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oxygen uptake and local po2 profiles in submerged larvae of phaeoxantha klugii (coleoptera: cicindelidae), as well as their metabolic rate in air.we studied whether oxygen uptake from the surrounding water might enhance survival in submerged third instar larvae of phaeoxantha klugii, a tiger beetle from the central amazonian floodplains. local oxygen partial pressures (po(2)) were measured with microcoaxial needle electrodes close to larvae submerged in initially air-saturated still water. the po(2) profiles showed that the larvae exploit oxygen from the aquatic medium. metabolism in the air of more or less resting larvae was determined b ...201315286912
environment-dependent admixture dynamics in a tiger salamander hybrid zone.after an estimated five million years of independent evolution, the barred tiger salamander (ambystoma tigrinum mavortium) was introduced by bait dealers into the native range of the california tiger salamander (a. californiense). hybridization and backcrossing have been occurring in central california for 50-60 years, or an estimated 15-30 generations. we studied genetic and ecological factors influencing admixture of these two divergent gene pools by analyzing frequencies of hybrid genotypes i ...200415266977
evaluation of attractants and egg-laying substrate preference for oviposition by aedes albopictus (diptera: culicidae).evaluation of oviposition attractants and substrate preferences of aedes albopictus was carried out under laboratory and field conditions. to obtain candidate oviposition substances we used a water rinse of 3 mollusk species: blood cockle (anadara granosa), carpet shell (paphia undulata), and sea mussel (mytilus smaragdinus), and the giant tiger prawn (penaeus monodon). the rinse water of carpet shell and giant tiger prawn showed higher attractiveness for ovipostion than the other candidate attr ...200415266742
quantitative real-time rt-pcr demonstrates that handling stress can lead to rapid increases of gill-associated virus (gav) infection levels in penaeus monodon.gill-associated virus (gav) of the black tiger prawn penaeus monodon has been implicated as a cause of periodic production losses in australia since 1996. we report here the development of a real-time quantitative rt-pcr (qrt-pcr) for gav. a dilution series of in vitro transcribed rna was used to determine the sensitivity limit of the qrt-pcr and as a standard for gav quantification. a linear relationship between cycle threshold (ct) values and input rna was obtained over a wide concentration ra ...200415264715
immuocytochemical analysis of spatial organization of photoreceptors and amacrine and ganglion cells in the tiger salamander retina.in the present study, using double- or triple-label immunocytochemistry in conjunction with confocal microscopy, we aimed to examine the population and distribution of photoreceptors, gabaergic and glycinergic amacrine cells, and ganglion cells, which are basic but important parameters for studying the structure-function relationship of the salamander retina. we found that the outer nuclear layer (onl) contained 82,019 +/- 3203 photoreceptors, of which 52% were rods and 48% were cones. the densi ...201315259567
conservation of wild animals by assisted reproduction and molecular marker technology.wild animals are an integral component of the ecosystem. their decimation due to abrupt natural calamities or due to gradual human intervention would be disastrous to the ecosystem and would alter the balance in nature between various biotic components. such an imbalance could have an adverse effect on the ecosystem. therefore, there is an urgent need to put an end to the ever increasing list of endangered species by undertaking both in situ and ex situ conservation using tools of modern biology ...200315255374
the in vitro neuromuscular activity of indo-pacific sea-snake venoms: efficacy of two commercially available antivenoms.we examined the neurotoxicity of the following sea snake venoms: enhydrina schistosa (geographical variants from weipa and malaysia), lapemis curtus (weipa and malaysia), laticauda colubrina, aipysurus laevis, aipysurus fuscus and aipysurus foliosquamatus. venom from a terrestrial snake, notechis scutatus (tiger snake), was used as a reference. all venoms (1 and 3 microg/ml) abolished indirect twitches of the chick biventer cervicis muscle and significantly inhibited responses to ach (1 mm) and ...200415246769
expression of calcium transporters in the retina of the tiger salamander (ambystoma tigrinum).changes in intracellular calcium concentration, [ca2+]i, modulate the flow of visual signals across all stages of processing in the retina, yet the identities of ca2+ transporters responsible for these changes are still largely unknown. in the current study, the distribution of plasma membrane and intracellular ca2+ transporters in the retina of tiger salamander, a model system for physiological studies of retinal function, was determined. plasma membrane calcium atpases (pmcas), responsible for ...200415236230
simultaneous strain and temperature measurements with polarization-maintaining fibers and their error analysis by use of a distributed brillouin loss system.simultaneous temperature and strain measurement with a distributed brillouin loss system is proposed by use of the parameters brillouin frequency, power, and bandwidth, for panda, bow-tie, and tiger polarization-maintaining fibers for the first time to our knowledge. the expressions for simultaneous temperature and strain sensing and the maximum errors and rms values of temperature and strain measurements are derived with three combinations of the parameters: (1) power and brillouin frequency, ( ...200415233429
trichothiodystrophy: quantification of cysteine in human hair and nails by application of sodium azide-dependent oxidation to cysteic acid.the term "trichothiodystrophy" (ttd) covers several autosomal recessive diseases whose diagnostic hallmark is short, brittle hair low in sulfur and cystine because of impaired synthesis of high-sulfur matrix protein. clinical symptoms associated with ttd represent a variable range of abnormalities in organs derived from ectoderm and neuroectoderm. important laboratory tests of the hair for the diagnosis of ttd comprise polarizing microscopy ("tiger-tail" pattern), electron microscopy, and amino ...200415232704
evaluation of pseudomonas sp. pm 11 and vibrio fluvialis pm 17 on immune indices of tiger shrimp, penaeus monodon.occurrence of widespread epizootics among cultured stock of shrimp has put research programmes on preventive approaches such as application of probiotics on a high priority in aquaculture. in the present study two bacteria, pseudomonas sp. pm 11 and vibrio fluvialis pm 17 were selected as candidate probionts from a pool of bacteria isolated from gut of farm reared sub-adult shrimp and tested for their effect on the immunity indicators of tiger shrimp. sub-adult shrimp, weighing 14 to 22 g were t ...200415212731
mental health: asia's tigers get the blues. 200415201883
a case of nutritional secondary hyperparathyroidism in a siberian tiger cub.a three-month-old female siberian tiger cub with hindlimb ataxia was referred to the veterinary teaching hospital of konkuk university. the patient was fed only beef without supplementation of calcium and vitamins after weaning. the tiger was presented with ataxia and back pain on digital palpation. in addition, abnormal gait, reluctance to move, and depressed withdrawal reflex were noted at the neurological examination. the overall osteodystrophic change of the lumbosacral vertebrae was observe ...200415187368
olfactory receptor gene expression in tiger salamander olfactory epithelium.physiological studies of odor-elicited responses from the olfactory epithelium and bulb in the tiger salamander, ambystoma tigrinum, have elucidated a number of features of olfactory coding that appear to be conserved across several vertebrate species. this animal model has provided an accessible in vivo system for observing individual and ensemble olfactory responses to odorant stimulation using biochemical, neurophysiological, and behavioral assays. in this paper we have complemented these stu ...200415174087
desensitization of the mglur6 transduction current in tiger salamander on bipolar cells.light depolarizes retinal on bipolar cells, opening the cation-selective channels that are responsible for producing the synaptic current. in this study, the basic features of light-induced signals were mimicked by bathing slices of salamander retina with an agonist for the mglur6 receptor that is expressed on the dendrites of on cells, and then displacing the agonist with the mglur6 antagonist (rs)-a-cyclopropyl-4-phosphonophenylglycine (cppg). the transduction current that is activated by this ...200415146044
molecular cloning and characterisation of a cell adhesion molecule, peroxinectin from the white shrimp litopenaeus vannamei.expression of peroxinectin cdna was obtained from haemocytes of white shrimp litopenaeus vannamei using oligonucleotide primers based on the peroxinectin sequence of tiger shrimp penaeus monodon and freshwater crayfish pacifastacus leniusculus. the peroxinectin of l. vannamei was constitutively expressed. the cloning of peroxinectin cdna fragment was subjected to rt-pcr using degenerated primers. both 3'- and 5'-regions were isolated by rapid amplification of cdna end race method. analysis of nu ...200415145414
a new sensitive isotropic-anisotropic separation experiment-speed mas.a new sensitive 2d isotropic-anisotropic separation experiment that utilizes stroboscopic phase encoding in the evolution dimension (speed) under magic angle sample spinning is presented. this 2d experiment consists of a train of 2n - 1 pi pulses that are applied over 2n rotor periods. the pi pulse train effectively reduces the apparent spinning speed in the evolution dimension by a factor of 1 / (2n) from the mechanical spinning speed. thus, problems commonly associated with magic angle turning ...200415140441
fitness advantage and cytoplasmic incompatibility in wolbachia single- and superinfected aedes albopictus.wolbachia are obligate, maternally inherited, intracellular bacteria that infect numerous insects and other invertebrates. wolbachia infections have evolved multiple mechanisms to manipulate host reproduction and facilitate invasion of naive host populations. one such mechanism is cytoplasmic incompatibility (ci) that occurs in many insect species, including aedes albopictus (asian tiger mosquito). the multiple wolbachia infections that occur naturally in a. albopictus make this mosquito a usefu ...200415127087
a new species of copepod (siphonostomatoida: caligidae) parasitic on the tiger shark galeocerdo cuvier (péron & lesueur) from western australian waters.a new species of sea-louse (caligidae, siphonostomatoida), caligus oculicola n. sp., is described from the eye surface of the tiger shark galeocerdo cuvier from off the northwestern coast of australia. this copepod is distinguished from its congeners by a combination of characters that include: (i) a bifid, dentiform process of the maxillule; (ii) a sternal furca with a box longer than wide and diverging, truncate tines; (iii) terminal spines 1 to 3 on the last segment of leg 1 exopod, each with ...200415084834
microsatellite dna markers: evaluating their potential for estimating the proportion of hatchery-reared offspring in a stock enhancement programme.we describe a statistical method for estimating the effectiveness of a stock enhancement programme using nuclear dna loci. it is based on knowing the population allele frequencies and the genotypes of the hatchery parents (mother only, or mother and father), and on determining the probability that a wild-born animal will by chance have a genotype consistent with hatchery origin. we show how to estimate the proportion of released animals in the wild population, and its standard error. the method ...200415078464
taming the tigers: the case for controlling the tobacco market. 200415078226
establishment of enteral nutrition: prokinetic agents and small bowel feeding tubes.nutritional support is vital to improving the clinical outcomes in patients in the intensive care unit. enteral nutrition should be administered early and aggressively, thereby reducing the need for parenteral nutrition. because nasogastric feeding is often associated with gastrointestinal intolerance, recent research has focused on the use of prokinetic agents or small bowel feeding tubes to enhance the successful establishment and maintenance of enteral nutrition.200415075727
morphological and physiological identification of medulla interneurons in the visual system of the tiger beetle larva.the morphology of visual interneurons in the tiger beetle larva was identified after recording their responses. stained neurons were designated as either medulla or protocerebral neurons according to the location of their cell bodies. medulla neurons were further subdivided into three groups. afferent medulla neurons extended processes distally in the medulla neuropil and a single axon to the brain through the optic nerve. they received their main input from stemmata on the ipsilateral side. two ...200415069573
a novel microsatellite (str) marker for forensic identification of big cats in india.india is the home to five of the eight majestic big cats of the world. the three major big cats namely, lion, tiger, and leopard are listed in the schedule i of the indian wildlife protection act, 1972. apart from the severe loss of the habitat, these are continuously facing the danger of extinction mainly due to poaching and hunting for their body parts, which are being greatly valued by apothecaries marketing traditional chinese medicines. with the advent of polymerase chain reaction (pcr), dn ...200415062954
interspecific androgenetic restoration of rosy barb using cadaveric sperm.interspecific androgenetic rosy barb (puntius conchonius) was generated using its cadaveric (-20 degrees c) or fresh sperm to activate nuclear genome inactivated oocytes of gray tiger barb (puntius tetrazona). uv irradiation was used to inactivate nuclear genome of tiger barb oocytes. thermal shock restored diploidy of rosy barb in the oocytes of tiger barb. survival of androgenotes was 14% or 7% when fresh or cadaveric sperm was used. the diploid or haploid nuclear genome of rosy barb, individu ...200415060603
turning a tiger into a house cat: using legionella pneumophila to study coxiella burnetii. 200415058276
tigers and their prey: predicting carnivore densities from prey abundance.the goal of ecology is to understand interactions that determine the distribution and abundance of organisms. in principle, ecologists should be able to identify a small number of limiting resources for a species of interest, estimate densities of these resources at different locations across the landscape, and then use these estimates to predict the density of the focal species at these locations. in practice, however, development of functional relationships between abundances of species and th ...200415041746
bite marks--the criminal's calling cards.carnivorous animals, like dogs or tigers, use their teeth in two distinct ways. they kill their prey primarily using their canines and they tear and slice the flesh to produce digestible fragments. human teeth are designed principally to cut and grind food which is usually previously prepared. some people appear to revert to more primitive instincts and use their canines and incisors to inflict bites on unsuspecting victims.200415039746
an application of visible human database in radiotherapy: tutorial for image guided external radiotherapy (tiger).three-dimensional conformal radiotherapy and intensity modulated radiotherapy allow accurate dose delivery on target volumes. due to the different background among specialists involved in target volume definition, the contouring emerges as one of the most questionable steps in treatment planning procedures. a software tool devoted to contouring training, named tutorial for image guided external radiotherapy ('tiger'), based on the visible human project images data-set, is described.200415028403
big cat scan: magnetic resonance imaging of the tiger.in august 2002, we performed mri scans on a female juvenile bengal tiger. we present the clinical course, imaging and autopsy findings, and some comparative anatomy of the tiger brain and skull. magnetic resonance images of a tiger have not previously been published.200415027932
an extremely sensitive species-specific arms pcr test for the presence of tiger bone dna.the survival of the tiger (panthera tigris) is seriously threatened by poaching to provide raw materials for traditional chinese medicines (tcms). most highly prized are the tiger's bones, which are used in combination with other animal and plant derivatives in pills and plasters for the treatment of rheumatism and other ailments. hundreds of patent remedies have been produced which claim to contain tiger bone, but proof of its presence is needed, if legislation prohibiting the trade in endanger ...200415017992
gingival neoplasms in a captive sand tiger shark, carcharias taurus (rafinesque), and a wild-caught blue shark, prionace glauca (l.). 200415009246
wild cat bite--a narrow escape.an unusual case is reported of a tiger bite and trauma to the maxillofacial region of a 45-year-old male patient. wild animal assaults on the general population are on the increase in india and it is possible that further such cases will occur. the increased attacks could be a result of massive deforestation, resulting in disturbance to the ecosystem and tampering of the natural habitat of the royal cat.200415005474
climate change. a eurasian tiger threatens to maul kyoto. 200415001755
delayed myotoxicity in snake envenoming by the tiger snake group.snakebite on the north coast of new south wales is not an uncommon cause of envenoming. we report a case of a young woman who presented after a snake bite and did not develop fulminant signs and symptoms of envenoming until 32 h after the bite.200514992067
activation of glutamate transporters in rods inhibits presynaptic calcium currents.we found that l-glutamate (l-glu) inhibits l-type ca2+ currents (ica) in rod photoreceptors. this inhibition was studied in isolated rods or rods in retinal slices from tiger salamander using perforated patch whole cell recordings and cl(-)-imaging techniques. application of l-glu inhibited ica by approximately 20% at 0.1 mm and approximately 35% at 1 mm. l-glu also produced an inward current that reversed around ecl. the metabotropic glutamate receptor (mglur) agonists t-ada (group i), dcg-iv ( ...201614977334
senior care: making a difference in long-term care of older adults.this article describes the creation of senior care, a practice of the university of missouri sinclair school of nursing (musson). senior care is a home care agency that specializes in care of frail older adults. grant funds assisted senior care in start up, and the program generated more than 1.25 million dollars in service revenue during the past fiscal year. more than 300 students, from nursing and other schools across the university, have used senior care as a clinical or service-learning sit ...200414974516
surgical extraction of a fractured, non-vital deciduous tooth in a tiger.a 10-month-old, female/intact tiger (panthra tigris) was presented for a fractured deciduous mandibular right canine tooth, mandibular swelling, and a cutaneous draining tract ventral to the apex of the fractured tooth. the tooth had chronic pulpal exposure and was considered non-vital with periapical osteomyelitis. this case report describes treatment to optimize normal development and eruption of the permanent mandibular right canine tooth.200314974169
cloning success marks asian nations as scientific tigers. 200414973443
access to information about harm and safety in spider fearful and nonfearful individuals: when they were good they were very very good but when they were bad they were horrid.this study tests two alternative hypotheses about how phobic information is processed in spider fearful and nonfearful individuals: (1) the threat-related cognitive set hypothesis and (2) the dimensions hypothesis. counter to the traditional cognitive model of fear, the dimensions hypothesis predicts that spider fearful individuals tend to prioritise the harm-safety dimension when evaluating animal stimuli, and the consequent stretching of that evaluative dimension will confer advantage to the a ...200314972673
talking to a tiger: fathers reveal their difficulties in communicating about sexuality with adolescents. 200214964944
genetic heterogeneity of the giant tiger shrimp (penaeus monodon) in thailand revealed by rapd and mitochondrial dna rflp analyses.genetic diversity of the giant tiger shrimp (penaeus monodon) collected from 5 areas, chumphon and trat (gulf of thailand), and phangnga, satun, and trang (andaman sea), was examined by randomly amplified polymorphic dna (rapd) and mitochondrial dna (16s ribosomal dna and an intergenic coi-coii) polymorphism. a total of 53 polymorphic fragments from ubc299, ubc273, and ubc268 was consistently scored across all samples. from the respective primers 26, 32, and 30 genotypes were generated. a 260-bp ...200114961335
development of vitellogenin-elisa, an in vivo bioassay, and identification of two vitellogenesis-inhibiting hormones of the tiger shrimp penaeus monodon.a sandwich enzyme-linked immunosorbent assay (elisa) for the quantification of vitellogenin in the hemolymph of penaeus monodon is reported. lipovitellin from the mature ovary was purified using hydroxylapatite column chromatography and used as the standard protein, which was serially diluted from 3336 to 6.51 microg and gave a linear plot. sensitivity results showed elisa was insensitive to samples that did not contain vitellogenin or lipovitellin. specificity results showed the degree of the d ...200114961329
identification of two distinct molt-inhibiting hormone-related peptides from the giant tiger prawn penaeus monodon.six peptides belonging to the crustacean hyperglycemic hormone (chh) family were isolated from extracts of the sinus glands of the giant tiger prawn penaeus monodon by reverse-phase high-performance liquid chromatography. these were designated pem-sgp-a to pem-sga-f (pem, penaeus monodon; sgp, sinus gland peptide) and their amino-terminal amino acid sequences were analyzed. five of the 6 peptides (pem-sgp-a, -b, -d, -e, and -f) exhibited similar amino acid sequences to those of chh peptides that ...200214961272
identification of immune-related genes in hemocytes of black tiger shrimp (penaeus monodon).an expressed sequence tag (est) library was constructed from hemocytes of the black tiger shrimp (penaeus monodon) to identify genes associated with immunity in this economically important species. the number of complementary dna clones in the constructed library was approximately 4 x 10(5). of these, 615 clones having inserts larger than 500 bp were unidirectionally sequenced and analyzed by homology searches against data in genbank. significant homology to known genes was found in 314 (51%) of ...200214961242
[on o-tricresylphosphate poisoning in tigers]. 195214949240
introducing the marine tiger. 195214941942
a rare displacement of a canine in a tiger. 195114808229
two instances of proved rabies in the tiger. 195014794203
connexin35/36 gap junction proteins are expressed in photoreceptors of the tiger salamander retina.photoreceptors in the vertebrate retina are electrically coupled with one another. such coupling plays important roles in visual information processing. physiological properties of rod-rod and rod-cone coupling have been best studied in the salamander retina, yet the cellular and molecular basis of these electrical synapses has not been established. recently, connexin35/36 (cx35/36) gap junction proteins were found to be highly expressed in brain and retina, suggesting that it may mediate photor ...200414755521
neural impacts on the regeneration of skeletal muscles.the regeneration of skeletal muscles is a suitable model to study the development and differentiation of contractile tissues. neural effects are one of the key factors in the regulation of this process. in the present work, effects of different reinnervation protocols (suture or grafting) were studied upon the regenerative capacity of rat soleus muscles treated with the venom of the australian tiger snake, notexin, which is known to induce complete necrosis and subsequent regeneration of muscles ...200314740009
serological relationships among genotypic variants of betanodavirus.betanodaviruses, the causative agents of viral nervous necrosis or viral encephalopathy and retinopathy, are divided into 4 genotypes based on the coat protein gene (rna2). in the present study, serological relationships among betanodavirus genotypic variants were examined by virus neutralization tests using rabbit antisera raised against purified virions of strains representative of each genotype. all 20 isolates examined shared epitopes for neutralizing, but they fell into 3 major serotypes (a ...200314735917
identification of gyrkppfngsifamide (crustacean-sifamide) in the crayfish procambarus clarkii by topological mass spectrometry analysis.a new concept relating to the purification protocol for biological proteins and peptides has been designed as "topological mass spectrometry analysis," in combination with maldi-tof ms using slices of tissues, chromatographic purification from the extract of tissues, molecular cloning for the determination of the precursor structure, and capillary lc-ms/ms analysis for elucidation of its posttranslational modifications. in an actual application, we identified an alpha-amidated neuropeptide from ...200414723891
temperature dependence of brillouin frequency, power, and bandwidth in panda, bow-tie, and tiger polarization-maintaining fibers.we report a study of the temperature dependence of the brillouin gain and loss for three different kinds of commercial polarization-maintaining fibers for the first time to our knowledge. the brillouin frequency differences between the fast and slow axes are independent of the temperature, varying between 2.9 and 4.3 mhz. using 2-ns pulses (equivalent to a spatial resolution of 20 cm), we find that the temperature coefficients for the relative brillouin power at a wavelength of 1310 nm are 0.26% ...200414719646
expression of biologically active crustacean hyperglycemic hormone (chh) of penaeus monodon in pichia pastoris.crustacean hyperglycemic hormone (chh), molt-inhibiting hormone (mih), and gonad-inhibiting hormone (gih) are members of a major peptide family produced from the x-organ sinus gland complex in the eyestalk of crustaceans. this peptide family plays important roles in controlling several physiologic processes such as regulation of growth and reproduction. in this study the complementary dna encoding a peptide related to the chh/mih/gih family (so-called pem-cmg) of the black tiger prawn penaeus mo ...200314719165
pulmonary tuberculosis due to mycobacterium bovis subsp. caprae in captive siberian tiger.we report the first case of pulmonary tuberculosis caused by mycobacterium bovis subsp. caprae in a captive siberian tiger, an endangered feline. the pathogen was isolated from a tracheal aspirate obtained by bronchoscopy. this procedure provided a reliable in vivo diagnostic method in conjunction with conventional and molecular tests for the detection of mycobacteria.200314718093
trimetazidine in geriatric patients with stable angina pectoris: the tiger study.this study aims to assess the efficacy and tolerance of the metabolic antianginal agent trimetazidine, a 3-kat inhibitor, in 141 stable angina patients aged 65-86 years. efficacy was assessed with exercise tests and clinical evaluation after 12 weeks of treatment. the main outcome was an increase in exercise duration by 52 +/- 92 sec (p < 0.001). other exercise test parameters also improved, with no change in rate-pressure product. angina attacks and short-acting nitrate consumption significantl ...200314712887
has aedes albopictus established in california?significant numbers of the asian tiger mosquito, aedes albopictus, were detected on the west coast of the usa in mid-june 2001, in containerized oceanic shipments of "lucky bamboo" (dracaena spp.) originating from south china. wholesale nurseries in california importing large quantities of lucky bamboo became the focal points of infestation. greater los angeles county vector control district immediately implemented an adulticiding protocol at the los angeles/long beach harbors, followed by larvi ...200314710729
immunolocalization of allatostatin-like neuropeptides and their putative receptor in eyestalks of the tiger prawn, penaeus monodon.allatostatin (ast)-like immunoreactivity (ir) was localized in the eyestalk of penaeus monodon by immunohistochemistry using four anti-ast antibodies. depending on the antisera, ast-like immunoreactivity was detected in neuronal bodies of the lamina ganglionalis, cell bodies anterior to the medulla externa and cell bodies on the anterior and posterior of the medulla terminalis. neuronal processes in neuropiles of the medulla externa, medulla terminalis, sinus gland and nerve fibers in the optic ...200314706535
endodontic treatment of a tiger. 200314705433
nasalance measures in german-speaking cleft patients.the purpose of this study was to evaluate nasalance measures in german-speaking patients with different types of repaired cleft lip and palate and to find out if significant nasalance gender differences exist in the different cleft groups. a total of 125 german-speaking cleft patients (74 male and 51 female) were included in this study: 18 patients with isolated unilateral cleft lip (ucl; mean age: 13.00 +/- 2.03 years), 66 patients with complete unilateral cleft lip and palate (uclp; mean age: ...200414704583
[changes of interleukin-6 and janus kinases in rats with hypoxic pulmonary hypertension].to investigate the expressions of interleukin 6 (il-6) and janus kinases (jaks) in rats with hypoxia induced pulmonary hypertension (hph).200314703438
helicobacter acinonychis: genetic and rodent infection studies of a helicobacter pylori-like gastric pathogen of cheetahs and other big cats.insights into bacterium-host interactions and genome evolution can emerge from comparisons among related species. here we studied helicobacter acinonychis (formerly h. acinonyx), a species closely related to the human gastric pathogen helicobacter pylori. two groups of strains were identified by randomly amplified polymorphic dna fingerprinting and gene sequencing: one group from six cheetahs in a u.s. zoo and two lions in a european circus, and the other group from a tiger and a lion-tiger hybr ...200414702304
crouching tiger, hidden dragon: the laboratory diagnosis of severe acute respiratory syndrome. 200414699466
molecular cloning and characterization of tiger shrimp (penaeus monodon) transglutaminase.transglutaminases (tg) are important for blood coagulation and post-translation remodeling of proteins. using a plaque screening assay, we isolated cdna encoding a novel tg from a shrimp (penaeus monodon) hemocyte cdna library. the tg cdna consists of 2988 bp with an open reading frame of 2271 bp. the deduced protein has 757 amino acid residues, a calculated molecular mass of 84,713 da and an isoelectric point of 5.56. neither a typical hydrophobic leader sequence nor a transmembrane domain coul ...200414698215
implementation strategies for a scottish national epilepsy guideline in primary care: results of the tayside implementation of guidelines in epilepsy randomized (tiger) trial.to determine the effectiveness of two dissemination and implementation strategies to implement a national guideline for epilepsy management in primary care settings.200414692904
local people's attitudes towards conservation and wildlife tourism around sariska tiger reserve, india.conservationists in the recent years view local peoples' support for protected areas management as an important element of biodiversity conservation. this is often linked to the direct benefits, which local communities get from the protected areas. these benefits could be in the form of biomass resources, park funds diverted to local villages by state agencies and revenue from wildlife tourism. there are a very few studies which have attempted to study the direct relationship between benefits fr ...200314680895
establishment of proliferative cell nuclear antigen gene as an internal reference gene for polymerase chain reaction of a wide range of archival and fresh mammalian tissues.polymerase chain reaction (pcr) from paraffin-embedded tissues provides a powerful tool to amplify dna from a variety of recent and archival material. because dna from paraffin-embedded samples is more degraded than from fresh material, the amplification of reference genes is essential to exclude false-negative results. this study describes the use of the proliferative cell nuclear antigen (pcna) gene as a reference gene in a range of animal species and in humans. the pcna-pcr to amplify a fragm ...200314667025
culture and phenotypic characterization of a wolbachia pipientis isolate.the recent isolation of wolbachia pipientis in the continuous cell line aa23, established from eggs of a strain of the asian tiger mosquito aedes albopictus, allowed us to perform extensive characterization of the isolate. bacterial growth could be obtained in c6/36, another a. albopictus cell line, at 28 degrees c and in a human embryonic lung fibroblast monolayer at 28 and 37 degrees c, confirming that its host cell range is broader than was initially thought. the bacteria were best visualized ...200314662922
how the mesencephalic locomotor region recruits hindbrain neurons.this chapter summarizes experiments which were designed to reveal how repetitive electrical stimulation of the mesencephalic locomotor region (mlr) recruits nearby hindbrain neurons into activity, such that locomotion can ensue in the tiger salamander, a. tigrinum. the mlr stimulus strength was subthreshold or near-threshold for locomotor movements to ensue. such relatively weak stimulation of the mlr produced locomotor movements after a relatively long delay, which featured neuronal interaction ...200414653167
pure akinesia: an unusual phenotype of hallervorden-spatz syndrome.two siblings were seen in our outpatient movement disorders clinic with an indolent clinical picture of a pure akinesia syndrome. magnetic resonance showed the typical "eye of the tiger" sign, and genetic screening disclosed that both siblings were compound heterozygotes with two missense mutations in the pank2 gene 734a-->g and 1172t-->c. this case report highlights the phenotypic diversity of hallervorden spatz syndrome and the need for further investigation of adult-onset pure akinesia syndro ...200314639680
[enhanced expression of signal transducers and activators of transcription in lung tissue of hypoxic pulmonary hypertension rat models].to investigate the expression levels of signal transducers and activators of transcription (stats) in the lung tissue of hypoxic pulmonary hypertension (hph) rat models.200314636463
photostimulated luminescence detection of irradiated shellfish: international interlaboratory trial.an interlaboratory trial was conducted to validate photostimulated luminescence (psl) detection of irradiated shellfish. five species of shellfish (nephrops norvegicus, mussels, black tiger prawns, brown shrimps, and king scallops) were presented blind as nonirradiated and irradiated to 0.5 and 2.5 kgy. precharacterization analysis of each product and treatment was performed on both whole (including shell) and intestinal samples. the results for whole samples (including shell) confirmed that the ...200814632401
thermoluminescence detection of irradiated shellfish: international interlaboratory trial.an international interlaboratory trial was conducted using thermoluminescence for the detection of irradiated shellfish, aimed at validating the method for routine use. nephrops norvegicus, mussels, brown shrimps, black tiger prawns, and king scallops were presented as nonirradiated and irradiated to 0.5 and 2.5 kgy. the protocol called for the use of 3 preparation methods: extraction of silicates from whole shellfish by acid hydrolysis and physical separation, and of carbonates from powdered sh ...200814632400
unraveling the hallervorden-spatz syndrome: pantothenate kinase-associated neurodegeneration is the name.after the recent discovery of the major genetic defect in neurodegeneration with brain iron accumulation (nbia, formerly hallervorden-spatz syndrome), this heterogeneous group of disorders can now be differentiated by clinical, radiographic, and molecular features.200314631201
drug resistance in nematodes: a paper tiger or a real problem?the purpose of this review is to illustrate where drug resistance in parasitic nematodes has become a major problem. the mechanisms underlying anthelmintic resistance, the possible reasons for the development of anthelmintic resistance, and recommendations to minimize the further development of anthelmintic resistance in humans will be addressed.200314624106
aging in a tiger welfare regime: the single elderly in hong kong.this paper explores the coping strategies of the low income single elderly in hong kong, which are divided into several broad types: government-orientated (public services); market-orientated; community-orientated; and individually-orientated (practically speaking self restraint and denial). it highlights this group's reliance on the government, which not only provides housing, medical care and other services, but is also a major source of income support, although support levels are seriously in ...200114617983
genomic sequence of a ranavirus (family iridoviridae) associated with salamander mortalities in north america.disease is among the suspected causes of amphibian population declines, and an iridovirus and a chytrid fungus are the primary pathogens associated with amphibian mortalities. ambystoma tigrinum virus (atv) and a closely related strain, regina ranavirus (rrv), are implicated in salamander die-offs in arizona and canada, respectively. we report the complete sequence of the atv genome and partial sequence of the rrv genome. sequence analysis of the atv/rrv genomes showed marked similarity to other ...200314599794
the hyperpriming phenomenon in normal aging: a consequence of cognitive slowing?increased semantic priming effects (hyperpriming) are sometimes observed in alzheimer's disease (ad) and in normal aging. whereas the processes underlying this phenomenon are now well understood in ad, the interpretation is much more woolly in normal aging. to explore semantic priming, the authors used a lexical decision task in which the influence of attention and cognitive slowing was controlled. to explore the semantic organization, the words had coordinate (tiger-lion) or attribute relations ...200314599272
never trust a tiger. 200314584335
anesthetic induction of captive tigers (panthera tigris) using a medetomidine-ketamine combination.six adult female tigers (panthera tigris) were anesthetized repeatedly for elective medical procedures using 3 mg medetomidine and 200 mg ketamine i.m. inductions were rapid and smooth, although supplemental ketamine was needed for safe transport after induction in 6 of 17 procedures. reversal of the medetomidine-induced sedation with 15 mg atipamezole i.m. 59-232 min after induction resulted in smooth, rapid recoveries.200314582797
blastomycosis in nondomestic felids.blastomycosis was diagnosed in six nondomestic felids from eastern tennessee, including two asian lions (panthera leo persicus), one african lion (panthera leo), one siberian tiger (panthera tigris), one cheetah (acinonyx jubatus), and one snow leopard (panthera uncia). clinical signs included lethargy, anorexia, weight loss, dyspnea, sneezing. ataxia, and paresis. variable nonspecific changes included leukocytosis, monocytosis, moderate left shift of neutrophils, moderate hypercalcemia, hyperpr ...200314582783
familial pediatric rapidly progressive extrapyramidal syndrome: is it hallervorden-spatz disease?the clinical features of two children of a family with rapidly progressive extrapyramidal-pyramidal-dementia complex have been described. inheritance seems most likely to be autosomal recessive. magnetic resonance imaging results of brain were negative. even so, the authors argued in favor of a diagnosis of hallervorden-spatz disease because the cases fulfilled the clinical criteria for diagnosis of this disease. apart from the negative magnetic resonance findings, the other unusual feature was ...200314580665
n-methyl-d-aspartate receptors contribute to the baseline noise of retinal ganglion cells.whole-cell recordings of tiger salamander ganglion cells were obtained using a superfused retinal slice preparation. membrane current fluctuations were recorded under voltage-clamp conditions with cells usually held at -70 mv. current fluctuations at rest (mg2+ = 1 mm) were reduced by adding d-2-amino-7-phosphonoheptanoate (d-ap7). resting fluctuations were increased by adding n-methyl-d-aspartate (nmda) or by removing extracellular mg2+. these increased fluctuations were blocked by d-ap7. block ...201414570254
diagnostic and molecular evaluation of three iridovirus-associated salamander mortality events.in 1998 viruses were isolated from tiger salamander larvae (ambystoma tigrinum diaboli and a. tigrinum melanostictum) involved in north dakota and utah (usa) mortality events and spotted salamander (a. maculatum) larvae in a third event in maine (usa). although sympatric caudates and anurans were present at all three sites only ambystomid larvae appeared to be affected. mortality at the north dakota site was in the thousands while at the utah and maine sites mortality was in the hundreds. sick l ...200314567216
was elusive carnivore a panther? dna typing of faeces reveals the mystery.in this study, we report the findings of a recent case in which the officials of an indian zoo claimed that an animal, possibly a carnivore, is periodically visiting the zoo from a nearby vast forest area and causing panic in zoo and nearby villages. they collected some elusive faecal material from the vicinity of an herbivore enclosure. looking to the pugmarks found in that area and faecal matter ceased, the officials could not decide whether it was a lioness, a tiger or a panther. we resolved ...200314550608
weights and linear measurements of the body and of some organs of the tiger salamander. 196114462679
animal fats. 6. the component acids of tiger fat and of puma fat. 195514363118
[microsporosis in 2 tigers]. 196514298543
sleeping tiger: do not disturb. 196414267857
[neurology and neuropathology of tigers in captivity. 1. on a conclusive encephalopathy following a generalized vascular disorder resembling periarteritis nodosa]. 196314198892
[neurology and neuropathology of tigers in captivity. 2. subacute meningoencephalitis with a picture of epileptic confusion and unusual vascular changes]. 196414191003
weights and linear measurements of the body and organs of the tiger salamander, before and after metamorphosis, compared with the adult. 196414123498
a pharmacologically active choline ester and other substances in the garden tiger moth, arctia caja (l.). 196013849067
active immunization of man against the venom of the australian tiger snake (notechis scutatus). 196013844637
venom yields and toxicity of the venoms of male and female tiger snakes. 196013785113
anaphylactic shock and subsequent dementia following the administration of tiger-snake antivenene. 196013774388
tiger meets man. 196113726768
pantothenate kinase-associated neurodegeneration: mr imaging, proton mr spectroscopy, and diffusion mr imaging findings.we herein report the case of a 15-year-old male patient with pantothenate kinase-associated neurodegeneration. the classic "eye-of-the-tiger" appearance was initially present on the globus pallidi on t2-weighted mr images and had disappeared by the time of the 10-month follow-up examination. fluid-attenuated inversion recovery images revealed marked hypointensity in the globus pallidi and dentate nuclei and high signal intensity changes in the deep cerebral white matter. proton mr spectroscopy r ...200313679293
the separation and properties of the neurotoxins from the venom of the tiger snake notechis scutatus scutatus. 195813607406
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