Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| recombinant polypeptide from the gp48 region of the bovine viral diarrhea virus (bvdv) detects serum antibodies in vaccinated and infected cattle. | to characterize the immune response of cattle to bovine viral diarrhea virus (bvdv) glycoprotein gp48, we have produced a large amount of recombinant glutathione-s-transferase-gp48 (gst-gp48) fusion protein in escherichia coli. antibodies to gp48 were present in cattle vaccinated with killed or modified-live virus vaccination, or following natural infection. these results were in agreement with results of serum neutralization (sn) test which detected gp53 of bvdv. | 1992 | 1333674 |
| protein phosphatase 2a is a specific protamine-kinase-inactivating phosphatase. | purified preparations of a protamine protein kinase from bovine kidney cytosol [damuni, amick & sneed (1989) j. biol. chem. 264, 6412-6416] were inactivated after incubation with near-homogeneous preparations of protein phosphatase 2a1 and protein phosphatase 2a2. these protein phosphatase 2a-mediated inactivations of the protamine kinase were unaffected by highly purified preparations of inhibitor 2, but were prevented when the incubations were performed in the presence of 100 nm microcystin-lr ... | 1992 | 1332680 |
| expression and purification of growth hormone-releasing factor with the aid of dihydrofolate reductase handle. | expression of a fusion protein composed of dihydrofolate reductase and a derivative of growth hormone-releasing factor resulted in the formation of inclusion bodies in escherichia coli at 37 degrees c. among various chemicals, such as detergents, protein denaturants, and acetic acid, tested for the ability to dissolve the inclusion bodies, acetic acid, brij-35, deoxycholic acid sodium salts, guanidine-hcl, and urea showed a strong solubilizing effect without damaging the dhfr activity. acetic ac ... | 1992 | 1331037 |
| a case-control study of selected pathogens including verocytotoxigenic escherichia coli in calf diarrhea on an ontario veal farm. | a case-control study of diarrheal disease in veal calves was conducted over a three month period on a single large veal farm in southern ontario. one hundred diarrheic calves (cases) were identified by visual examination of their feces. each case was matched to two nondiarrhetic controls from the same room on the same day, and a fecal sample was obtained from each animal. fecal consistency of cases and controls was observed daily for one week following sample collection. control calves which dev ... | 1992 | 1330276 |
| structural elements required for the cooperative binding of the herpes simplex virus origin binding protein to oris reside in the n-terminal part of the protein. | the origin binding protein (obp) of herpes simplex virus type 1 is required to activate a viral origin of replication in vivo. we have used intact obp as well as a truncated form of the protein expressed in escherichia coli to investigate the protein-protein interactions, as well as the protein-dna interactions involved in the formation of a nucleoprotein complex at a viral origin of replication (oris) in vitro. the salient findings demonstrate that the n-terminal part of obp is required for the ... | 1992 | 1324937 |
| organella-targeted expression of rat liver cytochrome p450c27 in yeast. genetically engineered alteration of mitochondrial p450 into a microsomal form creates a novel functional electron transport chain. | a modified rat cytochrome p450c27, whose mitochondrial targeting signal had been replaced by a possible microsomal targeting signal of bovine cytochrome p450c17, was expressed in yeast. the modified p450c27 hemoprotein was correctly localized on yeast microsomes and exhibited the p450c27-dependent monooxygenase activity by addition of bovine adrenodoxin (adx) and nadph-adrenodoxin reductase (adr). considering the previous observation that p450c27 with its own mitochondrial targeting signal was i ... | 1992 | 1322905 |
| in vitro isoprenylation and membrane association of mouse rod photoreceptor cgmp phosphodiesterase alpha and beta subunits expressed in bacteria. | we investigated the specificity of caax box-related isoprenylation of rod photoreceptor cgmp phosphodiesterase (pde) subunits expressed in bacteria and the consequences of this modification on rod disk membrane association. full-length cdna sequences of the alpha and beta subunits of mouse pde, inserted into bacterial pet expression vectors, were overexpressed as fusion proteins containing 28 (bmp-alpha) and 26 (bmp-beta) additional amino acid residues at their n termini. both fusion proteins we ... | 1992 | 1314827 |
| equilibrium, kinetic, and footprinting studies of the tus-ter protein-dna interaction. | arrest of dna replication in the terminus region of the escherichia coli chromosome is mediated by protein-dna complexes composed of the tus protein and 23 base pair sequences generically called ter sites. we have characterized the in vitro binding of purified tus protein to a 37-base pair oligodeoxyribonucleotide containing the terb sequence. the measured equilibrium binding constant (kd) for the chromosomal terb site in kg buffer (50 mm tris-cl, 150 mm potassium glutamate, 25 degrees c, ph 7.5 ... | 1992 | 1313800 |
| production and characterization of recombinant insulin-like growth factor-i (igf-i) and potent analogues of igf-i, with gly or arg substituted for glu3, following their expression in escherichia coli as fusion proteins. | the development of an efficient expression system for insulin-like growth factor-i (igf-i) in escherichia coli as a fusion protein is described. the fusion protein consists of an n-terminal extension made up of the first 46 amino acids of methionyl porcine gh ([met1]-pgh) followed by the dipeptide val-asn. the latter two residues provide a unique hydroxylamine-sensitive link between [met1]-pgh(1-46) and the n-terminal gly of igf-i. downstream processing of the fusion proteins involved isolation ... | 1992 | 1311930 |
| functional reconstitution of membrane proteins in monolayer liposomes from bipolar lipids of sulfolobus acidocaldarius. | membranes of sulfolobus acidocaldarius, an extreme thermophilic archaebacterium, are composed of unusual bipolar lipids. they consist of macrocyclic tetraethers with two polar heads linked by two hydrophobic c40 phytanyl chains which are thought to be arranged as a monolayer in the cytoplasmic membrane. fractionation of a total lipid-extract from s. acidocaldarius yielded a lipid fraction which forms closed and stable unilamellar liposomes in aqueous media. beef heart cytochrome c-oxidase could ... | 1992 | 1309769 |
| molecular characterization of cdna encoding for adenylate kinase of rice (oryza sativa l.). | two types of genes (adk-a, and adk-b) encoding for adenylate kinase (ak, ec 2.7.4.3.) were isolated from the cdna library constructed from poly(a)+ rna of rice (oryza sativa l.). two cdnas were heterogeneous at 5' and 3' ends of non-coding sequences and had possible polyadenylation signals. one of the genes, adk-a, had 1154 bp sequences encoding 241 amino acid residues, while the other type, adk-b, contained 1085 bp sequences encoding for 243 amino acid residues. homology between adk-a and adk-b ... | 1992 | 1302636 |
| acute coliform mastitis in dairy cows: endotoxin and biochemical changes in plasma and colony-forming units in milk. | 1992 | 1300679 | |
| the biological interaction of cis- and trans-urocanic acid and dna. | the potential for the cis and trans isomers of urocanic acid to produce dna damage was measured by assays for dna binding (32p-postlabeling assay), for induction of dna repair (unscheduled dna synthesis assay) and induction of mutations (salmonella typhimurium and escherichia coli plate-incorporation assays). these assays did not detect any evidence of a direct effect of either isomer of urocanic acid on dna over a wide range of concentrations. these results suggest that neither isomer of urocan ... | 1992 | 1300140 |
| [detection of verotoxin-producing escherichia coli using polymerase chain reaction from dairy cattle]. | the vero cytotoxin (vt) is responsible for hemorrhagic colitis and hemolytic uremic syndrome. polymerase chain reaction (pcr) was used to detect vt-producing coliform bacteria from dairy cattle. it was found that 39 (33.3%) of the 117 fecal samples examined were recognized with vt genes in bglb enrichment broth by the pcr method (named bglb-pcr). of the vt-positive samples, 31 samples (26.5%) were found to have vt-producing escherichia coli. frequencies of isolation in younger cattle (under 5 mo ... | 1992 | 1293218 |
| escherichia coli f0f1-atpase. residues involved in catalysis and coupling. | the molecular biological approach has provided important information toward understanding the complexities of the f0f1 atpase. this article focuses on our recent results on the atpase catalytic site contained in the beta subunit and the role of the gamma subunit in regulation of proton transport. we used a combination of affinity labeling and mutagenesis to locate several residues of the alpha and beta subunits in the catalytic site. adenosine triphosphopyridoxal (ap3-pl) labeled beta lys-155, b ... | 1992 | 1288330 |
| expression of the phosphorylase kinase gamma subunit catalytic domain in escherichia coli. | the catalytic subunit of phosphorylase b kinase (gamma) and an engineered truncated form (gamma-trc, residues 1-297) have been expressed in escherichia coli. the truncated protein included the entire catalytic domain as defined by sequence alignment with other protein kinases but lacked the putative calmodulin binding domain. full-length protein was produced in insoluble aggregates. some activity was regenerated by solubilization in urea and dilution into renaturating buffer but the activity was ... | 1992 | 1287663 |
| identification of inducible calmodulin-dependent nitric oxide synthase in the liver of rats. | a calmodulin-dependent nitric oxide synthase was significantly induced in the liver of rats treated intravenously with heat-killed propionibacterium acnes and 5 days later with escherichia coli lipopolysaccharide. the apparent calmodulin-dependent and -independent isozymes were separated by mono q column chromatography after their partial purification by 2',5'-adp-agarose affinity chromatography. both enzymes had a molecular weight of 125,000 as determined by sds-polyacrylamide gel electrophores ... | 1992 | 1281157 |
| recombinant hiv-1 nucleocapsid protein p15 produced as a fusion protein with glutathione s-transferase in escherichia coli mediates dimerization and enhances reverse transcription of retroviral rna. | human immunodeficiency virus 1 (hiv-1) nucleocapsid protein p15 was produced as a fusion protein with glutathione s-transferase (gst) in escherichia coli. rapid purification of gst::p15 in an active form by one-step glutathione-agarose chromatography was accomplished in the presence of an antioxidant. recombinant p15 fused to gst was shown to stimulate the dimerization of viral rna. hiv-1 reverse transcriptase-catalyzed in vitro synthesis of minus-strand cdna from synthetic human trna(lys3uuu) a ... | 1992 | 1280240 |
| modification of resistance of mice to naegleria fowleri infections. | naegleria fowleri, which produces a fatal meningoencephalitis in humans, is also able to produce a progressive and fatal disease in mice. the course of the disease in dub/icr mice is dependent upon the infecting dose of organisms, whether administered intraperitoneally (i.p.) or intravenously (i.v.). all of the mice receiving 10(7) trophozoites/mouse i.v. or 4.85 x 10(7) trophozoites/mouse i.p. were killed within 10 days. escherichia coli o26:b6 lipopolysaccharide, administered at a dose of 1 mg ... | 1976 | 1270145 |
| studies on the binding of 5,5-diphenyl-hydantoin to nucleic acids in vitro and to rat brain subcellular fractions in vitro and in vivo. | the binding of 5,5-diphenylhydantoin (dph) to nucleic acids (bovine brain rna, rat liver ribosomal and trna, torula utilis rna, and calf thymus and escherichia coli dna was studied using ultraviolet spectroscopy, gel chromatography and thermal transition profiles. within the sensitivity of these methods, it was found that there is essentially little or no interaction between dph and nucleic acids in vitro as has been reported previously. little, if any evidence of dph intercalation with dna was ... | 1976 | 1255490 |
| diverse effects of bursectomy on humoral immune responses in the chicken. | chickens bursectomized hormonally or surgically at various ages in embryonic to postembryonic lives were immunized with a mixture of antigens, and the antibodies produced titrated. immune responses to salmonella pullorum (sp), brucella abortus (ba). staphylococcus aureus (staph) and ovalbumin (oa) were more markedly suppressed by the bursal deprivation than those to heterogenous erythrocytes, viruses and such bacteria as pasteurella multocida (pm) and escherichia coli b. the immune response to b ... | 1975 | 1187514 |
| [sodium thiocyanate as a protective agent in intensive calf fattening. brief report]. | daily administration of sodium thiocyanate at 750 mg a calf for the first three weeks in the fattening stalls reduced the occurrence of illness by 60%. this protective effect seemed to persist during the following weeks without additional treatment. addition of the thiocyanate to the food may be beneficial through a nutritive effect. | 1975 | 1106349 |
| hematology of the neonatal calf. ii. response associated with acute enteric infections, gram-negative septicemia, and experimental endotoxemia. | the hematological responses of neonatal calves with acute enteric infections were compared to the responses of calves with septicemia and experimentally induced endotoxemia. the mean hematocrit of septicemic calves (45.0% +/- 7.8) was similar to that of calves with primary enteric infections (45.3% +/- 7.0) but the total plasma protein concentration of septicemic calves (5.8g/100 ml +/- 0.69) was significantly lower than that of calves with primary enteric infections (8.6 g/100 ml +/- 1.5). the ... | 1975 | 1104259 |
| distribution of r plasmids among the o-antigen types of escherichia coli isolated from human and animal sources. | the o-antigen types of 600 independently isolated escherichia coli strains from human feces have been determined, and the types have been related to the antibiotic resistance patterns of the strains. the relative abundance of each o-antigen type differed in the susceptible and resistant series of strains. the majority (86%) of the resistant strains carried r plasmids. resistant e. coli (20.3%) were found associated with o-antigen types 8, 9 and 101, whereas the susceptible strains covered a wide ... | 1975 | 1101815 |
| the effects of organic solvents on escherichia coli dna polymerase iii. | the polymerizing ability of escherichia coli dna polymerase iii is enhanced by a variety of water-miscible organic solvents of which dimethyl sulfoxide at 17% (v/v) is the most effective tested. the extent of stimulation depends on the organic solvent used and its concentration, but shows no obvious correlation with the chemical structure of the solvent or its dielectric constant. kinetic studies indicate that the mechanism of stimulation is complex. | 1975 | 1098695 |
| the effects of overloading in density-gradient centrifugation. | the effects of overloading of the sample zone in density gradient centrifugation have been studied by use of a three-component shelf-lavered sample in which the total protein concentration was increased by addition of different amounts of albumin. it is found that overloading of the gradient gives rise to particle movements which are not predictable from the svedberg equation. the two typical effects of overloading are dislocation of the zone mass centres and changes in the zone shapes. it is fo ... | 1975 | 1097246 |
| [problems of resistance (author's transl)]. | the results of sensitivity tests performed during the period from 1970 up to 1973 inclusive are compared with those obtained in pervious years. this showed that resistance to penicillin had increased in staphylococci causing bovine mastitis. during each single year, however, there was a decrease. s. typhimurium isolated from cattle showed increased resistance to chloramphenicol, neomycin and ampicillin; s. dublin showed an increase in resistance to chloramphenicol. e. coli isolated from calves w ... | 1975 | 1096359 |
| some effects of calcium and magnesium ions on the activity of bovine pancreatic deoxyribonuclease a. | bovine pancreatic deoxyribonuclease requires divalent metal cations for hydrolysis of dna. the effects of calcium and magnesium, alone and combined, on the rate and kinetics of the reaction were examined. divalent metal salts of dna were used as substrates. the ratio of either ca-2+ or mg-2+ to dna-p in these salts was 1:2. the mg-2+ salt of dna was found to have sufficient mg-2+ for optimal dnaase activity. addition of mgcl-2 to a large excess of mg-2+ over dna-p had no effect on the rate. km f ... | 1975 | 1095074 |
| quantitation of amanitins in amanita verna with calf thymus rna polymerase b. | a procedure utilizing the specific inhibition of calf thymus dna-directed rna polymerase b has been applied to the quantitation of amanitins. this procedure has permitted the accurate quantitation of alpha-amanitin in amounts as low as 0.05 nanogram, a sensitivity 2000-fold greater than chemical detection methods used following tlc. analysis of extracts of specimens of amanita verna identified by morphological criteria has demonstrated that while toxin concentration is variable, some specimens ... | 1975 | 1094215 |
| enzymatic multiplication of a chemically synthesized dna fragment. | a synthetic dna fragment of 19 residues was enlarged by the enzymatic addition of deoxyadenylate residues to its 3'-end with calf thymus terminal deoxynucleotidyl transferase. the 3'-terminus of this elongated dna strand was blocked with 2', 3'-dideoxyadenylate to prevent hydrolysis by the 3'-exonuclease function of e. coli dna polymerase i. this elongated and 3'-blocked fragment was annealed to an oligomeric primer and used as a template for the synthesis of a complementary copy of the syntheti ... | 1975 | 1093143 |
| defective mononuclear leukocyte chemotaxis in the chediak-higashi syndrome of humans, mink, and cattle. | chemotaxis of mononuclear leukocytes from humans, mink, and cattle was evaluated in vitro using a morphologic boyden chamber technique and a new 51-cr-labeled mononuclear radioassay with a double micropore filter system. significantly decreased mononuclear leukocyte chemotactic response were noted when human, mink, or cattle chediak-higashi cells were tested using autologous serum or endotoxin-activated autolotous serum. a similar chediak-higashi mononuclear leukocyte defect was noted in humans ... | 1975 | 1092386 |
| enzymatic arginylation of beta-melanocyte-stimulating hormone and of angiotensin ii. | porcine beta-melanocyte-stimulating hormone and angiotensin ii were examined as acceptors in the reaction catalyzed by arginyl-trna-protein transferase. both inhibited enzymatic transfer of [14c]arginine from trna to bovine albumin. inhibition was competitive with albumin and the k-i values were, respectively, 15 and 0.8 mum. the expected arginylated compounds were isolated and characterized. beta-melanocyte-stimulating hormone and its arginylated product had identical activities in the frog epi ... | 1975 | 1091639 |
| deoxyribonucleic acid-cytosine methylation by host- and plasmid-controlled enzymes. | deoxyribonucleic acid (dna)-cytosine methylation specified by the wild-type escherichia coli k 12 mec+ gene and by the n-3 drug resistance (r) factor was studied in vivo and in vitro. phage lambda and fd were propagated in the presence of l-[methyl-3h]methionine in various host bacteria. the in vivo labeled dna was isolated from purified phage and depurinated by formic acid-diphenylamine treatment. the resulting pyrimidine oligonucleotide tracts were separated according to size and base composit ... | 1975 | 1091619 |
| [bacteriocinogeny in yersinia enterocolitica]. | bacteriocinogeny was found in 19.8 per cent of y. enterocolitica strains tested. it was designated by the authors as enterocoliticacinogeny and the substance produced by the pathogen was named enterocoliticacine. bacteriocinogeny was found among the collection strains isolated in the south primorye and not among the strains isolated abroad. some cultures of y. enterocolitica isolated abroad and in the south primorye were used as indicator strains for revealing enterocoliticacinogeny. enterocolit ... | 1975 | 1091204 |
| calf ligated intestinal segment test to detect enterotoxigenic escherichia coli. | the ligated intestinal segment test in the young calf was utilized to establish the enterotoxigenicity of approximately 600 escherichia coli isolates obtained from fecal specimens intestinal contents of calves with diarrheal disease. one hundred and fifty isolates were routinely tested in a single calf. there was little problem with false-positive reactions. false-negative reactions normally occurred only if the isolate was tested in the posterior 3 m of the small intestine. the ligated small in ... | 1975 | 1090536 |
| inhibition of escherichia coli by bovine colostrum and post-colostral milk. i. complement-mediated bactericidal activity of antibodies to a serum susceptible strain of e. coli of the serotype o 111. | bovine colostral whey diulted in unheated bovine milk or in kolmer saline containing 5 per cent pre-colostral calf serum was bactericidal for escherichia coli nctc 9703 (serotype o 111). whey diluted in saline without pre-colostral calf serum was inactive. bactericidal activity was abolished by heating at 56 degrees for 30 minutes or by pre-incubating with n-acetyl-l-tyrosine ethylester, indicating that complement present in milk or pre-colostral calf serum was involved in the bactericidal activ ... | 1975 | 1090521 |
| indirect consequences of low-level use of antimicrobial agents in animal feeds. | antibiotics may be classified as "therapeutic" or "feed" depending on their use for treatment of disease or for promotion of growth in animals. the mechanism of growth promotion is largely unknown, but it has been shown that the specific feed additive causes defined lesions in the cell wall of escherichia coli. this effect may sensitize the bacteria to the action of therapeutic drugs. damage to the bacterial cell wall may also cause the bacteria to be more susceptible to the body defense mechani ... | 1975 | 1090454 |
| specific binding of a nonhistone chromosomal protein from lymphocyte to dna. | the isolation of a nonhistone chromosomal protein, nh30 000, from bovine lymphocytes by affinity chromatography on a dna-agarose column is described. the procedure starts with nonhistone fraction nh4 obtained by hydroxyapatite chromatography as described previously [blüthmann, h., mrozek, s. & gierer, a. (1975) eur. j. biochem. 58, 315-326]. protein nh30 000 migrates as a single band with a molecular weight of 30 000 on sodium dodecylsulfate polyacrylamide gels. it contains, on a molar basis 25. ... | 1976 | 1009927 |
| bronchiectasis in yearling feedlot cattle. | thoughout all of 1974, we surveyed, for illnesses and deaths, about 407,000 yearling feedlot cattle. from the 3,943 dead cattle, 1,988 necropsies were made; of the cattle necropsied, 32 (1.6%) had bronchiectasis. in this disease, the permanently dilated small bronchi and bronchioles, located in ventral parts of the lungs, were filled with accumulations of exudate and microorganisms, including pasteurella hemolytica, pasteurella multocida, corynebacterium pyogenes, escherichia coli, salmonella an ... | 1976 | 956033 |
| tissue distribution and residues of beta-lactam antibiotics in normal dairy cows. | tissue residues and concentrations of benzylpenicillin, cloxacillin, ampicillin, amoxycillin, cephapirin, and cephacetrile were determined in normal dairy cows after parenteral administration of several forms of these drugs. assay methods included the sarcina lutea kidney test of van schothorst, the bacillus subtilis bga tests at ph 6.0 and 8.0, the escherichia coli test and a sarcina lutea test performed at ph 8.0 of the agar, and specific quantitative assay methods. the e. coli test method dem ... | 1977 | 918925 |
| [methionyl-trna synthetase from wheat embryo: dissociation into subunits (author's transl)]. | wheat -embryo methionyl-trna synthetase is a dimeric protein of beta2 structure. when highly diluted, it loses the capacity to catalyze atp-[32p]ppi exchange and to aminoacylate trnamet: at low enzymatic concentrations the rates of formation of[32p]atp and [14c]methionyl-trnamet are lower than those predicatedby extrapolating the rates determined at higher enzyme concentrations. the difference between observed and expected rates becomes greater with decreasing enzyme concentration. filtration of ... | 1977 | 849749 |
| secondary-structure predictions of calcium-binding proteins. | the known tertiary structure of carp muscle parvalbumin is consistent with an "ef-hand" architecture (helix-loop-helix) for each calcium-ion binding site. primary-sequence alignments have indicated four ef hands in rabbit skeletal muscle troponin c and in rabbit myosin alkali light chains. five secondary-structure prediction methods, based on amino acid sequence only, have been fully computerized and used to calculate joint prediction histograms for several calcium-binding proteins. the joint hi ... | 1977 | 836806 |
| [etiology of mastitidos in dairy cows in slovakia during 1975]. | in 1975, in the veterinary institutes in slovakia bacteriological examinations of 54824 samples of milk coming from 28737 dairy cows were performed. bacterial germs responsible for the inflammation of the mammary gland were found in the milk of 18.91% of cows. these were: streptococcus agalactiae (in 13.93% of cows), staphylococcus aureus (2.86%), other streptococci (1.32%), e. coli (0.13%), klebsiella sp. (0.13%), corynebacterium pyogenes (0.15%), other bacterial germs (0.39%). streptococci and ... | 1976 | 828997 |
| [infective situation in a cow barn contaminated with klebsiella mastitis]. | the infection situation in a four-row cow-house for 153 animals with a frequent occurrence of klebsiella mastitis was subject to a detailed analysis. the following results were obtained after two collections of blood and udder-quarter milk samples examined by the test-tube agglutination, gel precipitation, and bacteriological diagnosis methods: 1. streptococcus agalactiae was isolated from milk samples 28 and 31 times, pseudomonas aeruginosa 15 and 19 times, staphylococcus arueus 16 and 19 times ... | 1976 | 828991 |
| reversibility of partial denaturation of dna. | the hypochromicity recovery of a partially denatured dna sample when salt concentration is suddenly increased at an intermediate stage of the transition, is studied. the results of csc1 gradient analysis, polyethylenglycol/dextran partition analysis, and the behaviour in a new thermal transition, support the view that the process is an intramolecular double chain renaturation as that induced by cooling. the degree of denaturation irreversibility is dependent on the size of dna segments between t ... | 1976 | 825148 |
| ]acute severe mastitis in cows of the dutch-friesian breed (author's transl)]. | one hundred cases of severe acute mastitis are reported. bacteriological examination of the milk was negative in 23% of the cases. e. coli was most frequently found to be the causative organism (23%), followed by st. aureus (19%), c. pyogenes (10%), str. dysgalactiae (9%), str. agalactiae (5%) and a number of other pathogenic agents such as atypical streptococci, str. uberis, haemolytic streptococci, aerobacter aerogenes, kl. pneumoniae, a clostridium and p. multocida. in several cases, a tentat ... | 1976 | 818734 |
| [etiology of mastitis in dairy cows in slovakia during 1972-1974]. | in the period from 1972 to 1974, veterinary institutes in slovakia performed bacteriological examinations in 178,853 milk samples coming from 72,454 dairy cows. on an annual average, the number of examined samples was higher by 61% than in 1967-1971. germs responsible for the inflammation of the mammary gland were found in the milk of 22.32% of the dairy cows. the following bacterial germs were represented: streptococcus agalactiae - 15.77%, staphylococcus aureus - 4.19%, other streptococci - 1. ... | 1975 | 814671 |
| bactericidal and haemolytic activity of complement in bovine colostrum and serum: effect of proteolytic enzymes and ethylene glycol tetraacetic acid (egta). | bovine colostral whey (cw) possessed heat-labile bactericidal activity against an enteropathogenic serum-susceptible strain of escherichia coli. activity was readily destroyed by trypsin or rat pancreatic juice, and less readily by chymotrypsin. loss of activity due to trypsin, but not by chymotrypsin, could be prevented by addition of excess bovine colostral trypsin inhibitor. a second enteropathogenic strain of e. coli was killed by cw only if exogenous complement was added. this activity was ... | 1975 | 813560 |
| [etiology of dairy cows mastitis in slovakia in the years 1972-1974]. | in the period from 1972 to 1974, veterinary institutes in slovakia performed bacteriological examinations in 178,853 milk samples coming from 72,454 dairy cows. on an annual average, the number of examined samples were higher by 61% than in 1967-1971. germs responsible for the inflammation of the mammary gland were found in the milk of 22.32% of the dairy cows. the following bacterial germs were represented: streptococcus agalactiae -- 15.77%, staphylococcus aureus -- 4.19%, other streptococci ... | 1975 | 813355 |
| endonuclease ii of escherichia coli: dna reacted with 7-bromomethyl-12-methylbenz[alpha]anthracene as a substrate. | an endonuclease ii preparation from escherichia coli makes single strand breaks in dna which has been treated with the carcinogen 7-bromomethyl-12-methylbenz[alpha]anthracene. in addition, the enzyme preparation excises n6-(12-methylbenz[alpha]anthracenyl-7-methyl)adenine and n2-(12-methylbenz[alpha]anthracenyl-7-methyl)guanine residues from the dna. these are relased as the modified purine bases, not as purine nucleoside derivatives. the rate of release of the adenine derivative is three to fo ... | 1975 | 812550 |
| [comparative study of some quaternary ammoniums neutralizing agents. influence of the nature of the bacterial strains used]. | the neutralising capacity of the anti-bacterial activity of a quaternary ammonium salt, depends on the nature of the bacterial strains used. consequently, the choice of a neutralising agent of the bactericidal activity can only be made following a preliminary study on several bacterial species. out of five products which we tested, we found the tween-lecithin fresh egg mixture alone had satisfactory activity. | 1975 | 812041 |
| selective adhesion of microorganisms to the ductular epithelium of the bovine mammary gland. | streptococcus agalactiae, streptococcus faecalis, staphylococcus aureus, escherichia coli, and corynebacterium bovis were examined for their ability to adhere to the ductular epithelial cells of the bovine udder. s. agalactiae and s. aureus adhered readily and in large numbers, whereas the other organisms adhered poorly or not at all. the organisms showing the ability to adhere are those which frequently cause mastitis. these data suggest that selective adherence to the ductular epithelium may b ... | 1975 | 811566 |
| [false-positive results obtained on examining slaughtered animals for the presence of antibiotic residues (author's transl)]. | as part of the examination of emergency-slaughtered animals for the presence of antibiotic residues, studies were done to see whether false-positive results would be obtained when the sarcina lutea kidney test and bacillus subtilis bga test were performed. when the s. lutea kidney test was positive in cattle, calves and swine, penicillin was invariably found to be present in those animals, the histories of which showed that they had not been given antibiotics. a syringe and an injected fluid con ... | 1975 | 809859 |
| [effect of long-term freezing preservation on the level of bacterial contamination of the sperm]. | fourty-five ejaculates of breeding bulls were examined at a breeding station in order to study the contamination level of sperm after ejaculation, after thinning, after finished equilibration, after freezing, and after three months after placement in liquid nitrogen at -- 196 degrees c. the amount of germs in sperm was found to increase rapidly in the course of examination and thinning at laboratory temperature. the average number of 4,149 germs increased to 9,729. during equilibration the numbe ... | 1975 | 808013 |
| teat apex coliform populations and coliform mastitis--a herd study. | a dairy herd having a high incidence of coliform mastitis was observed by means of pre- and post-milking samples and teat apex culture. infected quarters were the major source of teat apex contamination with coliform bacteria and therefore constituted a risk to unifected quarters and to other cows. coliform populations on the apex from environmental sources appeared to be transitory. | 1975 | 806418 |
| herd studies on coliform mastitis. | escherichia coli, enterobacter aerogenes, and klebsiella pneumoniae were responsible for 63, 10, and 11 percent, respectively, of 158 coliform organisms recovered from mastitis cases in 8 california herds. the severity and nature of the coliform mastitis problem were found to vary greatly among herds but were characteristic for each of 4 herds studied in detail. | 1975 | 804464 |
| the effect of leukocyte hydrolases on bacteria. iii. bacteriolysis induced by extracts of different leukocyte populations and the inhibition of lysis by macromolecular substances. | the lysis of 14c-labeled bacteria by hydrolases of human and rabbit leukocytes was studied in vitro. while staphylococcus albus, streptococcus faecalis, and streptococcus mutans were highly susceptible to lysis, staphylococcus auresus was intermediate in its susecptibility to lysis by the leukocyte enzymes. group a streptococcus, listeria monocytogenes, shigella flexneri, escherichia coli, and mycobacterium smegmatis were very resistant to degradation by these enzymes. the lytic activity of leuk ... | 1975 | 804017 |
| leukocyte inhibiting factor (lif) production from human lymphocytes stimulated by bacterial lipopolysaccharides. | leukocyte inhibiting factor (lif) release from lymphocytes cultures stimulated by bacterial lipopolysaccharides (lps) has been investigated. human lymphocytes cultured in the presence of e, coli or s. enteritidis lps were able to release in the supernatants a substance with the following properties: a) same elution pattern of bovine serum albumin (mw 69000) when filtered on sephadex g-100; b) it migrates on disc electrophoresis a an albumin; c) not dialyzable; d) not cytotoxic; e) inhibition of ... | 1976 | 798596 |
| [indices of bacteriological purity of milk obtained in cowsheds during mastitis controla1]. | 1976 | 797599 | |
| cloning of calf thymus satellite i dna in escherichia coli. | the 1400 base pair repeat produced by digestion of calf satellite i dna (phi = 1.714 g/cm3) with ecori, was cloned in e. coli. the hybrid plasmid (pgm 214) which contains the cole1-ap vector (psf 2124) and the 1400 base pair fragment replicates stably in e. coli and can be amplified by chloramphenicol treatment. no clone was found in which more than one "repeat unit" of the satellite i dna was present in the chimaera plasmid. digestion of the original satellite i and the plasmid pgm 214 with r-s ... | 1976 | 796669 |
| coliform contaminated bedding and new infections. | ten holstein cows were bedded on fresh, uncured sawdust seeded with coliform broth culture. escherichia coli concentrations were maintained at 10(6) colony-forming units/g for the 4-wk experimental period. ten control cows were bedded with dry shavings. the elevated bacterial contamination caused an increase in contamination of teat ends; however, no new coliform infections occurred. | 1976 | 795819 |
| characterization of enterotoxigenic bovine escherichia coli. | among 300 isolates of bovine escherichia coli, 56 which had been found enterotoxigenic in calf gut loops were characterized on the basis of o and k antigens, colonial morphology and resistance to seven antimicrobial drugs. the 56 isolates enterotoxigenic in the calf were compared with the nonenterotoxigenic ones. of the 56 enterotoxigenic e. coli the majority possessed the a type of k antigen and had ok groups, o9:k(ps274) or o101:k(rvc118). fourteen of these isolates had the k99 antigen. none o ... | 1976 | 793694 |
| pathological and microbiological observations made on spontaneous cases of acute neonatal calf diarrhea. | the purpose of this report is to describe clinical signs, gross and microscopic lesions, bacteriological and immunofluorescence observations made on spontaneous cases of acute neonatal calf diarrhea (ncd) in dairy and beef herds. the following diagnostic tools were used: 1) direct smears of intestinal content, 2) escherichia coli counts, 3) aerobic bacterial cultures of the small intestine and other organs (the o serogroup and the enterotoxigenicity of the e. coli isolated was determined), 4) de ... | 1976 | 793692 |
| pathogenesis and treatment of escherichia coli infections in calves. | two clearly defined types of e. coli infection are recognised and the factors predisposing and giving rise to pathogenicity are discussed. the mode of action of enterotoxins in the secretary mechanism is thought to be through stimulation of adenyl cyclase activity. treatment and prevention of the disease is considered in relation to the pathogenesis of the infection. | 1976 | 792447 |
| control of rna synthesis by chromatin proteins. | the effect of chromatin proteins on template activity has been studied. using both e. coli rna polymerase and calf thymmus polymerase b we have measured the number of initiation sites on chromatin and various histone-dna complexes. chromatin can be reconstituted with histone proteins alone and this complex is still a restricted template for rna synthesis. the removal of histone f1 causes a large increase in the template activity. chromatin is then treated with micrococcal nuclease and the dna fr ... | 1976 | 787926 |
| [artificial intranasal bacterial invasion in the newborn calf. 2. artificial bacterial invasion using gram-positive and gram-negative strains]. | a micrococcal and a diplocaccal strain isolated from the nasal space of a clinically intact nursed calf were used for artificial bacterial invasion in the first phase of the experiment. application of bacterial suspension prepared from those strains had no effect upon the rise of coli counts in the nasal secretion of nursed calves during their first days of age nor upon the morbidity or mortality of all 677 test animals in comparison to 665 controls. therefore, an avirulent e.-coli strain was us ... | 1976 | 786201 |
| [artificial intranasal bacterial invasion in the newborn calf. 1. quantitative assessment of nasal secretion for certain groups of bacteria]. | a technique has been developed for quantitative assessment of nasal secretion for the presence of specified groups of bacteria, as a contribution to microbiological treatment of the problem of artificial intranasal bacterial invasion in newborn and nursed calf. some 10(6) to 10(7) bacteria were recorded from each gram of nasal secretion in calves exposed to natural bacterial invasion. micrococci and green diplococci were very rare, with only 10(3) to 10(4) or hardly up to 10(5) bacteria per gram ... | 1976 | 786200 |
| etiologic diagnosis of diarrheal disease of calves: frequency and methods for detecting enterotoxin and k99 antigen production by escherichia cola. | escherichia coli isolated from calves in minnesota and montana were tested for enterotoxigenicity via bio-assay of cell-free broth culture fluid and for k99 antigen via a serum agglutination test. infant mice were used to assay for heat-stable enterotoxin (st), and adrenal cells in culture were used to assay for heat-labile enterotoxin (lt). forty-six of the 345 e coli isolates produced st enterotoxin, but none produced lt enterotoxin. thirty-five of the 46 enterotoxigenic isolates had k99 antig ... | 1976 | 786085 |
| vaccination of cows with an escherichia coli bacterin for the prevention of naturally occurring diarrheal disease in their calves. | a formalin-killed escherichia coli bacterin composed of 6 enterotoxigenic strains of the organism prepared from calves with diarrheal disease with field tested for efficacy against naturally occurring diarrheal disease in young calves. the bacterin was tested in 23 privately owned beef herds in montana involving 3,508 cows and their calves. about half the cows in each herd were given 2 subcutaneous vaccinations before calving and the other half (controls) were injected twice with a placebo. almo ... | 1976 | 779544 |
| conjugated polyene fatty acids as fluorescent membrane probes: model system studies. | the use of conjugated polyene fatty acids as probes of membrane structure is examined, alpha- and beta-parinaric acid (cis, trans, trans, cis- and all trans-9, 11, 13, 15-octadecatetraenoic acid) and synthetic lecithins containing an alpha-parinaric acid chai in position 2 have been prepared, and their absorption and fluorescence properties have been determined. their absorption spectra are at sufficiently long wavelength to be unobscured by cellular chromophores such as nucleotides and aromatic ... | 1976 | 778493 |
| effect of coliform challenge at milking time on new udder infections. | this project was designed to study rates of infection in udders of cows exposed to an escherichia coli broth culture at milking time. forty holstein cows of varied stages of lactation were divided randomly into three treatment and one control group of ten cows each. the treatment groups were exposed for 3 wk to an escherichia coli broth of 10(9) colony forming units per ml at milking time by either 1) dipping teat ends in broth before milking, 2) spraying the udder and leaving it dripping wet du ... | 1976 | 777057 |
| occurrence and characteristics of enterotoxigenic escherichia coli isolated from calves with diarrhea. | of 1,004 isolates of escherichia coli obtained during the spring of 1975 in seven different states from calves with diarrhea, 124 isolates were enterotoxigenic based upon ability to cause distention of the calf ligated intestinal segment. isolates of enterotoxigenic e. coli (etec) were obtained from calves in six of the seven states. etec were detected in calves in 118 of 355 herds in montana during the 1974 and 1975 spring beef calving seasons. the occurrence and serotypes of etec isolated from ... | 1976 | 776828 |
| cytidine diphosphate diglyceride of bovine brain. positional distribution of fatty acids and analysis of major molecular species. | a method is described for the isolation of cdp-diglyceride from bovine brain. yields of the product ranged from 9.2-15.5 mumol per kilogram of tissue, which corresponds to about 1% of the level of phosphatidic acid. mild alkaline hydrolysis of the product gave three water-soluble phosphate esters which had the same electrophoretic mobilities as cmp, cdp-glycerol and glycerol 3-phosphate. the liponucleotide was quantitatively hydrolysed by cdp-diglyceride hydrolase from escherichia coli to phosph ... | 1976 | 776622 |
| clinical pharmacology of oxolinic acid in young dairy calves. | the in vitro sensitivity to oxolinic acid shown by pathogenic gram-negative bacterial isolates from young calves with diarrhea, pneumonia, and septicemia was investigated by the bute dilution method. minimal bactericidal concentrations of the drug for 65.5% of the isolates were less than or equal to 1.56 mug/ml and for 90%, less than or equal to 6.25 mug/ml. cross resistance between oxolinic acid and chloramphenicol, streptomycin, neomycin, colistin, ampicillin, gentamicin, and oxytetracycline w ... | 1976 | 776048 |
| antibodies to the triplet codons aaa, aac, and aug: reactions with nucleic acids. | antibodies specific for the trinucleotide codons aaa, aug, and aac were examined for their reactions with nucleic acids. anti-aug and anti-aac precipitated denatured dna. anti-aaa did not, and moreover, the binding of a tritiated aaa derivative to anti-aaa was not inhibited by denatured dna. radioligand-binding studies showed that anti-aaa was highly specific for the triplet sequence, some cross-reactions occurring with di-a and tetra-a but little with a and poly(a). the anti-codons did not prec ... | 1976 | 774989 |
| [the effect of dna isolated from different sources and different physico-chemical conditions on candida tropicalis strain d-2 his-]. | mutagenic effect of dna, isolated from escherichia coli, actinomyces olivaceus and calf thymus, on hystidine-deficient strain of candida tropicalis d-2 is demonstrated. | 1975 | 773761 |
| endotoxin enhancement of plasma cell tumor development in mice given injections of mineral oil. | bacterial endotoxins administered to balb/c mice given i.p. mineral oil cause an increased incidence of plasma cell tumors, compared with mice given either oil or antigens alone, or oil plus antigen other than endotoxin. endotoxin in ng doses was more effective than in mug doses. | 1976 | 773532 |
| local immune responses in the bovine fetus vaccinated in utero with escherichia coli antigen. | using specific immunofluorescent examinations, the local immune responses were studied in 14 calves prenatally vaccinated (10 to 50 days before birth) with escherichia coli (o26:k60:nm) antigen or sterile saline solution. all calves were colostrum-deprived, were given oral doses of homologous organisms (killed or live), and were necropsied either at birth or within 12 days after birth. immunofluorescent plasma cells were not seen in duodenum, jejunum, jejunal lymph nodes, ileum, ileal lymph node ... | 1976 | 769610 |
| [transcription of dna by rna polymerases of e. coli and calf thymus]. | the paper deals with the comparative investigation of initiation and in vitro rna synthesis on dna template by e. coli rna polymerase and b-form of calf thymus rna polymerase. it was shown in hybridization experiments that in the range of cot values between 10(2) and 10(4) rna synthesized by calf thymus rna polymerase was hybridized with homologous dna more effectively than rna synthesized by e. coli rna polymerase. no differences were observed in the case of low cot values. rna chains synthesiz ... | 1975 | 768744 |
| [residues of antimicrobial materials in the effluents of livestock farms (author's transl)]. | forty water samples were taken from streams of three different regions of belgium, with intensive husbandry and examined for residual microbiological activity. in some samples, activity was detected against staphylococcus aureus; none of them were active against e. coli and c. albicans. the substances in the few positive samples were not identified. with this low activity and the small proportion of positive samples (12 out of 41) any influence on the water biosphere due to the methods of farmin ... | 1976 | 766304 |
| passive oral immunization with bovine immunoglobulins: enterpathogenic escherichia coli from infants and bovine anti-e. coli lactoserum assayed in the rabbit ileal loop model. | the effect of immune bovine lactoserum (bls) antipolyvalent enteropathogenic escherichia coli on bacterial growth, viability and bacteria-induced fluid accumulation was examined in rabbit ileal loops. human enteropathogenic e. coli strains 0125:k70 (b15), 0111:k58 (b4) and 055:k59 (b5) (1-3 x 10(9) per inoculum) induced secretion of 4-6 ml fluid per 10 cm loop. this effect was inhibited effectively by bls (corresponding to 50 mg igg 1 per loop) while the viability of bacteria counts decreased 2- ... | 1975 | 765710 |
| experimental neonatal colibacillosis in cows: serological studies. | serological studies of passive immunity in experimentally induced bovine colibacillosis was studied in a 41-cow university herd. pregnant dams were antigenized prepartum with two injections administered by the subcutaneous and intrammamary routes with one of four vaccine preparations (killed bacteria, live bacteria, culture supernatant, or heart infusion broth [control]). the data indicate that 77% of the neonates born to vaccinated dams were strongly protected against oral challenge with escher ... | 1976 | 765285 |
| [decomposition of steroids during incubation with beta-glucuronidase and during storage of urine]. | androsterone, etiocholanolone, pregnanetriol, dehydroepiandrosterone, pregnanediol, tetrahydrocortisol, 5-pregnenolone and 11-beta-oh-androsterone were incubated with beta-glucuronidase preparations (helix pomatia, bovine liver and e. coli) for 96 hrs at 37 degrees c. after extraction and silylation they were gas-chromatographed. the first 3 steroids were left practically intact. the least decomposition of the last 5 steroids occurred with the liver enzyme. testosterone and 11-ketoandrosterone w ... | 1975 | 765125 |
| inactivation by detergents of the proline transport system in membrane vesicles from escherichia coli and its reactivation by bovine serum albumin. | the proline transport system of membrane vesicles from escherichia coli was inactivated by a low concentration of detergents such as deoxycholate, dodecyl sulfate and triton x-100. the addition of a large amount of bovine serum albumin to membrane vesicles which had been treated with one of these detergents resulted in the restoration of the proline transport activity. the restoration of the transport activity by bovine serum albumain was most remarkable with the deoxycholate-inactivated membran ... | 1976 | 764873 |
| anomalous reaction of 4-chloro-7-nitrobenzofurazan with thiol compounds. | the kinetics of reaction of 4-chloro-7-nitrobenzofurazan with thiol groups at ph values above 5 cannot be accounted for solely on the basis of formation of a single product, the 4-thio derivative. spectroscopic observations indicate that, in addition to the 4-thio derivative, at least two other products are formed. one of these, referred to as p1, is most likely a reversible complex of thiol compound and 4-chloro-7-nitrobenzofurazan of the meisenheimer type. the other product, p2, which forms pr ... | 1979 | 435240 |
| diethylaminoalkyloxycoumarin and -furocoumarin derivatives. | four series of diethylaminoalkoxyderivatives of coumarin (at the positions 4 and 7) and furocoumarin (at the positions 5 and 8) have been prepared, in order to study their complexing capacity with dna, these compounds having a double possibility of interaction, that is intercalation of the aromatic moiety between two base pairs and formation of an electrostatic bond between their terminal amino group and a phosphoric group of dna. some biological effects connected with the interaction with dna h ... | 1979 | 399924 |
| [immunobiological activity of various blood serum fractions from cattle and swine. the dynamics of ig in specific serum against colibacillosis in pigs]. | the level of the igm and igg agglutinating antibody specific hyperimmune serum against coli-bacteriosis and its fractions were studied. the serum was obtained at various stages of the preparation, hyperimmunization and exploitation of serum producing swine by using the 2-mercapto-ethanol test. it was established that serum of the total globulin, alpha-beta-globulin and gamma-globulin fractions contains igm and igg agglutinating antibodies against e. coli. these immunoglobulin classes are synthet ... | 1979 | 396718 |
| vaccination of the dam by the intramuscular or deep subcutaneous route to prevent neonatal calf enteric colibacillosis. | comparison of colostrum-induced immunities in calves was made by challenge exposure with escherichia coli. these calves were delivered of cows which were vaccinated intramuscularly or deep subcutaneously (in the region of the mammary lymph nodes) with strain b44 e coli bacterin during the last trimester of pregnancy. the calf of each cow was allowed to nurse colostrum naturally after birth. cows vaccinated by either route of administration were capable of providing increased resistance to their ... | 1979 | 393149 |
| the pathogenesis of enteric colibacillosis in neonatal unsuckled calves. | the development of pathological lesions in the small intestine of neonatal calves is described. seven newborn calves were challenged orally with a known enteropathogenic strain of e coli 0101k?(a) and killed at varying times after inoculation. adhesion of bacteria to the mucosa of the small intestine was observed in all calves. a few organisms were seen in the distal small intestine at three hours after inoculation and thereafter adhesion progressed anteriorly along the intestine in calves kille ... | 1979 | 390852 |
| recurrent coliform mastitis in the dairy cow. | daily monitoring of milk over a 120-day period for bacteria and neutrophil counts revealed that following experimental e coli mastitis, five out of 28 infections resulted in the development of a long continued and recurring condition. intermittent periods of acute inflammation were observed in the gland, pyrexia was noted, and if bacteria were isolated they were always of the same serotype as the original infecting strain. failure to isolate bacteria and the lack of overt inflammation during per ... | 1979 | 390847 |
| the effect of an intramammary infusion of endotoxin on experimentally induced mycoplasmal mastitis. | the infusion of 10 microgram of endotoxin lipopolysaccharide from escherichia coli into the mammary gland of four cows 16 h before inoculation with ureaplasmas did not prevent, or even diminish, the subsequent ureaplasmal mastitis. there was no reduction in the severity or duration of the inflammatory cell response in milk or in the clinical appearance of the resulting mastitis. also, the excretion of ureaplasmas was not reduced. a similar experiment with mycoplasma dispar in two cows demonstrat ... | 1979 | 390046 |
| a rhelogical separator for very large dna molecules. | we present a rheological separation method for dna molecules in which their deformability is used to advantage. this is the "radial migration method"; here we present experimental verification of the principle, theory having been reported elsewhere. the main conclusions are: (1) the theory is reasonably good; (2) radial migration is highly sensitive to the molecular weight, as predicted, and (3) intact t2 dna (1.25 x 108 daltons) can be made to migrate about three centimeters in less than three ... | 1979 | 388346 |
| ribosome structure: localization of 3' end of rna in small subunit by immunoelectronmicroscopy. | the 3' end of the rna in the 30s ribosomal subunit of escherichia coli has been modified by oxidation with sodium periodate and conjugation with the (mono) dinitrophenyl derivative of ethylenediamine. antibodies, induced with dinitrophenyl-bovine serum albumin, interact with the modified ribosomal subunits. electron micrographs of negatively stained antibody-subunit complexes show individual ribosomal subunits to which a single antibody molecule is bound and subunit dimers cross-linked by an igg ... | 1979 | 386348 |
| the origin of yolk-dna in xenopus laevis. | xenopus laevis serum and plasma was found to contain an average of 25 microgram dna/ml. isolated x. laevis oocytes incubated in medium containing 25 microgram dna/ml labeled with either 125i, 32p or 14c and from three different sources (bovine, e. coli and x. laevis), incorporated the label at an average rate of 0.11 ng.mm-2.hr-1. sucrose gradient fractionation of oocytes revealed that 40-75% of the acid-precipitable label incorporated was associated with the yolk platelets. additional incubatio ... | 1979 | 385804 |
| circular dichroism anisotrophy of dna with different modifications at n7 of guanine. | the complexex dna-ag1+, dna-cu1+, protonated dna and dna methylated at n7 of guanine were oriented by pumping the solutions through a multicapillary cell in the direction of a light beam. the cd components along the dna axis, delta epsilon parallel, and normal to it, 2 delta epsilon perpendicular, were calculated from the cd spectra of the oriented samples by the method of chung and holzwarth, (1975) j. mol. biol. 92, 449--466. it was shown that in most cases, except that of the protonated dna, ... | 1979 | 385054 |
| the pathogenesis of experimental escherichia coli mastitis in newly calved dairy cows. | experimental infections of the mammary gland of newly calved cows with 500 serum resistant escherichia coli produced a very severe form of mastitis when compared with animals in mid-lactation. ten hours after infection the bacteria had multiplied in the milk to very high numbers (10(6)--10(7)/ml) and the animals showed signs of pyrexia, anorexia and diarrhoea. initially the gland and milk showed little or no clinical signs of mastitis, but later the secretion became a viscous, serous fluid with ... | 1979 | 382318 |
| the survival of serum resistant escherichia coli in the bovine mammary gland following experimental infection. | serum resistant strains of escherichia coli were injected into one or two quarters of the udders of eight healthy dairy cows. animals receiving infection into two quarters showed variation in their ability to eliminate the bacteria. this variation extended from elimination from both glands to complete failure to remove the organisms from either gland. in most cases, the organisms were removed from one gland before the clinical signs of infection were observed, but persisted in the other gland fo ... | 1979 | 382316 |
| [interaction of oligophosphates of pyridoxal with certain enzymes of polynucleotide synthesis]. | the interaction of pyridoxal, pyridoxal-5'-mono-, di- and triphosphate with certain enzymes of polynucleotide synthesis (dna-dependent rna polymerase, dna-dependent dna polymerase i and polynucleotide phosphorylase from escherichia coli and terminal deoxyribonucleotide transferase from calf thymus) was studied. all compounds tested was found to be reversible and competitive inhibitors of these enzymes. the reduction of the enzyme-inhibitor complex with nabh4 gives rise to the complete irreversib ... | 1979 | 381898 |
| early mitochondrial damage in the induction of haemorrhagic necrosis in the crocker sarcoma (s 180) by endotoxin. | disturbances in the functional properties of tumor mitochondria have been studied during the course of induction of haemorrhage brought about by endotoxin in the murine crocker sarcoma (s 180). extensive impairment of function was already present in mitochondria isolated from control tumors, as shown by low respiratory control ratios. the existing mitochondrial damage intensified promptly in response to injection of endotoxin long before the onset of haemorrhage at 4 h. the nature of the additio ... | 1979 | 381316 |
| leucocytic changes in cows given intravenous injections of e coli endotoxin. | 1979 | 380122 |