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combination pharmacotherapy with delayed countershock vs standard advanced cardiac life support after prolonged ventricular fibrillation.to test the hypothesis that combination pharmacotherapy with delayed countershock would produce higher rates of return of spontaneous circulation (rosc) and one-hour survival when compared with standard advanced cardiac life support (acls) therapy.200010634280
perinatal ontogeny of brain growth in the domestic pig.the perinatal development of the brain is highlighted by a growth spurt whose timing varies among species. the growth of the porcine cerebrum was investigated from the third trimester of gestation (70 days postconception) through the first 3.5 weeks of postnatal life (140 days postconception). the shape of the growth curves for cerebrum weight, total protein mass, total cell number (estimated by dna content), and myelination (estimated by cholesterol accretion) were described. the growth velocit ...200010632968
teaching and evaluation of basic surgical techniques: the university of british columbia experience.surgical technical education has traditionally followed an apprenticeship format. the need for innovative undergraduate programs using dry and wet labs prior to clinical exposure continues to be an area of debate. specific programs have been described to improve surgical skills; however, an accepted platform for training and evaluation of surgical skills programs has not been recognized. therefore, introduction of specific programs to teach undergraduate medical students surgical skills is essen ...199910630398
comparison of intravenous and endotracheal epinephrine during cardiopulmonary resuscitation in newborn piglets.to compare the efficacy of intravenous and endotracheal epinephrine administration, and intravenous administration above and below the diaphragm, during cardiopulmonary resuscitation in newborn piglets.199910628621
is it useful to measure supratentorial icp in the presence of a posterior fossa lesion? absence of transtentorial pressure gradients in an animal model.previous studies with animal models of supratentorial icp elevation have demonstrated a pressure gradient between the supratentorial and the infratentorial compartments. the present study was designed to investigate the possible presence of such a gradient in the case of infratentorial icp elevation. an inflatable infratentorial balloon catheter was implanted in seven domestic pigs. the infratentorial icp (icpi) was measured in the left cerebellar hemisphere, and the supratentorial icp (icps) wa ...199910627774
trichinella papuae n.sp. (nematoda), a new non-encapsulated species from domestic and sylvatic swine of papua new guinea.encapsulated and non-encapsulated species of the genus trichinella are widespread in sylvatic animals in almost all zoogeographical regions. in sylvatic animals from tasmania (australian region), only the non-encapsulated species trichinella pseudospiralis has been reported. between 1988 and 1998, non-encapsulated larvae of trichinella were detected in five domestic pigs and six wild boars from a remote area of papua new guinea. morphological, biological, and molecular studies carried out on one ...199910616929
comparison of carbon dioxide laser, erbium:yag laser, dermabrasion, and dermatome: a study of thermal damage, wound contraction, and wound healing in a live pig model: implications for skin resurfacing.advances in laser technology allow for precise tissue removal and minimal thermal damage. however, mechanisms for cosmetic improvement have not been determined. investigators have suggested that ablation, collagen shrinkage, and new collagen deposition all contribute to the clinical outcome.200010607327
effect of challenge dose and route on porcine reproductive and respiratory syndrome virus (prrsv) infection in young swine.porcine reproductive and respiratory syndrome virus (prrsv) is perceived to be highly infectious because of the rapid spread of the virus through populations of domestic swine throughout the world. however, no information has been published on the minimum infectious dose of prrsv and the effect of challenge dose on clinical response. in this experiment, ten groups of pigs (n = 3 per group) were inoculated with one of five different quantities (10(1)-10(5) fluorescent foci units per millilitre) o ...199910596410
a pilot study of in vivo immediate tissue contraction with co2 skin laser resurfacing in a live farm pig.it has been suggested that tissue ablation, collagen shrinkage, and new collagen deposition contribute to the clinical outcome of laser skin resurfacing.199910594596
survey of trichinella spp. infection in pigs from commercial piggeries in zimbabwe.trichinella spp. infection has never been reported among domestic pigs in zimbabwe. given that trichinella spp. occurs in a sylvatic cycle in zimbabwe and particularly in the light of the recent notification of the presence of the infection in farmed crocodiles, a survey was undertaken to evaluate the present epidemiological situation on commercial pig farms. carcasses of 7,446 fattener pigs (average 11.7% [+/- 0.2%] of those slaughtered from each farm) were investigated by artificial digestion ...199910588020
pulmonary dynamics of radiolabelled erythrocytes and leucocytes in early gram-negative sepsis in pigs.to study the pulmonary dynamics of erythrocytes and leucocytes in vivo in early experimental sepsis.199910574108
an african swine fever virus orf with similarity to c-type lectins is non-essential for growth in swine macrophages in vitro and for virus virulence in domestic swine.an african swine fever virus (asfv) orf, 8cr, with similarity to the c-type lectin family of adhesion proteins has been described in the pathogenic isolate malawi lil-20/1. the similarity of 8cr to cellular and poxvirus genes associated with cell adhesion, cell recognition and virus infectivity suggested that 8cr may be of significance to asfv-host cell interactions. sequence analysis of the 8cr orf from additional pathogenic asfv isolates demonstrated conservation among isolates from both pig a ...199910573162
laparoscopic v laparoscopy-assisted donor nephrectomy in the porcine model.laparoscopic donor nephrectomy is an established procedure in the porcine model. we sought to compare intraoperative variables between live laparoscopic (lap) and laparoscopy-assisted (lap-a) donor nephrectomy.199910569526
characterization of the dna polymerase loci of the novel porcine lymphotropic herpesviruses 1 and 2 in domestic and feral pigs.two novel porcine gammaherpesviruses, porcine lymphotropic herpesviruses 1 and 2 (plhv-1 and -2), have been detected by amplification of short dna polymerase (dpol) sequences from blood and spleen of domestic pigs while searching for unknown herpesviruses in pigs as possible risk factors in xenotransplantation. in the present study, the dpol genes of the two viruses and the open reading frames (orfs) that follow in the downstream direction were amplified by pcr-based genome walking from adaptor- ...199910567652
interventions to improve cardiopulmonary hemodynamics during laparoscopy in a porcine sepsis model.laparoscopy is increasingly used in severely ill and acutely septic patients. in animals undergoing laparoscopy, the hemodynamic response to sepsis is blunted. specific interventions to augment the hemodynamic potential may make laparoscopic intervention a safer alternative in septic patients. we compared different interventions to improve hemodynamic performance during exploratory laparoscopy in a porcine endotoxic shock model.199910549733
characterization of t1 relaxation and blood-myocardial contrast enhancement of nc100150 injection in cardiac mri.a new ultrasmall superparamagnetic iron oxide (clariscan; nc100150 injection) was studied in domestic farm pigs. the t1 effects were characterized for blood and myocardium and the blood-myocardial contrast was measured in t1-weighted cine images. the contrast-to-noise ratio (cnr) and signal-to-noise ratio (snr) were measured at baseline and contrast doses of 1 and 5 mg fe/kg body weight (bw) at end diastole and late systole. the t1 values for blood and myocardium were reduced by 97 and 43%, resp ...199910548789
genetic diversity of chinese native pigs inferred from protein electrophoresis.we examined protein polymorphism of 20 native pig breeds in china and 3 introduced pig breeds. thirty loci have been investigated, among which six loci were found to be polymorphic. especially, the polymorphism of malate dehydrogenase (mdh), adenylate kinase (ak), and two new alleles of adenosine deaminase (ada) had not been reported in domestic pigs and wild pigs. the percentage of polymorphic loci (p), the mean heterozygosity (h), and the mean number of alleles (a) are 0.200, 0.065, and 1.300, ...199910544802
functional remodelling and left ventricular dysfunction after repeated ischaemic episodes. a chronic experimental porcine model.this experimental study was set up to investigate left ventricular function and remodelling after repeated ischaemic episodes using magnetic resonance imaging (mri). a significant reduction in mortality due to coronary heart disease (chd) has been explained by both a decline in the incidence of acute myocardial infarction (ami) and an improved post-ami survival rate, suggesting a change in the natural history of chd. experimental intracoronary microembolization can induce different ischaemic pat ...199910540914
video-assisted thoracic diskectomy and anterior release: a biomechanical analysis of an endoscopic technique.this study evaluates the residual biomechanical stability of the spine following multilevel anterior diskectomies and anterior longitudinal ligament release using video-assisted thoracoscopic surgery (vats). eighteen domestic pigs were randomly divided into three groups of six pigs. group 1 underwent thoracic anterior release from t4-t9 using a left-sided vats approach, group 2 underwent thoracic anterior release from t4-t9 via a traditional left thoracotomy (open), and group 3 did not undergo s ...199910535554
in vivo renal vascular and tubular function in experimental hypercholesterolemia.hypercholesterolemia (hc) is often associated with impaired peripheral and coronary vascular responses to endothelium-dependent vasodilators, which are probably due to low bioavailability of nitric oxide. to examine the effect of hc on renal vascular and tubular function, 22 domestic pigs were studied after being fed a 12-week normal (n=11) or hc (n=11) diet. renal regional perfusion and intratubular contrast media concentration in each nephron segment (representing fluid reabsorption) were quan ...199910523374
anterior shear of spinal motion segments. kinematics, kinetics, and resultant injuries observed in a porcine model.a basic study of 56 porcine specimens in anterior shear loading.199910515011
identification of bloodmeals in haematophagous diptera by cytochrome b heteroduplex analysis.we developed a dna assay for bloodmeal identification in haematophagous insects. specific host cytochrome b gene sequences were amplified by pcr and classified on the basis of their mobility in a heteroduplex assay. in the blackfly simulium damnosum s.l. (diptera: simuliidae), human cytochrome b dna sequences were identifiable up to 3 days following ingestion of the bloodmeal. in the tsetse glossina palpalis (diptera: glossinidae) collected from tsetse traps in ivory coast, bloodmeals were ident ...199910514054
up-regulation of vascular endothelial growth factor mrna and angiogenesis after transmyocardial laser revascularization.angiogenesis has been proposed as a potential mechanism whereby transmyocardial laser revascularization (tmlr) has provided clinical relief of angina. experimental work has found histologic evidence supporting this, as well as an improved response when angiogenic growth factors have been added to tmlr. the purpose of this study was to demonstrate that the molecular response to tmlr was an increase in the production of endogenous vascular endothelial growth factor to promote angiogenesis.199910509969
spontaneous acceptance or rejection of orthotopic liver transplants in outbred and partially inbred miniature swine.results of clinical liver transplantation have shown that rejection and loss of human liver allografts occurs despite immunosuppression. because genetic disparity and liver immunogenicity remain a matter of controversy, we reexamined the fate of outbred liver allografts without immunosuppression and used partially inbred miniature swine, in which the genetics of major histocompatibility complex (mhc) antigens have been characterized and can be controlled.199910507476
combination anesthesia with ketamine and pentobarbital: a long-term porcine model.anesthesia of the pig poses great problems for experimental animal-based research and particularly in shock research. in this study, five mechanically ventilated domestic pigs were given long-term anesthesia with a combination of ketamine plus pentobarbital. circulatory parameters were recorded every 2 h via an arterial catheter placed in the right common carotid artery, a swan-gans thermodilution catheter (7f), that was placed in the pulmonary artery of the right middle-lobe in a wedge position ...199910494673
evaluation of biphasic transthoracic defibrillation in an animal model of prolonged ventricular fibrillation.to determine whether a biphasic defibrillation waveform (bdw) would produce a superior rate of converting prolonged ventricular fibrillation (vf) into a perfusing rhythm and delay the occurrence of asystole and/or pulseless electrical activity (pea) during the resuscitation attempt, when compared with a monophasic defibrillation waveform (mdw).199910490248
vasopressin versus epinephrine during cardiopulmonary resuscitation: a randomized swine outcome study.in animal models, vasopressin improves short-term outcome after cardiopulmonary resuscitation (cpr) for ventricular fibrillation compared to placebo, and improves myocardial and cerebral hemodynamics during cpr compared to epinephrine. this study was designed to test the hypothesis that vasopressin would improve 24-h neurologically intact survival compared to epinephrine. after a 2-min untreated ventricular fibrillation interval followed by 6 min of simulated bystander cpr, 35 domestic swine (we ...199910488942
[the wild boar of egypt].the wild boar, sus scrofa, is not a typical member of the egyptian wild fauna, although it appears to have lived in the nile delta and other suitable regions in the north of the country. however, historic populations were probably of mixed origin, including feral domestic pigs. it is incorrect, as is sometimes still done, to include the wild boar in the iconographic bestiary of ancient egypt and assume that the domestic pigs of ancient egypt derive from local wild boars.199910488431
the effects of biphasic and conventional monophasic defibrillation on postresuscitation myocardial function.the purpose of this study was to compare the effects of biphasic defibrillation waveforms and conventional monophasic defibrillation waveforms on the success of initial defibrillation, postresuscitation myocardial function and duration of survival after prolonged ventricular fibrillation (vf).199910483965
parasitic helminths of the pig: factors influencing transmission and infection levels.the occurrence of parasitic helminth species as well as infection intensities are markedly influenced by the type of swine production system used. the present review focusses mainly on the situation in temperate climate regions. generally, over the past decades there has been a decrease in the number of worm species and worm loads in domestic pigs due to a gradual change from traditional to modern, intensive production systems. the reasons for some species being apparently more influenced by man ...199910480725
health status of a recently discovered population of feral swine in kansas.twenty feral hogs (sus scrofa) from a newly discovered population on fort riley army base (kansas, usa) were shot and examined from november 1993 through february 1994 to assess the health of the population. the hogs were generally healthy, although serologic evidence indicated that some individuals had been exposed to parvovirus, enterovirus, and swine influenza. we found no indications of brucellosis, pseudorabies, or porcine respiratory and reproductive syndrome. lung worms (metastrongylus sp ...199910479106
[wild is not really wild: brain weight of wild domestic mammals].domestication leads to the reduction of brain weight, decreases reach from 8.1% in laboratory rats up to 33.6% in domesticated pigs. the question is: do brain weights increase by feralization? we compared the brain weights of domesticated mammals (cat, dog, pig, goat, ass) with their feral forms. in none of the cases studied, brain weight is increased in wild domestic mammals. so, feral mammals do not return back to the status of their wild species.199910472721
intraosseous vasopressin improves coronary perfusion pressure rapidly during cardiopulmonary resuscitation in pigs.intravenous administration of vasopressin during cardiopulmonary resuscitation (cpr) may be more effective than optimal doses of epinephrine. the main purpose of this study was to determine whether intraosseous vasopressin achieves serum drug levels comparable with intravenous doses during cpr and, additionally, to evaluate the effects of intraosseous vasopressin during cpr.199910470765
classical swine fever in sardinia: epidemiology of recent outbreaks.a variable region of the gene encoding the major glycoprotein (e2) of classical swine fever virus (csfv) was sequenced from 12 sardinian isolates which had been obtained from three geographically distinct regions of the island. phylogenetic analysis of these viruses and others characterized in previous studies [1, 2] indicated that (a) the sardinian viruses were all members of the common european subgroup 2.3 and were clearly distinct from live vaccines recently used in this area; (b) they could ...199910459661
effects of an environmental endocrine disruptor on fetal development, estrogen receptor(alpha) and epidermal growth factor receptor expression in the porcine male genital tract.we studied the effect of a potent reproductive tract toxin, 2,3,7,8-tetrachlorodibenzo-rho-dioxin, on fetal development and expression of estrogen receptor alpha and epidermal growth factor receptor (egfr) in male swine.199910458397
[the pulmonary arteries and veins of miniature swine, together with a common view of their supportive and stabilizing functions in the lung].with respect to distribution and flow, the vasa pulmonalia resemble those of the normal grown domestic pig to a great extent. the present article investigates whether, like the human lung (hayek, 1970), the arrangement of the blood vessels has a function over and above that of directing blood; that is to say, a mechanical function. the arteries through which the blood streams act as flexible supporting structures, and the veins as elastic holding structures, for the quadrupede animal also.199910458018
effect of hyperoxia and hypoxia on subcutaneous tissue gases and ph. an experimental study in pigs.the effect of different fractions of inspired oxygen on subcutaneous oxygen tension, carbon dioxide tension and ph in relation to arterial and mixed venous blood gases and ph and hemodynamic variables was evaluated in 13 domestic pigs. subcutaneous, arterial and mixed venous oxygen tensions were closely correlated (r = 0.94). a minor significant increase in subcutaneous carbon dioxide tension was noted during hyperoxia (p < 0.05). a major increase (33%) was noted during the last step of hypoxia, ...199910449992
effects of dominance and familiarity on behaviour and plasma stress hormones in growing pigs during social confrontation.behavioural patterns and plasma adrenaline, noradrenaline and cortisol responses were studied in domestic pigs with different dominance status during 10-h social confrontation tests with a familiar and an unfamiliar group. eight trials were carried out, where in each trial two groups of nine growing pigs (12 weeks old) were randomly formed. the pigs with the highest (hr) and lowest (lr) rank from each group were selected as test animals, provided with surgically implanted catheters and kept in s ...199910445002
[experimental study of the architecture of skin following tension traction and wound closure].the experiment performed on domestic pig, was designed to investigate the architecture of the stretching skin and the effect of tension traction on the wound closure. an 7 cm x 3.5 cm was drawn at a standard position. drawing horizontal and vertical lines on the traction area, points of 1 cm apart were tattooed on the lines. a traction force measuring device was used to draw the wound edges together. measurements of the distances between the points were made. an 7 cm x 10 cm wound was made on id ...199810437064
resting rectal temperature of vietnamese potbellied pigs.to determine resting rectal temperatures of vietnamese potbellied pigs.199910434970
central haemodynamics in experimental acute pancreatitis.to investigate central haemodynamics in severe and mild acute pancreatitis in pigs.199910433147
trichinella nativa in sylvatic wild boars.of 17 trichinella isolates from domestic pigs and wild boars (sus scrofa) in regions where trichinella nativa is widespread among sylvatic animals, two wild boars from estonia were found to be naturally infected with this trichinella species. the other 15 animals were infected with trichinella spiralis. trichinella nativa is tolerant to freezing when in the muscles of carnivores. the biological characteristics and temperature tolerance of this species in swine need to be further investigated if ...199910431373
beneficial effects of leukocyte-depleted blood and low-potassium dextran solutions on microvascular permeability in preserved porcine lung.modified euro-collins (ec) solution, a crystalloid intracellular-type solution, has been commonly used for pulmonary preservation. several experimental studies have shown the advantages of using extracellular colloid-based solutions. the aim of this study was to compare the quality of preservation of two extracellular colloid solutions, leukocyte-depleted blood (bl) and low-potassium dextran (lpd) solutions, with that of ec solution. lungs of 22 domestic pigs were flushed and preserved with ec ( ...199910430747
decreased cerebrospinal fluid absorption during abdominal insufflation.intracranial pressure (icp) is known to rise during induced co(2) pneumoperitoneum. this rise correlates with an increase in inferior vena caval pressure; therefore, it is probably associated with increased pressure in the lumbar venous plexus. branches of this plexus communicate with arachnoid villi in the lumbar cistern and the dural sleeves of spinal nerve roots-areas where cerebrospinal fluid (csf) absorption to normally takes place. the increased venous pressure in this area may impede csf ...199910430688
the differentiation of traumatic and heat-related fractures in burned bone.interpretations of antemortem and perimortem trauma are complicated when dealing with cases involving extreme exposure to fire. this investigation attempts to discern the signatures of perimortem trauma from heat related trauma. femora of domestic pig, sus scrofa, with minimal soft tissue and articulated patellae were subjected to varying traumatic forces. skeletal elements were impacted with blunt and sharp forces, cut with varying instruments, subjected to torsional forces of shot. bones were ...199910408102
antifibrinolytic additives to fibrin glue for laparoscopic wound closure in urinary tract.fibrinolytic activity of urine may rapidly degrade fibrin glue used in the urinary tract, thereby limiting tissue adhesion. the goals of this study were to verify the ability of antifibrinolytic agents to delay the degradation of fibrin glue in the urinary tract and to assess the results of this delay on subsequent wound healing.199910405907
laparoscopic replacement of urinary tract segments using biodegradable materials in a large-animal model.we elected to evaluate laparoscopic segmental bladder and ureteral replacement with free biodegradable graft materials in a large-animal model.199910405899
laparoscopic renal cryoablation: acute and long-term clinical, radiographic, and pathologic effects in an animal model and application in a clinical trial.to evaluate renal cryosurgery by studying the feasibility of laparoscopic delivery and the radiographic characteristics and histopathologic effects in a porcine model using different freeze cycles. on the basis of the results, a clinical trial of laparoscopic cryosurgical ablation in select patients with clinical stage t1 renal tumors was started.199910405898
temporal differences in the responses of the pituitary adrenocortical axis, the sympathoadrenomedullar axis, heart rate, and behaviour to a daily repeated stressor in domestic pigs.in this study we examined responses of domestic pigs (sus scrofa domestica) to a daily repeated stressor. in particular, we focused on differences in temporal changes across treatment repetition between the pituitary adrenocortical axis, the sympathoadrenomedullar axis, heart rate, and behaviour. to induce a stress response, we separated eight castrated male pigs from their group mates visually and auditorily for 1 h on 10 successive days. overall, enhanced plasma levels of cortisol, acth, and a ...199910405105
statistical method for characterization of hypertrophic cardiomyopathy by use of morphologic and pathologic measurements in pigs (sus scrofa domestica).hypertrophic cardiomyopathy (hcm) is characterized by symmetric or asymmetric hypertrophy of the left and/or right ventricle. morphologic and pathologic indices (mi and pi) of hearts were established for classification of hcm in pigs.199910403442
responses of single taste fibers and whole chorda tympani and glossopharyngeal nerve in the domestic pig, sus scrofa.whole nerve, as well as single fiber, responses in the chorda tympani proper (ct) and glossopharyngeal (ng) nerves of 1- to 7-week-old pigs were recorded during taste stimulation. in the ct acids and in the ng bitter compounds gave the largest responses. both nerves exhibited large responses to monosodium glutamate (msg), msg with guanosine 5'-monophosphate (gmp) and msg with inositine 5'-monophosphate (imp) as well as to glycine, xylitol, sucrose, fructose and glucose. alitame, aspartame, betai ...199910400449
morphological study of mast cell localization in the wall of the proximal tubule in the domestic swine kidney.morphological studies to localize mast cells in the wall of the convoluted and straight parts of the proximal tubules of the domestic swine kidney were performed. kidney perfusion was carried out in 8-month-old pigs and semi-thin and ultrathin sections were prepared after a routine treatment. a light microscopic study showed that mast cells were not frequently found. most were localized in the wall of the proximal convoluted tubule, while in the proximal straight tubule wall they were fewer in n ...199910386001
characterization of porcine and ovine oesophagostomum spp. by isoenzymatic patterns and restriction-fragment-length polymorphisms (rflps).four different morphological and biometrical populations of oesophagostomum have been identified, using classical taxonomy methods, from sus scrofa domestica (pigs): o. dentatum (od), o. quadrispinulatum (oq), o. granatensis (og), and a fourth population, including individuals with morphological and biometric parameters overlapping these three species that were clasified as oesophagostomum sp. the g6pd and mdh isoenzymatic patterns did not discriminate between the three species, while gpi showed ...199910379817
african swine fever virus: a b cell-mitogenic virus in vivo and in vitro.the two major characteristics of pathogenesis in african swine fever virus (asfv) infections of domestic pigs are massive b-cell apoptosis and haemorrhage. the effects of asfv on porcine b cells have therefore been systematically examined in vivo, by using virus-infected pigs and scid-beige mice reconstituted with porcine bone marrow, and in vitro, by using porcine b-cell lines and b cells from normal and asfv-infected pigs. secretion of porcine ig was stimulated by asfv both in vivo and in bone ...199910374963
[linkage of a porcine esterase d locus with a lethal factor].this work is devoted to a linkage study of the gene controlling esterase d and a lethal factor in domestic pig. segregation for two alleles esdb, which originated from populations of miniature swine and pigs of a large white breed, and the effect of these variants on the number of offspring in a litter were compared. the results showed a linkage of locus esd with a lethal factor. this lethal factor causes the mortality of homozygotes and half of the heterozygotes in a litter, which depends on th ...199910368786
detection of glycosidic residues in carpal glands of wild and domestic pig revealed by basic and lectin histochemistry.carpal glands are compound tubuloalveolar glands, located on the medial surface of the carpus. this study was carried out on samples from carpal glands of adult wild and domestic pigs of both sexes. we elucidated the glycosidic composition of carpal gland secretion in situ using traditional histochemical methods and lectin histochemistry. some secretory cells exhibited an intense reaction with pas in both wild and domestic pigs. lectin histochemistry showed differences in the localization and co ...199910363109
ct-monitored percutaneous cryoablation in a pig liver model: pilot study.to determine the safety and feasibility of percutaneous cryoablation with computed tomographic (ct) guidance in a pig liver model.199910352592
electrocardiographic prediction of the success of cardiac resuscitation.to identify a method for predicting the success or failure of a defibrillatory shock such as to avoid potentially detrimental interruptions of cardiopulmonary resuscitation (cpr). such a method would also guide more optimal programming of automated external defibrillators.199910321659
prevalence of toxoplasma gondii infection in domestic pigs in the new england states.to determine regional prevalence of infection with toxoplasma gondii, 1897 pigs from 85 farms in five new england states were tested using a modified direct agglutination test. sera were diluted 1:25 and a titer at this dilution was indicative of t. gondii infection. farm management questionnaires were completed at the time of blood collection and were used to develop descriptive statistics on farms tested and to determine measures of association for risk factors for the presence of t. gondii-se ...199910321584
evaluation of ureteric contraction: a comparison among ring, spiral-cut and longitudinal segments.to determine the optimal contractile response of isolated ureters to inflammatory mediators and neurotransmitters by evaluating four common methods of ureteric suspension.199910233460
mr-guided percutaneous nephrostomy of the nondilated upper urinary tract in a porcine model.the purpose of this study was to assess the safety and feasibility of mr-guided percutaneous nephrostomy of the nondilated contrast-enhanced upper urinary tract in a porcine model.199910227492
prevalence and risk association for trichinella infection in domestic pigs in the northeastern united states.to determine trichinella infection in a selected group of farm raised pigs, 4078 pigs from 156 farms in new england and new jersey, employing various management styles, were selected based on feed type (grain, regulated waste, non-regulated waste). the number of pigs bled from each farm were based on detecting infection assuming a 0.05 prevalence rate. serum was tested by enzyme-linked immunoassay for antibodies to trichinella spiralis. seropositive pigs were tested by digestion at slaughter (wh ...199910223350
effect of porcine reproductive and respiratory syndrome virus (prrsv) on alveolar lung macrophage survival and function.porcine reproductive and respiratory syndrome virus (prrsv) recently emerged as an important cause of reproductive disorders and pneumonia in domestic pigs throughout the world. acute cytocidal replication of prrsv in alveolar lung macrophages causes the acute pneumonia; however, it remains largely unresolved whether there may also be a predisposition to longer-term local immunodeficiency in the prrsv-convalescent lung. we applied various flow cytometric techniques to study the interplay between ...199910223319
cardiopulmonary effects of the laparoscopic pneumoperitoneum in a porcine model of adult respiratory distress syndrome.increasingly, laparoscopy is being used in critically ill patients in whom there is a question of intra-abdominal sepsis. we examine the cardiopulmonary effects of laparoscopy in a porcine model of adult respiratory distress syndrome (ards).199910219857
prevalence of toxoplasma gondii antibodies in sera of domestic pigs and some wild game species from zimbabwe.serum samples of domestic pigs (sus scrofa), elands (taurotragus oryx), sable antelopes (hippotragus niger), warthogs (phacochoerus aethiopicus), bushpigs (koiropotamus [potamochoerus] koiropotamus), white rhinos (ceratotherium simus), african buffalos (syncerus caffer), wildebeest (connochaetas taurinus), and african elephants (loxodonta africana) from zimbabwe were tested for toxoplasma gondii igg antibodies by the modified agglutination test (mat) with whole formalized tachyzoites and mercapt ...199910219323
does desmopressin reduce bleeding in liver surgery? an experimental study on pigs.the main problem with liver surgery is to control intraoperative bleeding. bleeding together with postoperative infection and liver failure are the most common causes of postoperative morbidity and mortality in liver surgery. hospitals with experience of liver resection, report postoperative mortality rates of 3.2%-14.3%. improved results in liver surgery have been made by refinement of surgical methods and modern techniques. desmopressin acetate (octostim) reduces bleeding time by activating pl ...199910218138
vasopressin improves vital organ blood flow after prolonged cardiac arrest with postcountershock pulseless electrical activity in pigs.although a benefit of vasopressin when compared with epinephrine was shown during cardiopulmonary resuscitation (cpr) after a short duration of ventricular fibrillation cardiac arrest, the effect of vasopressin during prolonged cardiac arrest with pulseless electrical activity is currently unknown.199910199526
vascularization and cartilage mineralization of the thyroid cartilage of munich minipigs and domestic pigs.thyroid cartilages of munich minipigs and domestic pigs were investigated by polychrome sequential labeling, radiography, intravascular injections, histologic examination and scanning electron microscopy in order to gain further insight into the process of vascularization and cartilage mineralization. the relationship between vascularization and cartilage mineralization has only been studied in chondroepiphyses of long bones. vessels branch off the perichondrial vascular network and enter parts ...199910195303
antilithiasic effect of beta-cyclodextrin in lpn hamster: comparison with cholestyramine.beta-cyclodextrin (bcd), a cyclic oligosaccharide that binds cholesterol and bile acids in vitro, has been previously shown to be an effective plasma cholesterol lowering agent in hamsters and domestic pigs. this study examined the effects of bcd as compared with cholestyramine on cholesterol and bile acid metabolism in the lpn hamster model model for cholesterol gallstones. the incidence of cholesterol gallstones was 65% in lpn hamsters fed the lithogenic diet, but decreased linearly with incre ...199910191297
[comparison of laboratory diagnostic methods for the detection of infection with the virus of classical swine fever in the early inspection phase: an experimental study].virus isolation in the pk-15 cell culture, two commercial antigen elisas, reverse transcriptional-polymerase chain reaction (rt-pcr), and flow cytometry have been evaluated to detect viremic pigs in the early period of classical swine fever virus (csfv) infection. domestic pigs were experimentally inoculated with the virulent csfv strain 'alfort 187' and two field isolates. csfv isolation and rt-pcr were found to be the most sensitive methods for the detection of highly virulent csfv in the earl ...199910189722
melatonin receptors in neonatal pig brain and pituitary gland.summer infertility remains a major problem in domestic pigs. it has been proposed that sows which display this trait are inherent seasonal breeders. the influence of photoperiod on domestic pigs has been difficult to ascertain as significant diurnal fluctuations in blood levels of the pineal hormone, melatonin, which provide a direct neuroendocrine transduction of the ambient photoperiod in other species, remain questionable in adult pigs. to investigate whether the pig is potentially receptive ...199910102759
effects of overlap and pass number in co2 laser skin resurfacing: a study of residual thermal damage, cell death, and wound healing.newer co2 laser systems incorporating short pulse and scanning technology have been used effectively to resurface the skin. as the number of resurfacing cases has increased, hypertrophic scarring has been reported more commonly. previous dermabrasion and continuous wave co2 studies have suggested that depth of injury and thermal damage are important predictors of scarring for a given anatomic region. to determine whether rapid overlapping of laser pulses/scans significantly altered wound healing ...199910100647
confirmed quantitative trait loci for fatness and growth on pig chromosome 4.quantitative trait loci (qtls) with large effects on fatness and growth have been identified previously on pig chromosome 4 in an intercross between the european wild pig and large white domestic pigs. two f2 sows, heterozygous for the actual chromosome region, were backcrossed to a large white boar, and two backcross (bc1) boars were in turn backcrossed to large white/landrace sows. one of the boars was heterozygous for an intact wild pig-derived qtl region, whereas the other carried a recombin ...199910098263
[effects of octreotide (somatostatin analog sms 201-995) on superior mesenteric artery blood flow in swine. an experimental study using doppler color ultrasonography].the effect of octreotide on splanchnic haemodynamics was investigated in 25 domestic pigs by mean of us-colordoppler. in a blind-operator design the superior mesenteric artery (sma) blood flow was evaluated before and after i.m. injection of 5, 10, 20, 25 micrograms/kg or of an equivalent volume of saline solution. seven serial measurements were taken every 10 min in each pig excluding the higher and the lower values. the administration of 5 and 10 micrograms/kg determined a rapid and stable (3 ...199910097449
genetic evidence for the role of gdp-mannose in plant ascorbic acid (vitamin c) biosynthesis.vitamin c (l-ascorbic acid; asa) acts as a potent antioxidant and cellular reductant in plants and animals. asa has long been known to have many critical physiological roles in plants, yet its biosynthesis is only currently being defined. a pathway for asa biosynthesis that features gdp-mannose and l-galactose has recently been proposed for plants. we have isolated a collection of asa-deficient mutants of arabidopsis thaliana that are valuable tools for testing of an asa biosynthetic pathway. th ...199910097187
vascular endothelial growth factor in porcine coronary arteries following balloon angioplasty.the present study was a preliminary inquiry into the presence of vascular endothelial growth factor (vegf) in a model of coronary artery response to injury. we examined domestic pigs who had received a diet enriched in saturated fat and cholesterol and undergone balloon angioplasty of one or more coronary arteries. immunohistochemical analysis of the coronary arteries 2 months after injury revealed the presence of vegf distributed throughout the media and neointima of the angioplasty lesions and ...199910084675
plesiomonas shigelloides and its serovars in animals in the czech republic--region moravia.in the period of 1994-1997, 4,552 samples of sectional, clinical material and environment were examined for the presence of plesiomonas shigelloides. in 55 cases (1,21%) plesiomonas shigelloides was isolated. within 55 isolated strains, 26 serovars have been successfully identified. among other secured cases was plesiomonas shigelloides isolated in pig (sus scrofa domestica), mouse mus musculus), binturong (arctictis binturong) and raccoon (pocyon lotor). in mandarin duck (aix galericulata) the ...199910084023
erv-l elements: a family of endogenous retrovirus-like elements active throughout the evolution of mammals.we have previously identified in the human genome a family of 200 endogenous retrovirus-like elements, the herv-l elements, disclosing similarities with the foamy retroviruses and which might be the evolutionary intermediate between classical intracellular retrotransposons and infectious retroviruses. southern blot analysis of a large series of mammalian genomic dnas shows that herv-l-related elements-so-called erv-l-are present among all placental mammals, suggesting that erv-l elements were al ...199910074184
sutureless small bowel anastomoses: experimental study in pigs.to evaluate a new technique for experimental anastomosis with fibrin glue, and to compare the results with those of stapled and one-layer sutured anastomosis.199910069636
hepatic cryosurgery via minilaparotomy in a porcine model: an alternative to open cryosurgery.cryosurgery of liver lesions is becoming increasingly accepted for the ablation of liver tumors. attempts at laparoscopic cryosurgery have been very limited and often need to be converted to open laparotomy due to the complexity of the procedure.199910064758
oral immunisation of swine with a classical swine fever vaccine (chinese strain) and transmission studies in rabbits and sheep.seven experiments including a total of 47 pigs, 11 wild boars, 26 rabbits, 10 hares and 16 sheep were carried out to assess the efficacy, safety and transmission of the chinese vaccine strain of the classical swine fever virus (csfv) administrated by the oral route. within 3 weeks after oral vaccination, a clear seroconversion occurred in the pigs. six weeks after vaccination, vaccinated pigs were fully protected against a virulent challenge. the c-strain was not isolated from tonsils, spleen, l ...199910063532
the effect of wiping on skin resurfacing in a pig model using a high energy pulsed co2 laser system.the impact of wiping in laser skin resurfacing has not been systematically studied.199910037508
filtered versus unfiltered technetium sulfur colloid in lymphatic mapping: a significant variable in a pig model.lymphatic mapping with sentinel node biopsy is becoming a standard diagnostic test for melanoma and is being extensively investigated for use with other soft tissue tumors. both filtered and unfiltered technetium sulfur colloid (tc 99) have been used for preoperative lymphoscintigraphy, as well as intraoperative lymphatic mapping, and it is not clear if one is preferable over the other. the purpose of this study was to compare these two preparations to determine whether the form of tc 99 used af ...199910030419
trichinellosis in the united states, 1991-1996: declining but not gone.since the u.s. public health service began recording statistics on trichinellosis in 1947, the number of cases reported by state health departments has decreased steadily. in the late 1940s, health departments reported an average of 400 cases and 10-15 deaths each year. from 1991 to 1996, the period covered in this report, three deaths in 230 cases were reported to the centers for disease control and prevention (an average of 38 cases per year), including 14 multiple case outbreaks from 31 state ...19999988325
adventitial angiogenesis early after coronary angioplasty : correlation with arterial remodeling.the spatial correlation between arterial wall microvessels and the accumulation of atherosclerotic plaque is well documented. the role of these microvessels in the development of primary and restenotic lesions is not known. to investigate the effect of interventional procedures on arterial wall microvessels, we studied the adventitial microvascularity of porcine coronary arteries subjected to angioplasty. twenty-two juvenile domestic swine were subjected to single or repeated (double) balloon an ...19999974402
seroprevalence of trichinella infection in domestic swine based on the national animal health monitoring system's 1990 and 1995 swine surveys.swine sera collected by the us department of agriculture's center for animal health monitoring during 1990 and 1995 was tested for antibodies to trichinella spiralis using an enzyme immunoassay. from a total of 3048 sera collected from lactating sows in 1990, five sera tested positive for a prevalence of 0.16%. from a total of 7987 sera collected from both finishing pigs and gestating sows in 1995, one serum was positive for a prevalence of 0.013%. responses to questionnaires administered at the ...19999950336
introducing fluorine-18 fluoromisonidazole positron emission tomography for the localisation and quantification of pig liver hypoxia.fluorine-18 labelled fluoromisonidazole ([18f]fmiso) has been shown to accumulate in hypoxic tissue in inverse proportion to tissue oxygenation. in order to evaluate the potential of [18f]fmiso as a possible positron emission tomography (pet) tracer for imaging of liver tissue hypoxia, we measured the [18f]fmiso uptake in 13 domestic pigs using dynamic pet scanning. hypoxia was induced by segmental arterial hepatic occlusion. during the experimental procedure the fractional concentration of insp ...19999933343
laparoscopic renal cryotherapy in swine: comparison of puncture cryotherapy preceded by arterial embolization and contact cryotherapy.variable results of open, percutaneous, and laparoscopic cryosurgery of the kidney have been reported. minimally invasive approaches to lower-pole renal ablation were prospectively compared in swine: laparoscopic puncture cryoablation preceded by arterial embolization, laparoscopic contact cryosurgery, and arterial embolization alone. eighteen kidneys in nine domestic pigs were randomized to one of three treatment groups of six organs each: (1) puncture cryotherapy preceded by selective lower-po ...19989895264
short report: identification of the likely etiologic agent of human trichinellosis in sicily (italy) between 1933 and 1946.larvae of trichinella sp. collected in 1961 from muscle samples of the last infected domestic pig slaughtered in sicily (italy), and preserved in absolute ethyl alcohol until 1997, have been identified as trichinella spiralis by the polymerase chain reaction. this finding explains the severity of the clinical picture, resulting in death, and the high number of fatal cases (20 of 184, 11%) observed in three sicilian outbreaks between 1933 and 1945, caused by the consumption of sausages from pigs ...19989886198
heterogeneity of myocardial edema in isolated pig hearts after perfusion with different types of cardioprotective solutions.the extent and distribution of myocardial edema induced by perfusion with cardioprotective solutions is of great interest. domestic pig hearts (n = 12) were perfused in situ after aortic cross clamping either with bretschneider's cardioplegic solution (htk, 4 degrees c, n = 3), with a heparinized krebs-henseleit solution containing 30 mmol/l 2,3 butanedionemonoxime (bdm, 4 degrees c, n = 3) or with heparinized pig blood (hpb, 24 degrees c, n = 3). after a three-hours storage period, magnetic res ...19989885120
facilitating intracorporeal colorectal anastomoses.an experimental study was undertaken to evaluate whether a previously described technique for laparoscopic sigmoid resection with intracorporeal resection-anastomosis and specimen removal via a suprapubic incision could be facilitated and applied to the rectum.19989876710
functional mr imaging of the porcine kidney: physiologic changes of prolonged pneumoperitoneum.increased intraabdominal pressure (ipa) during laparoscopy has been associated with decreased urine output. the purpose of this study was to use a noninvasive mri technique to measure renal vessel flow velocity and change in differential renal medulla and cortex perfusion during pneumoperitoneum.19979876643
characterization of reciprocal translocations in pigs using dual-colour chromosome painting and primed in situ dna labelling.we report the use of dual-colour chromosome painting to determine the exact nature of certain chromosome rearrangements observed in the pig (sus scrofa domestica). the chromosomal abnormalities were detected by gtg- and rbg-banding techniques. the initially proposed interpretations were: (1) rcp(6;13)(p1.5;q4.1); (2) rcp(11;16)(p1.4;q1.4); (3) rcp(6;16)(p1.1;q1.1); (4) rcp(13;17)(q4.1;q1.1); (5) rcp(6;14)(q2.7;q2.1); (6) rcp(3;5)(p1.3;q2.3); (7) rcp(2; 14)(q1.3;q2.7); (8) rcp(15;17)(q1.3;q2.1). ...19989872665
expression pattern of extracellular matrix proteins in the pancreas of various domestic pig breeds, the goettingen minipig and the wild boar.in spite of progress in biotechnology, isolation of porcine pancreatic islets remains a difficult task with unpredictable results. one reason could be the lack of knowledge as to the expression of extracellular matrix proteins in porcine exocrine and endocrine tissues, particularly in "islet capsules". such proteins are subject to digestion by proteases, yet they might have a protective function for the fragile islets.19979869860
[the function of nitric oxide in the necrosis of avulsed skin flap in domestic pig].to investigate the function of nitric oxide (no) and nitric oxide synthetase (nos) inhibitor, n-nitro-l-arginine methyl ester (l-name), the skin avulsion model was made in the lower extremity of pig. the methods of measurement of size of the survived flap, weighing, immunocytochemistry and hybridization in situ were employed, so that the survival surface area of flaps, tissue wet/dry weight ratio, no content in the serum, gene expression of no and nos content in the flap tissue were determined, ...19979867957
the soft tissue cover of the mandibular condyle. differentiation in histological forms and age-related changes of aggrecan- and versican-like proteoglycans.age-related changes of the composition of the extracellular matrix of the soft tissue cover of the mandibular condyle (stc), especially of the large proteoglycans, have been investigated. proteoglycans were extracted from the stc of neonatal, juvenile and adult domestic pigs, fractionated by density gradient centrifugation and analyzed by electrophoresis/western blotting. experiments revealed firstly that a large cs/ks proteoglycan (aggrecan) is an essential constituent of the stc at all ages. t ...19989857606
utility of the harmonic scalpel for laparoscopic partial nephrectomy.laparoscopic partial nephrectomy (lpn) remains a technically challenging procedure largely because of the lack of methods for obtaining consistent parenchymal hemostasis. the objective of this study was to determine if the extent of resection influences the ability of the harmonic scalpel to achieve hemostasis and to define the cases in which the harmonic scalpel is appropriate for lpn. thirty lpns were performed in a 25-kg domestic pig model. the blunt blade of the laparoscopic harmonic scalpel ...19989847067
ex vivo comparison of four lithotripters commonly used in the ureter: what does it take to perforate?we hoped to determine the number of pulses and energy needed to create acute ureteral perforations with four different lithotripters in a reproducible ex vivo model. a simple model was constructed to control variables in the testing such as wall thickness, intraluminal pressure, distance between the probe tip and ureter, and power delivered to tissue. segments of domestic pig ureter were prepared and fixed in position in a normal saline (ns) bath at room temperature. we then attempted perforatio ...19989847062
[practical implementation of control measures against highly contagious animal diseases in the territory of the european union exemplified by classical swine fever].the reasons for abandoning prophylactic vaccination against highly contagious animal diseases in the european union on principle are illustrated. the practical implementation of control measures under the current framework are explained with respect to an outbreak of classical swine fever, emphasizing the role of the local crisis center, the veterinary diagnostic laboratories and the need for an overall control and coordination of the campaign at the regional level. in view of the continuous ris ...19989818462
shigella infection as observed in the experimentally inoculated domestic pig, sus scrofa domestica.the domestic pig, sus scrofa domestica, was investigated as a potential animal model for shigellosis. we examined the effects of pig age, pig breed and antibiotic pretreatment upon shigella infection. shigella dysenteriae, and shigella flexneri (both virulent and avirulent strains) were utilized. our results indicated that young (4-week-old), conventionally re ared, domestic pigs were routinely, but briefly, colonized (average=3.5+/-2.5 days) following oral or gavage administration ofs. flexneri ...19989817822
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