Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [the significance of mitochondria control region (d-loop) in intraspecific genetic differentiation of fish]. | mtdna has been widely used as a molecular marker to investigate the phylogenesis of many species. d-loop has important value for investigating intraspecific genetic differentiation because of its high mutation accumulation. fish is the most primitive vertebrate, but by far is the most dominant in number. the distribution of fish is broad and its origin is very complicated, has always been a very interesting subject for investigation. d-loop has many important implications for investigating the i ... | 2006 | 16551607 |
| genetic differences in natural antibody levels in common carp (cyprinus carpio l.). | in mammals, natural antibodies (nabs) are mostly of the igm isotype and can bind to a particular antigen or pathogen even if the host has never been exposed. despite their early detection and abundance, the exact role and genetic control of nabs remain unclear. we have used an indirect elisa with three different antigens (keyhole limpet haemocyanin, chicken ovalbumin and bovine serum albumin) to demonstrate the ubiquitous presence of nabs in common carp. serum levels of nabs increased with age, ... | 2006 | 16545579 |
| cell cycle- and apoptosis-regulatory protein-1 is involved in apoptosis signaling by epidermal growth factor receptor. | carp-1, a novel apoptosis inducer, regulates apoptosis signaling by diverse agents, including adriamycin and growth factors. epidermal growth factor receptor (egfr)-related protein (errp), a pan-erbb inhibitor, inhibits egfr and stimulates apoptosis. treatments of cells with errp or iressa (an egfr tyrosine kinase inhibitor) results in elevated carp-1 levels, whereas antisense-dependent depletion of carp-1 causes inhibition of apoptosis by errp. carp-1 is a tyrosine-phosphorylated protein, and e ... | 2006 | 16543231 |
| plasticity of respiratory structures--adaptive remodeling of fish gills induced by ambient oxygen and temperature. | while a large surface area combined with short diffusion distances make fish gills well suited for gas exchange, these properties leads to costly water and ion fluxes and exposure to toxic substances and pathogens. thus, gill morphology is likely to be a compromise between opposing demands. it has become clear that some fishes have the ability to modify gill structure in response to environmental parameters such as oxygen levels and temperature. maybe the most dramatic example of gill plasticity ... | 2006 | 16540380 |
| the presence of microcystins and other cyanobacterial bioactive peptides in aquatic fauna collected from greek freshwaters. | toxic bloom-forming cyanobacteria can cause animal death and adversely affect human health. blooms may contain microcystins (mcs), cyanobacterial heptapeptide hepatotoxins and other peptides such as anabaenopeptins and anabaenopeptilides. mcs have been shown to occur in various aquatic organisms including mussels, water snails, crustaceans and fish. muscle and viscera samples from eight species of fish (acipenser gueldenstaedtii, carassius auratus, carassius gibelio, cyprinus carpio, perca fluvi ... | 2006 | 16540185 |
| evidence for melano-macrophage centres of teleost as evolutionary precursors of germinal centres of higher vertebrates: an immunohistochemical study. | the melano-macrophage centres (mmcs) of the haemolymphopoietic organs of teleost fish trap and retain antigens and are closely associated with immunoglobulin-secreting cells. the hypothesis that they are the phylogenetic precursors of the germinal centres of higher vertebrates has been questioned due to their apparent lack of organising cells. in this study the immunoreactivity of mmc cells from spleen and kidney of the teleosts cyprinus carpio, odontesthes bonariensis and solea senegalensis to ... | 2006 | 16533606 |
| establishment and characterization of two cell lines from bluefin trevally caranx melampygus. | bluefin trevally caranx melampygus cuvier is a popular game fish and highly valued sea food with potential importance for aquaculture. to help establish this marine animal as an important aquacultural species in hawaii and the pacific and develop in vitro cell culture systems for long-term management and control of potential viral diseases 2 cell lines were established from body muscle (bluefin trevally muscles, btms) and fins (bluefin trevally fins, btf). primary culture of these cells was cond ... | 2006 | 16532601 |
| in vitro effects of insulin-like growth factors and insulin on oocyte maturation and maturation-inducing steroid production in ovarian follicles of common carp, cyprinus carpio. | in vitro germinal vesicle breakdown (gvbd) in cyprinus carpio oocytes was induced by recombinant human insulin-like growth factor-i and -ii (igf-i and igf-ii) and bovine insulin (b-insulin). treatment of postvitellogenic ovarian follicles with igf-i and b-insulin increased concentration of maturation-inducing hormone (mih), 17alpha,20beta-dihydroxy-4-pregnane-3-one (dhp) in the medium. igf-i and igf-ii both and b-insulin induced gvbd in denuded oocytes. igf-i analogue r3 igf-i was more potent th ... | 2006 | 16531089 |
| influence of dispersants on bioconcentration factors of seven organic compounds with different lipophilicities and structures. | seven compounds with different lipophilicities and structures--1,3,5-trichlorobenzene, pentachlorobenzene, acenaphthylene, 1,4-dimethyl-2-(1-methylphenyl)benzene, 4-ethylbiphenyl, 4,4'-dibromobiphenyl, and 1,1,1-trichloro-2,2-bis(4-chlorophenyl)ethane--were subjected to bioconcentration tests in carp at concentrations below the water solubilities of the compounds in the presence or absence of a dispersant (either an organic solvent or a surfactant). the bioconcentration factors (bcfs) of the com ... | 2006 | 16527330 |
| characterization of a novel virus causing a lethal disease in carp and koi. | since 1998 a lethal disease of carp and ornamental koi (cyprinus carpio) has afflicted fisheries in north america, europe, and asia, causing severe economic losses to the fish farming industry. this review summarizes the isolation and identification of the disease-causing agent and describes the currently known molecular characteristics of this newly isolated virus, distinguishing it from other known large dna viruses. in addition, we summarize the clinical and histopathological manifestations o ... | 2006 | 16524920 |
| demonstration of hematopoietic stem cells in ginbuna carp (carassius auratus langsdorfii) kidney. | a model system of ginbuna carp (clonal triploid fish, carassius auratus langsdorfii) and ginbuna-goldfish hybrids (tetraploid fish, having three sets of chromosomes from a triploid clone and a haploid set of chromosomes from goldfish) was employed to demonstrate the presence of hematopoietic stem cells (hscs) in kidney hematopoietic tissues. kidney hematopoietic cells were obtained from ginbuna carp (s3n, donor) and injected into a ginbuna-gold fish hybrid (s4n, recipient) via the caudal sinus. ... | 2006 | 16519942 |
| plasma sampling and freezing procedures influence vitellogenin measurements by enzyme-linked immunoassay in the fathead minnow (pimephales promelas). | the present study compared three different methods for measuring plasma vitellogenin (vtg) in fathead minnow (fhm; pimephales promelas): a procedure using liquid chromatography with electrospray ionization combined with tandem mass spectrometry (lc/esi-ms/ms), and two commercial enzyme-linked immunoassay (elisa) kits using either anti-carp or anti-fhm antibodies. the influence on plasma vtg measurements of using the protease-inhibitor aprotinin during blood sampling and of submitting the plasma ... | 2006 | 16519293 |
| cloning of crucian carp (carassius cuvieri) metallothionein-ii gene and characterization of its gene promoter region. | the genomic dna of crucian carp (carassius cuvieri) metallothionein-ii (ccmt-ii), with its upstream region, was obtained. the sequence analysis of its upstream region revealed several putative cis-acting elements including seven metal regulatory elements (mres), three activator protein 1 (ap1), two glucocorticoid response elements (gres), etc. the seven mres locate into two clusters, a distal cluster with four mres within -800/-600bp from the translation start site and a proximal cluster with th ... | 2006 | 16516146 |
| temperature regulates hypoxia-inducible factor-1 (hif-1) in a poikilothermic vertebrate, crucian carp (carassius carassius). | hypoxia-inducible transcription factor-1 (hif-1) is a master regulator of hypoxia-induced gene responses. to find out whether hif-1 function is involved in gene expression changes associated with temperature acclimation as well as in hypoxia adaptation in poikilotherms, we studied hif-1 dna binding activity and hif-1alpha expression in normoxia and during hypoxia (0.7 mg l(-1) o2) in crucian carp at temperatures of 26, 18 and 8 degrees c. temperature had a marked influence on hif-1 in normoxia. ... | 2006 | 16513925 |
| are dioxin-like contaminants responsible for the eel (anguilla anguilla) drama? | eel populations worldwide are dangerously close to collapsing. our study is the first to show that current levels of dioxin-like contaminants are strong candidates because of their devastating effects on development and survival of eel embryos. female and male silver eels were artificially stimulated to maturation and reproduction by treatment with carp pituitary extracts and hcg, respectively. during maturation of female european silver eels, about 60 g fat per kg eel is incorporated in the ooc ... | 2006 | 16508793 |
| hepatic antioxidant enzymes and total glutathione of cyprinus carpio exposed to three disinfectants, chlorine dioxide, sodium hypochlorite and peracetic acid, for superficial water potabilization. | this study was carried out in order to assess the effects of disinfectant-treatment on antioxidant response of cyprinus carpio l. therefore, enzymatic activities of glutathione s-transferases, glyoxalase i, glyoxalase ii, glutathione peroxidases, glutathione reductase, catalase and total glutathione content of carp liver, exposed to surface water treated with three disinfectants for potabilization, sodium hypochlorite, chlorine dioxide and peracetic acid were investigated. specimens of carp were ... | 2006 | 16504240 |
| changes in thyroid and vitamin a status in mink fed polyhalogenated-aromatic-hydrocarbon-contaminated carp from the saginaw river, michigan, usa. | a study was conducted to determine whether changes in thyroid and vitamin a dynamics were induced in ranch mink exposed to environmentally relevant concentrations of polyhalogenated aromatic hydrocarbons. adult female mink were fed diets that contained 0% (control), 10%, 20%, or 30% wild carp (cyprinus carpio) collected from the saginaw river, michigan, usa. total polychlorinated biphenyls concentrations were 0.03, 0.83, 1.05, and 1.69 mg/kg feed, respectively; the 2,3,7,8-tetrachlorodibenzo-p-d ... | 2006 | 16499904 |
| effects of hexachlorobenzene on antioxidant status of liver and brain of common carp (cyprinus carpio). | hexachlorobenzene (hcb)-induced oxidative damages have been published in rats while the effects have not yet been reported in fishes. juvenile common carps (cyprinus carpio) were exposed to waterborne hcb from 2 to 200 microg l-1 for 5, 10 or 20 days. liver and brain were analyzed for various parameters of oxidative stress. there were no significant changes of glutathione (gsh) content and superoxide dismutase (sod) activity in liver after 5 or 10 days exposure, whereas obvious drops were observ ... | 2006 | 16497358 |
| expression profiles of tcrbeta and cd8alpha mrna correlate with virus-specific cell-mediated cytotoxic activity in ginbuna crucian carp. | our previous studies have demonstrated that virus-specific cell-mediated cytotoxicity of sensitized leukocytes can be induced using clonal ginbuna crucian carp and their syngeneic cell lines. in the present study, we attempt to determine if virus-specific cytotoxic cell populations of fish express cd8alpha and tcrbeta genes. leukocytes from ginbuna crucian carp were separated into four fractions by immunomagnetic separation and density gradient centrifugation: fraction a, leukocytes with a densi ... | 2006 | 16497350 |
| semantic competition between hierarchically related words during speech planning. | there is overwhelming evidence that during speech planning semantically related words become lexically activated and compete for selection with the to-be-produced target word. the vast majority of this evidence stems from studies using the picture-word task, in which a distractor word (e.g., bird) drawn from the same semantic category as the target (e.g., fish) was shown to inhibit the picture-naming response more strongly than did an unrelated distractor word. by contrast, corresponding evidenc ... | 2005 | 16496720 |
| toxicity, accumulation, and retention of zinc by carp under normoxic and hypoxic conditions. | zinc is an essential micronutrient that becomes toxic at elevated concentrations. under hypoxic events, i.e., temporal depletion of oxygen, the toxicity of zn increases for fish, apart from the direct effects of the reduced oxygen levels. this enhanced toxicity currently is explained by the increased ventilation rates under hypoxia, causing a higher water flow over the gills. however, the few experimental studies available on the uptake of heavy metals under hypoxia draw contradictory conclusion ... | 2006 | 16494228 |
| down regulation of bisphenol a glucuronidation in carp during the winter pre-breeding season. | environmental pollution by bisphenol a is prevalent in many rivers. the influence of bisphenol a on the reproductive organs of carp has been demonstrated to be serious, especially in the winter pre-breeding season. although bisphenol a is detoxified as bisphenol a-glucuronide in carp organs, principally the intestine, the seasonal variation in the efficiency of the detoxification is not known. to estimate the seasonal risk of bisphenol a in carp, we investigated seasonal changes in microsomal ud ... | 2006 | 16481053 |
| the effect of oral immuno-stimulation in juvenile carp (cyprinus carpio l.). | the effect of a 2-week period of oral immuno-stimulation from the age of 2 or 6 weeks post-fertilisation (wpf; before and after reaching the ability to produce antibodies) onwards was investigated on various immune functions of the common carp, cyprinus carpio. the immuno-stimulants aeromonas salmonicida lipopolysaccharide, yeast dna (containing unmethylated cpg motifs) or high-m alginate (an extract of algae containing poly-mannuronic acid) were used. the effect of this treatment was studied on ... | 2006 | 16476554 |
| functional analysis on the 5'-flanking region of carp fast skeletal myosin heavy chain genes for their expression at different temperatures. | two types of the fast skeletal myosin heavy chain (myh) genes were cloned from a genomic dna library of carp (cyprinus carpio l.) and named myh10 and myh30, which showed the sequence similarity to the myh cdnas predominantly expressed in carp acclimated to 10 and 30 degrees c, respectively. the 5'-flanking region of about 3 kbp in size each from myh10 and myh30 contained various cis-elements to bind to transcriptional regulatory factors such as myod family and myocyte enhancer factor 2 (mef2) fa ... | 2006 | 16472943 |
| quantitative and qualitative assessment of serum- and inflammatory mediator-induced migration of carp (cyprinus carpio) head kidney neutrophils in vitro. | a quantitative transmigration system, permitting the harvest of transmigrated cells for further analysis, was used to study carp head kidney (hk) granulocyte migration in vitro. pooled carp serum and leukotriene b4 (ltb-4), but not recombinant human c-x-c chemokine ligand 8 (rhcxcl8), recombinant human complement component 5a (rhc5a) or n-formyl-methionyl-leucyl-phenylalanine (fmlp) induced strong migration (up to 70%) of carp hk granulocytes. the transmigrated cells were viable (>or=96%) and un ... | 2006 | 16469507 |
| crucian carp (carassius carassius) vtg monoclonal antibody: development and application. | the vitellogenin (vtg) in fish has been used as an important biomarker for monitoring endocrine disrupting compounds (edcs). this paper reports the development of a new monoclonal antibody (mcab) against the vtg of crucian carp (carassius carassius). the mcab has a molecular weight of 149.4 kda (heavy chain: 53.1 kda; light chain: 21.6 kda), and double diffusion indicated that it belongs to the igg1 subclass. the titer is 10(5)-10(6) and the affinity constant (k(aff)) is 7.0 x 10(8)l/mol, showin ... | 2007 | 16469380 |
| improved uptake of plant-derived ltb-linked proteins in carp gut and induction of specific humoral immune responses upon infeed delivery. | oral vaccination of fish is an effortless and stress free immunisation method which can be used for almost any age. however, vaccination via the mucosal route does have disadvantages. for example, the vaccine may induce tolerance and has to be protected to escape digestion. also the vaccine should be efficiently delivered to immune-competent cells in the gut or other lymphoid organs. in addition, it should be cost effective. here we present a novel fish vaccination model using potato tubers as v ... | 2006 | 16464614 |
| beta-2-microglobulin gene expression is maintained in rainbow trout and atlantic salmon kept at low temperatures. | finfish in the wild are regularly subjected to low temperatures, which have been shown to cause a loss of major histocompatibility receptor expression in common carp kept at 6 degrees c. this is similar to what was seen in a mammalian cell line cultured at 26 degrees c. loss of expression of this critical viral recognition protein may provide one mechanism for the increased frequency of fish diseases at low temperatures. this report demonstrates that unlike carp and mammals, beta(2)m transcript ... | 2006 | 16464613 |
| the presence of multiple and differentially regulated interleukin-12p40 genes in bony fishes signifies an expansion of the vertebrate heterodimeric cytokine family. | interleukin-12 (il-12) is the founding member of a rapidly growing family of heterodimeric cytokines. it consists of two subunits, designated p35 and p40 that together constitute a disulphide-linked heterodimeric cytokine. il-12 is well known for its prominent role in both the early innate immune response and the skewing of the ensuing acquired immune response towards th1. here, we report the presence of il-12p35 and three highly distinct il-12p40 genes in common carp (cyprinus carpio). the carp ... | 2006 | 16460805 |
| evaluation of sugarcane by-product pressmud as a manure in carp culture. | the effectiveness of pressmud, a sugarcane by-product, was tested as a manure in the production of natural food and carp, by conducting two experiments. the first experiment, of 5 weeks duration, examined the effect of pressmud addition on water quality and natural food production. this study was carried out in fibreglass tubs (1.54 m3) with a 5 cm mud layer, employing pressmud at dosages of 5000, 10,000 and 15,000 kg ha(-1) for treatments t1, t2 and t3, respectively. unfertilized tubs served as ... | 2006 | 16459356 |
| changes in sperm motility in response to osmolality/ca2+ in three indonesian fresh water teleosts: goby (oxyeleotris marmorata), java carp (puntius javanicus), and catfish (clarias batrachus). | sperm of most fresh water teleosts become motile when released into the hypotonic fresh water environment, but the role of osmolality and ca2+ on sperm motility is not clear. osmotic pressure and ca2+ concentrations increase from fresh water to brackish water. java carp puntius javanicus and catfish clarias batrachus live and reproduce only in fresh water. on the other hand, goby oxyeleotris marmorata can acclimate and reproduce from fresh water to brackish water. in the present study, sperm mot ... | 2006 | 16458554 |
| sublethal effects of organophosphate diazinon on the brain of cyprinus carpio. | effects of diazinon, at different concentrations and exposure times, were investigated in freshwater fish, cyprinus carpio, to elucidate the possible mode of action on lipid peroxidation together with the inhibitory effect of diazinon on acetylcholinesterase activity and changes in tissue protein levels. cholinesterase inhibition is considered to be a specific biomarker of exposure to organophosphorus pesticides. fish were exposed to 0.0036 microg/l, 0.018 microg/l, and 0.036 microg/l (sublethal ... | 2006 | 16455590 |
| heavy metal profile in five fish species included in human diet, domiciled in the end flow of river neretva (croatia). | concentration of three heavy metals (mercury (hg), lead, (pb), and cadmium, (cd)) and one metalloid (arsenic [as]), were determined by atomic absorption spectrophotometry in five tissues (muscles, liver, kidneys, gills, and gonads) of five fish species (carp-cyprinus carpio, tench-tinca tinca, sval-leuciscus svallizi, gray mullet-mugil cephalus, and eel-anguilla anguilla) taken out from the end fiow (last 20 km) of the river neretva, south croatia, in the wider region of town metković, during th ... | 2006 | 16453065 |
| molecular cdna cloning and analysis of the organization and expression of the il-1beta gene in the nile tilapia, oreochromis niloticus. | the full-length cdna sequence of interleukin-1beta (il-1beta) from the nile tilapia, oreochromis niloticus, was determined by using pcr with primers designed from known fish il-1beta sequences followed by elongation of the 5' and 3' ends using rapid amplification of cdna ends (race). the cdna contains a 92-bp 5' untranslated region (utr), a single open reading frame (orf) of 732 bp that translates into a 243-amino acid molecule, a 341-bp 3' utr with four cytokine rna instability motifs (attta), ... | 2006 | 16451827 |
| dimerization of the cardiac ankyrin protein carp: implications for marp titin-based signaling. | cardiac ankyrin repeat protein (carp) and its two close homologs ankrd2 (arpp) and darp correspond to a conserved gene family of muscle ankyrin repeat proteins (marps). all three genes respond to a variety of stress/strain injury signals with their cytokine-like induction and can associate with the elastic region of titin/connectin. recently, both carp and ankrd2 were observed to be elevated in cardiac diseases as well as muscular dystrophies, implicating their joined signaling in muscle disease ... | 2005 | 16450059 |
| [seasonal distribution of gill parasites in fishes from fee-fishing at guariba, são paulo state, brazil]. | this study was accomplished with the objective of knowing the seasonal distribution of the main species of parasites causes of branchial damages to the fish from fee-fishing at guariba, state of são paulo. this city is situated at 21 degrees 15'22'' s, 48 degrees 18'58'' w, in altitude 595 meters. this research was conducted between april, 1997 to march, 1999. from 408 fishes examined, 29.1% of them were infested by the protozoans e metazoans. leporinus macrocephalus showed the highest parasitis ... | 2005 | 16445870 |
| survival of haplorchis taichui metacercariae in lab-pla, thai traditional food preparation. | lab-pla is a famous kind of thai traditional food in northern and northeasthern thailand. consumption of uncooked lab-pla can lead to trematode infection because cyprinoid fish serve as the 2nd intermediate hosts of trematodes. the preparation of lab-pla can be made in different ways, depending on seasonings used in different places. the effect of the seasonings used in lab-pla on the survival of haplorchis taichui metacercariae were, therefore, determined. the methodology was done by the follow ... | 2005 | 16438192 |
| corticotropin-releasing hormone-receptor 1 (crh-r1) and crh-binding protein (crh-bp) are expressed in the gills and skin of common carp cyprinus carpio l. and respond to acute stress and infection. | we established that corticotropin-releasing hormone (crh), crh-binding protein (crh-bp) and crh-receptor 1 (crh-r1) are expressed in the gills and skin of common carp cyprinus carpio, an early vertebrate. immunoreactive crh was detected in macrophage-like cells in gills and skin, in fibroblasts in the skin and in endothelial cells in the gills. the involvement of the crh system in gills and skin was investigated in response to infection and in an acute restraint stress paradigm. carp were infect ... | 2006 | 16424101 |
| assessment of effects in mink caused by consumption of carp collected from the saginaw river, michigan, usa. | polychlorinated hydrocarbons, including polychlorinated biphenyls (pcbs), polychlorinated dibenzo-p-dioxins (pcdds), and polychlorinated dibenzofurans (pcdfs), are ubiquitous environmental contaminants that bioconcentrate in the food chain. numerous studies have demonstrated mink (mustela vison) to be one of the most sensitive species to this group of compounds. in recent studies, a lesion characterized by osteoinvasion of epithelial cells into the mandible and maxilla of young mink fed diets co ... | 2006 | 16418898 |
| exposure of spermatozoa to duroquinone may impair reproduction of the common carp (cyprinus carpio) through oxidative stress. | toxicity of many waterborne organic contaminants to aquatic organisms is mediated through oxidative damages resulting from the production of reactive oxygen species (ros). using duroquinone as a model ros inducer, we carried out in vitro and in vivo experiments to test the hypothesis that reproduction in common carp (cyprinus carpio) can be impaired through oxidative damage of their spermatozoa. in vitro exposure of fish spermatozoa to 0, 12.5, 25, 50, 100 and 200 microm duroquinone for 2 h show ... | 2006 | 16413940 |
| functional characterization of parvalbumin from the arctic cod (boreogadus saida): similarity in calcium affinity among parvalbumins from polar teleosts. | calcium dissociation constants (kd) were measured as a function of temperature for parvalbumin, a small acidic protein expressed abundantly in fast-twitch muscle, from the arctic cod (boreogadus saida) and compared to values previously determined for antarctic and temperate zone teleosts. estimates of kd were derived independently from fluorometric titrations and calorimetry. in addition, the primary structure of b. saida parvalbumin was determined. calcium kds for parvalbumin from b. saida were ... | 2006 | 16412673 |
| environmental contaminants and biomarker responses in fish from the columbia river and its tributaries: spatial and temporal trends. | fish were collected from 16 sites on rivers in the columbia river basin (crb) from september 1997 to april 1998 to document temporal and spatial trends in the concentrations of accumulative contaminants and to assess contaminant effects on the fish. sites were located on the mainstem of the columbia river and on the snake, willamette, yakima, salmon, and flathead rivers. common carp (cyprinus carpio), black bass (micropterus sp.), and largescale sucker (catostomus macrocheilus) were the targeted ... | 2006 | 16406490 |
| ontogeny of the common carp (cyprinus carpio l.) innate immune system. | the ontogeny of the teleost innate immune system was studied in carp using cellular, histological and quantitative molecular techniques. carp myeloid cells first appeared ventro-lateral of the aorta at 2 days post fertilization (the start of hatching), and subsequently around the sinuses of the vena cardinalis (or posterior blood islet), head kidney and trunk kidney. in addition, the hematopoietic tissue around the sinuses of the vena cardinalis transformed into that of the trunk kidney, which i ... | 2006 | 16406121 |
| crf and stress in fish. | the endocrine stress response is pivotal in vertebrate physiology. the stress hormone cortisol-the end product of the endocrine stress axis-(re-)directs energy flows for optimal performance under conditions where homeostasis may be or become at risk. key players in the continuous adaptation process are corticotropin-releasing factor (crf) from the hypothalamic nucleus preopticus (npo), pituitary adrenocorticotropic hormone (acth) and cortisol produced by the interrenal cells in the headkidney (a ... | 2006 | 16403502 |
| isolation and characterization of visual pigment kinase-related genes in carp retina: polyphyly in grk1 subtypes, grk1a and 1b. | visual pigment is phosphorylated and inactivated after light stimulus. the responsible enzyme is known as rhodopsin kinase or g-protein-coupled receptor kinase 1 (grk1) in rods. we recently showed that the kinase in cones (grk7) has much higher activity than grk1 in rods in carp retina. during the course of these studies, we realized that there are several subtypes of grk1 and grk7. in the present study, therefore, to identify the grk1 and grk7 subtypes expressed in carp photoreceptors, we deter ... | 2005 | 16402022 |
| in vitro toxicity of copper, cadmium, and chromium to isolated hepatocytes from carp, cyprinus carpio l. | 2005 | 16400544 | |
| population dynamics of lernaea cyprinacea (crustacea: copepoda) on four cyprinid species. | a total of 217 barbus graellsii, 54 barbus haasi, 417 cyprinus carpio and 85 leuciscus cephalus captured at 9 sampling stations in the central basin of the llobregat river (ne spain) were studied for lernaea cyprinacea parasitism. prevalence (b. graellsii 39.2, b. haasi 31.5, c. carpio 8.4, l. cephalus 48.2), intensity of infection (b. graellsii range 1 to 39, mean 4.6; b. haasi range 1 to 7, mean 2.6; c. carpio range 1 to 16, mean 3.1; l. cephalus range 1 to 8, mean 2.4), and abundance (b. grae ... | 2005 | 16385816 |
| female morphology and systematic status of philometroides cyprini (nematoda: philometridae), a parasite of carp. | gravid females of philometroides were found under the scales of stock carp cyprinus carpio l. imported from lithuania to the czech republic in the spring of 1997 and kept in a quarantine pond. these specimens and those obtained from pond-cultured carp in russia (moscow district) were used for detailed morphological studies, including scanning electron microscopy. the results confirm that the valid name of this parasite is philometroides cyprini (ishii, 1931), whereas philometra lusii vismanis, 1 ... | 2005 | 16385815 |
| reactivation of koi herpesvirus infections in common carp cyprinus carpio. | two co-habitation studies with common carp were conducted to determine whether latent infections of koi herpesvirus (khv) exist. fish were exposed to khv using 2 different temperature profiles, which induced low and high initial mortality. subsequently, certain groups of fish were co-habited with naive fish while others were not. koi herpesvirus was reactivated in fish from 3 of the 5 experimental tanks. reactivation of the virus occurred regardless of the initial mortality associated with the v ... | 2005 | 16385803 |
| different sensitivity of carp (cyprinus carpio) and rainbow trout (oncorhynchus mykiss) to the immunomodulatory effects of uvb irradiation. | in order to study the sensitivity of two fish species, carp (cyprinus carpio) and rainbow trout (oncorhynchus mykiss), to the immunomodulatory effects of ultraviolet b (uvb) radiation, the fish were exposed to a single uvb dose of 50, 250, 500 or 1,000 mj cm(-2). these species represent different phylogenetic groups of fish, and they differ also in their behaviour inhabitating often dark and turbid (carp) or clear and transparent waters (salmonids). immune responses were studied on day 1 post-ir ... | 2006 | 16376572 |
| monitoring the effects of water pollution on cyprinus carpio in karakaya dam lake, turkey. | karakaya dam lake (kdl) is one of the most important water sources, both for irrigation and fishery, located in eastern part of turkey. this study is concerned with the pollution of the lake contributed by urban, industrial and agricultural activities. the parameters selected for this aim were the enzymes commonly used as biomarkers of environmental pollution. the activity of glutathione s-transferase (gst), carboxylesterase (ce), lactate dehydrogenase (ldh), acid phosphatase (acp) and aspartate ... | 2006 | 16374666 |
| acute dysphagia after carp fish meal: a case report. | an 80-year-old man was admitted to the emergency department with acute dysphagia symptoms of 8 hours' duration. the problem began after a carp fish meal. prominent symptoms included inability to swallow, mild dyspnea, and cough. laboratory evaluation and fiber-optic examination of the upper aerodigestive tract was normal except for accumulation of saliva in the hypopharynx. examination with a flexible esophagoscope revealed a total blockage of the lumen of the lower esophagus due to impaction of ... | 2005 | 16369319 |
| [the cloning and expression analysis of sox9b in cyprinus carpio]. | sox9 gene is one of the important transcription factors involved in developments of many tissues and organs, particularly in sex determination and chondrogenesis. in this article, the genomic dna of cyprinus carpio were amplified using degenerate primers. it was found for the first time that there were two versions of sox9 in c. carpio, sox9a and sox9b with the same deduced amino acid sequences in the hmg box. there is one intron in this box with the length 704bp in sox9a and 616bp in sox9b resp ... | 2005 | 16363119 |
| hif-1alpha and inos levels in crucian carp gills during hypoxia-induced transformation. | hypoxia inducible factor 1 alpha (hif-1alpha) initiates expression of a wide variety of genes, some of which are involved in apoptosis and cell cycle arrest. we have previously shown that crucian carp increases its respiratory surface area 7.5-fold in response to hypoxia. this change is due to apoptosis and cell cycle arrest in specific parts of its gills. here we have characterized crucian carp hif-1alpha, and measured mrna, protein and dna binding levels during hypoxia exposure in crucian carp ... | 2006 | 16362306 |
| molecular cloning and mrna expression analysis of carp embryonic, slow and cardiac myosin heavy chain isoforms. | three embryonic class ii myosin heavy chains (myhs) were cloned from the common carp (cyprinus carpio l.), myhemb1, myhemb2 and myhemb3. myh dna clones were also isolated from the slow muscle of adult carp acclimated to 10 degrees c (myhs10) and 30 degrees c (myhs30). phylogenetic analysis demonstrated that myhemb1 and myhemb2 belonged to the fast skeletal muscle myh clade. by contrast, the sequence of myhemb3 was similar to the adult slow muscle isoforms, myhs10 and myhs30. myhemb1 and myhemb2 ... | 2006 | 16354789 |
| a combined phage display scfv library against myxobolus rotundus infecting crucian carp, carassius auratus auratus (l.), in china. | immunological methods have been developed for the diagnosis of myxobolus rotundus but their use has been limited for the prevention and therapy of this serious parasitic pathogen. phage display antibody libraries are a powerful technique for the development of antibodies to molecules of interest and have advantages over traditional hybridroma approaches. in the present study, four antigen fractions related to m. rotundus were prepared and a combined phage display single-chain antibody fragments ... | 2006 | 16351693 |
| synergistic effect of ultrasound and phthalocyanines on nucleated erythrocytes in vitro. | the synergistic effect of ultrasound (us) and chemicals on cells is known as sonodynamic therapy. in this work, two phthalocyanines (zinc and chloroaluminum) have been tested as potential sonosensitizers for sonodynamic therapy. we studied the effect of us and phthalocyanines on carp erythrocytes, as a nucleated cell model. the level of hemolysis, osmotic fragility, lipid peroxidation and oxidation of hemoglobin were the markers of these reactions. red blood cells were preincubated with phthaloc ... | 2005 | 16344133 |
| fish genomics and biology. | the last common ancestor between fish and mammals dates back to the very origin of the vertebrate lineage and today, half of modern vertebrates are fish. it is thus not surprising that several fish species have played important roles in recent years to advance our understanding of vertebrate genome evolution, to inform us on the structure of human genes, and, somewhat more unexpectedly, to provide leads to understanding the function of genes involved in human diseases. genome sequence comparison ... | 2005 | 16339365 |
| possible implication of macrophages in the regulation of cytochrome p450 activities in carp (cyprinus carpio). | macrophages play a key role in the regulation of cytochrome p450 activity induced by immunostimulants in mammals. we investigated the effects of immunostimulants (lps, dextran sulfate and tilorone) on biotransformation and macrophage activities in carp. the major effect of lps was its capacity to inhibit 3-mc-induced cytochrome p450 activities in the liver and head kidney. basal phase i activities were reduced by tilorone and dextran sulfate in immune organs. tilorone and dextran sulfate differe ... | 2006 | 16337134 |
| use of a probiotic to control lactococcosis and streptococcosis in rainbow trout, oncorhynchus mykiss (walbaum). | from a comparison of 125 bacterial isolates recovered from the digestive tract of rainbow trout, oncorhynchus mykiss, and carp, cyprinus sp., a culture was obtained which was effective at preventing clinical disease caused by lactococcus garvieae and streptococcus iniae when used as a feed additive. the culture, aeromonas sobria gc2, was incorporated into the feed and fed to rainbow trout (average weight = 20 g) for 14 days at a dose equivalent to 5 x 10(7) cells g(-1) of feed. whereas the untre ... | 2005 | 16336470 |
| seasonal ovarian cycle in freshwater teleost, labeo rohita (ham.) in tarai region of uttaranchal. | seasonal ovarian cycle, based on histophysiological changes, and correlative variations in liver were studied in an indian major carp, labeo rohita reared in captivity under ambient environmental conditions in tarai region of uttaranchal. the oocyte features used for marking ovarian cycle included nuclear-ooplasmic ratio, yolk vesicle incorporation into ooplasm, number and size of nucleolus and differentiation of follicular layer. based on these features the ovarian cycle in l. rohita was divide ... | 2005 | 16334297 |
| behavioural and hematological responses of cyprinus carpio exposed to mercurial chloride. | the static bioassay experiments were conducted to study the hematological and behavioural responses of cyprinus carpio after exposure to mercuric chloride. three different concentrations i.e. 0.5, 1.0 and 1.5 ppm of mercuric chloride (hgcl2) were used for 8, 16, 24, 48, 72, 96 and 124 hrs for evaluating behavioural responses. hematological responses were assessed after 60 days exposure to 0.1 ppm of hgcl2. the changes in body colour, movement, sluggishness or activeness, disbalance etc. constitu ... | 2005 | 16334273 |
| induction of cyp1a mrna in carp (cyprinus carpio) from the kalamazoo river polychlorinated biphenyl-contaminated superfund site and in a laboratory study. | the kalamazoo river superfund site in michigan is contaminated with polychlorinated biphenyls (pcbs), which were heavily discharged into the river from several paper companies as part of the deinking process in the 1950s through 1970s. we characterized biomarkers of chronic pcb exposure in a resident fish population using real-time reverse transcriptase-polymerase chain reaction to examine mrna expression levels of multiple genes in carp (cyprinus carpio) liver from pcb contaminated and referenc ... | 2006 | 16328624 |
| an in vitro study of long-term potentiation in the carp (cyprinus carpio l.) olfactory bulb. | long-term potentiation (ltp) of synaptic transmission is considered a cellular mechanism for neural plasticity and memory formation. previously, we showed that in the carp olfactory bulb, ltp occurs at the dendrodendritic mitral-to-granule cell synapse following tetanic electrical stimulation applied to the olfactory tract, and suggested that it is involved in the process of olfactory memory formation. as a first step towards understanding mechanisms underlying plasticity at this synapse, we exa ... | 2006 | 16328534 |
| the ontogeny of mucosal immune cells in common carp (cyprinus carpio l.). | the ontogeny of carp (cyprinus carpio l.) immune cells was studied in mucosal organs (intestine, gills and skin) using the monoclonal antibodies wcl38 (intraepithelial lymphocytes), wcl15 (monocytes/macrophages) and wci12 (b cells). in addition, recombination activating gene 1 expression was examined in the intestine with real time quantitative pcr and in situ hybridization to investigate extrathymic generation of lymphocytes. wcl38(+) intraepithelial lymphocytes (putative t cells) appeared in t ... | 2006 | 16328358 |
| effects of light on trace elements and triiodothyronine levels in plasma of mirror carp (cyprinus carpio). | the changes of copper, zinc, iron, magnesium, manganese, and triiodothyronine as a result of short- and long-term isothermal exposure to light were determined in the plasma of mirror carp (cyprinus carpio). two groups of fish were subject to a 3-mo treatment that consisted of (1) 8 h of light followed by 16 h of darkness and (2) 16 h of light followed by 8 h of darkness at 25 degrees c. no significant changes were recorded between the two groups at the beginning of the study. after 3 mo, the tri ... | 2005 | 16327068 |
| adjuvant effect of propolis on immunisation by inactivated aeromonas hydrophila in carp (carassius auratus gibelio). | 2006 | 16325428 | |
| the tapeworm ligula intestinalis (cestoda: pseudophyllidea) inhibits lh expression and puberty in its teleost host, rutilus rutilus. | the tapeworm ligula intestinalis occurs in the body cavity of its cyprinid second intermediate host, in this study the roach rutilus rutilus, and inhibits host gonadal development. the mechanism by which infected fish are prevented from reproducing is unknown. comparison of parameters, such as body length and weight, and condition factor and age, between infected and uninfected individuals, indicated only minor effects of parasitism on growth and condition. in contrast, seasonal gonadal developm ... | 2005 | 16322554 |
| evidence for recombination of mitochondrial dna in triploid crucian carp. | in this study, we report the complete mitochondrial dna (mtdna) sequences of the allotetraploid and triploid crucian carp and compare the complete mtdna sequences between the triploid crucian carp and its female parent japanese crucian carp and between the triploid crucian carp and its male parent allotetraploid. our results indicate that the complete mtdna nucleotide identity (98%) between the triploid crucian carp and its male parent allotetraploid was higher than that (93%) between the triplo ... | 2006 | 16322508 |
| gender distinction in neural discrimination of sex pheromones in the olfactory bulb of crucian carp, carassius carassius. | studies on projection of the sensory neurons onto the olfactory bulb in fish have revealed a clear subdivision into spatially different areas that each responded specifically to different classes of odorants. amino acids induce activity in the lateral part, bile salts induce activity in the medial part, and alarm substances induce activity in the posterior part of the medial olfactory bulb. in the present study, we demonstrate a new feature of the bulbar chemotopy showing that neurons specifical ... | 2006 | 16322086 |
| assessment of mosquito larvicidal potency of cyclic lipopeptides produced by bacillus subtilis strains. | in this study, mosquito larvicidal potency of cyclic lipopeptides (clps) secreted by two bacillus subtilis strains were determined. lc50 of the crude clps secreted by b. subtilis dm-03 and dm-04 strains against third instar larvae of culex quinquefasciatus was 120.0+/-5.0 and 300.0+/-8.0mg/l respectively post 24 h of treatment. physico-chemical factors such as ph of water, incubation temperature, heating and exposure to sunlight hardly influenced the larvicidal potency of these clps. present stu ... | 2006 | 16316617 |
| structure-activity relationship of neuropeptide gamma derived from mammalian and fish. | this study of relationship between structure and biologic activity was performed using five neuropeptide gammas [npgamma; mammalian-npgamma (m-npgamma), trout-npgamma (t-npgamma), goldfish-npgamma (g-npgamma), bowfin-npgamma (b-npgamma), and shark-npgamma (s-npgamma)]. circular dichroism (cd) spectra showed that all peptides took random structure in buffer solution. in neutral and acidic liposomes, m-npgamma, t-npgamma, b-npgamma, and s-npgamma still adopted random structure, while g-npgamma had ... | 2005 | 16316456 |
| reference values for exposure to pah contaminants: comparison of fish from ohio and mid-atlantic streams. | reference values for background exposures of benzo[a]pyrene (bap)- and naphthalene (naph)-type metabolites were calculated for white sucker, northern hog sucker, and rock bass from the mid-atlantic region based on the 90th percentile of a probability-based sample. preliminary findings are presented for common carp. bile was collected from fish and assayed for polycyclic aromatic hydrocarbon metabolites using the fluorescent method of lin et al. exposure reference values for white sucker were 0.3 ... | 2006 | 16314983 |
| a battery of in vivo and in vitro tests useful for genotoxic pollutant detection in surface waters. | since the 1980s, stricter water quality regulations have been promulgated in many countries throughout the world. we discuss the application of a battery of both in vivo and in vitro genotoxicity tests on lake water as a tool for a more complete assessment of surface water quality. the lake water concentrated by adsorption on c18 silica cartridges were used for the following in vitro biological assays: gene conversion, point mutation, mitochondrial dna mutability assays on the diploid saccharomy ... | 2006 | 16313981 |
| distribution of persistent organochlorine pesticides in tissue/organ of silver carp (hypophthalmichthys molitrix) from guanting reservoir, china. | the concentration of organochlorine pesticides (ocps) in tissues and organs of silver carp (hypophthalmichthys molitrix) from guanting reservoir were investigated to evaluate the pollution potential and distribution of ocps. a total of 16 ocps were measured and the concentrations were in the range of 1.61-69.01 ng/g wet weight (ww) for total ocps, 0.16-0.75 ng/g ww for. hcb, 0.75-26.80 ng/g ww for sigmahch (sum of alpha-, beta-, gamma- and delta-hch) and 0.68-35.94 ng/g ww for sigmaddt( sum of p ... | 2005 | 16312991 |
| [koi herpesvirus disease]. | koi herpesvirus (khv) disease emerged at the late 1990s, and has rapidly spread to the world. in japan, khv disease first occurred at october 2003. the disease resulted in mass mortality of wild carp as well as cultured carp. until now, khv-infected carp were found in 42 out of 47 prefectures in japan. only carp cyprinus carpio is susceptible to khv, while goldfish, closely-related species to carp, is not. the affected carp swim lethargically. sunken eyes and gill necrosis are frequently noticed ... | 2005 | 16308541 |
| specific projection of the sensory crypt cells in the olfactory system in crucian carp, carassius carassius. | to study the projection of a special type of sensory neuron called crypt cells in the olfactory system in crucian carp, carassius carassius, we applied the neural tracer 1,1-dilinoleyl-3,3,3',3'-tetramethylindocarbocyanine perchlorate (dii) in the olfactory bulb (ob). small crystals of dii were applied in a small area at the synaptic region at the ventral part of the ob, where a population of secondary neurons specific for sex pheromones has been identified. in those samples (4 out of 24) where ... | 2006 | 16306315 |
| algorithms for selecting informative marker panels for population assignment. | given a set of potential source populations, genotypes of an individual of unknown origin at a collection of markers can be used to predict the correct source population of the individual. for improved efficiency, informative markers can be chosen from a larger set of markers to maximize the accuracy of this prediction. however, selecting the loci that are individually most informative does not necessarily produce the optimal panel. here, using genotypes from eight species--carp, cat, chicken, d ... | 2005 | 16305328 |
| autoradiographic localization and binding study of benzodiazepines receptor sites in carp brain (cyprinus carpio l.). | this study demonstrates, for the first time, by both autoradiography and binding assay that [3h]ro 15-1788 binds to carp brain with a high degree of anatomical selectivity. saturation binding of the radioligand was determined in seven anatomically defined regions and suggested the presence of one class of binding sites (type i-lke). in general, there was a good correlation between the autoradiographic and the binding data. by far, the optic tectum and the vagal, facial, and glossopharyngeal lobe ... | 2006 | 16298509 |
| the in vitro effects of some pesticides on carbonic anhydrase activity of oncorhynchus mykiss and cyprinus carpio carpio fish. | systemic carbonic anhydrase (ca) inhibitors are among the most powerful agents to lower intraocular pressure. unfortunately, their use is frequently accompanied by undesired side effects. some are due to the relatively large amounts of drug that have to be systematically administered to inhibit the ca in the ciliary processes. the aim of the present work was to study in vitro effects of some pesticides on ca enzyme obtained from blood of fish, which play a key role in salt- and osmoregulation an ... | 2006 | 16298474 |
| the immunomodulatory effects of tuftsin on the non-specific immune system of indian major carp, labeo rohita. | the purpose of this study was to determine if injections of different dosages of tuftsin would enhance the immune response and disease resistance against the infections due to the opportunistic pathogens aeromonas hydrophila and edwardsiella tarda in labeo rohita fingerlings. hence, four different dosages of tuftsin in pbs suspension at the rate of 0, 5, 10, 15 mg kg(-1) body weight of fish were injected intraperitoneally to the fingerlings of l. rohita at 2-week intervals for four times. after ... | 2006 | 16293422 |
| genomic organization, gene duplication, and expression analysis of interleukin-1beta in channel catfish (ictalurus punctatus). | interleukin-1beta (il-1beta) is one of the pivotal early response pro-inflammatory cytokines that enables organisms to respond to infection and induces a cascade of reactions leading to inflammation. in spite of its importance and two decades of studies in the mammalian species, genes encoding il-1beta were not identified from non-mammalian species until recently. recent research, particularly with genomic approaches, has led to sequencing of il-1beta from many species. clinical studies also sug ... | 2006 | 16280165 |
| arsenic speciation in farmed hungarian freshwater fish. | arsenic speciation analysis was carried out on freshwater farmed fish collected from an area with elevated groundwater arsenic concentrations in hungary as well as from outside of the area (control samples). the arsenic species were determined by high-performance liquid chromatography-inductively coupled plasma mass spectrometry on methanol extracts of the muscle tissue from the fish. catfish (claries gariepinus) were raised in geothermal water where the average total arsenic concentrations were ... | 2005 | 16277428 |
| sensitive apoptosis induced by microcystins in the crucian carp (carassius auratus) lymphocytes in vitro. | microcystins including leucine-arginine l-amino acid (mclr) and arginine-arginine l-amino acid (mcrr) can inhibit several serine/threonine protein phosphatases. in this study, we focused on the efficient biomarker for analyzing toxic cyanobacteria blooms using in vitro apoptosis bioassay. we explored the existence of sensitive apoptosis induced by mclr and mcrr on isolated lymphocytes of the crucian carp (carassius auratus) at a low exposure level. apoptosis was detected in vitro and was clearly ... | 2006 | 16271850 |
| preservative effect of combined treatment with electrolyzed nacl solutions and essential oil compounds on carp fillets during convectional air-drying. | the antimicrobial and antioxidant effects on carp fillet samples of treatments with alkaline electrolyzed nacl solution ew (-) prior to treatment with acidic electrolyzed nacl solution ew (+) and 1% solutions of the essential oils consisting of 0.5% carvacrol and 0.5% thymol (1% cv+ty) were tested. first carp fillet samples were treated with ew (-), then ew (+), followed by 1% (c+t), represented as [ew (-)/ew (+)/1% (cv+ty)] for 15 min, during drying at 45 degrees c. samples were subsequently ev ... | 2006 | 16271412 |
| detection of carp interstitial nephritis and gill necrosis virus in fish droppings. | carp interstitial nephritis and gill necrosis virus (cngv) is an unclassified large dna virus that morphologically resembles members of the herpesviridae but contains a large (ca. approximately 280-kbp) linear double-stranded dna. this virus has also been named koi herpesvirus, koi herpes-like virus, and cyprinid herpesvirus 3. cngv is the cause of a lethal disease that afflicts common carp and koi. by using immunohistochemistry, molecular analysis, and electron microscopy we previously demonstr ... | 2005 | 16269770 |
| spermatozoal competition in common carp (cyprinus carpio): what is the primary determinant of competition success? | the percentage of sperm motility (92-100%), spermatozoan velocity (112-163 microm.s(-1)) and control hatching rates (83-96%) were evaluated for each of six gold and five green male common carp (cyprinus carpio). in all 30 possible paired combinations of sperm-competition tests, hatching rates of 90-97% were achieved. the mean percentage of offspring sired was strongly influenced by the male used (p < 0.001, r2 = 0.91). the best male sired an average of 88% of the offspring in its competition tes ... | 2005 | 16264099 |
| evolutionary conservation of alternative activation of macrophages: structural and functional characterization of arginase 1 and 2 in carp (cyprinus carpio l.). | classically activated macrophages (camf) play an important role in type-i immune responses and alternatively activated macrophages (aamf) function in type-ii immune responses. while the classical activation of fish macrophages has been well described, the existence of aamf has not yet been described for teleosts. arginase is the characteristic enzyme in aamf and two isoforms have been described for mammals. to study the presence of aamf in a primitive vertebrate species we cloned arginase 1 and ... | 2006 | 16257446 |
| polar filament discharge of myxobolus cerebralis actinospores is triggered by combined non-specific mechanical and chemical cues. | this study presents initial evidence for the requirement of both chemical and mechanical stimuli to discharge polar capsules of myxobolus cerebralis actinospores, the causative agent of salmonid whirling disease. the obligate need for combined discharge triggers was concluded from data obtained in a before/after experimental set-up carried out with individual locally immobilized actinospores. homogenized rainbow trout mucus as chemostimulus and tangency of the apical region of the spores to achi ... | 2005 | 16255819 |
| quantitative structure-toxicity relationships of organophosphorous pesticides to fish (cyprinus carpio). | this study was conducted to determine the relationships between 1381 chemical and structural parameters of 43 organophosphorus pesticides (ops) and their toxicity to fish, cyprinus carpio, using chemoffice 8.03 and dragon 2.1. by multivariate linear regression and intervariable regression analyses, various equations have been derived to calculate the lethal toxicity value, lc(50), for 43 ops found in fish with different levels of toxicity. results show that for all selected ops, especially those ... | 2006 | 16246398 |
| an overview of health control in croatian aquaculture. | aquaculture is an important segment of croatian fishing industry with long tradition of carp and rainbow trout farming as well as marine aquaculture represented by shellfish (flat oyster and mussels), sea bass and sea bream cultivation and atlantic bluefin tuna fattening. the fish and shellfish diseases survey is regulated by "decree on the measures of animal health protection against infectious and parasite diseases" issued yearly by ministry of agriculture. this report derives from systematic ... | 2005 | 16244940 |
| functional divergence of duplicated c-myc genes in a tetraploid fish, the common carp (cyprinus carpio). | the proto-oncogene c-myc is thought to be one of the most important genes in controlling cell proliferation. in a tetraploid fish, two c-myc genes (cam1 and cam2) were previously isolated from the common carp, cyprinus carpio, and were shown to have different expression patterns in adult tissues. here we found that cam1 and cam2 proteins had distinct properties in terms of their transcription regulation system, formation of the transcription activator complex myc/max, and transcriptional activat ... | 2005 | 16242865 |
| stress-dependent and -independent expression of the myogenic regulatory factors and the marp genes after eccentric contractions in rats. | the relationship between muscle mechanical conditions and gene expression was investigated by varying both stress and contraction mode imposed upon rat dorsiflexors (n= 25), activating them at high or low frequencies (150 hz or 40 hz) either eccentrically or isometrically. muscle physiological, immunohistochemical and gene expression changes were then measured 24 h after the exercise bout. peak stress was the best predictor of muscle injury, independent of contraction mode (i.e. eccentric or iso ... | 2006 | 16239282 |
| modulation by melatonin of glutamatergic synaptic transmission in the carp retina. | melatonin is involved in a variety of physiological functions through activating specific receptors coupled to gtp-binding protein. melatonin and its receptors are abundant in the retina. here we show for the first time that melatonin modulates glutamatergic synaptic transmission from cones to horizontal cells (hcs) in carp retina. immunocytochemical data revealed the expression of the mt1 receptor on carp hcs. whole-cell recordings further showed that melatonin of physiological concentrations p ... | 2005 | 16239269 |
| regulation of exogenous gene expression in fish cells: an evaluation of different versions of the tetracycline-regulated system. | the exogenous control of foreign gene expression is relevant to both basic research and biotechnological applications. in fish, the number of isolated genes has become larger in the last few years; however an efficient system for controlling gene expression is not yet available. the tetracycline-regulated system has proved to be efficient and it is widely used in mammals, but it has never been tested in fish. this work includes the establishment of the tetracycline-regulated system for use in fi ... | 2005 | 16236467 |
| treatment of aquarium water by denitrifying photosynthetic bacteria using immobilized polyvinyl alcohol beads. | during the purification of an aquarium for carp breeding, a relatively high level of chemical oxygen demand (cod) was removed by filtration systems packed with both alginate- and polyvinyl alcohol (pva)-immobilized gel beads of rhodobacter sphaeroides s. low nitrate accumulation was observed in the alginate gel beads packed system due to denitrification, but high levels of nitrate and nitrite accumulation were observed in the pva gel beads packed system. this phenomenon was caused by the inhibit ... | 1999 | 16232449 |
| the in vitro effects of cpg oligodeoxynucleotides on the expression of cytokine genes in the common carp (cyprinus carpio l.) head kidney cells. | synthetic oligodeoxynucleotides with cpg motifs (cpg-odns) have gained attention because it elicits strong innate immune responses. cpg-odns promote the production of t-helper 1(t(h)1) and pro-inflammatory cytokines. in fish, knowledge of the effects of cpg-odns on the expression of cytokine genes is scarce. in this study, we report that cpg-odns induce the expression of pro-inflammatory cytokines in common carp head kidney leucocytes in vitro. evidence of a t(h)1 type immune stimulation was obs ... | 2006 | 16229898 |
| environmental contaminants and biomarker responses in fish from the rio grande and its u.s. tributaries: spatial and temporal trends. | we collected, examined, and analyzed 368 fish of seven species from 10 sites on rivers of the rio grande basin (rgb) during late 1997 and early 1998 to document temporal and geographic trends in the concentrations of accumulative contaminants and to assess contaminant effects on the fish. sites were located on the mainstem of the rio grande and on the arroyo colorado and pecos river in texas (tx), new mexico (nm), and colorado. common carp (cyprinus carpio) and largemouth bass (micropterus salmo ... | 2005 | 16227080 |
| acute toxicity of dichlorvos (ddvp) to fingerling mirror carp, cyprinus carpio l. | 2005 | 16222512 | |
| efficient expression of transgenes in adult zebrafish by electroporation. | expression of transgenes in muscle by injection of naked dna is widely practiced. application of electrical pulses at the site of injection was demonstrated to improve transgene expression in muscle tissue. zebrafish is a precious model to investigate developmental biology in vertebrates. in this study we investigated the effect of electroporation on expression of transgenes in 3-6 month old adult zebrafish. | 2005 | 16221312 |