Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [hyperthyroidism by autonomous metastasis of thyroid carcinoma (author's transl)]. | nine years after surgical ablation of a trabeculo-vesicular carcinoma of the tyroid, a patient developped bone and liver metastasis. she had clinical signs of thyrotoxicosis, clear increase of blood t3 and sligh increase of t4. the tsh secretion was blocked. exogenous tsh increased iodine uptake in the thyroid and not in the metastasis. after 2 doses of 120 mci of 131i, she became hypothyroid, the liver was normal and the scan revealed the disappearance of uptake in thyroid and in metastasis. su ... | 1976 | 1008508 |
| ability to perform prolonged physical exercise in dogs after chronic treatment with thyroxine. | treatment of dogs with l-thyroxine (t4) for 7 or 21 days had no influence on their body temperature, blood la and na concentrations and muscle glycogen content at rest. only blood ffa and glucose concentrations were significantly higher than those before t4 administration. during exercise performed by t4-treated dogs considerably higher increases in rectal temperature as well as in blood ffa and la concentrations were found in comparison with control runs. plasma na level at the end of exercise ... | 1976 | 1007928 |
| quantitative study of extrathyroid enzymatic conversion of t4 to t3 in man. | the authors present a quantitative study of the extrathyroid conversion process of t4 to t3 in 3 athyroid patients, on the basis of an original biochemical model and a rigorous mathematical treatment, utilizing the kinetic isotope method. the values of thor the constant of the reaction rate of conversion of t4 to t3 and of the t4 fraction that undergoes this process--serum concentrations of t4 and t2--were determined in two completely different ways: 1) the radiochromatographic method, and 2) ra ... | 1976 | 1006135 |
| normal conversion of thyroxine to triiodothyronine in a subject with iodotyrosine-dehalogenase deficiency. | bmr, t1/2 of serum t4, and the conversion of t4 to t3 were normal in a patient with iodotyrosine-dehalogenase deficiency who had undergone total surgical thyroidectomy and had been maintained on 200 mug of oral t4 daily. it is concluded that the enzyme system for monodeiodination and conversion of t4 to t3 is different than iodotyrosine-dehalogenase. | 1976 | 1004933 |
| [long-term critical evaluation of hypothyroidism after subtotal thyroidectomy for thyrotoxicosis]. | a retrospective study was made of 66 cases in which subtotal thyroidectomy had been carried for hyperthyroid syndromes, mainly of basedow type, with a view to establishing a more careful selection of thyrotoxicosis candidates for surgery or other management. the reasons for the different findings and post-operation evaluation of performance are analysed in the light of the time of follow-up, mostly within 5 to 15 yr of surgery. clinical examination in the light of statistical diagnostic indices ... | 1976 | 1004766 |
| stimulation of the synthesis of bacteriophage t4 gene 32 protein by ultraviolet light irradiation. | 1976 | 1003487 | |
| bacteriophage t4 prehead proteinase. ii. its cleavage from the product of gene 21 and regulation in phage-infected cells. | 1976 | 1003460 | |
| effects of propranolol on changes in heart rate, heart weight, kidney weight, urinary hydroxyproline and weight gain induced by large doses of thyroxine in the rat. | the effects of the beta-adrenergic receptor blocking agent, dl-propranolol, and of the antithyroid drug, carbimazole, upon some manifestations of thyroxine (t4)-induced changes in peripheral metabolism were studied in rats. propranolol lowered the heart rate, but did not alter the following changes induced by t4: increment in heart rate, increase in heart or kidney weight, increase in urinary hydroxyproline, decrease in body weight gain or increase in serum t4. carbimazole administration lowered ... | 1976 | 1003065 |
| marker effects on reversion of t4rii mutants. | the frequencies of 2-aminopurine- and 5-bromouracil-induced a:t leads to g:c transitions were compared at nonsense sites throughout the rii region of bacteriophage t4. these frequencies are influenced both by adjacent base pairs within the nonsense codons and by extracodonic factors. following 2ap treatment, they are high in amber (uag) and lower in opal (uga) codons than in allelic ochre (uaa) codons. in general, 5bu-induced transitions are more frequent in both amber and opal codons than in th ... | 1976 | 1001876 |
| effects of growth hormone, thyroxine, and age on diaphragm muscle from dwarf mice. | the ability of gh in vitro to stimulate leucine incorporation into protein and the uptake of 2-aminoisobutyric acid (aib) and 3-o-methyl-glucose (3-omg) was studied in diaphragm muscle from dwarf (dw/dw) mice. ovine gh (25 mug/ml) significantly enhanced the rate of protein synthesis in muscle from untreated dwarfs. in contrast, gh usually failed to stimulate transport, although occasionally a small increase in aib uptake was seen. insulin (50 muu/ml) readily stimulated both aib transport and pro ... | 1976 | 1001246 |
| [hybrids of phages lambda and t4]. | 1976 | 1001178 | |
| a comparison of the interference of fatty acids in the competitive binding radioassay and radioimmunoassay for serum t4. | 1976 | 1000836 | |
| thyroid function and metabolic state in chronic renal failure. | thirty-eight patients with chronic renal insufficiency who were in a dialysis program underwent studies of thyroid function and metabolic status. mean values for serum total and free thyroxine (t4) concentrations and thyroxine-binding globulin capacity were within normal limits. although mean serum total triiodothyronine (t3) concentration was normal, 43% of the group had low serum t3 and 54% had low serum free t3 concentrations. serum thyrotrophin (tsh) concentrations were normal in all but fou ... | 1976 | 999108 |
| control of cell growth. iii. direct mitogenic effect of thyroid hormones on an estrogen-dependent rat pituitary tumor cell line. | a possible direct estrogen requirement for growth of gh3/c14 rat pituitary tumor cells was evaluated in culture medium supplemented with estrogen-depleted serum prepared by a 56 degree c charcoal extraction procedure, and with serum obtained from ovariectomized sheep and ovariectomized adrenalectomized sheep. growth of the gh3/c14 cells in culture medium in which the final estrogen concentration was 2 pg/ml or less was equal to growth in medium with normal serum and equal to growth in the presen ... | 1976 | 994217 |
| an increase of plasma triiodothyronine concentration in man in a cold environment. | in an attempt to study an effect of cold on endocrine function in man, plasma thyroxine (t4), triiodothyronine (t3), tsh, and cortisol concentrations were studied in 24 healthy men who worked in a cold environment (4 to 6c) for 3 h for the culture of mushrooms and 56 healthy men who lived in cold environment in winter. for comparison, similar measurements were made on 47 university employees who lived in rooms with air-conditioning. in 24 adult men, acute exposure to cold for 3 h failed to affec ... | 1976 | 993317 |
| visual failure during replacement therapy in primary hypothyroidism with pituitary enlargement. | a 34-year-old woman with longstanding untreated thyroprivic hypothyroidism and pituitary enlargement is reported here in whom visual failure coincided with thyroid hormone replacement. visual fields were normal after 30 years untreated hypothyroidism, but severe concentric field constriction developed during the first 6 months of therapy and was relieved by hypophysectomy. plasma tsh and prolactin remained elevated during 10 months replacement therapy, but both were suppressed by preoperative hy ... | 1976 | 993314 |
| dna "melting" proteins. iv. fluorescence measurements of binding parameters for bacteriophage t4 gene 32-protein to mono-, oligo-, and polynucleotides. | the quenching of the intrinsic tryptophan fluorescence of t4-coded gene 32-protein on binding to nucleotide ligands, which was described in the preceding paper, is here exploited to measure thermodynamic parameters of the single-stranded nucleic acid-gene 32-protein interaction. it is shown that binding of small ligands follows a single site binding isotherm, with association constants increasing from approximately 20 m-1 for phosphate, to approximately 10(3) m for ribose or deoxyribose 5'-phosp ... | 1976 | 993212 |
| [suppression of dna-ligase deficiency and selective breeding of mutants and recombinants of t4 phage with phenotype rii]. | a number of methods which greatly simplify the introduction of frame shift mutations in the limited segment of the phage t4 rii genes were developed for studying genetical recombination between closely linked markers. these methods enable to construct multiple rii mutants with relatively small expenditure of labour. suppression of gene 30 deficiency by rii mutations has been studied for a variety of conditions. the results obtained indicate that riib polypeptide is involved in two different acti ... | 1976 | 992368 |
| determination of triiodothyronine in red blood cells by radioimmunoassay. | this study was undertaken to determine t3 content in red cells by radioimmunoassay. t3 in red blood cells was solubilized fairly from the stroma by hemolysis and red-cell t3 content could be determined directly by radioimmunoassay of the lysate. after hemolysing red cells with an equal volume of distilled water, 0.4 ml of the hemolyzate was used for the assay. the red-cell t3 content was expressed as ng/ml of red-cell volume. the normal t3 range in red cells was 0.20-0.45 ng/ml, and the mean+/-s ... | 1976 | 991832 |
| secretion of a bone resorbing factor by chick thyroid glands in organ culture. | the ability of the thyroid gland to secrete a bone resorbing factor in vitro was studied using glands obtained from 20-day-old chick embryos. the glands were incubated in a modified bgj medium containing 1 mg/ml bovine serum albumin under 5% co2-40% o2 at 37 c. the culture media were assayed in vitro by measuring the stimulation of the release of previously incorporated 45ca from cultured 19-day fetal rat bone shafts over a 48 h period. the glands secreted a stimulator of bone resorption which d ... | 1976 | 991827 |
| changes in serum thyroxine, triiodothyronine, and thyrotropin induced by lithium in normal subjects and in rats. | serum thyroxine (t4), triiodothyronine (t3), and thyrotropin (tsh) were measured in 4 normal subjects before, during, and 2 wk after administration of lithium carbonate (1,200 mg/day) for 6 wk. all subjects developed thyroid enlargement associated with increase in tsh. serum t3 and t4 did not change significantly. in 3 hypothyroid maintained on thyroxine replacement, lithium administration did not alter circulating levels of t4 or t3, suggesting no effect of lithium on the extrathyroidal metabol ... | 1976 | 991540 |
| an inter-laboratory comparison of total serum triiodothyronine determination. | l-triiodothyronine (t3) standards and an unknown serum were prepared and distributed to 18 different laboratories. t3 was determined on the standards and both t3 and t4 were assayed on the serum. the t3 and t4 results varied widely with a mean variance of 36 and 23%, respectively. there was, however, a positive correlation between the t3 values obtained within each laboratory and results of the unknown serum. thus, while the data of each laboratory is internally consistent, the data between labo ... | 1976 | 989996 |
| concentration of thyroid hormone in skeletal muscle. | 1. a modified method for the determination of thyroid hormone levels in muscle is described. 2. the levels of t4 and t3 in skeletal muscle tissue of rats lie in the same range. 3. the level of t4 in plasma is about a hundred times higher than that of t3. 4. to remove plasma hormones from the tissue in which hormone levels are to be determined, the animals should be perfused with saline before tissue samples are taken. | 1976 | 989664 |
| [serum t4 and t3 levels during administration of lugol's solution in basedow's disease]. | 1976 | 989471 | |
| an evaluation of lithium as an adjunct to carbimazole treatment in acute thyrotoxicosis. | the rate of control of thyrotoxicosis during the first 2 weeks of treatment was documented in 63 patients. twenty-three patients received carbimazole 40 mg plus lithium carbonate 750 mg daily and a comparable group of 20 patients were given carbimazole 40 mg plus potassium iodide 120 mg daily. in the lithium treated patients the mean percentage fall of serum t4 after 2 weeks treatment was 49% and the fall in serum t3 57%. the results were similar in the iodide treated patients; the mean falls in ... | 1976 | 989225 |
| t4 and t3 release from the perfused canine thyroid isolated in situ. | a method for once-through perfusion of the canine thyroid isolated in situ is described. the perfusion medium was a modified krebs ringer buffer with 4% dextran added. in 4 control experiments of the t4 and t3 concentratios in effluent were stable or slightly falling during 3 h perfusion. there were no significant alterations in the t4/t3 ratio in the effluent during these experiments. a 10-min infusion of bovine tsh (1 mu/ml) caused an increase in the release of t4 and t3 after 15-25 min. the t ... | 1976 | 989219 |
| sensitivity to lithium in treated graves' disease: effects on serum t4, t3 and reverse t3. | seven patients judged to be euthyroid following treatment of diffuse toxic goiter were studied to determine if they were susceptible to lithium induced hypothyroidism. lithium carbonate was administered for 4-7 weeks in a dosage (900 mg/day) which maintained serum lithium levels between 0.5-1.0 meq/l. blood was obtained weekly for the determination of serum 3,5,3'-triiodothyronine (t3), thyroxine (t4), 3,3',5'-triiodo-l-thyronine (reverse t3, rt3) and thyrotropin (tsh). values observed during li ... | 1976 | 989049 |
| [the relationship between the timing of the korotokoff sound (qkd) and the serum thyroid hormone concentrations]. | the interval between the onsets of the qrs complex and of the brachial korotokoff sounds at the diastolic pressure was termed qkd and it was already known that qkd was shortend in hyperthyroidism and prolongad in hypothyroidism distinctly. in the present study, simultaneous measurements of qkd, serum thyroxine (t4) and triiodothyronine (t3) concentrations, free t4 index (ft4i) and free t3 index (ft3i) were undertaken and attempts were made to examine the possible correlation of qkd to these para ... | 1976 | 987939 |
| [analysis of thyroid hormones in serum and urine by mass fragmentography using gc-ms]. | the first successful analysis of iodine compounds in serum and urine by mass fragmentography using gc-ms combined system was performed. the equipment used was shimadzu lkb 9000 gc-ms (mid-pm). the tmsi derivatives of the compounds were analyzed by gc-ms system equipped with a 3 ft x 3 mm column packed with 1% ov-1 and the temperature was programmed from 200 degrees to 320 degrees c at 10 degrees c/min. the mass specturm showed molecular ions at m/e 523, 649, 741, 867 and 993 which correspond to ... | 1976 | 987938 |
| [influencing of the thyroid hormone system by long-term treatment with 5-5 diphenylhydantion in children and adolescens (author's transl)]. | in the context of anticonvulsive maintenance therapy in epileptic children and adolescents, 5-5-diphenylhydantoin leads to a statistically significant decrease in total thyroxin (t4), protein-bound iodine (pbj) and free thyroxin (ft4). the consequences of dph therapy are based on the direct and indirect influence of dph on the thyroid hormone system. dph competitively pushes the t4 out of its plasma-protein bond. the lowering of t4, pbj and finally also ft4 can be traced back to an indirect infl ... | 1976 | 987507 |
| [reproducibility of emesis by orally administrated copper sulfate in cats]. | adult cats were administered oral threshold doses of copper sulfate every week. as the cats vomited in 70 out out 80 cases, the reproducibility was 88%. peripheral vomiting threshold dose was 40 mg/head or less, while the threshold dose for oral copper sulfate emesis after t4 transection and vagotomy was more than 160 mg/head. the following method is thus proposed for application in evaluating an antimetic for oral copper sulfate. adult cats are to be given the emetic once a week. the threshold ... | 1976 | 986987 |
| [tsh stimulation test using serum t3 and t4 as indicators]. | 1976 | 986885 | |
| diural tsh variations in hypothyroidism. | there is a circadian variation in serum tsh in euthyroid subjects. a similar diurnal variation has been demonstrated in patients with hypothyroidism. in the present study the 24-hour pattern of serum tsh was investigated in eight patients with hypothyroidism of varying severity and in five hypothyroid patients treated with thyroxine (t4). there was a circadian variation in serum tsh in patients with hypothyroidism of moderate degree, and in patients treated for severe hypothyrodism with thyroxin ... | 1976 | 985825 |
| recurrent goiter, hyperthyroidism, galactorrhea and amenorrhea due to a thyrotropin and prolactin-producing pituitary tumor. | a 22-year-old woman with recurrent goiter, hyperthyroidism, galactorrhea, and amenorrhea due to a pituitary tumor is described. she had been treated surgically twice for recurrent goiter with tracheal compression. despite clinical signs of hyperthyroidism and slightly elevated plasma thyroid hormone levels (t4: 11 mug/dl; t3: 189 ng/dl), without thyroid hormone replacement therapy the basal tsh level was elevated up to 23 muu/ml and could not be suppressed by exogenous thyroid hormones: even whe ... | 1976 | 985824 |
| morphologically recognizable markers for scanning immunoelectron microscopy. ii. an indirect method using t4 and tmv. | 1976 | 982828 | |
| radioimmunoassay for serum thyroxine. | a new procedure for radioimmunoassay (ria) was applied to determine the serum concentration of thyroxine (t4). mixtures of t4-methylester hydrochloride-bovine serum albumin complex and complete freund's adjuvant were injected to rabbits to get them immunized. specific anti-t4 rabbit serum, high in titer, could be obtained 18 months after the start of immunization. ria for t4 extracted by ethanol from serum was performed by an application of dextran-coated charcoal to separate bound and free 125- ... | 1976 | 982432 |
| freezing injury in bacteriophage t4. | 1976 | 981243 | |
| serum t3 and t4 levels in sick children. | 1976 | 980611 | |
| the dependence of hno2 mutagenesis in phage t4 on ligase and the lack of dependence of 2ap mutagenesis on repair functions. | a temperature sensitive ligase allele of phage t4 reduced or eliminated hno2 induced reversion of am mutants. since at the temperatures used, the ligase mutant is defective in the repair of some types of lethal lesions (i.e., uv, mms and ems induced lesions) these results indicate that hno2 mutagenesis may occur through a ligase dependent repair pathway. in contrast, 2ap induced mutation was not inhibited by mutants defective in the gene 30 ligase or in genes 32, 39, 41, 42, 44, 45, 46, 47, 49, ... | 1976 | 979960 |
| [successful treatment of thyroid storm by continuous plasmapheresis with a blood-cell separator (author's transl)]. | continuous plasmapheresis with the ibm blood cell separator showed to be a successful therapy in a 26 year old patient with thyroid storm. the patient regained consciousness already during plasmapheresis whereby 5 liters of plasma were exchanged within 3.5 h. although 633 mug thyroxine (t4) and 13.6 mug triiodothyronine (t3) were removed with the 51 plasma, no fall occurred in total serum t4 and t3 levels, probably due to the rapid reflux of thyroid hormones from the tissue into the intravascula ... | 1976 | 979079 |
| role of genes 46 and 47 in bacteriophage t4 reproduction. iii. formation of joint molecules in biparental recombination. | 1976 | 978724 | |
| studies of the structure of the t4 bacteriophage tail sheath. i. the recovery of three-dimensional structural information from the extended sheath. | 1976 | 978722 | |
| serum triiodothyronine determination in clinical use. | two radioimmunassays for the determination of serum triiodothyronine (t3) were developed. the assay of t3 in unextracted serum had several advantages over the assay on extracted serum and was chosen for the routine determination of t3 in serum from 117 patients requiring assessment of their thyroid status. in 53 subjects considered retrospectively not to have thyroid dysfunction nor to have been on steroid contraceptives or therapy, the pooled mean serum t3 concentration was 1-92 (actual range 0 ... | 1976 | 977774 |
| radioimmunoassay kits for thyroid hormones. | characteristics of the t4 and t3 radioimmunoassay kits that are commercially available in the uk are described. useful comparative data and criticism are given about many of these kits. some unacceptable variation in accuracy is demonstrated. | 1976 | 977766 |
| dose-response of prolactin and thyrotropin to n3im-methyl-thyrotropin releasing hormone in euthyroid men. | the synthetic n3im-methyl analogue of thyrotropin releasing hormone (methyl-trh) was administered intravenously to 15 euthyroid men, ages 36-62, in graded doses from 6.25 mug to 500 mug in order to establish the range of response of prolactin (prl), tsh, t3 and t4 to various doses of methyl-trh. there was a dose-related rise in serum tsh, prl, t3, and t4 which gave a nearly linear relationship when the integrated area of response was used as an index of response to the various doses of methyl-tr ... | 1976 | 977724 |
| a comparison of the effect of propylthiouracil on the respiration of thyroid slices from humans, chickens and rats. | 1. the qo2 of thyroid slices from hyperthyroid patients treated with propylthiouracil (ptu) was 140% that of slices from euthyroid patients. similar results were obtained using thyroid slices from ptu fed and control chickens. 2. ptu feeding caused thyroid hyperplasia, increased qo2 more than could be accounted for by the hyperplasia but neither increased qo2 of muscle, cerebral cortex or spleen nor altered serum t4 levels. 3. ptu in drinking water increased the size and vascularity of thyroids ... | 1976 | 976744 |
| compensatory thyroid hypertrophy after hemithyroidectomy in rats. | thyroid enlargement occurs in association with a variety of circumstances characterized by an impaired capacity of the gland to secrete adequate amounts of hormone. to elucidate the factors responsible for such compensatory thyroid growth, particularly the role of tsh, we have observed the response of the serum tsh, t3 and t4 concentrations following hemithyroidectomy in the rat, and have attempted to correlate changes in these functions with changes in the weight and histology of the thyroid re ... | 1976 | 976198 |
| pituitary thyrotropin (tsh) rebound phenomenon and kinetics of secretion in the goitrous rat: differential effects of thyroxine on synthesis and release of tsh. | the protein metabolism and [3h]-uridine uptake of thyroid and adenohypophysis and the kinetics of pituitary tsh rebound (ptr) were studied in goitrous female rats (fed propylthiouracil, ptu: for 7-12 weeks) following single, iv injections of l-thyroxine (t4: 0.8 to 200 mug). goitrogenesis was associated with reduced protein concentration and enhanced uptake of [3h] uridine in both glands. plasma levels of tsh were invariably elevated but stores in the adenohypophysis were consistently reduced. s ... | 1976 | 976195 |
| pharmacological influences on t4 to t3 conversion in rat liver. | five groups of 8 rats were treated with normal diet, diphenylhydantoin, dexamethasone, thyroxine (t4) and phenobarbital respectively for 14 days. after this period plasma triiodothyronine (t3), t4 and reverse t3 (rt3) concentrations as well as the t4 to t3 converting activity in the liver homogenates were determined. plasma t3 was low after dexamethasone, t4 and phenobarbital. plasma t4 was low after dexamethasone and elevated after t4. rt3 plasma concentrations were at the lower limit of detect ... | 1976 | 975586 |
| effect of fatty acids on thyroid function tests in vitro and in vivo. | addition of long-chain fatty acids to serum increased thyroxine (t4), measured by a competitive protein binding assay, and triiodothyronine (t3) uptake by sephadex or resin (t3u tests). this is compatible with the assumption that fatty acids compete with thyroxine for binding sites on t4-binding proteins. when equimolar concentrations of various saturated and unsaturated fatty acids were added to serum it was observed that the effectiveness in raising tests based on protein binding of thyroid ho ... | 1976 | 975583 |
| optimization of t assay: a model study. | factors involved in the optimization of competitive binding radioassays have been analyzed using a thyroxine (t4) radioassay as a model system. the effects of kinetic errors are minimized by the use of the lowest possible incubation temperature for the separation step and by the use of labeled t4 of high purity (which is not consistently available from commerical sources). the use of labeled t4 containing significant quantities of labeled triiodothyronine (t3) can lead to a marked increase in bi ... | 1976 | 975571 |
| the thyroidal response to chronic goitrogenic stimulation and the persistence of effects of early goitrogenic stimulation. | the effects of overstimulation of the thyroid gland induced by the feeding or rapeseed meal or thiouracil during the growing period persisted for as long as 75 weeks after withdrawal of the source of goitrogen from the diet. thyroid weight, thyroid iodine content and radioiodine uptake were all increased in birds which had received goitrogen during the growing period. follicle diameter was greater and the height of epithelial cells was lower than in control birds. the thyroid changes in these bi ... | 1976 | 974885 |
| replication of t4 dna in vitro. ii. assay system for and some properties of gene products required for t4 dna replication. | [3h]dttp was not incorporated into t4 dna in the in vitro system for t4 dna replication when the system was prepared from cells infected with t4 amber mutants defective in dna replication. [3h]dttp incorporation was resumed by adding the missing gene product to the defective system. dna replication by the reconstituted system proceeded by the discontinuous mode of replication, as observed in the wild-type system. by using this in vitro complementation system, molecular weights of gene 41, 43, 44 ... | 1976 | 972431 |
| structure of t4 polyheads. ii. a pathway of polyhead transformation as a model for t4 capsid maturation. | 1976 | 972397 | |
| goiter formation following prostaglandin administration in rats. | prostaglandins (pge1 and pge2) induced a hyperplastic microfollicular goiter with a high radioiodine (131i) thyroid uptake, increased endocytosis, a heavy autoradiographic (125i) reaction, and a moderate increase of thyroid hormones (t4, t3), thyroxine-binding globulin (tgb), and thyrotropin (tsh) concentrations in adult rats. ultrastructurally, both prostaglandins (e1 and e2) markedly stimulated the thyroid cell activity and increased the number of pseudopodia, the size of colloid and dense gra ... | 1976 | 970439 |
| effects of 3'-fluorothymidine, nalidixic acid, and mitomycin c on temperature-sensitive mutants of the dna polymerase of phage t4. | the temperature-sensitive dna polymerase mutator mutants a58 and l98 are less inhibited by 3'-fluorothymidine than the t4 wild type and the antimutator mutant cb121, in consequence of the assumed differences between the polymerase-associated exonuclease activities. this interpretation is confirmed by results with nalidixic acid and mitomycin c. the use of systems of different temperature-sensitive mutants of one or more genes is proposed for 1.) investigating the mode of action of drugs, 2.) st ... | 1976 | 969568 |
| subcellular membrane fatty acids of rat heart after cold acclimation or thyroxine. | relative percent changes in fatty acid (fa) composition of heart mitochondria and microsomes from 30-day-warm-acclimated and cold-acclimated rats were determined by gas chromatography. rats in both environmental groups received thyroxine (t4) (1 mg/kg per day) or propylthiouracil (ptu) (5 mg/rat per day) for 10 days prior to sacrifice. mitochondrial total unsaturated fatty acids (tufa) decreased in cold-acclimated rats relative to warm-acclimated controls but not in cold-acclimated animals recei ... | 1976 | 961919 |
| pathways of dna repair in t4 phage. ii. sedimentation analysis of intracellular dna in repair-defective mutants. | 1976 | 960557 | |
| [thyroid hormones and thyrotropin in liver and kidney insufficiency]. | in 22 patients with hepatic or renal insufficiency the serum concentrations of trijodothyronin, thyroxine and thyrotropin and also the t4-binding capacity of tbg were determined. the mean serum t3 concentration was found to be significantly lower in patients with hepatic coma when compared with euthyroid subjects. in the cases of renal insufficiency the serum t3 concentrations were in the normal range. due to hormone loss through dialysis however, the mean value of the t3 concentrations was slig ... | 1976 | 958900 |
| studies on obesity. iii. effect of triiodothyronine (t3) on thyroglobulin autoantibodies in euthyroid obese subjects. | effect of t3 therapy on tanned red cell agglutinating thyroglobulin (trc-tg) antibodies in 10 obese subjects without apparent thyroid disease was investigated. six other obese subjects without thyroid dysfunction and of approximately the same mean age who also had circulating trc-tg antibodies served as control subjects and were untreated. in vitro thyroid tests (tsh, total and free t4) performed before t3 therapy, as well as clinical examination, showed thyroid function to be normal in all subj ... | 1976 | 958004 |
| plasma catecholamine concentrations in hyperthyroidism and hypothyroidism. | using a modification of the fluorometric method of anton and sayre, we have measured the plasma epinephrine (e) and norepinephrine (ne) concentrations in patients with thyroid dysfunction. there was no significant difference in plasma e in hyperthyroid or hypothyroid subjects, the values being similar to those observed in normal subjects. there was a striking relationship between age and plasma ne in the euthyroid individuals (r = 0.685, p less than 0.001, n = 41). observed plasma ne concentrati ... | 1976 | 958003 |
| utilization of early promotors in mutant far p85 of bacteriophage t4. | we show that farp85 is a recessive mutant of t4 incapable of activating the delayed early promotors for genes 43 and 45 and that the farp85 mutation is in the same complementation group as the ts g1 mutation, which is located in the "modifier of transcription" (mot) gene. | 1976 | 957485 |
| mutator mutations in bacteriophage t4 gene 32 (dna unwinding protein). | bacteriophage t4 gene 32 encodes a dna unwinding protein required for dna replication, repair, and recombination. gene 32 temperature-sensitive mutations enhance virtually all base pair substitution mutation rates. | 1976 | 957480 |
| head length determination in bacteriophage t4: the role of the core protein p22. | 1976 | 957424 | |
| tsh measurements from blood spots on filter paper: a confirmatory screening test for neonatal hypothyroidism. | a sensitive radioimmunoassay for the measurement of tsh in the eluate of blood spotted on filter paper has been developed. the method is consistently sensitive to 0.1 to 0.25 muu of tsh and enables the detection of values equivalent to 6 to 15 muu/ml of serum. the measurement of tsh in the filter paper spot in all infants with low filter paper spot t4 has permitted rapid confirmation of 10 cases of neonatal hypothyroidism. however, cases of hypothalamic hypothyroidism with low or normal filter p ... | 1976 | 956996 |
| thyroid function in neonatal hypothyroidism. | various aspects of the thyroid function have been measured in 28 cases of neonatal hypothyroidism detected by means of the quebec screening program for metabolic diseases. in all instances the t4 value in the blood of filter paper spot was below 2 sd of the mean of the day, averaging 0.39 +/- 0.04 ng/40 mul (mean +/- sem) of eluted blood. the t4 value of a second similar sample averaged 0.22 +/- 0.04 ng/mul of eluted blood; this value was significantly lower than the first one. the serum t4 conc ... | 1976 | 956995 |
| thyroid-pituitary feedback during iodine repletion. | the changes in serum triiodothyronine (t3), thyroxine (t4), and thyrotropin (tsh) were measured during iodine repletion in a woman who was severely iodine-deficient because of a congenital iodide-trapping defect. serum t3 became detectable 12 h after iodine was begun and reached 68 ng/dl, a level within the normal range, by 36 h. it rose progressively during the first 9 days reaching a supra-normal level (200 ng/dl) and then fell slowly to 130 ng/dl. serum t4 was not detected (less than 1 mug/dl ... | 1976 | 956345 |
| plasma thyrotropin, thyroxine, and triiodothyronine relationships in man. | the physiologic relationships of plasma tsh, t4 and t3 levels measured every 20 min in seven healthy young men and one healthy young woman have been investigated. a nocturnal tsh surge was observed in all subjects on both nights of the 36-48 h baseline observation period. in males the maximum plasma tsh value occurred at 2300 h. the mean peak tsh level was 2.0 +/- 0.3 (se) muu/ml compared with a mean of 1.3 +/- 0.9 muu/ml for the entire baseline records of the 8 subjects. the effect of iv infusi ... | 1976 | 956341 |
| control of mutation frequency by bacteriophage t4 dna polymerase. ii. accuracy of nucleotide selection by the l88 mutator, cb120 antimutator, and wild type phage t4 dna polymerases. | the accuracy of nucleotide selection by wild type, l88 mutator, and cb120 antimutator t4 dna polymerases has been compared by measuring both stable incorporation of complementary and noncomplementary nucleotides into polymer and the dna-dependent conversion of deoxynucleoside triphosphate to monophosphate. the increased accuracy of the cb120 antimutator enzyme is shown by a ratio of utilization of incorrect to correct nucleotides with poly(da)-poly(dt) as template which is only 10 to 30% of that ... | 1976 | 956183 |
| control of mutation frequency by bacteriophage t4 dna polymerase. i. the cb120 antimutator dna polymerase is defective in strand displacement. | the ts cb1200 (antimutator) mutation in bacteriophage t4 dna polymerase increases the accuracy of dna replication since it results in a decrease in the frequency of mutations in other phage genes. the cb120 polymerases differs from the wild type enzyme in the slow rate at which it copies templates where primer extension requries displacement of polynucleotides base-paired to the template strand, even in the presence of the t4 dna unwinding protein (gene 32-protein). the ratio of nucleotides turn ... | 1976 | 956182 |
| bacterial characteristics determining the potential host range of bacteriophage t4. | many members of the enterobacteriaceae, but not other gram-negative organisms, apparently carry a specific recognition site for the t4 tail tube on their cytoplasmic membranes. | 1976 | 956114 |
| characterization of t4 mutants that partially suppress the inability of t4rii to grow in lambda lysogens. | in the course of isolating viable t4 deletions that affect plaque morphology (homyk and weil 1974), two closely linked point mutant, sip1 and sip2, were obtained. they map between genes t and 52, cause a reduction in plaque size and burst size, and partially suppress the lethality of rii mutants for growth in lambda lysogens. the characteristics demonstrate that sip1 and sip2 are similar to mutants previously reported by freedman and brenner(1972). in addition, d. hall (personal communication) h ... | 1976 | 955402 |
| the assessment of 125i treatment of thyrotoxicosis. | 257 patients have been reviewed 1-5 years (mean 3 years 2 months) after receiving one of five dose regimes of 125i for thyrotoxicosis. the cumulative incidence of hypothyroidism was 34% and of persistent thyrotoxicosis 17%. the group receiving doses between 351 and 500 muci/g had the highest proportion of euthyroid patients (65%) with the lowest requirement for repeat therapy (46%). in the euthyroid patients, increasing dose of 125i was associated with progressive decline in mean thyroxine (t4) ... | 1976 | 954217 |
| correlation between structural transformation and cleavage of the major head protein of t4 bacteriophage. | we have studied the maturation of t4 polyheads, the aberrant tubular structures related to the capsid of t4 bacteriophage. conditions have been found under which more than 95% of the major head protein (p23) undergoes the same cleavage that occurs during development of the normal capsid. the concomitant structural changes in the polyheads have been followed using electron microscope image filtering techniques. as a result of the cleavage, a radical transformation of the hexagonal lattice occurs, ... | 1976 | 954078 |
| triiodothyronine and thyroxine interrelationships in health and disease. | with the recent development of radioimmunoassay techniques for the measurement of serum triiodothyronine (t3) concentration, new concepts have arisen regarding the biologic role of t3 in health and disease and its interrelationships with thyroxine (t4). an awareness of the influence of clinical conditions that affect binding of thyroid hormone to plasma proteins is required in the interpretation of moderately increased or decreased serum t3 values. hormone preparations containing t3 may produce ... | 1976 | 953904 |
| a screening test for thyroid function. | the achilles tendon reflex half relaxation time measurement (art) has been used by many physicians both as a diagnostic test and for the assessment of progress in thyroid gland malfunction. reference is made to some results obtained in melbourne and in other countries using different methods of measurement of the art for these purposes. in a series of 2064 patients referred to the shepherd foundation centre, the achilles tendon reflex half relaxation time was measured by means of the smi reflexo ... | 1976 | 952638 |
| increased peroxidase activity in pendred's syndrome with hypothyroidism. | an 8-year-old boy with goiter and bilateral nerve deafness had a 46% discharge of radioiodine after thiocyanate administration. he was clinically euthyroid. although the serum total t4 was low (2.4 mug/100 ml) and tsh was significantly high (181 muu/ml), the serum total t3 was normal (152 ng/100ml). it was considered that the increased release of tsh by the feedback mechanism in response to the low t4 resulted in a quite normal level of serum t3. the thyroid gland demonstrated a low stable iodin ... | 1976 | 951708 |
| plasma thyroxine concentrations in ewes and their fetuses during the last six weeks of pregnancy. | changes in plasma thyroxine (t4) concentrations were followed in 27 fetal sheep after surgical implantation of catheters. fourteen days were required before stable concentrations of t4 were achieved, whether surgery was performed between 90 and 96 days or 109 and 120 days gestation. twenty-three fetuses were followed to birth, and during the last four days the t4 concentrations showed no change in 11 fetuses and a significant decrease in the other 12. birth occurred between 142 and 157 days gest ... | 1976 | 951516 |
| changes in serum triiodothyronine, thyroxine, and thyrotropin during treatment with thyroxine in severe primary hypothyroidism. | studies were performed on 40 patients with severe primary hypothyroidism, during treatment with varying doses of t4. therapy was initiated with 50 mug/day and was continued for at least 2 months. subsequent repeated increases of 25 mug/day were continued for at least 2 months until the serum tsh level was less than 10 muu/ml. measurements of serum t4 and t3, rt3u, and serum tsh were carried out at monthly intervals. trh tests were performed after tsh levels of less than 10 muu/ml had been attain ... | 1976 | 947930 |
| the specificity requirements of bacteriophage t4 lysozyme. | a series of bacterial cell wall glycopeptides of low molecular weight and cell wall nucleotide precursors have been tested for their inhibitory action on the digestion by t4 lysozyme of a radioactively labeled linear uncrosslinked peptidoglycan. the disaccharide-peptides glcnac-murnac-l-ala-d-glu(a2pm) (c5) and glcnac-murnac-l-ala-d-glu(a2pm-d-ala) (c6) as well as the monosaccharide-peptide murnac-l-ala-d-glu(a2pm) were found to be good competitive inhibitors (with similar ki values) whereas th ... | 1976 | 947753 |
| a simple and rapid thyroxine radioimmunoassy (t4-ria) in unextracted human serum; a comparison of t4-ria and t4 displacement assay, t4(d), in normal and pathologic sera. | a simple, rapid and accurate thyroxine radioimmunoassay (t4-ria) in unextracted serum or plasma has been described, and for comparison t4 determinations have also been made by a t4(d) procedure using abbott tetrasorb kits. t4-ria procedure basically involved denaturation of serum to dissociate t4-protein bond, and t4 released was allowed to react with [125i]t4-labeled t4 antiserum elicited by immunizing rabbits against bovine thyroglobulin. the displaced unbound [125i]t4 was rapidly taken up by ... | 1976 | 947623 |
| radioimmunoassay for 3,3',5'-triiodothyronine (reverse t3, r-t3) in unextracted human serum. | highly specific antibodies against 3,3',5'-triiodothyronine (reverse t3, r-t3) have been produced in rabbits. the crossreaction with t4 is about 0.05%. a radioimmunoassay for r-t3 in unextracted serum was developed. ans is used for blocking the binding of tracer and endogenous r-t3 to tbg. the sensitivity to the assay is 0.06 ng/ml plasma. the mean normal r-t3 concentration is 0.20 ng/ml. thyrotoxic patients show elevated levels; in most hypothyroid patients r-t3 concentrations are below the det ... | 1976 | 947601 |
| paraplegia following transurethral surgery. | we have presented a case of paraplegia following spinal anaesthesia administered for a transurethral prostatic resection. further investigation revealed a metastatic peridural compressive lesion at the level of t4. we have discussed some of the contributory factors involved, chiefly the lumbar puncture (perimedullary vascular engorgement, alterations in c.s.f. dynamics) and the possible role of intra-operative water intoxication (swelling of spinal cord cells). | 1976 | 947506 |
| the deiodination of thyroxine in hyperthyroid rats as determined by renal clearance of iodide. | other investigators have reported that whole body clearance of thyroxine (t4) is increased in hyperthyroid rats isotopically equilibrated with radioactive t4, using the 24 h post-injection serum t4 concentration in the clearance calculation. data from this laboratory indicate that serum t4 concentration is lowest at this point yielding falsely high clearance values, particularly when high doses of t4 are injected. to investigate this problem further, two types of experiments were performed. firs ... | 1976 | 947283 |
| reciprocal effects of thyroxine and triiodothyronine on their deiodination by rat tissues in vitro. | the reciprocal effects of loading doses of thyroxine (t4) or triiodothyronine (t3) on the deiodination of their 125iodine-labelled isotopes by rat muscle and liver homogenates were studied. in 21 experiments muscle homogenates deiodinated a mean 45.0% of a tracer dose of [125i]t4 and 18.0% of [125i]t3. on addition of graded amounts of nonradioactive t4 or t3 the percentual deiodination of both labelled hormones progressively declined. this effect was significantly greater in homogenates incubate ... | 1976 | 947124 |
| the effects of ergocornine on tail height, spontaneous and t4-induced metamorphosis and thyroidal uptake of radioiodide in neotenic ambystoma tigrinum. | 1976 | 946962 | |
| hyperthyroidism in graves disease. current trends in management and diagnosis. | the radioimmunoassay for t3 is now widely available and is a useful diagnostic tool for hyperthyroidism, especially in t3-thyrotoxicosis. it is an essential tool in the management of hyperthyroidism that persists after treatment with normal t4 serum levels or, in euthyroid cases, with low t4 serum levels. in these conditions, it reflects the metabolic state more accurately than serum levels of t4. a promising new test is the response of radioimmunoassayable tsh to protirelin (trh) administration ... | 1976 | 946941 |
| studies on the mechanism of action of potassium iodide on thyroid protein biosynthesis. | potassium iodide (ki) has been shown to have an antigoitrogenic action and to inhibit in vivo thyroid protein biosynthesis. beef thyroid slices were used to clarify further the mechanism of action of ki. incubations were performed in krebs-ringer-bicarbonate (krb) buffer under 95%o2 and 5% co2. ki caused a slight decrease in the uptake of [3h]eucine by the tissue. when labelled leucine incorporation into protein was measured it was found that 10(-6) m ki caused a marked inhibition. increasing co ... | 1976 | 946922 |
| iodothyronine content of human thyroid albumin. | stable thyroid hormones (t4 and t3)1) have been demonstrated in pure albumin isolated from normal human thyroid tissue iodinated in vivo. five samples of albumin were separated from other thyroid proteins by acrylamide gel electrophoresis. after pronase hydrolysis, the content of thyroid hormones was measured chemically (t4 + t3) as well as by competitive radioactive measurement (t4) and radioimmunoassay (t3). the purity of the albumin and validity of these measurements were confirmed by differe ... | 1976 | 946147 |
| ehanced biliary thyroxine excretion in rats treated with pregnenolone-16alpha-carbonitrile. | normal rats were treated with pregnenolone- 16alpha - carbonitrile (pcn) 10 mg/100 g by stomach tube twice daily for 3 days. in these animals the biliary excretion of intravenously injected 125i-thyroxine (t4) was enhanced and the bile: plasma 125i ratio (b/p ratio) and the biliary clearance rate of plasma 125i-t4 was increased. normal rats were treated with pcn for 3 days and homozygous gunn rats for 13 days. in both groups pcn enhanced the bile flow and elevated the b/p ratios and the biliary ... | 1976 | 946136 |
| transcriptional control of t4 coliphage-specific genes 30, 42, 43, riia, riib, and e. | escherichia coli b/r (suo) was infected, at 30 degrees c, with t4dam+, t4damb24-amn82 (i-, 44-, dna-negative phenotype), and t4damn134ambl292 (33-, 55-, maturation-defective phenotype). a genetic ('transformation') assay was used to monitor transcription of genes 30 (polynucleotide ligase), 42 (deoxycytidylate hydroxymethylase), 43 (dna polymerase), riia, riib, and e (endolysin). the principal results are: (i) all of the genes studied were transcribed exlusively from the so-called l-strand of ph ... | 1976 | 945327 |
| biochemistry of dna-defective mutants of bacteriophage t4. vi. biological functions of gene 42. | bacteriophage t4 gene 1 and 42 amber mutants (defective in deoxynucleoside monophosphate kinase and deoxycytidylate hydroxymethylase, respectively) are able to synthesize dna in cell-free lysates prepared as described by barry and alberts (1972), in contrast to their inabliity to do so in plasmolyzed and toluenized cell systems. addition of extracts containing an active gene 1 or 42 product has no effect on synthesis in lysates defective in the respective gene. thus, if these enzymes do play add ... | 1976 | 943575 |
| triiodothyronine radioimmunoassay and its application to thyroid disorders. | an accurate, highly sensitive triiodothyrone (t3) radioimmunoassay system is described. direct measurement in serum is made possible by the use of t3-free serum for standards, and blocking of t3 binding to thyroxine (t4)-binding globulin with salicylate. t4 cross reactivity was less than 0.3%. mean t3 levels in 55 euthyroid, 32 hyperthyroid and 19 primary hypothyroid patients were 1.46 +/- 0.17, 5.34 +/- 1.86 and 0.49 +/- 0.34 (sd) ng/ml, respectively. t3 was found to be a more sensitive index t ... | 1976 | 943381 |
| site of cleavage of superhelical phix174 replicative form dna by the single strand-specific neurospora crassa endonuclease. | experiments with the neurospora crassa single strand-specific endonuclease have provided evidence for the existence of regions of partially single-stranded character in covalently closed superhelical replicative form dna of phix174. the nuclease converts the superhelical molecules to either singly hit relaxed circular or doubly hit linear molecules. we show that the initial cleavage of phix174 superhelical dna is a "nick" bounded by a 5'-phosphate and a 3'-hydroxyl; no nucleotides are excised as ... | 1976 | 942717 |
| separation of labelled iodide, iodoprotein, iodotyrosines, and iodothronines in serum from patients treated with iodine-131, using an improved chromatographic method. | a method is described for complete separation of iodoprotein, 3-iodotyrosine (mit), 3,5-diiodotyrosine (dit), 3,5,3-triiodo-l-thyronine (t3), and l-throxine (t4) on a single column run on sephadex g-25 superfine in alkaline solution. sera from patients treated with 131i- have been analysed by this method after removal of i- by resin dialysis. | 1976 | 940981 |
| a mutation that confers temperature sensitivity on the translation of riib in bacteriophage t4. | 1976 | 940158 | |
| serum thyrotropin, prolactin, and growth hormone levels during the early neonatal period in the human infant. | normal term newborn infants have been studied during the first four hours after birth. cord blood and brachial venous blood samples were collected for measurement of serum prolactin (n = 15; 11 vaginal delivery; four cesarean section delivery), thyrotropin (n=8), growth hormone (n=7), tri-iodothyronine (n=7) and thyroxine (n=7). the mean cord serum tsh concentration was 12 +/- 2 muu/ml and peaked in all infants 30 minutes after birth (67 +/- 7 muu/ml). blood prl also increased in all of the 15 i ... | 1976 | 940027 |
| decreased pituitary growth hormone content in rats treated neonatally with high doses of l-thyroxine. | growth hormone and thyrotrophic hormone content have been measured by specific radioimmunoassays in anterior pituitaries of 22 day old rats. these animals were injected with saline or very high doses of l-thyroxine during the neonatal period in order to induce the "neo-t4" syndrome. growth of such animals is known to be affected. it was found that not only tsh but also gh content of the t4-treated animals was significantly lower than that of the saline-injected controls. | 1976 | 939463 |
| formation of 3,3'-diiodothyronine and 3',5',3-triiodothyronine (reverse t3) in thyroid glands of rats and in enzymatically iodinated thyroglobulin. | it was observed in the present investigation that labeled thyroxine (t4) comprised less than 2% of the total 131i in the thyroids of severely iodine-deficient rats labeled with 131i for 18-24 h, a much lower value than had previously been reported for iodine-deficient rats. this low value was attributable to two factors: 1) the use of a diet low enough in iodine content to produce extreme iodine deficiency, and 2) the use of a paper chromatography system that successfully separates t4 from the m ... | 1976 | 939197 |
| variations in the response of the thyroid gland of the rat to different low-iodine diets: correlation with iodine content of diet. | large variations are frequently encountered in the thyroidal responses of rats to commercially available low-iodine diets. the major aim of this investigation was to attempt to correlate these variations with differences in the iodine content of the diets. a method was developed for measuring the iodine content of low-iodine diets which was sufficiently sensitive to discriminate between a diet containing 15-20 ng of iodine per g and one containing 30-40 ng of iodine per g. large differences were ... | 1976 | 939196 |