Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| down syndrome and hospitalizations due to respiratory syncytial virus: a population-based study. | objective: to assess the risk estimates for respiratory syncytial virus (rsv) hospitalization in children with down syndrome (ds) and the clinical features and severity of rsv lower respiratory tract infection (lrti) in hospitalized children. study design: statewide hospitalization data for children with ds for 1995 through 2006 from the colorado health and hospital association database were combined with birth data from the colorado department of public health and environment to obtain populati ... | 2011 | 22177993 |
| [Etiology survey on virus of acute respiratory infection in Guangzhou from 2006 to 2009]. | To investigate the pathogens of acute respiratory infection (ARI) in Guangzhou from 2006 to 2009. | 2011 | 22177307 |
| [Detection of bovine respiratory syncytial virus by RT-PCR]. | The paper presents the results of studying the diagnostic efficiency of RT-PCR for the detection of respiratory syncytial virus in cattle of different ages. Glycoprotein F gene sequences were used as a target for amplification. The sensitivity of the reaction was 10 TCD50/ml and the virus detection rate in biomaterials averaged 19%. samples. That in RT-PCR correlated with the presence of clinical signs in sick animals. | 2011 | 22171476 |
| natural killer cells are involved in acute lung immune injury caused by respiratory syncytial virus infection. | it is known that respiratory syncytial virus (rsv) is the main cause of bronchiolitis and pneumonia in young children. rsv infection often leads to severe acute lung immunopathology, but the underlying immune mechanisms are less elucidated. here, we found that rsv infection induced severe acute lung immune injury and promoted the accumulation and activation of lung natural killer (nk) cells at the early stage of infection in balb/c mice. activated lung nk cells highly expressed activating recept ... | 2011 | 22171263 |
| performance on a multipathogen external quality assessment (eqa) panel by different mono- and multiplex nucleic acid amplification tests. | an external quality assessment (eqa) panel consisting of a total of 48 samples in broncho alveolar lavage (bal) or transport medium was prepared in collaboration with quality control for molecular diagnostics (qcmd) (www.qcmd.org). the panel was used to assess the proficiency of the 3 laboratories that would be responsible to examine the 6000 samples to be collected in the grace network of excellence (www.grace-lrti.org). the main objective was to decide on the best performing testing approach f ... | 2011 | 22170925 |
| The innate antiviral factor APOBEC3G targets replication of measles, mumps, and respiratory syncytial virus. | The cytidine deaminase APOBEC3G (apolipoprotein B mRNA-editing enzyme-catalytic polypeptide 3G; A3G) exerts antiviral activity against retroviruses, hepatitis B virus, adeno-associated virus, and transposable elements. We assessed whether the negative-strand (-)RNA viruses measles (MV), mumps (MuV), and respiratory syncytial virus (RSV) might be affected by A3G, and found that their infectivity was reduced by 1-2 logs (90-99%) in A3G over-expressing Vero cells, and in T cell lines expressing A3G ... | 2011 | 22170635 |
| increased replication of respiratory syncytial virus in the presence of cytokeratin 8 and 18. | previously, it was reported that productive viral infection, viral protein synthesis, and viral rna replication of respiratory syncytial virus (rsv) operated efficiently in two human epithelial cell lines (hep-2 and a549), but not in a human mast-cell line, hmc-1. based on these observations, it was hypothesized that hmc-1 cells lack the machinery required for rsv replication. to identify the host factors required for rsv replication, cdna subtraction using a549, hep-2, and hmc-1 cells was perfo ... | 2012 | 22170560 |
| airway ifn-γ production during rsv bronchiolitis is associated with eosinophilic inflammation. | study objective: this study was designed to investigate the possible role of ifn-γ in eosinophil degranulation that occurs during respiratory syncytial virus (rsv) bronchiolitis. methods: sixty-seven infants, 2-24 months old and hospitalized with their first episode of acute rsv bronchiolitis, were selected for this study. eosinophil-active cytokine and chemokine profiles in nasal lavage supernatants taken within ... | 2011 | 22160185 |
| Neonatal CD8 T-cell Hierarchy Is Distinct from Adults and Is Influenced by Intrinsic T cell Properties in Respiratory Syncytial Virus Infected Mice. | Following respiratory syncytial virus infection of adult CB6F1 hybrid mice, a predictable CD8+ T cell epitope hierarchy is established with a strongly dominant response to a K(d)-restricted peptide (SYIGSINNI) from the M2 protein. The response to K(d)M2(82-90) is ~5-fold higher than the response to a subdominant epitope from the M protein (NAITNAKII, D(b)M(187-195)). After infection of neonatal mice, a distinctly different epitope hierarchy emerges with codominant responses to K(d)M2(82-90) and ... | 2011 | 22144888 |
| Depletion of alveolar macrophages prolongs survival in response to acute pneumovirus infection. | Alveolar macrophages are immunoregulatory effector cells that interact directly with respiratory virus pathogens in vivo. We examined the role of alveolar macrophages in acute infection with pneumonia virus of mice (PVM), a rodent pneumovirus that replicates the clinical sequelae of severe human respiratory syncytial virus disease. We show that PVM replicates in primary mouse macrophage culture, releasing infectious virions and proinflammatory cytokines. Alveolar macrophages isolated from PVM-in ... | 2012 | 22129848 |
| applications of pcr (real-time and masstag) and enzyme-linked immunosorbent assay in diagnosis of respiratory infections and diarrheal illness among deployed u.s. military personnel during exercise balikatan 2009, philippines. | laboratory-based surveillance for diarrheal and respiratory illness was conducted at the 2009 republic of the philippines-united states balikatan exercise to determine the presence of specific pathogens endemic in the locations where the military exercises were conducted. ten stool and 6 respiratory specimens were obtained from individuals meeting case definitions for diarrhea or respiratory illness. stool specimens were frozen in dry ice and remotely tested using enzyme-linked immunosorbent ass ... | 2011 | 22128641 |
| Induction of mucosal and systemic immunity against respiratory syncytial virus by inactivated virus supplemented with TLR9 and NOD2 ligands. | Respiratory syncytial virus (RSV) infection is the most important viral cause of severe respiratory disease in infants and children worldwide and also forms a serious threat in the elderly. The development of RSV vaccine, however, has been hampered by the disastrous outcome of an earlier trial using an inactivated and parenterally administered RSV vaccine which did not confer protection but rather primed for enhanced disease upon natural infection. Mucosal administration does not seem to prime f ... | 2012 | 22120195 |
| Ge-Gen-Tang has anti-viral activity against human respiratory syncytial virus in human respiratory tract cell lines. | Ge-Gen-Tang (GGT) has been used against adult respiratory tract infection for thousand years in ancient China. However, GGT is unable to inhibit influenza virus. The effect of GGT to manage respiratory tract viral infection has been questioned. Several ingredients of GGT and their constituents are able to inhibit various viruses. Therefore, GGT might have antiviral activity against other viruses causing respiratory tract illness. Human respiratory syncytial virus (HRSV) is one of the most import ... | 2012 | 22120014 |
| isolation of influenza viruses in mdck 33016pf cells and clearance of contaminating respiratory viruses. | this paper summarizes results obtained by multiplex pcr screening of human clinical samples for respiratory viruses and corresponding data obtained after passaging of virus-positive samples in mdck 33016pf cells. using the resplexii v2.0 (qiagen) multiplex pcr, 393 positive results were obtained in 468 clinical samples collected during an influenza season in germany. the overall distribution of positive results was influenza a 42.0%, influenza b 38.7%, adenovirus 1.5%, bocavirus 0.5%, coronaviru ... | 2012 | 22119922 |
| A sensitive real-time PCR for detection and subgrouping of human respiratory syncytial virus. | Improved diagnostic tools for rapid detection, quantitation, and subgrouping of human respiratory syncytial virus (RSV) are needed to aid the development and evaluation of novel intervention strategies. A quantitative real-time RT-PCR using specific locked nucleic acid (LNA) probes was developed to identify RSV and to distinguish RSV subgroups A and B (RSV LNA assay). RSV subgroup diversity and the relationship between viral load and disease severity in confirmed RSV infections were also explore ... | 2012 | 22119628 |
| sendai virus-based rsv vaccine protects african green monkeys from rsv infection. | respiratory syncytial virus (rsv) is a serious disease of children, responsible for an estimated 160,000 deaths per year worldwide. despite the ongoing need for global prevention of rsv and decades of research, there remains no licensed vaccine. sendai virus (sev) is a mouse parainfluenza virus-type 1 which has been previously shown to confer protection against its human cousin, human parainfluenza virus-type 1 in african green monkeys (agm). here is described the study of a rsv vaccine (sevrsv) ... | 2011 | 22119594 |
| high frequency of human bocavirus 1 dna in infants and adults with lower acute respiratory infection. | human bocavirus (hbov) is a parvovirus with a possible etiological role in respiratory disease currently under investigation. we detected hbov1 in children and adults hospitalized with acute disease of the lower respiratory tract. hbov genome was detected by pcr in nasopharyngeal swabs collected from 75 patients aged 0-89 years old during 2010. hbov was found in 17/75 (22.7%) patients, 64.7% of them infants younger than 1 year old and 29.4% adults older than 30 years (the bimodal age distribu ... | 2011 | 22116985 |
| Picornavirus - the Most Common Respiratory Virus Infection among Patients of All Ages Hospitalized with Acute Respiratory Illness. | We evaluated the prevalence of respiratory virus infection (RVI) in 403 illnesses of persons hospitalized over a 2-year period with acute respiratory conditions using virus-specific RT-PCR assays in addition to cell culture and serology. RVIs were identified in >75% of children under 5 years and 25-37% of adults. The molecular assays doubled the number of infections identified; picornaviruses were the most frequent in patients of all ages, followed by respiratory syncytial virus and influenza vi ... | 2011 | 22116142 |
| antiviral activity in vitro of two preparations of the herbal medicinal product sinupret® against viruses causing respiratory infections. | sinupret(®), a herbal medicinal product made from gentian root, primula flower, elder flower, sorrel herb, and verbena herb is frequently used in the treatment of acute and chronic rhinosinusitis and respiratory viral infections such as common cold. to date little is known about its potential antiviral activity. therefore experiments have been performed to measure the antiviral activity of sinupret(®) oral drops (hereinafter referred to as "oral drops") and sinupret(®) dry extract (hereinafter r ... | 2011 | 22112724 |
| mycoplasma pneumoniae respiratory tract infections among greek children. | background: m. pneumoniae is a common cause of respiratory tract infections (rtis) of variable severity especially in children. new diagnostic techniques offered more reliable information about the epidemiology of infection by this pathogen.aim: the aim of this study was to investigate the prevalence and epidemiology of acute m. pneumoniae infections among greek children hospitalized for rtis using more advanced techniques.material and methods: the study included 225 greek children hospitalized ... | 2011 | 22110297 |
| seasonal variations in clostridium difficile infections are associated with influenza and respiratory syncytial virus activity independently of antibiotic prescriptions: a time series analysis in quebec, canada. | seasonal variations in clostridium difficile-associated diarrhea (cdad), with a higher incidence occurring during winter months, have been reported. although winter epidemics of respiratory viruses may be temporally associated with an increase in cdad morbidity, we hypothesized that this association is mainly due to increased antibiotic use for respiratory infections. the objective of this study was to evaluate the effect of the two most frequent respiratory viruses (influenza virus and respirat ... | 2011 | 22106208 |
| Slowing down with age: lung DCs do it too. | Decline in immune function with age has been attributed to defects or alterations in both the innate and the adaptive immune system. In this issue of the JCI, Zhao and coworkers provide evidence for a novel mechanism of immune dysfunction in aging mice. They show that migration of respiratory DCs from the site of virus replication to the draining lymph nodes in response to infection with several different respiratory viruses is markedly diminished with increasing age. The impaired DC migration w ... | 2011 | 22105176 |
| age-related increases in pgd(2) expression impair respiratory dc migration, resulting in diminished t cell responses upon respiratory virus infection in mice. | the morbidity and mortality associated with respiratory virus infection is felt most keenly among the elderly. t cells are necessary for viral clearance, and many age-dependent intrinsic t cell defects have been documented. however, the development of robust t cell responses in the lung also requires respiratory dcs (rdcs), which must process antigen and migrate to draining lns (dlns), and little is known about age-related defects in these t cell-extrinsic functions. here, we show that increases ... | 2011 | 22105170 |
| [aetiology of bronchiolitis in hospitalised children in south-east spain.] | background: bronchiolitis is the most common respiratory disease in children under 2 years-old and a major cause of hospitalization in young children, especially during the winter. objectives: to determine the prevalence and etiology of bronchiolitis in south-east of spain. study design: a prospective study was conducted during the bronchiolitis season (december-april). children below 18 months-old admitted to the hospital for a first bronchiolitis episode were included. nasopharyngeal aspirates ... | 2011 | 22104022 |
| Structures of respiratory syncytial virus nucleocapsid protein from two crystal forms: details of potential packing interactions in the native helical form. | Respiratory syncytial virus (RSV) is a frequent cause of respiratory illness in infants, but there is currently no vaccine nor effective drug treatment against this virus. The RSV RNA genome is encapsidated and protected by a nucleocapsid protein; this RNA-nucleocapsid complex serves as a template for viral replication. Interest in the nucleocapsid protein has increased owing to its recent identification as the target site for novel anti-RSV compounds. The crystal structure of human respiratory ... | 2011 | 22102022 |
| potent anti-respiratory syncytial virus activity of a cholestanol-sulfated tetrasaccharide conjugate. | a number of different viruses including respiratory syncytial virus (rsv) initiate infection of cells by binding to cell surface glycosaminoglycans and sulfated oligo- and polysaccharide mimetics of these receptors exhibit potent antiviral activity in cultured cells. we investigated whether the introduction of different lipophilic groups to the reducing end of sulfated oligosaccharides would modulate their anti-rsv activity. our results demonstrate that the cholestanol-conjugated tetrasaccharide ... | 2012 | 22101246 |
| association of herd bhv-1 seroprevalence with respiratory disease in youngstock in estonian dairy cattle. | the associations between herd bovine herpesvirus 1 (bhv-1) seroprevalence, along with other infectious and farm management factors with bovine respiratory disease (brd) in dairy calves and heifers, were investigated. serum samples from 103 dairy cattle herds were analyzed for antibodies against bhv-1, bovine respiratory syncytial virus (brsv), bovine viral diarrhea virus (bvdv), and mycoplasma bovis (m. bovis). a questionnaire was used to record herd management practices. a high occurrence of re ... | 2011 | 22100246 |
| serosurvey of bacterial and viral respiratory pathogens among deployed u.s. service members. | respiratory illnesses can cause substantial morbidity during military deployments. bordetella pertussis, chlamydia pneumoniae, mycoplasma pneumoniae, adenovirus, parainfluenza, and respiratory syncytial virus (rsv) are hypothesized causes. | 2011 | 22099233 |
| clinical and radiological features of respiratory syncytial virus in solid organ transplant recipients: a single-center experience. | background: respiratory syncytial virus (rsv) infections range from upper respiratory illness to severe lower respiratory disease. there is no universally accepted treatment for rsv in solid organ transplant (sot) recipients. methods: retrospective review of adult sot patients with rsv infections, between january 2007 and december 2009, in a single transplant center was performed. results: during the 3-year period, a total of 24 adults developed rsv infection, including 12 (50%) sot recipients ( ... | 2011 | 22093238 |
| low neonatal toll-like receptor 4-mediated interleukin-10 production is associated with subsequent atopic dermatitis. | atopic dermatitis (ad) and respiratory syncytial virus lower respiratory tract infection (rsv lrti) are common diseases during early life. impaired th1-cell polarizing toll-like receptor (tlr) responses play an important role in the pathogenesis of both diseases. neonatal tlr-mediated production of th1-type cytokines is decreased at birth, but rapidly increases during the first month of life. | 2011 | 22092594 |
| Cholesterol-rich Microdomains as Docking Platforms for Respiratory Syncytial Virus in Normal Human Bronchial Epithelial Cells. | Respiratory syncytial virus (RSV) is one of the major causes of respiratory infections in children and it is the main pathogen causing bronchiolitis in infants. The binding and entry mechanism by which RSV infects respiratory epithelial cells has not yet been determined. In this study, the earliest stages of RSV infection in normal human bronchial epithelial cells were probed by tracking virions with fluorescent lipophilic dyes in their membranes. Virions colocalized with cholesterol-containing ... | 2011 | 22090136 |
| respiratory syncytial virus glycoprotein g interacts with dc-sign and l-sign to activate erk1 and erk2. | respiratory syncytial virus (rsv) interaction with epithelial and dendritic cells (dcs) is known to require divalent cations, suggesting involvement of c-type lectins. rsv infection and maturation of primary human dcs are reduced in a dose-dependent manner by edta. therefore, we asked whether rsv infection involves dc-sign (cd209) or its isoform l-sign (cd299) (dc-sign/r). using surface plasmon resonance analysis, we demonstrated that the attachment g glycoprotein of rsv binds both dc- and l-sig ... | 2011 | 22090124 |
| Cholesterol-rich lipid rafts are required for release of infectious human respiratory syncytial virus particles. | Cholesterol and sphingolipid enriched lipid raft micro-domains in the plasma membrane play an important role in the life-cycle of numerous enveloped viruses. Although human respiratory syncytial virus (RSV) proteins associate with the raft domains of infected cells and rafts are incorporated in RSV virion particles, the functional role of raft during RSV infection was unknown. In the current study we have identified rafts as an essential component of host cell that is required for RSV infection. ... | 2012 | 22088217 |
| [respiratory syncytial virus infection in hematopoietic stem cell transplantation recipients with primary immunodeficiencies]. | to understand the clinical characteristics and outcome associated with respiratory syncytial virus (rsv) infection in hematopoietic stem cell transplantation (hsct) recipients with primary immunodeficiencies (pids). | 2011 | 22088176 |
| l-carbocisteine inhibits respiratory syncytial virus infection in human tracheal epithelial cells. | to examine the effects of l-carbocisteine on airway infection with respiratory syncytial (rs) virus, human tracheal epithelial cells were pretreated with l-carbocisteine and infected with rs virus. viral titer, virus rna, and pro-inflammatory cytokine secretion, including interleukin (il)-1 and il-6, increased with time after infection. l-carbocisteine reduced the viral titer in the supernatant fluids, the amount of rs virus rna, rs virus infection susceptibility, and the concentration of pro-in ... | 2012 | 22080978 |
| a respiratory syncytial virus isolate enables the testing of virucidal products. | the respiratory syncytial virus (rsv) is known as a major cause of respiratory infections and nosocomial diseases. testing this virus is rather difficult due to the problems encountered in producing it at a high titer without using any purification method. a rsv isolate which replicates to high level on a hep-2 cell line with an infectious titer of at least 10(7)tcid(50)ml(-1) in culture supernatant fluids has been identified. thanks to this isolate, the virucidal effects of two products, a hand ... | 2011 | 22079427 |
| effect of reduction in household air pollution on childhood pneumonia in guatemala (respire): a randomised controlled trial. | pneumonia causes more child deaths than does any other disease. observational studies have indicated that smoke from household solid fuel is a significant risk factor that affects about half the world's children. we investigated whether an intervention to lower indoor wood smoke emissions would reduce pneumonia in children. | 2011 | 22078686 |
| use of data linkage to investigate the aetiology of acute lower respiratory infection hospitalisations in children. | aim: to document the aetiology of acute lower respiratory infection (alri) hospitalisations in western australian children by linking population-based laboratory data with hospital morbidity data. methods: data from all alri hospitalisations and laboratory records related to respiratory pathogens between 2000 and 2005 were extracted and linked through a population-based record linkage system. the proportion of specimens that were positive for each respiratory viral or bacterial pathogen was do ... | 2011 | 22077532 |
| ten years of human metapneumovirus research. | described for the first time in 2001, human metapneumovirus (hmpv) has become one of the main viral pathogens responsible for acute respiratory tract infections in children but also in the elderly and immuno-compromised patients. the pathogen most closely related to hmpv is human respiratory syncytial virus (hrsv), the most common cause of bronchiolitis and pneumonia in young children. hmpv has been classified into two main viral groups a and b and has a seasonal distribution in temperate countr ... | 2011 | 22074934 |
| polyclonal immunoglobulins and hyperimmune globulins in prevention and management of infectious diseases. | immunoglobulin therapy has a rich history of use in preventing and treating infectious diseases; however, clinical data on the efficacy of immunoglobulin is lacking for many infectious diseases. immunoglobulin therapy is routinely used in postexposure prophylaxis for bacterial infections, including tetanus, botulism, and diphtheria, and viral infections, including hepatitis a and b and varicella. immunoglobulin therapy has also been used in many severe and life-threatening infections where treat ... | 2011 | 22054755 |
| Role of rhinovirus infections in asthma. | Human rhinoviruses are not only the main pathogens responsible for the common cold, but are now recognized to have a major impact on asthma pathogenesis. There is evidence that rhinovirus infections play a role in asthma development, asthma exacerbations and, potentially, airway remodeling. Children who experience repeated rhinovirus-induced wheezing episodes in infancy have a significantly increased risk of developing asthma, even when compared to children who experience wheezing induced by res ... | 2011 | 22053589 |
| Clinical evaluation of multiplex real-time PCR panels for rapid detection of respiratory viral infections. | Respiratory viral infections are one of the leading causes of morbidity and mortality, particularly in children, the elderly and immunocompromised persons. Rapid identification of viral etiology is critical in ruling out non-viral infections, initiating antiviral treatment and limiting the spread of the infection. Multiplex assays of more than one viral gene target in a single tube have the advantage of rapid screening of a large number of potential viral pathogens in a short time. A multiplex r ... | 2012 | 22052551 |
| evaluation of type-specific real-time pcr assays using the lightcycler and j.b.a.i.d.s. for detection of adenoviruses in species hadv-c. | sporadically, hadvs from species hadv-c are detected in acute respiratory disease outbreaks. to rapidly type these viruses, we designed real-time pcr assays that detect and discriminate between adenovirus types hadv-c1, -c2, -c5, and -c6. sixteen clinical isolates from the california department of public health were used to validate the new assays. type-specific taqman real-time pcr assays were designed and used independently to successfully identify 16 representative specimens. the lower limit ... | 2011 | 22046386 |
| the anti-apoptotic effect of respiratory syncytial virus on human peripheral blood neutrophils is mediated by a monocyte derived soluble factor. | respiratory syncytial virus (rsv) causes annual epidemics of respiratory disease particularly affecting infants. the associated airway inflammation is characterized by an intense neutrophilia. this neutrophilic inflammation appears to be responsible for much of the pathology and symptoms. previous work from our group had shown that there are factors within the airways of infants with rsv bronchiolitis that inhibit neutrophil apoptosis. this study was undertaken to determine if rsv can directly a ... | 2011 | 22046209 |
| [Antibody responses induced by mucosal DNA vaccine encoding the codon-optimized F protein of human respiratory syncytial virus (RSV) delivered with attenuated Salmonella typhimurium]. | In order to investigate antibody responses by musosal DNA vaccines encoding the codon-optimized F protein of human respiratory syncytial virus (RSV) delivered with attenuated Salmonella typhimurium aroA strain SL7207 (SL7207) via intranasal or intragastric routes. | 2011 | 22043798 |
| clinical and epidemiological characteristics of respiratory syncytial virus and influenza virus associated hospitalization in urban thai infants. | respiratory syncytial virus (rsv) and influenza infections are among the leading cause of hospitalized lower respiratory tract infections (lrti) in children especially among those younger than 1 year of age. few descriptions of these 2 important viruses in thai children less than 1 year of age have been published. | 2011 | 22043771 |
| dp2 (crth2) antagonism reduces ocular inflammation induced by allergen challenge and respiratory syncytial virus. | background: allergic conjunctivitis is characterized by itchy, watery and swollen eyes which occur in response to exposure to seasonal or environmental allergens. the early phase reaction of allergic conjunctivitis is primarily mediated by mast cell degranulation while the late phase reaction is driven by th2 cells and eosinophils. prostaglandin d(2) (pgd(2)), released from mast cells, is present in allergic conjunctival tears and may elicit classical allergic responses via interaction with the ... | 2011 | 22042170 |
| [chronology of a pandemic: the new influenza a (h1n1) in bogota, 2009-2010]. | describing the behaviour of the a h1n1/09 virus related to time, age and gender in patients having suspected infection, medical health care and laboratory diagnosis. | 2011 | 22031000 |
| relationship between the population incidence of febrile convulsions in young children in sydney, australia and seasonal epidemics of influenza and respiratory syncytial virus, 2003-2010: a time series analysis. | in 2010, intense focus was brought to bear on febrile convulsions in australian children particularly in relation to influenza vaccination. febrile convulsions are relatively common in infants and can lead to hospital admission and severe outcomes. we aimed to examine the relationships between the population incidence of febrile convulsions and influenza and respiratory syncytial virus (rsv) seasonal epidemics in children less than six years of age in sydney australia using routinely collected s ... | 2011 | 22029484 |
| inosine-containing rna is a novel innate immune recognition element and reduces rsv infection. | during viral infections, single- and double-stranded rna (ssrna and dsrna) are recognized by the host and induce innate immune responses. the cellular enzyme adar-1 (adenosine deaminase acting on rna-1) activation in virally infected cells leads to presence of inosine-containing rna (ino-rna). here we report that ss-ino-rna is a novel viral recognition element. we synthesized unmodified ssrna and ssrna that had 6% to16% inosine residues. the results showed that in primary human cells, or in mice ... | 2011 | 22028885 |
| Severe acute respiratory syndrome coronavirus envelope protein regulates cell stress response and apoptosis. | Severe acute respiratory syndrome virus (SARS-CoV) that lacks the envelope (E) gene (rSARS-CoV-?E) is attenuated in vivo. To identify factors that contribute to rSARS-CoV-?E attenuation, gene expression in cells infected by SARS-CoV with or without E gene was compared. Twenty-five stress response genes were preferentially upregulated during infection in the absence of the E gene. In addition, genes involved in signal transduction, transcription, cell metabolism, immunoregulation, inflammation, a ... | 2011 | 22028656 |
| a frequentist approach to estimating the force of infection for a respiratory disease using repeated measurement data from a birth cohort. | this article aims to develop a probability-based model involving the use of direct likelihood formulation and generalised linear modelling (glm) approaches useful in estimating important disease parameters from longitudinal or repeated measurement data. the current application is based on infection with respiratory syncytial virus. the force of infection and the recovery rate or per capita loss of infection are the parameters of interest. however, because of the limitation arising from the study ... | 2011 | 22028340 |
| Antiviral activity of arbidol, a broad-spectrum drug for use against respiratory viruses, varies according to test conditions. | The therapeutic activity of arbidol was investigated against representatives of seven different virus families. Its 50% median effective concentration (EC(50) ) was 0.22-11.8?µg/ml (0.41-22?nM). Therapeutic indices of 91 were obtained for type 1 poliovirus and 1.9-8.5 for influenza A and B, human paramyxo-3, avian infectious bronchitis-, and Marek's disease viruses. Arbidol was more inhibitory for influenza A/Aichi/2/68 (H3N2) virus than rimantadine or amantadine (EC(50) 10 vs. >15 and >31.6?µg/ ... | 2012 | 22028179 |
| Human bocavirus and other respiratory viral infections in a 2-year cohort of hospitalized children. | Human bocavirus (HBoV) infection is reported worldwide and may cause severe respiratory tract infections. The aim of the present study was to assess the prevalence of HBoV, and other respiratory viral pathogens, in a 2-year retrospective study of children admitted to hospital, and to investigate whether viral loads of HBoV DNA were associated with severity of infection. Between April 2007 and March 2009, 891 respiratory samples from 760 children admitted to hospital with acute respiratory tract ... | 2012 | 22028039 |
| Silver nanoparticles as potential antiviral agents. | Virus infections pose significant global health challenges, especially in view of the fact that the emergence of resistant viral strains and the adverse side effects associated with prolonged use continue to slow down the application of effective antiviral therapies. This makes imperative the need for the development of safe and potent alternatives to conventional antiviral drugs. In the present scenario, nanoscale materials have emerged as novel antiviral agents for the possibilities offered by ... | 2011 | 22024958 |
| CT of viral lower respiratory tract infections in adults: comparison among viral organisms and between viral and bacterial infections. | We retrospectively compared the CT findings of consecutive viral and bacterial lower respiratory tract infections (LRTIs) to determine their imaging appearance and any definable differences among the causative viruses and between the viral and bacterial infections. | 2011 | 22021500 |
| phosphorylation of the human respiratory syncytial virus n protein provokes a decrease in viral rna synthesis. | when hep-2 cells are infected by human respiratory syncytial virus (hrsv) its n protein becomes phosphorylated at tyrosine (y) y38, in a strictly regulated way. to determine how this phosphorylation affects nucleocapsid (nc) template activity during viral rna synthesis, n protein variants were analysed in which y38 and nearby y residues were substituted by phenylalanine (f; y23f, y38f and y69f) or aspartic acid (d; y23d and y38d). while the capacity of these proteins to form the nc and to intera ... | 2011 | 22019509 |
| Respiratory support by neurally adjusted ventilatory assist (NAVA) in severe RSV-related bronchiolitis: a case series report. | Neurally adjusted ventilatory assist (NAVA) is a new mode of mechanical ventilation controlled by diaphragmatic electrical signals. The electrical signals allow synchronization of ventilation to spontaneous breathing efforts of a child, as well as permitting pressure assistance proportional to the electrical signal. NAVA provides equally fine synchronization of respiratory support and pressure assistance varying with the needs of the child. NAVA has mainly been studied in children who underwent ... | 2011 | 22014152 |
| human rhinovirus species associated with hospitalizations for acute respiratory illness in young us children. | the contribution of human rhinovirus (hrv) to severe acute respiratory illness (ari) is unclear. | 2011 | 22013207 |
| severity of viral coinfection in hospitalized infants with respiratory syncytial virus infection. | 2011 | 22011744 | |
| impact of influenza during the post-pandemic season: epidemiological picture from syndromic and virological surveillance. | following the observation that 1 or 2 pandemic peak due to the circulation ofahinlv had occurred in most countries and in most world health organization (who) regions, who declared on august 10"h, 2010 that the world was moving into the post-pandemic period, whose surveillance presents considerable interest both from epidemiological and clinical point of view. we described the epidemiological picture emerged from syndromic and virological surveillance during the post-pandemic season in liguria, ... | 2011 | 22010543 |
| Respiratory syncytial virus--the unrecognised cause of health and economic burden among young children in Australia. | Respiratory syncytial virus (RSV) presents very similar to influenza and is the principle cause of bronchiolitis in infants and young children worldwide. Yet, there is no systematic monitoring of RSV activity in Australia. This study uses existing published data sources to estimate incidence, hospitalisation rates, and associated costs of RSV among young children in Australia. Published reports from the Laboratory Virology and Serology Reporting Scheme, a passive voluntary surveillance system, a ... | 2011 | 22010512 |
| specific viruses detected in nigerian children in association with acute respiratory disease. | occurrence of different viruses in acute respiratory tract infections of nigerian children was examined. respiratory swabs were collected from 246 children referred to hospital clinics because of acute respiratory symptoms from february through may 2009. validated real-time rt-pcr techniques revealed nucleic acids of at least one virus group in 189 specimens (77%). human rhinoviruses and parainfluenza viruses were present each in one third of the children. adenoviruses, enteroviruses, human meta ... | 2011 | 22007241 |
| respiratory syncytial virus (rsv) community-acquired pneumonia (cap) in a hospitalized adult with human immunodeficiency virus (hiv) mimicking influenza a and pneumocystis (carinii) jiroveci pneumonia (pcp). | respiratory syncytial virus (rsv) is an important cause of lower respiratory tract infections in young children, the elderly, and immunocompromised hosts, but rsv is a rare cause of community-acquired pneumonia (cap) in hospitalized adults with human immunodeficiency virus (hiv). in patients with hiv, cap is most frequently attributable to the usual bacterial respiratory pathogens that cause cap in immunocompetent hosts, eg, streptococcuspneumoniae or hemophilus influenzae. adults with hiv are a ... | 2012 | 22005289 |
| respiratory syncytial virus hospitalization trends in infants with chronic lung disease of infancy, 1998-2008. | infants with chronic lung disease of infancy (cldi) are at high risk for severe respiratory syncytial virus (rsv) illness requiring hospitalization. palivizumab was first licensed in 1998 for the prevention of rsv disease in high-risk infants, including those with cldi. we performed a retrospective cohort study of all hospitalized children with cldi aged <2 years between 1998 and 2008 in the usa to determine trends in rates of hospitalizations due to rsv (rsvh) since the launch of palivizumab. | 2011 | 22003308 |
| comparative dynamics, morbidity and mortality burden of pediatric viral respiratory infections in an equatorial city. | : although acute respiratory infections (aris) are the global leading cause of pediatric morbidity and mortality, the relative impact of viral pathogens on pediatric aris is still poorly understood, especially in equatorial settings. long-term studies of multiple viruses concurrently circulating in these regions are still lacking. here, we report the results of a systematic prospective surveillance of multiple respiratory viruses conducted every weekday for nearly a decade in an equatorial city ... | 2011 | 22001966 |
| whole genome sequencing and evolutionary analysis of human respiratory syncytial virus a and b from milwaukee, wi 1998-2010. | respiratory syncytial virus (rsv) is the leading cause of lower respiratory-tract infections in infants and young children worldwide. despite this, only six complete genome sequences of original strains have been previously published, the most recent of which dates back 35 and 26 years for rsv group a and group b respectively. | 2011 | 21998661 |
| nanobodies® specific for respiratory syncytial virus fusion protein protect against infection by inhibition of fusion. | despite the medical importance of respiratory syncytial virus (rsv) infections, there is no vaccine or therapeutic agent available. prophylactic administration of palivizumab, a humanized monoclonal rsv fusion (f) protein-specific antibody, can protect high-risk children. previously, we have demonstrated that rsv can be neutralized by picomolar concentrations of a camelid immunoglobulin single-variable domain that binds the rsv protein f (f-vhhb nanobodies). here, we investigated the mechanism b ... | 2011 | 21998474 |
| future opportunities for passive immunity against viral diseases. | 2011 | 21998473 | |
| comparison of the filmarray respiratory panel and prodesse real-time pcr assays for detection of respiratory pathogens. | we compared the diagnostic performance and overall respiratory pathogen detection rate of the premarket version of the filmarray respiratory panel (rp) multiplex pcr assay (idaho technology, inc., salt lake city, ut) with those of the food and drug administration (fda)-cleared prodesse proflu+, profast+, proparaflu+, pro hmpv+, and proadeno+ real-time pcr assays (gen-probe, san diego, ca). the assays were performed on a panel of 192 nasopharyngeal-secretion specimens collected from 81 children u ... | 2011 | 21998418 |
| renal transplant with bronchiolitis obliterans organizing pneumonia (boop) attributable to tacrolimus and herpes simplex virus (hsv) pneumonia. | background: solid organ transplants (sots) may be complicated by a wide variety of infectious and noninfectious pulmonary disorders. transplant patients receive immunosuppressive drugs to prevent rejection, but these drugs also predispose them to infection. because immunosuppressive therapy impairs t-lymphocyte function, ie, cell-mediated immunity, such therapy, not surprisingly, predisposes patients to intracellular pulmonary pathogens. community-acquired pneumonia (cap) in patients with sot us ... | 2011 | 21996615 |
| exploratory spatial analysis of in vitro respiratory syncytial virus co-infections. | the cell response to virus infection and virus perturbation of that response is dynamic and is reflected by changes in cell susceptibility to infection. in this study, we evaluated the response of human epithelial cells to sequential infections with human respiratory syncytial virus strains a2 and b to determine if a primary infection with one strain will impact the ability of cells to be infected with the second as a function of virus strain and time elapsed between the two exposures. infected ... | 2010 | 21994640 |
| nucleoproteins of negative strand rna viruses; rna binding, oligomerisation and binding to polymerase co-factor. | commentary on tawar, r.g.; duquerroy, s.; vonrhein, c.; varela, p.f.; damier-piolle, l.; castagné, n.; maclellan, k.; bedouelle, h.; bricogne, g.; bhella, d.; eléouët, j.-f.; rey, f.a. crystal structure of a nucleocapsid-like nucleoprotein-rna complex of respiratory syncytial virus. science2009, 326, 1279-1283. | 2010 | 21994598 |
| protein kinase r is a novel mediator of cd40 signaling and plays a critical role in modulating immunoglobulin expression during respiratory syncytial virus infection. | effective immunoglobulin responses play a vital role in protection against most pathogens. however, the molecular mediators and mechanisms responsible for signaling and selective expression of immunoglobulin types remain to be elucidated. previous studies in our laboratory have demonstrated that protein kinase r (pkr) plays a crucial role in ige responses to double-stranded rna (dsrna) in vitro. in this study, we show that pkr plays a critical role in igg expression both in vivo and in vitro. pk ... | 2011 | 21994357 |
| Communicable diseases prioritized for surveillance and epidemiological research: results of a standardized prioritization procedure in Germany, 2011. | To establish strategic priorities for the German national public health institute (RKI) and guide the institute's mid-term strategic decisions, we prioritized infectious pathogens in accordance with their importance for national surveillance and epidemiological research. | 2011 | 21991334 |
| respiratory syncytial virus vaccine development. | respiratory syncytial virus (rsv) is the leading cause of lower respiratory tract viral disease in infants and young children. presently, there are no explicit recommendations for rsv treatment apart from supportive care. the virus is therefore responsible for an estimated 160,000 deaths per year worldwide. despite half a century of dedicated research, there remains no licensed vaccine product. herein are described past and current efforts to harness innate and adaptive immune potentials to comb ... | 2011 | 21988307 |
| clinical impact of rt-pcr for pediatric acute respiratory infections: a controlled clinical trial. | real-time polymerase chain reaction (rt-pcr) testing is a quick sensitive method for detecting respiratory pathogens. we evaluated the diagnostic yield of rt-pcr assays and measured the effect of rapid reporting on patient care. | 2011 | 21987698 |
| comparison of differing cytopathic effects in human airway epithelium of parainfluenza virus 5 (w3a), parainfluenza virus type 3, and respiratory syncytial virus. | parainfluenza virus 5 (piv5) infects a wide range of animals including dogs, pigs, cats, and humans; however, its association with disease in humans remains controversial. in contrast to parainfluenza virus 3 (piv3) or respiratory syncytial virus (rsv), piv5 is remarkably non-cytopathic in monolayer cultures of immortalized epithelial cells. to compare the cytopathology produced by these viruses in a relevant human tissue, we infected an in vitro model of human ciliated airway epithelium and mea ... | 2011 | 21986028 |
| The post-infection outcomes of influenza and acute respiratory infection in patients above 50 years of age in Japan: an observational study. | Please cite this paper as: Ikematsu et al. (2011) The post-infection outcomes of influenza and acute respiratory infection in patients above 50 years of age in Japan: an observational study. Influenza and Other Respiratory Viruses DOI: 10.1111/j.1750-2659.2011.00296.x. Objectives Influenza can be a serious illness, especially for older people, and reducing the impact of influenza in elderly is important. The objective of this study was to estimate the prevalence and postinfection outcomes of in ... | 2011 | 21985038 |
| viral epidemiology of acute exacerbations of chronic obstructive pulmonary disease. | the role of viruses in acute exacerbations of chronic obstructive pulmonary disease (aecopd) needs further elucidation. the aim of the present study was to evaluate the molecular epidemiology of viral pathogens in aecopd. patients presenting to the emergency room with aecopd needing hospitalization were recruited. oropharyngeal and sputum samples were collected in order to perform microarrays-based viral testing for the detection of respiratory viruses. a total of 200 (100%) patients were analyz ... | 2011 | 21983132 |
| bug breakfast in the bulletin: respiratory syncytial virus. | 2011 | 21982261 | |
| effect of glucocorticoid in mice of asthma induced by ovalbumin sensitisation and rsv infection. | to investigate the inflammatory changes and the airway hyper-responsiveness in the asthma mouse model infected by respiratory syncytial virus and elucidate the relationship between the infection and the effect of glucocorticoid. | 2011 | 21980833 |
| immunoprotectivity of hla-a2 ctl peptides derived from respiratory syncytial virus fusion protein in hla-a2 transgenic mouse. | identification of hla-restricted cd8+ t cell epitopes is important to study rsv-induced immunity and illness. we algorithmically analyzed the sequence of the fusion protein (f) of respiratory syncytial virus (rsv) and generated synthetic peptides that can potentially bind to hla-a*0201. four out of the twenty-five 9-mer peptides tested: peptides 3 (f33-41), 13 (f214-222), 14 (f273-281), and 23 (f559-567), were found to bind to hla-a*0201 with moderate to high affinity and were capable of inducin ... | 2011 | 21980478 |
| Purification of human respiratory syncytial virus fusion glycoprotein. | Human respiratory syncytial virus (RSV) fusion glycoprotein (F) elicits neutralizing antibodies to RSV and has therefore attracted much attention as a suitable candidate antigen in the development of gene-based vaccines against RSV infections. However, a major obstacle in vaccine development has been the problem of antigen purification. To address this problem, we have developed a new method that combines sucrose gradient ultracentrifugation and a two-step chromatographic process, to purify RSV ... | 2012 | 21979254 |
| mortality burden of the a/h1n1 pandemic in mexico: a comparison of deaths and years of life lost to seasonal influenza. | the mortality burden of the 2009 a/h1n1 influenza pandemic remains controversial, in part because of delays in reporting of vital statistics that are traditionally used to measure influenza-related excess mortality. here, we compare excess mortality rates and years of life lost (yll) for pandemic and seasonal influenza in mexico and evaluate laboratory-confirmed death reports. | 2011 | 21976464 |
| [In Process Citation]. | 2011 | 21971620 | |
| Occult serious bacterial infection in infants younger than 60 to 90 days with bronchiolitis: a systematic review. | To summarize the risk of occult serious bacterial infection in the youngest febrile infants presenting with either clinical bronchiolitis or respiratory syncytial virus infection. | 2011 | 21969396 |
| The insertion of fluorescent proteins in a variable region of respiratory syncytial virus L polymerase results in fluorescent and functional enzymes but with reduced activities. | The respiratory syncytial virus (RSV) Large protein L is the catalytic subunit of the RNA-dependent RNA polymerase complex. Currently, no structural information is available for RSV L. Sequence alignments of L protein from human and bovine strains of RSV revealed the existence of two variable regions, VR1 and VR2. Following comparison with morbillivirus and rhabdovirus L genes, VR2, which is located between domains V and VI, was chosen as an insertion site for sequences encoding the epitope tag ... | 2011 | 21966341 |
| antiviral drugs for viruses other than human immunodeficiency virus. | most viral diseases, with the exception of those caused by human immunodeficiency virus, are self-limited illnesses that do not require specific antiviral therapy. the currently available antiviral drugs target 3 main groups of viruses: herpes, hepatitis, and influenza viruses. with the exception of the antisense molecule fomivirsen, all antiherpes drugs inhibit viral replication by serving as competitive substrates for viral dna polymerase. drugs for the treatment of influenza inhibit the ion c ... | 2011 | 21964179 |
| radio telemetry devices to monitor breathing in non-sedated animals. | radio telemetry equipment has significantly improved over the last 10-15 years and is increasingly being used in research for monitoring a variety of physiological parameters in non-sedated animals. the aim of this review is to provide an update on the current state of development of radio telemetry for recording respiration. our literature review found only rare reports of respiratory studies via radio telemetry. much of this article will hence report our experience with our custom-built radio ... | 2011 | 21964163 |
| il-17 boosts proinflammatory outcome of antiviral response in human cells. | excessive inflammation during bacterial and viral infections is destructive to the host and involves elevated production of proinflammatory cytokines. it is especially deleterious in organs with space constraints such as lung and the cns. indeed, a number of viruses that infect lungs, such as avian influenza virus, sars-associated coronavirus, and respiratory syncytial virus, elicit a very high level of proinflammatory cytokines; however, it is unclear what triggers their production. in this stu ... | 2011 | 21964025 |
| progress in understanding and controlling respiratory syncytial virus: still crazy after all these years. | human respiratory syncytial virus (rsv) is a ubiquitous pathogen that infects everyone worldwide early in life and is a leading cause of severe lower respiratory tract disease in the pediatric population as well as in the elderly and in profoundly immunosuppressed individuals. rsv is an enveloped, nonsegmented negative-sense rna virus that is classified in family paramyxoviridae and is one of its more complex members. although the replicative cycle of rsv follows the general pattern of the param ... | 2011 | 21963675 |
| Evidence for a Causal Relationship Between Allergic Sensitization and Rhinovirus Wheezing in Early Life. | RATIONALE: Both aeroallergen sensitization and virus-induced wheezing are risk factors for asthma development during early childhood, but the temporal developmental sequence between them is incompletely understood. OBJECTIVE: To define the developmental relationship between aeroallergen sensitization and virus-induced wheezing. METHODS: A total of 285 children at high-risk for allergic disease and asthma were followed prospectively from birth. The timing and etiology of viral respiratory wh ... | 2011 | 21960534 |
| Cost-effectiveness of Palivizumab for Respiratory Syncytial Virus Infection in High-risk Children, Based on Long-term Epidemiologic Data From Austria. | : To assess the cost-effectiveness of palivizumab, a monoclonal antibody against respiratory syncytial virus (RSV), in infants at high risk for severe RSV lower respiratory tract infection, such as premature infants, infants with bronchopulmonary dysplasia, and those with congenital heart disease, based on long-term epidemiologic data from Austria. | 2011 | 21960187 |
| comparison of four rt-pcr assays for detection of bovine respiratory syncytial virus. | rt-pcr assays for detection of brsv, based on four different sets of primers were optimized and evaluated for their sensitivity and specificity. primers used in this study were specific for genes encoding three brsv proteins, nucleoprotein n and glycoproteins f and g. our results indicated that rt-pcr with primers b7:b8 for g protein was the most efficient in detecting brsv. starters b7:b8 reacted specifically only with brsv strains, no cross-reaction with other closely related viruses to brsv w ... | 2011 | 21957740 |
| Respiratory viruses in children with cystic fibrosis: viral detection and clinical findings. | Please cite this paper as: Burns et al. (2011) Respiratory viruses in children with cystic fibrosis: viral detection and clinical findings. Influenza and Other Respiratory Viruses DOI: 10.1111/j.1750-2659.2011.00292.x. Background Viral detection from different respiratory sample types in children with cystic fibrosis (CF) is facilitated by available molecular methods, but optimum sampling strategies have not been identified. In addition, associations between viral detection and respiratory symp ... | 2011 | 21955319 |
| human metapneumovirus inhibits ifn-β signaling by downregulating jak1 and tyk2 cellular levels. | human metapneumovirus (hmpv), a leading cause of respiratory tract infections in infants, inhibits type i interferon (ifn) signaling by an unidentified mechanism. in this study, we showed that infection of airway epithelial cells with hmpv decreased cellular level of janus tyrosine kinase (jak1) and tyrosine kinase 2 (tyk2), due to enhanced proteosomal degradation and reduced gene transcription. in addition, hmpv infection also reduced the surface expression of type i ifn receptor (ifnar). these ... | 2011 | 21949722 |
| activation of toll-like receptor 9 and production of epitope specific antibody by liposome-encapsulated cpg-dna. | several investigators have shown that cpg-dna has outstanding effects as a th1-responsive adjuvant and that its potent adjuvant effects are enhanced by encapsulation with a liposome of proper composition. in this study, we showed that encapsulation with phosphatidyl-β-oleoyl-γ-palmitoyl ethanolamine (dope): cholesterol hemisuccinate (chems) complex enhances the immunostimulatory activity of cpg dna and the binding of cpg-dna to tlr9. we also examined involvement of myeloid differentiation protei ... | 2011 | 21944255 |
| Nanobodies®: new ammunition to battle viruses. | In 1989, a new type of antibody was identified, first in the sera of dromedaries and later also in all other species of the Camelidae family. These antibodies do not contain a light chain and also lack the first constant heavy domain. Today it is still unclear what the evolutionary advantage of such heavy chain-only antibodies could be. In sharp contrast, the broad applicability of the isolated variable antigen-binding domains (VHH) was rapidly recognized, especially for the development of thera ... | 2011 | 21939690 |
| relationship between lower respiratory tract infections caused by respiratory syncytial virus and subsequent development of asthma in japanese children. | several studies in western countries have found that lower respiratory tract infections (lrtis) caused by the respiratory syncytial virus (rsv) in infancy may subsequently trigger the development of asthma. in this study, we enrolled 262 infants under the age of 3 who had been admitted to our hospital with lrti between september 2002 and august 2003. rsv infection was diagnosed in these patients using an rsv rapid diagnostic kit and by measuring antibody titers in paired serum samples. in march ... | 2011 | 21937828 |