Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| inheritance of tolerance to rice tungro bacilliform virus (rtbv) in rice (oryza sativa l.). | from a large number of rice varieties tested, no variety was identified as resistant to tungro bacilliform virus (rtbv). only in utri merah was the rtbv multiplication restrictive, whereas other varieties such as kataribhog and pankhari 203 were identified as tolerant. these varieties were crossed with a susceptible variety. tn1, to study the inheritance of restrictive multiplication and tolerance to rtbv. after 3 weeks of inoculation with rtbv, f1; f2, and f3 progenies were assessed by enzyme-l ... | 1990 | 24221010 |
| mitochondrial dna variation in long-term tissue cultured rice lines. | the effects of long-term tissue culture on mitochondrial dnas were examined using rice (oryza sativa) cell suspension cultures. mitochondrial dnas were isolated from p. i. 353705 (an indica subspecies of rice similar to 'asam 5'), its anther-culture-derived line bl2 (an 8-year-old cell suspension culture), and five other cell lines (a1, a7, a11, a13, and a23), also derived from bl2 and independently selected for resistance to the lysine analog, s-(2-amino)-ethyl-l-cysteine. mitochondrial dnas of ... | 1990 | 24220814 |
| identification of mitogen-activated protein kinase kinase gene family and mkk-mapk interaction network in maize. | plant mitogen-activated protein kinases (mapk) are involved in important processes, including stress signaling and development. mapk kinases (mapkk, mkk) have been investigated in several plant species including arabidopsis thaliana, oryza sativa, populus trichocarpa, and brachypodium distachyon. in the present study, nine putative maize mkk genes have been identified. analysis of the conserved protein motifs, exon-intron junctions and intron phase has revealed high levels of conservation within ... | 2013 | 24220337 |
| transcriptomes of isolated oryza sativa gametes characterized by deep sequencing: evidence for distinct sex-dependent chromatin and epigenetic states before fertilization. | the formation of a zygote by the fusion of egg and sperm involves the two gametic transcriptomes. in flowering plants, the embryo sac embedded within the ovule contains the egg cell, whereas the pollen grain contains two sperm cells inside a supporting vegetative cell. the difficulties of collecting isolated gametes and consequent low recovery of rna have restricted in-depth analysis of gametic transcriptomes in flowering plants. we isolated living egg cells, sperm cells and pollen vegetative ce ... | 2013 | 24215296 |
| interaction between rice stripe virus disease-specific protein and host psbp enhances virus symptoms. | rice stripe virus (rsv) causes severe diseases in oryza sativa (rice) in many eastern asian countries. disease-specific protein (sp) of rsv is a non-structural protein and its accumulation level in rice plant was shown to determine the severity of rsv symptoms. here, we present evidence that expression of rsv sp alone in rice or nicotiana benthamiana did not produce visible symptoms. expression of sp in these two plants, however, enhanced rsv- or potato virus x (pvx)-induced symptoms. through ye ... | 2014 | 24214893 |
| plant regeneration from haploid cell suspension-derived protoplasts of mediterranean rice (oryza sativa l. cv. miara). | more than 750 plants were regenerated from protoplasts isolated from microspore callus-derived cell suspensions of the mediterranean japonica rice miara, using a nurse-feeder technique and n6-based culture medium. the mean plating efficiency and the mean regeneration ability of the protocalluses were 0.5% and 49% respectively. flow cytometric evaluation of the dna contents of 7 month old-cell and protoplast suspensions showed that they were still haploid. contrastingly, the dna contents of leaf ... | 1992 | 24213364 |
| regeneration of herbicide resistant transgenic rice plants following microprojectile-mediated transformation of suspension culture cells. | suspension cells of oryza sativa l. (rice) were transformed, by microprojectile bombardment, with plasmids carrying the coding region of the streptomyces hygroscopicus phosphinothricin acetyl transferase (pat) gene (bar) under the control of either the 5' region of the rice actin 1 gene (act1) or the cauliflower mosaic virus (camv) 35s promoter. subsequently regenerated plants display detectable pat activity and are resistant to basta(tm), a phosphinothricin (ppt)-based herbicide. dna gel blot a ... | 1992 | 24213292 |
| simple dehydration treatment promotes plantlet regeneration of rice (oryza sativa l.) callus. | to increase plantlet regeneration frequency, rice callus was dehydrated in a petri dish with a single layer of filter paper prior to transfer to the regeneration medium. with a 24 h dehydration treatment, the regeneration frequency was increased to 47 %, while the regeneration frequency of the untreated control was less than 5 %. this relatively simple method provides an alternative method for improving the regeneration frequency of rice callus. | 1992 | 24213284 |
| restriction fragment length polymorphism analysis of polymerase chain reaction products amplified from mapped loci of rice (oryza sativa l.) genomic dna. | thirty mapped indica rice genomic (rg) clones were partially sequenced from each end. from such sequence data, pairs of oligonucleotides were synthesized to act as primers for polymerase chain reaction (pcr) amplification of the corresponding loci in crude total dna preparations. the pcr products from dna of indica varieties were of the sizes expected from the sizes of the corresponding rg clones. however, size polymorphisms were seen between pcr products from indica and japonica varieties, and ... | 1991 | 24213267 |
| chromosomal localization and genomic organization of α-amylase genes in rice (oryza sativa l.). | genes for α-amylase, alcohol dehydrogenase, andem, an aba-regulated gene expressed late in embryogenesis, were localized on rice chromosomes by the analysis of primary trisomies. the validity of the mapping approach was confirmed usingadh-1 as a control. theadh-1 gene has previously been assigned to chromosome 11 using conventional techniques. in this study we confirm this assignment and report an additional locus for alcohol dehydrogenase (adh-2) on chromosome 9. the α-amylase genes were locate ... | 1991 | 24213266 |
| selection and characterization of a rice mutant resistant to 5-methyltryptophan. | a rice plant resistant to 5-methyltryptophan (5mt) was selected from mutagenized m3 seeds (oryza sativa l. var. sasanishiki) originating from panicles treated with ethylene imine (0.2%) 2 h after flowering. when germinated on 5mt-containing medium, the seeds (m4) from selfed plants segregated with a 3 resistant:1 sensitive ratio, indicating that the plant was heterozygous for a resistance gene and that the resistance was dominant. the resistance was also expressed in callus derived from seeds. a ... | 1991 | 24213253 |
| plant regeneration from indica rice (oryza sativa l.) protoplasts. | rice (oryza sativa l.) plants of the indica cultivar ir54 were regenerated from protoplasts. conditions were developed for isolating and purifying protoplasts from suspension cultures with protoplast yields ranging from 1·10(6) to 15·10(6) viable protoplasts/1 g fresh weight. protoplast viability after purification was generally over 90%. protoplasts were cultured in a slightly modified kao medium in a petri plate by placing them onto a millipore filter positioned on top of a feeder (nurse) cult ... | 1989 | 24212899 |
| a simple sample preparation approach based on hydrophilic solid-phase extraction coupled with liquid chromatography-tandem mass spectrometry for determination of endogenous cytokinins. | cytokinins (cks), a vital family of phytohormones, play important roles in the regulation of shoot and root development. however, the quantification of cks in plant samples is frequently affected by the complex plant matrix. in the current study, we developed a simple, rapid and efficient hydrophilic interaction chromatography-solid phase extraction (hilic-spe) method for cks purification. cks were extracted by acetonitrile (acn) followed by hilic-spe (silica as sorbents) purification. the extra ... | 2013 | 24212141 |
| identification and characterization of granule bound starch synthase i (gbssi) gene of tartary buckwheat (fagopyrum tataricum gaertn.). | tartary buckwheat (fagopyrum tataricum gaertn.) is increasingly considered as an important functional food material because of its rich nutraceutical compounds. reserve starch is the major component of tartary buckwheat seed. however, the gene sequences and the molecular mechanism of tartary buckwheat starch synthesis are unknown so far. in this study, the complete genomic sequence and full-size cdna coding tartary buckwheat granule-bound starch synthase i (ftgbssi), which is responsible for amy ... | 2014 | 24211386 |
| computational evaluation on the binding affinity of non-specific lipid-transfer protein-2 with fatty acids. | a computational study was carried out to identify the structural determinant controlling the affinity, specificity and binding strength of several saturated and unsaturated fatty acids with oryza sativa (indica group) nonspecific lipid transfer protein (nsltp2). association between the number, position and conformation of hydrophobic patches and lipid binding properties of the protein was evidenced by docking analysis. binding affinity is influenced by the number of carbon atoms, location of dou ... | 2013 | 24209919 |
| laterally resolved speciation of arsenic in roots of wheat and rice using fluorescence-xanes imaging. | • accumulation of arsenic (as) within plant tissues represents a human health risk, but there remains much to learn regarding the speciation of as within plants. • we developed synchrotron-based fluorescence-x-ray absorption near-edge spectroscopy (fluorescence-xanes) imaging in hydrated and fresh plant tissues to provide laterally resolved data on the in situ speciation of as in roots of wheat (triticum aestivum) and rice (oryza sativa) exposed to 2 μm as(v) or as(iii). • when exposed to as(v), ... | 2014 | 24206613 |
| genetic recombination is associated with intrinsic disorder in plant proteomes. | intrinsically disordered proteins, found in all living organisms, are essential for basic cellular functions and complement the function of ordered proteins. it has been shown that protein disorder is linked to the g + c content of the genome. furthermore, recent investigations have suggested that the evolutionary dynamics of the plant nucleus adds disordered segments to open reading frames alike, and these segments are not necessarily conserved among orthologous genes. | 2013 | 24206529 |
| rice sucrose transporter1 (ossut1) up-regulation in xylem parenchyma is caused by aphid feeding on rice leaf blade vascular bundles. | the role of the sucrose transporter ossut1 in assimilate retrieval via the xylem, as a result of damage to and leakage from punctured phloem was examined after rusty plum aphid (hysteroneura setariae, thomas) infestation on leaves from 3-week-old rice (oryza sativa l. cv nipponbare) plants. leaves were examined over a 1- to 10-day infestation time course, using a combination of gene expression and β-glucuronidase (gus) reporter gene analyses. qpcr and western blot analyses revealed differential ... | 2014 | 24206428 |
| genome-wide computational prediction and analysis of core promoter elements across plant monocots and dicots. | transcription initiation, essential to gene expression regulation, involves recruitment of basal transcription factors to the core promoter elements (cpes). the distribution of currently known cpes across plant genomes is largely unknown. this is the first large scale genome-wide report on the computational prediction of cpes across eight plant genomes to help better understand the transcription initiation complex assembly. the distribution of thirteen known cpes across four monocots (brachypodi ... | 2013 | 24205361 |
| relationship between disease resistance and rice oxalate oxidases in transgenic rice. | differential expression of rice oxalate oxidase genes (osoxo1-4) in rice leaves (oryza sativa l.) in response to biotic stress was assayed using rt-pcr. osoxo4 was induced transiently at 12 h in plants inoculated with the pathogens of bacterial blight and that of the wounding control. inoculation with the rice blast pathogen induced osoxo2 expression compared to the mock spray control. overexpressing osoxo1 or osoxo4 in rice resulted in elevated transcript levels of the respective transgene as w ... | 2013 | 24205207 |
| transfer of bacterial blight and blast resistance from the tetraploid wild rice oryza minuta to cultivated rice, oryza sativa. | oryza minuta j. s. presl ex c. b. presl is a tetraploid wild rice with resistance to several insects and diseases, including blast (caused by pyricularia grisea) and bacterial blight (caused by xanthomonas oryzae pv. oryzae). to transfer resistance from the wild species into the genome of cultivated rice (oryza sativa l.), backcross progeny (bc1, bc2, and bc3) were produced from interspecific hybrids of o. sativa cv 'ir31917-45-3-2' (2n=24, aa genome) and o. minuta acc. 101141 (2n=48, bbcc genom ... | 1992 | 24203194 |
| efficient fertile plant regeneration from protoplasts of the indica rice breeding line ir72 (oryza sativa l.). | protoplasts were isolated from embryogenic cell suspensions obtained from mature seed derived embryogenic callus of the advanced indica type rice breeding line ir72 available from irri (international rice research institute), manila. culture of protoplasts with the agarose bead type method without nurse culture led to sustained proliferation of protoplast derived clones. a simple culture protocol was developed which stimulated embryogenic development. germination of somatic embryos has so far pr ... | 1992 | 24203129 |
| characterization of a rice (oryza sativa l.) mutant deficient in the heme domain of nitrate reductase. | biochemical and genetical characterization of a rice nitrate reductase (nr)-deficient mutant, m819, which had been isolated as a chlorate-resistant mutant, was carried out. in m819, leaf nadh-nr activity was found to be about 10% of that of the wild-type cv 'norin 8', while nadph-nr activity was higher than that in the wild-type; fmnh2-nr and mv-nr activities were also 10% of those of the wild type; bpb-nr activity was higher than that of the wild type; and xanthine dehydrogenase activity was re ... | 1992 | 24203021 |
| genome specificity of rdna spacer fragments from oryza sativa l. | the intergenic spacer derived from a cloned rdna unit from a cultivated rice was dissected into several subclones, which were used as probes to analyze sequence homologies between rdna spacers from wild rice belonging to genome types aa, bb, cc, ee, ff, bbcc, and ccdd. this analysis allowed us to detect several regions with different degrees of homology. a series of 250-260 bp repeats is located in the central part of the aa spacer. this sequence cross-hybridizes with the bb, cc, bbcc, and ff ge ... | 1992 | 24202765 |
| genetic diversity and differentiation of indica and japonica rice detected by rflp analysis. | genetic diversity and differentiation in indica and japonica groups of the cultivated rice (oryza sativa l.) were studied by assaying dna restriction fragment length polymorphisms of 12 indica and 14 japonica rice lines digested with three restriction endonucleases. a total of 49 probes were selected to represent the entire rflp map at intervals of 20-30 cm. it was shown that 95 of the 145 possible probe/enzyme combinations, involving 43 probes and all three enzymes, detected restriction fragmen ... | 1992 | 24202597 |
| mitochondrial genome structure of rice suspension culture from cytoplasmic male-sterile line (a-58cms): reappraisal of the master circle. | the mitochondrial dna (mtdna) from the cultured cells of a cytoplasmic male-sterile line (a-58cms) of rice (oryza sativa) was cloned and its physical map was constructed. there was structural alteration on the mitochondrial genome during the cell culture. detailed restriction analysis of cosmid clones having mtdna fragments suggested either that the master genome has a 100-kb duplication (the genome size becomes 450 kb) or that a master circle is not present in the genome (the net structural com ... | 1992 | 24202508 |
| diallel analysis of tiller number at different growth stages in rice (oryza sativa l.). | the genetics of tiller number at different development stages in rice was studied in a 6 x 6 diallel cross set involving six indica rice varieties in which a large difference of phenotypes existed. estimates of genetic parameters following hayman's method showed significant additive and nonadditive gene action, and the latter appeared to be solely due to dominance. high tillering ability was inherited as a partial dominant character conditioned by two or more blocks of genes, and var 'h1459' had ... | 1991 | 24202365 |
| structural homology between semidwarfism-related proteins and glutelin seed protein in rice (oryza sativa l.). | two semidwarfism-related proteins, srp-1 and srp-2, were detected as major spots in a long-culm rice cultivar, norin 29 and its semidwarf near-isogenic line, sc-tn1, respectively, by two-dimensional gel electrophoresis (2d-page). the testcross showed that srp-1 and srp-2 are controlled by codominant alleles, srp-1 and srp-2, respectively, at a single locus srp. this locus was considered to be closely linked with the semidwarfing gene locus sd-1. srp-1 and srp-2 were separated by 2d-page, electro ... | 1991 | 24202351 |
| genetic analysis of endosperm mutants in rice oryza sativa l. | sugary, shrunken, floury, white core, amylose extender and dull mutants induced in japonica varieties were used in this study. the results of an allelic analysis conducted in japonica background indicated that the two sugary mutants 82gf and em5 are allelic. the two amylose extender mutants 2064 and em16 are also allelic. the opaque mutant esd7-3(0) and floury mutants 2047, em17 and em28 are allelic as well and have the flo-1 gene. the three white core mutants em3, em24 and em66 were found to be ... | 1991 | 24202350 |
| field uniformity of the japonica rice region of taiwan as estimated by relative genetic contribution. | despite the concerns for genetic vulnerability that were raised in the 1970s, the field uniformity of the japonica rice (oryza sativa l.) region in taiwan has increased since 1980 with over 82% of the cultivated areas being covered by as few as three varieties and over half of this hectarage by a single variety. japanese plant introductions are the major ancestral contributors of genetic constituents for varieties released in taiwan. the main constitution of the genetic base present in the field ... | 1991 | 24202264 |
| production of n + 1 plants and tetrasomics by means of anther culture of trisomic plants in rice (oryza sativa l.). | eleven primary trisomics of rice, variety nipponbare, were subjected to anther culture. the 12th trisomic did not produce normal anthers. a total of 3,734 plants were obtained, which were examined morphologically at the seedling stage in the greenhouse. a number of plants appeared in the progenies of ten trisomics which had unique morphological features. the frequency of these variant types differed among different progenies. cytological observations revealed that 43 variant plants in the progen ... | 1991 | 24202251 |
| localization of glyoxylate-cycle marker enzymes in peroxisomes of senescent leaves and green cotyledons. | crude particulate homogenates from leaves of barley (hordeum vulgare l.), rice (oryza sativa l.), leaf-beet (beta vulgaris var. cicla l.) and pumpkin (cucurbita pepo l.) cotyledons were separated on sucrose density gradients. the peroxisomal fractions appeared at a buoyant density of 1.25 g·cm(-3) and contained most of the activities of catalase (ec 1.11.1.6), and hydroxypyruvate reductase (ec 1.1.1.81) on the gradients. in peroxisomal fractions from detached leaves and green cotyledons incubate ... | 1990 | 24202025 |
| greenhouse gas emissions from rice microcosms amended with a plant microbial fuel cell. | methane (ch4) release from wetlands is an important source of greenhouse gas emissions. gas exchange occurs mainly through the aerenchyma of plants, and production of greenhouse gases is heavily dependent on rhizosphere biogeochemical conditions (i.e. substrate availability and redox potential). it is hypothesized that by introducing a biocatalyzed anode electrode in the rhizosphere of wetland plants, a competition for carbon and electrons can be invoked between electrical current-generating bac ... | 2014 | 24201892 |
| fertile indica and japonica rice plants regenerated from protoplasts isolated from embryogenic haploid suspension cultures. | a system to regenerate fertile rice (oryza sativa l.) plants (both indica and japonica varieties) from protoplasts isolated from anther-derived embryogenic haploid suspension cultures has been established. green plants were regenerated from protoplast-derived cell clusters five months after suspension culture initiation. protoplast yields and subsequent growth of the protoplast-derived microcalli were enhanced by transferring suspension cells into aa medium (muller et al. 1978) three to four day ... | 1992 | 24201730 |
| membrane-associated binding sites for indoleacetic acid in the rice coleoptile. | as described previously, the sensitivity of rice (oryza sativa l.) coleoptiles to auxin is modulated by oxygen. under anoxia, coleoptile elongation is insensitive to exogenously applied indole-3-acetic acid (iaa), whereas its sensitivity increases in air in the presence of the exogenous stimulus. here we report the presence of two independent classes of membrane-bound iaa-binding sites in air-grown coleoptiles. their binding activity is strictly correlated with the system's sensitivity to iaa. w ... | 1989 | 24201521 |
| identification of specific proteins from seed embryos by two-dimensional gel electrophoresis for the discrimination between indica and japonica rice. | proteins extracted from seed embryos of 29 different cultivated rice (oryza sativa l.) and one wild rice (o. rufipogon griff.) were compared by two-dimensional gel electrophoresis analysis. among more than 300 protein spots on the gel we found some interesting variations in ten spots which were individually designated as proteins a-j. protein e was observed in all indica cultivars but was not found in those of the subspecies japonica. in contrast, protein f was only detected in japonica cultivar ... | 1992 | 24201498 |
| segregation distortion via male gametes in hybrids between indica and japonica or wide-compatibility varieties of rice (oryza sativa l). | one or two marker genes on each of chromosomes 3, 4, 6, 7, 8, 11 and 12 of the 12 rice chromosomes were tested for segregation distortion in indica-japonica hybrids. marker genes on chromosomes 3, 7, 8, 11 and 12 showed clear segregation distortion. this distortion was not related to the proportion of normal pollen. the germinability of the pollen was less than 10% in the hybrids, although 45-55% of the pollen grains appeared to be morphologically normal. the frequent occurrence of segregation d ... | 1992 | 24201479 |
| rflp analysis of rice (oryza sativa l.) introgression lines. | fifty-two introgression lines (bc2f8) from crosses between two oryza sativa parents and five accessions of o. officinalis were analyzed for the introgression of o. officinalis chromosome segments. dna from the parents and introgression lines was analyzed with 177 rflp markers located at approximately 10-cm intervals over the rice chromosomes. most probe/enzyme combinations detected rflps between the parents. of the 174 informative markers, 28 identified putative o. officinalis introgressed chrom ... | 1992 | 24201348 |
| draft genome sequence of the plant pathogen dickeya zeae dz2q, isolated from rice in italy. | dickeya zeae is an emerging rice (oryza sativa) pathogen causing bacterial foot rot. related pathogens affect maize (zea mays) and potato (solanum tuberosum) and a variety of important ornamental and floral plants. here, we present the draft genome sequence of d. zeae dz2q, an isolate obtained from rice grown in italy. | 2013 | 24201194 |
| alignment of molecular and classical linkage maps of rice, oryza sativa. | application of genetic linkage maps in plant genetics and breeding can be greatly facilitated by integrating the available classical and molecular genetic linkage maps. in rice, oryza sativa l., the classical linkage map includes about 300 genes which correspond to various important morphological, physiological, biochemical and agronomic characteristics. the molecular maps consist of more than 500 dna markers which cover most of the genome within relatively short intervals. little effort has bee ... | 1993 | 24197352 |
| metabolic factors capable of inducing agrobacterium vir gene expression are present in rice (oryza sativa l.). | the effects of exudates and extracts from suspension cultures or various parts of rice (oryza sativa l.) plants on induction of vir (virulence) gene expression in agrobacterium tumefaciens were examined. only leaf extracts from panicle-differentiating plants to flowering plants were able to strongly induce activation and expression of vir genes. this induction was similar to that observed with 2 μm acetosyringone (as), yet there was no synergy between as and rice extracts. responses to vir-induc ... | 1993 | 24196854 |
| a major photoperiod-sensitivity gene tagged with rflp and isozyme markers in rice. | photoperiod-sensitive rice (oryza sativa l.) cultivars are widely grown in rainfed lowland areas with unfavorable water regimes. a molecular marker for the trait would be useful in genetic and physiological studies and in developing improved photoperiod-sensitive cultivars. previous genetic studies identified a major gene for photoperiod sensitivity on chromosome 6. we have tested an isozyme marker and several rflp probes mapping to chromosome 6 in an attempt to identify marker(s) tightly linked ... | 1993 | 24195926 |
| mitochondrial enzymes in aerobically and anaerobically germinated seedlings of echinochloa and rice. | activities of tricarboxylic acid (tca) cycle enzymes in seedlings of barnyard grass (echinochloa phyllopogon (stapf.) koss) and rice (oryza sativa l.) germinated under aerobic and anaerobic conditions were investigated. in e. phyllopogon, development of tca-cycle enzyme activities during 10 d of anoxia generally paralleled those in air, although at lower rates. after 5 d, e. phyllopogon seedlings germinating under n2 exhibited 50-80% of the activity of seedlings grown in air, except for 2-oxoglu ... | 1991 | 24194241 |
| fertile indica rice plants regenerated from protoplasts isolated from scutellar tissue of immature embryos. | we report on the regeneration of fertile indica rice (oryza sativa l.) plants from protoplasts isolated from scutellar tissue of immature embryos. the average yields of protoplasts after purification ranged from 2.8 × 10(5) to 3.5 × 10(5) protoplasts per fifty embryos. protoplasts developed rapidly to colonies when cultured in maltose containing medium using the nurse culture method. upto 146 or 39 visible colonies per 10(6) protoplasts were obtained for the varieties basmati 370 and ir43 respec ... | 1994 | 24194134 |
| increased doubled haploid plant regeneration from rice (oryza sativa l.) anthers cultured on colchicine-supplemented media. | plating rice anthers on a semisolid induction medium containing 250 or 500 mg/l colchicine for 24 or 48 h-incubations followed by transfer to colchicine-free medium and standard anther culture procedures resulted in overall 1.5- to 2.5- fold increases in doubled haploid green plant productions compared to control anther cultures. the addition of colchicine had no detrimental effects on the different anther culture efficiency parameters, but in some treatments led to significant enhancement of an ... | 1994 | 24194020 |
| peroxisomal enzyme activities in attached senescing leaves. | recently it has been demonstrated that detached leaves show glyoxysomal enzyme activities when incubated in darkness for several days. in this report glyoxylate-cycle enzymes have been detected in leaves of rice (oryza sativa l.) and wheat (triticum durum l.) from either naturally senescing or dark-treated plants. isolated peroxisomes of rice and wheat show isocitrate lyase (ec 4.1.3.1), malate synthase (ec 4.1.3.2) and β-oxidation activities. leaf peroxisomes from dark-induced senescing leaves ... | 1991 | 24193942 |
| molecular cloning and characterization of two complementary dnas encoding putative peroxidases from rice (oryza sativa l.) shoots. | pcr with oligonucleotide primers that corresponded to two highly homologous regions, in terms of amino acid sequence, of plant peroxidases was used to amplify a specific dna fragment from a mixture of rice (oryza sativa l.) cdnas. we then screened a cdna library prepared from mrnas of rice shoots utilizing the product of pcr as probe. two cdna clones, prxrpa and prxrpn, were isolated. they encode distinct isozymes of peroxidase. sequence analysis indicated that the clones encode mature proteins ... | 1994 | 24193902 |
| pre-germination genotypic screening using pcr amplification of half-seeds. | a simple and rapid pcr-based method has been developed for determining the genotype of seeds before germination. single half-seeds of rice (oryza sativa l.) and wheat (triticum aestivum l. em. thell.) were preincubated, without grinding, in an aqueous extraction buffer. the resulting supernatants were then used in polymerase chain reaction (pcr) with oligonucleotide primers corresponding to rice single-copy sequences or a wheat microsatellite repeat. pcr products of identical size were amplified ... | 1993 | 24193778 |
| variations in chloroplast dna from rice (oryza sativa): differences between deletions mediated by short direct-repeat sequences within a single species. | in a previous study, we compared chloroplast dnas (ctdnas) from four species oforyza and detected two independent deletions of dna fragments in the ctdnas (kanno and hirai 1992a). these deletions were genotype-specific variations. since short direct-repeat sequences were detected at the borders of both deletions, the deletions were apparently the result of intramolecular recombination mediated by these direct-repeat sequences. in the present study, we examined whether or not this type of variati ... | 1993 | 24193706 |
| genetic analysis of salinity tolerance in rice (oryza sativa l.). | the genetics of salinity tolerance in rice was investigated by a nine-parent complete diallel including reciprocals. test materials involved susceptible (ir28, ir29, and mi-48), moderately tolerant (ir4595-4-1-13, ir9884-54-3-1e-p1, and ir10206-29-2-1), and tolerant ("nona bokra", "pokkali", and sr26b) parents. twoweek-old seedlings were grown in a salinized (ec = 12 ds/m) culture solution for 19 days under controlled conditions in the irri phytotron. typical characteristics of salinity toleranc ... | 1993 | 24193479 |
| mutants having a low content of 16-kda allergenic protein in rice (oryza sativa l.). | rice mutants containing low levels of the 16-kda allergenic protein, which is the main allergen in the rice grain for patients of atopic dermatitis due to the intake of rice, were screened, and 4 independent mutant lines with small amounts of this protein were found by sds gel electrophoresis and immunoblot analysis. these mutants were grouped into two types. two mutant lines, 85kg-4 and 86rg-18, contained low levels of the 16k-da and 26-kda polypeptides and a high level of the 57-kda polypeptid ... | 1993 | 24193476 |
| characterization of protein synthesis by isolated rice mitochondria. | bacteria-free mitochondria were isolated from aseptically grown, etiolated and green seedlings of both cytoplasmic male-sterile (wa-type) and male-fertile rice (oryza sativa l.). protein synthesis in these isolated mitochondria was characterized by gel electrophoresis/fluorography and by the incorporation of [(35)s]-methionine into protein. in the presence of cycloheximide, a set of some 25 discrete polypeptides and an electrophoretically unresolved population were synthesized. this pattern of p ... | 1993 | 24193475 |
| comparative study on the chloroplast, mitochondrial and nuclear genome differentiation in two cultivated rice species, oryza sativa and o. glaberrima, by rflp analyses. | restriction fragment length polymorphisms of chloroplast (ct), mitochondrial (mt) and nuclear dna were investigated using eight cultivars of oryza sativa and two cultivars of o. glaberrima. relative variability in the nuclear and cytoplasmic genomes was estimated by a common measure, genetic distance. based on the average genetic distances among ten cultivars for each genome, the evolutionary variabilities of the mitochondrial and nuclear genomes were found to be almost the same, whereas the var ... | 1993 | 24193387 |
| hybrid rice (oryza sativa l.): identification and parentage determination by rapd fingerprinting. | dna from three families of rice plants selected in northern china (each comprising the male sterile, the restorer, the hybrid f1 and the maintainer lines) has been extracted and amplified by pcr with different random dna primers (rapd analysis). then, dna has been analysed by agarose gel electrophoresis and dna bands scored as present or absent. the generated matrices are reproducible and amenable for identification of each single plant line. thus, rapd fingerprinting of the inbred parental line ... | 1994 | 24192876 |
| differential expression of cpks and cytosolic ca2+ variation in resistant and susceptible apple cultivars (malus x domestica) in response to the pathogen erwinia amylovora and mechanical wounding. | plant calcium (ca2+) signals are involved in a wide array of intracellular signalling pathways following pathogen invasion. ca2+-binding sensory proteins such as ca2+-dependent protein kinases (cpks) have been predicted to mediate signalling following ca2+ influx after pathogen infection. however, to date this prediction has remained elusive. | 2013 | 24192013 |
| the cento satellite confers translational and rotational phasing on cenh3 nucleosomes in rice centromeres. | plant and animal centromeres comprise megabases of highly repeated satellite sequences, yet centromere function can be specified epigenetically on single-copy dna by the presence of nucleosomes containing a centromere-specific variant of histone h3 (cenh3). we determined the positions of cenh3 nucleosomes in rice (oryza sativa), which has centromeres composed of both the 155-bp cento satellite repeat and single-copy non-cento sequences. we find that cenh3 nucleosomes protect 90-100 bp of dna fro ... | 2013 | 24191062 |
| genetic variation detected with rapd markers among upland and lowland rice cultivars (oryza sativa l.). | genetic variation of nine upland and four lowland rice cultivars (oryza sativa l.) was investigated at the dna level using the randomly amplified polymorphic dna (rapd) method via the polymerase chain reaction (pcr). forty-two random primers were used to amplify dna segments and 260 pcr products were obtained. the results of agarosegel electrophoretic analysis of these pcr products indicated that 208 (80%) were polymorphic. all 42 primers used in this experiment were amplified and typically gene ... | 1994 | 24190409 |
| comparative studies of isozymes in oryza sativa, o. minuta, and their interspecific derivatives: evidence for homoeology and recombination. | enzyme electrophoresis was used to compare the isozyme phenotypes of oryza sativa, ir31917 (aa genome), and two o. minuta accessions (om 101089 and om101141; bbcc genome) for ten enzyme systems. between the two species, two systems were monomorphic (isocitrate dehydrogenase and alcohol dehydrogenase) and eight were polymorphic (shikimate dehydrogenase, phosphogluconate dehydrogenase, phosphoglucose isomerase, malate dehydrogenase, glutamate oxaloacetate transaminase, esterase, aminopeptidase, an ... | 1993 | 24190357 |
| totipotency of coleoptile tissue in indica rice (oryza sativa l. cv. ch 1039). | embryogenic and non-embryogenic calluses were induced from 3,4,5 and 7d old coleoptile segments of indica rice (oryza sativa l. cv. ch 1039). compact, globular, yellow and creamy embryogenic and white friable non-embryogenic callus arose from the cut end and entire length of the coleoptile segments. murashige and skoog's (ms) medium supplemented with 2.5mg/1 2,4-d was used as callus induction medium. plant regeneration from coleoptile segments occurred with the transfer of embryogenic callus to ... | 1995 | 24190304 |
| transient expression of gus gene in intact seed embryos of indica rice after electroporation-mediated gene delivery. | two-day-old germinating intact seed embryos of oryza sativa variety basmati 370 were electroporated with a view to examine suitability of this system for gene delivery. the experiments were done with a plasmid having gus gene under the control of camv 35s promoter. spectrofluorophotometric gus assay revealed high activity of the introduced gene when embryos were given three electrical pulses at 1600 v cm(-1) and 100 μf capacitance with a pulse length of 75 ms. additionally, histochemical localiz ... | 1995 | 24190298 |
| cryopreserved callus: a source of protoplasts for rice transformation. | we cryopreserved whole rice calli (oryza sativa l cv taipei 309) to investigate the ability of the surviving cells to regenerate plants and yield protoplasts competent for genetic transformation. four out of six callus lines cryopreserved after four months in culture contained small sectors able to continue cell division and subsequently regenerate fertile plants. both cryopreservation efficiency and regeneration ability decreased when using eight month old cultures. high yields of protoplasts w ... | 1995 | 24190297 |
| a rice (oryza sativa l.) mutant having a low content of glutelin and a high content of prolamine. | among the mutant lines of rice that have been selected for morphological characters, one line, nm67, was found to have a low content of glutelin and a higher content of prolamine in its seed protein than other japanese cultivars. this mutant is a semi-dwarf and partially sterile line, and its leaves turn yellow before heading. genetic analysis after backcross to the original cultivar, 'nihonmasari', revealed the following: (1) the character of low glutelin content was always accompanied by the c ... | 1993 | 24190265 |
| spatial transcriptomes of iron-deficient and cadmium-stressed rice. | although the genes involved in metal homeostasis have been investigated over the past few decades, many genes related to metal homeostasis remain uncharacterized, and a comprehensive analysis of the expression of these genes is required. in the present study, we investigated the spatial gene expression profile of iron (fe)-deficient and cadmium (cd)-stressed oryza sativa (rice) using laser microdissection and microarray analysis. roots of fe-deficient and cd-stressed rice were separated into the ... | 2014 | 24188410 |
| effect of cerium oxide nanoparticles on the quality of rice ( oryza sativa l.) grains. | despite the remarkable number of publications on the interaction of engineered nanoparticles (enps) with plants, knowledge of the implications of enps in the nutritional value of food crops is still limited. this research was performed to study the quality of rice grains harvested from plants grown in soil treated with cerium oxide nanoparticles (nceo2). three rice varieties (high, medium, and low amylose) were cultivated to full maturity in soil amended with nceo2 at 0 and 500 mg kg(-1) soil. c ... | 2013 | 24188281 |
| a rapid method to monitor dna precipitation onto microcarriers before particle bombardment. | the binding or precipitation of dna onto gold or tungsten microcarriers represents one of the most crucial steps for gene transfer via the particle bombardment process. we have developed a simple and rapid method to monitor dna precipitation onto microcarriers before delivery to intact cells or tissues. binding of dna constructs to different microcarriers was evaluated with relative fluorescence values using a dedicated fluorometer. significantly greater precipitation was detected using gold vs. ... | 1995 | 24186715 |
| plant regeneration from rice (oryza sativa l.) embryogenic suspension cells cryopreserved by vitrification. | rice cells were precultured for 10 d in medium containing 60 g/l sucrose and subsequently for 1 d in medium supplemented with 0. 4 m sorbitol. after loading with 25%pvs2 at 22°c for 10 min and dehydration in 100%pvs2 at 0°c for 7. 5 min,they were plunged into liquid nitrogen directly. survival was 45. 0 ±5.1% (based on the reduction of triphenyl tetrazolium chloride)following warming and unloading. for regrowth, cells were plated on semi-solid medium replenished with 40%(w/v) starch for 2d prior ... | 1995 | 24186632 |
| efficient plant regeneration from long-term callus cultures of rice by spermidine. | a significant reduction in regeneration potential with increasing age (upto 12months) in rice (oryza sativa l. cv.tn-1) embryogenic callus cultures was observed. spermidine, while having an inhibitory effect on plant regeneration in fresh callus cultures, promoted morphogenesis in long-term callus cultures. a massive accumulation of polyamines, particularly putrescine (5-fold) was observed in 12 month old cultures resulting in a change of putrescine /spermidine ratio, which seems to be important ... | 1995 | 24186629 |
| mapping of a magnaporthe grisea locus affecting rice (oryza sativa) cultivar specificity. | magnaporthe grisea causes rice blast, the most important fungal disease of rice. the segregation of genes controlling virulence of m. grisea on rice was studied to establish the genetic basis of cultivar specificity in this host-parasite interaction. full-sib progeny and parent isolates guy11 and 2539 of m. grisea were inoculated onto rice (oryza sativa) cultivar 'co39' and five near-isogenic lines (nils) of co39. each nil contained a different single gene affecting resistance to specific isolat ... | 1994 | 24186240 |
| identification of the duplicated segments in rice chromosomes 1 and 5 by linkage analysis of cdna markers of known functions. | we mapped two loci for adp-ribosylation factor homologues (arf1, arf2) and two loci for cysteine proteinase inhibitors (oryzacystatin-i and -ii: oci, ocii) by linkage analysis of restriction fragment length polymorphism loci in rice (oryza sativa l.) genomic dnas using their cdnas as probes.oc-1 andarf-2 were found to be closely located to each other on chromosome 1, whileoc-2 andarf-1,both found on chromosome 5, were also located close to each other. the map distances are about 2 cm in both pai ... | 1994 | 24186168 |
| rflp-based phylogenetic analysis of wide compatibility varieties in oryza sativa l. | twenty-one wide compatibility varieties (wcvs) of rice together with three indica and three japonica testers were assayed with 160 dna probes that were selected to represent the entire rflp map at an average interval of 11 cm. on the basis of four enzyme digestion 125 probes detected polymorphisms among the wcvs and subspecies' testers. among these polymorphic probes there were 68 that could distinguish the indica from the japonica testers. two dendrograms were constructed on the basis of 398 po ... | 1994 | 24185883 |
| plant regeneration from protoplasts of cytoplasmic male sterile lines of rice (oryza sativa l.). | this study compared plant regeneration from protoplasts isolated from suspension cultures of threejaponica rice (oryza sativa l.) lines with different male sterile cytoplasms. more than 180 green plants were regenerated from protoplasts from 5-8 month old suspensions of ir58024a, a line with the wa type of cytoplasmic male sterility (cms). about 40% of the calli recovered from protoplasts produced green plants. shuangbaia (bt type of cms) and tai2a (dian i type of cms), both from zhejiang provin ... | 1995 | 24185659 |
| evidence for the involvement of glycanase activities in the dissociation of cortical cell walls during the emergence of callus from rice root tissues in the presence of 2,4-d. | nojirimycin bisulfite andp-nitrophenyl-1-thio-ß-d-glucoside, potent inhibitors of ß-glucanase, suppressed cortical dissociation and callus growth in rice roots (oryza sativa l. cv. sasanishiki) in the presence of 2,4-d, with resultant suppression of the emergence of callus clumps from the interior of root tissues. these sugar analogs inhibited the 2,4-d-induced increase in the elastic and plastic compliance of cell walls but did not affect oxygen uptake by root explants in the presence of 2,4-d. ... | 1995 | 24185652 |
| suspension and protoplast culture of u.s. rice cultivars. | efficient protoplast culture and plant regeneration of five u.s. rice cultivars (oryza sativa l.) - mercury, lacassine, maybelle, cypress, and lemont - were obtained from suspension cells maintained in modified general medium. embryogenic suspension cells were developed from calli grown on the original callus induction medium for 10-20 weeks without subculture. weekly subculture of the suspensions for five to eight weeks yielded cells suitable for protoplast isolation. after 2 weeks, rate of col ... | 1995 | 24185650 |
| expression of rice osh1 gene is localized in developing vascular strands and its ectopic expression in transgenic rice causes altered morphology of leaf. | transgenic rice plants (oryza sativa cv. nipponbare) carrying 1 or 2 copies of a rice homeobox gene, osh1, under the control of the camv 35s promoter were generated. the transgene caused altered morphology of leaf, such as ligule-replacement and abnormal division of sclerenchyma cells. the phenotype of these leaves resembles that of maize leaf morphological mutant, knotted 1, which is caused by duplication of the kn1 gene (veit et al., 1990). the in situ hybridization analysis has revealed that ... | 1995 | 24185596 |
| rice anther culture: callus initiation and androclonal variation in progenies of regenerated plants. | anthers from rice (oryza sativa l.) subspecies japonica initiated more callus than their indica or indica x japonica counterparts. a mild stress, either by slow desiccation or heat shock, prior to the plating of anthers enhanced the ability to initiate callus. slow dessication of anthers enhanced the ability of the japonica anthers to initiate callus even in medium that was supplemented with nacl. the ability to initiate callus by the anthers plated on nacl-supplemented medium decreased as the n ... | 1995 | 24185451 |
| effect of culture methods on the regeneration of albino rice (oryza sativa l.) plantlets. | in our study, we investigated the effects of regeneration conditions on both green and albino rice plants (oryza sativa l.). the regeneration frequency of an albino cell line was compared to a normal cell line obtained from mature seed under two kinds of culture conditions; namely, the static culture on semi-solid regeneration medium and the suspension culture in liquid regeneration medium. the albino cell line, from which only albino plantlets were regenerated, was induced from the albino leaf ... | 1996 | 24178525 |
| effect of anoxia on starch breakdown in rice and wheat seeds. | the capabilities of rice (oryza sativa l.) and wheat (triticum aestivum l.) seeds (caryopses) to degrade starchy reserves present in the endosperm tissue were compared under anaerobic conditions. the results showed that rice, a species highly tolerant to anoxia, can readily break down starch under anaerobiosis concomitant with germination, while wheat does not germinate and fails to degrade starch present in the endosperm. this clearly distinct behavior is likely the consequence of the successfu ... | 1992 | 24178396 |
| gibberellin-induced growth and regulation of the cell division cycle in deepwater rice. | excised stem sections of deepwater rice (oryza sativa l., cv. pin gaew 56) containing the highest internode were used to study induction of rapid internodal elongation by gibberellin. it has been shown previously that this growth response is based on an increased cell production rate in the intercalary meristem and on increased cell elongation. our investigations were aimed at establishing the temporal sequence of these ga-regulated processes. cell sizes were determined by scanning electron micr ... | 1992 | 24178326 |
| variation in free-radical damage in rice cell suspensions with different embryogenic potentials. | levels of free-radical-mediated lipid peroxidation were monitored in cell-suspension cultures of oryza sativa l. possessing different embryogenic potentials. oxidative stress was evaluated using assays which sequentially assessed the stages of lipid peroxidation (diene conjugation, peroxidation, and the formation of secondary lipid-peroxidation products). lipid peroxidation was significantly higher in a cell line which had lost embryogenic ability compared with lines which still retained this ca ... | 1992 | 24178318 |
| transformation of haploid, microspore-derived cell suspension protoplasts of rice (oryza sativa l.). | we compared the transient activity of three cereal gene-derived promoter-gus fusions and the efficiency of selection mediated by three different selectable genes in a polyethylene glycol transformation system with haploid cell suspension protoplasts of rice. the maize ubiquitin promoter was found to be the most active in transformed protoplasts, and selection on ammonium glufosinate mediated by the bar gene was the most efficient for producing resistant calluses. cotransformation of protoplasts ... | 1996 | 24178168 |
| effect of oxygen-enriched aeration on regeneration of rice (oryza sativa l.) cell culture. | the effect of the oxygen concentration in the aeration gas on regeneration from rice cells in bioreactor cultures was investigated. the efficiency of regeneration in cultures aerated with over 40% oxygen was higher than that in a flask culture. in the case of a culture in which the dissolved oxygen(do) was saturated by aeration with air, the efficiency of regeneration was less than the half that of cultures aerated with 40% oxygen. in cultures with the do levels controlled at 8,10 and 12 mg/ℓ, t ... | 1996 | 24178160 |
| production and analysis of asymmetric hybrid plants between monocotyledon (oryza sativa l.) and dicotyledon (daucus carota l.). | asymmetric hybrid plants were obtained from fused protoplasts of a monocotyledon (oryza sativa l.) and a dicotyledon (daucus carota l.). x-ray-irradiated protoplasts isolated from a cytoplasmic malesterile (cms) carrot suspension culture were fused with iodoacetoamide-treated protoplasts isolated from a 5-methyltryptophan (5mt)-resistant rice suspension culture by electrofusion. the complementary recovered cells divided and formed colonies, which were then cultivated on regeneration medium suppl ... | 1994 | 24177855 |
| genetic analysis of tolerance for phosphorous deficiency in rice (oryza sativa l.). | the inheritance of phosphorous (p) - deficiency tolerance in rice was investigated by a sevenparent diallel. the parent materials involved were four p-efficient (ir20, ir54, ir28, and mahsuri), one moderately p-efficient (tn1), and two p-inefficient (ir31406333-1 and ir34686-179-1-2-1), genotypes. relative tilering ability (rta) under p-deficient and p-supplemented soil conditions was the parameter used in determining the tolerance level of the different genotypes. diallel graph analysis reveale ... | 1994 | 24177847 |
| the semidwarf gene, sd-1, of rice (oryza sativa l.). ii. molecular mapping and marker-assisted selection. | to establish the location of the semidwarf gene, sd-1, the anthocyanin activator (a), purple node (pn), purple auricle (pau), and the isozyme locus, esti-2, in relation to dna markers on the molecular linkage map of rice, 20 rflp markers, previously mapped to the central region of chromosome 1 (mccouch et al. 1988), were mapped onto an f2 population derived from the cross taichung 65 (a,pn,pau)/taichung 65 (sd-1). sd-1 and esti-2 were determined to be linked most tightly to rflp markers rg 109 a ... | 1994 | 24177769 |
| the semidwarf gene, sd-1, of rice (oryza sativa l.). i. linkage with the esterase locus, estl-2. | the linkage relationship between the semidwarf gene (sd-1) and the isozyme locus esti-2 was elucidated using segregating populations derived from crosses between several semidwarf testers and tall rice varieties. bimodal distributions for culm length were observed in f2 populations of three cross combinations, including shiokari/shiokari (sd-1), taichung 65 (a,pn,pau)/taichung 65 (sd-1), and milyang 23/kasalath. taking the valley of the distribution curves as the dividing point, two height class ... | 1994 | 24177768 |
| classification of cultivated rices into indica and japonica types by the isozyme, rflp and two milled-rice methods. | four methods for classifying cultivated rices (oryza sativa l.) (including ir varieties) into indica and japonica types - waxy gene product in endosperm starch, glutelin α3 molecular weight in milled rice, rflp polymorphism at the wx locus and glaszmann's isozyme method - were compared. on the basis of the two endosperm traits and the rflp method glaszmann's group 1 (indica) was classified as mainly indica and intermediate groups 2, 3 and 4 as exclusively indica. however, the endosperm traits cl ... | 1994 | 24177763 |
| nonstructural protein p7-2 encoded by rice black-streaked dwarf virus interacts with skp1, a core subunit of scf ubiquitin ligase. | rice black-streaked dwarf virus (rbsdv), a member of the genus fijivirus within the family reoviridae, causes severe damage to cereal crops in south east asia. the protein p7-2, encoded by the second open reading frame of segment s7, is conserved among most plant-infecting fijiviruses, but its function is still obscure. | 2013 | 24176102 |
| structural changes in the plastid dna of rice (oryza sativa l.) during tissue culture. | to investigate the rearrangement of the plastid genome during tissue culture, dna from rice callus lines, which had been derived individually from single protoplasts isolated from seed or pollen callus (protoclones), was analyzed by southern hybridization with rice chloroplast dna (ctdna) clones as probes. among 44 long-term cultured protoclones, maintained for 4, 8 or 11 years, 28 contained plastid dna (ptdna) from which portions had been deleted. the ptdna of all protoclones that had been main ... | 1995 | 24173926 |
| accumulation and translocation of 198hg in four crop species. | the uptake and transport of mercury (hg) through vegetation play an important role in the biogeochemical cycling of hg. however, quantitative information regarding hg translocation in plants is poorly understood. in the present study, hg uptake, accumulation, and translocation in 4 crops-rice (oryza.sativa l.), wheat (triticum l.), corn (zea mays l.), and oilseed rape (brassica campestris l.)-grown in hoagland solution were investigated using a stable isotope ((198)hg) tracing technique. the dis ... | 2014 | 24173818 |
| the physical location of fourteen rflp markers in rice (oryza sativa l.). | a biotin-labeled in situ hybridization technique was used in order to physically map rflp markers to the chromosomes of rice (oryza sativa l.). fourteen rflp markers, associated with the ends of the linkage groups on rice chromosomes 7, 8, 11, 12, were physically mapped onto specific regions of the chromosomes. the average detection rate of in situ hybridization was 5.91%. the markers were located on seven different chromosome arms. ten of the fourteen markers were distributed near the chromosom ... | 1995 | 24173791 |
| ascorbate metabolism in rice genotypes differing in zinc efficiency. | effects of zinc (zn) deficiency on shoot metabolites were investigated in contrasting rice (oryza sativa l.) genotypes with special focus on ascorbic acid (asa) biosynthesis, recycling, and catabolism. the genotypes ir74 (sensitive) and ril46 (tolerant) were subjected to -zn and control treatments for 3 weeks, and samples were taken at three different stages representing the pre-stress phase, emergence of visible stress, and severe visible stress. the emergence of visible symptoms was paralleled ... | 2014 | 24173698 |
| variation in protein and rna synthesis activity in isolated mitochondria of the developing rice (oryza sativa l.) panicle. | we have studied variation in mitochondrial protein and rna synthesis during the development of a specialized rice (oryza sativa l.) reproductive organ in a bacteria-free environment. mitochondria were prepared from the maturing panicle during microsporogenesis when meiosis occurred and from etiolated seedlings at two growth stages. we found (1) that there was no discernible qualitative difference among the polypeptides synthesized by these three mitochondrial samples; (2) that the quantity of pr ... | 1995 | 24173071 |
| the effects of selection for sodium transport and of selection for agronomic characteristics upon salt resistance in rice (oryza sativa l.). | a multiple cross was constructed with the aim of combining component traits for the complex salinity resistance character. the aim was to combine donors for physiological traits with the agronomically desirable semidwarf/intermediate plant type and with the overall salinity resistance of the traditional tall land races. we report a study of selection strategies in the resulting breeding population. the effects of early selection for agronomic traits and early selection for low sodium transport w ... | 1995 | 24173070 |
| the identification of duplicate accessions within a rice germplasm collection using rapd analysis. | a set of accessions of oryza sativa from the international rice research institute (philippines) that included known and suspected duplicates as well as closely related germplasm has been subjected to rapd analysis. the number of primers, the number of polymorphic bands and the total number of bands were determined that will allow the accurate discrimination of these categories of accessions, including the identification of true and suspected duplicates. two procedures have been described that c ... | 1995 | 24173061 |
| a chromosome 5-specific repetitive dna sequence in rice (oryza sativa l). | repetitive dna sequences in the rice genome comprise more than half of the nuclear dna. the isolation and characterization of these repetitive dna sequences should lead to a better understanding of rice chromosome structure and genome organization. we report here the characterization and chromosome localization of a chromosome 5-specific repetitive dna sequence. this repetitive dna sequence was estimated to have at least 900 copies. dna sequence analysis of three genomic clones which contain the ... | 1995 | 24173043 |
| identification of a set of rflp probes for subspecies differentiation in oryza sativa l. | sixty-eight indica-japonica tester-differentiating rflp probes were tested in seven indica and seven japonica varieties of rice (oryza sativa l.) with four enzyme digestions (ecori, ecorv, hindiii and drai). twenty-one dna clones were isolated as indica-japonica subspecies-differentiating probes. a set of 13 probes was established as core probes for subspecies differentiation and a pooled blotting analysis was carried out to facilitate the application of rflp in rice genetics and breeding practi ... | 1995 | 24172933 |
| genome-wide binding analysis of the transcription activator ideal plant architecture1 reveals a complex network regulating rice plant architecture. | ideal plant architecture1 (ipa1) is critical in regulating rice (oryza sativa) plant architecture and substantially enhances grain yield. to elucidate its molecular basis, we first confirmed ipa1 as a functional transcription activator and then identified 1067 and 2185 genes associated with ipa1 binding sites in shoot apices and young panicles, respectively, through chromatin immunoprecipitation sequencing assays. the squamosa promoter binding protein-box direct binding core motif gtac was highl ... | 2013 | 24170127 |
| tagging and mapping the thermo-sensitive genic male-sterile gene in rice (oryza sativa l.) with molecular markers. | the thermo-sensititve genic male-sterile (tgms) gene in rice can alter fertility in response to temperature and is useful in the two-line system of hybrid rice production. however, little is known about the tgms gene at the molecular level. the objective of this study was to identify molecular markers tightly linked with the tgms gene and to map the gene onto a specific rice chromosome. bulked segregant analysis of an f2 population from 5460s (a tgms mutant line) x 'hong wan 52' was used to iden ... | 1995 | 24170005 |
| genetic diversity and phylogeny analysis of anabaena azollae based on rflps detected in azolla-anabaena azollae dna complexes using nif gene probes. | the cyanobacterium anabaena has both symbiotic and free-living forms. the genetic diversity of anabaena strains symbiotically associated with the aquatic fern azolla and the evolutionary relationships among these symbionts were evaluated by means of rflp (restriction fragment length polymorphism) experiments. three dna fragments corresponding to nif genes were cloned from the free-living cyanobacterium anabaena pcc 7120 and used as probes. a mixture of azolla, anabaena and bacterial dna was extr ... | 1995 | 24169885 |
| genetic variation detected by dna fingerprinting with a rice minisatellite probe in oryza sativa l. | a rice minisatellite probe detecting dna fingerprints was used to assess genetic variation in cultivated rice (oryza sativa l.). fifty-seven cultivars of rice, including 40 closely related cultivars released in the us, were studied. rice dna fingerprinting revealed high levels of polymorphism among distantly related cultivars. the variability of fingerprinting pattern was reduced in the closely related cultivars. a genetic similarity index (s) was computed based on shared fragments between each ... | 1995 | 24169839 |