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animal model of noninvasive entamoeba histolytica infection. 200011070301
entamoeba histolytica: localization of the gal/galnac adherence lectin in experimental amebic liver abscess. 200011070300
does the light subunit of the gal/galnac specific lectin have a role in the virulence of entamoeba histolytica? 200011070299
l6: a proteinase- and phagocytosis-deficient mutant of entamoeba histolytica. 200011070298
binding of the galactose-specific lectin of entamoeba histolytica to hamster hepatocyte primary cultures. 200011070296
role of the entamoeba histolytica gal/galnac lectin in recruiting proteins to the host:parasite interface. 200011070295
role of excretory-secretory products of entamoeba histolytica in human amebiasis. 200011070294
entamoeba histolytica cysteine proteinases degrade human colonic mucin and alter its function. 200011070293
novel thermo-stable serine-metallo proteinase of entamoeba histolytica. 200011070292
disturbance of tight junctions by entamoeba histolytica: resistant vertebrate cell types and incompetent trophozoites. 200011070291
role of host caspases in cell killing by entamoeba histolytica. 200011070290
pathogenesis of entamoeba histolytica depends on the concerted action of numerous virulence factors. 200011070289
entamoeba histolytica is unable to use free cholesterol, phospholipids, and fatty acids under axenic cultivation conditions. 200011070288
centromeric structure identification in entamoeba histolytica by anticentromeric/kinetochore antibodies obtained from patients with the crest syndrome. 200011070286
structural organization of gamma-tubulin in the microtubule organizing center (mtoc) during the nuclear division of entamoeba histolytica trophozoites. 200011070285
the chromosomes of entamoeba histolytica. 200011070284
identification of seven chemical factors that favor high-quality entamoeba histolytica cyst-like structure formation under axenic conditions. 200011070279
subcellular distribution and in situ localization of the acid phosphatase of entamoeba histolytica. 200011070275
digestion of erythrocytes by entamoeba histolytica. correlative study with scanning electron microscopy and polyacrylamide gel electrophoresis. 200011070274
cellular and biochemical analysis of phagocytosis in entamoeba histolytica. 200011070273
a mitochondrial-type hsp70 gene of entamoeba histolytica. 200011070272
molecular cloning of a gene encoding a pdi-like protein from entamoeba histolytica. 200011070271
characterization of the ehrabb recombinant protein of entamoeba histolytica. 200011070270
entamoeba histolytica codes for a protein homologue of the sec61 alpha subunit, a component of the endoplasmic reticulum translocon. 200011070269
ultrastructural evidence of smooth endoplasmic reticulum and golgi-like elements in entamoeba histolytica and entamoeba dispar. 200011070268
cloning of the entamoeba histolytica stt3 gene, a subunit of the oligosaccharyltransferase complex. 200011070267
entamoeba histolytica contains a ter-atpase. 200011070266
rab8 as a molecular model of vesicular trafficking to investigate the latter steps of the secretory pathway in entamoeba histolytica. 200011070265
identification and characterizaton of a rab5 homologue in entamoeba histolytica. 200011070264
secretory pathway activation by interaction of entamoeba histolytica trophozoites with fibronectin. 200011070263
molecular genetics of the secretory pathway in entamoeba histolytica: an overview. 200011070262
identification of collagen-binding proteins from the cytoplasmic membrane of entamoeba histolytica. 200011070261
monoclonal antibody specific to the beta1 integrin-like molecule (140 kda) immunoprecipitates a protein complex of entamoeba histolytica. 200011070260
growth inhibition and actin aggregate formation of entamoeba histolytica by jasplakinolide. 200011070259
immunolocalization of clathrin during electron-dense granule secretion in entamoeba histolytica. 200011070258
the interplay between receptor capping and cytoskeleton remodeling in entamoeba histolytica. 200011070257
the diaphanous protein from entamoeba histolytica controls cell motility and cytokinesis. 200011070256
cytoskeleton activities during the interaction of entamoeba histolytica with epithelial cells. 200011070255
actin stress fibers in entamoeba histolytica induced by fibronectin. 200011070254
distribution of a potential p21-activated serine/threonine kinase (pak) in entamoeba histolytica. 200011070253
signal transduction pathways in entamoeba histolytica: pka activity and translocation during the interaction of trophozoites with fn. 200011070252
do entamoeba histolytica trophozoites signal via enteric microvilli? 200011070251
entamoeba histolytica induces cyclooxygenase-2 expression in macrophages during amebic liver abscess formation. 200011070250
effect of the prostaglandin analogue misoprostol on resistance to entamoeba histolytica infection in balb/c mice. 200011070249
metronidazole and mebendazole pretreatments suppress the antiamebic recognition of lamina propia lymphocyte supernatants from the small and large intestine in intraperitoneally immunized balb/c mice. 200011070248
effect of immunization with glutaraldehyde-fixed entamoeba histolytica trophozoites on the proportions of iga, igm, and igg b lymphocytes in the large and small intestine from balb/c mice. 200011070247
induction of protective immunity against experimental amebic abscess of the liver (eaal) using the monocyte locomotion inhibitory factor (mlif) produced by entamoeba histolytica: a preliminary report. 200011070246
parasite evasion mechanism altered by zinc in entamoeba histolytica. 200011070244
amebic lectin stimulation of lymphocytes of mexican mestizos recovered from amebic abscess of the liver induces decreased production of il5 and il6. 200011070240
does entamoeba dispar produce monocyte locomotion inhibitory factor (mlif) like entamoeba histolytica? 200011070239
effect of the monocyte locomotion inhibitory factor (mlif) produced by entamoeba histolytica on the expression of cell adhesion molecules (cams) in the skin of guinea pigs. 200011070238
the effect of the monocyte locomotion inhibitory factor (mlif) produced by entamoeba histolytica upon nitric oxide production by human leukocytes. 200011070237
compartmentalization of the intestinal antiamebic immune response in balb/c mice: effect of cholera toxin coadministration. 200011070234
modulated tgf-beta response in the hamster model in response to immunization with the recombinant l1b protein from entamoeba histolytica. 200011070233
isolation and structural studies of the monocyte locomotion inhibitory factor (mlif) produced by entamoeba histolytica. 200011070231
production of prostaglandin e(2) by entamoeba histolytica via a novel cyclooxygenase. 200011070230
lipophosphopeptidoglycan of entamoeba histolytica induces an antiinflammatory innate immune response and downregulation of toll-like receptor 2 (tlr-2) gene expression in human monocytes. 200011070229
the occurrence of a tripeptidyl peptidase i in trophozoites of entamoeba histolytica. 200011070227
homology modeling of entamoeba histolytica cysteine proteinases reveals the basis for cathepsin l-like structure with cathepsin b-like specificity. 200011070225
autokinase activities in entamoeba histolytica induced by extracellular matrix components. 200011070224
calcium binding protein of entamoeba histolytica. 200011070223
determination of entamoeba histolytica and entamoeba dispar infection by enzyme-linked immunosorbent assay (elisa) and its clinical correlation in pediatric patients. 200011070222
seroprevalence of anti-entamoeba histolytica antibodies by iha and elisa assays in blood donors from puebla, mexico. 200011070221
purification and biochemical characterization of three cysteine proteases of entamoeba histolytica with potential application in epidemiologic trials. 200011070220
incidence of entamoeba histolytica/entamoeba dispar in international travelers, extracommunitary immigrants, and adopted children. 200011070219
differentiation of entamoeba histolytica and entamoeba dispar using pcr-shela and comparison of antibody response. 200011070218
prevalence of entamoeba histolytica and entamoeba dispar in a highly endemic rural population. 200011070214
cytological and immunological methods to identify entamoeba histolytica and entamoeba dispar trophozoites in patients from guanajuato, mexico. 200011070213
a simple stool concentration method for the detection and preservation of the vegetative forms of entamoeba histolytica/entamoeba dispar. 200011070212
systemic antiamebic antibodies induced orally in mice: detection by an elisa test using preserved entamoeba histolytica-antigens without enzymatic inhibitors. 200011070211
diagnostic tests using idiotype expression in amebiasis. 200011070210
antibodies against entamoeba histolytica hemolysin hly4 in sera from patients with invasive amebiasis. 200011070209
characterization of plasma membrane-associated antigens of diagnostic and prophylactic importance in entamoeba histolytica. 200011070208
antiamebic activity of the metronidazole-zinc association. 200011070207
antiamebic activity of aqueous extracts from five medicinal plants from northeastern mexico. 200011070206
in vivo inhibition of reduced thiol compounds from entamoeba histolytica hk9 by phenothiazines and related tricyclic drugs. 200011070205
phagocytosis of quinfamide particles by entamoeba histolytica. 200011070204
effect of cyclosporine a on entamoeba histolytica. 200011070203
a new approach for chemotherapy against entamoeba histolytica. 200011070202
reincidence of amebic liver abscess: a case report. 200011070200
iron hydrogenases and the evolution of anaerobic eukaryotes.hydrogenases, oxygen-sensitive enzymes that can make hydrogen gas, are key to the function of hydrogen-producing organelles (hydrogenosomes), which occur in anaerobic protozoa scattered throughout the eukaryotic tree. hydrogenases also play a central role in the hydrogen and syntrophic hypotheses for eukaryogenesis. here, we show that sequences related to iron-only hydrogenases ([fe] hydrogenases) are more widely distributed among eukaryotes than reports of hydrogen production have suggested. ge ...200011070057
early lateral transfer of genes encoding malic enzyme, acetyl-coa synthetase and alcohol dehydrogenases from anaerobic prokaryotes to entamoeba histolytica.the fermentation enzymes, which enable the microaerophilic protist entamoeba histolytica to parasitize the colonic lumen and tissue abscesses, closely resemble homologues in anaerobic prokaryotes. here, genes encoding malic enzyme and acetyl-coa synthetase (nucleoside diphosphate forming) were cloned from e. histolytica, and their evolutionary origins, as well as those encoding two alcohol dehydrogenases (adhe and adh1), were inferred by means of phylogenetic reconstruction. the e. histolytica m ...200011069669
arbitrarily primed pcr fingerprinting of rna and dna in entamoeba histolytica.differences were detected in the gene expression of strains of e. histolytica using rna (rap-pcr) and dna fingerprinting (rapd). analysis of the electrophoretic profiles of the gels revealed some polymorphic markers that could be used in the individual characterization of the strains. the 260 bands generated by using five different primers for rap-pcr, as well as rapd, were employed in the construction of dendograms. the dendogram obtained based on the rapd products permitted the distinction of ...200011058934
intestinal amoebiasis: delayed-type hypersensitivity response in mice.delayed-type hypersensitive (dth) response was evaluated in c3h/hej mice intestinally infected with entamoeba histolytica. infected and non-infected control mice were challenged with amoebic antigen on day 5, 10, 15, 20, 25, 30, 40, 50, and 60 post-infection. maximum footpad swelling was observed after 24 hours of the challenge. the e. histolytica-infected mice exhibited a dth response on day 5, 15, 20, 25, 40 and 60 post-infection. however, on day 10, 30, and 50, such response was similar to th ...200011057067
characteristics of serine acetyltransferase from escherichia coli deleting different lengths of amino acid residues from the c-terminus.some properties of serine acetyltransferases (sats) from escherichia coli, deleting 10-25 amino acid residues from the c-terminus (satdeltac10-deltac25) were investigated. the specific activity depended only slightly on the length of the c-terminal region deleted. although the sensitivity of satdeltac10 to inhibition by l-cysteine was similar to that for the wild-type sat, it became less with further increases in the length of the amino acid residues deleted. satdeltac10 was inactivated on cooli ...200011055390
synthesis and antiamoebic activity of new cyclooctadiene ruthenium(ii) complexes with 2-acetylpyridine and benzimidazole derivatives.reaction of [ru(eta4-c8h12) (ch3cn)2 cl2] with 2-(2-pyridyl) benzimidazole or schiff bases derived from 2-acetylpyridine and s-methyldithiocarbazate, s-benzyldithiocarbazate and thiosemicarbazide leads to form new complexes of the type [ru(eta4-c8h12)(l)cl2] (where l=ligand). in vitro, most of the compounds exhibited potent activity and the ru derivatives 1a [ru(eta4-c8h12)(2-acpy-smdt)cl2], 2a [ru(eta4-c8h12)(2-acpy-sbdt)cl2] and 3a [ru(eta4-csh12)(2-acpy-tsc)cl2] were found more active than me ...200011055329
entamoeba histolytica: diminution of erythrophagocytosis, phospholipase a(2), and hemolytic activities is related to virulence impairment in long-term axenic cultures. 200011052870
outbreak of amebiasis in a family in the netherlands.human-to-human transmission of entamoeba histolytica is rare in industrialized countries. we describe an outbreak of amebiasis in a family in the netherlands, demonstrating that even with western standards of hygiene, persistent cyst passage may result in the transmission of e. histolytica to household contacts. if e. histolytica is isolated from a person living in an area of nonendemicity, it may be worthwhile to test all family members for cyst passage.200011049797
[amebiasis]. 200011043054
identification of two entamoeba histolytica sequence-specific ure4 enhancer-binding proteins with homology to the rna-binding motif rrm.to study transcriptional regulation in the lower branching eukaryote entamoeba histolytica, we have identified two sequence-specific dna-binding proteins that recognize the upstream regulatory element ure4, an enhancer that regulates expression of the gal/galnac lectin heavy subunit gene hgl5. a chromatographic purification of e. histolytica nuclear extracts by gel filtration, cation exchange, and sequence-specific dna affinity chromatography led to a 700-fold increase in ure4 binding activity a ...200111038357
entamoeba histolytica: deletion of the gpi anchor signal sequence on the gal/galnac lectin light subunit prevents its assembly into the lectin heterodimer.adherence and cytotoxicity of entamoeba histolytica require the function of a heterodimeric galactose and n-acetylgalactosamine (gal/galnac)-specific lectin. the lectin heavy subunit (hgl) contains a carbohydrate recognition domain and mediates inside-out cell signaling via its cytoplasmic tail. the function of the lectin light subunit (lgl) is unknown. the lectin has a unique mechanism of membrane association: hgl is transmembrane but lgl is glycosylphosphatidylinositol (gpi) anchored. the role ...200011038322
entamoeba histolytica: bacterial expression of a human monoclonal antibody which inhibits in vitro adherence of trophozoites. 200011038321
entamoeba histolytica: rapid detection of indian isolates by cysteine proteinase gene-specific polymerase chain reaction.amoebiasis, caused by entamoeba histolytica, is still one of the major problems for developing countries like india. early detection of the parasite is a must for its prevention and control. in this study, pcr analysis of the cysteine proteinase gene from clinical isolates of symptomatic intestinal and amoebic liver abscess (ala) cases has been compared with the stool microscopy, serology, and ultrasonography methods. the clinical isolates negative for e. histolytica by stool microscopy demonstr ...200011038312
[diagnosis of intestinal amebiasis using coproscopic and immunological methods in a population sample in greater metropolitan belém, pará, brazil].we compare diagnostic methods for entamoeba histolytica in fecal samples from the city of belém, pará, brazil. we analyze stool samples from children and adults (group i); stool and serum samples from adults (group ii); and stool samples from children (group iii). in groups i and iii, we used direct examination with lugol (dm), faust et al (fm), and elisa (detection of e. histolytica anti-giap coproantigen) and in group ii, dm, iron hematoxylin staining (ihs), fm, elisa, and the indirect immunof ...200011035523
immunodulation of rat serum and mucosal antibody responses to entamoeba histolytica trophozoites by beta-1,3-glucan and cholera toxin.systemic and mucosal and immune responses can be manipulated with immunomodulators. here we show the modulatory effects of cholera toxin (ct) and beta-1,3-glucan (glu) on the rat antiamebic serum and fecal antibody responses to one or four intraperitoneal (ip) or intragastric (ig) doses of glutaraldehyde-fixed entamoeba histolytica trophozoites (gft). one ip dose of gft maximized serum igm and igg antiamebic antibodies on days 4 and 9, respectively; ct coadministration increased igm antibodies, ...200011027459
molecular epidemiology of entamoeba spp.: evidence of a bottleneck (demographic sweep) and transcontinental spread of diploid parasites.entamoeba histolytica causes amebic colitis and liver abscess in developing countries such as mexico and india. entamoeba dispar is morphologically identical but is not associated with disease. here we determined the ploidy of e. histolytica and developed pcr-based methods for distinguishing field isolates of e. histolytica or e. dispar. fluorescence in situ hybridization showed that e. histolytica trophozoites are diploid for five "single-copy" probes tested. intergenic sequences between supero ...200011015408
identification of enteric pathogens in hiv-positive patients with diarrhoea in northern india.enteric pathogens associated with chronic diarrhoea in hiv-positive patients were studied. the study was conducted during january 1995-december 1998. stool specimens from all diarrhoea patients (n = 26) were examined microscopically for ova and parasites using wet preparations and stained smears. stool samples from diarrhoea patients were also cultured on appropriate media to isolate enteric bacterial pathogens. of the 59 patients, 26 (44%) had prolonged diarrhoea for more than 4 weeks. enteric ...200011014766
immunoblot analysis of iga antibodies to naegleria fowleri in human saliva and serum.the objective of this study was to evaluate the secretory iga (siga) antibody response to naegleria fowleri (nf) in individuals living in a parasite endemic area. saliva and serum samples were obtained from both healthy subjects and patients suffering from a respiratory illness (chronic bronchitis or rhinitis) and were analyzed by immunoblot assay. siga from the patients' samples recognized more intensely a greater number of nf proteins than did siga from the healthy control group. the proteins ...200011002989
direct amplification of entamoeba histolytica dna from amoebic liver abscess pus using polymerase chain reaction.an important and serious complication of intestinal infection with entamoeba histolytica is the involvement of the liver (hepatic amoebiasis). hepatic amoebiasis is usually diagnosed by the clinical picture (pain in the right upper quadrant and fever), ultrasound examination and positive serology. however, none of these tests are definitive and the picture overlaps with pyogenic liver abscess caused by bacteria. it is for this reason that the feasibility of using polymerase chain reaction (pcr) ...200011002979
the primordial high energy compound: atp or inorganic pyrophosphate?the pyrophosphate-dependent phosphofructokinase (pp(i)-pfk) of entamoeba histolytica displays a million fold preference for inorganic pyrophosphate (pp(i)) over atp (calculated as the ratio of k(cat)/k(m)). the introduction of a single mutation by site-directed mutagenesis changes its preference from pp(i) to atp. the single mutant has an 8-fold preference for atp whereas a related double mutant shows a preference exceeding 10,000-fold. the results suggest the presence of a latent nucleotide bin ...200011001940
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