Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| children's responses to different forms of expression of anger between adults. | anger is not a homogeneous stimulus, but can vary on a variety of dimensions and domains. this study examined children's responses to anger as a function of: (a) the mode of expression of anger (nonverbal, verbal, verbal-physical), and (b) whether or not anger between others was resolved. children were presented with videotaped segments of angry and friendly interactions and asked questions concerning their responses. all angry interactions, including non-verbal anger, were perceived as negative ... | 2008 | 2612248 |
| children's responses to different forms of expression of anger between adults. | anger is not a homogeneous stimulus, but can vary on a variety of dimensions and domains. this study examined children's responses to anger as a function of: (a) the mode of expression of anger (nonverbal, verbal, verbal-physical), and (b) whether or not anger between others was resolved. children were presented with videotaped segments of angry and friendly interactions and asked questions concerning their responses. all angry interactions, including non-verbal anger, were perceived as negative ... | 2008 | 2612248 |
| histotypic differentiation of neonatal mouse retina in organ culture. | retinae from neonatal mice were explanted in toto, with or without the retinal pigment epithelium (rpe) and adjoining mesenchymal cells, and maintained in organ culture for up to 3 weeks. the explants remained flat, rosette formation was minimal and histogenetic changes followed in the normal sequence. after 11, 14 and 21 days in vitro the three cellular layers--the outer nuclear layer including well differentiated rod and cone perikarya, the inner nuclear layer and the ganglion cell layer--with ... | 1989 | 2612197 |
| histotypic differentiation of neonatal mouse retina in organ culture. | retinae from neonatal mice were explanted in toto, with or without the retinal pigment epithelium (rpe) and adjoining mesenchymal cells, and maintained in organ culture for up to 3 weeks. the explants remained flat, rosette formation was minimal and histogenetic changes followed in the normal sequence. after 11, 14 and 21 days in vitro the three cellular layers--the outer nuclear layer including well differentiated rod and cone perikarya, the inner nuclear layer and the ganglion cell layer--with ... | 1989 | 2612197 |
| cockroaches (periplaneta americana) as carriers of agents of bacterial diarrhoea in accra, ghana. | bodies and intestinal contents of 208 cockroaches (periplaneta americana), collected from kitchens in accra and some surrounding villages were cultured for enteric bacterial pathogens. six of them harboured three different serogroups of salmonella, one had shigella dysenteriae, 64 had coliforms, 13 had proteus species, two had pseudomonas species and the rest (122) carried none of the bacterial species mentioned above. the presence of salmonella species shigella dysenteriae and coliforms in thes ... | 1989 | 2611875 |
| measles vaccine. | 2008 | 2602773 | |
| [risk factors for condylomata. a case control study]. | the present study involves 214 patients whose cervical smear showed signs of human papilloma virus infection (hpv), matched with 1042 controls. we demonstrate increasing odds ratio with the total number of sexual partners since first sexual intercourse (p less than 0.0001). moreover the cases have more often changed partners in the year preceding the diagnosis (odds ratio 2.6 p less than 0.0001). their male partners more often have occupations that expose them to "extra-conjugal adventures" (p = ... | 1989 | 2600372 |
| short-term structured treatment for avoidant personality disorder. | forty-two men and 34 women (mean age 27.5 years) who met dsm-iii criteria for avoidant personality disorder were randomly assigned to 1 of 3 treatment conditions or to a waiting-list control group. treatment ss who participated in a 10-week group treatment program displayed significantly greater improvement on a variety of self-report and behavioral measures than did untreated control subjects. the inclusion of skills-training procedures did not contribute to the effects of graduated exposure pr ... | 2008 | 2600246 |
| cadmium and lead accumulation by goldfish exposed to aqueous refuse incinerator fly ash leachate. | 2008 | 2597788 | |
| one year's experience using a rotating filter for therapeutic plasma exchange. | the authors previously demonstrated the feasibility of using a rotating filter system for therapeutic plasma exchange. they now report on the technical details of a 1 year clinical experience. seventeen patients underwent 188 treatments. hemoaccess was provided by antecubital veins (147 rx), femoral catheters (37 rx), or an a-v fistula (3 rx). blood flows ranged from 75 to 100 ml/min. net plasma removed per treatment was 3,231 +/- 53 ml (mean +/- se, n = 188). mean plasma removal rate per treatm ... | 2008 | 2597461 |
| causes of death and the histopathologic findings in fatal shigellosis. | thirty-seven children (median age, 2 years) with shigellosis in bangladesh were subjected to postmortem examination to determine causes of death and the spectrum of intestinal histopathology. infecting species were: shigella dysenteriae 1, 7 cases; s. dysenteriae 2, 2 cases; shigella flexneri, 23 cases; shigella boydii, 4 cases; and mixed infection with shigella boydii and shigella sonnei, 1 case. complicating conditions detected before death included malnutrition in 25 cases, pneumonia in 11 ca ... | 1989 | 2594452 |
| expression and desensitisation of a2 purinoceptors on cultured bovine aortic endothelial cells. | purine nucleotides and their metabolites are important mediators of vascular tone. adenosine promotes relaxation of vascular smooth muscle, acting on the a2 subclass of purinoceptors. there is some evidence that these receptors are also present on vascular endothelial cells. we have therefore examined cultured aortic endothelial cells for adenosine (a2) sensitivity. bovine aortic endothelial cells (ag4762) were obtained from the institute of aging cell repository (usa), and cultured in monolayer ... | 1989 | 2590913 |
| melanin concentrating hormone (mch): structure-function aspects of its melanocyte stimulating hormone-like (msh-like) activity. | melanin concentrating hormone (mch) is a heptadecapeptide, asp-thr-met-arg-cys-met-val-gly-arg-val-tyr-arg-pro-cys-trp-glu-val, synthesized in the brain and secreted from the pars nervosa of teleost fish. this hormone stimulates melanosome (melanin granule) aggregation within integumental melanocytes of fishes but, in contrast, stimulates melanosome dispersion within tetrapod (frog and lizard) melanocytes. we determined the message sequence of the primary structure of mch which is responsible fo ... | 2008 | 2587419 |
| altered queuine modification of transfer rna involved in the in vitro transformation of chinese hamster embryo cells. | altered queuine modification of trna has been correlated to neoplastic transformation, but no direct cause and effect relationship has been defined. in the present study, a potential role for this alteration has been assigned. the trna in normal chinese hamster embryo cells is significantly more queuine modified than the trna in their transformed chinese hamster embryo counterparts, even though the specific activity of the queuine modification enzyme is much lower in chinese hamster embryo cells ... | 2008 | 2582452 |
| regulation of cell proliferation and differentiation by interferons. | 2008 | 2581554 | |
| the company they keep. fifth annual meeting on oncogenes sponsored by the foundation for advanced cancer studies, inc., frederick, md, usa, june 27-july 1, 1989. | 2007 | 2577367 | |
| the company they keep. fifth annual meeting on oncogenes sponsored by the foundation for advanced cancer studies, inc., frederick, md, usa, june 27-july 1, 1989. | 2007 | 2577367 | |
| [differential indications, surgical technics and follow-up treatment concepts of elbow joint surgery in polyarthritis]. | 2008 | 2576707 | |
| [ki-1 large cell lymphoma with secondary xanthomatous infiltration of the lesions by chemotherapy]. | 2008 | 2575882 | |
| the effect of an aversive event on the return of fear. | an experiment on the return-of-fear (rof) was carried out on 40 snake- or spider-phobic subjects in order to determine whether an arousing event that occurs shortly before retest influences the magnitude of the rof. additionally, we attempted to produce a reinstatement of fear by introducing an unrelated aversive event (shock) after the fear had been reduced. the arousal manipulation successfully increased subjective arousal but not heartrate responsiveness. the increases in subjective arousal w ... | 2008 | 2573336 |
| shigella dysenteriae type 1 infections in us travellers to mexico, 1988. | in 1988, the number of shigella dysenteriae type 1 (sd1) infections reported in the usa increased five-fold over the annual mean from the previous decade. 44 (94%) of 47 interviewed patients reported recent travel to mexico; 33 (75%) of these had been tourists to the yucatan peninsula. 27 patients who had travelled to mexico were admitted to hospital, of whom 2 had a haemolytic uraemic syndrome; none died. the antimicrobial resistance pattern and plasmid profile of the yucatan strain were simila ... | 1989 | 2570242 |
| intraspinal opioids: implications for monitoring. monitoring in the intensive care unit is essential. | patients receiving intraspinal opiates should be monitored in the intensive care unit for at least 24 hours to prevent potentially lethal outcomes. these include respiratory depression caused by sequestration of the morphine in the cerebrospinal fluid and migration of epidural catheters in the subarachnoid or intravascular space. at this time, most hospitals are not equipped or staffed adequately to guarantee the safety of these patients outside the intensive care unit. | 2008 | 2570133 |
| endocytosis from coated pits of shiga toxin: a glycolipid-binding protein from shigella dysenteriae 1. | evidence is presented that endocytosis is involved in the transport to the cytosol of the cytotoxin from shigella dysenteriae 1, shiga toxin, which acts by removal of a single adenine residue in 28-s ribosomal rna. inhibition of endocytosis by atp depletion of the cells prevented toxin uptake. exposure of hela s3 and vero cells to toxin at low extracellular ph, where translocation to the cytosol, but not endocytosis is inhibited, allowed the toxin to accumulate in a compartment where it was prot ... | 1989 | 2564398 |
| vecuronium infusion requirements in pediatric patients during fentanyl-n2o-o2 anesthesia. | eighty-one pediatric patients, ranging from neonates to adolescents, were studied during fentanyl-n2o-o2 anesthesia to determine for each of them the vecuronium infusion required to maintain 90-95% neuromuscular block (nmb). electromyographic monitoring with train-of-four stimuli was used. the steady infusion rate was 62 +/- 15 (sd) micrograms.kg-1.hr-1 in neonates and infants. this rate was 40% of that required by children 3 to 10 years old (154 +/- 49 micrograms.kg-1.hr-1; p less than 0.05). i ... | 2007 | 2562909 |
| [acute maternal anterior poliomyelitis in a non-endemic zone]. | the authors report the case of a 26 year old woman with acute anterior poliomyelitis contracted during the vaccination of her baby. despite having been herself vaccinated in infancy she was not protected against the poliovirus. the clinical interest of this uncommon case is a severe paralytic state with definitive paraplegia. the authors suggest serologic testing of patients born before 1967 especially if they are at risk of encountering the virus. | 1989 | 2561040 |
| characterization of the rela1 mutation and a comparison of rela1 with new rela null alleles in escherichia coli. | the most widely studied "relaxed" mutant of the rela locus, the rela1 allele, is shown here to consist of an is2 insertion between the 85th and 86th codons of the otherwise wild-type rela structural gene, which normally encodes a 743-amino acid (84 kda) protein. the rela protein is a ribosome-dependent atp:gtp (gdp) pyrophosphoryltransferase that is activated during the stringent response to amino acid starvation and thereby occasions the accumulation of guanosine 3',5'-bispyrophosphate (ppgpp). ... | 1989 | 2556396 |
| phosphate regulon in members of the family enterobacteriaceae: comparison of the phob-phor operons of escherichia coli, shigella dysenteriae, and klebsiella pneumoniae. | the structure and function of the phob and phor genes of shigella dysenteriae strains and klebsiella pneumoniae, which are involved in regulation of the phosphate regulon, were analyzed. complementation tests among the genes of escherichia coli, s. dysenteriae strains, and k. pneumoniae for production of alkaline phosphatase indicate that s. dysenteriae serotype 2 and serotype 3 strains and k. pneumoniae are phoa+ phob+ phor+ but s. dysenteriae sh and serotype 1 strains are phoa phob+ phor. nucl ... | 1989 | 2556368 |
| influence of diethyldithiocarbamate on nickel-induced hepatic metallothionein in rats. | the influence of sodium diethyldithiocarbamate (ddc) on 63nickel chloride-induced metallothionein (mt) and alterations in essential metal levels in the liver of rats was investigated. the induction of mt, zn and cu levels of the hepatic cytosolic "heat stable fraction" (hsf) by ddc increased with time up to 24 hr. although mt, zn and cu were significantly greater at 17 hr than those at 6.5 hr after 63ni administration, the ni level decreased. the treatment with ddc at 6 hr, but not at 10 min, pr ... | 1989 | 2548306 |
| [a germ cell tumor of an ectopic testis in polyorchidism]. | 2008 | 2547104 | |
| dual function of a nuclear factor i binding site in mmtv transcription regulation. | using linker-scanning mutagenesis we had previously identified four elements within the mmtv ltr which are necessary for transcriptional stimulation by glucocorticoid hormones. two of them overlapped with regions to which the glucocorticoid receptor binds in vitro. the third element contained a nf-i binding site, and the fourth the tata box. here we show that mutations that abolish in vitro binding of nf-i had a negative effect also on the basal activity of the mmtv promoter of ltr-containing pl ... | 1989 | 2542892 |
| multiple cooperative interactions constrain bpv-1 e2 dependent activation of transcription. | transcription directed by the bpv-1 long control region (lcr) is conditional upon activation by the virally encoded e2 protein. within the 1.0 kb lcr there are five separate regions, a to e, that contain e2 responsive enhancers. the smallest functional region, a, is only 38 bp and contains two copies of the consensus sequence acc(n)6ggt that is known to function as an e2 binding site in vitro. we show that a pair of these constitutes a minimal functional e2 responsive enhancer element but that t ... | 1989 | 2542891 |
| effects of the nmda receptor antagonist mk-801 against amygdaloid-kindled seizures in the rat. | the effects of acute and subchronic administration of the noncompetitive nmda receptor antagonist mk-801 on the duration and severity of amygdaloid-kindled seizures in rats were determined to evaluate its anticonvulsant activity. both the highest acute (1000 micrograms/kg) and subchronic regimens (2 x 300 micrograms/kg, 7 days) produced significant reductions in the seizure stage and afterdischarge duration. although ataxia may have affected the seizure stage, mk-801 attenuated the primary after ... | 1989 | 2536332 |
| [the dependence of hemoglobin and iron reserve indices on habitat conditions (sampling research in osh province, the kirghiz ssr)]. | sampling investigation of female population (896 subjects) was conducted in five regions with opposite environmental conditions. a relatively low incidence of iron deficiency anemia and high incidence of exhausted iron reserves in the women living in the high mountains indicates the participation of the adaptive-compensatory mechanisms in the development of iron-deficiency. a high rate of iron deficiency incidence in the regions of intensive tobacco growing and association of iron-deficiency ane ... | 1989 | 2533125 |
| identification of shiga-like toxin in escherichia coli strains, etiological agents of diarrheal disease. | shiga-like toxin presence, in 20 e. coli strains, etiological agents of diarrheal diseases, is studied by preparing extracts at +4 degrees c, in the presence of chloroform and by i.v. inoculation in mice. in 4 out of 20 strains, shiga-like toxin in high titres was identified. most of the strains presented an inconstant and variable production of shiga-like toxin in comparison with shigella dysenteriae type 1 (shiga) reference strain. the authors also confirm the existence of shiga-like toxin und ... | 1989 | 2520669 |
| [cardiac tamponade as the first manifestation of cancer]. | we report 3 patients having cardiac tamponade as the initial presentation of cancer. at the onset of symptoms all three patients were incorrectly diagnosed. pulsus paradoxus and cardiomegaly on the chest x-ray were the main clinical features. two dimensional echocardiography was vital revealing massive pericardial effusion with diastolic right atrial and ventricular collapse. all patients had bloody pericardial fluid removed and the histopathology of the metastatic pericardial tissue showed lung ... | 2008 | 2519419 |
| [evaluation of results of school health examination in the murcia region. 1986/87]. | in the present study, we have carried out an evaluation of school examinations in 47,943 children in the past course. we have compared our results with those from similar previous studies in other parts of spain. our findings were comparable in visual acuity and scoliosis, and not so much about cryptorchidism and phimosis. we think that examinations are necessary, but it is needed to increase the quality and the effectiveness, to get closer to the knowledge of usefulness and to the correct evalu ... | 2008 | 2518932 |
| [in vitro susceptibility of campylobacter jejuni and campylobacter coli to quinolone antibiotics]. | in this study, in vitro susceptibilities of 24 campylobacter strains isolated from fecal samples were tested for quinolone antibiotics. 13 of the strains were identified as c. jejuni and 11 of them were c. coli. antibiotics which were included in this study were ciprofloxacin, ofloxacin, pefloxacin and fleroxacin. antibiotic susceptibility tests were performed on mueller-hinton agar (supplemented with 5% sheep blood) using the agar dilution method. results showed that quinolone antibiotics are v ... | 1989 | 2518646 |
| [increased efficiency in the disinfection of sewage by lactic acid bacteria]. | the antimicrobic properties have been studied in 30 strains of lactobacilli. as a result a strain, the strongest antagonist relative to choleric vibrios and other enteropathogenic microorganisms, is selected. lactobacilli are found to retain their viability and biological activity in the activated sludge during the whole period of observation (6 months). biological disinfection of sewage is shown possible to be intensified using the activated sludge inoculated by the culture of the selected lact ... | 1989 | 2514340 |
| tongue flaps in repair of large palatal defects. | anteriorly based dorsal tongue flaps were used in 19 cleft patients for closing large palatal defects. the procedure was successful in 17 patients. one patient had partial marginal necrosis after division of the pedicle, while another had complete necrosis of the distal part of the flap. our results support the view that for success of the procedure, adequate length, width and thickness of the flap are important, while the height of the palate also plays a role. | 2008 | 2509583 |
| hemagglutinating properties of shigella dysenteriae type 1 and other shigella species. | strains of shigella dysenteriae type 1 cultured in casamino acids-yeast extract broth medium in the presence of 1 mm calcium chloride at 37 degrees c for 22 h induced hemagglutination of erythrocytes that was inhibited by n-acetylneuraminic acid, n-acetylneuramin-lactose, and alpha 1-glycoprotein. the hemagglutination was heat labile, and the absence of cell-surface appendages suggested a nonfimbrial adhesin(s). under the same conditions, strains of shigella flexneri (types 1a, 1b, 2a, and 2b) s ... | 1989 | 2503449 |
| four new provisional serovars of shigella. | four bacterial strains are described that possess the biochemical characteristics of shigella species but do not belong to any of the established shigella serovars or to any previously described provisional serovar. one strain fermented mannitol, and it is proposed that this be the type strain for a new provisional serovar of shigella boydii. the remaining strains did not ferment mannitol and belonged to three different serovars. these strains are proposed as type strains for three new provision ... | 1989 | 2501349 |
| nonrandom rearrangement of chromosome 14 at band q32.33 in human lymphoid malignancies with mature b-cell phenotype. | chromosomes were studied in 61 patients with differentiated b-cell malignancies including 21 with non-hodgkin's lymphoma (nhl), three with hairy cell leukemia (hcl), eight with waldenström's macroglobulinemia (wm), and 29 with plasma cell disorder. chromosomally abnormal clones were identified in 35 of 61 patients studied: all with nhl, all with hcl, three of eight with wm, and eight of 29 with plasma cell disorder. the most recurrent chromosomal abnormality, observed in 26 of the 35 patients wh ... | 1989 | 2492904 |
| [imported myiasis: analysis of 5 cases]. | five cases of furuncular myiasis imported by spanish travelers from tropical countries are reported. after reviewing the cycle of the infecting organism and the characteristics of the human disease, a table with the clinical and epidemiological data of our patients is presented. the relevance of an adequate clinical and geographical anamnesis in these patients is discussed. finally, the effectiveness of a simple, nonaggressive treatment is reported, which permits the patient to verify by himself ... | 1989 | 2490857 |
| morbidity pattern in aged persons attending rural health centre in haryana. | 1997 | 2486987 | |
| the complexion and complexity of the nursing profession. | 1995 | 2486684 | |
| studies on shiga-like toxin produced by enterohemorrhagic escherichia coli: purification and characterization of the toxin and development of methods for identifying the toxin. | a simple purification method using deae cellulose column chromatography and immunoaffinity column chromatography was developed for purifying shiga-like toxin produced by escherichia coli o157:h7. about 0.75 mg of purified toxin was obtained from 5 liters of culture (62% recovery). the purified toxin was demonstrated to be immunologically, biologically and structurally indistinguishable from shiga toxin. a sandwich enzyme-linked immunosorbent assay (elisa) was developed for detection of shiga-lik ... | 1989 | 2485395 |
| effect of detoxification processes on the interferon-inducing activity of bacterial endotoxins. | the interferon (ifn)-inducing activity of detoxified lipopolysaccharide (lps) was tested in rabbits treated with lps preparation derived from escherichia coli, salmonella typhi, salmonella enteritidis and shigella dysenteriae serovar 1. of the detoxification procedures used, alkaline hydrolysis, hydroxylaminolysis, formalization, treatment with sodium deoxycholate and the radiodetoxification (fast or slow) methods had no appreciable effects on the ifn-inducing potential of lps. in contrast, acet ... | 1989 | 2484274 |
| production and characterisation of monoclonal antibodies to verotoxins 1 and 2 from escherichia coli of serotype o 157:h7. | fourteen hybridoma cell lines were isolated that produced monoclonal antibodies (mabs) to purified verotoxins 1 and 2 (vt1, vt2) of escherichia coli of serotype o157:h7. of these mabs, eight were obtained by immunisation of balb/c mice with purified vt1, and six were obtained from balb/c mice immunised with purified vt2. with the exception of mab 1c5, with a heavy chain of igg2b class, antibodies produced from mice immunised with heat-treated toxin were of igm class. mabs produced from mice immu ... | 1989 | 2479749 |
| genetic and biochemical analysis of shigella dysenteriae 1 o antigen polysaccharide biosynthesis in escherichia coli k-12: structure and functions of the rfb gene cluster. | the genetic organization and functions of the shigella dysenteriae 1 rfb gene cluster, which specifies the somatic o antigen in this organism, have been studied in escherichia coli k-12 by insertion and deletion mutagenesis of pss9, a pbr322 hybrid containing the shigella rfb genes. on the basis of the sensitivity/resistance to rough-specific bacteriophage t3 of e. coli k-12 derivatives containing mutant pss9 plasmids, of the banding patterns and immunoreactivity of lps isolated from such deriva ... | 1986 | 2469933 |
| genetic and biochemical analysis of shigella dysenteriae 1 o antigen polysaccharide biosynthesis in escherichia coli k-12: 9 kb plasmid of s. dysenteriae 1 determines addition of a galactose residue to the lipopolysaccharide core. | production of the somatic antigen, o-specific polysaccharide of shigella dysenteriae 1 is determined by the chromosomal rfb gene cluster and the rfp gene located on the 9 kb plasmid phw400 carried by this organism. when transferred to escherichia coli k-12, which produces lipopolysaccharide consisting only of core oligosaccharide linked to lipid a, rfp gene-containing plasmids caused modification of the core oligosaccharide leading to the appearance of core molecules with new electrophoretic mob ... | 1986 | 2469932 |
| cloning of the rfb gene region of shigella dysenteriae 1 and construction of an rfb-rfp gene cassette for the development of lipopolysaccharide-based live anti-dysentery vaccines. | recent studies have shown that determinants for the production of o antigen lipopolysaccharide in shigella dysenteriae 1 are distributed over two distinct genetic elements, the chromosome and a 9 kb plasmid designated phw400. in this communication, we describe the cloning of all determinants necessary for s. dysenteriae 1 o antigen production in e. coli k-12 and their combination in a single plasmid. an rp4::minimu r-prime plasmid, r-prime 40, containing the his-rfb (histidine biosynthesis-lipop ... | 1986 | 2469931 |
| enteropathogens isolated from children with gastro-enteritis at ga-rankuwa hospital, south africa. | a prospective 3-year study for enteric pathogens associated with diarrhoea in children was undertaken at ga-rankuwa hospital, south africa. rotavirus was the most commonly isolated pathogen (24%), showing a marked seasonal distribution, with an autumn peak in activity. enterotoxigenic and enteropathogenic escherichia coli were frequently identified, and salmonella, shigella and campylobacter were also isolated. cryptosporidium and giardia lamblia were the most frequently seen parasites. rotaviru ... | 1988 | 2467616 |
| purification and characterization of a phage-encoded cytotoxin from an escherichia coli o111 strain associated with hemolytic-uremic syndrome. | cytotoxin production by escherichia coli o111:h-strain hus-2 (hamburg) is associated with a temperate toxin-converting bacteriophage (tcp-111). e. coli laboratory strain c600 transduced and subsequently lysed by the phage produced and liberated large amounts of cytotoxin (ct111) which was purified by sequential chromatography. when compared with published procedures for toxin release from viable cells, lysis of the c600 culture by the phage was most effective. by sds-page ct111 as shiga toxin fr ... | 1988 | 2464885 |
| somatic antigens of shigella: structure of the o-specific polysaccharide chain of the shigella dysenteriae type 7 lipopolysaccharide. | 4-(n-acetylglycyl)amino-4,6-dideoxy-d-glucose has been identified as a component of the shigella dysenteriae type 7 o-specific polysaccharide, in addition to the previously reported 2-acetamido-2-deoxy-d-glucose and 2-acetamido-2-deoxy-d-galacturonic acid. on the basis of selective cleavage with anhydrous hydrogen fluoride and analysis by 1h- and 13c-n.m.r. spectroscopy and f.a.b.-mass spectrometry, it was concluded that the tetrasaccharide repeating-unit of the polysaccharide has the following ... | 1988 | 2463086 |
| ifn-treated neuroblastoma cell lines remain resistant to t cell-mediated allo-killing, and susceptible to non-mhc-restricted cytotoxicity. | neuroblastoma cell lines can have very low mhc ag expression. the cell lines are insensitive to allo-killing by primed ctl, but are sensitive to non-mhc-restricted cytotoxicity. ifn-gamma increased class i expression, but the cells remained insensitive to ctl. susceptibility to nonrestricted effectors was preserved. class i+ glioma cell lines behaved similarly. the ctl resistance was localized to the recognition phase. neuroblastoma lines did not form conjugates with primed t cells, but were lys ... | 2008 | 2459235 |
| epitope mapping of hla-b27 and hla-b7 antigens by using intradomain recombinants. | to study the hla-b7 and hla-b27 antigenic determinants, hybrid genes between these two alleles were constructed by in vivo recombination in escherichia coli. after transfection of these genes into p815 (high transfection efficiency recipient) murine cells, the bindings of bw6, hla-b7, and hla-b27 allele-specific mab were studied, as well as that of human anti-hla-b7 and anti-hla-b27 monospecific alloantisera. most of the hla-b7 antigenic determinants were assigned to the first external domain of ... | 1988 | 2459214 |
| the stability of o-antigen plasmid is determined by a chromosomal region of shigella dysenteriae 1. | it is well established that plasmids are involved in the expression of lipopolysaccharide in certain species of shigella. in shigella sonnei, both the biosynthesis of oligosaccharide side chains (o antigen), and cell invasiveness are controlled exclusively by a 120 megadalton (mda) plasmid. in shigella dysenteriae 1, a 10 kilobase (kb) plasmid is required for o-antigen production. shigella dysenteriae 1 strains devoid of this plasmid lose the ability to synthesize o antigen. interestingly, this ... | 1988 | 2454151 |
| structural homology of human complement component c8 gamma and plasma protein hc: identity of the cysteine bond pattern. | anti-c8 alpha-gamma specific antibodies were used to isolate cdna clones from a human liver expression library. antibodies affinity-purified on the expressed hybrid protein of one clone bound exclusively to the gamma-chain of reduced c8 alpha-gamma. this clone, as well as a second full length cdna clone obtained by hybridization screening, were sequenced and the complete primary structure for c8 gamma was established. cyanogen bromide cleavage of c8 alpha-gamma released a 12 kda carboxy-terminal ... | 1987 | 2447883 |
| effects of substance p, neurokinin a and calcitonin gene-related peptide in human skin and their involvement in sensory nerve-mediated responses. | the effects evoked by intradermal injections of substance p (sp), neurokinin a (nka) or calcitonin gene-related peptide (cgrp) were studied in 51 non-atopic subjects. sp and nka produced flare and weal, and cgrp produced an indurated erythema. the reactions to sp were strong, the flare being maximal 3-5 min after injection and the weal after 10-15 min. nka evoked a much weaker flare and a slightly weaker weal than did sp. cgrp produced a prominent long-lasting, indurated erythema with pseudopod ... | 1987 | 2446892 |
| comparative effects of adriamycin and dna-non-binding analogues on dna, rna, and protein synthesis in vitro. | drug-dna binding is claimed to be the basis by which the antitumor antibiotic adriamycin (doxorubicin) inhibits dna and rna synthesis in vitro. however, in preliminary studies the dna-non-binding adriamycin analogue n-trifluoroacetyladriamycin-14-valerate (ad 32) showed somewhat greater inhibition of dna and rna synthesis than adriamycin under identical conditions. the kinetics of macromolecule synthesis inhibition induced by adriamycin and ad 32, and the two principal dna-non-binding metabolite ... | 1987 | 2446792 |
| the effects of the protein synthesis inhibitor anisomycin on the febrile responses to intracerebroventricular injections of bacterial pyrogen, arachidonic acid and prostaglandin e2. | 1. anisomycin (15 mg/kg) was administered s.c. to cats at ambient temperatures of 5 degrees c, 20 degrees c and 38 degrees c. it produced biphasic effects on body temperature at 5 degrees c and 20 degrees c, an initial fall in temperature followed by a rise in body temperature, and a rise in body temperature of long latency at 38 degrees c. 2. anisomycin (15 mg/kg) attenuated the hyperthermic responses to centrally injected pge2 (1 microgram) at all ambient temperatures studied and also complete ... | 1987 | 2446153 |
| [structure of the o-specific polysaccharide chain of shigella dysenteriae type 7 lipopolysaccharide]. | the structure of the o-specific polysaccharide chain of the shigella dysenteriae type 7 lipopolysaccharide has been established mainly by 13c nmr analysis of the intact and modified (acetylated and de-o-acetylated) polymers, as well as of products of its solvolysis with anhydrous hydrogen fluoride. the polysaccharide contains two unusual sugar derivatives. n-acetyl-d-galactosaminuronamide and 4-(n-acetylglycyl)amido-4,6-dideoxy-d-glucose (galnacan and qui4n----glyac, respectively) and is built u ... | 1987 | 2445353 |
| a mucin containing the x, y, and h type 2 carbohydrate determinants is shed by carcinoma cells. | monoclonal antibody br 15-6a directed to the y carbohydrate determinant (fuc alpha 1----2gal beta 1----4glcnac(3----1fuc) beta 1----3gal beta 1----4glc beta 1----1cer) reacted with the cell surface and conditioned media of colorectal and breast carcinoma cell lines. double determinant immunoassays using br 15-6a as detector antibody showed that the y determinant is part of a high molecular weight mucin that coexpressed other carbohydrate antigens based on a type 2 chain (x, h type 2). type 1 cha ... | 2008 | 2442094 |
| [photocoagulation with krypton laser of subretinal vessels in macular degeneration related to age. long-term results (apropos of 100 cases)]. | 2008 | 2440603 | |
| monoclonal antibodies specific for shigella flexneri lipopolysaccharides: clones binding to type iv, v, and vi antigens, group 3,4 antigen, and an epitope common to all shigella flexneri and shigella dysenteriae type 1 stains. | monoclonal antibodies reactive with shigella flexneri o antigens were generated in both mouse and rat systems. antibody-producing hybridomas were screened in an enzyme-linked immunosorbent assay using chemically defined lipopolysaccharides as antigens, and the epitope specificities were determined with a panel of lipopolysaccharides and synthetic o-antigen-specific glycoconjugates as antigens. to verify the specificity seen in the enzyme-linked immunosorbent assay, the antibodies were used in ag ... | 1987 | 2437036 |
| component of whole cell ca current due to electrogenic na-ca-exchange in cardiac myocytes. | a carrier mechanism that mediates an exchange of na+ for ca2+ across the cell membrane is believed to be an important regulator of intracellular ca2+ and therefore the contractile strength of the heart. considerable evidence indicates that there is an unequal transfer of electrical charge during this exchange which implies that the carrier may contribute substantially to the net flow of ionic current across the sarcolemmal membrane. to detect such a component of current, nondialyzed single frog ... | 2006 | 2435177 |
| sporidesmin and gliotoxin induce cell detachment and perturb microfilament structure in cultured liver cells. | changes in cell morphology and cell adhesion occurred when cultured cells from the rat liver cell strain c3 were exposed to the fungal toxins, sporidesmin or gliotoxin. both toxins caused loss of attachment of the cells to the plastic of tissue culture plates and this effect was preceded by loss of actin cables. other changes included cytoplasmic vacuolation and blocked entry into s-phase of the cell cycle. under these conditions [3h]thymidine incorporation into the cells was also diminished but ... | 1986 | 2432076 |
| immunofluorescence detection of cryptosporidium oocysts in fecal smears. | an indirect fluorescent antibody (ifa) procedure was developed for the detection of cryptosporidium sp. oocysts in human, nonhuman primate, and bovine fecal smears. the procedure, which takes about 90 min to perform, involves the use of a rabbit antiserum against cryptosporidium oocysts isolated from dairy cattle. cross-specificity testing of the ifa method revealed no reactivity with yeasts, various amoebae, giardia lamblia, chilomastix sp., or blastocystis sp. and only very weak cross-reactivi ... | 1986 | 2429982 |
| prothrombin requires two sequential metal-dependent conformational transitions to bind phospholipid. conformation-specific antibodies directed against the phospholipid-binding site on prothrombin. | prothrombin is a gamma-carboxyglutamic acid-containing protein that binds to phospholipid vesicles in the presence of calcium ions after undergoing a metal ion-induced conformational transition. to integrate recent data into a scheme that is compatible with our knowledge of prothrombin-metal interaction, we have proposed a new model of prothrombin structure. in this model prothrombin undergoes two metal-dependent conformational transitions: pt----pt'----pt*. the first transition is not cation-sp ... | 2008 | 2429962 |
| growth factors, angiogenesis and metastasis. | 1986 | 2424027 | |
| [vital staining of the conjunctiva for prevention of epithelial implantation cysts]. | selective vital staining with 1% toluidine blue makes short-term intraoperative differentiation of the conjunctiva from tenon's capsule possible. it is used with advantage in operations where the anatomical situation is unclear to avoid displacement of conjunctival epithelium into deeper tissues and subsequent growth of cysts. examples of its application are reoperations with excessive scars after squint or detachment surgery, wound dehiscence after enucleations, lacerated wounds after accidents ... | 1985 | 2415747 |
| inactivation by polymyxin b of the endotoxin-mediated interferon production in the rabbit. | polymyxin b (pb) completely or at least significantly inhibited the capacity of shigella dysenteriae 1 cells and the lipopolysaccharide (lps) and lipid a (la) subunits of several bacterial endotoxins to induce interferon (ifn) in rabbits. animals injected with lps inactivated by pb to the point of not inducing detectable ifn levels did not develop hyporesponsiveness to secondary ifn induction by a homologous inducer. it was concluded that pb inhibits the ifn-inducing capacity of endotoxin and it ... | 1985 | 2413651 |
| 'o'-antigens are essential virulence factors of shigella sonnei and shigella dysenteriae 1. | a novel transposon mutagenesis system has been developed for shigella. we have used it to isolate specific mutants of shigella sonnei and shigella dysenteriae 1 that fail to produce lipopolysaccharide 'o' side chains. the virulence of the mutants was evaluated in the sereny test and in a hela cell invasion assay. all ho'-minus mutants failed to provoke a positive sereny reaction but retained the ability to invade hela cells. this demonstrates that 'o' side chains are virulence factors of s. sonn ... | 1985 | 2413650 |
| axonally transported shigella cytotoxin is neuronotoxic. | shigella dysenteriae strains produce an exotoxin (sdt) which inhibits protein synthesis in susceptible cells and is neurotoxic in some species. intraneural microinjection of highly purified sdt into the cervical vagus nerves of rats, mice, guinea pigs and rabbits produced cytopathic changes within 24 hours in vagal sensory but not motor neurons. these changes consisted of an initial loss of nissl substance followed by progressive cell degeneration and resulted in permanent neuronal loss. indirec ... | 1985 | 2411880 |
| the colon in shigellosis: serial colonoscopic appearances in shigella dysenteriae i. | twenty-five patients (16 males and 9 females; mean age 24.7 +/- 8.4 years) with acute colitis caused by shigella dysenteriae i were studied, a total of 115 colonoscopic examinations being performed. twenty-two patients had weekly colonoscopic examinations until the last procedure revealed normal colonic mucosa. six abnormal colonoscopic appearances were documented, namely mucosal edema, ulcers, friability, punctate spots, erythematous areas and luminal exudate. mucosal edema was the dominant fin ... | 1990 | 2407526 |
| functional analysis of the shiga toxin and shiga-like toxin type ii variant binding subunits by using site-directed mutagenesis. | the b subunit of shiga toxin and the shiga-like toxins (slts) mediates receptor binding, cytotoxic specificity, and extracellular localization of the holotoxin. while the functional receptor for shiga toxin, slt type i (slt-i), and slt-ii is the glycolipid designated gb3, slt-ii variant (slt-iiv) may use a different glycolipid receptor. to identify the domains responsible for receptor binding, localization in escherichia coli, and recognition by neutralizing monoclonal antibodies, oligonucleotid ... | 1990 | 2404947 |
| role of ceramide as a lipid mediator of 1 alpha,25-dihydroxyvitamin d3-induced hl-60 cell differentiation. | the treatment of hl-60 myelocytic leukemia cells with 1 alpha,25-dihydroxyvitamin d3 (1,25-(oh)2d3) resulted in the activation of a neutral sphingomyelinase and in sphingomyelin turnover (okazaki, t., bell, r., and hannun, y. (1989) j. biol. chem. 264, 19076-19080). in this paper, the effects of 1,25-(oh)2d3 on the product of sphingomyelin hydrolysis, ceramide, and the possible function of ceramide as a lipid mediator of the effects of 1,25-(oh)2d3 on hl-60 cell differentiation were investigated ... | 2007 | 2394750 |
| a method for detecting shiga toxin and shiga-like toxin-i in pure and mixed culture. | shiga toxin and shiga-like toxins (slts, syn. verotoxins) are currently detected by tissue culture assays that are expensive, time-consuming and require specialised facilities and experienced personnel. we have developed a rapid method to detect shiga toxin and slt-i (verotoxin 1) based on their binding to globotriosyl ceramide (gb3). bound toxin was then detected by an enzyme-linked immunosorbent assay (elisa) with monoclonal antibodies. the direct detection of cytotoxins from pure culture plat ... | 1990 | 2391709 |
| tissue integration of a12o3-ceramic dental implants (frialit)--a case report. | the tissue intraosseous integration of a12o3-ceramic dental implants (frialit) is studied in a patient case, where an implant-fracture made it necessary to remove the implant from a patient's jaw. the surgical removing of the apical part of the implant with the use of a trepan bur made it possible to examine and visualize histologically and microradiographically the tissue adjacent to the implant. the results of both the histological and microradiographical studies showed that a good healing and ... | 2007 | 2374995 |
| role of bacterial and host factors in the pathogenesis of shigella septicemia. | to determine the role of bacterial and host factors in the pathogenesis of shigellemia, blood and fecal shigella isolates were compared for serum resistance and siderophore production, and shigellemic patients were examined for decreased serum bactericidal activity or increased serum transferrin saturation compared to control patients with non-bacteremic shigellosis. the majority of both blood (36/38) and fecal (36/48) shigella isolates were sensitive to normal serum (greater than 2 log kill/60 ... | 1990 | 2373088 |
| [retinal artery occlusion: etiology and risk factors apropos of 151 cases]. | a retrospective study covering 151 patients was conducted in order to determine the incidence of risk factors (arterial hypertension, diabetes mellitus, smoking, hyperlipidemia, and cardiovascular disorders) in cases of retinal artery occlusion. occlusion of the central artery was found in 43% of the patients, occlusion of one or several branch arteries in 53%, and cilioretinal artery occlusion in 4%. a monofactorial study of risk factors produced a statistically significant value only for arter ... | 1976 | 2366474 |
| kinetics of "p"-site-mediated inhibition of adenylyl cyclase and the requirements for substrate. | the kinetics of "p"-site-mediated inhibition of adenylyl cyclase was studied with the detergent-solubilized enzyme from rat brain. mn2(+)-activated adenylyl cyclase exhibited typical noncompetitive inhibition by 2'-d3'-amp or 2',5'-dideoxyadenosine (2',5'-ddado). however, enzyme that was preactivated with guanosine 5'-o-(3-thiotriphosphate) (gtp gamma s) or proteolytically with ninhibin (+ gtp gamma s) exhibited apparently uncompetitive inhibition with either 2'-d3'-amp or 2',5'-ddado and with e ... | 1990 | 2365688 |
| [study of the blink reflex in spinocerebellar degeneration]. | blink reflex (br) was composed of r1 and r2. r1 is considered to indicate the brainstem function. the purpose of this study was to evaluate the brainstem function of spinocerebellar degeneration (scd) by the r1 latency (r1). br was evoked by the percutaneous stimulation of supraorbital nerve. and r1 was measured by the action potential of orbucularis oculi muscle. direct response was recorded by the percutaneous stimulation of facial nerve to determine the terminal latency of facial nerve (d). b ... | 2008 | 2364628 |
| [hepato-renal polycystic disease and liver hydatidosis (a rare association)]. | a case is presented of a patient with polycystic kidneys and familial history of this disease who was from a zone of hydatid endemism. the patient had a hepatic mass compatible with hydatid cyst. diagnoses by imaging, like echography and cat, confirmed it, aside from evidencing polycystic liver disease. there was operative and anatomopathologic confirmation of both diseases of the liver. after a review of the bibliography that shows a scant incidence of this concomitance, some remarks are offere ... | 1990 | 2346686 |
| evaluation of children with possible appendicitis using technetium 99m leukocyte scan. | we evaluated the use of technetium 99m albumin colloid white blood cell (tac-wbc) scan in 33 children with possible appendicitis. ten children had appendicitis, four of whom had perforated their appendix at the time of surgery. children with appendicitis differed from children without appendicitis in the incidence of right lower quadrant rebound tenderness (60% vs 17.4%), and white blood cell count (x10(3)/mm3) (14.0 + 1.9 vs 10.7 +/- 1.0). tac-wbc scans were interpreted as either positive, nega ... | 2008 | 2330248 |
| death in shigellosis: incidence and risk factors in hospitalized patients. | the total number of admissions and deaths of patients with shigellosis were ascertained at the dhaka treatment centre of the international centre for diarrhoeal disease research, bangladesh, 1974-1988, and the characteristics of 67 patients who died were compared with those of 134 discharged alive. of 9780 shigella-infected inpatients, 889 (9.1%) died; 32.3% of deaths occurred in children less than 1 year of age. fatality rates were highest (10.3%) in shigella sonnei-infected patients and lowest ... | 1990 | 2313128 |
| enzyme-linked immunosorbent assay for shiga toxin and shiga-like toxin ii using p1 glycoprotein from hydatid cysts. | shiga toxin from shigella dysenteriae type 1 strains and shiga-like toxins (slt) i and ii from escherichia coli bind to terminal alpha-d-galp-(1----4)-d-galp containing glycolipids. hydatid cyst fluid isolated from sheep infected with echinococcus granulosus contains a glycoprotein (p1gp) with a terminal alpha-d-galp-(1----4)-d-galp disaccharide. preparations of p1gp were shown to interact directly with shiga toxin and to inhibit the binding and cytotoxicity of shiga toxin to hela cells. a sandw ... | 1990 | 2295845 |
| [supernumerary teeth in the deciduous dentition]. | the authors presented sovrannumeraries (s) teeth in primary dentition, and the pedodontic, surgical and orthodontic problems. semeiotics signs, and x ray suggested, are described. all the types of s. teeth are showed: supplementaries, conoides, tuberculates and infundibuliformes. for every type characteristics, problems and surgical timing are described. tuberculates and infundibuliformes teeth are the most dangerous for the permanent teeth interrupted: eruption delay, dilacerations, impactions, ... | 1991 | 2288972 |
| polysplenia in a fetus with bradycardia from 26 to 36 weeks' gestation, complex cardiac malformations, and heart block. | polysplenia is one of several splenic anomalies. frequently, as in the case presented here, significant cardiac anomalies coexist. these cardiac malformations determine the clinical course. in this case, early death was expected and occurred. this patient is different from others described in the literature in that persistent fetal bradycardia was noted at 26 weeks' gestation. | 2008 | 2276938 |
| antenatal diagnosis of parenti-fraccaro type achondrogenesis. | 2008 | 2276781 | |
| in vitro diffusion of lidocaine across endotracheal tube cuffs. | smooth emergence from general endotracheal anesthesia is frequently complicated by coughing induced by stimulation from an endotracheal tube. lidocaine and other local anesthetics have been shown to anesthetize important rapidly adpating stretch receptors in the dog trachea. with the aim of providing a reservoir for continuous lidocaine release to adjacent tracheal tissue, we examined the ability of clinically used concentrations of lidocaine to diffuse across a commonly used endotracheal tube c ... | 1990 | 2275911 |
| in vitro diffusion of lidocaine across endotracheal tube cuffs. | smooth emergence from general endotracheal anesthesia is frequently complicated by coughing induced by stimulation from an endotracheal tube. lidocaine and other local anesthetics have been shown to anesthetize important rapidly adpating stretch receptors in the dog trachea. with the aim of providing a reservoir for continuous lidocaine release to adjacent tracheal tissue, we examined the ability of clinically used concentrations of lidocaine to diffuse across a commonly used endotracheal tube c ... | 1990 | 2275911 |
| [role of protons and bicarbonate in adaptative gastric cytoprotection]. | the unknown mechanism of adaptative gastric cytoprotection (agc) induced by 20% ethanol and subsequent injury with 70% ethanol was studied in wistar rats. pretreatment with indomethacin or hgii2 did not prevent the agc, there suggesting that neither endogenous pgs nor gastric mucus take part in its mechanism. on the other hand, ranitidine pretreatment blocked and even aggravated the damage induced by ethanol-ethanol. in contrast, the latter phenomenon was reverted by 20% acidified ethanol. it is ... | 1990 | 2275313 |
| bovine spongiform encephalopathy (bse): menace or mirage? | 1990 | 2271904 | |
| the physiological and psychological effects of the expression and inhibition of emotion. | previous work has indicated that humor enhances immunity, but the immunological effects of overt crying have not been studied. thirty-nine women viewed sad and humorous videotapes and either inhibited or expressed overt expressions of laughter and weeping; these were observed and timed. moods and immunity (s-iga) were repeatedly measured. the humorous stimulus resulted in improved immunity, regardless of the overt laughter expressed; overt crying was immunosuppressive, whereas the inhibition of ... | 1990 | 2271804 |
| haematological investigation in neonates. | 1990 | 2271791 | |
| fatty streak development in the vicinity of aortic coarctations in hypercholesterolemic rabbits. | the influence of altered local hemodynamics on fatty streak development in rabbits fed high cholesterol diets was investigated. an aortic coarctation was created in the abdominal aorta of nine rabbits by placing a partially constricting gold or silver band (1.7 mm x 10 mm) around the aorta between the renal arteries and aortic bifurcation. controls were 20 rabbits; seven sham operated and 13 unoperated rabbits. the abdominal aorta 1-2 cm proximal to the coarctation showed lipid deposition involv ... | 1990 | 2271758 |
| independent eye movements in the turtle. | in order to evaluate the normal eye movements of the turtle, pseudemys scripta elegans, the positions of each eye were recorded simultaneously using two search-coil contact lenses. optokinetic nystagmus (okn) was strikingly unyoked in this animal such that one eye's slow-phase velocity was substantially independent of that of the other eye. on the other hand, the fast-phase motions of both eyes occurred more or less in synchrony. an eye's slow-phase gain is primarily dependent on the direction a ... | 1994 | 2271458 |
| critical care medicine: activities, advances, and implications for infection control. | 2008 | 2271151 | |
| survey of enteropathogenic agents in children with and without diarrhoea in ghana. | a survey was carried out over 1 year in a rural area of ghana on the isolation, detection and/or identification of enteric pathogens from children under 5 years of age with and without diarrhoea. the isolation and detection rate of shigella flexneri, shigella dysenteriae, giardia lamblia and rotavirus were higher in children with diarrhoea than in controls. yersinia enterocolitica, vibrio cholerae and vibrio parahaemolyticus were not isolated during the period of this survey. the incidence of ot ... | 1990 | 2270006 |