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temporal genetic stability of stegomyia aegypti (= aedes aegypti) populations.the mosquito stegomyia aegypti (= aedes aegypti) (diptera: culicidae) is the primary vector of viruses that cause yellow fever, dengue and chikungunya fever. in the absence of effective vaccines, the reduction of these diseases relies on vector control strategies. the success of these strategies is tightly linked to the population dynamics of target populations. in the present study, 14 collections from st. aegypti populations separated by periods of 1-13 years were analysed to determine their t ...201626744174
empirical evidence of the effect of school gathering on the dynamics of dengue epidemics.dengue fever is an important vector-transmitted disease that affects more than 100 countries worldwide. locations where individuals tend to gather may play an important role in disease transmission in the presence of the vector. by controlling mosquitoes' breeding places, this study aims to analyze the effect of reducing transmission in elementary schools (grades 1-9) on the dynamics of the epidemic at a regional level.201626743450
development of a larvicidal nanoemulsion with pterodon emarginatus vogel oil.pterodon emarginatus vogel is a brazilian species that belongs to the family fabaceae, popularly known as sucupira. its oil has several biological activities, including potent larvicidal property against aedes aegypti. this insect is the vector of dengue, a tropical disease that has been considered a critical health problem in developing countries, such as brazil. most of dengue control methods involve larvicidal agents suspended or diluted in water and making active lipophilic natural products ...201626742099
detection of aedes aegypti, aedes albopictus, and aedes koreicus in the area of sochi, russia.following the identification of aedes (ae.) aegypti in the sochi area in russia at the beginning of 2000, entomological surveys were conducted during the summers of 2007, 2011, and 2012, leading to the identification of ae. albopictus and ae. koreicus. these findings highlight russia as being the only country in the world health organization european region with a documented presence of both ae. aegypti and ae. albopictus mosquitoes. both mosquito species are found on the coasts of the black sea ...201626741323
direct lateral maneuvers in hawkmoths.we used videography to investigate direct lateral maneuvers, i.e. 'sideslips', of the hawkmoth manduca sexta. m. sexta sideslip by rolling their entire body and wings to reorient their net force vector. during sideslip they increase net aerodynamic force by flapping with greater amplitude, (in both wing elevation and sweep), allowing them to continue to support body weight while rolled. to execute the roll maneuver we observed in sideslips, they use an asymmetric wing stroke; increasing the pitc ...201626740573
the neurotranscriptome of the aedes aegypti mosquito.a complete genome sequence and the advent of genome editing open up non-traditional model organisms to mechanistic genetic studies. the mosquito aedes aegypti is an important vector of infectious diseases such as dengue, chikungunya, and yellow fever and has a large and complex genome, which has slowed annotation efforts. we used comprehensive transcriptomic analysis of adult gene expression to improve the genome annotation and to provide a detailed tissue-specific catalogue of neural gene expre ...201626738925
bioactivity and laundering resistance of five commercially available, factory-treated permethrin-impregnated fabrics for the prevention of mosquito-borne diseases: the need for a standardized testing and licensing procedure.personal protective measures against hematophagous vectors constitute the first line of defense against arthropod-borne diseases. in this regard, a major advance has been the development of residual insecticides that can be impregnated into clothing. currently, however, information on specific treatment procedures, initial insecticide concentrations, arthropod toxicity, residual activity, and laundering resistance is either fragmentary or non-existent, and no world health organization pesticides ...201626738734
[identification of microrna-like viral small rnas from aaedv].several virus families have been shown to encode micrornas (mirnas), which have roles in the infection and replication of viruses in host cells. these virus-encoded mirnas are identified in double-stranded dna virus (dsdna virus) and in several rna virus families, but not in single-stranded dna virus (ssdna virus). we used a bioinformatics approach based on vmir, mirnafold and maturepred software to predict virus-encoded mirna-like small rnas from the genome of a ssdna virus: aedes aegypti denso ...201526738292
effects of argentilactone on the transcriptional profile, cell wall and oxidative stress of paracoccidioides spp.paracoccidioides spp., a dimorphic pathogenic fungus, is the etiologic agent of paracoccidioidomycosis (pcm). pcm is an endemic disease that affects at least 10 million people in latin america, causing severe public health problems. the drugs used against pathogenic fungi have various side effects and limited efficacy; therefore, there is an inevitable and urgent medical need for the development of new antifungal drugs. in the present study, we evaluated the transcriptional profile of paracoccid ...201626734764
the mutualistic side of wolbachia-isopod interactions: wolbachia mediated protection against pathogenic intracellular bacteria.wolbachia is a vertically transmitted endosymbiont whose radiative success is mainly related to various host reproductive manipulations that led to consider this symbiont as a conflictual reproductive parasite. however, lately, some wolbachia have been shown to act as beneficial symbionts by protecting hosts against a broad range of parasites. still, this protection has been mostly demonstrated in artificial wolbachia-host associations between partners that did not co-evolved together. here, we ...201526733946
identification of proteins bound to dengue viral rna in vivo reveals new host proteins important for virus replication.dengue virus is the most prevalent cause of arthropod-borne infection worldwide. due to the limited coding capacity of the viral genome and the complexity of the viral life cycle, host cell proteins play essential roles throughout the course of viral infection. host rna-binding proteins mediate various aspects of virus replication through their physical interactions with viral rna. here we describe a technique designed to identify such interactions in the context of infected cells using uv cross ...201626733069
the structure of a prophenoloxidase (ppo) from anopheles gambiae provides new insights into the mechanism of ppo activation.phenoloxidase (po)-catalyzed melanization is a universal defense mechanism of insects against pathogenic and parasitic infections. in mosquitos such as anopheles gambiae, melanotic encapsulation is a resistance mechanism against certain parasites that cause malaria and filariasis. po is initially synthesized by hemocytes and released into hemolymph as inactive prophenoloxidase (ppo), which is activated by a serine protease cascade upon recognition of foreign invaders. the mechanisms of ppo activ ...201626732497
species distribution modelling of aedes aegypti in two dengue-endemic regions of pakistan.statistical tools are effectively used to determine the distribution of mosquitoes and to make ecological inferences about the vector-borne disease dynamics. in this study, we utilised species distribution models to understand spatial patterns of aedes aegypti in two dengue-prevalent regions of pakistan, lahore and swat. species distribution models can potentially indicate the probability of suitability of ae. aegypti once introduced to new regions like swat, where invasion of this species is a ...201626729239
relevance of non-communicable comorbidities for the development of the severe forms of dengue: a systematic literature review.patients with dengue fever and comorbidities seem to be at higher risk of developing complications and/or severe dengue compared to healthier individuals. this study systematically reviews the evidence related to comorbidities and dengue. a systematic literature review was performed in five databases (embase, pubmed, global health, scielo, cochrane) and grey literature for full-text articles since its inceptions until october 10, 2015. a total of 230 articles were retrieved. sixteen studies were ...201626727113
a native wolbachia endosymbiont does not limit dengue virus infection in the mosquito aedes notoscriptus (diptera: culicidae).the endosymbiotic bacterium wolbachia pipientis infects many species of insects and has been transinfected into the mosquito aedes aegypti (l.), the primary vector of dengue virus (denv). recently, it has been shown that wolbachia blocks the replication and transmission of rna viruses, such as denv, in a number of mosquito species including ae. aegypti and aedes albopictus (skuse), which is naturally infected with wolbachia and considered a secondary vector for denv. the mosquito species aedes n ...201626721865
protein-protein interactions between a. aegypti midgut and dengue virus 2: two-hybrid screens using the midgut cdna library.dengue virus (denv) is principally transmitted by the aedes aegypti mosquito. to date, mosquito population control remains the key strategy for reducing the continuing spread of denv. the focus on the development of new vector control strategies through an understanding of the mosquito-virus relationship is essential, especially targeting the midgut, which is the first mosquito organ exposed to denv infection.201526719940
determination of insecticidal effect (lc50 and lc90) of organic fatty acids mixture (c8910+silicone) against aedes aegypti and aedes albopictus (diptera: culicidae).emerging and re-emerging vector-borne diseases such as chikungunya and dengue and associated aedes vectors are expanding their historical ranges; thus, there is a need for the development of novel insecticides for use in vector control programs. the mosquito toxicity of a novel insecticide and repellent consisting of medium-chain carbon fatty acids (c8910) was examined. determination of lc50 and lc90 was made against colony-reared aedes aegypti (l.) and aedes albopictus (skuse) using probit anal ...201626718717
targeting renal epithelial channels for the control of insect vectors.three small molecules were identified in high throughput screens that 1) block renal inward rectifier potassium (kir) channels of aedes aegypti expressed in hek cells and xenopus oocytes, 2) inhibit the secretion of kcl but not nacl in isolated malpighian tubules, and after injection into the hemolymph, 3) inhibit kcl excretion in vivo, and 4) render mosquitoes flightless or dead within 24h. some mosquitoes had swollen abdomens at death consistent with renal failure. vu625, the most potent and p ...201526716074
neem oil increases the efficiency of the entomopathogenic fungus metarhizium anisopliae for the control of aedes aegypti (diptera: culicidae) larvae.entomopathogenic fungi are potential candidates for use in integrated vector management and many isolates are compatible with synthetic and natural insecticides. neem oil was tested separately and in combination with the entomopathogenic fungus metarhizium anisopliae against larvae of the dengue vector aedes aegypti. our aim was to increase the effectiveness of the fungus for the control of larval mosquito populations.201526715150
fitness cost in field and laboratory aedes aegypti populations associated with resistance to the insecticide temephos.the continued use of chemical insecticides in the context of the national program of dengue control in brazil has generated a high selective pressure on the natural populations of aedes aegypti, leading to their resistance to these compounds in the field. fitness costs have been described as adaptive consequences of resistance. this study evaluated the biological and reproductive performance of a. aegypti strains and a field population resistant to temephos, the main larvicide used for controlli ...201526715037
forced egg retention induced by diethyl-phenylacetamide diminishes the fecundity and longevity of dengue vectors.oviposition is an important event in the life cycle of mosquito which is a decisive factor for the fate of its progeny. if oviposition is prevented, the life cycle of mosquito is disrupted and the population growth can be reduced. diethyl-phenylacetamide (depa) is an effective topical repellent against various haematophagous insects. the study reports the egg retention effect and subsequent lethal effect of depa on the gravid females of aedes aegypti and ae. albopictus mosquitoes.201526714511
first evidence of dengue virus infection in wild caught mosquitoes during an outbreak in assam, northeast india.dengue is one of the major public health problems worldwide, transmitted mainly by aedes aegypti and ae. albopictus mosquitoes. rapid urbanisation and industrialisation have led to an increase in vector population in northeastern states of india. in 2013, guwahati, the capital city of assam, india experienced an outbreak of dengue. this study was undertaken with an objective to determine infection rates of dengue viruses (denv) in both the established vectors present in this region.201526714508
evidence of vertical transmission and co-circulation of chikungunya and dengue viruses in field populations of aedes aegypti (l.) from guerrero, mexico.we report results of the entomo-virological surveillance system in aedes aegypti local populations performed by the ministry of health of guerrero.201626711697
dissecting japan's dengue outbreak in 2014.despite japan's temperate climate, a dengue outbreak occurred in tokyo for the first time in over 70 years in 2014. we dissected this dengue outbreak based on phylogenetic analysis, travel interconnectivity, and environmental drivers for dengue epidemics. comparing the available dengue virus 1 (denv1) e gene sequence from this outbreak with 3,282 unique denv1 sequences in national center for biotechnology information suggested that the denv might have been imported from china, indonesia, singapo ...201526711518
fitness of walbb wolbachia infection in aedes aegypti: parameter estimates in an outcrossed background and potential for population invasion.wolbachia endosymbionts are potentially useful tools for suppressing disease transmission by aedes aegypti mosquitoes because wolbachia can interfere with the transmission of dengue and other viruses as well as causing deleterious effects on their mosquito hosts. most recent research has focused on the wmel infection, but other infections also influence viral transmission and may spread in natural populations. here, we focus on the walbb infection in an australian outbred background and show tha ...201626711515
larvicidal activity of catechin isolated from leucas aspera against aedes aegypti, anopheles stephensi, and culex quinquefasciatus (diptera: culicidae).vector control is facing a threat due to the emergence of resistance to synthetic insecticides. insecticides of plant origin my serve as an alternative biocontrol technique in the future. the aim of the present study was to evaluate the larvicidal activity of fractions and compounds from the whole-plant methanol extracts of leucas aspera on the fourth-instar larvae of aedes aegypti, anopheles stephensi, and culex quinquefasciatus. the larvae were exposed to fractions with concentrations ranging ...201626711450
[zika virus: another emerging arbovirus in venezuela?].current health conditions of venezuela, together with the tropical proliferation of different types of aedes (aegypti and albopictus), suggest the arrival to this country of zika virus (zikv). the pan american health organization urges latin american countries to take measures to prevent the zikv entry and advises that these measures must be designed to detect the introduction of zikv in an area, track its spread and actively monitor the disease. in venezuela and other latin-american countries, ...201526710538
how reticulated are species?many groups of closely related species have reticulate phylogenies. recent genomic analyses are showing this in many insects and vertebrates, as well as in microbes and plants. in microbes, lateral gene transfer is the dominant process that spoils strictly tree-like phylogenies, but in multicellular eukaryotes hybridization and introgression among related species is probably more important. because many species, including the ancestors of ancient major lineages, seem to evolve rapidly in adaptiv ...201526709836
larvicidal activity, inhibition effect on development, histopathological alteration and morphological aberration induced by seaweed extracts in aedes aegypti (diptera: culicidae).to investigate the larvicidal activity, inhibition effect on development, histopathological alteration and morphological aberration induced by the extracts derived from seaweeds bryopsis pennata (b. pennata), sargassum binderi (s. binderi) and padina australis in aedes aegypti (ae. aegypti) larvae and to characterize the phytochemical components of the three seaweeds.201526706671
rna interference in colorado potato beetle: steps toward development of dsrna as a commercial insecticide.colorado potato beetle (cpb) is a notorious pest on potatoes and has a remarkable ability to detoxify plant chemicals and develop resistance against insecticides. dsrna targeting cpb genes could be expressed in potato plants to control this pest. however, previous attempts at introducing transgenic potato plants to control cpb were not highly successful. recent studies showed that feeding dsrna expressed in bacteria works very well to kill cpb. to realize the potential of rnai to control this an ...026705514
virus evolution and transmission in an ever more connected world.the frequency and global impact of infectious disease outbreaks, particularly those caused by emerging viruses, demonstrate the need for a better understanding of how spatial ecology and pathogen evolution jointly shape epidemic dynamics. advances in computational techniques and the increasing availability of genetic and geospatial data are helping to address this problem, particularly when both information sources are combined. here, we review research at the intersection of evolutionary biolog ...026702033
generation of recombinant oropouche viruses lacking the nonstructural protein nsm or nss.oropouche virus (orov) is a midge-borne human pathogen with a geographic distribution in south america. orov was first isolated in 1955, and since then, it has been known to cause recurring outbreaks of a dengue-like illness in the amazonian regions of brazil. orov, however, remains one of the most poorly understood emerging viral zoonoses. here we describe the successful recovery of infectious orov entirely from cdna copies of its genome and generation of orov mutant viruses lacking either the ...201526699638
predicting wolbachia potential to knock down dengue virus transmission.releasing mosquitoes infected with the intracellular bacteria wolbachia is a candidate strategy for dengue control that has recently advanced to field-testing. a critical next step is to evaluate the impact of this strategy on dengue epidemiology. a recent study by ferguson and colleagues presents a mathematical framework to predict the likely effect of mosquitoes carrying wolbachia on dengue virus transmission. fitting the mathematical model to empirical data obtained with wolbachia-infected mo ...026697448
combining rna-seq and proteomic profiling to identify seminal fluid proteins in the migratory grasshopper melanoplus sanguinipes (f).seminal fluid proteins control many aspects of fertilization and in turn, they play a key role in post-mating sexual selection and possibly reproductive isolation. because effective proteome profiling relies on the availability of high-quality dna reference databases, our knowledge of these proteins is still largely limited to model organisms with ample genetic resources. new advances in sequencing technology allow for the rapid characterization of transcriptomes at low cost. by combining high t ...201526694822
review of ten-years presence of aedes albopictus in spain 2004-2014: known distribution and public health concerns.ten years have gone by since the asian tiger mosquito aedes albopictus was recorded for the first time in spain. in this paper, all relevant published information about this vector in spain for the period 2004-2014 is reviewed. the known distribution for 2014 is provided, including all historical records (published and unpublished data) and the results from samplings of the last year. the consequences on public health about the presence of the asian tiger mosquito in spain are also highlighted. ...201526694818
different effects of bacillus thuringiensis toxin cry1ab on midgut cell transmembrane potential of mythimna separata and agrotis ipsilon larvae.bacillus thuringiensis (bt) cry toxins from the cry1a family demonstrate significantly different toxicities against members of the family noctuidae for unknown reasons. in this study, membrane potential was measured and analyzed in freshly isolated midgut samples from mythimna separata and agrotis ipsilon larvae under oral administration and in vitro incubation with bt toxin cry1ab to elucidate the mechanism of action for further control of these pests. bioassay results showed that the larvae of ...201526694463
development of a mosquito attractant blend of small molecules against host-seeking aedes aegypti.a mosquito's dependence on olfaction in the hunt for human host could be efficiently exploited to protect humans from mosquito bites. the present study is undertaken to make the most attractant compound blend for aedes aegypti mosquitoes to lure them to traps. eleven molecules (m1-m11) at different dilutions were screened for attractancy against non-blood-fed adult female mosquitoes in an olfactometer. the results showed that the attractancy was dependent on both the chemical nature of the molec ...201626693718
importance of thr(328) and thr(369) for functional maintenance of two receptor-binding β-hairpins of the bacillus thuringiensis cry4ba toxin: implications for synergistic interactions with cyt2aa2.bacillus thuringiensis cry4ba mosquito-active toxin was previously shown to utilize two critical loop-residues, tyr(332) and phe(364) which are respectively located in β2-β3 and β4-β5 loops, for synergistic interactions with its alternative receptor-cyt2aa2. here, structural analysis of the cry4ba-receptor-binding domain revealed that its n-terminal subdomain encompasses β2-β3 and β4-β5 hairpins which are stabilized by inter-hairpin hydrogen bonding between thr(328) in β2 and thr(369) in β5. fun ...201626692482
zika virus in brazil and the danger of infestation by aedes (stegomyia) mosquitoes.zika virus, already widely distributed in africa and asia, was recently reported in two northeastern brazilian: state of bahia and state of rio grande do norte, and one southeastern: state of são paulo. this finding adds a potentially noxious virus to a list of several other viruses that are widely transmitted by aedes (stegomyia) aegypti and aedes (stegomyia) albopictus in brazil. the pathology and epidemiology, including the distribution and vectors associated with zika virus, are reviewed. th ...201626689277
synthesis of silver nanoparticles using catharanthus roseus root extract and its larvicidal effects.phytosynthesis of silver nanoparticles has attracted considerable attention due to their biocompatibility, low toxicity, cost-effectiveness and being a novel method has an eco-friendly approach. biological activity of root extracts as well as synthesized silver nanoparticles of catharanthus roseus were evaluated against larvae of aedes aegyptiand culex quinquefasciatus. the structure and proportion of the synthesized nanoparticles was defined by exploitation ultraviolet spectrophotometry, x-ray ...201526688962
clip-domain serine proteases as immune factors in insect hemolymph.clip proteases are non-digestive serine proteases present in hemolymph of insects and other arthropods. they are composed of one or more amino-terminal clip domains followed by a linker sequence and a carboxyl-terminal s1a family serine protease domain. the genes for clip proteases have evolved as four clades (clipa, clipb, clipc, clipd), each present as multigene families in insect genomes. clip proteases in hemolymph function in innate immune responses. these include proteolytic activation of ...026688791
mosquito larvicidal activity of silver nanoparticles synthesised using actinobacterium, streptomyces sp. m25 against anopheles subpictus, culex quinquefasciatus and aedes aegypti.the present work reports the larvicidal potential of microbially synthesised silver nanoparticles (agnps) by using an actinobacterium, streptomyces sp. m25 isolated from western ghats, tamil nadu, india. the biomass of streptomyces sp. was exposed to 1 mm silver nitrate (agno3) solution. the synthesis of agnps was confirmed by visual inspection followed by instrumental analysis such as uv-vis spectroscopy, fourier transform infrared spectroscopy, x-ray diffraction (xrd), energy dispersive x-ray ...201326688633
molecular characterization of midgut microbiota of aedes albopictus and aedes aegypti from arunachal pradesh, india.microbiota inhabiting midguts of mosquitoes play a key role in the host - parasite interaction and enhance vectorial capacity of viral diseases like dengue and chikungunya fevers. mosquito midgut is considered to be an important site for host-pathogen interaction and pathogen survival is thought to be an outcome of this interaction. in the present study we examined the bacterial community in the midgut of aedes mosquitoes in arunanchal pradesh, india, a subtropical zone where dengue fever is rep ...201526684012
functional characterization of nadph-cytochrome p450 reductase from bactrocera dorsalis: possible involvement in susceptibility to malathion.nadph cytochrome p450 reductase (cpr) is essential for cytochrome p450 catalysis, which is important in the detoxification and activation of xenobiotics. in this study, two transcripts of bactrocera dorsalis cpr (bdcpr) were cloned, and the deduced amino-acid sequence had an n-terminus membrane anchor for bdcpr-x1 and three conserved binding domains (fmn, fad, and nadp), as well as an fad binding motif and catalytic residues for both bdcpr-x1 and bdcpr-x2. bdcpr-x1 was detected to have the high ...201526681597
effect of isodillapiole on the expression of the insecticide resistance genes gste7 and cyp6n12 in aedes aegypti from central amazonia.the yellow fever mosquito aedes (stegomyia) aegypti is the main vector of dengue arbovirus and other arboviruses. dengue prevention measures for the control of a. aegypti involve mainly the use of synthetic insecticides. the constant use of insecticides has caused resistance in this mosquito. alternative studies on plant extracts and their products have been conducted with the aim of controlling the spread of the mosquito. dillapiole is a compound found in essential oils of the plant piper adunc ...201526681019
assessment of blood collection from the lateral saphenous vein for microfilaria counts in mongolian gerbils (meriones unguiculatus) infected with brugia pahangi.the nih guidelines for survival bleeding of mice and rats note that using the retroorbital plexus has a greater potential for complications than do other methods of blood collection and that this procedure should be performed on anesthetized animals. lateral saphenous vein puncture has a low potential for complications and can be performed without anesthesia. mongolian gerbils (meriones unguiculatus) are the preferred rodent model for filarial parasite research. to monitor microfilaria counts in ...026678366
dissecting the role of e2 protein domains in alphavirus pathogenicity.alphaviruses represent a diverse set of arboviruses, many of which are important pathogens. chikungunya virus (chikv), an arthritis-inducing alphavirus, is the cause of a massive ongoing outbreak in the caribbean and south america. in contrast to chikv, other related alphaviruses, such as venezuelan equine encephalitis virus (veev) and semliki forest virus (sfv), can cause encephalitic disease. e2, the receptor binding protein, has been implicated as a determinant in cell tropism, host range, pa ...201526676771
aedes aegypti eggs oviposited on water surface collected from field ovitraps in nova iguaçu city, brazil.aedes aegypti eggs can be collected from the water surface.201526676505
a mosquito survey of the twin-island caribbean nation of saint kitts and nevis, 2010.adult mosquito surveys of saint kitts and nevis (skn) were performed in the dry season (march 16-23, 2010) in saint kitts, and the rainy season (october 18-25, 2010) in skn. biogents (bg) sentinel traps were set with co₂and bg lure in urban, rural, mangrove, and dry forest habitats. mosquitoes were identified to species, and reverse transcription-polymerase chain reaction was performed on potential vector species for dengue virus (denv), chikungunya virus (chikv), and west nile virus (wnv). the ...201526675458
suitability of mixing fluorescent dye in adulticides and its impact on droplet characteristics and pesticide efficacy.fluorescent dyes are commonly used to help visualize insecticidal droplets or to trace movement of insecticides; however, the effect these dyes have on the insecticide's efficacy and droplet characteristics is unknown. this study evaluated the effects of mixing uvitex ob fluorescent dye with 5 adulticides on their efficacy in a wind tunnel. efficacy was determined via droplet size characteristics, spray flux (active ingredient [ai] deposition), and female adult aedes aegypti mortality. fyfanon® ...201526675457
interspecific cross-mating between aedes aegypti and aedes albopictus laboratory strains: implication of population density on mating behaviors.mating behavior between aedes aegypti and ae. albopictus, established colony strains were examined under laboratory conditions (30-cm(3) screened cages) for 5 consecutive days. the effect of selected male densities (30, 20, 10) and female density (20) on the number of swarming, mating pairs, eggs produced, and inseminated females were evaluated. male densities significantly increased swarming behavior, mating pairs, and egg production of heterospecific females, but female insemination was reduce ...201526675452
intra-cellular bacterial infections affect learning and memory capacities of an invertebrate.how host manipulation by parasites evolves is fascinating but challenging evolutionary question remains. many parasites share the capacity to manipulate host behavior increasing their transmission success. however, little is known about the learning and memory impact of parasites on their host. wolbachia are widespread endosymbionts and infect most insect species. these bacteria are maternally transmitted and mainly alter the reproduction of hosts with weak virulence. we tested the impact of par ...201526675213
zika virus and the never-ending story of emerging pathogens and transfusion medicine.in the last few years, the transfusion medicine community has been paying special attention to emerging vector-borne diseases transmitted by arboviruses. zika virus is the latest of these pathogens and is responsible for major outbreaks in africa, asia and, more recently, in previously infection-naïve territories of the pacific area. many issues regarding this emerging pathogen remain unclear and require further investigation. national health authorities have adopted different prevention strateg ...201526674815
effect of quorum sensing by staphylococcus epidermidis on the attraction response of female adult yellow fever mosquitoes, aedes aegypti aegypti (linnaeus) (diptera: culicidae), to a blood-feeding source.aedes aegypti, the principal vector of yellow fever and dengue fever, is responsible for more than 30,000 deaths annually. compounds such as carbon dioxide, amino acids, fatty acids and other volatile organic compounds (vocs) have been widely studied for their role in attracting ae. aegypti to hosts. many vocs from humans are produced by associated skin microbiota. staphyloccocus epidermidis, although not the most abundant bacteria according to surveys of relative 16s ribosomal rna abundance, co ...201526674802
impact of insecticide resistance on the effectiveness of pyrethroid-based malaria vectors control tools in benin: decreased toxicity and repellent effect.since the first evidence of pyrethroids resistance in 1999 in benin, mutations have rapidly increased in mosquitoes and it is now difficult to design a study including a control area where malaria vectors are fully susceptible. few studies have assessed the after effect of resistance on the success of pyrethroid based prevention methods in mosquito populations. we therefore assessed the impact of resistance on the effectiveness of pyrethroids based indoor residual spraying (irs) in semi-field co ...201526674643
the role of octopamine receptor agonists in the synergistic toxicity of certain insect growth regulators (igrs) in controlling dengue vector aedes aegypti (diptera: culicidae) mosquito.the synergistic action of octopamine receptor agonists (or agonists) on many insecticide classes (e.g., organophosphorus, pyrethroids, and neonicotinoids) on aedes aegypti l. has been reported recently. an investigation of or agonist's effect on insect growth regulators (igrs) was undertaken to provide a better understanding of the mechanism of action. based on the igr bioassay, pyriproxyfen was the most potent igr insecticide tested (ec50=0.0019ng/ml). however, the lethal toxicity results indic ...201626672383
peridomestic infection as a determining factor of dengue transmission.the study of endemic dengue transmission is essential for proposing alternatives to impact its burden. the traditional paradigm establishes that transmission starts around cases, but there are few studies that determine the risk.201526671573
a white spot syndrome virus microrna promotes the virus infection by targeting the host stat.jak/stat pathway plays an important role in invertebrates during virus infection. however the microrna (mirna)-mediated regulation of jak/stat is not intensively investigated. viral mirnas, encoded by virus genome, have emerged as important regulators in the virus-host interactions. in this study, a wssv (white spot syndrome virus)-encoded mirna (wssv-mir-22) was characterized in shrimp during virus infection. the results showed that the viral mirna could promote wssv infection in shrimp by targ ...201526671453
some like it hot, but not too hot.a temperature-sensitive receptor prevents mosquitoes from being attracted to targets that are hotter than a potential host.201526670880
the cation channel trpa1 tunes mosquito thermotaxis to host temperatures.while most animals thermotax only to regulate their temperature, female mosquitoes are attracted to human body heat during pursuit of a blood meal. here we elucidate the basic rules of aedes aegypti thermotaxis and test the function of candidate thermoreceptors in this important behavior. we show that host-seeking mosquitoes are maximally attracted to thermal stimuli approximating host body temperatures, seeking relative warmth while avoiding both relative cool and stimuli exceeding host body te ...201526670734
investigation of chikungunya fever outbreak in laguna, philippines, 2012.in july 2012, the philippines national epidemiology center received a report of a suspected chikungunya fever outbreak in san pablo city, laguna province, the first chikungunya cases reported from the city since surveillance started in 2007. we conducted an outbreak investigation to identify risk factors associated with chikungunya.201526668759
the evolutionarily conserved tre2/bub2/cdc16 (tbc), lysin motif (lysm), domain catalytic (tldc) domain is neuroprotective against oxidative stress.oxidative stress is a pathological feature of many neurological disorders; therefore, utilizing proteins that are protective against such cellular insults is a potentially valuable therapeutic approach. oxidation resistance 1 (oxr1) has been shown previously to be critical for oxidative stress resistance in neuronal cells; deletion of this gene causes neurodegeneration in mice, yet conversely, overexpression of oxr1 is protective in cellular and mouse models of amyotrophic lateral sclerosis. how ...201526668325
off! clip-on repellent device with metofluthrin tested on aedes aegypti (diptera: culicidae) for mortality at different time intervals and distances.the off! clip-on mosquito-repellent device was tested outdoors against aedes aegypti (l.). a single treatment device was used against batches of caged adult, nonblood fed ae. aegypti at multiple locations 0.3m from treatment center. another set of cages was stationed 0.6m from treatment. a final set of cages was placed 0.9m away. trials ran for durations of 5, 15, 30, and 60 min. initial knockdown and mortality after 24 h was recorded. the devices had effective knockdown and mortality. this was ...201626668103
reproduction-immunity trade-offs in insects.immune defense and reproduction are physiologically and energetically demanding processes and have been observed to trade off in a diversity of female insects. increased reproductive effort results in reduced immunity, and reciprocally, infection and activation of the immune system reduce reproductive output. this trade-off can manifest at the physiological level (within an individual) and at the evolutionary level (genetic distinction among individuals in a population). the resource allocation ...201526667271
microsatellite loci cross-species transferability in aedes fluviatilis (diptera:culicidae): a cost-effective approach for population genetics studies.aedes fluviatilis is a neotropical mosquito species thought to be a potential vector of yellow fever viruses and can be infected with plasmodium gallinaceum in laboratory. a better understanding of its genetic structure is very important to understand its epidemiologic potential and how it is responding to urbanization. the objective of this study was to survey the transferability of microsatellites loci developed for other aedes to ae. fluviatilis.201526667177
nutrition-dependent control of insect development by insulin-like peptides.in metazoans, members of the insulin-like peptide (ilp) family play a role in multiple physiological functions in response to the nutritional status. ilps have been identified and characterized in a wide variety of insect species. insect ilps that are mainly produced by several pairs of medial neurosecretory cells in the brain circulate in the hemolymph and act systemically on target tissues. physiological and biochemical studies in lepidoptera and genetic studies in the fruit fly have greatly e ...026664828
aging in personal and social immunity: do immune traits senesce at the same rate?how much should an individual invest in immunity as it grows older? immunity is costly and its value is likely to change across an organism's lifespan. a limited number of studies have focused on how personal immune investment changes with age in insects, but we do not know how social immunity, immune responses that protect kin, changes across lifespan, or how resources are divided between these two arms of the immune response. in this study, both personal and social immune functions are conside ...201526664685
molecular modeling and docking study to elucidate novel chikungunya virus nsp2 protease inhibitors.chikungunya is one of the tropical viral infections that severely affect the asian and african countries. absence of any suitable drugs or vaccines against chikungunya virus till date makes it essential to identify and develop novel leads for the same. recently, nsp2 cysteine protease has been classified as a crucial drug target to combat infections caused by alphaviruses including chikungunya virus due to its involvement viral replication. here in, we investigated the structural aspects of the ...026664062
alkaline phosphatases are involved in the response of aedes aegypti larvae to intoxication with bacillus thuringiensis subsp. israelensis cry toxins.bacillus thuringiensis subsp. israelensis (bti) is a natural pathogen of dipterans widely used as a biological insecticide for mosquito control. to characterize the response of mosquitoes to intoxication with bti, the transcriptome profile of bti-exposed susceptible aedes aegypti larvae was analysed using illumina rna-seq. gene expression of 11 alkaline phosphatases (alps) was further investigated by real time quantitative polymerase chain reaction and alp activity was measured in the susceptibl ...201626663676
direct pcr of indigenous and invasive mosquito species: a time- and cost-effective technique of mosquito barcoding.millions of people die each year as a result of pathogens transmitted by mosquitoes. however, the morphological identification of mosquito species can be difficult even for experts. the identification of morphologically indistinguishable species, such as members of the anopheles maculipennis complex (diptera: culicidae), and possible hybrids, such as culex pipiens pipiens/culex pipiens molestus (diptera: culicidae), presents a major problem. in addition, the detection and discrimination of newly ...201526663040
three-month real-time dengue forecast models: an early warning system for outbreak alerts and policy decision support in singapore.with its tropical rainforest climate, rapid urbanization, and changing demography and ecology, singapore experiences endemic dengue; the last large outbreak in 2013 culminated in 22,170 cases. in the absence of a vaccine on the market, vector control is the key approach for prevention.201526662617
expression profiles and rnai silencing of inhibitor of apoptosis transcripts in aedes, anopheles, and culex mosquitoes (diptera: culicidae).effective mosquito control is vital to curtail the devastating health effects of many vectored diseases. rna interference (rnai)-mediated control of mosquitoes is an attractive alternative to conventional chemical pesticides. previous studies have suggested that transcripts for inhibitors of apoptosis (iaps) may be good rnai targets. to revisit and extend previous reports, we examined the expression of aedes aegypti (l.) iaps (aaeiaps) 1, 2, 5, 6, 9, and a viral iap-associated factor (viaf) as w ...201626659858
genome sequence of the mosquitocidal bacillus thuringiensis strain br58, a biopesticide product effective against the coffee berry borer (hypothenemus hampei).bacillus thuringiensis is an important microbial control agent against insect pests. the draft genome sequence of the brazilian strain br58 described here contains the insecticidal genes cry4a, cry4b, cry10a, cry11a, cry60a, cry60b, and cyt1a, which show toxicity to both aedes aegypti and hypothenemus hampei larvae.201526659669
spatial clustering of aedes aegypti (diptera: culicidae) and its impact on entomological surveillance indicators.spatial clustering of aedes aegypti (l.) was assessed in 0.25-km2 sampling plots located in three different neighborhoods in the city of rio de janeiro, brazil monitoring was carried out with two oviposition traps (collecting either. eggs or adult mosquitoes) over a consecutive 80-wk period. taylor power laws (tpl) were used to assess clustering in both adult and egg collections. the clustering level observed in both tpl models was sufficient to increase by two to three times the sampling effort ...201626659607
neurotropic virus infections as the cause of immediate and delayed neuropathology.a wide range of viruses from different virus families in different geographical areas, may cause immediate or delayed neuropathological changes and neurological manifestations in humans and animals. infection by neurotropic viruses as well as the resulting immune response can irreversibly disrupt the complex structural and functional architecture of the central nervous system, frequently leaving the patient or affected animal with a poor or fatal prognosis. mechanisms that govern neuropathogenes ...201526659576
potential applications of insect symbionts in biotechnology.symbiotic interactions between insects and microorganisms are widespread in nature and are often the source of ecological innovations. in addition to supplementing their host with essential nutrients, microbial symbionts can produce enzymes that help degrade their food source as well as small molecules that defend against pathogens, parasites, and predators. as such, the study of insect ecology and symbiosis represents an important source of chemical compounds and enzymes with potential biotechn ...201526659224
coevolution of the ile1,016 and cys1,534 mutations in the voltage gated sodium channel gene of aedes aegypti in mexico.worldwide the mosquito aedes aegypti (l.) is the principal urban vector of dengue viruses. currently 2.5 billion people are at risk for infection and reduction of ae. aegypti populations is the most effective means to reduce the risk of transmission. pyrethroids are used extensively for adult mosquito control, especially during dengue outbreaks. pyrethroids promote activation and prolong the activation of the voltage gated sodium channel protein (vgsc) by interacting with two distinct pyrethroid ...201526658798
the drosophila zinc finger transcription factor ouija board controls ecdysteroid biosynthesis through specific regulation of spookier.steroid hormones are crucial for many biological events in multicellular organisms. in insects, the principal steroid hormones are ecdysteroids, which play essential roles in regulating molting and metamorphosis. during larval and pupal development, ecdysteroids are synthesized in the prothoracic gland (pg) from dietary cholesterol via a series of hydroxylation and oxidation steps. the expression of all but one of the known ecdysteroid biosynthetic enzymes is restricted to the pg, but the transc ...201526658797
methoprene-tolerant (met) and krüpple-homologue 1 (kr-h1) are required for ovariole development and egg maturation in the brown plant hopper.the brown plant hopper is one of the most destructive known pests of rice. we studied the roles of the jh receptor met and the downstream transcription factor kr-h1 in ovariole development and egg maturation. the predicted met protein in n. lugens (nlmet) contained 517 amino acids. qrt-pcr showed that nlmet was expressed in all tissues and that the highest expression occurred in the embryonic stage. in nlmet- or nlkr-h1-silenced female adults, ovarian development varied significantly, whereas th ...201526658174
knockdown of a nutrient amino acid transporter gene ldnat1 reduces free neutral amino acid contents and impairs leptinotarsa decemlineata pupation.a leptinotarsa decemlineata slc6 nat gene (ldnat1) was cloned. ldnat1 was highly expressed in the larval alimentary canal especially midgut. ldnat1 mrna levels were high right after the molt and low just before the molt. jh and a jh analog pyriproxyfen activated ldnat1 expression. rnai of an allatostatin gene ldas-c increased jh and upregulated ldnat1 transcription. conversely, silencing of a jh biosynthesis gene ldjhamt decreased jh and reduced ldnat1 expression. moreover, 20e and an ecdysteroi ...201526657797
human social behavior and demography drive patterns of fine-scale dengue transmission in endemic areas of colombia.dengue is known to transmit between humans and a. aegypti mosquitoes living in neighboring houses. although transmission is thought to be highly heterogeneous in both space and time, little is known about the patterns and drivers of transmission in groups of houses in endemic settings. we carried out surveys of pcr positivity in children residing in 2-block patches of highly endemic cities of colombia. we found high levels of heterogeneity in pcr positivity, varying from less than 30% in 8 of th ...201526656072
enhanced performance of an innovative dengue igg/igm rapid diagnostic test using an anti-dengue edi monoclonal antibody and dengue virus antigen.high levels of anti-dengue igm or igg can be detected using numerous rapid diagnostic tests (rdts). however, the sensitivity and specificity of these tests are reduced by changes in envelope glycoprotein antigenicity that inevitably occur in limited expression systems. a novel rdt was designed to enhance diagnostic sensitivity. dengue viruses cultured in animal cells were used as antigens to retain the native viral coat protein. monoclonal antibodies (mabs) were then developed, for the first tim ...201526655854
only a minority of broad-range detoxification genes respond to a variety of phytotoxins in generalist bemisia tabaci species.generalist insect can utilize two different modes for regulating their detoxification genes, the constitutive mode and the induced mode. here, we used the bemisia tabaci sibling species meam1 and med, as a model system for studying constitutive and induced detoxification resistance and their associated tradeoffs. b. tabaci adults were allowed to feed through membranes for 24 h on diet containing only sucrose or sucrose with various phytotoxins. quantitative real-time pcr analyses of 18 detoxific ...201526655836
nmr structure and function of helicoverpa armigera sterol carrier protein-2, an important insecticidal target from the cotton bollworm.the cotton bollworm, helicoverpa armigera, has developed strong resistance to many insecticides. sterol carrier protein-2 (scp-2) is an important non-specific lipid transfer protein in insects and appears to be a potential new target. in order to elucidate the structure and function of helicoverpa armigera scp-2 (hascp-2), nmr spectroscopy, docking simulations, mutagenesis and bioassays were performed. hascp-2 composed of five α-helices and four stranded β-sheets. the folds of α-helices and β-sh ...201526655641
genome-wide identification and expression profiling of serine proteases and homologs in the diamondback moth, plutella xylostella (l.).serine proteases (sps) are crucial proteolytic enzymes responsible for digestion and other processes including signal transduction and immune responses in insects. serine protease homologs (sphs) lack catalytic activity but are involved in innate immunity. this study presents a genome-wide investigation of sps and sphs in the diamondback moth, plutella xylostella (l.), a globally-distributed destructive pest of cruciferous crops.201526653876
administration of ivermectin to peridomestic cattle: a promising approach to target the residual transmission of human malaria.the success of current control tools in combatting malaria vectors is well established. however, sustained residual transmission of plasmodium parasites persists. mass drug administration (mda) to humans of the endectocide ivermectin for vector control is receiving increasing attention. however, vectors feeding upon animals escape this promising approach. zoophagy of mosquitoes sustains both the vector population and endemic population of vector-borne pathogens. therefore, only a strategy that w ...201526651335
(e)-caryophyllene and α-humulene: aedes aegypti oviposition deterrents elucidated by gas chromatography-electrophysiological assay of commiphora leptophloeos leaf oil.aedes aegypti is responsible for the transmission of dengue, a disease that infects millions of people each year. although essential oils are well recognized as sources of compounds with repellent and larvicidal activities against the dengue mosquito, much less is known about their oviposition deterrent effects. commiphora leptophloeos, a tree native to south america, has important pharmacological properties, but the chemical profile and applicability of its essential oil in controlling the spre ...201526650757
accelerated evolution of constraint elements for hematophagic adaptation in mosquitoes.comparative genomics is a powerful approach that comprehensively interprets the genome. herein, we performed whole genome comparative analysis of 16 diptera genomes, including four mosquitoes and 12 drosophilae. we found more than 540 000 constraint elements (ces) in the diptera genome, with the majority found in the intergenic, coding and intronic regions. accelerated elements (aes) identified in mosquitoes were mostly in the protein-coding regions (>93%), which differs from vertebrates in geno ...026646568
estimating dengue type reproduction numbers for two provinces of sri lanka during the period 2013-14.dengue is an endemic disease in the southeast asian country sri lanka. two seasonal peaks of dengue incidence were observed every year since 2002 onwards. in this study, we formulate a 2-strain dengue model for analyzing the monthly seasonal dengue incidence data from 2 provinces of sri lanka during the period april 2013 to september 2014. the seasonality is incorporated in the model in terms of mosquito biting rate, which we assume to be time periodic. we estimated 2 primary reproduction number ...201526646355
transcriptional cellular responses in midgut tissue of aedes aegypti larvae following intoxication with cry11aa toxin from bacillus thuringiensis.although much is known about the mechanism of action of bacillus thuringiensis cry toxins, the target tissue cellular responses to toxin activity is less understood. previous transcriptomic studies indicated that significant changes in gene expression occurred during intoxication. however, most of these studies were done in organisms without a sequenced and annotated reference genome. a reference genome and transcriptome is available for the mosquito aedes aegypti, and its importance as a diseas ...201526645277
nutritional control of insect reproduction.the amino acid-target of rapamycin (aa/tor) and insulin pathways play a pivotal role in reproduction of female insects, serving as regulatory checkpoints that guarantee the sufficiency of nutrients for developing eggs. being evolutionary older, the aa/tor pathway functions as an initial nutritional sensor that not only activates nutritional responses in a tissue-specific manner, but is also involved in the control of insect insulin-like peptides (ilps) secretion. insulin and aa/tor pathways also ...026644995
the ancestral gene repertoire of animal stem cells.stem cells are pivotal for development and tissue homeostasis of multicellular animals, and the quest for a gene toolkit associated with the emergence of stem cells in a common ancestor of all metazoans remains a major challenge for evolutionary biology. we reconstructed the conserved gene repertoire of animal stem cells by transcriptomic profiling of totipotent archeocytes in the demosponge ephydatia fluviatilis and by tracing shared molecular signatures with flatworm and hydra stem cells. phyl ...201526644562
seroprevalence of asian lineage chikungunya virus infection on saint martin island, 7 months after the 2013 emergence.at the end of 2013, chikungunya virus (chikv) emerged in saint martin island, caribbean. the asian lineage was identified. seven months after this introduction, the seroprevalence was 16.9% in the population of saint martin and 39.0% of infections remained asymptomatic. this moderate attack rate and the apparent limited size of the outbreak in saint martin could be explained by control measures involved to lower the exposure of the inhabitants. other drivers such as climatic factors and populati ...201526643536
clinical and epidemiological characterization of the first recognized outbreak of dengue virus-type 2 in mozambique, 2014.since the first reported epidemic of dengue in pemba, the capital of cabo delgado province, in 1984-1985, no further cases have been reported in mozambique. in march 2014, the provincial health directorate of cabo delgado reported a suspected dengue outbreak in pemba, associated with a recent increase in the frequency of patients with nonmalarial febrile illness. an investigation conducted between march and june detected a total of 193 clinically suspected dengue patients in pemba and nampula, t ...201626643534
molecular differentiation of the african yellow fever vector aedes bromeliae (diptera: culicidae) from its sympatric non-vector sister species, aedes lilii.yellow fever continues to be a problem in sub-saharan africa with repeated epidemics occurring. the mosquito aedes bromeliae is a major vector of yellow fever, but it cannot be readily differentiated from its non-vector zoophilic sister species ae. lilii using morphological characters. genetic differences have been reported between anthropophilic ae. bromeliae and zoophilic ae. lilii and between forest and domestic populations. however, due to the application of different molecular markers and n ...201526641858
cloning and functional characterization of two btb genes in the predatory mite metaseiulus occidentalis.proteins containing the btb (bric-à-brac, tramtrack, and broad complex) domain typically share low sequence similarities and are involved in a wide range of cellular functions. we previously identified two putative and closely related btb genes, btb1 and btb2, in the genome of the predatory mite metaseiulus occidentalis. in the current study, full-length btb1 and btb2 cdnas were cloned and sequenced. btb1 and btb2 encode proteins of 380 and 401 amino acids, respectively. btb1 and btb2 proteins e ...201526640898
dengue: what it is and why there is more.in 2014, china experienced the worst outbreak of dengue fever in the last decade with over 40,000 dengue cases including six deaths by the end of october. as one of the "neglected" tropical diseases, dengue is affecting substantially increasing number of people and proportion of global population due to factors including globalization, human settlement, and possibly climate change. here, the authors summarized the most recent data about dengue outbreaks in china and reviewed the global trend of ...201526640300
oviposition behavior in aedes aegypti and aedes albopictus (diptera: culicidae) in response to the presence of heterospecific and conspecific larvae.in mosquitoes, location of suitable sites for oviposition requires a set of visual, tactile, and olfactory cues that influences females before laying their eggs. the ability of gravid females to distinguish among potential oviposition sites that will or will not support the growth, development, and survival of their progeny is critical. aedes aegypti (l.) and aedes albopictus (skuse) share ecological niches, being highly competitive in larval stage. we studied the oviposition behavior of both sp ...201626634825
arbovirus infections.arbovirus (arthropod-borne virus) infections are increasingly important causes of neurologic disease in the united states through both endemic transmission and travel-associated infections. this article reviews the major arbovirus infections that can cause neurologic disease likely to be encountered in the united states.026633778
a venom gland extracellular chitin-binding-like protein from pupal endoparasitoid wasps, pteromalus puparum, selectively binds chitin.chitin-binding proteins (cbps) are present in many species and they act in a variety of biological processes. we analyzed a pteromalus puparum venom apparatus proteome and transcriptome and identified a partial gene encoding a possible cbp. here, we report cloning a full-length cdna of a sequence encoding a chitin-binding-like protein (ppcbp) from p. puparum, a pupal endoparasitoid of pieris rapae. the cdna encoded a 96-amino-acid protein, including a secretory signal peptide and a chitin-bindin ...201526633500
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