Publications

diet-hormone interrelationships in the rat.	twenty-eight-day old male rats were fed, either ad libitum or in restricted amounts, isoenergetic diets containing 2, 5, 10, 15, 25, or 50% lactalbumin and 5, 11.9, or 21.1% fat for 8 weeks. they were then killed and the plasma levels of triiodothyronine (t3), thyroxine (t4), insulin, glucagon, corticosterone, norepinephrine (ne), and epinephrine (e) were measured. dietary changes explained most of the variation in the plasma concentrations of t3, t4, insulin, and glucagon but less than 20% of t ...	1978	712420
specific weight loss in hyperthyroidism.	to assess metabolic turnover in patients with hyperthyroidism weight loss in mg . kg-1 . min-1 (specific weight loss, swl) was measured by means of a new type of scale working on the buoyance principle with a minimal susceptibility of 10 mg per 100 kg. weight loss rate was significantly increased in hyperthyroid patients (n=22;swl, 22.0 +/-1.4 (se) mg. kg-1 . min-1) compared to health controls (n=11; swl, 10.0 +/-0.4 (se) mg . kg-1 . min-1). the fall in swl paralleled the decrease in thyroid hor ...	1978	711136
[bilateral carcinoma of the breast after radiotherapy and chemotherapy for hodgkin's disease (author's transl)].	a 34-year-old patient had radiotherapy and chemotherapy for hodgkin's disease of the nodular sclerosing type 2 a thirteen years prior to the treatment of a right cancer of the breast in the stage m0 n1 t3. seven months later the patient had a left cancer of the breast in the stage m0 n0 t4. the literature is reviewed and the possibility of occurrance of secondary carcinoma following treatment of hodgkin's disease is discussed.	1978	710884
[changes in serum thyrotropin, thyroxine and triiodothyronine after complete thyroidectomy (author's transl)].	changes in serum tsh, t4 and t3 concentrations after complete thyroidectomy were observed for 4 weeks in 9 euthyroid patients with thyroid carcinoma. completeness of the thyroidectomy was confirmed by a 131 i scintigram performed one or two months after the operation. serum tsh levels rose progressively during the 4 weeks of observation, and a significant increase was noticed as early as 3 days after the thyroidectomy. serum t4 and t3 concentrations decreased significantly 3 days and 18 hrs, res ...	1978	710646
hormonal changes in serum in young men during prolonged physical strain.	the endocrine response to severe physical strain including lack of sleep has been investigated in army personnel during a combat course of 5 days' duration. the thyroxine (t4) concentration in serum increased during the first 24 h, and then declined at a rate corresponding to a halflife of 7.6 days and on day 6 had reached the lowest level, 55 ng/ml. triiodothyronine (t3) displayed a similar pattern, although an increase during the first 24 h could not be demonstrated. within 48 h after the cour ...	1978	710393
serum thyroid hormones changes in patients undergoing caesarean section under general or regional anaesthesia.	the effects of anaesthesia on serum thyroid hormones were studied in 32 pregnant young women undergoing caesarean section at term. eighteen patients received general anaesthesia and 14 lumbar extradural blockade. maternal serum concentrations of thyrotrophin (tsh), thyroxine (t4), triiodothyronine (t3) and reverse triiodothyronine (rt3) were measured using radioimmunoassay at 0, delivery and 24 h. there were no significant changes in tsh in the two groups. t4 concentrations decreased significant ...	1978	708547
surgical treatment of advanced carcinomas of the base of the tongue.	during the decade of 1964 through 1973, thirty-four patients with advanced squamous carcinoma of the base of the tongue (20 with t3 lesions and 14 with t4 lesions) were treated by surgical resection. these patients underwent an operative procedure as the only and definitive form of treatment. twenty-eight (82.3 per cent) presented with clinically positive cervical nodal metastases (4 staged n1, 13 staged n2, and 11 staged n3). the number of primary lesions controlled, cervical metastasis, and th ...	1978	707732
ultrastructural identification of afferent fibers of cardiac origin in thoracic sympathetic nerves in the dog.	while cardiac afferent nerve activity has been recorded from the ventrolateral (vlcn) and ventromedial (vmcn) cardiac nerves, left dorsal and ventral ansae subclaviae, and left upper thoracic white rami communicantes, little anatomical evidence for the existence of afferent fibers in these nerves has been reported. this study was designed to characterize the normal ultrastructure of the above nerves and to identify afferent fibers in them through wallerian degeneration produced by dorsal root ga ...	1978	707315
radioimmunoassay of triiodothyronine and thyroxine in capillary blood.	a radioimmunoassay technique for the estimation of t3 and t4 levels in capillary blood is described and evaluated in comparison to a simultaneously drawn and equally analyzed sample from the cubital vein. a total number of 236 samples thus received from the capillary and the vein from euthyroid, hypo- and hyper-thyroid patients were collected. for both t3 and t4 a good correlation (r = 0.94) was obtained. the clinical implication would especially be for paediatric practice.	1978	705244
optimum laboratory test combinations for thyroid function studies, selected by discriminant analysis.	in 430 patients with a variety of thyroid disorders, linear discriminant analysis was used to select laboratory test combinations giving optimum diagnostic efficiency in thyroid function studies. unexpectedly, tsh was found valuable in the diagnosis of hyperthyroidism and t3 in hypothyroidism. these test combinations were found optimal for the separation of euthyroidism/hyperthyroidism: t3, tsh and t4; euthyroidism/hypothyroidism: combined free thyroid hormone index (fti) and tsh; and hyperthyro ...	1978	705229
[fundamental and clinical studies of radioimmunoassay for t4 ria (peg) kit (author's transl)].		1978	702935
absence of phospholipase activity in bacteriophage t4.	we assayed phospholipase activity in t4dt+ and in t mutant phage grown under permissive and restrictive conditions. there was no correlation between the presence of the t+ gene product and phospholipase activity. phospholipase activity in phage lysates could be attributed to the presence of bacterial debris or to the use of commercial dnase which contains phospholipase.	1978	702644
gene expression and stability of mrna affected by dna-arrested synthesis in gene 59, 46, and 47 mutants of bacteriophage t4.	the effect of bacteriophage t4 gene 59 mutations (dna-arrested synthesis) on kinetics of dna synthesis, gene expression, and stability of mrna has been studied. when escherichia coli b was infected by a t4 gene 59 mutant, dna synthesis proceeded to increase linearly after initiation, but started to decrease at 8 min and was completely arrested at 12 min at 37 degrees c. at various incubation temperatures (20 to 42 degrees c), the initial rates and times of arrest of dna synthesis were different, ...	1978	702642
specificity of initial synaptic contacts made on guinea-pig superior cervical ganglion cells during regeneration of the cervical sympathetic trunk.	1. largely appropriate synaptic connexions are formed with neurones in the superior cervical ganglion at long intervals after interruption of the preganglionic nerve. in the present study we have assessed the accuracy of connexions during the early stages of re-innervation by observing end-organ responses to ventral root stimulation in vivo, and by recording intracellularly from ganglion cells during ventral root stimulation in isolated preparations. 2. appropriate, but weak, end-organ responses ...	1978	702401
effects of exercise, altitude, and food on blood hormone and metabolite levels.	measurements of blood glucose and plasma free fatty acids (ffa), thyroxine (t4), free thyroxine (ft4), triiodothyronine (t3), and thyroid-stimulating hormone (tsh) were made on samples taken from fed and fasted human subjects while at rest and immediately after 20 min moderate exercise. six subjects were studied on successive weeks before (la1), during (ha1, ha2, ha3), and after (la2) a 3-wk sojourn at high altitude (3,650 m). the subjects and location were the same as those used for the energy ...	1978	701119
dna polymerase of phage t4 antimutator mutant cb121. a defective zinc metalloenzyme?		1978	700112
plasma triiodothyronine, thyroxine and thyrotrophin levels in germfree rats.	plasma t3, t4 and tsh levels in developing germfree rats were high, low and normal as compared with those in conventional counterparts. the high t3/t4 ratio in germfree rat plasma was lowered by cholestyramine feeding.	1978	700040
carcinoma of the vocal cord: results of treatment and time-dose relationships.	a retrospective analysis has been done of 173 patients with epidermoid carcinoma of the vocal cord treated with radiation therapy at rush-presbyterian-st. luke's medical center between january 1970 and december )974. one hundred fifty-eight patients (91.3%) were early (t1, t2) glottic lesion and 10 were advanced (t3, t4) lesion. five (2.9%) were presented with positive cervical lymph nodes initially. of 158 patients who had t1, t2n0m0 glottic tumor, 146 had definitive radiation therapy and 12 ha ...	1978	698909
a study of thyroid function in psychiatric in-patients.	the prevalence of abnormal serum total thyroxine (t4) and triiodothyronine (t3) concentrations were determined in 1,206 in-patients in two associated psychiatric hospitals. the biochemical pattern of primary hypothyroidism occurred in five females and one male (prevalence 0.5 per cent), but in only one patient was the diagnosis clinically obvious. eight patients (all female) were clinically hyperthyroid (prevalence 0.7 per cent), of whom six were previously undiagnosed. there was no evidence tha ...	1978	698489
bacteriophage t4 tail length is controlled by its baseplate.		1978	697770
[neonatal detection of hypothyroidism in the south-pyrenean region. results of 14,000 determinations of t4 in blood eluates collected on blotting-paper].	screening for neonatal hypothyroidism has been undertaken in southern france by the estimation of t4 in the eluates of blood spots on filter paper. since march 1976, 14,000 estimations have been made and four cases of hypothyroidism with very low levels of t4 have been detected (less than 38 pg/2 spots). two cases had high tsh levels (greater than 200 microunits/2 spots). blood was taken from the hypothyroid babies on the 20th day of life, to check the findings and in all cases the thyroxine was ...	1978	697532
thyroid function in hepatic schistosomiasis.	serum thyroxine (t4), serum tri-iodothyronine (t3-uptake) and the free thyroxine index in 17 men suffering from hepatosplenic schistosomiasis did not differ significantly from those of ten healthy male controls. there was no correlation between these values and serum protein levels or liver function tests.	1978	697441
[the distribution of t4-igg bacteriophage on the surface of red blood cell obtained from the patient with acquired hemolytic anemia whose anti-globulin test was negative by direct and positive by indirect method (author's transl)].		1978	696193
urinary excretion of thyroxine, triiodothyronine, 3,3',5'-triiodothyronine (reverse t3) and renal function in human newborns.	the urinary excretion and serum levels of thyroxine (t4), triiodothyronine (t3) and 3,3',5'-triiodothyronine (reverse t3) was estimated in a longitudinal study of human newborns. the maternal and cord blood was also studied. neonatal renal function was evaluated using endogenous creatinine clearance. in cord blood serum t3 was found to be lower than in maternal blood, but reverse t3 highly elevated. during the first 5 days of life serum t4 and t3 increased with maximum at 48 and 24 h in contrast ...	1978	696180
the thyroglobulin variations during the thyroid surgery for polynodular goiter, hyperthyroidism and cancer.	a radioimmunoassay (ria) double antibody system for human thyroglobulin (tgl) was developed with a sensitivity of 2.5 ng/ml t3 and t4 did not interfere in the antibody tgl binding. the blood was collected pre-, intra- and 24 hrs post-thyroidectomy from 50 females and 4 males diagnosed as polynodular goiter, hyperthyroidism, chronic thyroiditis and thyroid cancer. the ratios of the intra- and post-operatory values versus pre-operatory values were calculated. the basal values in the patients havin ...	1978	694400
experimental studies concerning the mechanism of the thyroid-pineal interrelationship. i. the action of the pineal peptides on the thyroid gland.	subcutaneous administration of a pineal polypeptide extract, "crinofizin", to adult male wistar rats having the thyroid function inhibited by prolonged administration of mtu, has important effects on both the whole organism (slightly correcting the weight increase rate, loweri g cholesterolemia) and the thyroid gland (reducing thyroid hyperplasia and playing a part in various stages of thyroid hormones metabolism). thus under pineal treatment one can observe a clear tendency to return to the nor ...	1978	694396
3'-terminal labelling of rna with t4 rna ligase.		1978	692735
the effect of blood infusion upon endurance capacity and ratings of perceived exertion.	the purpose of this investigation was to study the effect of blood infusion upon maximal endurance capacity and ratings of perceived exertaion (rpe). sixteen long-distance runners, 13 of whom were marathoners, undertook four trials (t1-t4) of a treadmill run to exhaustion over a five week period. criterion measures were time to exhaustion (te) and rpe during each t. data on hb, hct and rbcs were collected prior to each trial. based on te at t1, ss were matched and assigned to either the experime ...	1978	692300
isolation and characterization of bacteriophage t4 mutant preheads.	to determine the function of individual gene products in the assembly and maturation of the t4 prehead, we have isolated and characterized aberrant preheads produced by mutations in three of the t4 head genes. mutants in gene 21, which codes for the t4 maturation proteases, produce rather stable preheads whose morphology and protein composition are consistent with a wild-type prehead blocked in the maturation cleavages. mutants in gene 24 produce similar structures which are unstable because the ...	1978	691116
new late gene, dar, involved in the replication of bacteriophage t4 dna. iii. dna replicative intermediates of t4 dar and a gene 59 mutant suppressed by dar.	a mutation in the dar gene of phage t4 restored the arrested dna synthesis caused by the gene 59 mutation. we have studied the dna replicative intermediates in cells infected with a dar mutant and a dar-amc5 (gene 59) mutant by velocity sedimentation in neutral and alkaline sucrose gradients. in t4 dar-infected cells, compared to the wild type, three kinds of abnormalities were observed in dna replication (i) there were unusually rapidly sedimenting intermediates (800s). (ii) when centrifuged i ...	1978	691106
assembly of the scaffolding core of bacteriophage t4 preheads.		1978	691063
specificity of partial exclusion of bacteriophage t2 in crosses with t4.	an attempt was made to isolate a bacteriophage t4 mutant generally disturbed in its ability to exclude the localized exclusion sensitivity determinants of t2 from the progeny of crosses. the method used was a modification of that used previously, which revealed t4 mutants unable to exclude more than one of the t2 sensitive sites specifically. one out of 1000 isolates from a mutagenized t4 am56:am32 stock was found to be mutated in its exclusion properties. however, the mutant was not generally e ...	1978	690619
[experimental and clinical evaluations of t4 radioimmunoassay kit by a solid phase method--konsul t4 ria].		1978	688679
[determination of t4 with seralute-t4 ria kit].		1978	688639
the structural organization of dna packaged within the heads of t4 wild-type, isometric and giant bacteriophages.	we present electron microscopic and x-ray diffraction evidence concerning the structural organization of condensed dna within a series of t4 bacteriophage with the following head morphologies: prolate (wild-type), isometric and giant (with greatly increased axial ratio). in all cases, the dna helix segments are locally parallel and 27 a apart. for the giant particles, we show that the dna forms a large coil whose axis is perpendicular to the axis of the phage tail. this evidence, combined with p ...	1978	688382
[interaction between the hypothalamo-hypophyseal system and the thyroid gland during different age periods].	age peculiarities of interaction of the hypothalamo-hypophysial system and the thyroid gland with consideration to direct and feed-back control were studied. significant alterations appear in old age in the hypothalamo-hypophysial system and the thyroid gland at the level of direct and feed-back control; there is an increase of sensitivity of the thyroid gland tissue to tsh, and of the hypothalamus-hypophysis--to the t4 effect. this rise of sensitivity is largely determined by the activation of ...	1978	687803
t4 polynucleotide ligase catalyzed joining on triple-stranded nucleic acids.	dt1o will form triple-stranded complexes with dan and these complexes can serve as substrate for t4 polynucleotide ligase (ec 6.5.1.1). the rate of phosphodiester formation was found to be approximately the same as for the double-stranded complex and, furthermore, the rate appears to be similar on the two strands in the complex. joining of dt1o also took place in the presence of the double-stranded complexes dan.dtn and dan.run. polyamines increase the rate of joining catalyzed by t4 polynucleot ...	1978	687572
prognostic importance of the tumor-free laryngectomy specimen.	in a review of 500 consecutive laryngectomies, 36 specimens were found with no tumor. all patients had received radiation treatment preoperatively. patients with small primary tumors and no tumor evident in microscopically examined lymph nodes had excellent long-term survival. those patients with extensive (t4) primary tumors or with tumor found microscopically in their lymph nodes had poor survival statistics. this information reconfirms the known limitations of radiation and surgery in the cas ...	1978	687216
[serum thyroid hormones in hyperthyroid patients treated with carbimazole (author's transl)].	serum thyroxine (t4), triiodothyronine (t3) and thyrotropin (t.s.h.) were measured in 32 hyperthyroid patients treated with carbimazole. a discrepancy between serum t4 and t3 was present in 73 of 131 measurements: --normal t3 with increased t4 in 6 measurements; --increased t3 with normal t4 in 16 measurements: five times only there was a clinical hyperthyroidism; --normal t3 with decreased t4 in 49 measurements: three times only there was a clinical hypothyroidism. serum t.s.h. level was increa ...	1978	686661
[pathogenesis of endemic goiter: evidence of a dietary factor different from iodine deficiency (author's transl)].	the role of cassava was investigated in particularly severe endemic goiter area (ubangi). dietary intake of cassava induces an increase of plasma thiocyanate concentration and an increase of urinary excretion of stable iodine. generalized iodine deficiency induces very high thyroid uptakes; the other parameters of thyroid function are more or less affected according to the dietary intake of thiocyanate. when thiocyanate intake is high an adaptation is obtained only at the expense of a drop of pl ...	1978	686655
[amiodarone-induce "low t3-syndrome" (author's transl)].	amiodarone induces in patients "low t3-syndrome" characterized by normal t4. t3 resin uptake and free t3, contrasting with low t3 and free t4 values, and enhanced concentration of r-t3.	1978	686653
effects of thyroxine on protein turnover in rat skeletal muscle.	the effects of thyroxine (t4) on protein turnover in skeletal muscle were studied using normal, thyroidectomized (thyrex), and hypophysectomized (hypox) rats. thyrex rats had a depressed growth rate that was accompanied by 50% reductions in the level of rna and the rate of protein synthesis in gastrocnemius muscle, as determined in the perfused hemicorpus. protein synthetic efficiency (protein synthesis per unit rna) was decreased by 18%. daily treatment of thyrex rats with t4 at different dose ...	1978	686169
the effect of propranolol on serum levels of t4, t3 and reverse-t3 in hyperthyroidism.	the effect of propranolol (40 mg t.d.s.) on the peripheral levels of t4, t3 and reverse-t3 was studied in 26 patients with hyperthyroidism. the compounds were measured by specific radioimmunoassay techniques. the levels were followed for five weeks and compared with the levels before treatment. the results show that propranolol had no significant effect on the peripheral levels of t4 despite a rapid amelioration of clinical symptoms. a significant reduction of t3 levels was obtained during the f ...	1978	685727
[ring experiment for determining thyroid function: triidothyronine binding capacity (tbg), total thyroxine (t4), thyrotropin (htsh) and the effective thyroxine quotient (etr)].		1978	684188
mitigation of cretinism by breast-feeding.	an athyrotic infant had hypothyroidism at 1 year of age. he had grown at an above-average velocity until age 10 months when breast-feeding was discontinued, yet his bone age remained that of a newborn. these observations suggested that breast-feeding had attenuated hypothyroidism by providing significant quantities of thyroid hormones in the milk. to test this hypothesis, thyroxine (t4), 3,5,3'-triiodothyronine (t3), and 3,3,5'-triodothyronine (reverse t3) were measured in breast milk samples co ...	1978	683777
late endocrine effects of administering monosodium glutamate to neonatal rats.	rats were injected with monosodium 1-glutamate (msg) daily for the 1st 5 days of life and allowed to mature. this is known to cause selective destruction of neurons in the retina and in the arcuate nucleus of the hypothalamus. the adult animals had a significant increase in body fat without an increase in weight, a marked reduction in pituitary, thyroid, adrenal, gonadal and prostate weights. pituitary, hypothalamic and serum thyrotropin (tsh) were significantly reduced in the males. serum growt ...	1978	683472
inhibition of tsh-stimulated radioiodine turnover and release of t4 and t3 in vivo by somatostatin.		1978	682991
assembly of bacteriophage t4 head-related strucutres. ii. in vitro assembly of prehead-like structures.		1978	682197
the size of the bacteriophage t4 head in solution with comments about the dimension of virus particles as visualized by electron microscopy.		1978	682194
mutagenesis at a specific position in a dna sequence.	predefined changes in a known dna sequence were introduced by a general method. oligodeoxyribonucleotides complementary to positions 582 to 593 of the viral dna strand of the bacteriophage phix174 am3 mutant (pgtatcctacaaa), and to the wild type sequence in this region (pgtatcctacaaa), were synthesized and used as specific mutagens. each of these oligonucleotides was incorporated into a complete circular complementary strand when used as primer on a genetically heterologous viral strand template ...	1978	681366
patterns of primary thyroid failure.	serum thyroxine(t4), triiodothyronine (t3) and thyroid-stimulating hormone (tsh) levels were measured in 170 patients with suspected hypothyroidism. only patients with elevated serum tsh levels indicative of some degree of primary thyroid failure were included in the study. they appeared to represent four distinct classes of thyroid failure: class 1 (19.5%) had normal t4 and normal t3; class 2 (8%) had low t4 and elevated t3; class 3 (37%) had low t4 and normal t3; and class 4 (35.5%) had low t4 ...	1978	681161
thyroid function in acute intermittent porphyria: a neurogenic cause of hyperthyroidism?	thyroid function was investigated in 17 patients with acute intermittent porphyria (aip). in 13 patients in remission and latent for the disease, thyroid function was normal. in contrast, transient hyperthyoridism with elevated total t4, t3 free t4 and t3, and fti levels occurred in one pregnant patient with severe attacks of aip, and similar, though less pronounced, alterations in thyroid function were found in three other symptomatic female patients. it is suggested that increased thyroidal sy ...	1978	680630
non-glucoregulatory hormones (t4, t3, rt3, tsh, testosterone) during physical exercise in juvenile type diabetics.	non-glucoregulatory hormones (t4, t3, rt3, tsh and testosterone) were studied by radioimmunoassay in juvenile-type diabetics in moderate control and in ketosis due to insulin withdrawal and in age matched "normals" during a mild prolonged exercise test. the basal serum hormone levels revealed the following findings: serum testosterone was markedly lower in diabetics than in normals ( 177 +/- 24 resp. 618 +/- 52 ng/dl). this is in contrast to other studies, but it may reflect decreased testicular ...	1978	680627
hypothalamic-pituitary functions in patients with idiopathic pituitary dwarfism. further evidence for hypophysiotropic human deficiencies.	somatotropic, thyrotropic, gonadotropic and corticotropic functions in 10 patients with idiopathic hypopituitary dwarfism (ih) were investigated. the patients were divided into two groups: group i (5 patients) had normal plasma t4 levels, and group ii (5 patients) had t4 levels of less than 4.6 microgram/dl. in group i three cases had isolated growth hormone (gh) deficiency and two cases had gh and gonadotropin (gn) deficiencies; in group ii the 5 cases showed multiple anterior pituitary hormone ...	1978	679952
'low t3 syndrome' in patients chronically treated with an iodine-containing drug, amiodarone.	cardiac patients treated with the iodine-containing drug 'amiodarone' undergo a significant iodine overload which can last for months after the drug has been withdrawn. some patients develop hyperthyroidism and others hypothyroidism. in the hyper- or hypothyroid patients, the indices of thyroid function are modified as usually observed in these situations. in the patients remaining euthyroid while taking amiodarone or after its withdrawal, but still under its influence as shown by the iodine ove ...	1978	679500
the effects of thyroid hormones on myelination in the developing rat brain.	rats radiothyroidectomized 1 day after birth received daily subcutaneous injections of 1 microgram/10 g body weight of thyroxine (t4) or an equimolar amount of triiodothyroacetic acid (t3ac) from day 6 through day 25. the number of myelinated axons, myelinated axon area, and area of the myelin sheath in the corpus striatum were investigated. hypothyroid neonates demonstrated a normal number of myelinated axons with a decrease in the area of these axons. t4 treatment resulted in an increased numb ...	1978	678604
the role of irradiation in the management of head and neck cancer: analysis of results and causes of failure.	the records of patients with squamous cell carcinoma of the oral cavity, oropharynx, supraglottic and glottic larynx, whose primary lesion was treated with radiation therapy from 1964 through 1973, were reviewed. end points of the study are local control rate at the primary site, ultimate control with surgical salvage, and causes of failure. the local control rate at the primary site was 90% for t1 lesions, 80% for t2 lesions, and approximately 70% for selected t3 lesions. the control rates for ...	1978	675861
the synthesis of 25-hydroxycholecalciferol-23,23,24,24-t4 of high specific activity.	catalytic tritium reduction of cholest-5-en-23-yne-3beta,25-diol diacetate (viii) gave cholest-5-ene-3 beta,25-diol diacetate-23,23,24,24-t4 (ix) having a specific activity of 92 ci/mmol. bromination, dehydrobromination and hydrolysis of the labelled material gave cholesta-5,7-diene-3beta,25-diol-23,23,24,24-t4 (xi) which was photolyzed to the previtamin and then thermally equilibrated to 25-hydroxycholecalciferol-23,23,24,24-t4 (i).	1978	675737
[fundamental study on t4 peg riapac kit (author's transl)].		1978	674710
[blood level of thyroid hormones in surgical thyroid diseases. pre-,intra- and post-operative t3 and t4 levels].	a pre-, intra- and post-operative determination of thyroid hormone in values in the peripheral and thyroid vein reflux blood of patients with thyroid disorders requiring surgery showed that the so-called intraoperative squeezing of the gland either does not place or is of little importance when delicate surgical movements are commenced. a low incidence of intraoperative arrhythmia was also noted. prevention of the latter is not indicated, since it is often dependent on surgical movements and/or ...	1978	673215
[effect of estrogen upon thyroid metabolism (author's transl)].	during enhanced endogen estrogen production (pregnancy) as well as during exogen estrogen application a rise occurs in tbg concentration in serum, which seems to be dose related. simultaneously with the tbg there is an increase in total t4 and t3; the concentrations of the free t4 and t3 however decrease. towards the end of pregnancy aft4 is significantly decreased, compared to the controls, aft3 being in the lower normal range. the lower concentrations of the free hormones are also documented b ...	1978	672766
behavioral effects of neonatal thyroid hormones and differential postweaning rearing in rats.	male and female rats (t rats) given an injection of 75 microgram tri-iodothyronine (t3) and 25 microgram thyroxine (t4) on day 3 after birth were raised under enriched (e) and impoverished (i) postweaning conditions. observations of their open-field behavior and days 63 and 112 revealed a higher rate of activity in t groups as compared to controls (c) as well as faster running times and more entries/min in hebb-williams maze testing on days 74-86, indicating behavioral hyperactivity in t rats. a ...	1978	669060
transcription of bacteriophage t4 dna in vitro: selective initiation with dinucleotides.	the transcription products of phage t4 dna in vitro are separated on polyacrylamide gels. the influence of salt, polymerase, triphosphate concentration and glucosylation on the rna synthesis are shown. individual transcripts are initiated selectively with dinucleotides and a single triphosphate. this technique allows the prediction of the initiation sequences of several t4 transcripts.	1978	668702
decreased ratio of serum t3:rt3 in patients with hyperthyroidism.	in 14 hyperthyroid patient serum t4:rt3 ratio was significantly lower (399 +/- 20) than in the control subjects (572 +/- 20; p less than 0.001). a similar pattern was found for serum t3:rt3 ratio. in the hyperthyroid group the ratio was significantly lower (10.5 +/- 0.5) than in the control group (12.5 +/- 0.6; p less than 0.05). the data suggest that in hyperthyroidism the organism might shift conversion of t4 from biologically active t3 to poorly calorigenic rt3. it seems possible that the pro ...	1978	668639
effect of ethanol on the peripheral metabolism of thyroxine.	to determine the effect of ethanol on peripheral metabolism of t4, deiodinative clearance of t4 and serum concentration of t4, t3 and rt3 were measured in rats isotopically equilibrated with t4. ethanol 8 g/kg weight/day was given for 3 days to fasted rats and the results were compared with those normally fed or totally fasted animals. in fasted rats, deiodinative clearance of t4 and serum t4 and t3 concentration, and t3/t4 ratio, were decreased significantly, while serum rt3 concentration and s ...	1978	668634
thyroid function and polyamines. iii. changes in ornithine decarboxylase activity and polyamine contents in the rat thyroid during hyperplasia and involution.	changes in ornithine decarboxylase (odc) activity and in polyamine contents of the rat thyroid were studied under various experimental conditions. methylthiouracil (mtu) treatment produced several-fold increases in the thyroid odc activity and in the content of putrescine, spermidine and spermine within a week. while serum thyrotropin (tsh) levels increased gradually up to 3 weeks, the content of both putrescine and spermidine tended to reach a plateau after 2 weeks of the goitrogen treatment; s ...	1978	668626
[studies of thyroxine radioimmunoassay with a solid phase method--konsul t4 ria kit].		1978	668168
[experimental and clinical studies of seralute-t4 ria kit].		1978	668164
a radioimmunoassay for 3,5-diiodothyronine.	a radioimmunoassay for the measurement of 3,5-diiodothyronine (3,5-t2) using a 3h-labelled derivative of 3,5-t2 as tracer has been developed. the cross-reactivity of the antiserum was relatively high for t3 (3.1%) and low for t4 (less than 0.0015%). 3,5-t2 serum concentrations measured after fractional enrichment of 3,5-t2 were near or below the detection limit of 0.1 nmol/l in ten normal subjects and 0.38 nmol/l in five hyperthyroid patients. 3,5-t2 was also detected in thyroid tissue. the seru ...	1978	668156
synthesis of modified nucleoside 3',5'-bisphosphates and their incorporation into oligoribonucleotides with t4 rna ligase.	a simple procedure is described to prepare nucleoside 3'(2'),5'-bisphosphates from the corresponding nucleosides with the use of pyrophosphoryl chloride. this method is rapid, gives nearly quantitative yields and, most importantly, can be used for a variety of nucleosides with base and sugar modifications. since 3',5'-bisphosphates are donors in the t4 rna ligase reaction, a single residue can be enzymatically attached to the 3' end of oligoribonucleotides. by these procedures, five different ri ...	1978	667013
enzymatic oligoribonucleotide synthesis with t4 rna ligase.	the substrate specificity of t4 rna ligase has been examined to determine whether the intermolecular reaction is sufficiently general to realize its potential in the enzymatic synthesis of oligoribonucleotides of defined sequence. reactions between a variety of acceptor molecules with 3'- and 5'-bydroxyl groups and donor molecules with 3'- and 5'-phosphates indicate that the minimal substrates are a trinucleoside diphosphate acceptor and a nucleoside 3',5'-bisphosphate donor. increasing the chai ...	1978	667012
disturbed circadian variation of serum thyrotropin in patients with endogenous depression.	the relationship between the degree of depression and the circadian variation of serum tsh, t3 and t4 was investigated in 19 endogenously depressed patients. the difference between the hormone concentrations at 2 p.m. and at 12 p.m. was taken as an estimate of the magnitude of circadian variation. it was found that the circadian variation in serum tsh was inversely related to the degree of endogenous depression. this was mainly due to a diminution or absence of the night increase of tsh in sever ...	1978	665284
thyroid disease and function in breast cancer patients and non-hospitalized controls evaluated by determination of tsh, t3, rt3 and t4 levels in serum.	investigations concerning a possible influence of thyroid disease and function on the risk for breast cancer have given contradictory results--although a slight hypothyroidism was repeatedly suggested and supported by increased thyrotropin concentrations and exaggerated trh-responses. the selection of proper controls was stressed and in this study 179 consecutively diagnosed breast cancer patients were compared with 179 age-matched controls chosen from a computerized population register. the pre ...	1978	665106
[technical realization of the t3 and t4 tests as well as of the triiodothyronine radioimmunoassay].		1978	664812
antigenic gene products of bacteriophage t4 baseplates.		1978	664235
myxedema coma and normal serum thyroxine.	a 76-year-old man who developed the clinical syndrome of myxedema coma was found to have a normal serum thyroxine concentration. further investigation revealed the presence of a low serum t3 level before and after sodium levothyroxine therapy when the serum t4 value was in the hyperthyroid range. in addition, he demonstrated an inability to release tsh after intravenous administration of trh and was found to have an enlarged sella turcica. a partial defect in peripheral conversion of t4 to t3 is ...	1978	663737
[determination of serum thyroxine using ria-gnost t4 (author's transl)].		1978	663324
[fluorescent thyroid scanning and intrathyroidal iodine quantification: study in 140 patients (author's transl)].	the system consists of twenty individual sorces of one ci of 241am and a high resolution lithium drifted silicon detector. a dual channel analyser permits to measure intrathyroidal stable iodine. the scan requires only 13 minutes and can be made with a very low radiation dose (less than 10 mrads). it provides good quality images and remains available in cases of flooded iodine pools or iodine induced thyrotoxicosis. it is of great interest to follow up subacute thyroiditis and to diagnose autono ...	1978	662595
in vitro transcription and isolation of a polycistronic rna product of the t4 trna operon.		1978	661965
[neonatal screening for hypothyroidism. preliminary results from different methods (author's transl)].	in a regional study 2062 newborns were screened for congenital hypothyroidism by determination of tsh in plasma or dried blood spots on the fifth day of life. three newborns with congenital hypothyroidism were detected. all of them showed high tsh-levels between 462 and 2192 micromicron/ml. one newborn with a congenital goiter had a tsh-level of 143 micromicron/ml. additionally, in a follow-up study of 50 newborns with jaundice tsh, t4, t3 and reverse t3 during the first 120 h of life were measu ...	1978	661833
head maturation pathway of bacteriophages t4 and t2. iv. in vitro transformation of t4 head-related particles produced by mutants in gene 17 to capsid-like structures.	t4 mutants in gene 17 accumulate particles which contain the main head protein in the cleaved form (gp23*) arranged in an unexpanded lattice (empty small particles), together with other expanded capsids (empty large particles). the isolated empty small particles can be transformed in vitro, by lowering the ionic strength, to capsid-like structures. this structural transformaton is not coupled to chemical modification of the structural proteins of the empty small particles. in contrast to unexpan ...	1978	660719
uv-induced mutation in bacteriophage t4.	two late gene am mutants of bacteriophage t4 that can be induced to revert by uv were crossed to a temperature-sensitive ligase mutant. in the double mutants, uv-induced reversion was eliminated at a semirestrictive temperature. when the single am mutants were irradiated and then allowed a single passage in a permissive host, the uv-induced reversion frequency was increased by 15- to 25-fold. this increased mutagenesis was also abolished by the presence of the ligase allele. when the uv-irradiat ...	1978	660716
[serum t4 determination with thyroxine (t4) "eiken"--experimental and clinical studies].		1978	657558
investigations on the deiodination of thyroxine (t4) to 3,3'-diiodothyronine (3,3'-t2) in rat liver homogenate.	properties of an inactivating pathway of t4 to 3,3'-diiodothyronine (t2') in rat liver homogenate are described. the intermediate product (of this reaction sequence) is reverse t3 (rt3) which is very labile and cannot be measured at ph 7.5. the apparent km of the reaction rt3 to t2' is 2 x 10(-8) m. the activities catalyzing the reactions t4 to t3, t4 to t2' and rt3 to t2' are found in the 100 000 x g pellet. propylthiouracil inhibits all three activities to a similar degree; alpha-methyl-p-tyro ...	1978	657520
red cell 2,3-diphosphoglycerate and oxygen affinity of hemoglobin in patients with thyroid disorders.	we measured red blood cell 2,3-diphosphoglycerate (2,3-dpg), adenosine triphosphate (atp), and the p50 value in vitro of the oxyhemoglobin dissociation curve, which is the oxygen tension at half saturation of hemoglobin, in order to quantitate red blood cell oxygen transport function in individuals who were diagnosed as hypothyroid, euthyroid, or hyperthyroid based on measurements of thyroxine (t4), triiodothyronine (t3), thyrotropin (tsh), and their clinical status. hypothyroid (mean t4 2.8 mic ...	1978	656626
excision repair of ultraviolet damage in mammalian cells. evidence for two steps in the excision of pyrimidine dimers.	the incidence of pyrimidine dimer formation and the kinetics of dna repair in african green monkey kidney cv-1 cells after ultraviolet (uv) irradiation were studied by measuring survival, t4 endonuclease v-sensitive sites, the fraction of pyrimidine dimers in acid-insoluble dna as determined by thin layer chromatography (tlc), and repair replication. cv-1 cells exhibit a survival curve with extrapolation number n = 7.8 and do = 2.5 j/m2. pyrimidine dimers were lost from acid-insoluble dna more s ...	1978	656544
use of ethidium bromide fluorescence enhancement to detect duplex dna and dna bacteriophages during zone sedimentation in sucrose gradients: molecular weight of dna as a function of sedimentation rate.	duplex dna molecules and dna bacteriophages have been sedimented through 5--25% sucrose gradients containing ethidium bromide. the location of dna within the gradients has been determined by illuminating gradients with ultraviolet light and observing the ethidium bromide fluorescence enhancement induced by the dna. the relative sedimentation rates of linear, duplex dnas from bacteriophages t4, t5, t7 and an 8.3% t7 deletion mutant have been determined. the distances sedimented by dna have been c ...	1978	656381
effect of atp analogues on t4 polynucleotide ligase.		1978	656099
[the low serum triiodothyronine syndrome in nonthyroid diseases. distribution of plasma tsh concentrations].	t3 serum concentrations (ria) was low in 25 cases and normal low in 10 among 57 patients with serious systemic illnesses. these 35 patients were in clinical euthyroid state and had a normal t4 serum concentration and f.t.i. t.s.h. serum concentration was normal in 28 cases of 31 low t3 syndrome observed.	1978	655621
[serum t4, t3, rt3 and t.s.h. concentration in cancer (author's transl)].		1978	655619
[fetal and neonatal triiodothyronine (author's transl)].	thyroid hormones of amniotic fluids give informations about their presence in the fetus. t3 and t4, determined by radioimmunoassay, are found as soon as 12 weeks of gestation. their amounts are higher at 17 weeks than at 30-40 weeks of gestation. thyroid tissues of premature and full term neonates were analysed by chromatography after hydrolysis and 127i determined. t3 amount was very low near birth and increased with duration of survival. these results may be related to hormonal serum changes o ...	1978	655618
[in vitro synthesis of 3,5,3'-triiodothyronine catalyzed by thyroid peroxidase (author's transl)].	3,5,3'-triiodotyronine formation was studied in vitro after iodination of goiter thyroglobulin catalyzed by purified thyroid peroxidase. a fractionnal number of t3 is always obtained per mole of thyroglobulin in both in vitro and in vivo. this result cannot be explained by a heterogeneity in thyroglobulin iodination or in a partial conversion of t3 to t4. it is suggested that thyroglobulin is heterogenous either in its primary sequence or in its teritiary configuration. thyroglobulin contains ty ...	1978	655617
[interference of t4 in different serum t3 radioimmunologic determination kits].	the cross reactivity of t3 antibodies for t4 was studied with five t3 ria kits. t4 used was provided from t4 ria kits (abbot beckman, corning). the results are shown on the table indicating the concentration of t3 or t4 required to displace 50% of the t3 125 from anti t3. the cross reactivity for t4 of t3 antibodies from lepetit and phadebas was the same and was smaller than cross reactivity with the other laboratories. in clinical practice serum t4 levels of hypo and euthyroid subjects are not ...	1978	655615
[study of thyroxine synthesis in non-toxic goiters using dispersed thyroid cells (author's transl)].	the ability of thyroid tissue from non-toxic goiter to synthesis thyroid hormones was investigated by measuring 131 i-iodide incorporation in t4 in dispersed thyroid cell system. dispersed thyroid cells were prepared by trypsinization from 5 pathological and 2 normal thyroid specimens. the patients with non-toxic goiter were euthyroid or in mild hypothyroidism. the capacity of t4 synthesis was decreased in 4 cases and increased in 1 as compared to that of normal thyroid tissue. in one case, the ...	1978	655613
[results of therapy in cases of tumors of the nasal cavity and paranasal sinuses in relation to different tumor classifications (author's transl)].	the primary findings in 128 patients with malignancies of the nasal cavity and the paranasal sinuses were classified by 4 tumor classifications. the survival times and the rates of recurrences in relation to the stage of tumor were compared. mostly cancers of advanced stages t3 and t4 with bad prognosis were treated: only 35% of the examined patients survived longer than 5 years. there was no obvious difference in the results of therapy in tumor cases t3 and t4 in relation to the different tumor ...	1978	651499
binding of the structural protein soc to the head shell of bacteriophage t4.		1978	650689
neonatal thyrotoxicosis: alterations in serum concentrations of lats-protector, t4, t3, reverse t3, and 3,3't2.		1978	650321
the effect of thyroid hormones on rat pineal indoleamine metabolism in vitro.	the effect of tsh, triiodothyronine (t3) and thyroxine (t4) on basic and noradrenaline (na)-induced indoleamine metabolism was investigated in rat pineal culture based on the observation that, under the experimental system used, melatonin is formed from its amino acid precursor tryptophan. when added to the culture medium, alone or in the presence of na, tsh in dosages of 5 and 20 mu had no significant effect on pineal tryptophan metabolism.t4 and 2.5 microgram increased the melatonin concentrat ...	1978	650203
age-related glucose intolerance in hyperthyroid patients.	in an attempt to study age-related metabolic abnormalities, glucose intolerance and serum insulin were examined in normal subjects and hyperthyroid patients. for comparison, serum concentrations of thyroxine (t4), triiodothyronine (t3), and total cholesterol were also measured in normal subjects and hyperthyroid patients. although serum t4 concentration remained unchanged, serum t3 concentration decreased significantly in an elderly group of normal subjects. similarly, serum t4 did not change wi ...	1978	648744
propranolol withdrawal and thyroid hormones in patients with essential hypertension.	the effect of abrupt withdrawal of propranolol on serum concentrations of triiodothyronine (t3) and thyroxine (t4) was investigated in 5 patients with uncomplicated essential hypertension. the patients had been treated from 2 to 18 mo before the study was begun. doses varied from 160 to 480 mg propranolol daily. four of the patients studied developed tachycardia, sweating, or tremor within 2 to 6 days after withdrawal of propranolol. in 1 patient reversible ischemic ecg changes were recorded. th ...	1978	648076