Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| characterization of a laboratory-generated variant of bps beta-lactamase from burkholderia pseudomallei that hydrolyses ceftazidime. | burkholderia pseudomallei produces an ambler class a beta-lactamase, known as bps-1. the beta-lactamase gene from a laboratory-derived, ceftazidime-resistant strain of b. pseudomallei (lh-1-2) was cloned and expressed in escherichia coli. the beta-lactamase, named bps-1m, had an identical isoelectric focusing point (pi 7.7) to that of bps-1, but differed in having a stronger hydrolytic activity against ceftazidime. susceptibility testing showed that bps-1m when expressed in e. coli conferred res ... | 2002 | 12407130 |
| genetic analysis of functions involved in the late stages of biofilm development in burkholderia cepacia h111. | burkholderia cepacia and pseudomonas aeruginosa often co-exist as mixed biofilms in the lungs of patients suffering from cystic fibrosis (cf). here, we report the isolation of 13 random mini-tn5 insertion mutants of b. cepacia h111 that are defective in biofilm formation on a polystyrene surface. we show that the screening procedure used in this study is biased towards mutants defective in the late stages of biofilm development. a detailed quantitative analysis of the biofilm structures formed b ... | 2002 | 12406218 |
| increased treatment requirements of patients with cystic fibrosis who harbour a highly transmissible strain of pseudomonas aeruginosa. | a group of patients who harbour the same highly transmissible strain of pseudomonas aeruginosa were identified at a cystic fibrosis (cf) centre. isolates of this strain display a number of unusual phenotypic features including resistance to most typical antipseudomonal antibiotics. a study was undertaken to see if there was a difference in treatment requirements between cf patients with chronic infection with their own unique p aeruginosa strains (group 1) and those who harbour a highly transmis ... | 2002 | 12403871 |
| physiological and cellular responses of the 2,4-d degrading bacterium, burkholderia cepacia yk-2, to the phenoxyherbicides 2,4-d and 2,4,5-t. | our previous research has demonstrated that novel 43-kda dnak and 41-kda groel proteins are synthesized in burkholderia sp. yk-2 in response to sublethal concentrations of 2,4-d stress [cho et al. (2000) curr microbiol 41:33-38]. in this study, we have extended this work to examine the cellular responses of strain yk-2 to stresses induced in response to the phenoxyherbicides 2,4-d or 2,4,5-t. strain yk-2 exhibited a more sensitive response to 2,4,5-t stress than to 2,4-d stress, as shown in phys ... | 2002 | 12402082 |
| isolation of bacterial strains that produce the endocrine disruptor, octylphenol diethoxylates, in paddy fields. | topsoil samples were collected from 36 different paddy fields in west japan. each soil sample was incubated with a basal salt-medium containing 0.2% oppeo. twelve samples possessed oppeo-degrading activity, from which twelve cultures of oppeo-degrading bacteria were isolated. the isolated bacteria grew on a medium containing 0.2% oppeo as the sole carbon source, and op2eo and op3eo were accumulated in the medium under aerobic conditions. op1eo and octylphenol, which have often been identified in ... | 2002 | 12400675 |
| cloning and characterization of the burkholderia vietnamiensis norm gene encoding a multi-drug efflux protein. | polymyxin b-sensitive mutants in burkholderia vietnamiensis (burkholderia cepacia genomovar v) were generated with a mini-tn5 encoding tetracycline resistance. one of the transposon mutants had an insertion in the norm gene encoding a multi-drug efflux protein. expression of b. vietnamiensis norm in an escherichia coli acrab deletion mutant complemented its norfloxacin hypersensitivity, indicating that the protein functions in drug efflux. however, no effect on antibiotic sensitivity other than ... | 2002 | 12399047 |
| antibiotic resistance in soil and water environments. | seven locations were screened for antibiotic-resistant bacteria using a modified agar dilution technique. isolates resistant to high levels of antibiotics were screened for r plasmids. low-level resistance (25 micro g x ml(-1)) was widespread for ampicillin, penicillin, tetracycline, vancomycin and streptomycin but not for kanamycin. resistant populations dropped sharply at high antibiotic levels, suggesting that intrinsic non-emergent mechanisms were responsible for the multiple drug resistance ... | 2002 | 12396530 |
| tetraponera ants have gut symbionts related to nitrogen-fixing root-nodule bacteria. | some tetraponera ants (formicidae, pseudomyrmecinae) subsist almost entirely on amino acid deficient honeydew secretions of pseudococcids and harbour a dense aggregation of bacterial symbionts in a unique pouch-shaped organ at the junction of the midgut and the intestine. the organ is surrounded by a network of intruding tracheae and malpighian tubules, suggesting that these bacteria are involved in the oxidative recycling of nitrogen-rich metabolic waste. we have examined the ultrastructure of ... | 2002 | 12396501 |
| selection of patients with cystic fibrosis for lung transplantation. | lung transplantation is the most aggressive therapy available for end-stage lung disease from cystic fibrosis (cf). a new predictive survival model of cf uses demographic, fev1, nutritional, microbiologic, and acute exacerbation data to produce precise estimates of 5-year survival. the model improves the ability to select patients most likely to have survival benefit from transplantation. we discuss potential application of the survival model to four distinct groups of patients with cf: (1) cand ... | 2002 | 12394163 |
| the burkholderia pseudomallei oxyr gene: expression analysis and mutant characterization. | burkholderia pseudomallei (bp) is the causative agent of the life-threatening melioidosis in humans. the global transcription factor oxyr gene was isolated and characterized. it is located between recg, encoding a putative dna helicase, and katg, encoding a putative catalase-peroxidase. oxyr is expressed as a monocistronic 1 kb mrna and is induced by oxidative stress compounds. northern, primer extension, and transcription reporter fusion analyses showed that oxyr mrna is induced by 0.2 mm menad ... | 2002 | 12383513 |
| interspecies communication between burkholderia cepacia and pseudomonas aeruginosa. | burkholderia cepacia and pseudomonas aeruginosa are opportunistic pathogens that commonly cause pulmonary infections in cystic fibrosis patients and occasionally co-infect patients' lungs. both organisms possess quorum-sensing systems dependent on n-acyl homoserine lactone (n-acyl-hsl). cross-feeding assays demonstrated that p. aeruginosa and b. cepacia were able to utilize heterologous n-acyl-hsl signaling molecules. the ability of quorum-sensing genes from one species to complement the respect ... | 2002 | 12381027 |
| risk factors for burkholderia cepacia complex colonization and infection among patients with cystic fibrosis. | to determine risk factors for acquiring burkholderia cepacia complex among patients with cystic fibrosis (cf). | 2002 | 12378190 |
| preventing burkholderia cepacia complex infection in cystic fibrosis: is there a middle ground? | 2002 | 12378182 | |
| case 1: assessment. burkholderia cepacia. | 2002 | 12376068 | |
| advances in burkholderia cepacia complex. | burkholderia cepacia is an important opportunistic pathogen in certain compromised hosts, particularly those with either cystic fibrosis (cf) or chronic granulomatous disease. the "family" of bacteria known as b. cepacia is highly heterogeneous and is composed of at least nine discrete species or genomovars, constituting the b. cepacia complex. bacteria from the b. cepacia complex are particularly virulent in susceptible hosts, often causing necrotising invasive infection and death. whereas the ... | 2002 | 12376059 |
| effect of ph on cadmium toxicity, speciation, and accumulation during naphthalene biodegradation. | lowering ph of a microbiological medium from 7 to 4 decreased cadmium toxicity during naphthalene biodegradation by a burkholderia sp. cadmium speciation and cadmium accumulation in the system were studied to explain this effect. cadmium speciation was determined by direct measurement and by geochemical modeling. previous studies have implicated the monovalent hydroxylated cadmium (cdoh+) species in the effect of ph on cadmium toxicity. modeling analysis predicted cdoh+ formation only at very lo ... | 2002 | 12371483 |
| effect of high-temperature short-time (htst) laboratory pasteurization on the survival of burkholderia cepacia complex organisms in whole, low fat and skimmed milks. | 2002 | 12369418 | |
| multiple substrate growth kinetics of leptothrix discophora sp-6. | the growth parameters of leptothrix discophora sp-6 were quantified on the basis of the steady-state concentrations and utilization rates of pyruvate, dissolved oxygen, and concentration of microorganisms in a chemostat operated at 25 degrees c, ph 7.2, and an agitation rate of 350 rpm. the results showed that the microbial growth was limited by both pyruvate and dissolved oxygen. a combined growth kinetics model using monod growth kinetics for pyruvate and tessier growth kinetics for oxygen sho ... | 2002 | 12363350 |
| detecting bacterial colonization of implanted orthopaedic devices by ultrasonication. | glycocalyx-producing bacteria have been observed on orthopaedic devices that were removed for reasons other than infection. it has been suggested that the bacteria adhere to foreign surfaces within a biofilm and elude standard culture techniques. the authors adapted previously used ultrasonication protocols that disrupt the surface biofilm before culturing removed orthopaedic devices from patients without clinical evidence of infection. patients having revision total joint arthroplasty of the hi ... | 2002 | 12360004 |
| sulphoacetaldehyde acetyltransferase yields acetyl phosphate: purification from alcaligenes defragrans and gene clusters in taurine degradation. | the facultatively anaerobic bacterium alcaligenes defragrans nkntau was found to oxidize taurine (2-aminoethanesulphonate) with nitrate as the terminal electron acceptor. taurine was transaminated to 2-sulphoacetaldehyde. this was not converted into sulphite and acetate by a "sulphoacetaldehyde sulpho-lyase" (ec 4.4.1.12), but into sulphite and acetyl phosphate, which was identified by three methods. the enzyme, which required the addition of phosphate, thiamin diphosphate and mg(2+) ions for ac ... | 2003 | 12358600 |
| cloning of the class d beta-lactamase gene from burkholderia pseudomallei and studies on its expression in ceftazidime-susceptible and -resistant strains. | ceftazidime is the antibiotic of choice for the treatment of melioidosis. ceftazidime-resistant burkholderia pseudomallei have been identified and beta-lactamase production implicated in resistance. in this study, 25 strains of b. pseudomallei (15 clinical and 10 environmental strains) were examined for their ability to yield mutants that overexpress beta-lactamase. ceftazidime-resistant mutants were selected readily at high frequency and displayed four- to eight-fold increases in the mics of ce ... | 2002 | 12356787 |
| use of 16s rrna gene sequencing for identification of nonfermenting gram-negative bacilli recovered from patients attending a single cystic fibrosis center. | during 1999, we used partial 16s rrna gene sequencing for the prospective identification of atypical nonfermenting gram-negative bacilli isolated from patients attending our cystic fibrosis center. of 1,093 isolates of nonfermenting gram-negative bacilli recovered from 148 patients, 46 (4.2%) gave problematic results with conventional phenotypic tests. these 46 isolates were genotypically identified as pseudomonas aeruginosa (19 isolates, 12 patients), achromobacter xylosoxidans (10 isolates, 8 ... | 2002 | 12354883 |
| factors governing the activity of lyophilised and immobilised lipase preparations in organic solvents. | active site titration and activity measurements were performed in hexane on lyophilised lipase preparations containing different amounts of phosphate buffer and lipase immobilised on porous polypropylene. lyophilisation of thermomyces lanuginosus lipase with large quantities of phosphate salts (200 mm) increased the specific activity fourfold, and the number of rapidly titratable active sites increased to 50 % from the 13 % observed when smaller amounts of phosphate buffer were used (20 mm) duri ... | 2002 | 12325013 |
| long term results of lung transplantation for cystic fibrosis. | we reviewed our experience with lung transplant for cystic fibrosis (cf) over a 10-year period to identify factors influencing long-term survival. | 2002 | 12297180 |
| differential expression of two catechol 1,2-dioxygenases in burkholderia sp. strain th2. | burkholderia sp. strain th2, a 2-chlorobenzoate (2cb)-degrading bacterium, metabolizes benzoate (ba) and 2cb via catechol. two different gene clusters for the catechol ortho-cleavage pathway (cat1 and cat2) were cloned from th2 and analyzed. sodium dodecyl sulfate-polyacrylamide gel electrophoresis and western blot analysis showed that while both catechol dioxygenases (cata1 and cata2) were produced in ba-grown cells, cata1 was undetectable when strain th2 was grown on 2cb or cis,cis-muconate (c ... | 2002 | 12270830 |
| biofilm formation and acyl homoserine lactone production in the burkholderia cepacia complex. | acyl homoserine lactone (acyl-hsl)-mediated gene regulation has been shown to influence biofilm formation in one burkholderia cepacia cystic fibrosis isolate, but it is not known whether this relationship is a consistent feature of the several genomic species that make up the b. cepacia complex (bcc). we screened strains belonging to genomovars i to v of the bcc for biofilm formation on an abiotic surface and for acyl-hsl synthesis. we determined that organisms from each of these genomovars were ... | 2002 | 12270826 |
| clinical features of community-acquired pneumonia treated at srinagarind hospital, khon kaen, thailand. | pneumonia is a serious illness associated with significant morbidity and mortality. the interpretation guidelines for pneumonia management requires knowledge of both the clinical presentation of the disease and local epidemiology. we studied the clinical features, initial laboratory results, antibiotic sensitivities, and outcomes of patients diagnosed with acute community-acquired pneumonia between january 1999 and december 2000 at srinagarind hospital. the causative organisms were identified in ... | 2002 | 12236437 |
| biochemical-genetic analysis and distribution of des-1, an ambler class a extended-spectrum beta-lactamase from desulfovibrio desulfuricans. | desulfovibrio spp. are gram-negative anaerobes phylogenetically related to bacteroides spp., which are rarely isolated and which are mostly isolated from intra-abdominal abscesses. desulfovibrio desulfuricans clinical isolate d3 had a clavulanic acid-inhibited beta-lactam resistance profile and was resistant to some expanded-spectrum cephalosporins. a beta-lactamase gene, bla(des-1), was cloned from whole-cell dna of isolate d3 and expressed in escherichia coli. purified beta-lactamase des-1, wi ... | 2002 | 12234847 |
| diversity of burkholderia isolates from woodland rhizosphere environments. | determination of genetic diversity among uk burkholderia cepacia isolates from various environmental niches, principally woodland tree rhizospheres and onions. | 2002 | 12234345 |
| identification and characterization of a novel murine beta-defensin-related gene. | beta-defensins comprise a family of cationic peptides, which are predominately expressed at epithelial surfaces and have a broad-range antimicrobial activity. we have assembled two bac-based contigs from the chromosomal region 8a4 that contain the murine defensins, and we have mapped six reported beta-defensin genes. in addition, we have isolated and functionally characterized a novel beta-defensin gene that deviates from the canonical six cysteine motif present in the mature functional peptide ... | 2002 | 12226710 |
| short report: a rapid method for the differentiation of burkholderia pseudomallei and burkholderia thailandensis. | a rapid method for the identification and differentiation of burkholderia pseudomallei and burkholderia thailandensis colonies is described. it consists of simultaneous use of 2 monoclonal antibody-based latex agglutination test systems. the anti-lipopolysaccharide test reacts with both species, whereas the anti-exopolysaccharide reacts only with b. pseudomallei. compared with classical biochemical tests, the method is highly reproducible and accurate. it is particularly useful for the identific ... | 2002 | 12224587 |
| bacterial diversity associated with subalpine fir (abies lasiocarpa) ectomycorrhizae following wildfire and salvage-logging in central british columbia. | to assess the effect of fire and salvage logging on the diversity of mycorrhizal-bacterial communities, bacteria associated with cenococcum, thelephora, tomentella, russulaceae, and e-strain ectomycorrhizae (ecm) of abies lasiocarpa seedlings were characterized using two approaches. first, bacteria were isolated and characterized by biolog, gas chromatography fatty acid methyl ester (gc-fame), and amplified 16s rdna restriction analysis (ardra). the bacterial communities retrieved from ecm from ... | 2002 | 12224560 |
| trichromatic concept optimizes mad experiments in synchrotron x-ray crystallography. | the trichromatic concept is a new synchrotron beamline design that optimizes mad experiments by reducing systematic experimental errors with three-colored and coaxial synchrotron x-ray beams produced by a tandem vertical undulator and trichromator. the concept enables rapid and flexible switching of three defined wavelengths, and extends the flexibility of experimental design for mad data collection. thus, we can collect mad data taking into account time series effects such as radiation damage. ... | 2002 | 12220492 |
| use of selective medium for burkholderia cepacia isolation in respiratory samples from cystic fibrosis patients. | burkholderia cepacia colonizes cystic fibrosis (cf) patients. we evaluated the impact of the use of a selective medium in the rate of b. cepacia recovery from respiratory samples of cf patients. during a 6-month period, respiratory samples were collected from 106 cf patients and cultivated on selective media including a b. cepacia selective medium. confirmation of the identity of b. cepacia isolates was carried out by species specific pcr and determination of genomovar status performed by a sequ ... | 2002 | 12219111 |
| burkholderia pseudomallei: abscess in an unusual site. | melioidosis is an infection caused by burkholderia pseudomallei. it is an important human pathogen in tropical area. the clinical manifestations are protean and multisystem involvement. we report an unusual case of melioidosis with abscess at root of mesentery in an elderly, non-insulin dependent diabetic thai women. she presented with prolonged fever and chronic abdominal pain. the early clinical diagnosis was carcinomatous mass with peritonitis. diagnosis of melioidosis arose from the surgical ... | 2002 | 12215696 |
| complete characterisation of tn5530 from burkholderia cepacia strain 2a (pijb1) and studies of 2,4-dichlorophenoxyacetate uptake by the organism. | the complete genetic characterisation of tn5530 in burkholderia cepacia strain 2a (pijb1) has been accomplished, indicating that it is a tn3-like transposon with a complex structure bearing operons for the catabolism of 2,4-dichlorophenoxyacetate (2,4-d) and malonate. tn5530 is terminated at both ends by the is1071::is1471 element and the 2,4-d- and malonate-dissimilatory operons are separated by a region encoding a puta and lrp gene and a gene encoding a chloride channel protein. the chloride c ... | 2002 | 12206751 |
| evaluation of restriction fragment length polymorphism analysis of 16s rdna as a tool for genomovar characterisation within the burkholderia cepacia complex. | a total of 154 burkholderia cepacia complex strains, isolated from cystic fibrosis and non-cystic fibrosis patients and the environment, representing all nine genomovars and a putative tenth, were analysed by 16s rdna-restriction fragment length polymorphism using the restriction enzymes alui, cfoi and ddei. examining this diverse strain collection resulted in very diverse restriction patterns. only b. cepacia genomovar vi could be identified unambiguously. the same restriction patterns were obs ... | 2002 | 12204364 |
| direct pcr detection of burkholderia cepacia complex and identification of its genomovars by using sputum as source of dna. | we developed a nested pcr assay that detects the reca gene of the burkholderia cepacia complex in sputum. the product of the first pcr round is also used to identify the genomovar of the pathogen. the protocol achieves high sensitivity and specificity with simple interpretation of genomovar status. | 2002 | 12202599 |
| comparative assessment of genotyping methods for epidemiologic study of burkholderia cepacia genomovar iii. | we analyzed a collection of 97 well-characterized burkholderia cepacia genomovar iii isolates to evaluate multiple genomic typing systems, including pulsed-field gel electrophoresis (pfge), box-pcr fingerprinting and random amplified polymorphic dna (rapd) typing. the typeability, reproducibility, and discriminatory power of these techniques were evaluated, and the results were compared to each other and to data obtained in previous studies by using multilocus restriction typing (mlrt). all meth ... | 2002 | 12202570 |
| comparison of rapid, automated ribotyping and dna macrorestriction analysis of burkholderia pseudomallei. | an automated ribotyping device (riboprinter) was used to determine the ribotypes of a collection of burkholderia pseudomallei isolates. in a preliminary evaluation with the restriction enzymes bamhi and ecori, the protocol with ecori was more discriminating. the reproducibilities of the ribotypes obtained with ecori (ecori ribotypes) were determined by testing three levels of bacterial loads. the performance of the manufacturer's software was assessed by comparing the machine-optimized ribotypes ... | 2002 | 12202553 |
| psychological consequences of segregation resulting from chronic burkholderia cepacia infection in adults with cf. | 2002 | 12200517 | |
| prba, a gene coding for an esterase hydrolyzing parabens in enterobacter cloacae and enterobacter gergoviae strains. | the new gene prba encodes an esterase responsible for the hydrolysis of the ester bond of parabens in enterobacter cloacae strain em. this gene is located on the chromosome of strain em and was cloned by several pcr approaches. the prba gene codes for an immature protein of 533 amino acids, the first 31 of which represent a proposed signal peptide yielding a mature protein of a putative molecular mass of 54.6 kda. this enzyme presents analogies with other type b carboxylesterases, mainly of euka ... | 2002 | 12193616 |
| enhanced phosphate uptake and polyphosphate accumulation in burkholderia cepacia grown under low ph conditions. | of bacterial cells in a sample of activated sludge, 34% contained detectable intracellular polyphosphate inclusions following neisser staining, when grown on glucose/mineral salts medium at ph 5.5; at ph 7.5 only 7% of cells visibly accumulated polyphosphate. in a sludge isolate of burkholderia cepacia chosen for further study, maximal removal of phosphate and accumulation of polyphosphate occurred at ph 5.5; levels were up to 220% and 330% higher, respectively, than in cells grown at ph 7.5. du ... | 2002 | 12187377 |
| phage display of recombinant antibodies toward burkholderia pseudomallei exotoxin. | we have used the phagemid pcomb3h to construct recombinant phages displaying the single chain variable fragment (scfv) towards exotoxin of burkholderia pseudomallei. variable heavy and light chain fragments were amplified from the hybridoma 6e6a8f3b line, with a wide spectrum of primers specific to mouse antibody genes. through overlapping extension polymerase chain reaction, the heavy and light chain fragments were linked to form the scfv which was subsequently cloned into the phage display vec ... | 2002 | 12186782 |
| a mutant of burkholderia pseudomallei, auxotrophic in the branched chain amino acid biosynthetic pathway, is attenuated and protective in a murine model of melioidosis. | using a transposon mutagenesis approach, we have identified a mutant of burkholderia pseudomallei that is auxotrophic for branched chain amino acids. the transposon was shown to have interrupted the ilvi gene encoding the large subunit of the acetolactate synthase enzyme. compared to the wild type, this mutant was significantly attenuated in a murine model of disease. mice inoculated intraperitoneally with the auxotrophic mutant, 35 days prior to challenge, were protected against a challenge dos ... | 2002 | 12183585 |
| [cloning and expression of 7.55 kb kpni fragment of burkholderia pseudomallei ppm1 plasmid in escherichia coli]. | bamhi, sali, psti, and kpni fragments of ppm1 (b. pseudomallei 12.95 kb plasmid) were cloned in e. coli. the recombinant clones carrying a 7.55 kb kpni fragment of ppm1 were highly resistant to several aminoglycosides (streptomycin, kanamycin, and gentamycin) and fluoroguinolones (perfloxacin, ofloxacin). two outer membrane proteins (23 and 27 kda) absent in e. coli and capable to form 120 kda oligomer complex were detected by the western blot method in the strain carrying recombinant ps19 plasm ... | 2002 | 12180019 |
| the principal determinants for the structure of the substrate-binding pocket are located within a central core of a biphenyl dioxygenase alpha subunit. | protein engineering by segment exchange was used to distinguish between regions of major and minor influence on the structure of the substrate-binding pocket of a biphenyl dioxygenase (bdo). eight chimaeric enzyme systems were generated that each consisted of a hybrid hydroxylase alpha subunit (bpha1) containing segments from burkholderia sp. strain lb400 and rhodococcus globerulus p6, and of a hydroxylase beta subunit (bpha2), a ferredoxin (bpha3) and a ferredoxin reductase (bpha4) from strain ... | 2002 | 12177337 |
| identification and antimicrobial susceptibility testing of clinical isolates of nonfermenting gram-negative bacteria by the phoenix automated microbiology system. | the phoenix automated microbiology system (becton dickinson, sparks, md) was evaluated for its ability to identify nonfermenting gram-negative pathogens and measure their drug susceptibility. isolates producing rare extended-spectrum beta-lactamases (per-1, imp-2, vim-1, and vim-2) were included in the study. species identification was compared to that given by the atb system (bio-mérieux, marcy l'etoile, france), whereas susceptibility results were compared to those produced by a reference brot ... | 2002 | 12173774 |
| peripheral blood lymphocyte subsets in acute human melioidosis. | 2002 | 12172754 | |
| [glanders--a potential disease for biological warfare in humans and animals]. | infection with burkholderia mallei (formerly pseudomonas mallei) can cause a subcutaneous infection known as "farcy" or can disseminate to condition known as glanders. it is primarily a disease affecting horses, donkeys and mules. in humans, glanders can produce four types of disease: localized form, pulmonary form, septicemia, and chronic form. necrosis of the tracheobronchial tree and pustular skin lesions characterize acute infection with b. mallei. other symptoms include febrile pneumonia, i ... | 2002 | 12170562 |
| influence of taxonomic status on the in vitro antimicrobial susceptibility of the burkholderia cepacia complex. | the burkholderia cepacia complex is a diverse group of human pathogens that cause life-threatening lung infections in patients with cystic fibrosis or chronic granulomatous disease, and in patients requiring intensive care. most previous antimicrobial susceptibility studies of these bacteria were performed before recent major revisions in the taxonomy of these bacteria. we determined the in vitro susceptibility of 65 b. cepacia complex isolates from clinical and environmental sources, representi ... | 2002 | 12161410 |
| effect of burkholderia cepacia infection in the clinical course of patients with cystic fibrosis: a pilot study in a sydney clinic. | colonisation with burkholderia cepacia complex in patients with cystic fibrosis (cf) has been associated with adverse outcomes. the aim of the present study was to determine the actuarial survival of cf patients colonized with b. cepacia and to evaluate the efficacy of the royal prince alfred hospital segregation policy. a secondary aim was to characterize the specific genomovars and strains of b. cepacia isolated in an australian clinic. | 2002 | 12153690 |
| [cloning, sequencing and high expression in escherichia coli of d-hydantoinase gene from burkholderia pickettii]. | a strain, mmr003, used for d-p-hpg production in industry was classified as burkholderia pickettii by morphological observation and biochemical characterization. the gene encoding the d-hydantoinase enzyme was cloned, sequenced and expressed in escherichia coli. the nucleotide sequence of the 5.0 kb insert of subclone pxz-total was determined. one open reading frame of 1374 bp was found and predicted to encode a polypeptide consisting of 458 amino acids in size of 50 kd. the amino acid sequence ... | 2002 | 12148274 |
| culture-based and non-growth-dependent detection of the burkholderia cepacia complex in soil environments. | burkholderia cepacia complex (bcc) bacteria reside in soil, plant rhizospheres, and water, but their prevalence and distribution in outdoor environments is not clear. we sampled a variety of soil and rhizosphere environments with which people may have contact: playgrounds, athletic fields, parks, hiking trails, residential yards, and gardens. a total of 91 sites was sampled in three large u.s. cities. in the first phase of the study, putative bcc isolates were recovered on burkholderia cepacia s ... | 2002 | 12147469 |
| a simple method to detect and differentiate burkholderia pseudomallei and burkholderia thailandensis using specific flagellin gene primers. | we have previously shown that burkholderia pseudomallei, the causative pathogen of melioidosis, may be discriminated from the closely related non-pathogenic species burkholderia thailandensis by the presence of a 15 base pair deletion in the flagellin gene of b. thailandensis. using specific flagellin gene primers flanking the distinctive region, pcr products of 191 and 176 bp in size were detected for b. pseudomallei and b. thailandensis, respectively. the sensitivity of detection is 20-80 colo ... | 2002 | 12144773 |
| cutaneous melioidosis. | melioidosis is a rare tropical disease caused by infection with the bacterium burkholderia pseudomallei. it occurs predominantly in south-east asia, northern and central australia and central and south america. patients often present to the internal medicine physicians with a severe, potentially fatal sepsis. we report three patients who presented to our dermatology department with cutaneous melioidosis. | 2002 | 12139670 |
| burkholderia pseudomallei infections. | 2002 | 12139165 | |
| demonstration of a cell-mediated immune response in melioidosis. | melioidosis is a bacterial infection caused by burkholderia pseudomallei. the aim of this study was to determine whether a cell-mediated adaptive immune response against b. pseudomallei developed in patients who had recovered from melioidosis. lymphocyte proliferation assays were done on peripheral blood mononuclear cells from patients (n=13) and control subjects (n=10) to determine the lymphocyte response to b. pseudomallei antigens. production of interferon-gamma and interleukin-10 was also de ... | 2002 | 12134268 |
| characterisation of an acapsular mutant of burkholderia pseudomallei identified by signature tagged mutagenesis. | a burkholderia pseudomallei mutant which was attenuated in a mouse model of melioidosis was identified by a signature tagged mutagenesis approach. the transposon was shown to be inserted into a gene within the capsular biosynthetic operon. compared with the wild-type bacteria this mutant demonstrated a 10(5)-fold increase in the median lethal dose in a mouse model and it did not react with a monoclonal antibody against high mol. wt polysaccharide of b. pseudomallei. to determine the kinetics of ... | 2002 | 12132769 |
| burkholderia cepacia complex infection in patients with cystic fibrosis. | the word 'complex' has several meanings and synonyms such as composite, obsession, heterogeneous, mixed and network, can all be used in its place. our obsession with bacteria from the burkholderia cepacia complex started in the early 1990s. in less than 10 years, we have seen the status of this bacterium move from: (i) a lesser known pseudomonad opportunist pathogen, (ii) to devastating infections transmitted between patients with cystic fibrosis (cf), (iii) through divisions into several new sp ... | 2002 | 12132768 |
| burkholderia gladioli keratitis associated with consecutive recurrent endophthalmitis. | to report a case of burkholderia gladioli keratitis with consecutive endophthalmitis. | 2002 | 12131039 |
| synergistic activity of the novel des-fluoro(6) quinolone, garenoxacin (bms-284756), in combination with other antimicrobial agents against pseudomonas aeruginosa and related species. | non-fermentative gram-negative bacteria (pseudomonas aeruginosa, burkholderia cepacia, stenotrophomonas maltophilia and acinetobacter spp.) are intrinsically less susceptible to many antimicrobial agents. two-drug combinations have been used to treat infections caused by less susceptible pathogens. in this study, the antibacterial activity of garenoxacin (garx) with non-quinolones was examined. the non-quinolones evaluated were cefepime (cepi), imipenem (imip), aztreonam (aztr), piperacillin-taz ... | 2002 | 12127712 |
| primary melioidotic prostatic abscess: presentation, diagnosis and management. | in south-east asia and northern australia, melioidosis (infection with burkholderia pseudomallei) is a known cause of severe community-acquired sepsis. however, melioidosis presenting primarily as prostatic abscesses is very rare. | 2002 | 12121159 |
| patterns of epithelial cell invasion by different species of the burkholderia cepacia complex in well-differentiated human airway epithelia. | burkholderia cepacia has emerged as a serious respiratory pathogen in cystic fibrosis (cf) patients. the clinical course of b. cepacia infections is variable, but approximately 20% of patients eventually succumb to the cepacia syndrome, which is characterized as a fatal necrotizing pneumonia with bacteremia. the mechanisms that permit b. cepacia to cause bacteremia are not yet known but probably involve sequential penetration of airway barriers. this study evaluated the abilities of different sp ... | 2002 | 12117967 |
| microbial degradation of quinoline by immobilized cells of burkholderia pickettii. | a quinoline-biodegrading microorganism was isolated from activated sludge of coke-oven wastewater treatment plant using quinoline as sole carbon and nitrogen source. it is a gram negative, rod-shaped and aerobic strain, which was identified as burkholderia pickettii. the biodegradation of quinoline was carried out with this isolated strain. analysis by high performance liquid chromatography and gas chromatography/mass spectrum (gc/ms) revealed that 2-hydroxyquinoline (2-oh-q) was the first inter ... | 2002 | 12108721 |
| origins of the 2,4-dinitrotoluene pathway. | the degradation of synthetic compounds requires bacteria to recruit and adapt enzymes from pathways for naturally occurring compounds. previous work defined the steps in 2,4-dinitrotoluene (2,4-dnt) metabolism through the ring fission reaction. the results presented here characterize subsequent steps in the pathway that yield the central metabolic intermediates pyruvate and propionyl coenzyme a (coa). the genes encoding the degradative pathway were identified within a 27-kb region of dna cloned ... | 2002 | 12107140 |
| bacterial contamination of blood components due to burkholderia cepacia contamination from clorhexidine bottles. | 2002 | 12100392 | |
| regioselective enzymatic acylation of methyl shikimate. influence of acyl chain length and solvent polarity on enzyme specificity. | candida antarctica lipase a (cal-a) selectively catalyzes the acylation at the secondary c-4 hydroxyl group of methyl shikimate (2), which possesses three secondary hydroxyl groups, the c-3 allylic one being chemically more reactive. the effect both of the acyl group of the acylating agents and of the solvent polarity has been studied. the selectivity of cal-a is almost complete with acyl donors that possess short chains. however, when acyl donors have longer chains, better results are obtained ... | 2002 | 12098318 |
| infection by ralstonia species in cystic fibrosis patients: identification of r. pickettii and r. mannitolilytica by polymerase chain reaction. | the frequency of respiratory tract infections caused by ralstonia species in persons with cystic fibrosis (cf) and the role of these species in cf pulmonary disease are not well documented. in part, this lack of documentation may be attributed to the difficulty in accurately identifying ralstonia species; r. mannitolilytica and r. pickettii in particular may be misidentified as other closely related species, particularly those of the burkholderia cepacia complex. we used polyphasic analyses to i ... | 2002 | 12095436 |
| characterization of polychlorinated biphenyl-degrading bacteria isolated from contaminated sites in czechia. | biphenyl-utilizing polychlorinated biphenyls (pcb)-degrading bacteria were isolated from sites highly contaminated by pcbs, and their degradation abilities were determined using gc for typical commercial pcb mixtures (delor 103 and delor 106). out of twelve strains which utilized biphenyl as a sole source of carbon and energy, strains pseudomonas alcaligenes kp2 and p. fluorescens kp12, characterized by the biolog identification system and the neferm test, were shown to significantly co-metaboli ... | 2002 | 12094733 |
| comparative antimicrobial spectrum and activity of the desfluoroquinolone bms284756 (t-3811) tested against non-fermentative gram-negative bacilli. | to compare the in vitro activity of bms284756, a desfluoroquinolone, with four fluoroquinolones against 129 pseudomonas aeruginosa strains, 97 stenotrophomonas/burkholderia group strains and 43 acinetobacter spp. strains by three in vitro test methods. | 2002 | 12084101 |
| the cold-active lipase of pseudomonas fragi. heterologous expression, biochemical characterization and molecular modeling. | a recombinant lipase cloned from pseudomonas fragi strain ifo 3458 (pfl) was found to retain significant activity at low temperature. in an attempt to elucidate the structural basis of this behaviour, a model of its three-dimensional structure was built by homology and compared with homologous mesophilic lipases, i.e. the pseudomonas aeruginosa lipase (45% sequence identity) and burkholderia cepacia lipase (38%). in this model, features common to all known lipases have been identified, such as t ... | 2002 | 12084074 |
| burkholderia thailandensis e125 harbors a temperate bacteriophage specific for burkholderia mallei. | burkholderia thailandensis is a nonpathogenic gram-negative bacillus that is closely related to burkholderia mallei and burkholderia pseudomallei. we found that b. thailandensis e125 spontaneously produced a bacteriophage, termed phie125, which formed turbid plaques in top agar containing b. mallei atcc 23344. we examined the host range of phie125 and found that it formed plaques on b. mallei but not on any other bacterial species tested, including b. thailandensis and b. pseudomallei. examinati ... | 2002 | 12081973 |
| family shuffling of a targeted bpha region to engineer biphenyl dioxygenase. | in this work we used a new strategy designed to reduce the size of the library that needs to be explored in family shuffling to evolve new biphenyl dioxygenases (bpdos). instead of shuffling the whole gene, we have targeted a fragment of bpha that is critical for enzyme specificity. we also describe a new protocol to screen for more potent bpdos that is based on the detection of catechol metabolites from chlorobiphenyls. several bpha variants with extended potency to degrade polychlorinated biph ... | 2002 | 12081948 |
| sequence analysis and molecular characterization of a nitrocatechol dioxygenase gene from pseudomonas putida. | a pseudomonas putida capable of degrading polychlorinated biphenyl was also found to transform 4-nitrocatechol to 3-nitro-2-hydroxy-6-oxohexa-2,4-dienoic acid (nhoda). crude cell extract of this bacterium exhibited an enzyme (nitrocatechol dioxygenase, ndo) activity catalyzing this transformation. the gene encoding ndo was cloned in e. coli. the cloned gene (ndo) expressed in e. coli had enzyme activity that degraded not only 4-nitrocatechol but also 4-chlorocatechol, 4-methylcatechol, 2,3-dihyd ... | 2002 | 12081029 |
| sec-dependent and sec-independent translocation of haloacid dehalogenase chd1 of burkholderia cepacia mba4 in escherichia coli. | 2-haloacid dehalogenases are hydrolytic enzymes that cleave the halogen-carbon bond(s) in haloalkanoic acids. we have previously isolated a cryptic haloacid dehalogenase gene from burkholderia cepacia mba4 and expressed it in escherichia coli. this recombinant protein is unusual in having a long leader sequence, a property of periplasmic enzymes. in this paper, we report the functional role of this leader sequence. western blot analyses showed that chd1 is translocated to the periplasm. the resu ... | 2002 | 12076822 |
| an epidemic burkholderia cepacia complex strain identified in soil. | life threatening infection with species of the burkholderia cepacia complex frequently occurs as a result of cross infection among individuals with cystic fibrosis. stringent infection control measures have decreased but not eliminated such infection in this vulnerable population, implying that non-patient reservoirs contribute to ongoing acquisition. however, strains common to both the natural environment and patients with cystic fibrosis have not yet been described. by use of various genotypin ... | 2002 | 12076559 |
| building blocks for the solution phase synthesis of oligonucleotides: regioselective hydrolysis of 3',5'-di-o-levulinylnucleosides using an enzymatic approach. | a short and convenient synthesis of 3'- and 5'-o-levulinyl-2'-deoxynucleosides has been developed from the corresponding 3',5'-di-o-levulinyl derivatives by regioselective enzymatic hydrolysis, avoiding several tedious chemical protection/deprotection steps. thus, candida antartica lipase b (cal-b) was found to selectively hydrolyze the 5'-levulinate esters, furnishing 3'-o-levulinyl-2'-deoxynucleosides 3 in >80% isolated yields. on the other hand, immobilized pseudomonas cepacia lipase (psl-c) ... | 2002 | 12076150 |
| aerobic degradation of 2,4,6-trichlorophenol by a microbial consortium - selection and characterization of microbial consortium. | a microbial consortium that efficiently degrades 2,4,6-tcp (2,4,6-trichlorophenol), as the sole source of carbon and energy under aerobic conditions was selected from municipal activated sludge. six bacterial strains, designated s(1), s(2), s(3), s(4), s(5) and s(6), were isolated from the selected consortium and five were identified as sphingomonas paucimobilis (s(2), s(3)), burkholderia cepacia(s(4)), chryseomonas luteola (s(5)) and vibrio metschnikovii (s(6)). after prolonged cultivation foll ... | 2002 | 12073141 |
| identification of a general secretory pathway in a human isolate of burkholderia vietnamiensis (formerly b. cepacia complex genomovar v) that is required for the secretion of hemolysin and phospholipase c activities. | members of the burkholderia cepacia complex can secrete proteases, lipases, and hemolysins. we report in this study the identification of a general secretory pathway present in a b. vietnamiensis (formerly genomovar v) clinical isolate, which is required for the efficient secretion of phospholipase c and hemolysin activities. southern blot hybridization experiments revealed that this general secretion pathway is highly conserved among the different genomovars of the b. cepacia complex and is hom ... | 2002 | 12071681 |
| [yearly changes in antibacterial activities of cefozopran against various clinical isolates between 1996 and 2000--ii. gram-negative bacteria]. | the in vitro antibacterial activities of cefozopran (czop), an agent of cephems, against various clinical isolates obtained between 1996 and 2000 were yearly evaluated and compared with those of other cephems, oxacephems, and carbapenems. thirty-two species 2,697 strains of gram-negative bacteria were isolated from the clinical materials annually collected from january to december, and consisted of moraxella subgenus branhamella catarrhalis (n = 125), escherichia coli (n = 250), citrobacter freu ... | 2002 | 12071094 |
| aciduric proteobacteria isolated from ph 2.9 soil. | acidic (ph 2.9) soil was used as an inoculum to culture heterotrophic bacteria at ph values of 3-4. four isolates were obtained; on the basis of 16s rdna sequence, they were shown to be members of the beta- and gamma-proteobacteria. the three isolates that were most closely related to burkholderia spp. had simple nutritional requirements and could grow in glucose-mineral salts media; two of these used a broad array of organic substrates. the 16s rdna sequence of the fourth isolate was most simil ... | 2002 | 12070771 |
| characterization of burkholderia pseudomallei infection and identification of novel virulence factors using a caenorhabditis elegans host system. | the environmental saphrophyte burkholderia pseudomallei is the causative agent of melioidosis, a systemic, potentially life-threatening condition endemic to many parts of south-east asia and northern australia. we have used the soil nematode caenorhabditis elegans as a model host to characterize the mechanisms by which this bacterium mounts a successful infection. we find that c. elegans is susceptible to a broad range of burkholderia species, and that the virulence mechanisms used by this patho ... | 2002 | 12068805 |
| efficient chemoenzymatic synthesis of (s)- and (r)-5-(1-aminoethyl)-2-(cyclohexylmethoxy)benzamide: key intermediate for src-sh2 inhibitor. | a facile chemoenzymatic synthesis of both the s and r forms of 5-(1-aminoethyl)-2-(cyclohexylmethoxy)benzamide a key intermediate of non-peptidic src sh2 inhibitors is described. both the enantiomers were synthesized in high optical purity (>99% ee) by reduction followed by lipase-mediated acylation of the precursor 6 in one-pot. immobilized pseudomonas cepacia lipase offered high degree of enantioselectivity with spontaneity. | 2002 | 12067549 |
| analogous cytokine responses to burkholderia pseudomallei strains contrasting in virulence correlate with partial cross-protection in immunized mice. | cytokine mrna levels were assessed in burkholderia pseudomallei-susceptible balb/c mice and b. pseudomallei-resistant c57bl/6 mice following administration of a sublethal dose of less virulent (lv) b. pseudomallei, a candidate immunogen tested for protection against a highly virulent (hv) challenge. compared on the basis of the bacterial loads, the cytokine patterns induced by hv and lv b. pseudomallei were similar, involving gamma interferon, interleukin-10, and other cytokines. partial cross-p ... | 2002 | 12065542 |
| microwave-assisted rapid characterization of lipase selectivities. | a rapid screening procedure for characterization of lipase selectivities using microwaves was developed. the rate of reaction of various commercial lipases (porcine pancreas, mucor miehei, candida rugosa, pseudomonas cepacia) as well as lipases from laboratory isolates-bacillus stearothermophilus and burkholderia cepacia rgp-10 for triolein hydrolysis was 7- to 12-fold higher in a microwave oven as compared to that by ph stat. the esterification of sucrose/methanol and ascorbic acid with differe ... | 2002 | 12062110 |
| enhanced resistance to seed-transmitted bacterial diseases in transgenic rice plants overproducing an oat cell-wall-bound thionin. | bacterial attack is a serious agricultural problem for growth of rice seedlings in the nursery and field. the thionins purified from seed and etiolated seedlings of barley are known to have antimicrobial activity against necrotrophic pathogens; however, we found that no endogenous rice thionin genes alone are enough for resistance to two major seed-transmitted phytopathogenic bacteria, burkholderia plantarii and b. glumae, although rice thionin genes constitutively expressed in coleoptile, the t ... | 2002 | 12059099 |
| burkholderia anthina sp. nov. and burkholderia pyrrocinia, two additional burkholderia cepacia complex bacteria, may confound results of new molecular diagnostic tools. | nineteen burkholderia cepacia-like isolates of human and environmental origin could not be assigned to one of the seven currently established genomovars using recently developed molecular diagnostic tools for b. cepacia complex bacteria. various genotypic and phenotypic characteristics were examined. the results of this polyphasic study allowed classification of the 19 isolates as an eighth b. cepacia complex genomovar (burkholderia anthina sp. nov.) and to design tools for its identification in ... | 2002 | 12052570 |
| a liquid-based method for the assessment of bacterial pathogenicity using the nematode caenorhabditis elegans. | caenorhabditis elegans has previously been used as an alternative to mammalian models of infection with bacterial pathogens. we have developed a liquid-based assay to measure the effect of bacteria on the feeding ability of c. elegans. using this assay we have shown that pseudomonas aeruginosa strain pa14, burkholderia pseudomallei and yersinia pestis were able to inhibit feeding of c. elegans strain n2. an increase in sensitivity of the assay was achieved by using c. elegans mutant phm-2, in pl ... | 2002 | 12044672 |
| activity of five fluoroquinolones against 71 isolates of burkholderia pseudomallei. | 2002 | 12039903 | |
| characterization of unusual bacteria isolated from respiratory secretions of cystic fibrosis patients and description of inquilinus limosus gen. nov., sp. nov. | using a polyphasic approach (including cellular protein and fatty acid analysis, biochemical characterization, 16s ribosomal dna sequencing, and dna-dna hybridizations), we characterized 51 bacterial isolates recovered from respiratory secretions of cystic fibrosis (cf) patients. our analyses showed that 24 isolates belong to taxa that have so far not (or only rarely) been reported from cf patients. these taxa include acinetobacter sp., bordetella hinzii, burkholderia fungorum, comamonas testost ... | 2002 | 12037065 |
| catabolic and genetic diversity of degradative bacteria from fuel-hydrocarbon contaminated aquifers. | a bstractsubsurface sediments were recovered from two aquifers contaminated with petroleum hydrocarbons in order to isolate and characterize indigenous microorganisms capable of biodegrading fuel-related compounds. these sediments had been previously studied using catabolic gene probes providing putative demonstration of significant biodegradation potential. based on 16s rdna sequence analysis, the isolates were phylogenetically similar to common soil microorganisms, including members of the gen ... | 2000 | 12035098 |
| a n2-fixing endophytic burkholderia sp. associated with maize plants cultivated in mexico. | in the frame of a survey of potentially endophytic n2-fixing burkholderia associated with maize in mexico, its country of origin, the soil of an indigenous maize field near oaxaca was studied. under laboratory conditions, plant seedlings of two ancient maize varieties were used as a trap to select endophyte candidates from the soil sample. among the n2 fixers isolated from inside plant tissues and able to grow on pcat medium, the most abundant isolates belonged to genus burkholderia (api 20ne, r ... | 2002 | 12030700 |
| identification and evolutionary analysis of putative cytoplasmic mcpa-like protein in a bacterial strain living in symbiosis with a mycorrhizal fungus. | in this paper we report the identification and characterization of a dna region containing putative mcpa-like gene coding for a methyl accepting chemotaxis protein (mcp) and belonging to a burkholderia endosymbiont of the arbuscular mycorrhizal fungus gigaspora margarita. a genomic library of total dna extracted from the fungal spores, representative of the bacterial genome, was used to investigate the prokaryotic genome. pcr experiments with primers designed on the burkholderia mcpa-like gene a ... | 2002 | 12029363 |
| burkholderia cepacia in cystic fibrosis: implications for nursing practice. | burkholderia cepacia (b. cepacia) has emerged in the last 15 years as a dangerous pathogen among individuals with cystic fibrosis (cf). although this pathogen affects a relatively small percentage of the total cf population, it has had an enormous impact on the cf community because of the increased morbidity and mortality associated with it. many cf centers have instituted stringent infection control practices in an effort to prevent the spread of this bacterium. nurses who work with cf patients ... | 2000 | 12026400 |
| pcr cloning of polyhydroxyalkanoate biosynthesis genes from burkholderia caryophylli and their functional expression in recombinant escherichia coli. | the pcr cloning strategy for type ii polyhydroxyalkanoate (pha) biosynthesis genes established previously for pseudomonas was successfully applied to burkholderia caryophylli strain as 1.2741. the whole pha locus containing pha synthase genes phac1, phac2 and pha depolymerase gene phaz was cloned. the complete open reading frames of phac1(bc), phac2(bc) and phaz(bc) were identified. sequence analyses of the phac1(bc), phaz(bc) and phac2(bc) showed more than 77.7%, 73.7% and 68.5% identities comp ... | 2002 | 12023076 |
| antibacterial activities and characterization of novel inhibitors of lpxc. | lipid a is the hydrophobic anchor of lipopolysaccharide (lps) and forms the major lipid component of the outer monolayer of the outer membrane of gram-negative bacteria. lipid a is required for bacterial growth and virulence, and inhibition of its biosynthesis is lethal to bacteria. udp-3-o-(r-3-hydroxymyristoyl)-n-acetylglucosamine deacetylase (lpxc) is a metalloenzyme that catalyzes the second step in the biosynthesis of lipid a. inhibitors of lpxc have previously been shown to have antibiotic ... | 2002 | 12019092 |
| melioidosis of the head and neck. | melioidosis is a potentially deadly infection that can affect any organ system. reports of melioidosis of the ent/head and neck region are relatively uncommon. four cases are presented: (i) parotid abscess evolving into necrotising fasciitis, (ii) acute sinusitis and parapharyngeal cellulitis resulting in upper airway obstruction, (iii) acute suppurative lymphadenitis (iv) and chronic suppurative otitis media causing meningoencephalitis. three of the four cases are believed to be unique, as a li ... | 2001 | 12014768 |
| effects of two different application methods of burkholderia ambifaria mci 7 on plant growth and rhizospheric bacterial diversity. | in order to acquire a better understanding of the effects of the different delivery modes of bacterial inoculants on plant growth and on the community structure of rhizosphere bacterial populations, burkholderia ambifaria mci 7 (formerly b. cepacia mci 7) was inoculated into the rhizosphere of maize plants by either seed adhesion or incorporation into soil. plant growth was evaluated at different inoculum concentrations. the community structure of rhizosphere bacterial populations was evaluated ... | 2002 | 12010130 |
| fluorescent inhibitors reveal solvent-dependent micropolarity in the lipid binding sites of lipases. | triacylglycerol analogue p-nitrophenyl phosphonates specifically react with the active-site serine of lipolytic enzymes to give covalent lipase-inhibitor complexes, mimicking the first transition state which is involved in lipase-mediated ester hydrolysis. here we report on a new type of phosphonate inhibitors containing a polarity-sensitive fluorophore to monitor micropolarity around the active site of the enzyme in different solvents. the respective compounds are hexyl and methyl dimethylamino ... | 2002 | 12009403 |