the clinical course, intensive care, and outcome of adolescent twin sisters with sars are described. adolescents infected with sars may develop severe illness as adults, and ... | 2004 | 14754985 |
| molecular evolution of the sars coronavirus during the course of the sars epidemic in china. | sixty-one sars coronavirus genomic sequences derived from the early, middle, and late phases of the severe acute respiratory syndrome (sars) epidemic were analyzed together with two viral sequences from palm civets. genotypes characteristic of each phase were discovered, and the earliest genotypes were similar to the animal sars-like coronaviruses. major deletions were observed in the orf8 region of the genome, both at the start and the end of the epidemic. the neutral mutation rate of the viral ... | 2004 | 14752165 |
| antibody detection of sars-cov spike and nucleocapsid protein. | early detection and identification of sars-cov-infected patients and actions to prevent transmission are absolutely critical to prevent another sars outbreak. antibodies that specifically recognize the sars-cov spike and nucleocapsid proteins may provide a rapid screening method to allow accurate identification and isolation of patients with the virus early in their infection. for this reason, we raised peptide-induced polyclonal antibodies against sars-cov spike protein and polyclonal antibodie ... | 2004 | 14751221 |
| [etiology of severe acute respiratory syndrome]. | 2003 | 14749001 | |
| [pay attention to severe acute respiratory syndrome]. | 2003 | 14748986 | |
| class orders now possible under ontario's public health legislation. | in april 2003, the ontario legislature amended the province's public health legislation as part of a package of amendments related to the recent outbreak of severe acute respiratory syndrome (sars). although the amendments to the health protection and promotion act (hppa) were clearly designed to address emergency situations like sars, they may have unintended and negative consequences for people living with hiv/aids. | 2003 | 14746297 |
| conference report - ii. investigating new vaccines: sars. highlights from the viral vaccine meeting; october 25-28, 2003; barcelona, spain. | 2003 | 14745373 | |
| conference report - i. investigating new vaccines: ebola and hiv: highlights from the viral vaccine meeting; october 25-28, 2003; barcelona, spain. | 2003 | 14745371 | |
| [progresses in studies on sars-associated coronavirus]. | 2003 | 14744401 | |
| [the diagnosis and differential diagnosis of three suspect sars cases]. | 2003 | 14744392 | |
| [clinical analysis of pediatric sars cases in beijing]. | to study clinical characteristics of pediatric sars cases in beijing. | 2003 | 14744375 |
| tissue and cellular tropism of the coronavirus associated with severe acute respiratory syndrome: an in-situ hybridization study of fatal cases. | severe acute respiratory syndrome (sars) is a new human infectious disease with significant morbidity and mortality. the disease has been shown to be associated with a new coronavirus (sars-cov). the clinical and epidemiological aspects of sars have been described. moreover, the viral genome of sars-cov has been fully sequenced. however, much of the biological behaviour of the virus is not known and data on the tissue and cellular tropism of sars-cov are limited. in this study, six fatal cases o ... | 2004 | 14743497 |
| tnf-alpha inhibition for potential therapeutic modulation of sars coronavirus infection. | 2004 | 14741070 | |
| the difficulties of testing for sars. | 2004 | 14739441 | |
| wet markets--a continuing source of severe acute respiratory syndrome and influenza? | context: live-animal markets (wet markets) provide a source of vertebrate and invertebrate animals for customers in tropical and subtropical regions of the world. wet markets sell live poultry, fish, reptiles, and mammals of every kind. live-poultry markets (mostly chicken, pigeon, quail, ducks, geese, and a wide range of exotic wild-caught and farm-raised fowl) are usually separated from markets selling fish or red-meat animals, but the stalls can be near each other with no physical separation. ... | 2004 | 14738798 |
| wet markets--a continuing source of severe acute respiratory syndrome and influenza? | context: live-animal markets (wet markets) provide a source of vertebrate and invertebrate animals for customers in tropical and subtropical regions of the world. wet markets sell live poultry, fish, reptiles, and mammals of every kind. live-poultry markets (mostly chicken, pigeon, quail, ducks, geese, and a wide range of exotic wild-caught and farm-raised fowl) are usually separated from markets selling fish or red-meat animals, but the stalls can be near each other with no physical separation. ... | 2004 | 14738798 |
| analysis of deaths during the severe acute respiratory syndrome (sars) epidemic in singapore: challenges in determining a sars diagnosis. | an outbreak of severe acute respiratory syndrome (sars), an infectious disease attributed to a novel coronavirus, occurred in singapore during the first quarter of 2003 and led to 204 patients with diagnosed illnesses and 26 deaths by may 2, 2003. twenty-one percent of these patients required admission to the medical intensive care unit. during this period, the center for forensic medicine, health sciences authority, singapore, performed a total of 14 postmortem examinations for probable and sus ... | 2004 | 14736283 |
| canadian researchers testing sars vaccine in china. | 2004 | 14734423 | |
| [clinical and chest x-ray characteristics of 5 cases with severe acute respiratory syndrome in children in shenzhen area]. | to explore clinical and chest x-ray features of sars in children to facilitate correct diagnosis. | 2003 | 14733797 |
| [sars-associated coronavirus gene fragments were detected from a suspected pediatric sars patient]. | a special "fever and cough" clinic was set up at the children's hospital affiliated to capital institute of pediatrics for children with symptoms of fever and cough in late april when the severe acute respiratory syndrome (sars) epidemic was at its peak in beijing to separate the children with fever from others during their visit to the outpatient department. | 2003 | 14733796 |
| molecular biological analysis of genotyping and phylogeny of severe acute respiratory syndrome associated coronavirus. | sars-cov is the causative agent of severe acute respiratory syndrome (sars) which has been associated with outbreaks of sars in guangdong, hong kong and beijing of china, and other regions worldwide. sars-cov from human has shown some variations but its origin is still unknown. the genotyping and phylogeny of sars-cov were analyzed and reported in this paper. | 2004 | 14733771 |
| sars: lessons learned from other coronaviruses. | the identification of a new coronavirus as the etiological agent of severe acute respiratory syndrome (sars) has evoked much new interest in the molecular biology and pathogenesis of coronaviruses. this review summarizes present knowledge on coronavirus molecular biology and pathogenesis with particular emphasis on mouse hepatitis virus (mhv). mhv, a member of coronavirus group 2, is a natural pathogen of the mouse; mhv infection of the mouse is considered one of the best models for the study of ... | 2003 | 14733734 |
| dna vaccine of sars-cov s gene induces antibody response in mice. | the spike (s) protein, a main surface antigen of sars-coronavirus (sars-cov), is one of the most important antigen candidates for vaccine design. in the present study, three fragments of the truncated s protein were expressed in e.coli, and analyzed with pooled sera of convalescence phase of sars patients. the full length s gene dna vaccine was constructed and used to immunize balb/c mice. the mouse serum igg antibody against sars-cov was measured by elisa with e. coli expressed truncated s prot ... | 2004 | 14732873 |
| understanding sars with wolfram approach. | stepping acquired immunodeficiency syndrome (aids), severe acute respiratory syndrome (sars) as another type of disease has been threatening mankind since late last year. many scientists worldwide are making great efforts to study the etiology of this disease with different approaches. 13 species of sars virus have been sequenced. however, most people still largely rely on the traditional methods with some disadvantages. in this work, we used wolfram approach to study the relationship among sars ... | 2004 | 14732867 |
| early detection of antibodies against various structural proteins of the sars-associated coronavirus in sars patients. | severe acute respiratory syndrome (sars), a new disease with symptoms similar to those of atypical pneumonia, raised a global alert in march 2003. because of its relatively high transmissibility and mortality upon infection, probable sars patients were quarantined and treated with special and intensive care. therefore, instant and accurate laboratory confirmation of sars-associated coronavirus (sars-cov) infection has become a worldwide interest. for this need, we purified recombinant proteins i ... | 2004 | 14730215 |
| infectious diseases. viral dna match spurs china's civet roundup. | 2004 | 14726558 | |
| molecular epidemiology of the novel coronavirus that causes severe acute respiratory syndrome. | severe acute respiratory syndrome (sars) is a newly emerged disease caused by a novel coronavirus (sars-cov), which spread globally in early 2003, affecting over 30 countries. we have used molecular epidemiology to define the patterns of spread of the virus in hong kong and beyond. | 2004 | 14726162 |
| asymptomatic severe acute respiratory syndrome-associated coronavirus infection. | 2003 | 14725258 | |
| antibodies to sars-like virus hint at repeated infections. | 2004 | 14724600 | |
| sars: retrospective cohort study among german guests of the hotel 'm', hong kong. | hong kong played a pivotal role in the international spread of the severe acute respiratory syndrome (sars): a doctor who spent the night of 21-22 february 2003 at hotel 'm' was identified as the index case for four national and international clusters of sars. in parallel to the international collaborative study led by who and united states, a retrospective study on the cohort of german persons staying at hotel 'm' was conducted. the inclusion criteria covered a period from 21 february to 3 marc ... | 2003 | 14724331 |
| early influenza in europe and sars escaping from high security laboratories! | 2003 | 14724330 | |
| [amplification and cloning of the n gene of sars-associated coronavirus]. | to amplify and clone the n gene of severe acute respiratory syndrome-associated coronavirus. | 2004 | 14724092 |
| preparation and characterization of monoclonal antibodies against s1 domain at n-terminal residues 249 to 667 of sars-associated coronavirus s1 protein. | to prepare and characterize monoclonal antibodies (mabs) against s1 protein of severe acute respiratory syndrome (sars)-associated coronavirus (sars-cov). | 2004 | 14724082 |
| [consensus of the management of severe acute respiratory syndrome]. | 2003 | 14723215 | |
| severe acute respiratory syndrome coronavirus sequence characteristics and evolutionary rate estimate from maximum likelihood analysis. | 2004 | 14722315 | |
| detection of severe acute respiratory syndrome coronavirus in blood of infected patients. | severe acute respiratory syndrome (sars) has caused major outbreaks worldwide, resulting in an urgent need to obtain sensitive and accurate diagnosis of this disease. pcr-based detection methods were developed for use on a variety of samples, including blood. eighteen subjects were investigated, and results indicated that blood samples contain sufficient virus for detection by using quantitative real-time pcr. | 2004 | 14715775 |
| profile of antibodies to the nucleocapsid protein of the severe acute respiratory syndrome (sars)-associated coronavirus in probable sars patients. | profiles of antibodies to the nucleocapsid protein of the severe acute respiratory syndrome (sars)-associated coronavirus in 445 probable sars patients and 3,749 healthy people or non-sars patients were analyzed by antigen-capturing enzyme-linked immunosorbent assay. antinucleocapsid antibodies were elucidated in 17.5% of the probable sars patients 1 to 7 days after the onset of symptoms and in 80% of the patients 8 to 14 days after the onset. about 90% of the probable sars patients were positiv ... | 2004 | 14715574 |
| a model of the ace2 structure and function as a sars-cov receptor. | the angiotensin-converting enzyme 2 (ace2) is an important regulator of the renin-angiotensin system and was very recently identified as a functional receptor for the sars virus. the ace2 sequence is similar (sequence identities 43% and 35%, and similarities 61% and 55%, respectively) to those of the testis-specific form of ace (tace) and the drosophila homolog of ace (ance). the high level of sequence similarity allowed us to build a robust homology model of the ace2 structure with a root-mean- ... | 2004 | 14715271 |
| swift response greets return of sars in china. | 2004 | 14712242 | |
| development of a sars vaccine: an industrial perspective on the global race against a global disease. | 2003 | 14711331 | |
| genomic sequencing of a sars coronavirus isolate that predated the metropole hotel case cluster in hong kong. | 2004 | 14709660 | |
| detection of sars coronavirus in patients with severe acute respiratory syndrome by conventional and real-time quantitative reverse transcription-pcr assays. | a novel coronavirus (cov) was recently identified as the agent for severe acute respiratory syndrome (sars). we compared the abilities of conventional and real-time reverse transcription-pcr (rt-pcr) assays to detect sars cov in clinical specimens. | 2004 | 14709637 |
| a genomics-based approach to biodefence preparedness. | 2004 | 14708013 | |
| antigenicity and receptor-binding ability of recombinant sars coronavirus spike protein. | severe acute respiratory syndrome (sars) is an emerging infectious disease associated with a novel coronavirus and causing worldwide outbreaks. sars coronavirus (sars-cov) is an enveloped rna virus, which contains several structural proteins. among these proteins, spike (s) protein is responsible for binding to specific cellular receptors and is a major antigenic determinant, which induces neutralizing antibody. in order to analyze the antigenicity and receptor-binding ability of sars-cov s prot ... | 2004 | 14706633 |
| infectious diseases. second lab accident fuels fears about sars. | 2004 | 14704402 | |
| [clinical analysis of patients with severe acute respiratory syndrome in beijing area]. | to study the epidemiological, clinical and laboratory characteristics, treatment and prognosis of patients with severe acute respiratory syndrome (sars). | 2003 | 14703443 |
| sars--lessons from two singapore general hospital cases. | two cases of severe acute respiratory syndrome (sars) that occurred in singapore general hospital are described. the lessons learnt are outlined. | 2003 | 14700422 |
| crouching tiger, hidden dragon: the laboratory diagnosis of severe acute respiratory syndrome. | 2004 | 14699466 | |
| sensitive and quantitative detection of severe acute respiratory syndrome coronavirus infection by real-time nested polymerase chain reaction. | a quantitative, real-time, nested polymerase chain reaction (pcr) method, combining the high sensitivity of nested pcr with time-saving real-time instrumentation, was developed for large-scale screening for severe acute coronavirus (sars) coronavirus. forty-six clinical specimens were analyzed by this method, and results were compared with those obtained by conventional, single-round, real-time reverse-transcriptase pcr (rt-pcr) performed in parallel. of the 17 positive results, 2 identified by ... | 2004 | 14699465 |
| an indolent case of severe acute respiratory syndrome. | severe acute respiratory syndrome (sars) is a highly contagious and typically rapidly progressive form of atypical pneumonia, which spread from asia to many parts of the world in early 2003. clinical diagnosis of sars requires the presence of unremitting fever and progressive pneumonia despite antibiotic therapy, particularly in the presence of lymphopenia and raised transaminase levels. we report the case of a woman who had undergone a successful allogeneic bone marrow transplant for acute myel ... | 2004 | 14695107 |
| canadian severe acute respiratory syndrome (sars) experience. | 2003 | 14694900 | |
| the virus that changed my world. | 2003 | 14691538 | |
| sars 3: are we ready? | 2003 | 14690300 |