Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| sites of release of airborne foot-and-mouth disease virus from infected pigs. | pigs infected with foot-and-mouth disease virus by different routes of exposure were air-sampled individually, first as 'intact' (i-) pigs and then as 'intubated' (t-) pigs, using an endotracheal tube. irrespective of the route of infection it was found that during the early stages of disease more virus was recovered from i-pigs than from t-pigs. most of the virus from i-pigs during incubation and early disease was associated with large and medium sized particles. t-pigs infected by direct or in ... | 1980 | 6265993 |
| differential precipitation of foot and mouth disease virus proteins made in vivo and in vitro by hyperimmune and virus particle guinea pig antisera. | 1981 | 6264693 | |
| nucleotide sequence and corresponding amino acid sequence of the gene for the major antigen of foot and mouth disease virus. | a segment of 1160 nucleotides of the fmdv genome has been sequenced using three overlapping fragments of cloned cdna from fmdv strain o1k. this sequence contains the coding sequence for the viral capsid protein vp1 as shown by its homology to known and newly determined amino acid sequences from this man antigenic polypeptide of the fmdv virion. the structural gene for vp1 comprises 639 nucleotides which specify a sequence of 213 amino acids for the vp1 protein. the coding sequence is not flanked ... | 1981 | 6264400 |
| visualization by chilling of protein bands in polyacrylamide gels containing 8 m urea: preparation and quantitation of foot-and-mouth disease virus capsid proteins. | 1981 | 6263128 | |
| isolation of a soluble and template-dependent foot-and-mouth disease virus rna polymerase. | 1981 | 6263001 | |
| molecular cloning of foot and mouth disease virus genome and nucleotide sequences in the structural protein genes. | foot and mouth disease virus (fmdv), of the family picornaviridae, consists of a single-stranded rna (approximately 8,000 nucleotides), the translation of which is initiated on the 3' side of a 150-nucleotide poly(c) tract and yields a single polyprotein which is processed by host cell proteases into four primary products (fig. 1). one or more virus-specified proteases further cleave these into the final products, the capsid proteins (vp1-4) being derived from the precursor p88 (for review see r ... | 1981 | 6261157 |
| nucleotide sequence heterogeneity of the rna from a natural population of foot-and-mouth-disease virus. | the genomic rna from isolates of foot-and-mouth-disease virus (fmdv) of serological types o or c obtained during epizootic outbreaks have been analysed by two-dimensional gel electrophoresis of the t1 rnase-generated oligonucleotides (t1 fingerprinting). among virus isolates that are closely related serologically, 4-12 oligonucleotide changes were detected constitute the genome, the variations affect 0.7%-2.2% positions in fmdv rna. higher nucleotide-sequence divergence exists between the genomi ... | 1980 | 6260578 |
| cloning of cdna of major antigen of foot and mouth disease virus and expression in e. coli. | double-stranded dna copies of the single-stranded genomic rna of foot and mouth disease virus have been cloned into the escherichia coli plasmid pbr322. a restriction map of the viral genome was established and aligned with the biochemical map of foot and mouth disease virus. the coding sequence for structural protein vp1, the major antigen of the virus, was identified and inserted into a plasmid vector where the expression of this sequence is under control of the phage lambda pl promoter. in an ... | 1981 | 6258084 |
| comparison of the nucleotide sequence at the 5' end of rnas from nine aphthoviruses, including representatives of the seven serotypes. | the sequence of about 70 nucleotides at the 5' end of the rnas of nine different aphthoviruses (foot-and-mouth disease viruses), including representatives of the seven serotypes of the virus, has been determined by partial enzyme digestion of (32)p-end-labeled s fragment-that part of the rna lying to the 5' side of the poly(c) tract and including the 5' end of the molecule. the s fragments were prepared from polyadenylated virus-specific rna extracted from infected cells by digestion with rnase ... | 1980 | 6257918 |
| a study of the cross-reacting antigens on the intact foot-and-mouth disease virus and its 12s subunits with antisera against the structural proteins. | cross-reactions between two strains of foot-and-mouth disease virus (fmdv) belonging to different serotypes (a and o) were studied with intact virus and virus subunits and antisera produced against the isolated structural proteins. anti-vp1 type o serum showed cross-reactive neutralizing activity, in contrast to the sera raised against intact virus type o, whereas anti-vp1 type a serum only neutralized homologous virus. anti-vp2, vp3 and vp4 did not show neutralizing activity. in the enzyme-link ... | 1980 | 6257830 |
| a study of the level of nucleotide sequence conservation between the rnas of two types serotypes of foot-and-mouth disease virus. | the level of nucleotide sequence conservation between the rnas of type a and type o foot-and-mouth disease virus (fmdv) has been compared by three methods. (i) rna hybridization of fragments containing either the poly(c) tract at the 5' end of the rna of the poly(a) tract at the 3' end of the rna indicates that there is a similar level of sequence conservation (65% homology) across the genome. rnase t1 fingerprinting of these fragments did not show the presence of any long regions of completely ... | 1980 | 6257826 |
| serological and immunological relations between the 146s and 12s particles of foot-and-mouth disease virus. | intact 146s particles of the seven serotypes of foot-and-mouth disease virus (fmdv) produce type-specific precipitating, complement-fixing and neutralizing antibodies in cattle and guinea-pigs. however, the 12s structural subunit, produced from the virus particle by mild acid treatment (ph 6) or by heating at 56 degrees c, although stimulating the production of precipitating and complement-fixing antibodies, produces only low levels of neutralizing antibody. nevertheless, 12s antibody in guinea- ... | 1980 | 6257825 |
| detection and quantification of igm, iga, igg1 and igg2 antibodies against foot-and-mouth disease virus from bovine sera using an enzyme-linked immunosorbent assay. | a simple solid-phase enzyme immunoassay is described for the detection of antibody classes showing activity against foot-and-mouth disease (fmd) virus in bovine sera. the assay achieves a preliminary separation of the specific class of antibody from other serum proteins through immuno-adsorption to class-specific immunoglobulin-coated wells of micro-titre plates. the specific antibody is reacted with fmd virus, which is then detected by an enzyme-labelled anti virus igg. | 1981 | 6257779 |
| morphogenesis of vesiculation in foot-and-mouth disease. | the morphogenesis of vesiculation in cattle inoculated (aerosol expsoure) with foot-and-mouth disease virus was investigated by examining alternate, frozen sections of selected tissues stained by fluorescent antibody technique and with hematoxylin and eosin. viremia preceded the development of lesions, and virus appeared to be transported to the epithelium via papillae. lesions were initiated usually by the infection of single cells in the stratum spinosum adjacent to the papillae. three types o ... | 1980 | 6255841 |
| foot and mouth disease virus. properties of a clone selected by terminal dilution technique from the original population. | using the terminal dilution technique, a cloned virus was selected in a tissue culture systems from a heterogenous population of the original virus. the different markers studied differentiated the cloned virus from the original virus on the basis of its following characters: 1. ability to grow more efficiently at 29 than at 37 degrees c. 2. unstability of its capsid as seen by: a. high degree of sensitivity to heating at 50 degrees c for 10 min of exposure to ph 6.4 for 10 min. b. more inactiva ... | 1980 | 6254429 |
| some virus diseases of domestic animals in the sultanate of oman. | little is known of the occurrence of animal virus diseases in the sultanate of oman. this paper reports the results of a countrywide survey carried out in 1978 to establish the prevalence of some important viral pathogens of domestic animals with the dual purpose of providing baselines for future investigations and guidelines for those entrusted with disease control. foot-and-mouth disease virus type o, previously identified in oman in 1976, was isolated from clinically affected animals. in addi ... | 1980 | 6251586 |
| isolation of a foot-and-mouth disease polyuridylic acid polymerase and its inhibition by antibody. | a template-dependent polyuridylic acid [poly(u)] polymerase has been isolated from bhk cells infected with foot-and-mouth disease virus (fmdv). enzyme activity in a 20,000 x g supernatant of a cytoplasmic extract was concentrated by precipitation with 30 to 50% saturated ammonium sulfate. the poly(u) polymerase was freed of membranes by sodium dodecyl sulfate and 1,1,2-trichlorotrifluoroethane extraction, and rna was removed by precipitation with 2 m licl. the solubilized poly(u) polymerase requ ... | 1980 | 6251248 |
| detection of antibodies against foot-and-mouth disease virus using purified staphylococcus a protein conjugated with alkaline phosphatase. | purified protein a (pa) from staphylococcus aureus was conjugated with the enzyme alkaline phosphatase. this reagent reacted well with pig, rabbit and guinea pig igg and less well with mouse and bovine igg. radioactive 125i-labelled pa showed a similar affinity for the iggs examined. antibodies against foot-and-mouth disease virus contained in guinea pig, rabbit and pig serum were detected, using the enzyme-conjugated pa in indirect tests, which were of similar sensitivity to those using enzyme- ... | 1980 | 6249867 |
| early interactions of foot-and-mouth disease virus with cultured cells. | 1980 | 6249029 | |
| [the rotating aeration (system fuchs) for treatment of liquid animal and municipal wastes. 8. the effect of aerobic treatment of foot and mouth disease virus]. | 1980 | 6248023 | |
| heterogeneity of the genome-linked protein of foot-and-mouth disease virus. | the genome-linked protein of foot-and-mouth disease virus was examined by electrofocusing in polyacrylamide gels. two proteins of different charge and amino acid composition were found. the tryptic peptide maps of the proteins were dissimilar. the possible relationship between the two proteins is discussed. | 1980 | 6247501 |
| the application of a single radial haemolysis technique to foot-and-mouth disease virus-antibody study. | experiments are described for the evaluation of the single radial haemolysis (srh) technique applied to fmd virus, using a method involving the coupling of either antigen or antibody to sheep erythrocytes. the antigen coupling method detected specific antibody but the technique was found to be impractical as it gave reproducible results only with purified virions. however, the antibody coupling technique gave clear zones of lysis using tissue culture harvest virus as well as purified virus, and ... | 1980 | 6246855 |
| temperature-sensitive mutants of foot-and-mouth disease virus with altered structural polypeptides. ii. comparison of recombination and biochemical maps. | the structural polypeptides of foot-and-mouth disease virus were digested with staphylococcus aureus v8 protease in the presence of sodium dodecyl sulfate. the protease-resistant peptides derived from temperature-sensitive mutants were compared with those of the wild type by electrofocusing in a polyacrylamide gel. covariation between the charge shifts of different peptides indicated that they shared common sequences: only five independent peptides in all were derived from vp1, vp2, and vp3, acc ... | 1980 | 6246263 |
| temperature-sensitive mutants of foot-and-mouth disease virus with altered structural polypeptides. i. identification by electrofocusing. | the structural polypeptides of foot-and-mouth disease virus were analyzed by electrofocusing in a polyacrylamide gel containing 9 m urea. three versions of the technique were used to accomodate the widely differing isoelectric points of the four polypeptides. vp2 was identified by comparing mature virus with procapsids. the selective actions of proteases on virions of two serotypes and on their 12s particles were examined. from this emerged a simple test for distinguishing the similarly sized po ... | 1980 | 6246262 |
| structural proteins of hand, foot and mouth disease viruses. | coxsackievirus a16 (ca16) and enterovirus 71 (e71), which cause hand, foot and mouth disease, were compared with respect to their polypeptide composition by page. only three proteins were resolved for ca16, whereas the four characteristic structural proteins of e71, (vp1, vp2, vp3 and vp4) were separated. the distribution of labeled proteins suggested that the molecular weights of vp2 and vp3 of ca16 were very similar. the smallest protein, vp4, of ca16 and e71 had the same molecular weight, but ... | 1980 | 6246027 |
| variation in the susceptibility of bhk populations and cloned cell lines to three strains of foot-and-mouth disease virus. | bhk monolayer and suspension cell populations maintained in different laboratories were found to vary in their susceptibility to infection with three strains of foot-and-mouth disease virus (fmdv). the susceptibility of parent cell populations was compared with that of individual clones of cells derived from them. the populations tested in this way consisted of a number of cell types, each expressing a different capacity to produce fmdv. the relative numbers of susceptible and insusceptible cell ... | 1980 | 6245630 |
| location of the initiation site for protein synthesis on foot-and-mouth disease virus rna by in vitro translation of defined fragments of the rna. | an mrna-dependent reticulocyte lysate has been used to translate foot-and-mouth disease virus rna in vitro. polypeptides p16, p20a, and p88, which have been shown to be derived from the 5' end of the rna by pactamycin mapping experiments with infected cells, were preferentially synthesized in vitro. removal of vpg, the small protein covalently linked to the 5' end of the genome rna, had no effect on the translation of the rna. the two rna fragments (l and s) produced by specific digestion of the ... | 1980 | 6245254 |
| foot and mouth disease virus production on microcarrier-grown cells. | the industrial usefulness of the production of foot and mouth disease virus with microcarrier grown cells has been tested at a large scale. the vaccines, intended for pigs, prepared with such virus give a good level of immunity against virulent challenges. they are stable and free from side effects. | 1980 | 6245001 |
| the potential of recombination for the production of foot-and-mouth disease virus vaccine strains. | biological proof of recombination in foot-and-mouth disease virus has been provided by studies on a large suite of conditional lethal mutants and a recombination map of approximately 70 mutations has been produced. however, recombination is a highly variable phenomenon and this has cast doubt on its validity and certainly on its applicability to such practical purposes as the production of good vaccine strains. evidence is presented to demonstrate (1) that the genetic map has a good correlation ... | 1980 | 6244999 |
| foot-and-mouth disease virus production in glass sphere based monolayer systems of 0.1 to 100 l medium volume. | the cell and virus productivity of a 3 mm glass sphere based unit process monolayer system was determined at scales of 0.1, 1, 10 and 100 litres medium capacity. enhanced yields were obtained up to the 10l size when compared with control roux bottle cultures. modifications of the operating conditions of the 100l system should bring this into line with the smaller-scale units. | 1980 | 6244996 |
| aspects of heat inactivation of foot-and-mouth disease virus in milk from intramammarily infected susceptible cows. | in skim milk obtained from susceptible cows after intramammary and intravenous inoculation (primary infected milk), foot-and-mouth disease (fmd) virus type o1 was slower inactivated by heat treatment than virus that had been added to pre-exposure skim milk. residual virus infectivity in heated primary infected milk was more efficiently detected in bovine thyroid cell cultures than in secondary pig kidney (pk2) cell cultures. untreated primary infected milk was found to inhibit both fmd-virus and ... | 1980 | 6244342 |
| characterization of a foot-and-mouth disease mutant temperature-sensitive for viral rna synthesis. | a temperature-sensitive (ts) mutant of foot-and-mouth disease virus (fmdv) did not produce rna polymerase activity nor synthesize viral rna when incubated in cells solely at the nonpermissive temperature (38.5 degrees c). infected cells initially incubated at 38.5 degrees c and then shifted down to 33 degrees c synthesized increased amounts of viral rna at earlier times compared to infected cells kept at 33 degrees c throughout, indicating that rna polymerase precursors were synthesized at 38.5 ... | 1980 | 6243922 |
| biochemical aspects of variation in foot-and-mouth disease virus. | the biochemical basis for variation in foot-and-mouth disease virus (fmdv) has been explored by analysis of the virus rna and the virus-induced and structural proteins of three isolates of the virus. two of the isolates were from serotype a and the third was from serotype o. hybridization studies of the rnas showed greater than 80% homology between the two type a viruses and about 65% homology between the two type a viruses and the virus of type o. the ribonuclease t1 maps of the three viruses g ... | 1980 | 6243341 |
| [trials to obtain conjugates for the passive hemagglutination test with chromium chloride and their use in studying foot-and-mouth disease viruses]. | experiments were carried out to produce conjugates for the hemagglutination-inhibition reaction with the aid of chromic chloride. studies with reference f.m.d. viruses and with cft revealed that the antibody erythrocyte diagnostic agents were highly specific, and were stable in storage at +4 degrees c and in freeze-drying. it was found that the conjugates obtained with f.m.d. antigens possessed high specificity, however, were not stable and 7 to 10 days later lost their activity. tests with the ... | 1984 | 6209849 |
| chemical basis of antigenic variation in foot-and-mouth-disease virus. | 1984 | 6208066 | |
| localization of a neutralization epitope of foot-and-mouth disease virus using neutralizing monoclonal antibodies. | neutralizing monoclonal antibodies raised against intact foot-and-mouth disease virus reacted with intact particles and with isolated vp1 from different strains from the same subtype. prior treatment of the virus with either trypsin or with arginine-specific protease abolished recognition of both the virus and of vp1, suggesting the presence of a neutralization epitope in the central region of vp1 cleaved by these two enzymes. a synthetic peptide analogue of part of this region showed poor react ... | 1984 | 6206202 |
| epitopes on foot-and-mouth disease virus outer capsid protein vp1 involved in neutralization and cell attachment. | foot-and-mouth disease virus structural protein vp1 elicits neutralizing and protective antibody and is probably the viral attachment protein which interacts with cellular receptor sites on cultured cells. to study the relationships between epitopes on the molecule related to neutralization and cell attachment, we tested monoclonal antibodies prepared against type a12 virus, isolated a12 vp1, and a cnbr-generated a12 vp1 fragment for neutralization and effect on viral absorption. the antibodies ... | 1984 | 6205165 |
| use of peptide synthesis to probe viral antigens for epitopes to a resolution of a single amino acid. | a procedure is described for rapid concurrent synthesis on solid supports of hundreds of peptides, of sufficient purity to react in an enzyme-linked immunosorbent assay. interaction of synthesized peptides with antibodies is then easily detected without removing them from the support. in this manner an immunogenic epitope of the immunologically important coat protein of foot-and-mouth disease virus (type o1) is located with a resolution of seven amino acids, corresponding to amino acids 146-152 ... | 1984 | 6204335 |
| evaluation of the antigenic variation within type-a foot and mouth disease virus isolates from asia. | the serological interrelationships among 17 type a fmd virus strains from eight asian countries were studied by the two-dimensional microneutralization test. complex direct and indirect relationships were observed. overall, however, the virus strains studied could be classified as belonging to the a22 group on the basis of r value differentiation at p less than 0.01. | 1984 | 6203914 |
| antibodies against a preselected peptide recognize and neutralize foot and mouth disease virus. | a major antibody combining site on foot and mouth disease virus (fmdv) serotype o1k has been identified in a predicted surface helix of viral protein 1 (vp1) between amino acid residues 144 and 159. a hexadecapeptide covering this sequence elicits high titers of antibodies that specifically recognize and neutralize fmdv. the high quality of the immune response is attributed to a particularly stable conformation of the antigenic amino acid sequence, which is most likely an alpha-helix. | 1982 | 6203738 |
| demonstration of neutralizing and non-neutralizing epitopes on the trypsin-sensitive site of foot-and-mouth disease virus. | the isolation of monoclonal antibodies directed against the trypsin-sensitive site on the 140s particle of foot-and-mouth disease virus (fmdv) has enabled the demonstration of at least three distinct epitopes within this site. reaction with two of these resulted in neutralization of virus infectivity. none of the epitopes appeared to be present on the 12s particles, and one of the neutralizing epitopes was sensitive to even milder configurational changes of the particle. | 1984 | 6198448 |
| [isolation of foot-and-mouth disease virus antibodies using affinity chromatography]. | 1983 | 6197566 | |
| the molecular basis of the antigenic variation of foot-and-mouth disease virus. | we have cloned and sequenced the viral protein (vp1)-coding regions of two foot-and-mouth disease virus (fmdv) serotypes (c1 and a5). comparison of the derived amino acid sequences with the known vp1 sequence of fmdv o1k and the two fmdv a subtypes a10 and a12 shows two highly variable regions in the protein, at positions 40-60 and 130-160, as possible antigenic sites. in both variable regions, several sites could be detected where all three sequences of the a subtypes are identical but the thre ... | 1983 | 6194987 |
| comparison of the major antigenic determinants of different serotypes of foot-and-mouth disease virus. | complete nucleotide sequences which code for the capsid protein vp1 of two foot-and-mouth disease virus serotypes, o1campos/brazil/58 and c3indaial/brazil/71, have been determined. ten available vp1 sequences (three serotype o, three serotype c, and four serotype a) were aligned and compared. our evidence suggests that o1bfs/britain/68 and o1k/germany/66 are closely related to o1campos/brazil/58. significant variations were observed between the nucleotide sequences of c3indaial determined by two ... | 1983 | 6194313 |
| synthetic peptides mimic subtype specificity of foot-and-mouth disease virus. | the major immunogen of foot-and-mouth disease virus (fmdv) is located between amino acids 141-160 of the capsid protein vp1. synthetic peptides corresponding to the major immunogenic region give good neutralising antibody responses and protection in guinea pigs. to define more precisely the immunogenic site of the virus, we have examined serological differences between subtypes of the a serotype using synthetic peptides covering the 141-160 region. we show that these synthetic peptides carry det ... | 1983 | 6190676 |
| the influence of normal guinea-pig serum and tissue culture assay system on foot-and-mouth disease virus neutralisation. | the inclusion of normal guinea-pig serum in neutralisation reactions involving foot-and-mouth disease virus (fmdv) increased the neutralisation titre and rate of neutralisation by guinea-pig antiserum derived from animals convalescent from fmdv. such inclusion had little or no effect on neutralisation involving guinea-pig antiserum collected early in infection or early or convalescent bovine antisera. higher neutralisation titres and more rapid neutralisation were found from assay in bovine thyr ... | 1983 | 6189669 |
| the main antigenic determinant detected by neutralizing monoclonal antibodies on the intact foot-and-mouth disease virus particle is absent from isolated vpi. | neutralizing monoclonal antibodies raised against intact foot-and-mouth disease virus type o1 reacted with intact virus and trypsin-treated virus particles. some of the monoclonal antibodies showed a slight but definite reaction with the 12s subunit, but none of them reacted with the isolated capsid protein vp1 or any of the other viral proteins. these results confirm the difference between the neutralizing antigenic determinants exposed on intact virus particle and on isolated capsid protein vp ... | 1983 | 6188800 |
| identification of an exposed region of the immunogenic capsid polypeptide vp1 on foot-and-mouth disease virus. | iodination of intact foot-and-mouth disease virus results in the selective labeling of vp1, substantiating its exposed location on the virion. a comparison of tryptic peptides revealed that a single tyrosine-containing peptide was labeled with iodine on intact or protease-cleaved virus. the labeled peptide from intact and protease-cleaved virus was characterized by molecular weight sizing and sequence analysis. carboxypeptidase digestion of intact vp1, limited trypsin-cleaved vp1, and vp1 purifi ... | 1983 | 6186823 |
| monoclonal antibodies against foot-and-mouth disease virus 146s and 12s particles. | this paper is the first description of monoclonal antibodies specific for foot-and-mouth disease virus (fmdv). using these antibodies, it was possible to distinguish similar and unique antigenic sites on complete (146s) or subunit (12s) virus particles. some of these monoclonal antibodies could also distinguish between 12s subunits made by acid degradation of 146s virus (12sa) and those viral components found in infected cell lysates which sediment in sucrose density gradients as "12s subunits" ... | 1982 | 6186225 |
| synthesis of long dna molecules complementary to foot-and-mouth disease virus rna. | 1982 | 6181010 | |
| stimulation by heterotypic antigens of foot-and-mouth disease virus antibodies in vaccinated cattle. | immunisation of cattle with foot-and-mouth disease virus failed to raise a level of antibody that provides protection against heterotypic challenge. further the 12s substructure, produced from the 146s particle, was ineffective in providing protection against challenge by homotypic virus. these findings suggest considerable antigenic differences in the virus serotypes and between the virus and its substructure. inoculation of homologous 12s and heterologous 1246s and 12s antigens into vaccinated ... | 1982 | 6179141 |
| location and characterization of the antigenic portion of the fmdv immunizing protein. | purified foot-and-mouth disease virus (fmdv) to type o1k was treated with several endopeptidases of differing specificity. the immunizing protein vpthr was cleaved into two detectable fragments by all enzymes except for glutamic acid-specific staphylococcus aureus v8 protease. the longest fragments were generated by mouse submaxillary gland protease and the shortest by trypsin treatment of the intact virion. several fragments, including the peptides resulting from the cyanogen bromide (cnbr) cle ... | 1982 | 6176678 |
| quantification, characterisation and safety testing of foot-and-mouth disease virus antigens eluted from alhydrogel vaccines. | different methods for the elution and the concentration of fmdv-antigen from formaldehyde-inactivated alhydrogel vaccines were compared. with locally produced vaccines the best results were obtained if the antigen was eluted with potassium phosphate buffer and the concentration was performed by ultrafiltration. final recoveries of 50% of the 140-s antigen could be obtained. for innocuity control 1-3 mg of 140-s antigen was eluted from 21 vaccine. sds-polyacrylamide gel electrophoresis and iso-el ... | 1981 | 6176487 |
| neutralization of foot-and-mouth disease virus. i. sensitization of the 140s virion by antibody also reactive with the 12s protein subunit. | the in vitro interaction of foot-and-mouth disease virus (fmdv) with an immune serum resulted in a fraction of virus which failed to be neutralized. this inability of antibody to neutralize the entire population of a virus preparation was studied with emphasis on the antigenic specificity of the antibody-virus reaction. antibody to fmdv recognized multiple antigenic determinants. immunoabsorbent fractionation of the serum into 12s subunit cross-reactive and 140s virion-specific antibody revealed ... | 1981 | 6169797 |
| [enzymatic synthesis and properties of dna, complementary to foot and mouth disease virus rna]. | the rnas of the aphthosa virus, serotypes a and o were isolated and characterized. the complementary dnas used in experiments on molecular hybridization with matrix rnas were synthesized on virion rnas by means of reverse transcriptase. | 1980 | 6165404 |
| transformation and foot and mouth disease virus (fmdv) productivity of some bhk cell lines. | 1983 | 6140842 | |
| subtyping of foot-and-mouth disease virus by the micro-enzyme-labelled immunosorbent assay (microelisa). | the micro-enzyme-labelled immunosorbent assay (microelisa) was used successfully for the subtyping of foot-and-mouth disease (fmd) virus strains recovered from field outbreaks. the rabbit anti-guinea pig globulin-peroxidase conjugate employed in the indirect microelisa has the advantage of being used with any of the seven types of fmd virus. | 1981 | 6112867 |
| a micro-enzyme-lavelled immunosorbent assay (micorelisa) for the detection of foot-and-mouth disease virus antigen and antibody. | the indirect technique of a micro-enzyme-labelled immunosorbent assay (microelisa) was standardized and found efficient in detecting the foot-and-mouth disease virus antigen in cell culture fluids, mice carcases and cattle tongue epithelium as well as the antibody titre of sera. | 1981 | 6112866 |
| [early stages in the replication of aphthous fever virus. kinetics of partial degradation and sites for the establishment of penetrating residual virus]. | at input multiplicities between 5-10 ufp/cell fmdv o1 caseros adsorb in bhk21 clon 13 cells at a rate of 5-20% depending whether cells are in suspension or in monolayers. at least four washings with medium are required to eliminate non specifically adsorbed virions. the remaining attached virus appears to be in contact with "specific" receptors of the cytoplasmic membrane. after penetration, 80% of virus became degraded to slow molecular weight material which is detected at first in the subcellu ... | 1982 | 6101001 |
| mimicking foot-and-mouth disease virus antigens with synthetic peptides. | 1984 | 6100728 | |
| response of sheep vaccinated with large doses of vaccine to challenge by airborne foot and mouth disease virus. | administration of three-fold or six-fold larger doses of conventional monovalent type o foot and mouth disease (fmd) vaccine to sheep prevented viraemic distribution of virus after exposure to airborne virus one week later. however, virus replication in the respiratory tract or excretion in oesophageal-pharyngeal fluids and breath was not prevented. the implication of these findings for the use of vaccine as an adjunct to a 'stamping out' policy for countries which are free from fmd and which do ... | 1984 | 6099647 |
| computerization of foot and mouth disease virus strain relationship data. | since 1976 the two dimensional microneutralization test has been adopted in our laboratory as the reference test for foot and mouth disease virus (fmdv) strain differentiation. large numbers of homologous-heterologous comparisons have been performed and manual storage and retrieval of the data had become cumbersome. the objective of computerization was to provide a databank with fast easy access to enable accurate statistical calculations to be performed on the raw data entries and to simplify t ... | 1983 | 6099643 |
| observations on the stability of foot and mouth disease vaccine antigens. | the 146s particle of the foot and mouth disease virus which is used as a vaccine antigen was found to be relatively stable when stored for prolonged periods at 4 degrees c. however, stored antigens of virus strains of the sat serotypes but not of a virus strain of the type o serotype became less thermostable at 37 degrees c following 4 degrees c storage. vaccines returned from the field 10 months after they were made were shown to contain significant amounts of 146s antigen of the o, a, sat 1 an ... | 1983 | 6099640 |
| purification and immunogenicity of fusion vp1 protein of foot and mouth disease virus. | a procedure has been developed to purify foot and mouth disease virus (fmdv) vp1 surface antigens from recombinant escherichia coli. the vp1 antigens are expressed as fusion proteins derived from the e. coli trp operon and vp1 surface protein of fmdv. the procedure is capable of recovering greater than 96% of the desired product at a purity of greater than 96%. the resulting antigens induce significant levels of virus-neutralizing antibody in guinea pigs and cattle as determined by a mouse prote ... | 1984 | 6099140 |
| reconstitution of immunosuppression mice with mononuclear cells from donors sensitized to foot-and-mouth disease virus (fmdv). | passive transfer experiments were performed to serve as a basis for analyzing the immune response of adult mice to fmdv infection. animals were irradiated (750 rad: 1 lethal dose 50%) and reconstituted with allogeneic mononuclear cells from blood, spleen, thymus and peritoneal cavity from donors 2 and 8 days post-inoculation (p.i.). donors were primed with 10 000 suckling mouse 50% lethal doses of fmdv strain o1 campos. the following parameters were studied in recipient mice challenged with 10 0 ... | 1984 | 6098985 |
| an international collaborative study on foot and mouth disease virus assay methods. 1. virus infectivity and neutralizing antibody assays. | in a collaborative study sponsored by the european commission for the control of foot and mouth disease, workers in 19 laboratories participated in the early phases (1, 2 and 4). all three phases were devoted to assessments of virus infectivity and the neutralizing activity of sera. virus preparations and antisera distributed from one laboratory were tested either by 'in house' or suggested methods. analyses of the results clearly showed that whilst 'within' laboratory variation in the results o ... | 1984 | 6098584 |
| the differentiation of foot and mouth disease virus strains using an indirect sandwich enzyme-linked immunosorbent assay saturation model. | sigmoid saturation curves were fitted to the results of titrations of antiserum to foot and mouth disease virus against homologous and heterologous virus strains. differentiation of strains was readily evident from the different levels of the homologous and heterologous curves. these differences could be quantified by comparison of the saturation curve parameters k and prmax. factors which affect variations in k and prmax and their biological significance were investigated by varying the first p ... | 1984 | 6098582 |
| an indirect sandwich enzyme labelled immunosorbent assay for the detection of foot and mouth disease virus immunizing antigen in tissue culture harvests. | the optimum conditions for an indirect sandwich enzyme-linked immunosorbent assay for foot and mouth disease virus 140s antigen assay are described. factors which could contribute to the variation in the test were investigated and a calibration coefficient for the conversion of elisa values to antigen concentration in micrograms of 140s antigen per millilitre was calculated. antigen mass in nine tissue culture harvests was estimated and these correlated well with estimates made by sucrose densit ... | 1984 | 6098580 |
| nucleotide sequence of the vp1 gene of the foot-and-mouth disease virus strain a venceslau. | the vp1 coat protein of fmdv strain a venceslau (aven) consists of 213 amino acid residues. serum neutralization tests demonstrated that strain aven is closely related to strain a argentina/79 (a79) but significantly different from strain a24cruzeiro (a24). there is a strong correlation between the amino acid sequences and the serological data. nucleotide and amino acid sequence analyses of vp1 showed that serologically related viruses (aven and a79) differ less in this region of the genome than ... | 1984 | 6096217 |
| a rapid enzyme-linked immunosorbent assay for the detection of foot-and-mouth disease virus in epithelial tissues. | a rapid double sandwich enzyme-linked immunosorbent assay (elisa) has been used for the identification and type differentiation of foot-and-mouth disease (fmd) viruses in epithelial tissue samples submitted for diagnosis from the field. no difficulty was experienced in the direct typing of freshly harvested epithelium from recently ruptured vesicles by the complement fixation (cf) test or elisa. the elisa was more sensitive and specific, but proved no more efficient than the traditional cf test ... | 1984 | 6093338 |
| induction of antibody to foot-and-mouth disease virus in presensitized mouse spleen cell cultures. | cultures of spleen cells from immunized mice were stimulated in vitro by soluble preparations of purified foot-and-mouth disease virus. virus-specific antibody, as detected by an enzyme-linked immunosorbent assay, was produced by immune spleen cells but not by normal, nonimmune cells. the optimal specific response was obtained with 1 microgram of virus per ml of culture; as the virus concentration was increased, the production of specific antibody was reduced. for very low concentrations of viru ... | 1984 | 6092687 |
| [effects of temperature and inactivators on strains of foot-and-mouth disease virus in colombia]. | 1984 | 6091209 | |
| the effect of antiserum quality on strain specificity assessment of foot and mouth disease virus by the neutralization reaction. | the factors affecting the virus strain specificity of antibody to foot an mouth disease virus prepared by a variety of protocols in several species were evaluated by neutralization tests. the time at which the serum was taken, the antigen dose given, whether or not revaccination had occurred and the animal species in which the sera were prepared, did not appear to affect the strain specificity of serum prepared to inactivated antigens when measured in neutralization tests, probably because of th ... | 1984 | 6090465 |
| the stability and potency of vaccines prepared from inactivated foot-and-mouth disease virus concentrates. | the stability of 146s particles in concentrates of foot-and-mouth disease virus stored at 4 degrees c was similar to that of 146s particles in a conventional virus preparation. proteolytic degradation of vpl was not observed in the stored conventional virus preparation or inhibitor-supplemented concentrate but was observed in a supplement-free concentrate. the potencies of vaccines made from the conventional and concentrated preparations and stored in parallel at 4 degrees c appeared to decrease ... | 1984 | 6090464 |
| nucleotide sequence and genome organization of foot-and-mouth disease virus. | a continuous 7802 nucleotide sequence spanning the 94% of foot and mouth disease virus rna between the 5'-proximal poly(c) tract and the 3'-terminal poly(a) was obtained from cloned cdna, and the total size of the rna genome was corrected to 8450 nucleotides. a long open reading frame was identified within this sequence starting about 1300 bases from the 5' end of the rna genome and extending to a termination codon 92 bases from its polyadenylated 3' end. the protein sequence of 2332 amino acids ... | 1984 | 6089122 |
| heterogeneity of the polyribocytidylic acid tract in aphthovirus: biochemical and biological studies of viruses carrying polyribocytidylic acid tracts of different lengths. | in this paper we report a study of a sample of foot-and-mouth disease virus carrying two polyribocytidylic acid [poly(c)] tracts of different lengths. by plaque purification in tissue culture, we isolated two populations of particles, one carrying the long poly(c) tract and the other carrying only the short homopolymer. the fingerprints of both viruses were indistinguishable from each other and from that of the virus present in the original sample, suggesting that the main difference between the ... | 1984 | 6088803 |
| inactivation of foot-and-mouth disease virus vaccine strains by activation of virus-associated endonuclease. | a new inactivation process for foot-and-mouth disease virus (fmdv) has been developed. this process is based on the activation of the fmdv endonuclease by incubation of unfractionated viral suspension or purified virions at 37 degrees c in the presence of high concentrations of monovalent cations such as k+, cs+ or nh4+ at ph 8.5. this procedure completely inactivated several fmdv vaccine strains yielding preparations having similar amounts of 140s particles to untreated controls. the inactivati ... | 1984 | 6088682 |
| [electrical properties of the foot-and-mouth disease virus]. | it has been shown that the electron microscopy method can be used for characteristics of the electric properties of foot-and-mouth disease virus. the appearance of simultaneous positive and negative staining during the negative staining of virus preparations with 3-4% ptv solution shows the presence of full virions of constant poles designated as positively and negatively stained areas of protein coat surface. the lateral orientation of virions on the film at routine conditions of preparation an ... | 1984 | 6087939 |
| biotechnological approach to a new foot-and-mouth disease virus vaccine. | major contributions towards the development of an absolutely safe fmdv vaccine are evident. with the identification of vp1 as the immunogenic protein, it is possible to manufacture a subunit vaccine via biotechnology. dna sequences encoding the vp1 protein can be introduced into a bacterium with ease; under the appropriate conditions, large amounts of vp1 can be produced in a short time. the accumulation of amino acid sequences generated by recombinant dna techniques allows identification of ant ... | 1984 | 6085855 |
| location of neutralizing epitopes defined by monoclonal antibodies generated against the outer capsid polypeptide, vp1, of foot-and-mouth disease virus a12. | the epitopes of six monoclonal antibodies generated against type a12 foot-and-mouth disease virus (fmdv) vp1 or its largest cyanogen bromide fragment (13 kd) were characterized. five of these monoclonal antibodies neutralized viral infectivity. solid-phase and competitive antigen binding assays using virion-derived antigens or a biosynthetic vp1 polypeptide identified two distinct neutralizing epitopes. one epitope was located between amino acid residues 145-168 of vp1 and the other between amin ... | 1984 | 6085200 |
| [type variations in the foot-and-mouth disease virus during an epizootic]. | 1966 | 6011615 | |
| further information on the persistence of infective foot-and-mouth disease virus in cattle exposed to virulent virus strains. | 1966 | 5995671 | |
| serological variation of foot-and-mouth disease virus in iran (1963-1966). | 1966 | 5994043 | |
| [experiments with immunization of mice with experimentally increased nonspecific resistance against the foot-and-mouth disease virus]. | 1965 | 5855404 | |
| urine ph changes in cattle infected with foot-and-mouth-disease virus. | 1969 | 5813745 | |
| foot-and-mouth disease virus in semen of bulls and its transmission by artificial insemination. | 1968 | 5680978 | |
| influence of litter size, age, variation, and selective breeding of mice on susceptibility to foot-and-mouth disease virus. | 1968 | 5637929 | |
| [determination of the electrophoretic mobility of the foot-and-mouth disease virus in agar gel by radioactivity measurements]. | 1967 | 5634254 | |
| [the biological activity of mixtures of foot-and-mouth disease virus and serums in animal experiments on mice pretreated unspecifically (cattle spleen extract)]. | 1967 | 5633606 | |
| [thermostabile inhibitor against foot-and-mouth disease virus in pig serum. iv. relationship to antibody production after inoculation]. | 1967 | 5628220 | |
| [carbohydrate metabolism in a culture of embryonic kidney epithelium of swine infected with foot-and-mouth disease virus]. | 1967 | 5600608 | |
| [change in resistance of mice against a viral infection by pretreatment with cyclophosphamide. infection of growing mice with foot-and mouth disease virus]. | 1971 | 5549668 | |
| [identification of types of foot-and-mouth disease virus. nong-saraï, thailand]. | 1970 | 5533933 | |
| typing of the foot-and-mouth disease virus. | 1970 | 5533714 | |
| effect of storage at -20 degrees c on the infectivity of foot-and-mouth disease virus type o. | 1969 | 5400817 | |
| marker studies of foot-and-mouth disease virus. | 1969 | 5400816 | |
| [distribution of the types of foot-and-mouth disease virus in iran during the year 1968]. | 1969 | 5400809 | |
| studies on the carrier state of cattle exposed to foot-and-mouth disease virus. | 1966 | 5219023 | |
| [studies of hand, foot and mouth disease. virus isolation in an epidemic in nobeoka city in 1970]. | 1971 | 4999999 | |
| foot-and-mouth disease in swine. ii. some physical-chemical characteristics of antibodies produced by chemically-treated and non-treated foot-and-mouth disease virus. | 1967 | 4970561 |