Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| systematic replacement of amino acid residues within an arg-gly-asp-containing loop of foot-and-mouth disease virus and effect on cell recognition. | the conserved arg-gly-asp (rgd) motif found in a hypervariable, mobile antigenic loop of foot-and-mouth disease virus (fmdv) is critically involved in virus attachment to cells by binding to an integrin, probably related to alphavbeta3. here we describe (i) the synthesis of 241 15-mer peptides, which represent this loop of fmdv (isolate c-s8c1) and single variants in which each amino acid residue was replaced by 16 others and (ii) the inhibitory activity of these peptides on the ability of fmdv ... | 1996 | 8662712 |
| porcine polypyrimidine tract-binding protein stimulates translation initiation at the internal ribosome entry site of foot-and-mouth-disease virus. | the cdna for porcine polypyrimidine tract-binding protein (sptb) was cloned. the sptb amino acid sequence is highly homologous to the human ptb sequence (97% identity), and the sptb sequence corresponds to that of the longest human ptb, ptb4. the specificity of binding in the uv-crosslink of sptb to the internal ribosome entry site (ires) of foot-and-mouth-disease virus (fmdv) is similar to that of human ptb. purified recombinant sptb efficiently stimulates internal translation initiation direct ... | 1996 | 8654585 |
| chimeric hepatitis b virus core particles as probes for studying peptide-integrin interactions. | an rgd-containing epitope from the foot-and-mouth disease virus (fmdv) vp1 protein was inserted into the e1 loop of the hepatitis b virus core (hbc) protein. this chimeric protein was expressed at high levels in escherichia coli and spontaneously assembled into virus-like particles which could be readily purified. these fusion particles elicited high levels of both enzyme-linked immunosorbent assay- and fmdv-neutralizing antibodies in guinea pigs. the chimeric particles bound specifically to cul ... | 1996 | 8648742 |
| a 'mixed' self-assembled monolayer for an impedimetric immunosensor. | a synthetic peptide with the amino acid sequence 135-154 of the capsid protein vp1 of the foot-and-mouth-disease virus was modified with omega-hydroxyundecanethiol and applied together with non-derivatised omega-hydroxyundecanethiol for consecutive adsorption onto gold electrodes according to self-assembling procedures. the binding of a specific antibody to prepared recognition layers could be monitored by measurement of impedance or capacitance. in order to avoid non-specific effects, all measu ... | 1996 | 8639283 |
| the c-terminal domain of eukaryotic protein synthesis initiation factor (eif) 4g is sufficient to support cap-independent translation in the absence of eif4e. | the foot and mouth disease virus, a picornavirus, encodes two forms of a cysteine proteinase (leader or l protease) that bisects the eif4g polypeptide of the initiation factor complex eif4f into n-terminal (nt) and c-terminal (ct) domains. previously we showed that, although in vitro cleavage of the translation initiation factor, eif4g, with l protease decreases cap-dependent translation, the cleavage products themselves may directly promote cap-dependent protein synthesis. we now demonstrate th ... | 1996 | 8635470 |
| rapid and sensitive polymerase chain reaction based detection and typing of foot-and-mouth disease virus in clinical samples and cell culture isolates, combined with a simultaneous differentiation with other genomically and/or symptomatically related viruses. | reverse transcription followed by the polymerase chain reaction method (pcr) allowed the detection of foot-and-mouth disease virus (fmdv), regardless of the serotype. a primer set corresponding to highly conserved regions of the 2b sequence was selected. by combining in a single reaction tube specific primer pairs for fmdv, swine vesicular disease virus, (svdv), encephalomyocarditis virus (emcv) and bovine viral diarrhea virus (bvdv), all four viruses could be identified and differentiated in on ... | 1996 | 8634024 |
| detection of foot-and-mouth disease viral sequences in various fluids and tissues during persistence of the virus in cattle. | to assess whether foot-and-mouth disease virus (fmdv)-specific sequences could be identified in tissues from persistently virus-infected animals. | 1996 | 8633795 |
| recognition of foot-and-mouth disease virus and its capsid protein vp1 by bovine peripheral t lymphocytes. | the role of t cells in immunity to foot-and-mouth disease virus is still poorly defined compared to that of the humoral response. in this paper we describe a systematic, longitudinal study on the cellular recognition of fmdv and its subunit protein vp1 by bovine peripheral blood t lymphocytes. multiple vaccination with a single virus serotype induced a serotype cross-reactive proliferative t cell repertoire that varied in magnitude between individual animals and with the serotype of the vaccine ... | 1996 | 8627261 |
| recognition of the initiation codon for protein synthesis in foot-and-mouth disease virus rna. | foot-and-mouth disease virus (fmdv) rna utilizes two in-frame initiation codons to produce two precursor proteins with identical carboxy termini. the 5' untranslated region (5'utr) directs the ribosome to internal sequences without the need for a cap structure as used in host mrnas. the fmdv 5'utr was cloned upstream of the reporter gene chloramphenicol acetyltransferase (cat) in order to study the selection of initiation site and to facilitate quantification of the translation products. after i ... | 1996 | 8627230 |
| antibody and host cell recognition of foot-and-mouth disease virus (serotype c) cleaved at the arg-gly-asp (rgd) motif: a structural interpretation. | foot-and-mouth disease virus (fmdv) of serotype c (isolate c-s8c1) was cleaved in situ by trypsin at the arg-gly-asp (rgd) motif, which is involved both in attachment of fmdv to cells and in recognition of a major antigenic site (site a) by antibodies. though 99.4% of the rgd moieties were cleaved, the virus remained infectious. a synthetic peptide which represented the sequence of the vp1 g-h loop of c-s8c1, including the rgd motif, greatly inhibited fmdv attachment to cells. the same peptide i ... | 1996 | 8627229 |
| a protective anti-peptide antibody against the immunodominant site of the a24 cruzeiro strain of foot-and-mouth disease virus and its reactivity with other subtype viruses containing the same minimum binding sequence. | a synthetic peptide vaccine of the general sequence cys-cys-(200-213)-pro-pro-ser-(l41-158)-pro-cys-gly(peptide a40), where the numbered residues refer to the vp1 sequence of foot-and-mouth disease virus (fmdv) strain a24 cruzeiro, has previously been shown to elicit neutralizing and protective antibodies in guinea-pigs and cattle. to examine this immunogenic tract in more detail monoclonal antibodies (mabs) were raised to this peptide. one such mab c1.1, which recognized the homologous peptide, ... | 1996 | 8609466 |
| genetic variation of the poliovirus genome with two vpg coding units. | amongst the picornaviruses, poliovirus encodes a single copy of the genome-linked protein, vpg wheras foot-and-mouth disease virus uniquely encodes three copies of vpg. we have previously shown that a genetically engineered poliovirus genome containing two tandemly arranged vpgs is quasi-infectious (qi) that, upon genome replication, inadvertently deleted one complete vpg sequence. using two genetically marked viral genomes with two vpg sequences, we now provide evidence that this deletion occur ... | 1996 | 8598203 |
| susceptibility of llamas (lama glama) to infection with foot-and-mouth-disease virus. | an experimental trial was conducted to evaluate the ability of foot-and-mouth-disease (fmd) virus (serotypes a79, c3, o1) to infect susceptible llamas exposed either directly to affected livestock, or indirectly to llamas that had been directly exposed to affected livestock. in addition, susceptible livestock species (cattle, pigs, goats, and sheep) were exposed to those llamas that had been both directly and indirectly exposed to the fmd virus to further look at potential transmission possibili ... | 1995 | 8594845 |
| induced pocket to accommodate the cell attachment arg-gly-asp motif in a neutralizing antibody against foot-and-mouth-disease virus. | the three-dimensional structure of the fab fragment of a neutralizing monoclonal antibody (sd6) elicited against foot-and-mouth disease virus (fmdv) has been determined at 2.5 a resolution and refined to a crystallography agreement r-factor of 0.186. the structure has been compared with that of the same fab molecule complexes with a 15 amino acid peptide (a15) representing a major antigenic site of fmdv, and determined at 2.8 a resolution. the fab quaternary structure, defined both by the elbow ... | 1996 | 8594203 |
| serological comparison of type a foot and mouth disease virus isolates from thailand. | antigenic variation of type a foot and mouth disease (fmd) virus in thailand was examined using a total of 82 field viruses isolated between 1986 and 1989. a two-dimensional serum microneutralisation test was used to compare these isolates to a reference strain, a15 bangkok 1960 (a bkk/60). viruses regarded as unrelated to a bkk/60 were compared to another reference strain, a22 nakhon pathom 1986 (a npt/86). this approach divided the viruses into two groups. most of the viruses shared a close an ... | 1995 | 8593389 |
| serological comparison of type asia 1 foot and mouth disease virus isolates from thailand. | antigenic variation of type asia 1 foot and mouth disease (fmd) virus in thailand was examined using a total of 50 field viruses isolated between 1986 and 1992. a two-dimensional serum neutralisation test was used to calculate r values for comparison of these isolates with a reference vaccine strain, asia 1 bangkok 1960 (bkk/60). viruses were also compared to two field isolates, asia 1 36-2/88 and asia 1 45/88, and some were compared to another vaccine strain, asia 1 nakhon pathom 1984 (npt/84). ... | 1995 | 8593388 |
| selection of vaccine strains of foot and mouth disease virus for use in southern africa. | in the countries of southern africa, types sat 1, sat 2 and sat 3 (sat: southern african territories) of foot and mouth disease (fmd) virus are the most widely represented, especially the sat 2 virus. since 1982, examinations have been conducted on 139 isolates of these virus types. other viruses, types o and a, have been detected in the north of this area. the typing and sub-typing of viruses with the complement fixation (cf) test can be improved by using panels of monoclonal antibodies (mabs), ... | 1995 | 8593387 |
| structural comparison of two strains of foot-and-mouth disease virus subtype o1 and a laboratory antigenic variant, g67. | foot-and-mouth disease viruses (fmdvs) are members of the picornavirus family and cause an economically important disease of cloven-hoofed animals. to understand the structural basis of antigenic variation in fmdv, we have determined the structures of two viruses closely related to strain o1bfs whose structure is known. | 1995 | 8590018 |
| assessment of foot and mouth disease vaccine potency by liquid-phase blocking elisa: a proposal for an alternative to the challenge procedure in argentina. | the lowest expected protection (lep) at a 95% confidence of 245 foot and mouth disease (fmd) commercial vaccines was calculated from the titres of liquid-phase blocking sandwich elisa (lpelisa) of cattle sera obtained from 3920 animals at 60 days post-vaccination (d.p.v.) and challenged with live virus at 90 d.p.v. it was found that lep evaluation is highly specific (i.e. it is able to predict the failure in 100% of the cases) although its ability to predict the challenge (pg test) approval (i.e ... | 1995 | 8585292 |
| [diagnosis of contagious diseases in animals using pcr]. | the pcr is used for diagnostic purposes as it allows to detect infections agents within a much shorter time than by cultural isolation. in addition, it can detect non-infectious viruses and bacteria in clinical samples. these advantages are important factors in the diagnosis of highly contagious animal diseases (mainly caused by viruses) since a rapid laboratory diagnosis will allow to take immediate disease control actions. pcr is routinely used at the institute of african and classical swine f ... | 1995 | 8584867 |
| the persistence of foot-and-mouth disease virus on wool. | five suffolk sheep, held in a high-security isolation room, were exposed for 2 hours to the aerosol of 3 mature pigs that had been infected with foot-and-mouth disease virus (fmdv), strain o1-bfs. the fleeces of 3 of the sheep were contaminated with fmdv at 2 days post exposure (dpe), while at 5 dpe the fleeces of all 5 sheep were more extensively, and more heavily, contaminated. the persistence of fmdv on contaminated wool was examined in vitro using multiple 0.5 g samples of merino wool that w ... | 1995 | 8579558 |
| structure and immunogenicity of experimental foot-and-mouth disease and poliomyelitis vaccines. | the physico-chemical properties and immunogenicity of experimental vaccines against foot-and-mouth disease (fmd) and poliomyelitis, prepared by treatment of the viruses with n-acetylethyleneimine (aei), formaldehyde or neutral red, have been studied. none of these reagents affects the rate of sedimentation of the particles or their reaction with antibody against the major immunogenic sites. fmd vaccines prepared by inactivation with aei or neutral red, behaved like the untreated virus, in that t ... | 1995 | 8578849 |
| equine rhinovirus 1 is more closely related to foot-and-mouth disease virus than to other picornaviruses. | equine rhinovirus 1 (erhv1) is a respiratory pathogen of horses which has an uncertain taxonomic status. we have determined the nucleotide sequence of the erhv1 genome except for a small region at the 5' end. the predicted polyprotein was encoded by 6741 nucleotides and possessed a typical picornavirus proteolytic cleavage pattern, including a leader polypeptide. the genomic structure and predicted amino acid sequence of erhv1 were more similar to those of foot-and-mouth disease viruses (fmdvs), ... | 1996 | 8577774 |
| a highly divergent antigenic site of foot-and-mouth disease virus retains its immunodominance. | the ability of a highly divergent antigenic site of foot-and-mouth disease virus (fmdv) of serotype c to elicit neutralizing antibodies has been evaluated in mice and rabbits. the viruses compared, fmdv c-s8c1 and hr, differ in a single amino acid replacement in their capsid proteins, but represent two extreme antigenic specificities of the major antigenic site a of fmdv type c. both, studies of cross-neutralization of homologous and heterologous virus, and fractionation of site a-specific antib ... | 1995 | 8546800 |
| sequence identification of antigenic variants in plaque isolates of foot-and-mouth disease virus. | foot-and-mouth disease virus isolates frequently contain mixtures of antigenic variants. using synthetic mixtures of two 'pure' viruses which differed at one amino acid of the major epitope, it was found that a minor component present as 10% or less of the mixture would be undetected by nucleic acid sequencing. | 1995 | 8537465 |
| localization of foot and mouth disease virus rna in tissue culture infected cells via in situ polymerase chain reaction. | foot and mouth disease virus rna was visualized in infected primary tissue culture cells by in situ pcr incorporating digoxigenin-labeled dutp. the viral rna polymerase gene was used as a target for amplification. infected cells revealed cytoplasmic staining, predominantly perinuclear. the intensity of staining was in proportion to the degree of cytopathology observed and similar to the results obtained using immunoperoxidase staining. the in situ pcr technique for fmdv detection could be applie ... | 1995 | 8530568 |
| identification of native foot-and-mouth disease virus non-structural protein 2c as a serological indicator to differentiate infected from vaccinated livestock. | cattle and pigs which have been vaccinated against foot-and-mouth disease can be distinguished from convalescent animals by radio-immunoprecipitation and sodium dodecyl sulphate polyacrylamide gel electrophoresis of the virus-induced proteins reacting with the respective sera. baby hamster kidney cells infected with foot-and-mouth disease virus (fmdv) (serotype a24) were labelled with 35s-methionine and the virus-induced proteins were precipitated with sera from vaccinated and subsequently chall ... | 1995 | 8525090 |
| dilute passage promotes expression of genetic and phenotypic variants of human immunodeficiency virus type 1 in cell culture. | we have studied the extent of genetic and phenotypic diversification of human immunodeficiency virus type 1 (hiv-1) upon 15 serial passages of clonal viral populations in mt-4 cell cultures. several genetic and phenotypic modifications previously noted during evolution of hiv-1 in infected humans were also observed upon passages of the virus in cell culture. notably, the transition from non-syncytium-inducing to syncytium-inducing phenotype (previously observed during disease progression) and fi ... | 1993 | 8474182 |
| molecular epidemiology of foot-and-mouth disease virus type o. | a phylogenetic tree based on the vpi sequences of type o foot-and-mouth disease virus (fmdv) has been derived. direct sequencing of pcr products has been used to obtain the vp1 gene sequences of new isolates. the tree exhibits four main lineages that largely correlate with the geographical origin of isolates. the analysis supports a close relationship between european o1 field isolates and vaccine strains, with the exception of o thalheim aus/81 and o wuppertal ger/82 which were probably of non- ... | 1993 | 8409952 |
| recent advances in bovine vaccine technology. | a description of new commercial and experimental vaccines for viral and bacterial diseases of cattle can be broadly divided into those used for both beef and dairy cows and those used predominantly in dairy cattle. for both types of cattle, newer and experimental vaccines are directed against several of the important viral (e.g., bovine herpesvirus 1, bovine viral diarrhea virus, bovine respiratory syncytial virus, parainfluenza type 3, and foot-and-mouth disease virus) and bacterial pathogens ( ... | 1993 | 8408872 |
| cross-reactive idiotopes among anti-foot and mouth disease virus neutralizing antibodies. | foot and mouth disease virus (fmdv) viral protein 1 is the only one of the four viral proteins (vp) that induces neutralizing antibodies as an isolated protein. a 32 amino acid (aa) residue (32dimer) of fmdv subtype a12 lp ab vp1 (aa 137-168) was immunogenic against the a12 subtype. three antibody populations each recognizing different epitopes on 32dimer were isolated by affinity chromatography (afc) from the serum of a steer which had been immunized with the 32dimer. the 32dimer contains an aa ... | 1993 | 8406565 |
| distinctive features of foot-and-mouth disease virus, a member of the picornavirus family; aspects of virus protein synthesis, protein processing and structure. | 1993 | 8396787 | |
| network models for sequence evolution. | we introduce a general class of models for sequence evolution that includes network phylogenies. networks, a generalization of strictly tree-like phylogenies, are proposed to model situations where multiple lineages contribute to the observed sequences. an algorithm to compute the probability distribution of binary character-state configurations is presented and statistical inference for this model is developed in a likelihood framework. a stepwise procedure based on likelihood ratios is used to ... | 1993 | 8395605 |
| the detection of foot-and-mouth disease virus in oesophageal-pharyngeal samples by a polymerase chain reaction technique. | a polymerase chain reaction (pcr) technique was used to detect the presence of foot-and-mouth disease virus (fmdv) in oesophageal-pharyngeal(op) samples from experimentally infected steers. ten-fold dilutions of op samples were also diluted, inoculated onto lamb kidney cell cultures, and incubated overnight. the cultures that did not show overt cytopathogenic effects (cpe) of fmdv infection were frozen and thawed; both the fluid and the cell pellet were tested by the pcr technique. the pcr detec ... | 1993 | 8395537 |
| protection of swine against foot-and-mouth disease with viral capsid proteins expressed in heterologous systems. | three groups of swine were each inoculated with a different antigen preparation of foot-and-mouth disease virus (fmdv) capsid proteins and challenged by contact exposure to animals infected with fmdv. one group of four animals was inoculated with an extract from cells infected with a recombinant baculovirus containing the fmdv p1-2a structural protein precursor gene and a portion of the p2 gene. two out of four animals were protected from clinical disease, but not from virus replication. a secon ... | 1993 | 8395128 |
| genetically engineered foot-and-mouth disease viruses with poly(c) tracts of two nucleotides are virulent in mice. | to determine the role of the poly(c) tract found at the 5' end of the genome of foot-and-mouth disease virus, synthetic rnas (in vitro transcripts) with poly(c) tracts of different lengths have been produced and evaluated. rnas with poly(c) tracts of 35, 25, 16, 6, or 2 residues displayed similar specific infectivities in baby hamster kidney (bhk) cells. viruses recovered from cells transfected with in vitro transcripts containing 6 to 35 cs had properties similar to those of the wild-type virus ... | 1993 | 8394441 |
| identification of a fifth neutralizable site on type o foot-and-mouth disease virus following characterization of single and quintuple monoclonal antibody escape mutants. | a monoclonal antibody (c3) produced against foot-and-mouth disease virus type o1caseros was found to neutralize quadrivalent monoclonal antibody escape mutant (g67) of foot-and-mouth disease virus type o1kaufbeuren. this mutant had been characterized at the sequence level as having distinct changes affecting four non-overlapping neutralizable sites. the c3 monoclonal antibody was used to prepare a quintuple escape mutant from the g67 and a single escape mutant from the parental o1kaufbeuren viru ... | 1993 | 8393912 |
| a comparative study of the immune responses of sheep against foot-and-mouth disease virus types asia-1 and o peg-concentrated aluminium hydroxide gel and oil-adjuvanted vaccines. | foot-and-mouth disease (fmd) vaccines prepared against types asia-1 and o peg-concentrated and adjuvanted with aluminium hydroxide gel were compared with oil-adjuvanted types asia-1 and o vaccines in sheep. the study was conducted by inoculating 0.5 ml of monovalent vaccine under laboratory conditions and 1 ml dose of bivalent vaccine in field conditions. the antibody responses were monitored by serum neutralization and elisa tests. the results indicate that peg-concentrated gel vaccine was of c ... | 1993 | 8393608 |
| large-scale use of liquid-phase blocking sandwich elisa for the evaluation of protective immunity against aphthovirus in cattle vaccinated with oil-adjuvanted vaccines in argentina. | specific serum activity levels against four reference strains of foot-and-mouth disease virus (fmdv) were evaluated from 1634 animals vaccinated with commercial quadrivalent oil vaccines and from 746 unvaccinated, naive animals, using the liquid-phase blocking sandwich elisa (lpelisa) test. cows from the fmdv-free area of argentina were tested for the absence of specific fmdv antibodies (sp fmdv abs) and those showing lpelisa titres < 1.0 were grouped in lots of 16 animals. they were vaccinated ... | 1993 | 8393607 |
| the carrier state in foot and mouth disease--an immunological review. | the carrier state in foot and mouth disease (fmd) is characterized by the asymptomatic low-level excretion of foot and mouth disease virus (fmdv) from the oropharynx of ruminants for periods that are species and virus strain-dependent. persistent infection with fmdv readily occurs following the failure of virus elimination at the acute stage of infection, a process thought to be mediated through the phagocytosis of antibody/virus immune complexes. recent evidence supports the view that carrier c ... | 1993 | 8392891 |
| rhipicephalus zambeziensis unlikely to transmit foot-and-mouth disease virus. | the potential of the ixodid tick, rhipicephalus zambeziensis, was investigated as a vector in the transstadial transmission of the foot-and-mouth disease virus by feeding nymphae on viraemic (log 1.0-4.0 tcid50/ml) cattle. suspensions were prepared, at various intervals after detachment, from pools of engorged nymphae--some of which were allowed to moult first. suspensions were inoculated into sucking mice, cell cultures and, in some cases, cattle to detect the fmd virus. newly moulted adult tic ... | 1993 | 8392681 |
| detection of foot-and-mouth disease virus rna in clinical samples and cell culture isolates by amplification of the capsid coding region. | foot-and-mouth disease is one of the most economically important virus diseases of livestock. two important requirements for the control of this disease are rapid laboratory diagnosis and epidemiological investigation. the use of the polymerase chain reaction method (pcr) to amplify specific nucleic acid regions offers the unique possibility of combining swift viral detection with the production of genetic material suitable for sequencing and other methods of molecular epidemiological analysis. ... | 1993 | 8391540 |
| a single nucleotide substitution in the internal ribosome entry site of foot-and-mouth disease virus leads to enhanced cap-independent translation in vivo. | mutants of foot-and-mouth disease virus (fmdv) with altered biological properties can be selected during the course of persistent infection of bhk-21 cells with fmdv c-s8c1 (j. c. de la torre, e. martínez-salas, j. díez, a. villaverde, f. gebauer, e. rocha, m. dávila, and e. domingo, j. virol. 62:2050-2058, 1988). two nucleotide substitutions, u to c at position -376 and a to g at position -15, (counting as +1 the a of the first functional aug), were fixed within the internal ribosome entry site ... | 1993 | 8389904 |
| conserved tertiary structural elements in the 5' nontranslated region of cardiovirus, aphthovirus and hepatitis a virus rnas. | statistical analyses of rna folding in 5' nontranslated regions (5'ntr) of encephalomyocarditis virus, theiler's murine encephalomyelitis virus, foot-and-mouth disease virus, and hepatitis a virus indicate that two highly significant folding regions occur in the 5' and 3' portions of the 5'ntr. the conserved tertiary structural elements are predicted in the unusual folding regions (ufr) for these viral rnas. the theoretical, common structural elements predicted in the 3' parts of the 5'ntr occur ... | 1993 | 8389442 |
| an indirect sandwich elisa for the identification of bovine enteroviruses. | an indirect sandwich elisa is described for the detection of bovine enteroviruses. the assay was developed as an alternative to the complement fixation test and proved to be more sensitive and convenient. ten bovine enterovirus prototype strains were easily discriminated. no cross-reactions were observed with other picornaviruses including foot-and-mouth disease viruses, swine vesicular disease virus, porcine enteroviruses and bovine rhinovirus. | 1993 | 8388399 |
| design of primers for pcr amplification of highly variable genomes. | a program to aid in the search of primers for specific polymerase chain reaction (pcr) amplification of highly variable genomes is presented. it involves the derivation of variability profiles to identify optimal regions for pcr amplification, taking into account stability of dna-primer hybrids. an application of the program to foot-and-mouth disease virus diagnosis is presented. | 1993 | 8386978 |
| the two species of the foot-and-mouth disease virus leader protein, expressed individually, exhibit the same activities. | initiation of protein synthesis on the foot-and-mouth disease virus rna occurs at two sites, thus, two forms of the leader protein, termed lab and lb, are produced. plasmids have been constructed which encode these proteins either together or individually. plasmids encoding the lab protein alone express a modified form of this protein in which the second methionine residue, which corresponds to the first amino acid of lb, is changed to an alternative residue. four different mutant forms of the l ... | 1993 | 8386879 |
| foot-and-mouth disease virus proteinase 3c inhibits translation in recombinant escherichia coli. | escherichia coli cultures do not survive the expression of recombinant foot-and-mouth disease virus proteinase 3c. this effect is ascribed to degradation of bacterial protein(s), as concluded from the observation of gradual cessation of gene expression upon induction of 3c expression. most likely, translation inhibition is the cause of bacterial death, as (i) cell-free translation of the 3c gene was restored by additional bacterial ribosomes, (ii) ribosomes from proteinase 3c-producing cells dif ... | 1993 | 8386123 |
| structure of a major immunogenic site on foot-and-mouth disease virus. | attachment of foot-and-mouth disease virus (fmdv) to its cellular receptor involves a long and highly antigenic loop containing the conserved sequence, arg-gly-asp, a motif known to be a recognition element in many integrin-dependent cell adhesion processes. in our original crystal structure of fmdv the arg-gly-asp-containing loop ('the loop'), located between beta-strands g and h of capsid protein vp1, was disordered and hence essentially invisible. we previously surmised that its disorder is e ... | 1993 | 8385272 |
| phenotypic and functional characterization of mouse attenuated and virulent variants of foot-and-mouth disease virus type o1 campos. | a series of genetically related variants arising from a parental wild-type isolate of o1 campos and its tissue culture adapted variant were differentiated by various cell culture markers (temperature sensitivity, plaque size, viral yield) and lethality in mice. these isolates were additionally characterized functionally and biochemically by examining poly(c) length, rna synthesis, protein synthesis, and cell receptor binding. in primary bovine kidney cells, the virulent isolates had greater leve ... | 1993 | 8384748 |
| methods used in the structure determination of foot-and-mouth disease virus. | the structure of foot-and-mouth disease virus (fmdv) strain o1 bfs 1860 has been determined to 2.9 a resolution using the molecular-replacement method [acharya, fry, stuart, fox, rowlands & brown (1989). nature (london), 337, 709-716]. crystals of the virus with average dimensions 0.12 x 0.06 x 0.12 mm belong to space group i23, a = 345 a with 1/12 of the icosahedral particle per asymmetric unit giving fivefold noncrystallographic redundancy. oscillation diffraction photographs were collected at ... | 1993 | 8382928 |
| antibody-complexed foot-and-mouth disease virus, but not poliovirus, can infect normally insusceptible cells via the fc receptor. | poliovirus and foot-and-mouth disease virus (fmdv) initiate infection by binding to specific cell surface receptors, which is followed by a poorly understood disassembly process. to probe these early steps of infection, the ability of poliovirus and fmdv to infect cells following binding through an alternative receptor was examined. for these studies, a chinese hamster ovary (cho) cell line expressing the b2 isoform of the murine fc receptor (fcr) was used. both viruses were able to bind to this ... | 1993 | 8380665 |
| comparison of surface properties of picornaviruses: strategies for hiding the receptor site from immune surveillance. | the surface topology and sequence conservation of different picornaviruses have been compared using molecular graphics and statistical analyses. the comparisons suggest that the canyons, surface depressions encircling the fivefold axes, are the sites of receptor attachment of enteroviruses as well as human rhinovirus (hrv). in hrv14, the receptor binding footprint extends beyond the canyon (olson et al. (1993), proc. natl. acad. sci. usa 90, 507-511), but these more exposed regions are not conse ... | 1993 | 8337843 |
| [a rapid solid-phase immunoenzyme method in the diagnosis of viral infections]. | an accelerated solid-phase enzyme-immunoassay has been developed which permits identification of antigens and antibodies to them within 30-40 min, even directly at the site of specimen collection. the method was tested on the models of foot-and-mouth disease virus, vesicular disease of swine, vesicular exanthema of swine, aujeszky's disease, leukemia, and coronavirus infection of cattle. | 1993 | 8303893 |
| avridine and lps from brucella ovis: effect on the memory induced by foot-and-mouth disease virus vaccination in mice. | foot-and-mouth disease is one of the more economically important diseases among meat-producing biungulate species. in contrast to natural infection, current foot-and-mouth disease virus (fmdv) vaccines, prepared with inactivated virus and adjuvants, elicit short-lived protection. the immunomodulating effect on fmdv vaccines of avridine and lipopolysaccharide of brucella ovis (lps) was tested in a murine model. the duration of immunity, protection, stimulation of immunocompetent cells producing a ... | 1993 | 8296482 |
| foot-and-mouth disease virus particles contain replicase protein 3d. | an antibody against the escherichia coli-expressed rna polymerase of foot-and-mouth disease virus (fmdv) reacts with the virus in elisa and radioimmunoprecipitation experiments and with a protein of the disrupted virus particle in an immunoblot analysis. treatment of the virus with trypsin, which cleaves capsid protein vp1 and a 56-kda polypeptide present in trace amount in the particles, reduces the level of the reaction in elisa and radioimmunoprecipitation and eliminates the immunoblot reacti ... | 1994 | 8290591 |
| trans complementation by rna of defective foot-and-mouth disease virus internal ribosome entry site elements. | a region of about 435 bases from the 5' noncoding region of foot-and-mouth disease virus rna directs internal initiation of protein synthesis. this region, termed the internal ribosome entry site (ires), is predicted to contain extensive secondary structure. precise deletion of five predicted secondary structure features has been performed. the mutant ires elements have been constructed into vectors which express bicistronic mrnas and assayed within cells. each of the modified ires elements was ... | 1994 | 8289373 |
| effects of different continium dielectric models in a molecular dynamics and energy minimization study of the antigenic loop of foot-and-mouth disease virus. | this study presents the influence of the dielectric constant on the final structure of the major antigenic loop of the fmdv serotype c. minimizations have been performed on the nine-residue peptide ac-tasargdla-nhme, using two expressions for the dielectric constant: a distance-independent (epsilon = kappa), and a distance-dependent (epsilon = kappa *r) forms, and for kappa values from 1 to 10. in addition, kappa = 40 and 80 has also been considered for the constant expression of the dielectrics ... | 1993 | 8286066 |
| viral antibodies in bovine fetuses in argentina. | in order to establish the prevalence of viral infections of the bovine fetus in argentina, a serological survey for antibodies against viral agents currently affecting cattle in this country was conducted. antibodies against foot-and-mouth disease virus (fmdv), bovine herpesvirus-1 (bhv-1), bovine leukaemia virus (blv), bovine rotavirus (brv), bovine coronavirus (bcv), bovine viral diarrhoea virus (bvdv) and parainfluenza-3 (pi-3) were investigated in a total of 315 fetal serum samples. conventi ... | 1993 | 8284507 |
| co-replication of several isotypes of foot-and-mouth disease virus. | genome segments of the foot-and-mouth disease virus isolates o1lombardy and o3 venezuela that encode, among other products, capsid protein vp1 were amplified using pcr, and the products were cloned and sequenced. the alignment of up to 11 o3-specific sequences revealed six silent nucleotide changes a well as six changes that cause amino acid substitutions in capsid protein vp1 at positions 45, 83, 141, 145, 170 and 178. the heterogeneity of three o1-specific sequences consisted of seven silent e ... | 1993 | 8277282 |
| transient inhibition of foot-and-mouth disease virus infection of bhk-21 cells by antisense oligonucleotides directed against the second functional initiator aug. | the antiviral activity of antisense oligonucleotides corresponding to different regions of foot-and-mouth disease virus (fmdv) genome has been assessed in bhk-21 cells. the locations of the oligonucleotides used were: (i) two regions within the internal ribosome entry site (ires), involved in the regulation of the translation initiation of the viral polyprotein; (ii) each of the two functional initiator augs; (iii) an internal sequence of p2a gene; and (iv) a region at the 3' end non-coding regi ... | 1993 | 8250540 |
| split decomposition: a technique to analyze viral evolution. | a clustering technique allowing a restricted amount of overlapping and based on an abstract theory of coherent decompositions of finite metrics is used to analyze the evolution of foot-and-mouth disease viruses. the emerging picture is compatible with the existence of viral populations with a quasispecies structure and illustrates various forms of evolution of this virus family. in addition, it allows the correlation of these forms with geographic occurrence. | 1993 | 8234292 |
| characterization of the foot-and-mouth disease virus 3c protease expressed in escherichia coli. | we have constructed a clone encoding the foot-and-mouth disease virus (fmdv) 3c protease gene (p3c) using the polymerase chain reaction. the construct was engineered to contain initiation and termination codons and cloned into a plasmid under the control of the bacteriophage t7 promoter. the p3c gene was expressed both in an in vitro transcription-translation system and in vivo in an escherichia coli system containing an inducible t7 rna polymerase gene. in both systems the expressed products we ... | 1993 | 8212567 |
| expression of a foreign protein by influenza a virus. | in this report we describe the rescue of a transfectant influenza a virus which stably expresses a heterologous protein, bacterial chloramphenicol acetyltransferase (cat). the foreign sequences encoding cat are expressed as part of an essential influenza virus segment, that coding for the neuraminidase (na) protein. the novel way by which this was achieved involved inserting in frame the 16-amino-acid self-cleaving 2a protease of foot-and-mouth disease virus between the cat and the na coding seq ... | 1994 | 8207822 |
| antibody response to 146s particle, 12s protein subunit and isolated vp1 polypeptide of foot-and-mouth disease virus type asia-1. | the antibody response to foot-and-mouth disease virus (fmdv) antigens of type asia-1 in guinea-pigs was studied by micro-serum neutralization test (msnt) and enzyme-linked immunosorbent assay (elisa). one inoculation of as little as 1 microgram of binary ethyleneimine (bei)-inactivated 146s virus particles in guinea-pigs elicited enough neutralizing antibodies to protect them against challenge with virulent virus. however, one inoculation of live 146s virus particles elicited higher levels of ne ... | 1994 | 8203119 |
| natural transmission of foot-and-mouth disease virus from african buffalo (syncerus caffer) to cattle in a wildlife area of zimbabwe. | an outbreak of foot-and-mouth disease (fmd) occurred during april 1991 in a trypanosomiasis sentinel cattle herd by the rifa river to the east of lake kariba, zimbabwe. despite the cattle having been vaccinated biannually for the previous five years the disease was severe. the viruses isolated from the affected animals were typed as fmd virus type sat 1. free-living african buffalo (syncerus caffer) which had been using the same watering place as the affected cattle were sampled and fmd type sat ... | 1994 | 8197679 |
| recognition of b and t cell epitopes by cattle immunized with a synthetic peptide containing the major immunogenic site of vp1 fmdv 01 campos. | the precise location of b and t cell epitopes have been established in a peptide containing the major immunogenic site (residues 135-160) of fmdv strain 01 campos (01c) vp1. the peptide (p135-160), administered free or conjugated to bovine serum albumin, induced complete protection in guinea pigs and a strong neutralizing antibody (nab) response in cattle. using a set of partially overlapping peptides it was shown that although several b cell epitopes were distributed along the p135-160, the res ... | 1994 | 8184548 |
| experimental transmission of foot-and-mouth disease virus from carrier african buffalo (syncerus caffer) to cattle in zimbabwe. | four female cattle and three male african buffalo (syncerus caffer) which were free of foot-and-mouth disease (fmd) virus were held together on an island in lake kariba, zimbabwe. the buffalo were experimentally infected with fmd virus type sat2, developed generalised disease and became virus carriers. while the buffalo were in the acute phase of the disease the susceptible contact cattle did not show lesions, no virus was recovered from them and they did not develop serum antibodies. however, f ... | 1994 | 8171808 |
| rapid cell variation can determine the establishment of a persistent viral infection. | evidence for a mechanism of initiation of viral persistence in which the cell, and not the virus, plays a critical role has been obtained using the important animal pathogen foot-and-mouth disease virus (fmdv). we have developed a virulence assay consisting of quantification of the ability of virus to kill cells and of cells to divide in the presence of virus and to initiate a carrier state. cells were cured of fmdv at early times following a cytolytic infection of bhk-21 monolayers with fmdv. w ... | 1994 | 8170973 |
| picornaviral 3c cysteine proteinases have a fold similar to chymotrypsin-like serine proteinases. | the picornavirus family includes several pathogens such as poliovirus, rhinovirus (the major cause of the common cold), hepatitis a virus and the foot-and-mouth disease virus. picornaviral proteins are expressed by direct translation of the genomic rna into a single, large polyprotein precursor. proteolysis of the viral polyprotein into the mature proteins is assured by the viral 3c enzymes, which are cysteine proteinases. here we report the x-ray crystal structure at 2.3 a resolution of the 3c ... | 1994 | 8164744 |
| crystallization and preliminary x-ray diffraction studies of a monoclonal antibody fab fragment against foot-and-mouth disease virus and of its complex with the main antigenic site peptide. | the fab fragment of the neutralizing monoclonal antibody sd6 elicited against foot-and-mouth disease virus (fmdv) c-s8c1 and its complex with a peptide, corresponding to the major antigenic site of fmdv (vp1 residues 136-150, ytasargdlahlttt), have been crystallized using the hanging drop vapor diffusion techniques. for the isolated fab, crystals diffracting to 2.5 a resolution were obtained at room temperature using ammonium sulfate as precipitant. these crystals are monoclinic, space group c2, ... | 1994 | 8159669 |
| a rapid and sensitive chemiluminescence dot-immunobinding assay for screening hybridoma supernatants. | the present report describes a simple and rapid dot-immunobinding assay combined with a chemiluminescence detection system for screening hybridoma supernatants for specific monoclonal antibodies (mabs). small rectangular nitrocellulose filters dotted with either crude mixtures of antigens, or with control samples, were placed in six well plates, incubated with hybridoma supernatants, then stained with peroxidase-conjugated anti-mouse igg. the reaction was performed with a chemiluminescence detec ... | 1994 | 8157996 |
| rgd sequence of foot-and-mouth disease virus is essential for infecting cells via the natural receptor but can be bypassed by an antibody-dependent enhancement pathway. | foot-and-mouth disease virus appears to initiate infection by binding to cells at an arg-gly-asp (rgd) sequence found in the flexible beta g-beta h loop of the viral capsid protein vp1. the role of the rgd sequence in attachment of virus to cells was tested by using synthetic full-length viral rnas mutated within or near the rgd sequence. baby hamster kidney (bhk) cells transfected with three different rnas carrying mutations bordering the rgd sequence produced infectious viruses with wild-type ... | 1994 | 8127909 |
| genetic relationships between foot-and-mouth disease type asia 1 viruses. | the sequence of 165 nucleotides at the 3' end of the 1d (vp1) gene of foot-and-mouth disease (fmd) virus was determined for 44 type asia 1 strains isolated from throughout asia between 1954-92. analysis of the relationships between the virus genomes showed epidemiological links not previously evident. the possible origin of the only outbreak of fmd asia 1 to have occurred in europe, in greece in 1984, was identified because the nucleotide sequence of this virus was closely-related to the sequenc ... | 1994 | 8119360 |
| analysis of mixed foot-and-mouth disease virus infections in saudi arabia: prolonged circulation of an exotic serotype. | plaque purification of foot-and-mouth disease (fmd) type o viruses isolated from cattle in saudi arabia showed the presence of mixed serotype infections. sixteen out of 31 samples collected between 1985 and 1991 also contained asia 1 virus, a serotype which had previously only been isolated from a single outbreak in that country in 1980. nucleotide sequences of the asia 1 component of all these samples revealed little variation and showed that they were closely related to both a russian lapinize ... | 1994 | 8119359 |
| foot-and-mouth disease virus 2a oligopeptide mediated cleavage of an artificial polyprotein. | we describe the construction of a plasmid (pcat2agus) encoding a polyprotein in which a 19 amino acid sequence spanning the 2a region of the foot-and-mouth disease virus (fmdv) polyprotein was inserted between the reporter genes chloramphenicol acetyl transferase (cat) and beta-glucuronidase (gus) maintaining a single, long open reading frame. analysis of translation reactions programmed by this construct showed that the inserted fmdv sequence functioned in a manner similar to that observed in f ... | 1994 | 8112307 |
| antigenic heterogeneity of a foot-and-mouth disease virus serotype in the field is mediated by very limited sequence variation at several antigenic sites. | antigenic variation in a major discontinuous site (site d) of foot-and-mouth disease virus (fmdv) of serotype c has been evaluated with neutralizing monoclonal antibodies. isolates representing the major evolutionary sublines previously defined for serotype c were compared. extensive variation, comparable to that of continuous epitopes within the hypervariable immunodominant site a (the vp1 g-h loop), was found. the amino acid sequences of the complete capsids of three antigenically highly diver ... | 1994 | 8107204 |
| experiments on an early protection against foot-and-mouth disease virus. | the influence of the peptide diacetylsplenopentin (sp5) on an early protection of guinea pigs against foot-and-mouth disease (fmd) was investigated. 80% protection was achieved if sp5 was applied in a dose of 2 mg one day before challenge with fmd virus (fmdv) type o1 lausanne strain. in comparison with this a conventional commercial adsorbate vaccine protected guinea pigs about 7 days after vaccination. an earlier protection can be obtained in general by vaccination with a higher content of the ... | 1993 | 8105663 |
| application of monoclonal antibodies to quality control of foot-and-mouth disease vaccines. | panels of monoclonal antibodies (mabs) produced against foot-and-mouth disease (fmd) virus types o, a and c were selected for cell culture neutralization titre (nt), mouse protection index (mpi), trypsin sensitivity (ts) and avidity to different epitopes. the selected sets were used to assay the antigen concentration and the fit between fmdv vaccine and challenge strains. it was observed that fmd vaccines protect more than 75% of vaccinated cattle when manufactured with antigens characterized by ... | 1994 | 8091844 |
| the structure and antigenicity of a type c foot-and-mouth disease virus. | picornaviruses are responsible for a wide range of mammalian diseases and, in common with other rna viruses, show considerable antigenic variation. foot-and-mouth disease viruses (fmdvs) constitute one genus of the picornavirus family and are classified into seven serotypes, each of which shows considerable intratypic variation. this antigenic variation leads to continuing difficulties in controlling the disease. to date the structure of only one serotype, o, has been reported. | 1994 | 8081743 |
| genetic variation of foot-and-mouth disease virus from field outbreaks to laboratory isolation. | foot-and-mouth disease virus (fmdv), by nature of its rna genome, possesses a high rate of mutation during replication. this results in extensive genetic polymorphism of virus populations in nature. the emergence of fmdv variants during replication has been reported. genetic changes in the viral capsid protein (vp1) gene can result in amino acid changes affecting the immunodominant epitopes of fmdv. the genetic heterogeneity of fmdv in the field and the antigenic variants observed after cell cul ... | 1994 | 8079512 |
| circular dichroism, molecular modeling, and serology indicate that the structural basis of antigenic variation in foot-and-mouth disease virus is alpha-helix formation. | seven antigenic variants obtained from a single field isolate of foot-and-mouth disease virus, serotype a12, differ only at residues 148 and 153 in the immunodominant loop of viral protein vp1. synthetic peptides corresponding to the region 141-160 are highly immunogenic. uv circular dichroism shows that (i) in aqueous solution the peptides are nearly identical, but in 100% trifluoroethanol they display helix-forming properties which correlate well with their serological crossreactivities for an ... | 1994 | 8078900 |
| sequence of the s fragment of foot-and-mouth disease virus type a12. | the foot-and-mouth disease virus (fmdv) genome contains a 5' untranslated region (s fragment) capable of forming a stem-loop structure of over 350 bases, which is separated from the remainder of the genome by a homopolymeric cytidylic acid tract (poly(c)) of variable length. the sequence of the s fragment of serotype a12 appears more similar to those of type o1 or type c3 than to subtype a10. the relatively large difference between the s fragment sequences of two type a viruses suggests that the ... | 1994 | 8073639 |
| direct pcr detection of foot-and-mouth disease virus. | a pcr assay for the detection and characterization of foot-and-mouth disease virus was developed. the procedure allows rt-pcr amplification following direct adsorption of viral suspensions to microtiter plates, avoiding previous steps of phenol-extraction or heating. using this procedure, fmdv-specific (based on 3d gene sequences), as well as serotype-specific (based on vp1 gene sequences) amplification were achieved for viral samples of serotypes a, o and c, either from cell culture supernatant ... | 1994 | 8071421 |
| dituftsin and polytuftsin induce an anti-peptide igg response to non-immunogenic peptides in mice. | the effect of covalently attaching multiple forms of the immunomodulating tetrapeptide tuftsin to normally non-immunogenic peptides was studied in balb/c and c57bl/6 mice. the peptides: (nanp)3 from the plasmodium falciparum circumsporozoite protein and peptides 136-152 and 205-213 derived from the capsid protein of foot-and-mouth disease virus were coupled to polytuftsin or dituftsin. anti-peptide igg titers were determined after two immunizations. all of these three non-immunogenic peptides co ... | 1994 | 8070848 |
| foot-and-mouth disease virus leader proteinase: purification of the lb form and determination of its cleavage site on eif-4 gamma. | many picornaviruses cause a dramatic decrease in the translation of cellular mrnas in the infected cell, without affecting the translation of their own rna. specific proteolysis of protein synthesis initiation factor eif-4 gamma occurs during infection with rhinoviruses, enteroviruses, and aphthoviruses, apparently leading to an inability of the ribosomes to bind capped mrnas. cleavage of eif-4 gamma in human rhinoviruses and enteroviruses is carried out by the viral 2a proteinase; in aphthoviru ... | 1994 | 8057448 |
| induction of effective cross-reactive immunity by fmdv peptides is critically dependent upon specific mhc-peptide-t cell interactions. | bocd4+ t-cell clones specific for a peptide derived from foot-and-mouth disease virus envelope protein, vp1 (fmdv15) were generated from two responder cattle. one animal was a high and the other was an intermediate responder in terms of both t-cell and antibody responses. however both animals had identical major histocompatibility complex (mhc) class ii dr-like types (drbf3,6) according to a one-dimensional isoelectric focusing method which distinguishes dr-like alleles. in contrast, mixed lymph ... | 1994 | 8045586 |
| a comparative study of serological and biochemical methods for strain differentiation of foot-and-mouth disease type a viruses. | three serological and three biochemical methods were used to compare five field isolates of foot-and-mouth disease virus (fmdv) from western india with nine reference vaccine strains and five field isolates from other countries. the serological tests (liquid-phase elisa and virus neutralization) were able to distinguish between the three reference vaccine strains examined, but the five indian field isolates reacted poorly with antisera produced against these vaccine strains. analysis of monoclon ... | 1994 | 8042276 |
| modelling the spread of foot-and-mouth disease virus. | foot-and-mouth disease is an economically important viral disease in animals. it is shown that airborne diffusion is one of the main sources of contamination between animals and between herds. epidemiological data linked to viral particle excretion can thus be used in a predictive model, added to meteorological data related to the few days before the slaughter of animals. the model computes, on a 10 km radius around the outbreak and in every space direction, the quantity of viral particles that ... | 1994 | 8038801 |
| ompa-fmdv vp1 fusion proteins: production, cell surface exposure and immune responses to the major antigenic domain of foot-and-mouth disease virus. | exposure at the bacterial outer surface of the major antigenic epitope of the foot-and-mouth disease (fmdv) viral protein vp1 was studied using protein fusion with outer membrane protein a (ompa) of shigella dysenteriae for production and transport of the foreign polypeptide to the outer membrane of escherichia coli. fusion constructs with vp1 peptide insertions of up to 56 amino acids in the third outer domain of ompa could be demonstrated on the bacterial surface by indirect immunofluorescence ... | 1994 | 8036821 |
| animal-derived antigenic variants of foot-and-mouth disease virus type a12 have low affinity for cells in culture. | we recently have shown that binding of foot-and-mouth disease virus (fmdv) to cells in culture requires an arginine-glycine-aspartic acid (rgd) sequence in the g-h loop of the capsid protein vp1 (p. w. mason, e. rieder, and b. baxt, proc. natl. acad. sci. usa 91:1932-1936, 1994). in this report, we show that fmdv type a12 viruses found in infected bovine tongue tissue (btt) differ from their tissue culture-grown derivatives at amino acid residues near the rgd. viruses genetically engineered to c ... | 1994 | 8035529 |
| comparison of liquid-phase and mab-blocking elisa for assessment of the reactivity of monoclonal antibodies to foot-and-mouth disease virus. | 1994 | 8034975 | |
| the structure of an immunodominant loop on foot and mouth disease virus, serotype o1, determined under reducing conditions. | residues 136-159 of vpi of foot and mouth disease virus (fmdv) comprise the g-h loop of the protein and form a prominent feature on the surface of virus particles. this sequence contains an immunodominant neutralizing epitope, which can be mimicked with synthetic peptides, and includes an arg, gly, asp motif which has been implicated in the binding of the virus to cellular receptors. crystallographic analysis of native virus particles failed to resolve the structure of this region due to its dis ... | 1994 | 8032279 |
| analysis of sites of foot and mouth disease virus persistence in carrier cattle via the polymerase chain reaction. | this study was undertaken in order to explore possible sites of foot-and-mouth disease virus (fmdv) persistence during the carrier state. tissue samples taken from experimentally infected animals at different times post-infection (p.i.) were examined by conventional viral isolation and the polymerase chain reaction (pcr) technique. the analysis of samples from several organs taken from 17 bovines between 3 and 270 days p.i. allowed the following conclusions: 1) virus present in oesophageal-phary ... | 1994 | 8031235 |
| development of cowpea mosaic virus as a high-yielding system for the presentation of foreign peptides. | it has recently been shown that cowpea plants can be infected with a cowpea mosaic virus (cpmv) chimera containing an antigenic site from foot-and-mouth disease virus (usha et al., virology 197, 366-374, 1993). analysis of progeny rna produced during such an infection has revealed that the inserted sequence is rapidly lost during serial passaging, probably by a process of homologous recombination. using the information gained from this analysis, we have redesigned the chimeras in such a way that ... | 1994 | 8030255 |
| efficient use of lactose for the lac promoter-controlled overexpression of the main antigenic protein of the foot and mouth disease virus in escherichia coli under fed-batch fermentation conditions. | derivatives of the lac promoter (tac, pac, rac) belong to the strongest bacterial promoters which are frequently used for the induced overexpression of foreign genes in escherichia coli. however, their use in fermentation processes is strongly restricted because of the high cost of the inducer iso-propyl-beta-d-thiogalactopyranoside (iptg). the aim of this work was to investigate the possibility of using lac-derived promoters in high cell density processes resulting in a high yield of the induce ... | 1994 | 8011364 |
| [synthesis of new fragments of vp1 protein fragments from foot and mouth disease virus type a22. synthesis of fragments 134-139, 134-145, 140-145, 150-155, and 150-159]. | fragments 134-145 and 150-159 of the antigenic-region of the vp1 protein of the a22 foot-and-mouth disease virus were synthesized by classic methods of peptide chemistry with isobutyl chloroformate as a coupling reagent. after purification by hplg and amino acid analysis, the free peptides h-gly-lys-tyr-ser-ala-gly-gly-leu-gly-arg-arg-gly-oh and h-leu-ala-ala-arg-val-ala-lys-gln-leu-pro-oh were conjugated with bsa by means of n,n-dicyclohexylcarbodiimide. the conjugates were used, with complete ... | 1994 | 8003043 |
| immunogenicity of non-structural proteins of foot-and-mouth disease virus: differences between infected and vaccinated swine. | non-structural as well as vp1 recombinant proteins of foot-and-mouth disease virus (fmdv) produced in e. coli, have been used to study the specific antibody response of infected or vaccinated swine. an analysis of sera from infected pigs, using a direct elisa, showed that polypeptide 3abc (spanning non-structural proteins 3a, 3b and 3c) was the most antigenic among the recombinant proteins studied and allowed specific detection of fmdv infected swine from the second week after the infection. the ... | 1994 | 8002780 |
| insertion of a 27 amino acid viral peptide in different zones of escherichia coli beta-galactosidase: effects on the enzyme activity. | seven internal, putatively exposed regions of escherichia coli beta-galactosidase have been explored regarding their tolerance to insertions of large foreign peptides. small sequence modifications, including amino acid substitutions and small deletions, were introduced into the lacz gene to generate unique bamhi restriction sites. by using these mutant genes, a 27 amino acid stretch reproducing the hypervariable loop of foot-and-mouth disease virus vp1 protein (site a) was further inserted in pr ... | 1994 | 7988875 |
| effect of adjuvant formulations on the induction of virus-neutralizing and virus-binding antibodies by chemosynthetic peptides of vp1 of foot-and-mouth-disease virus. | synthetic peptides corresponding to the 141-160 amino acid sequence of the protein vp1 of virus type o1 kaufbeuren (o1k) and a5 riems (a5r) were conjugated to thyroglobulin and mixed with complete freund's adjuvant (cfa) or incomplete freund's adjuvant (ifa) together with quil a. although the peptide of a5r, together with ifa and quil a, or with cfa, elicited a high antibody response to the virus in the elisa, formula containing both ifa and quil a induced only high titres of virus-neutralizing ... | 1994 | 7985430 |