Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| improvement of capture efficacy of immunomagnetic beads for campylobacter jejuni using reagents that alter its motility. | previous studies using the immunomagnetic beads separation (ims) technique have shown high detection limits of live campylobacters but low detection limits of formalin-killed campylobacters. the present study investigated if the addition of various concentrations of reagents that alter the motility of live campylobacter jejuni could enhance the recovery of the organisms by ims. the addition of 5% glycerol, 0.001% formalin, 10% polyethylene glycol, or 0.001% agarose in a buffer slowed down the mo ... | 2013 | 23826961 |
| global distribution of campylobacter jejuni penner serotypes: a systematic review. | penner serotyping has been the principal method for differentiating campylobacter isolates since its inception. campylobacter capsule polysaccharide (cps), the principal serodeterminant on which penner serotyping is based, is presently of interest as a vaccine component. to determine the required valency of an effective cps-based vaccine, a comprehensive understanding of cps distribution is needed. because of the association between penner serotype and cps, we conducted a systematic review to es ... | 2013 | 23826280 |
| comparative genomics reveal that host-innate immune responses influence the clinical prevalence of legionella pneumophila serogroups. | legionella pneumophila is the primary etiologic agent of legionellosis, a potentially fatal respiratory illness. amongst the sixteen described l. pneumophila serogroups, a majority of the clinical infections diagnosed using standard methods are serogroup 1 (sg1). this high clinical prevalence of sg1 is hypothesized to be linked to environmental specific advantages and/or to increased virulence of strains belonging to sg1. the genetic determinants for this prevalence remain unknown primarily due ... | 2013 | 23826259 |
| rapid detection and simultaneous genotyping of cronobacter spp. (formerly enterobacter sakazakii) in powdered infant formula using real-time pcr and high resolution melting (hrm) analysis. | cronobacter spp. is an emerging pathogen that causes meningitis, sepsis, bacteremia, and necrotizing enterocolitis in neonates and children. the present study developed an assay integrating real-time pcr and high resolution melting (hrm) analysis targeting the ompa gene for the specific detection and rapid identification of cronobacter spp. (formerly enterobacter sakazakii) in powdered infant formula. eleven cronobacter field isolates and 25 reference strains were examined using one pair of prim ... | 2013 | 23825624 |
| bacterial mediated gastrointestinal inflammation. | mouse models have proven to be a key approach in our understanding of the etiology and physiology underlying bacterial mediated gastrointestinal inflammation. generally, these models are based on the inoculation of genetically susceptible mice with either commensal or pathogenic bacteria to elicit an inflammatory response. here, we describe models of acute and chronic gastrointestinal inflammation using interleukin 10-deficient (il10 (-/-)) mice colonized with the pathogenic campylobacter jejuni ... | 2013 | 23824902 |
| clinical application of volatile organic compound analysis for detecting infectious diseases. | this review article introduces the significance of testing of volatile organic compounds (vocs) in clinical samples and summarizes important features of some of the technologies. compared to other human diseases such as cancer, studies on voc analysis in cases of infectious diseases are limited. here, we have described results of studies which have used some of the appropriate technologies to evaluate voc biomarkers and biomarker profiles associated with infections. the publications reviewed inc ... | 2013 | 23824368 |
| myocarditis associated with campylobacter jejuni. | myocarditis is frequently associated with a multitude of different viral infections but much less commonly a bacterial source. we present the case of a 33-year-old male with campylobacter jejuni enteritis who subsequently developed myocarditis confirmed on cardiac mri. | 2013 | 23824028 |
| neutrophil activation by campylobacter concisus. | campylobacter concisus is an emerging enteric pathogen associated with prolonged diarrhoea and possibly inflammatory bowel disease in children as well as adults, but the interaction with cells of the innate immune system is unclear. the magnitude of systemic immunoglobulin response in acute infection is unknown. | 2013 | 23819746 |
| extreme genetic fragility of the hiv-1 capsid. | genetic robustness, or fragility, is defined as the ability, or lack thereof, of a biological entity to maintain function in the face of mutations. viruses that replicate via rna intermediates exhibit high mutation rates, and robustness should be particularly advantageous to them. the capsid (ca) domain of the hiv-1 gag protein is under strong pressure to conserve functional roles in viral assembly, maturation, uncoating, and nuclear import. however, ca is also under strong immunological pressur ... | 2013 | 23818857 |
| genome-wide association study identifies vitamin b5 biosynthesis as a host specificity factor in campylobacter. | genome-wide association studies have the potential to identify causal genetic factors underlying important phenotypes but have rarely been performed in bacteria. we present an association mapping method that takes into account the clonal population structure of bacteria and is applicable to both core and accessory genome variation. campylobacter is a common cause of human gastroenteritis as a consequence of its proliferation in multiple farm animal species and its transmission via contaminated m ... | 2013 | 23818615 |
| [accuracy of etest method to study campylobacter spp. susceptibility to erythromycin, ciprofloxacin and tetracycline]. | in industrialized countries campylobacter jejuni is the enteropathogen most frequently isolated from the feces of patients with gastroenteritis. the etest accuracy to categorize campylobacter spp. susceptibility to erythromycin, ciprofloxacin and tetracycline was evaluated. | 2013 | 23817649 |
| [incidence and susceptibility of campylobacter jejuni in pediatric patients: involvement in bacteremia]. | invasive disease as a result campylobacter spp. is rarely reported. bloodstream infections have been reported in patients with immune deficiency or other serious underlying conditions. we conducted a prospective study to know the incidence of campylobacter jejuni bacteremia in pediatric patients and its susceptibility to erythromycin and ciprofloxacin. | 2013 | 23817644 |
| mntabc and mnth contribute to systemic staphylococcus aureus infection by competing with calprotectin for nutrient manganese. | during infection, vertebrates limit access to manganese and zinc, starving invading pathogens, such as staphylococcus aureus, of these essential metals in a process termed "nutritional immunity." the manganese and zinc binding protein calprotectin is a key component of the nutrient-withholding response, and mice lacking this protein do not sequester manganese from s. aureus liver abscesses. one potential mechanism utilized by s. aureus to minimize host-imposed manganese and zinc starvation is th ... | 2013 | 23817615 |
| synergistic effects of anti-cmea and anti-cmeb peptide nucleic acids on sensitizing campylobacter jejuni to antibiotics. | the cmeabc efflux pump in campylobacter jejuni confers resistance to structurally divergent antimicrobials, and inhibition of cmeabc represents a promising strategy to control antibiotic-resistant campylobacter. antisense peptide nucleic acids (pnas) targeting the three components of cmeabc were evaluated for inhibition of cmeabc expression. the result revealed a synergistic effect of the pnas targeting cmea and cmeb on sensitizing c. jejuni to antibiotics. this finding further demonstrates the ... | 2013 | 23817373 |
| free-ranging chickens in households in a periurban shantytown in peru--attitudes and practices 10 years after a community-based intervention project. | free-ranging chickens are often found in periurban communities in developing countries, and their feces can pose a significant public health sanitation problem. corralling chickens raised in these periurban areas in chicken coops has been proposed previously as an intervention to address this problem. aims of this study were to revisit households in a corralling intervention study conducted in 2000-2001 to compare poultry-raising practices and investigate current attitudes regarding the impact o ... | 2013 | 23817335 |
| fluorescent probes for investigation of isoprenoid configuration and size discrimination by bactoprenol-utilizing enzymes. | undecaprenyl pyrophosphate synthase (upps) is an enzyme critical to the production of complex polysaccharides in bacteria, as it produces the crucial bactoprenol scaffold on which these materials are assembled. methods to characterize the systems associated with polysaccharide production are non-trivial, in part due to the lack of chemical tools to investigate their assembly. in this report, we develop a new fluorescent tool using upps to incorporate a powerful fluorescent anthranilamide moiety ... | 2013 | 23816045 |
| crystal structure of the c-terminal globular domain of the third paralog of the archaeoglobus fulgidus oligosaccharyltransferases. | protein n-glycosylation occurs in the three domains of life. oligosaccharyltransferase (ost) transfers an oligosaccharide chain to the asparagine residue in the n-glycosylation sequons. the catalytic subunits of the ost enzyme are stt3 in eukaryotes, aglb in archaea and pglb in eubacteria. the genome of a hyperthermophilic archaeon, archaeoglobus fulgidus, encodes three paralogous aglb proteins. we previously solved the crystal structures of the c-terminal globular domains of two paralogs, aglb- ... | 2013 | 23815857 |
| a subgraph isomorphism algorithm and its application to biochemical data. | graphs can represent biological networks at the molecular, protein, or species level. an important query is to find all matches of a pattern graph to a target graph. accomplishing this is inherently difficult (np-complete) and the efficiency of heuristic algorithms for the problem may depend upon the input graphs. the common aim of existing algorithms is to eliminate unsuccessful mappings as early as and as inexpensively as possible. | 2013 | 23815292 |
| role of the gut microbiota in health and chronic gastrointestinal disease: understanding a hidden metabolic organ. | the human gut microbiota has become the subject of extensive research in recent years and our knowledge of the resident species and their potential functional capacity is rapidly growing. our gut harbours a complex community of over 100 trillion microbial cells which influence human physiology, metabolism, nutrition and immune function while disruption to the gut microbiota has been linked with gastrointestinal conditions such as inflammatory bowel disease and obesity. here, we review the many s ... | 2013 | 23814609 |
| antimicrobial resistance in the food chain: a review. | antimicrobial resistant zoonotic pathogens present on food constitute a direct risk to public health. antimicrobial resistance genes in commensal or pathogenic strains form an indirect risk to public health, as they increase the gene pool from which pathogenic bacteria can pick up resistance traits. food can be contaminated with antimicrobial resistant bacteria and/or antimicrobial resistance genes in several ways. a first way is the presence of antibiotic resistant bacteria on food selected by ... | 2013 | 23812024 |
| measurement of predation and biofilm formation under different ambient oxygen conditions using a simple gasbag-based system. | bdellovibrio bacteriovorus and micavibrio aeruginosavorus are gram-negative bacteria characterized by predatory behavior. the aim of this study was to evaluate the ability of the predators to prey in different oxygen environments. when placed on an orbital shaker, a positive association between the rate of aeration and predation was observed. to further examine the effects of elevated ambient oxygen levels on predation, a simple gasbag system was developed. using the system, we were able to cond ... | 2013 | 23811501 |
| short communication: survey of animal-borne pathogens in the farm environment of 13 dairy operations. | a survey was conducted on 13 dairies to determine the occurrence of 5 animal-borne pathogens (salmonella enterica, escherichia coli o157:h7, campylobacter jejuni, mycobacterium avium ssp. paratuberculosis, and cryptosporidium parvum) and their distributions across farm elements (feces, bedding, milk filters, stored manure, field soil, and stream water). presence of c. parvum was measured only in feces and stored manure. all but one farm were positive for at least one pathogen species, and 5 farm ... | 2013 | 23810596 |
| a surface-exposed neuraminidase affects complement resistance and virulence of the oral spirochaete treponema denticola. | neuraminidases (sialidases) catalyse the removal of terminal sialic acid from glycoconjugates. bacterial pathogens often utilize neuraminidases to scavenge host sialic acid, which can be utilized either as a nutrient or as a decorating molecule to disguise themselves from host immune attacks. herein, a putative neuraminidase (tde0471) was identified in treponema denticola, an oral spirochaete associated with human periodontitis. tde0471 is a cell surface-exposed exo-neuraminidase that removes si ... | 2013 | 23808705 |
| bacteria in the nose of young adults during wellness and rhinovirus colds: detection by culture and microarray methods in 100 nasal lavage specimens. | patients with viral respiratory infections/viral rhinitis/common colds are often treated with antibiotic; however, there is little information on whether or how bacterial microbiota in the nose and nasopharynx might influence the course of viral illnesses. | 2013 | 23801660 |
| a chemoenzymatic route to synthesize unnatural sugar nucleotides using a novel n-acetylglucosamine-1-phosphate pyrophosphorylase from camphylobacter jejuni nctc 11168. | a novel n-acetylglucosamine-1-phosphate pyrophosphorylase was identified from campylobacter jejuni nctc 11168. an unprecedented degree of substrate promiscuity has been revealed by systematic studies on its substrate specificities towards sugar-1-p and ntp. the yields of the synthetic reaction of seven kinds of sugar nucleotides catalyzed by the enzyme were up to 60%. in addition, the yields of the other nine were around 20%. with this enzyme, three novel sugar nucleotide analogs were synthesize ... | 2013 | 23800684 |
| targeting the bacteria-host interface: strategies in anti-adhesion therapy. | bacterial infections are a major cause of morbidity and mortality worldwide and are increasingly problematic to treat due to the rise in antibiotic-resistant strains. it becomes more and more challenging to develop new antimicrobials that are able to withstand the ever-increasing repertoire of bacterial resistance mechanisms. this necessitates the development of alternative approaches to prevent and treat bacterial infections. one of the first steps during bacterial infection is adhesion of the ... | 2013 | 23799663 |
| potential of a commercially available water acidification product for reducing campylobacter in broilers prior to slaughter. | 1. this study investigated the potential of a commercially available acidified water treatment (pwt) for reducing the number of campylobacter in vitro and other bacteria in the gut of live broilers. 2. in vitro tests indicated that pwt was highly effective for reducing campylobacter jejuni and campylobacter coli at the recommended concentration in water, reducing populations by greater than 7 log10 cfu/ml after 24 h exposure. the decrease in the number of salmonella serovar enteritidis and esche ... | 2013 | 23796116 |
| serotypes, virulence factors, and antimicrobial susceptibilities of vaginal and fecal isolates of escherichia coli from giant pandas. | although escherichia coli typically colonizes the intestinal tract and vagina of giant pandas, it has caused enteric and systemic disease in giant pandas and greatly impacts the health and survival of this endangered species. in order to understand the distribution and characteristics of e. coli from giant pandas, 67 fecal and 30 vaginal e. coli isolates from 21 giant pandas were characterized for o serogroups, phylogenetic groups, antimicrobial susceptibilities, and pulsed-field gel electrophor ... | 2013 | 23793635 |
| aggregatibacter actinomycetemcomitans leukotoxin utilizes a cholesterol recognition/amino acid consensus site for membrane association. | aggregatibacter actinomycetemcomitans produces a repeats-in-toxin (rtx) leukotoxin (ltxa) that selectively kills human immune cells. binding of ltxa to its β2 integrin receptor (lymphocyte function-associated antigen-1 (lfa-1)) results in the clustering of the toxin·receptor complex in lipid rafts. clustering occurs only in the presence of lfa-1 and cholesterol, and ltxa is unable to kill cells lacking either lfa-1 or cholesterol. here, the interaction of ltxa with cholesterol was measured using ... | 2013 | 23792963 |
| prevalence of campylobacter spp. relative to other enteric pathogens in grow-finish pigs with diarrhea. | salmonella spp., lawsonia intracellularis, and brachyspira spp. are pathogens commonly associated with diarrhea in growing and finishing pigs. brachyspira spp. infection has recently reemerged as a significant concern due to an increase in the incidence of swine dysentery; however, the mechanisms underlying this increase in dysentery remain largely unknown. pigs are also well-recognized as potential carriers of campylobacter spp., particularly campylobacter coli, yet enteric disease in swine ass ... | 2013 | 23792232 |
| distribution of nine virulence-associated genes in campylobacter jejuni and c. coli isolated from broiler feces in shiraz, southern iran. | to investigate the prevalence of nine virulence and toxin genes of campylobacter, a total of 90 campylobacter strains including 48 c. jejuni and 42 c. coli were recovered from chicken feces by cultivation methods. the isolates were identified on the basis of polymerase chain reaction (pcr) detection of 16srrna and multiplex pcr for determining two species. for confirmed strains, pcr was carried out for the presence of virulence genes using specific primers. data were analyzed by spss software, v ... | 2013 | 23789768 |
| absence of class 1 and class 2 integrons among campylobacter jejuni and campylobacter coli isolated from poultry in italy. | 2013 | 23788478 | |
| dynamics of fecal microbial communities in children with diarrhea of unknown etiology and genomic analysis of associated streptococcus lutetiensis. | the sequences of the 16s rrna genes extracted from fecal samples provide insights into the dynamics of fecal microflora. this potentially gives valuable etiological information for patients whose conditions have been ascribed to unknown pathogens, which cannot be accomplished using routine culture methods. we studied 33 children with diarrhea who were admitted to the children's hospital in shanxi province during 2006. | 2013 | 23782707 |
| [enteropathogens responsible for gastrointestinal disorders in hiv patients]. | gastrointestinal disorders or gid are debilitating conditions common in individuals infected by the human immunodeficiency virus (hiv), capable of leading to death. numerous etiological agents and pathophysiological mechanisms have been involved in this status. although the use of highly active antiretroviral therapy (haart) in many countries has greatly reduced the prevalence of gastrointestinal infections, enteric pathogens such as bacteria, parasites, fungi and viruses may still act as opport ... | 2013 | 23781716 |
| gastrointestinal pathogens in rodents overwintering in human facilities around uppsala, sweden. | rodents often live in close association with anthropogenic environments, offering rodent-associated pathogens numerous opportunities for transmission to humans. we screened for zoonotic gastrointestinal pathogens in rodents caught during autumn/winter in human dwellings in rural areas surrounding uppsala, sweden. we detected campylobacter jejuni, giardia sp., and cryptosporidium sp. in rodent intestines. | 2013 | 23778637 |
| shiga toxin-producing escherichia coli in yaks (bos grunniens) from the qinghai-tibetan plateau, china. | shiga toxin (stx)-producing escherichia coli (stec) are recognized as important human pathogens of public health concern. many animals are the sources of stec. in this study we determined the occurrence and characteristics of the stec in yaks (bos grunniens) from the qinghai-tibetan plateau, china. a total of 728 yak fecal samples was collected from june to august, 2012 and was screened for the presence of the stx 1 and stx 2 genes by taqman real-time pcr after the sample was enriched in modifie ... | 2013 | 23776496 |
| experimental evidence for the involvement of amino acid residue glu398 in the autocatalytic processing of bacillus licheniformis γ-glutamyltranspeptidase. | the role of glutamate 398 in the autocatalytic processing of bacillus licheniformis γ-glutamyltranspeptidase (blggt) was explored by site-directed mutagenesis. this glutamate was substituted by either alanine, aspartate, arginine or glutamine and the expressed mutant enzymes were purified to apparent homogeneity with metal-affinity chromatography. sds-page analysis showed that e398a, e398d and e398k were unable to process themselves into a large and a small subunit. however, e398q was not only a ... | 2012 | 23772362 |
| a multicopper oxidase is required for copper resistance in mycobacterium tuberculosis. | mycobacterium tuberculosis, the causative agent of tuberculosis, is one of the most important bacterial pathogens. recent work has revealed that the natural bactericidal properties of copper are utilized by the host immune system to combat infections with bacteria, including m. tuberculosis. however, m. tuberculosis employs multiple mechanisms to reduce the internal copper amount by efflux and sequestration, which are required for virulence of m. tuberculosis. here, we describe an alternative me ... | 2013 | 23772064 |
| massive lower gastrointestinal haemorrhage in a teenager caused by campylobacter enteritis. | a 14-year-old boy presented with a very unusual complication of massive lower gastrointestinal bleeding mimicking bleeding from meckel's diverticulum who needed blood transfusions and required mini-exploration. he was found to have campylobacter enteritis with bleeding from multiple mucosal ulcers in the ileocaecal region, which is presented with a review of the literature. | 2013 | 23771973 |
| adherence to and invasion of human intestinal cells by arcobacter species and their virulence genotypes. | the genus arcobacter is composed of 17 species which have been isolated from various sources. of particular interest are a. butzleri, a. cryaerophilus, and a. skirrowii, as these have been associated with human cases of diarrhea, the probable transmission routes being through the ingestion of contaminated drinking water and food. to date, only limited studies of virulence traits in this genus have been undertaken. the present study used 60 arcobacter strains isolated from different sources, repr ... | 2013 | 23770897 |
| adaptive mechanisms of campylobacter jejuni to erythromycin treatment. | macrolide is the drug of choice to treat human campylobacteriosis, but campylobacter resistance to this antibiotic is rising. the mechanisms employed by campylobacter jejuni to adapt to erythromycin treatment remain unknown and are examined in this study. the transcriptomic response of c. jejuni nctc 11168 to erythromycin (ery) treatment was determined by competitive microarray hybridizations. representative genes identified to be differentially expressed were further characterized by constructi ... | 2013 | 23767761 |
| nitric oxide reactivities of the two globins of the foodborne pathogen campylobacter jejuni: roles in protection from nitrosative stress and analysis of potential reductants. | during infection and pathogenesis, campylobacter, the leading cause of gastroenteritis, encounters no and reactive nitrogen species (rns) derived from the host. to combat these species, campylobacter jejuni expresses two haemoglobins: the single domain haemoglobin (cgb) detoxifies no but the role of the truncated globin (ctb) is unclear. confirmation of cgb activity and more extensive exploration of ctb function(s) in vivo are restricted due to difficulties in expressing proteins in campylobacte ... | 2013 | 23764490 |
| campylobacter jejuni in hospitalized patients with diarrhea, kolkata, india. | 2013 | 23763834 | |
| antibodies to glycoproteins shared by human peripheral nerve and campylobacter jejuni in patients with multifocal motor neuropathy. | we have tested serum samples from 24 patients with multifocal motor neuropathy (mmn) for reactivity to ganglioside gm1 and to gal( β 1-3)galnac-bearing glycoproteins isolated from human peripheral nerve and from campylobacter jejuni (cj) serotype o:19. igm anti-gm1 antibodies were detected by elisa in 11 patients (45.8%) with mmn and in only one subject (4%) from the control group. western blots showed positive reactivity of sera from 6 patients (25%) with mmn to several gal( β 1-3)galnac-bearin ... | 2013 | 23762534 |
| characterization of putative cholesterol recognition/interaction amino acid consensus-like motif of campylobacter jejuni cytolethal distending toxin c. | cytolethal distending toxin (cdt) produced by campylobacter jejuni comprises a heterotrimeric complex formed by cdta, cdtb, and cdtc. among these toxin subunits, cdta and cdtc function as essential proteins that mediate toxin binding to cytoplasmic membranes followed by delivery of cdtb into the nucleus. the binding of cdta/cdtc to the cell surface is mediated by cholesterol, a major component in lipid rafts. although the putative cholesterol recognition/interaction amino acid consensus (crac) d ... | 2013 | 23762481 |
| combination of aidp and pyramidal signs associated with antecedent hepatitis a infection: a rare (co)occurrence. | guillain-barré syndrome (gbs) is a postinfectious illness and commonly occurs in association with infective agents such as campylobacter jejuni, cytomegalovirus, epstein-barr virus, mycoplasma pneumoniae, hiv, shigella, clostridium, haemophilus influenzae; occasionally with acute hepatitis b, c, e; and more rarely with hepatitis a, as documented in various case reports. classically, gbs presents with hyporeflexia or areflexia, but preserved and brisk reflexes have been described in context with ... | 2013 | 23761608 |
| multiplex real-time pcr for detection of campylobacter, salmonella, and shigella. | infectious diarrhea can be classified based on its clinical presentation as noninflammatory or inflammatory disease. in developing countries, among inflammatory diarrhea cases, shigella is the most common cause, followed by campylobacter and salmonella. because the time frame in which treatment choices must be made is short and conventional stool cultures lack good sensitivity, there is a need for a rapid, sensitive, and inexpensive detection technique. the purpose of our study was to develop a ... | 2013 | 23761159 |
| improved detection of bacterial pathogens in patients presenting with gastroenteritis by use of the entericbio real-time gastro panel i assay. | in this study, we evaluated the use of entericbio real-time gastro panel i (serosep, limerick, ireland) for routine use in a clinical microbiology laboratory for simultaneous detection of campylobacter jejuni, coli, and lari, shiga toxin-producing escherichia coli (stec), salmonella spp., and shigella spp. in feces. this system differs from its predecessor (the entericbio panel ii system, serosep) in that it allows real-time detection of pathogens directly from feces, without pre-enrichment. it ... | 2013 | 23761157 |
| broad-spectrum biosensor capable of detecting and identifying diverse bacterial and candida species in blood. | we describe an assay which uses broad-spectrum, conserved-site pcr paired with mass spectrometry analysis of amplicons (pcr/electrospray ionization-mass spectrometry [esi-ms]) to detect and identify diverse bacterial and candida species in uncultured specimens. the performance of the assay was characterized using whole-blood samples spiked with low titers of 64 bacterial species and 6 candida species representing the breadth of coverage of the assay. the assay had an average limit of detection o ... | 2013 | 23761152 |
| phage biocontrol of enteropathogenic and shiga toxin-producing escherichia coli in meat products. | ten bacteriophages were isolated from faeces and their lytic effects assayed on 103 pathogenic and non-pathogenic enterobacteriaceae. two phages (dt1 and dt6) were selected based on their host ranges, and their lytic effects on pathogenic e. coli strains inoculated on pieces of beef were determined. we evaluated the reductions of viable cells of escherichia coli o157:h7 and non-o157 shiga toxigenic e. coli strains on meat after exposure to dt6 at 5 and 24°c for 3, 6, and 24 h and the effect of b ... | 2013 | 23761050 |
| the mycobacterium tuberculosis secreted protein rv0203 transfers heme to membrane proteins mmpl3 and mmpl11. | mycobacterium tuberculosis is the causative agent of tuberculosis, which is becoming an increasingly global public health problem due to the rise of drug-resistant strains. while residing in the human host, m. tuberculosis needs to acquire iron for its survival. m. tuberculosis has two iron uptake mechanisms, one that utilizes non-heme iron and another that taps into the vast host heme-iron pool. to date, proteins known to be involved in mycobacterial heme uptake are rv0203, mmpl3, and mmpl11. w ... | 2013 | 23760277 |
| a dna microarray for the versatile diagnosis of infectious diarrhea. | several bacteria, viruses, and parasites cause diarrhea as coinfecting pathogens. we designed a dna microarray comprising 60-bp probes spotted 194 times for the multiplex detection of 33 enteropathogenic bacteria and seven enteropathogenic viruses, and the archaeon methanobrevibacter smithii was used as an internal positive control. nine pathogen-free stool specimens were used as negative controls. one of these control specimens was further spiked with salmonella enterica as a positive control. ... | 2013 | 23758523 |
| orthopaedic manifestations and diagnostic clues in children with guillain-barré syndrome. | guillain-barré syndrome (gbs) is an acute inflammatory demyelinating polyneuropathy characterized by symmetric limb weakness. children with gbs sometimes consult the orthopaedists because of limb pain and gait disturbance. the orthopaedists, however, are unfamiliar with gbs, since it has rarely been delineated in detail in the orthopaedic literature. in the present study, we specifically describe orthopaedic manifestations and diagnostic clues in pediatric gbs. | 2013 | 23755090 |
| the antibiotic resistance "mobilome": searching for the link between environment and clinic. | antibiotic resistance is an ancient problem, owing to the co-evolution of antibiotic-producing and target organisms in the soil and other environments over millennia. the environmental "resistome" is the collection of all genes that directly or indirectly contribute to antibiotic resistance. many of these resistance determinants originate in antibiotic-producing organisms (where they serve to mediate self-immunity), while others become resistance determinants only when mobilized and over-express ... | 2013 | 23755047 |
| targeted identification of glycosylated proteins in the gastric pathogen helicobacter pylori (hp). | virulence of the gastric pathogen helicobacter pylori (hp) is directly linked to the pathogen's ability to glycosylate proteins; for example, hp flagellin proteins are heavily glycosylated with the unusual nine-carbon sugar pseudaminic acid, and this modification is absolutely essential for hp to synthesize functional flagella and colonize the host's stomach. although hp's glycans are linked to pathogenesis, hp's glycome remains poorly understood; only the two flagellin glycoproteins have been f ... | 2013 | 23754784 |
| characterization of intracellular growth regulator icgr by utilizing transcriptomics to identify mediators of pathogenesis in shigella flexneri. | shigella species gram-negative bacteria which cause a diarrheal disease, known as shigellosis, by invading and destroying the colonic mucosa and inducing a robust inflammatory response. with no vaccine available, shigellosis annually kills over 600,000 children in developing countries. this study demonstrates the utility of combining high-throughput bioinformatic methods with in vitro and in vivo assays to provide new insights into pathogenesis. comparisons of in vivo and in vitro gene expressio ... | 2013 | 23753632 |
| showers, sweating and suing: legionnaires' disease and 'new' infections in britain, 1977-90. | legionnaires' disease is now routinely discussed as an 'emerging infectious disease' (eid) and is said to be one of the earliest such diseases to be recognised. it first appeared in 1976 and its cause was identified in 1977, the same year that ebola fever, hantaan virus and campylobacter jejuni arrived. the designation of legionnaires' disease as an eid was retrospective; it was not and could not be otherwise as the category only gained currency in the early 1990s. in this article we reflect on ... | 2012 | 23752984 |
| biosynthesis of udp-n,n'-diacetylbacillosamine in acinetobacter baumannii: biochemical characterization and correlation to existing pathways. | the gram-negative, opportunistic pathogen acinetobacter baumannii has recently captured headlines due to its ability to circumvent current antibiotic therapies. herein we show that the multi-drug resistant (mdr) aye strain of a. baumannii contains a gene locus that encodes three enzymes responsible for the biosynthesis of the highly-modified bacterial nucleotide sugar, udp-n,n'-diacetylbacillosamine (udp-dinacbac). previously, this udp-sugar has been implicated in the pgl pathway of campylobacte ... | 2013 | 23747578 |
| clinical manifestations of campylobacter concisus infection in children. | there is only sparse information about the clinical impact of campylobacter concisus infections in children. | 2013 | 23743545 |
| comparison of polycarbonate and cellulose acetate membrane filters for isolation of campylobacter concisus from stool samples. | one thousand seven hundred ninety-one diarrheic stool samples were cultivated for campylobacter spp. we found a high prevalence of campylobacter concisus with use of a polycarbonate filter (n = 114) compared to a cellulose acetate filter (n = 79) (p < .0001). the polycarbonate filter is superior to the commonly used cellulose acetate filter for detection of c. concisus. | 2013 | 23743174 |
| production and evaluation of chicken egg-yolk-derived antibodies against campylobacter jejuni colonization-associated proteins. | campylobacter jejuni is one of the most important causes of foodborne gastroenteritis. chickens are considered a reservoir host of c. jejuni, and epidemiological studies have shown that contaminated chicken meat is a primary source of human infection. the objective of this study was to produce chicken egg-yolk-derived antibody (igy) against the five c. jejuni colonization-associated proteins or caps (cadf, flaa, momp, flpa, and cmec). recombinant c. jejuni caps were expressed in escherichia coli ... | 2013 | 23742296 |
| systemic use of fluoroquinolone in children. | fluoroquinolones are an important class of antibiotics that are widely used in adult patients because of their broad spectrum of activity, good tissue penetration, and oral bioavailability. however, fluoroquinolone use in children is limited because juvenile animals developed arthropathy in previous experiments on fluoroquinolone use. indications for fluoroquinolone use in patients younger than 18 years, as stated by the u.s. food and drug administration, include treatment of complicated urinary ... | 2013 | 23741232 |
| cell extract-containing medium for culture of intracellular fastidious bacteria. | the culture of fastidious microorganisms is a critical step in infectious disease studies. as a proof-of-concept experiment, we evaluated an empirical medium containing eukaryotic cell extracts for its ability to support the growth of coxiella burnetii. here, we demonstrate the exponential growth of several bacterial strains, including the c. burnetii nine mile phase i and phase ii strains, and c. burnetii isolates from humans and animals. low-oxygen-tension conditions and the presence of small ... | 2013 | 23740722 |
| incidence of listeria spp. in dairy cows feed and raw milk in latvia. | feed is a risk factor for poisoning the farm environment thus also fresh milk with pathogenic microorganisms of listeria genus species. listeria ivanovii, listeria innocua, and listeria seeligeri were isolated from 9.2%, but listeria monocytogenes from 20.0% of feed samples. most often different fodders (9.3%) and silage (4.7%) were contaminated with listeria monocytogenes. listeria genus species were isolated more often from feed prepared and used in organic dairy farm than from that used in co ... | 2012 | 23738125 |
| neonatal immune adaptation of the gut and its role during infections. | the intestinal tract is engaged in a relationship with a dense and complex microbial ecosystem, the microbiota. the establishment of this symbiosis is essential for host physiology, metabolism, and immune homeostasis. because newborns are essentially sterile, the first exposure to microorganisms and environmental endotoxins during the neonatal period is followed by a crucial sequence of active events leading to immune tolerance and homeostasis. contact with potent immunostimulatory molecules sta ... | 2013 | 23737810 |
| identification of a functional type vi secretion system in campylobacter jejuni conferring capsule polysaccharide sensitive cytotoxicity. | the pathogen campylobacter jejuni is the principal cause of bacterial food-borne infections. the mechanism(s) that contribute to bacterial survival and disease are still poorly understood. in other bacterial species, type vi secretion systems (t6ss) are increasingly recognized to contribute to bacterial pathogenesis by toxic effects on host cells or competing bacterial species. here we report the presence of a functional type vi secretion system in c. jejuni. proteome and genetic analyses reveal ... | 2013 | 23737749 |
| streptococcus pneumoniae serotype 11d has a bispecific glycosyltransferase and expresses two different capsular polysaccharide repeating units. | streptococcus pneumoniae (pneumococcus) expresses a capsular polysaccharide (cps) that protects against host immunity and is synthesized by enzymes in the capsular polysaccharide synthesis (cps) locus. serogroup 11 has six members (11a to -e) and the cps structure of all members has been solved, except for serotype 11d. the cps loci of 11a and 11d differ by one codon (n112s) in wcrl, which putatively encodes a glycosyltransferase that adds the fourth sugar of the cps repeating unit (ru). gas chr ... | 2013 | 23737526 |
| rapid identification of novel immunodominant proteins and characterization of a specific linear epitope of campylobacter jejuni. | campylobacter jejuni remains one of the major gut pathogens of our time. its zoonotic nature and wide-spread distribution in industrialized countries calls for a quick and reliable diagnostic tool. antibody-based detection presents a suitable means to identify pathogenic bacteria. however, the knowledge about immunodominant targets is limited. thus, an approach is presented, which allows for the rapid screening of numerous cdna derived expression clones to identify novel antigens. the deeper und ... | 2013 | 23734261 |
| elucidating the aetiology of human campylobacter coli infections. | there has been little research on the determinants of campylobacter coli infection, despite its contributing up to 10% of human campylobacter infections. a case-control and two case-case study methods explored the aetiology of c. coli over a one year period across scotland. the case-control multivariate model found an increased risk of c. coli infection in people older than 19 years (o.r. = 3.352), and during the summer months (o.r. = 2.596), while residing in an urban area decreased the risk (o ... | 2013 | 23734204 |
| genetic mechanisms of antimicrobial resistance identified in salmonella enterica, escherichia coli, and enteroccocus spp. isolated from u.s. food animals. | the prevalence of antimicrobial resistance (ar) in bacteria isolated from u.s. food animals has increased over the last several decades as have concerns of ar foodborne zoonotic human infections. resistance mechanisms identified in u.s. animal isolates of salmonella enterica included resistance to aminoglycosides (e.g., alleles of aacc, aada, aadb, ant, apha, and strab), β-lactams (e.g., bla cmy-2, tem-1, pse-1), chloramphenicol (e.g., flor, cmla, cat1, cat2), folate pathway inhibitors (e.g., al ... | 2013 | 23734150 |
| virulence of vancomycin-resistant enterococcus faecium according to linezolid resistance and clinical outbreak status. | assessing clinical virulence differences between vancomycin-resistant enterococcus faecium (vref) strains resistant to linezolid (lrvre) and linezolid-susceptible vre (lsvre) strains is difficult due to confounding patient variables. galleria mellonella is a validated host interaction model allowing straightforward organism virulence assessment. the objective of this study was to assess the virulence of vref in g. mellonella according to linezolid resistance and clinical outbreak status. a genet ... | 2013 | 23733458 |
| guillain-barre syndrome as a paraneoplastic manifestation of disseminated squamous cell carcinoma. | we describe a 65-year-old woman who developed ascending, symmetrical paraesthesia and weakness. this was on a background of metastatic disseminated squamous cell carcinoma, with a likely recurrent mandibular primary. serum testing for antiganglioside antibodies was strongly positive. despite a 5-day course of intravenous immunoglobulin, the patient passed away on day 34 of admission. this is the first case, to our knowledge, of guillain-barré syndrome in association with squamous cell carcimoma. | 2013 | 23729714 |
| allelic variation in a simple sequence repeat element of neisserial pglb2 and its consequences for protein expression and protein glycosylation. | neisseria species express an o-linked glycosylation system in which functionally distinct proteins are elaborated with variable glycans. a major source of glycan diversity in n. meningitidis results from two distinct pglb alleles responsible for the synthesis of either n,n'-diacetylbacillosamine or glyceramido-acetamido trideoxyhexose that occupy the reducing end of the oligosaccharides. alternative modifications at c-4 of the precursor udp-4-amino are attributable to distinct c-terminal domains ... | 2013 | 23729645 |
| pathogenic microbiological baseline survey of pork carcasses in taiwan. | from 2004 to 2010, pork carcass swabs from state-inspected slaughter plants in taiwan were intermittently analyzed to determine the prevalence of selected pathogenic microorganisms associated with foodborne illness. the prevalences of staphylococcus aureus each year from 2006 to 2010 were 6.6, 10.8, 5.1, 6.4, and 7.4%, respectively, while those of listeria monocytogenes were 1.2% in 2004, 1.3% in 2005, and 3.5% in 2008. the prevalences of clostridium perfringens were 0.9% in 2004, 3.2% in 2005, ... | 2013 | 23726203 |
| "thermal inactivation of campylobacter jejuni in broth," a comment on: j. food prot. 75(6):1029-1035 (2012). | 2013 | 23726185 | |
| the main aeromonas pathogenic factors. | the members of the aeromonas genus are ubiquitous, water-borne bacteria. they have been isolated from marine waters, rivers, lakes, swamps, sediments, chlorine water, water distribution systems, drinking water and residual waters; different types of food, such as meat, fish, seafood, vegetables, and processed foods. aeromonas strains are predominantly pathogenic to poikilothermic animals, and the mesophilic strains are emerging as important pathogens in humans, causing a variety of extraintestin ... | 2012 | 23724321 |
| cytomegalovirus colitis in a 10 year-old girl after kidney transplantation. | cytomegalovirus is an important infection in kidney transplantation. isolation of the cmv virus or detection of its proteins or nucleic acid in any body fluid or tissue specimen is defined as "cmv infection". | 2013 | 23724187 |
| expression, crystallization and preliminary crystallographic study of glub from corynebacterium glutamicum. | glub is a substrate-binding protein (sbp) which participates in the uptake of glutamic acid in corynebacterium glutamicum, a gram-positive bacterium. it is part of an atp-binding cassette (abc) transporter system. together with the transmembrane proteins gluc and glud and the cytoplasmic protein glua, which couples the hydrolysis of atp to the translocation of glutamate, they form a highly active glutamate-uptake system. as part of efforts to study the amino-acid metabolism, especially the metab ... | 2013 | 23722846 |
| a refined palate: bacterial consumption of host glycans in the gut. | the human intestine houses a dense microbial ecosystem in which the struggle for nutrients creates a continual and dynamic selective force. host-produced mucus glycans provide a ubiquitous source of carbon and energy for microbial species. not surprisingly, many gut resident bacteria have become highly adapted to efficiently consume numerous distinct structures present in host glycans. we propose that sophistication in mucus consumption is a trait most likely to be found in gut residents that ha ... | 2013 | 23720460 |
| development and application of a real-time polymerase chain reaction method for campylobacter jejuni detection. | to develop a real-time polymerase chain reaction (pcr) method to detect and quantify campylobacter jejuni (c. jejuni) from stool specimens. | 2013 | 23716989 |
| quorum-sensing autoinducers resuscitate dormant vibrio cholerae in environmental water samples. | cholera epidemics have long been known to spread through water contaminated with human fecal material containing the toxigenic bacterium vibrio cholerae. however, detection of v. cholerae in water is complicated by the existence of a dormant state in which the organism remains viable, but resists cultivation on routine bacteriological media. growth in the mammalian intestine has been reported to trigger "resuscitation" of such dormant cells, and these studies have prompted the search for resusci ... | 2013 | 23716683 |
| antibody to a conserved antigenic target is protective against diverse prokaryotic and eukaryotic pathogens. | microbial capsular antigens are effective vaccines but are chemically and immunologically diverse, resulting in a major barrier to their use against multiple pathogens. a β-(1→6)-linked poly-n-acetyl-d-glucosamine (pnag) surface capsule is synthesized by four proteins encoded in genetic loci designated intercellular adhesion in staphylococcus aureus or polyglucosamine in selected gram-negative bacterial pathogens. we report that many microbial pathogens lacking an identifiable intercellular adhe ... | 2013 | 23716675 |
| divergent mechanisms of interaction of helicobacter pylori and campylobacter jejuni with mucus and mucins. | helicobacter pylori and campylobacter jejuni colonize the stomach and intestinal mucus, respectively. using a combination of mucus-secreting cells, purified mucins, and a novel mucin microarray platform, we examined the interactions of these two organisms with mucus and mucins. h. pylori and c. jejuni bound to distinctly different mucins. c. jejuni displayed a striking tropism for chicken gastrointestinal mucins compared to mucins from other animals and preferentially bound mucins from specific ... | 2013 | 23716616 |
| escherichia coli heat-stable toxin b impairs intestinal epithelial barrier function by altering tight junction proteins. | escherichia coli heat-stable toxin b (stb) causes diarrhea in animals. stb binds to sulfatide, its receptor, and is then internalized. in the cytoplasm, through a cascade of events, stb triggers the opening of ion channels, allowing ion secretion and water loss and leading to diarrhea. tight junctions (tjs) are well known for controlling paracellular traffic of ions and water by forming a physical intercellular barrier in epithelial cells, and some bacterial toxins are known to affect adversely ... | 2013 | 23716609 |
| feob-mediated uptake of iron by francisella tularensis. | francisella tularensis, the bacterial cause of tularemia, infects the liver and replicates in hepatocytes in vivo and in vitro. however, the factors that govern adaptation of f. tularensis to the intrahepatocytic niche have not been identified. using cdna microarrays, we determined the transcriptional profile of the live vaccine strain (lvs) of f. tularensis grown in the fl83b murine hepatocytic cell line compared to that of f. tularensis cultured in broth. the fslc gene of the fsl operon was th ... | 2013 | 23716605 |
| bell's palsy presenting after a case of campylobacter enteritis. | a case of unilateral facial paralysis following infection with campylobacter jejuni is presented. | 2013 | 23713220 |
| alternative substrates selective for s-adenosylmethionine synthetases from pathogenic bacteria. | s-adenosyl-l-methionine (adomet) synthetase catalyzes the production of adomet, the major biological methyl donor and source of methylene, amino, ribosyl, and aminopropyl groups in the metabolism of all known organism. in addition to these essential functions, adomet can also serve as the precursor for two different families of quorum sensing molecules that trigger virulence in gram-negative human pathogenic bacteria. the enzyme responsible for adomet biosynthesis has been cloned, expressed and ... | 2013 | 23711747 |
| campylobacteriosis outbreak associated with consumption of undercooked chicken liver pâté in the east of england, september 2011: identification of a dose-response risk. | a foodborne outbreak with 49 cases (22 culture positive for campylobacter sp.) following a wedding party in the east of england was investigated. a retrospective cohort study identified an association between consumption of chicken liver pâté and infection with campylobacter jejuni/coli. there was a statistically significant association between dose (amount of chicken liver pâté eaten) and the risk of disease ['tasted': odds ratio (or) 1·5, 95% confidence interval (ci) 0·04-∞; 'partly eaten': or ... | 2013 | 23711104 |
| campylobacteriosis outbreak associated with consumption of undercooked chicken liver pâté in the east of england, september 2011: identification of a dose-response risk. | a foodborne outbreak with 49 cases (22 culture positive for campylobacter sp.) following a wedding party in the east of england was investigated. a retrospective cohort study identified an association between consumption of chicken liver pâté and infection with campylobacter jejuni/coli. there was a statistically significant association between dose (amount of chicken liver pâté eaten) and the risk of disease ['tasted': odds ratio (or) 1·5, 95% confidence interval (ci) 0·04-∞; 'partly eaten': or ... | 2013 | 23711104 |
| random and site-specific mutagenesis of the helicobacter pylori ferric uptake regulator provides insight into fur structure-function relationships. | the ferric uptake regulator (fur) of helicobacter pylori is a global regulator that is important for colonization and survival within the gastric mucosa. h. pylori fur is unique in its ability to activate and repress gene expression in both the iron-bound (fe-fur) and apo forms (apo-fur). in the current study we combined random and site-specific mutagenesis to identify amino acid residues important for both fe-fur and apo-fur function. we identified 25 mutations that affected fe-fur repression a ... | 2013 | 23710935 |
| igg subclass antibodies to human and bacterial hsp60 are not associated with disease activity and progression over time in axial spondyloarthritis. | spondyloarthritis (spa), an interrelated group of rheumatic diseases, has been suggested to be triggered by bacterial infections prior to the development of an autoimmune response that causes inflammation of the spinal and peripheral joints. because human heat shock protein 60 (hsp60), recently renamed hspd1, and bacterial hsp60 are highly homologous, immunological cross-reactivity has been proposed as a mechanism of disease initiation. however, previous investigations of the humoral immune resp ... | 2013 | 23705835 |
| genome sequences of the guillain-barre syndrome outbreak-associated campylobacter jejuni strains icdccj07002 and icdccj07004. | the first world-known and largest outbreak of 36 cases of guillain-barré syndrome caused by a preceding campylobacter jejuni infection was reported previously in china. during the outbreak, campylobacter jejuni strain icdccj07002 was isolated from a patient with persistent diarrhea for 21 days, and c. jejuni strain icdccj07004 was from a healthy carrier without any clinical symptoms at the same time. here, we report the draft genome sequence of strain icdccj07002 (1,698,407 bp, with a g+c conten ... | 2013 | 23704181 |
| campylobacter jejuni ferric-enterobactin receptor cfra is tonb3 dependent and mediates iron acquisition from structurally different catechol siderophores. | campylobacter jejuni nctc11168 does not produce any endogenous siderophores of its own yet requires the cfra enterobactin transporter for in vivo colonization. in addition, the genome of c. jejuni nctc11168 contains three distinct tonb energy transduction systems, named tonb1, tonb2, and tonb3, that have not been tested for their role in siderophore uptake or their functional redundancy. we demonstrate that c. jejuni nctc11168 transports ferric-enterobactin in an energy dependent manner that req ... | 2013 | 23702883 |
| the ferric uptake regulator of helicobacter pylori: a critical player in the battle for iron and colonization of the stomach. | helicobacter pylori is arguably one of the most successful pathogens; it colonizes the stomachs of more than half of the human population. colonization and persistence in such an inhospitable niche requires the presence of exquisite adaptive mechanisms. one of the proteins that contributes significantly to the remarkable adaptability of h. pylori is the ferric uptake regulator (fur), which functions as a master regulator of gene expression. in addition to genes directly related to iron homeostas ... | 2013 | 23701330 |
| a higher prevalence rate of campylobacter in retail beef livers compared to other beef and pork meat cuts. | the objectives of this study were to determine the prevalence of campylobacter jejuni and campylobacter coli in retail beef, beef livers, and pork meats purchased from the tulsa (ok, usa) area and to further characterize the isolates obtained through antimicrobial susceptibility testing. a total of 97 chilled retail beef (50 beef livers and 47 other cuts), and 100 pork samples were collected. the prevalence of campylobacter in beef livers was 39/50 (78%), while no campylobacter was isolated from ... | 2013 | 23698698 |
| real-time genomic epidemiological evaluation of human campylobacter isolates by use of whole-genome multilocus sequence typing. | sequence-based typing is essential for understanding the epidemiology of campylobacter infections, a major worldwide cause of bacterial gastroenteritis. we demonstrate the practical and rapid exploitation of whole-genome sequencing to provide routine definitive characterization of campylobacter jejuni and campylobacter coli for clinical and public health purposes. short-read data from 384 campylobacter clinical isolates collected over 4 months in oxford, united kingdom, were assembled de novo. c ... | 2013 | 23698529 |
| exploitation of bacterial n-linked glycosylation to develop a novel recombinant glycoconjugate vaccine against francisella tularensis. | glycoconjugate-based vaccines have proved to be effective at producing long-lasting protection against numerous pathogens. here, we describe the application of bacterial protein glycan coupling technology (pgct) to generate a novel recombinant glycoconjugate vaccine. we demonstrate the conjugation of the francisella tularensis o-antigen to the pseudomonas aeruginosa carrier protein exotoxin a using the campylobacter jejuni pglb oligosaccharyltransferase. the resultant recombinant f. tularensis g ... | 2013 | 23697804 |
| helicobacter pylori seropositivity and cognitive performance among us adults: evidence from a large national survey. | helicobacter pylori seropositivity is a potential risk for poor cognition among us adults. | 2013 | 23697465 |
| high-resolution transcriptome maps reveal strain-specific regulatory features of multiple campylobacter jejuni isolates. | campylobacter jejuni is currently the leading cause of bacterial gastroenteritis in humans. comparison of multiple campylobacter strains revealed a high genetic and phenotypic diversity. however, little is known about differences in transcriptome organization, gene expression, and small rna (srna) repertoires. here we present the first comparative primary transcriptome analysis based on the differential rna-seq (drna-seq) of four c. jejuni isolates. our approach includes a novel, generic method ... | 2013 | 23696746 |
| the polyphosphate kinase gene ppk2 is required for mycobacterium tuberculosis inorganic polyphosphate regulation and virulence. | the mycobacterium tuberculosis gene rv3232c/mt3329 (ppk2) encodes a class ii polyphosphate kinase, which hydrolyzes inorganic polyphosphate (poly p) to synthesize gtp. we assessed the role of ppk2 in m. tuberculosis poly p regulation, antibiotic tolerance, and virulence. a ppk2-deficient mutant (ppk2::tn) and its isogenic wild-type (wt) and complemented (comp) strains were studied. for each strain, the intrabacillary poly p content, mic of isoniazid, and growth kinetics during infection of j774 ... | 2013 | 23695835 |