Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| cost utility of palivizumab prophylaxis among pre-term infants in the united states: a national policy perspective. | the cost-effectiveness of palivizumab has previously been reported among certain guideline-eligible, high-risk premature infants in medicaid. because guideline authorities base decisions on a national perspective, the economic model of palivizumab was adapted to include all infants, that is, public and privately insured patients (60% of palivizumab use is public, 40% is private). | 2012 | 22574798 |
| il1rl1 gene variants and nasopharyngeal il1rl-a levels are associated with severe rsv bronchiolitis: a multicenter cohort study. | targets for intervention are required for respiratory syncytial virus (rsv) bronchiolitis, a common disease during infancy for which no effective treatment exists. clinical and genetic studies indicate that il1rl1 plays an important role in the development and exacerbations of asthma. human il1rl1 encodes three isoforms, including soluble il1rl1-a, that can influence il33 signalling by modifying inflammatory responses to epithelial damage. we hypothesized that il1rl1 gene variants and soluble il ... | 2012 | 22574108 |
| production of chemokines in respiratory syncytial virus infection with central nervous system manifestations. | respiratory syncytial virus (rsv) infection in children can be associated with acute encephalopathy. however, the roles of cytokines in the cerebrospinal fluid (csf) of such patients remain unevaluated. in this study, a profile of 17 cytokines was determined for eight rsv-infected children with neurological complications. in one patient with high levels of 13 cytokines, a cytokine storm was considered to have occurred. interleukin (il)-6, il-8, monocyte chemoattractant protein (mcp)-1, and macro ... | 2012 | 22572852 |
| meteorologic conditions and respiratory syncytial virus activity. | the factors that determine the initiation and persistence of yearly epidemics of respiratory syncytial virus (rsv) bronchiolitis are not well known. a limited number of studies have found that meteorologic conditions could be associated with rsv activity. | 2012 | 22572747 |
| respiratory syncytial virus persistence in macrophages downregulates intercellular adhesion molecule-1 expression and reduces adhesion of non-typeable haemophilus influenzae. | persistence of respiratory syncytial virus (rsv) has been associated with episodes of chronic obstructive pulmonary disease (copd); furthermore, co-infection of rsv with non-typeable haemophilus influenzae (nthi) is increasingly recognized as a cause of exacerbations of copd. | 2012 | 22572178 |
| recent developments with live-attenuated recombinant paramyxovirus vaccines. | there is no vaccine currently approved for paramyxovirus-induced respiratory diseases in humans, despite their major clinical importance. we review the development and evaluation of new vaccine strategies based on live-attenuated chimeric and recombinant vaccines against human respiratory syncytial virus, human metapneumovirus and human parainfluenza viruses types 1 to 3, which are significant causes of upper and lower tract respiratory diseases. most promising strategies are based on virus atte ... | 2013 | 22570186 |
| frequency and clinical outcome of respiratory viral infections and mixed viral-bacterial infections in children with cancer, fever and neutropenia. | the role of respiratory viral infections (rvis) as a cause of overall fever and neutropenia (fn) episodes in children with cancer has been less characterized than bacterial infections. we conducted a study aimed to determine the frequency of rvi in children with low compared with high risk for invasive bacterial infection (ibi) fn episodes and compare the clinical outcome of rvi and mixed rv-bacterial infections. | 2012 | 22565292 |
| epidemiology of human metapneumovirus in a pediatric long-term care facility. | viral respiratory pathogens cause outbreaks in pediatric long-term care facilities (ltcfs), but few studies have used viral diagnostic testing to identify the causative pathogens. we describe the use of such testing during a prolonged period of respiratory illness and elucidate the epidemiology of human metapneumovirus (hmpv) at our ltcf. | 2012 | 22561708 |
| cost-effectiveness of potential infant vaccination against respiratory syncytial virus infection in the netherlands. | respiratory syncytial virus (rsv) infection is one of the major causes of respiratory illness in infants, infecting virtually every child before the age of 2 years. currently, several phase 1 trials with rsv vaccines in infants are ongoing or have been completed. as yet, no efficacy estimates are available for these vaccine candidates. nevertheless, cost-effectiveness estimates might be informative to enable preliminary positioning of an rsv vaccine. | 2012 | 22561315 |
| pediatric rsv infection during two winter seasons in british columbia: a role for subgroup analysis in young children? | using a panel of eight monoclonal antibodies directed against the g, f and np proteins of respiratory syncytial virus, 167 virus isolates from nasopharyngeal washing cultures at british columbia children's hospital during two consecutive epidemics were subgrouped. slides made and frozen at the time of virus isolation or prepared from recovered frozen passage material, were assayed by indirect immunofluorescence. of 85 strains tested in 1987-88, 54 (64%) were subgroup a, and 31 (36%) subgroup b. ... | 1990 | 22553451 |
| successful management of sequential pulmonary infections in a cardiac transplant recipient. | a case of a cardiac allograft recipient who had an initial combined pulmonary infection with cytomegalovirus, aspergillus fumigatus and nocardia asteroides, successfully treated with liposomal amphotericin b and sulfisoxazole and followed by an episode of respiratory syncytial virus pneumonitis, is presented. this case illustrates the role of computed tomographic imaging in the recognition, diagnosis and monitoring of complex opportunistic pulmonary infections and the benefits of liposomal ampho ... | 1990 | 22553447 |
| comparison of risk factors for human metapneumovirus and respiratory syncytial virus disease severity in young children. | human metapneumovirus (hmpv) and respiratory syncytial virus (rsv) are leading pediatric pathogens. however, risk factors for severe hmpv disease remain unknown. we comparatively assessed environmental, host, and viral determinants for severe hmpv and rsv infections. | 2012 | 22551815 |
| antibodies to the central conserved region of respiratory syncytial virus (rsv) g protein block rsv g protein cx3c-cx3cr1 binding and cross-neutralize rsv a and b strains. | respiratory syncytial virus (rsv) is a primary cause of severe lower respiratory tract disease in infants, young children, and the elderly worldwide, and despite decades of effort, there remains no safe and effective vaccine. rsv modifies the host immune response during infection by cx3c chemokine mimicry adversely affecting pulmonary leukocyte chemotaxis and cx3cr1+ rsv-specific t-cell responses. in this study we investigated whether immunization of mice with rsv g protein polypeptides from str ... | 2012 | 22551066 |
| aetiology of community-acquired pneumonia among adults in an h1n1 pandemic year: the role of respiratory viruses. | this study aimed to determine the aetiology of community-acquired pneumonia (cap) by adding polymerase chain reaction (pcr) to conventional methods and to describe the clinical and laboratory features between patients with bacterial pneumonia (bp) and viral pneumonia (vp). adults with cap admitted from november 2009 to october 2010 were included. demographics, comorbidities, severity and clinical features were recorded. conventional microbiological methods included blood and sputum cultures, acu ... | 2012 | 22549730 |
| a comparative study of respiratory syncytial virus (rsv) prophylaxis in premature infants within the canadian registry of palivizumab (caress). | we examined the dosing regimens, compliance, and outcomes of premature infants who received palivizumab within the canadian registry of palivizumab (caress). infants receiving ≥1 dose of palivizumab during the 2006-2011 respiratory syncytial virus (rsv) seasons were recruited across 30 sites. respiratory illness events were captured monthly. infants ≤32 completed weeks gestational age (ga) (group 1) were compared to 33-35 completed weeks ga infants (group 2) following prophylaxis. in total, 6,65 ... | 2012 | 22546928 |
| respiratory syncytial virus infection increases regulated on activation normal t cell expressed and secreted and monocyte chemotactic protein 1 levels in serum of patients with asthma and in human monocyte cultures. | respiratory syncytial virus (rsv) infection is associated to episodic exacerbations of asthma involving alveolar macrophages and chemokine production. | 2012 | 22541401 |
| baculovirus-based vaccine displaying respiratory syncytial virus glycoprotein induces protective immunity against rsv infection without vaccine-enhanced disease. | respiratory syncytial virus (rsv) is a major cause of severe lower respiratory tract diseases in infancy and early childhood. despite its importance as a pathogen, there is no licensed vaccine against rsv yet. the attachment glycoprotein (g) of rsv is a potentially important target for protective antiviral immune responses. recombinant baculovirus has been recently emerged as a new vaccine vector, since it has intrinsic immunostimulatory properties and good bio-safety profile. | 2012 | 22536165 |
| role of human tlr4 in respiratory syncytial virus-induced nf-κb activation, viral entry and replication. | tlrs play a key role in innate immune defenses. it was previously reported that purified respiratory syncytial virus (rsv) fusion protein elicits an inflammatory response in hematopoietic cells, which required expression of tlr4 and its co-receptor cd14. however, a biological role of tlr4 in immunity to rsv, as initially proposed, has remained inconclusive and controversial. here, we directly assess the role of human tlr4 and its co-receptors in nf-κb activation, viral entry and replication usin ... | 2012 | 22535679 |
| respiratory syncytial virus lower respiratory tract infection in a pediatric liver transplant recipient treated with oral ribavirin. | the mainstay of therapy for rsv disease is supportive care, although aerosolized ribavirin has been used to treat infants and young children with severe lower respiratory tract infections. aerosolized ribavirin has adverse effects, high cost and teratogenic potential. we report the case of a pediatric liver transplant recipient diagnosed with lower respiratory rsv infection, who was successfully treated with oral ribavirin. | 2012 | 22533837 |
| spectrum of respiratory viruses in children with community-acquired pneumonia. | community-acquired pneumonia (cap) remains a significant cause for childhood morbidity worldwide. we designed a study with the objective of describing the frequency of respiratory viruses, especially rhinovirus (rv), human metapneumovirus (hmpv) and human bocavirus (hbov) in hospitalized children with cap. | 2012 | 22531244 |
| epidemiology of respiratory syncytial virus infection in infants born at less than thirty-five weeks of gestational age. | the aims of this study were to observe the respiratory syncytial virus (rsv) hospitalization rate and to identify the risk factors for hospitalization for rsv infection among infants in korea born at <35 weeks of gestational age and who had not previously received palivizumab. | 2012 | 22531234 |
| bats host major mammalian paramyxoviruses. | the large virus family paramyxoviridae includes some of the most significant human and livestock viruses, such as measles-, distemper-, mumps-, parainfluenza-, newcastle disease-, respiratory syncytial virus and metapneumoviruses. here we identify an estimated 66 new paramyxoviruses in a worldwide sample of 119 bat and rodent species (9,278 individuals). major discoveries include evidence of an origin of hendra- and nipah virus in africa, identification of a bat virus conspecific with the human ... | 2012 | 22531181 |
| rates of hospitalizations for respiratory syncytial virus, human metapneumovirus, and influenza virus in older adults. | we performed a prospective study to determine the disease burden of respiratory syncytial virus (rsv) and human metapneumovirus (hmpv) in older adults in comparison with influenza virus. | 2012 | 22529314 |
| increase in use of non-invasive ventilation for infants with severe bronchiolitis is associated with decline in intubation rates over a decade. | to redress the paucity of studies evaluating non-invasive respiratory support in bronchiolitis patients. | 2012 | 22527081 |
| prospective evaluation of clinical scoring systems in infants with bronchiolitis admitted to the intensive care unit. | the objective of this investigation was to compare different scoring systems to assess the severity of illness in infants with bronchiolitis admitted to a tertiary paediatric intensive care unit (picu). over an 18-year period (1990-2007), infants with bronchiolitis aged up to 12 months and admitted to the picu were prospectively scored using the pediatric risk of mortality iii (prism iii) score, the organ system failure (osf) score and the acute physiologic score for children (apsc) within 24 h. ... | 2012 | 22526870 |
| the epidemiology and clinical characteristics of respiratory syncytial virus infection in children at a public pediatric referral hospital in mexico. | the aim of this study was to determine the epidemiological and clinical characteristics of children with respiratory syncytial virus (rsv) treated at a public referral children's hospital in mexico. | 2012 | 22525227 |
| residential crowding and severe respiratory syncytial virus disease among infants and young children: a systematic literature review. | the objective of this literature review was to determine whether crowding in the home is associated with an increased risk of severe respiratory syncytial virus (rsv) disease in children younger than 5 years. | 2012 | 22520624 |
| [preliminary analysis on respiratory syncytial virus identified in children with acute respiratory infections in tibet autonomous region, china]. | to understand the role of respiratory syncytial virus (rsv) in children with acute respiratory infections (ari) in tibet autonomous region and the contribution of two major groups of rsv, nasopharyngeal aspirates (npa) were collected from hospitalized children with ari in department of pediatrics, tibet people's hospital in lasa, tibet from april to july in 2011 and tested for seven common respiratory viruses and human metapneumovirus (hmpv) by direct immunofluorescence assay (dfa). total rnas w ... | 2012 | 22519168 |
| comparison of the luminex xtag rvp fast assay and the idaho technology filmarray rp assay for detection of respiratory viruses in pediatric patients at a cancer hospital. | respiratory viruses are increasingly recognized as serious causes of morbidity and mortality in immunocompromised patients. the rapid and sensitive detection of respiratory viruses is essential for the early diagnosis and administration of appropriate antiviral therapy, as well as for the effective implementation of infection control measures. we compared the performance of two commercial assays, xtag rvp fast (luminex diagnostics, toronto, canada) and filmarray rvp (fa rvp; idaho technology, sa ... | 2012 | 22518855 |
| managing the morbidity associated with respiratory viral infections in children with congenital heart disease. | children with congenital heart disease (chd) are at risk for increased morbidity from viral lower respiratory tract infections because of anatomical cardiac lesions than can worsen an already compromised respiratory status. respiratory syncytial virus (rsv) remains an important pathogen in contributing toward the morbidity in this population. although the acute treatment of rsv largely remains supportive, the development of monoclonal antibodies, such as palivuzumab, has reduced the rsv-related ... | 2012 | 22518179 |
| viral epidemiology and severity of respiratory infections in infants in 2009: a prospective study. | viral respiratory infections are common in infants and can be severe. the new pandemic influenza virus h1n1v2009 was feared to cause particularly severe outcomes. | 2012 | 22517337 |
| radiological findings in 210 paediatric patients with viral pneumonia: a retrospective case study. | the objective of this study was to assess the radiological presentations of different types of viral pneumonia in children. | 2012 | 22514104 |
| respiratory syncytial virus in indonesian children. | 2012 | 22510994 | |
| risk factors for hospitalization with lower respiratory tract infections in children in rural alaska. | lower respiratory tract infections (lrtis) are a major cause of morbidity for children worldwide and particularly for children from developing and indigenous populations. in this study, we evaluated risk factors for hospitalization with lrti in a region in southwest alaska. | 2012 | 22508919 |
| liquid chromatographic resolution of 3-amino-1,4-benzodiazepin-2-ones on crown ether-based chiral stationary phases. | 3-amino-5-phenyl (or 5-methyl)-1,4-benzodiazepin-2-ones, which are chiral precursors of anti-respiratory syncytial virus active agents, were resolved on three different chiral stationary phases (csps) based on (+)-(18-crown-6)-2,3,11,12-tetracarboxylic acid or (3,3'-diphenyl-1,1'-binaphthyl)-20-crown-6. among the three csps, the csp that is based on (3,3'-diphenyl-1,1'-binaphthyl)-20-crown-6 and containing residual silanol group-protecting n-octyl groups on the silica surface was found to be mos ... | 2012 | 22508444 |
| chest physical therapy is effective in reducing the clinical score in bronchiolitis: randomized controlled trial. | to evaluate the effectiveness of chest physical therapy (cp) in reducing the clinical score in infants with acute viral bronchiolitis (avb). | 2012 | 22499404 |
| [effective infliximab treatment for a recurrent type of acute intestinal graft-versus-host disease accompanied by steroid-induced depression]. | a 22-year-old man with chronic active epstein-barr virus infection underwent allogeneic bone marrow transplantation (allo-bmt) from an hla two allele-mismatched unrelated donor. ten months after allo-bmt, he developed protein-losing enteropathy following a respiratory syncytial virus infection. a diagnosis of a recurrent type of acute graft-versus-host disease (gvhd) was made based on the histopathological findings, such as the infiltration of t lymphocytes into the superficial epithelium and cr ... | 2012 | 22499056 |
| broad respiratory virus detection in infants hospitalized for bronchiolitis by use of a multiplex rt-pcr dna microarray system. | newly available molecular tools allow a sensitive detection of a broad panel of viruses in respiratory tract specimens. in the present study, the application of a multiplex rt-pcr dna microarray in diagnosis and epidemiological survey of viral infections in infants hospitalized for bronchiolitis was assessed. one hundred and thirty-eight nasopharyngeal aspirates collected from october 2007 to september 2008 were tested by direct immunofluorescence and viral culture, a combination of referenced r ... | 2012 | 22499022 |
| host response to mechanical ventilation for viral respiratory tract infection. | respiratory syncytial virus (rsv) bronchiolitis causes severe respiratory tract infection in infants, frequently necessitating mechanical ventilatory support. however, life-saving, mechanical ventilation aggravates lung inflammation. we set up a model to dissect the host molecular response to mechanical ventilation in rsv infection. furthermore, the response to induced hypercapnic acidosis, reported to dampen the inflammatory response to mechanical ventilation in non-infectious models, was asses ... | 2012 | 22496321 |
| hospitalizations associated with influenza and respiratory syncytial virus in the united states, 1993-2008. | age-specific comparisons of influenza and respiratory syncytial virus (rsv) hospitalization rates can inform prevention efforts, including vaccine development plans. previous us studies have not estimated jointly the burden of these viruses using similar data sources and over many seasons. | 2012 | 22495079 |
| respiratory viruses in children hospitalized for acute lower respiratory tract infection in ghana. | acute respiratory tract infections are one of the major causes of morbidity and mortality among young children in developing countries. information on the viral aetiology of acute respiratory infections in developing countries is very limited. the study was done to identify viruses associated with acute lower respiratory tract infection among children less than 5 years. | 2012 | 22490115 |
| [changes and the clinical significance of cd4⁺ cd25⁺ regulatory t cells and th17 cells in peripheral blood of infants with respiratory syncytial virus bronchiolitis]. | to observe the percentages of cd4(+);cd25(+); regulatory t cells (tregs) and th17 cells and the levels of il-10, tgf-β and il-17 in peripheral blood of infants with respiratory syncytial virus (rsv) bronchiolitis. the relationship between above cells, cytokines and rsv bronchiolitis was determined. | 2012 | 22482417 |
| the respiratory syncytial virus g protein conserved domain induces a persistent and protective antibody response in rodents. | respiratory syncytial virus (rsv) is an important cause of severe upper and lower respiratory disease in infants and in the elderly. there are 2 main rsv subtypes a and b. a recombinant vaccine was designed based on the central domain of the rsv-a attachment g protein which we had previously named g2na (aa130-230). here we evaluated immunogenicity, persistence of antibody (ab) response and protective efficacy induced in rodents by: (i) g2na fused to dt (diphtheria toxin) fragments in cotton rats ... | 2012 | 22479601 |
| respiratory syncytial virus prophylaxis in special populations: is it something worth considering in cystic fibrosis and immunosuppression? | respiratory syncytial virus (rsv) bronchiolitis is the leading cause of infant hospitalization in the united states. prophylaxis with palivizumab is effective in reducing rsv hospitalizations in premature infants and in infants or children with chronic lung disease or congenital heart disease. patients with cf or those who are immunocompromised may be at increased risk for rsv infection-related complications; hence, prophylaxis may prove beneficial to these populations. the extent of palivizumab ... | 2011 | 22477829 |
| respiratory syncytial virus pathophysiology and affect of palivizumab in special populations: cystic fibrosis and immunosuppression. | 2011 | 22477828 | |
| prospective multicenter study of viral etiology and hospital length of stay in children with severe bronchiolitis. | to determine whether hospital length of stay(los) for acute bronchiolitis is influenced by the infecting pathogen. | 2012 | 22473882 |
| genetic variability of human respiratory syncytial virus a strains circulating in ontario: a novel genotype with a 72 nucleotide g gene duplication. | human respiratory syncytial virus (hrsv) is the main cause of acute lower respiratory infections in children under 2 years of age and causes repeated infections throughout life. we investigated the genetic variability of rsv-a circulating in ontario during 2010-2011 winter season by sequencing and phylogenetic analysis of the g glycoprotein gene.among the 201 consecutive rsv isolates studied, rsv-a (55.7%) was more commonly observed than rsv-b (42.3%). 59.8% and 90.1% of rsv-a infections were am ... | 2012 | 22470426 |
| performance of the luminex xtag respiratory viral panel fast in a clinical laboratory setting. | the aim of the study was to develop a real-time rt-pcr for the detection of enteroviruses (evs) and rhinoviruses (rvs) and to assess the performance of the xtag rvp fast assay in comparison to a direct fluorescent assay (dfa), a real-time rt-pcr assay for the detection of respiratory syncytial virus (rsv) and human metapneumovirus (hmpv), and the ev/rv rt-pcr assay developed in this study. the performance of the rvp fast assay was assessed in the analysis of 373 nasopharyngeal samples. for the v ... | 2012 | 22465255 |
| influenza and respiratory syncytial virus infections in british hajj pilgrims. | viral respiratory infections including influenza and respiratory syncytial virus (rsv) have been reported during the hajj among international pilgrims. to help establish the burden of these infections at the hajj, we set up a study to confirm these diagnoses in symptomatic british pilgrims who attended the 2005 hajj. uk pilgrims with symptoms of upper respiratory tract infection (urti) were invited to participate; after taking medical history, nasal swabs were collected for point-of-care testing ... | 2008 | 22460211 |
| antiviral ifn-γ responses of monocytes at birth predict respiratory tract illness in the first year of life. | viral respiratory tract infections are the leading cause of acute illness during infancy and are closely linked to chronic inflammatory airway diseases later in life. however, the determinants of susceptibility to acute respiratory tract infections still need to be defined. | 2012 | 22460071 |
| [clinical characteristics of bronchiolitis obliterans in pediatric patients]. | to analyze the clinical characteristics, image findings, laboratory examination, the therapeutic methods and clinical outcomes of bronchiolitis obliterans (bo) in pediatric patients. | 2012 | 22455631 |
| one-step multiplex real time rt-pcr for the detection of bovine respiratory syncytial virus, bovine herpesvirus 1 and bovine parainfluenza virus 3. | detection of respiratory viruses in veterinary species has traditionally relied on virus detection by isolation or immunofluorescence and/or detection of circulating antibody using elisa or serum neutralising antibody tests. multiplex real time pcr is increasingly used to diagnose respiratory viruses in humans and has proved to be superior to traditional methods. bovine respiratory disease (brd) is one of the most common causes of morbidity and mortality in housed cattle and virus infections can ... | 2012 | 22455597 |
| identifying targets in the hunt for effective respiratory syncytial virus interventions. | respiratory syncytial virus (rsv) is a major cause of human infections worldwide. there is currently no effective vaccine or antiviral therapy available for widespread clinical use; prophylaxis with anti-rsv antibodies is used in only a small percentage of potential recipients. new targets for effective rsv interventions are needed. previous anti-rsv intervention strategies have focused on targeting aspects of the virus, an approach that can lead to the emergence of resistant rsv strains. increa ... | 2012 | 22455493 |
| determinants of asthma after severe respiratory syncytial virus bronchiolitis. | the development of asthma after respiratory syncytial virus (rsv) bronchiolitis has been demonstrated in case-control studies, although the determinants of post-rsv asthma remain undefined. | 2012 | 22444510 |
| prevention of acute otitis media using currently available vaccines. | acute otitis media (aom) is common in infants and children. although approximately two-thirds of cases are due to bacteria, almost all of the episodes are preceded by upper respiratory viral infection. several viruses, among which respiratory syncytial virus is the most common, are involved in the determination of aom. however, a significant number of aom cases are associated with influenza infection, and influenza viruses are among the most frequently found respiratory viruses in the middle ear ... | 2012 | 22439723 |
| quantitative trait loci associated with the immune response to a bovine respiratory syncytial virus vaccine. | infectious disease is an important problem for animal breeders, farmers and governments worldwide. one approach to reducing disease is to breed for resistance. this linkage study used a charolais-holstein f2 cattle cross population (n = 501) which was genotyped for 165 microsatellite markers (covering all autosomes) to search for associations with phenotypes for bovine respiratory syncytial virus (brsv) specific total-igg, igg1 and igg2 concentrations at several time-points pre- and post-brsv va ... | 2012 | 22438944 |
| evaluation of pneumonia virus of mice as a possible human pathogen. | pneumonia virus of mice (pvm), a relative of human respiratory syncytial virus (rsv), causes respiratory disease in mice. there is serologic evidence suggesting widespread exposure of humans to pvm. to investigate replication in primates, african green monkeys (agm) and rhesus macaques (n = 4) were inoculated with pvm by the respiratory route. virus was shed intermittently at low levels by a subset of animals, suggesting poor permissiveness. pvm efficiently replicated in cultured human cells and ... | 2012 | 22438539 |
| the outcome after severe bronchiolitis is related to gender and virus. | the association between bronchiolitis in the first year of life and subsequent asthma, atopy, airway obstruction and bronchial hyper-responsiveness (bhr) is unsettled. genetic predispositions, pre-morbid lung function, environmental interactions and altered immunological responses are risk factors that have been studied. the aim of this study was to assess lung function, bhr and the occurrence of asthma and atopy 11 yr after hospitalization for bronchiolitis in the first year of life, particular ... | 2012 | 22435682 |
| cost-effectiveness analysis of palivizumab among pre-term infant populations covered by medicaid in the united states. | medicaid infants are at high risk of severe respiratory syncytial virus (rsv) disease. the study objective was to estimate the cost-effectiveness of palivizumab in a medicaid population. | 2012 | 22435648 |
| respiratory syncytial virus potentiates abca3 mutation-induced loss of lung epithelial cell differentiation. | atp-binding cassette transporter a3 (abca3) is a lipid transporter active in lung alveolar epithelial type ii cells (atii) and is essential for their function as surfactant-producing cells. abca3 mutational defects cause respiratory distress in newborns and interstitial lung disease (ild) in children. the molecular pathomechanisms are largely unknown; however, viral infections may initiate or aggravate ilds. here, we investigated the impact of the clinically relevant abca3 mutations, p.q215k and ... | 2012 | 22434821 |
| identification of common biological pathways and drug targets across multiple respiratory viruses based on human host gene expression analysis. | pandemic and seasonal respiratory viruses are a major global health concern. given the genetic diversity of respiratory viruses and the emergence of drug resistant strains, the targeted disruption of human host-virus interactions is a potential therapeutic strategy for treating multi-viral infections. the availability of large-scale genomic datasets focused on host-pathogen interactions can be used to discover novel drug targets as well as potential opportunities for drug repositioning. | 2012 | 22432004 |
| 6 cmh2o continuous positive airway pressure versus conventional oxygen therapy in severe viral bronchiolitis: a randomized trial. | to compare the effects of nasal continuous positive airway pressure (ncpap) and conventional oxygen therapy on the clinical signs of respiratory distress and the respiratory muscle workload in acute viral bronchiolitis. | 2013 | 22431446 |
| epidemiology and seasonality of respiratory viral infections in hospitalized children in kuala lumpur, malaysia: a retrospective study of 27 years. | viral respiratory tract infections (rti) are relatively understudied in southeast asian tropical countries. in temperate countries, seasonal activity of respiratory viruses has been reported, particularly in association with temperature, while inconsistent correlation of respiratory viral activity with humidity and rain is found in tropical countries. a retrospective study was performed from 1982-2008 to investigate the viral etiology of children (≤ 5 years old) admitted with rti in a tertiary h ... | 2012 | 22429933 |
| viruses associated with pneumonia in adults. | viral pneumonia, which is typically associated with disease in childhood, is increasingly recognized as causing problems in adults. certain viruses, such as influenza virus, can attack fully immunocompetent adults, but many viruses take advantage of more-vulnerable patients. the latter include patients receiving immunosuppressive therapy and elderly subjects, particularly those residing in long-term care facilities. the range of viruses producing pneumonia in adults includes common agents, such ... | 2012 | 22423119 |
| [study on the effect of realgar nanoparticles on reducing the respiratory syncytial virus type a (rsv-a) replication in vitro]. | this study was to establish a model to explore anti- rsv effect of different administration method of chinese medicine realgar on respiratory syncytial virus type a (rsv-a) replication in hep-2 cells. using high-energy ball milling with distilled water to prepare realgar nanoparticles,the concentration of nanometer realgar was tested by molybdenum blue staining method and the size of realgar nanoparticles was tested on nano series. cell culture with ribavirin as a positive control was applied to ... | 2012 | 22416349 |
| mortality and morbidity of nosocomial respiratory syncytial virus (rsv) infection in ventilated children--a ten year perspective. | respiratory syncytial virus (rsv) is one of the most common pathogens involved in nosocomial infection in children. the aim of the study was to determine the impact of nosocomial rsv infection on mortality and pediatric intensive care unit (picu) morbidity of ventilated children. | 2012 | 22415435 |
| differential expression of cytokines in response to respiratory syncytial virus infection of calves with high or low circulating 25-hydroxyvitamin d3. | deficiency of serum levels of 25-hydroxyvitamin d(3) has been related to increased risk of lower respiratory tract infections in children. respiratory syncytial virus (rsv) is a leading cause of low respiratory tract infections in infants and young children. the neonatal calf model of rsv infection shares many features in common with rsv infection in infants and children. in the present study, we hypothesized that calves with low circulating levels of 25-hydroxyvitamin d(3) (25(oh)d(3)) would be ... | 2012 | 22412984 |
| in vitro modeling of respiratory syncytial virus infection of pediatric bronchial epithelium, the primary target of infection in vivo. | respiratory syncytial virus (rsv) is the major viral cause of severe pulmonary disease in young infants worldwide. however, the mechanisms by which rsv causes disease in humans remain poorly understood. to help bridge this gap, we developed an ex vivo/in vitro model of rsv infection based on well-differentiated primary pediatric bronchial epithelial cells (wd-pbecs), the primary targets of rsv infection in vivo. our rsv/wd-pbec model demonstrated remarkable similarities to hallmarks of rsv infec ... | 2012 | 22411804 |
| selection and characterization of human respiratory syncytial virus escape mutants resistant to a polyclonal antiserum raised against the f protein. | a human respiratory syncytial virus (hrsv) neutralization escape mutant was obtained after 56 serial passages in the presence of a polyclonal antiserum raised against the f protein. nucleotide sequence analysis of this escape mutant virus revealed two amino acid substitutions: asn268ile and val533met. when this virus was allowed to grow in the absence of the anti-f polyclonal serum, only the mutation asn268ile was stably maintained. both the double and single escape mutant viruses lost reactivit ... | 2012 | 22411099 |
| immunology of bovine respiratory syncytial virus infection of cattle. | bovine respiratory syncytial virus (brsv) is a respiratory pathogen of cattle that causes severe disease in calves alone and as one of several viruses and bacteria that cause bovine respiratory disease complex. like human rsv this virus modulates the immune response to avoid stimulation of a vibrant cd8+ t cytotoxic cell response and instead promotes a th2 response. the th2 skew sometimes results in the production of ige antibodies and depresses production of the th1 cytokine interferon γ. innat ... | 2012 | 22410266 |
| characterization of neutralizing affinity-matured human respiratory syncytial virus f binding antibodies in the sub-picomolar affinity range. | in the human adaptation and optimization of a mouse anti-human respiratory syncytial virus neutralizing antibody, affinity assessment was crucial to distinguish among potential candidates and to evaluate whether this correlated with function in vitro and in vivo. this affinity assessment was complicated by the trimeric nature of the antigen target, respiratory syncytial virus f (rsv-f) glycoprotein. in the initial affinity screen, surface plasmon resonance was used to determine the intrinsic bin ... | 2012 | 22407977 |
| evaluation of viral load in infants hospitalized with bronchiolitis caused by respiratory syncytial virus. | the relationship between viral load, disease severity and antiviral immune activation in infants suffering from respiratory syncytial virus (rsv)-associated bronchiolitis has not been well identified. the main objective of this study was to determine the existence of a correlation between rsv load and disease severity and also between different clinical markers and mrna levels of the interferon stimulated gene (isg)56 in infants hospitalized for bronchiolitis. we also evaluated whether viral loa ... | 2012 | 22406873 |
| mortality caused by influenza and respiratory syncytial virus by age group in england and wales 1999-2010. | the mortality burden caused by influenza cannot be quantified directly from death certificates because of under-recording; therefore, the estimated number of influenza deaths has to be obtained through statistical modelling. | 2013 | 22405488 |
| a preliminary study of pneumonia etiology among hospitalized children in kenya. | pneumonia is the leading cause of childhood death in the developing world. higher-quality etiological data are required to reduce this mortality burden. | 2012 | 22403235 |
| lower respiratory infections among hospitalized children in new caledonia: a pilot study for the pneumonia etiology research for child health project. | we conducted a prospective pilot study over a 1-year period in new caledonia in preparation for the pneumonia research for child health (perch) project. the pathogens associated with hospitalized lower respiratory infections in children were identified through the use of culture of induced sputum and blood, urinary antigen detection, polymerase chain reaction (pcr) on respiratory specimens, and serology on paired sera. respiratory viruses were detected on respiratory specimens by immunofluoresce ... | 2012 | 22403234 |
| prevalence and genetic characterization of respiratory syncytial virus (rsv) in hospitalized children in korea. | human respiratory syncytial virus (hrsv) is the most common respiratory pathogen among infants and young children. to investigate the prevalence and genetic characteristics of hrsvs circulating in south korea, we analyzed medical records of patients and performed molecular analysis of the g-protein gene of viruses detected from nasopharyngeal aspirates (npa) of admitted patients at the pediatrics department of chungbuk national university hospital from april 2008 to april 2010. epidemiological d ... | 2012 | 22402914 |
| plasticity and virus specificity of the airway epithelial cell immune response during respiratory virus infection. | airway epithelial cells (aecs) provide the first line of defense in the respiratory tract and are the main target of respiratory viruses. here, using oligonucleotide and protein arrays, we analyze the infection of primary polarized human aec cultures with influenza virus and respiratory syncytial virus (rsv), and we show that the immune response of aecs is quantitatively and qualitatively virus specific. differentially expressed genes (degs) specifically induced by influenza virus and not by rsv ... | 2012 | 22398282 |
| prophylaxis and treatment of respiratory syncytial virus in adult immunocompromised patients. | to review the literature regarding current strategies and strategies under active development for the prevention and treatment of respiratory syncytial virus (rsv) infections in immunocompromised adults. | 2012 | 22395247 |
| il-10 regulates viral lung immunopathology during acute respiratory syncytial virus infection in mice. | interleukin (il-) 10 is a pleiotropic cytokine with broad immunosuppressive functions, particularly at mucosal sites such as the intestine and lung. here we demonstrate that infection of balb/c mice with respiratory syncytial virus (rsv) induced il-10 production by cd4(+) and cd8(+) t cells in the airways at later time points (e.g. day 8); a proportion of these cells also co-produced ifn-γ. furthermore, rsv infection of il-10(-/-) mice resulted in more severe disease with enhanced weight loss, d ... | 2012 | 22393401 |
| resveratrol inhibits respiratory syncytial virus-induced il-6 production, decreases viral replication, and downregulates trif expression in airway epithelial cells. | respiratory syncytial virus (rsv) is the most common pathogen responsible for lower respiratory diseases in children. so far, there is no effective treatment or preventative vaccine available for rsv infection, although ribavirin and dexamethasone are commonly prescribed. resveratrol has been shown to inhibit the replication of several other viruses, thus the effect of resveratrol on rsv-induced inflammatory mediators in 9hteo cell cultures was evaluated, and possible mechanisms of action were e ... | 2012 | 22391746 |
| [clinical aspects and treatment of respiratory syncytial virus infection in infants in the first 6 months of life]. | respiratory viral infection are one of the most frequent problem in infant and the younger children. respiratory syncytial virus infects nearly all children (75-100%) in the first 36 months of life. | 2011 | 22390047 |
| emergency room management of acute bronchiolitis: a randomized trial of nebulized epinephrine. | acute bronchiolitis is a common, potentially life-threatening condition with few therapeutic options. in the present randomized study, we compared the clinical efficacies of nebulized epinephrine and salbutamol in the emergency room management of acute bronchiolitis. primary outcome measures were improvement in mean respiratory rate, mean oxygen saturation value and severity score. secondary outcome measures were length of hospital stay, hospitalization and relapse rates. a total of 75 patients ... | 2011 | 22389988 |
| etiology and clinical characterization of respiratory virus infections in adult patients attending an emergency department in beijing. | acute respiratory tract infections (artis) represent a serious global health burden. to date, few reports have addressed the prevalence of respiratory viruses (rvs) in adults with artis attending an emergency department (ed). therefore, the potential impact of respiratory virus infections on such patients remains unknown. | 2012 | 22389685 |
| structure of the human metapneumovirus fusion protein with neutralizing antibody identifies a pneumovirus antigenic site. | human metapneumovirus and respiratory syncytial virus cause lower respiratory tract infections. the virus fusion (f) glycoprotein promotes membrane fusion by refolding from a metastable pre-fusion to a stable post-fusion conformation. f is also a major target of the neutralizing antibody response. here we show that a potently neutralizing anti-human metapneumovirus antibody (ds7) binds a structurally invariant domain of f, revealing a new epitope that could be targeted in vaccine development. | 2012 | 22388735 |
| in vivo modulation of the innate response to pneumovirus by type-i and -iii interferon-induced bos taurus mx1. | the respiratory syncytial virus (rsv) is a major pathogen of the human species. this pneumovirus is a prominent cause of airway morbidity in children and maintains an excessive hospitalization rate despite decades of research. as involvement of a genetic vulnerability is a possibility supported by recent data, we addressed the question of whether the mx gene products, the typical target of which consists in single-stranded negative-polarity rna viruses, could alter the course of pneumovirus-asso ... | 2012 | 22385204 |
| respiratory viral infections during the first 28 days after transplantation in pediatric hematopoietic stem cell transplant recipients. | respiratory viruses (rvs) are a known cause of morbidity and mortality after hematopoietic stem cell transplantation (hsct). in this retrospective study, we focused on the first 28 d after transplantation in pediatric hsct recipients and showed that a multiplex pcr assay significantly increased rv detection compared with a viral culture method. among 176 pediatric hsct recipients, 84 with respiratory symptoms within one yr after hsct were tested by viral culture or multiplex pcr. within 28 d aft ... | 2012 | 22385011 |
| dual role of respiratory syncytial virus glycoprotein fragment as a mucosal immunogen and chemotactic adjuvant. | respiratory syncytial virus (rsv) is a major cause of severe lower respiratory tract disease in infancy and early childhood. despite its importance as a pathogen, there is no licensed vaccine to prevent rsv infection. the g glycoprotein of rsv, a major attachment protein, is a potentially important target for protective antiviral immune responses and has been shown to exhibit chemotactic activity through cx3c mimicry. here, we show that sublingual or intranasal immunization of a purified g prote ... | 2012 | 22384186 |
| incidence of respiratory virus-associated pneumonia in urban poor young children of dhaka, bangladesh, 2009-2011. | pneumonia is the leading cause of childhood death in bangladesh. we conducted a longitudinal study to estimate the incidence of virus-associated pneumonia in children aged <2 years in a low-income urban community in dhaka, bangladesh. | 2012 | 22384139 |
| respiratory syncytial virus ns1 protein colocalizes with mitochondrial antiviral signaling protein mavs following infection. | respiratory syncytial virus (rsv) nonstructural protein 1(ns1) attenuates type-i interferon (ifn) production during rsv infection; however the precise role of rsv ns1 protein in orchestrating the early host-virus interaction during infection is poorly understood. since ns1 constitutes the first rsv gene transcribed and the production of ifn depends upon rlr (rig-i-like receptor) signaling, we reasoned that ns1 may interfere with this signaling. herein, we report that ns1 is localized to mitochon ... | 2012 | 22383950 |
| evaluation of measles vaccine virus as a vector to deliver respiratory syncytial virus fusion protein or epstein-barr virus glycoprotein gp350. | live attenuated recombinant measles vaccine virus (mv) edmonston-zagreb (ez) strain was evaluated as a viral vector to express the ectodomains of fusion protein of respiratory syncytial virus (rsv f) or glycoprotein 350 of epstein-barr virus (ebv gp350) as candidate vaccines for prophylaxis of rsv and ebv. the glycoprotein gene was inserted at the 1(st) or the 3(rd) position of the measles virus genome and the recombinant viruses were generated. insertion of the foreign gene at the 3(rd) positio ... | 2012 | 22383906 |
| molecular epidemiology of the attachment glycoprotein (g) gene in respiratory syncytial virus in children with acute respiratory infection in japan in 2009/2010. | this study performed a detailed genetic analysis of the glycoprotein (g) gene of respiratory syncytial virus (rsv) detected in 50 japanese children with acute respiratory infection (ari) in the 2009/2010 season. a phylogenetic tree constructed by the neighbour-joining method showed that 34 and 16 of the rsv strains could be classified into subgroups a and b, respectively. strains belonging to subgroups a and b were further subdivided into ga2 and ba, respectively. the nucleotide and deduced amin ... | 2012 | 22383445 |
| a new biflavonoid with antiviral activity from the roots of wikstroemia indica. | a new biflavonoid, 4'-methoxydaphnodorin e, was isolated from the antiviral fraction of wikstroemia indica against respiratory syncytial virus (rsv). its structure was determined on the basis of extensive spectroscopic data including hr-esi-ms and 2d nmr. the biflavonoid was tested for its in vitro anti-rsv activity with cytopathic effect (cpe) reduction assay, and displayed potent effect with 50% inhibitory concentration (ic₅₀) value of 2.8 μm and selective index (si) value of 5.4. | 2012 | 22375879 |
| evaluation of interleukin 28b single nucleotide polymorphisms in infants suffering from bronchiolitis. | the genetic diversity of the host is believed to be the key of the diversity in the clinical presentation of bronchiolitis. the aim of this study was to determine whether the known rs12979860 and rs8099917 single nucleotide polymorphisms (snps) in interleukin (il)28b region, influence clinical features and natural history of bronchiolitis. both snps showed no significant association with the risk of hospitalization for respiratory syncytial virus (rsv), viral load, disease severity, and other cl ... | 2012 | 22374338 |
| the role of viruses in the pathogenesis of peritonsillar abscess. | peritonsillar abscess (pta) is the most frequent complication of acute tonsillitis and a prevalent cause for acute admission to otorhinolaryngology departments. our aim was to examine the role of viruses in the pathogenesis of pta, as this has not previously been considered. we examined both palatine tonsils from 25 patients undergoing acute tonsillectomy for pta, using pcr-based assays for herpes simplex virus-1 and -2 (hsv-1 and -2), adenovirus, epstein-barr virus (ebv), influenza a and b, and ... | 2012 | 22373896 |
| new respiratory viral infections. | the first era in the discoveries of respiratory viruses occured between 1933 and 1965 when influenza virus, enteroviruses, adenovirus, respiratory syncytial virus, rhinovirus, parainfluenza virus and coronavirus (cov) were found by virus culture. in the 1990s, the development of high throughput viral detection and diagnostics instruments increased diagnostic sensitivity and enabled the search for new viruses. this article briefly reviews the clinical significance of newly discovered respiratory ... | 2012 | 22366993 |
| inflammatory responses to respiratory syncytial virus (rsv) infection and the development of immunomodulatory pharmacotherapeutics. | respiratory syncytial virus (rsv; family paramyxoviridae, genus pneumovirus) is a major respiratory pathogen of infants and children and an emerging pathogen of the elderly. current management of rsv disease includes monoclonal antibody prophylaxis for infants identified as high risk and supportive care for those with active infection; there is no vaccine, although several are under study. in this manuscript, we review published findings from human autopsy studies, as well as experiments that fo ... | 2012 | 22360479 |
| a microcarrier-based cell culture process for the production of a bovine respiratory syncytial virus vaccine. | veterinary viral vaccines generally comprise either attenuated or chemically inactivated viruses which have been propagated on mammalian cell substrates or specific pathogen free (spf) eggs. new generation vaccines include chemically inactivated virally-infected whole cell vaccines. the nm57 cell line is a bovine nasal turbinate persistently infected (non-lytic infection) with a strain of the respiratory syncytial virus (rsv). the potential of microcarrier technology for the cultivation in biore ... | 1999 | 22359062 |
| respiratory syncytial virus, infants and intensive therapy. | the aims of this study were to determine the presence of respiratory syncytial virus (rsv) and to assess the clinical features of the disease in infants with acute low respiratory tract infection hospitalized at pediatric intensive care units (picu) of two university teaching hospitals in são paulo state, brazil. nasopharyngeal secretions were tested for the rsv by the polymerase chain reaction. positive and negative groups for the virus were compared in terms of evolution under intensive care ( ... | 2017 | 22358363 |
| a challenge to appropriate antibiotic use in children with respiratory infections: a 5-year single-institution experience. | we have studied the rate of emergence of antibiotic-resistant streptococcus pneumoniae (s. pneumoniae) and haemophilus influenzae (h. influenzae) and the subsequent antibiotic use in host patients of those isolates at the department of pediatrics, soma general hospital, fukushima. moreover, we carried out several studies investigating the risks and benefits of antibiotic-free treatment for children with respiratory infections. in this report, we summarize our research and suggest better treatmen ... | 2011 | 22353649 |
| [veno-arterial ecmo as bridge to recovery. cardiogenic shock and suspected myocarditis in a 37-year-old patient]. | we report a case of a 37-year-old patient presenting with fulminant cardiogenic shock, almost noncontractile ventricles, followed by electromechanical dissociation. during performance of cardiopulmonary resuscitation, a veno-arterial extracorporeal membrane oxygenation device (va ecmo) was implanted, which became necessary for 13 days. subsequently, a total arrest of ventricular function was observed and prominent multiple organ failure emerged. a rapid test for respiratory syncytial virus was p ... | 2012 | 22349535 |