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how significant to plant n nutrition is the direct consumption of soil microbes by roots?the high degree to which plant roots compete with soil microbes for organic forms of nitrogen (n) is becoming increasingly apparent. this has culminated in the finding that plants may consume soil microbes as a source of n, but the functional significance of this process remains unknown. we used (15) n- and (14) c-labelled cultures of soil bacteria to measure rates of acquisition of microbes by sterile wheat roots and plants growing in soil. we compared these rates with acquisition of (15) n del ...201323718181
mixed production of filamentous fungal spores for preventing soil-transmitted helminth zoonoses: a preliminary analysis.helminth zoonoses are parasitic infections shared by humans and animals, being the soil-transmitted helminths (sths) mainly caused by roundworms (ascarids) and hookworms. this study was aimed to assess the individual and/or mixed production of two helminth-antagonistic fungi, one ovicide (mucor circinelloides) and other predator (duddingtonia flagrans). fungi were grown both in petri plates and in a submerged culture (composed by water, nacl, na2hpo4 · 12 h2o, and wheat (triticum aestivum)). a f ...201323710451
isohydric and anisohydric strategies of wheat genotypes under osmotic stress: biosynthesis and function of aba in stress responses.changes in water potential (ψw), stomatal conductance, abscisic acid (aba) accumulation, expression of the major genes involved in aba biosynthesis, activities of abscisic aldehyde oxidase (ao, ec 1.2.3.1) and antioxidant enzymes were studied in two wheat cultivars with contrasting acclimation strategies subjected to medium strength osmotic stress (-0.976mpa) induced by polyethylene glycol (peg 6000). because the biosynthetic pathway of aba involves multiple gene products, the aim of this study ...201323702247
evidence for adaptive evolution of low-temperature stress response genes in a pooideae grass ancestor.adaptation to temperate environments is common in the grass subfamily pooideae, suggesting an ancestral origin of cold climate adaptation. here, we investigated substitution rates of genes involved in low-temperature-induced (lti) stress responses to test the hypothesis that adaptive molecular evolution of lti pathway genes was important for pooideae evolution. substitution rates and signatures of positive selection were analyzed using 4330 gene trees including three warm climate-adapted species ...201323701123
[optimized condition for protoplast isolation from maize, wheat and rice leaves].maize (zea mays l.), wheat (triticum aestivum l.) and rice (oryza sativa l.) are three staple crops and accordingly it is very meaningful to optimize the condition of their protoplasts isolation. the concentration of the enzyme, the time of isolation and centrifugal force in protoplast isolation were investigated to find their effects on protoplast yield and viability using leaves of maize (zong 3), wheat (chinese spring) and rice (nipponbare). the results show that the concentration of the enzy ...201323697167
impact of biotic and abiotic stresses on the competitive ability of multiple herbicide resistant wild oat (avena fatua).ecological theory predicts that fitness costs of herbicide resistance should lead to the reduced relative abundance of resistant populations upon the cessation of herbicide use. this greenhouse research investigated the potential fitness costs of two multiple herbicide resistant (mhr) wild oat (avena fatua) populations, an economically important weed that affects cereal and pulse crop production in the northern great plains of north america. we compared the competitive ability of two mhr and two ...201323696896
overexpression of three taexpa1 homoeologous genes with distinct expression divergence in hexaploid wheat exhibit functional retention in arabidopsis.common wheat is a hexaploid species with most of the genes present as triplicate homoeologs. expression divergences of homoeologs are frequently observed in wheat as well as in other polyploid plants. however, little is known about functional variances among homologous genes arising from polyploidy. expansins play diverse roles in plant developmental processes related to the action of cell wall loosening. expression of the three taexpa1 homoeologs varied dynamically at different stages and organ ...201323696842
diversity analysis of biocontrol bacillus isolated from rhizospheric soil of rice-wheat (oryza sativa-triticum aestivum l.) at india. 201323695416
molecular mapping of leaf rust resistance gene lrnj97 in chinese wheat line neijiang 977671.neijiang 977671 and 19 near-isogenic lines with known leaf rust resistance genes were inoculated with 12 pathotypes of puccinia triticina for postulation of leaf rust resistance genes effective at the seedling stage. the reaction pattern of neijiang 977671 differed from those of the lines with known leaf rust resistance genes used in the test, indicating that neijiang 977671 may carry a new leaf rust resistance gene(s). with the objective of identifying and mapping the new gene for resistance to ...201323689746
identification of a novel gene, h34, in wheat using recombinant inbred lines and single nucleotide polymorphism markers.hessian fly (hf), mayetiola destructor, is an important pest of wheat (triticum aestivum l.) worldwide. because it has multiple biotypes that are virulent to different wheat hf resistance genes, pyramiding multiple resistance genes in a cultivar can improve resistance durability, and finding dna markers tightly linked to these genes is essential to this process. this study identified quantitative trait loci (qtls) for hessian fly resistance (hfr) in the wheat cultivar 'clark' and tightly linked ...201323689741
planex: the plant co-expression database.the plant co-expression database (planex) is a new internet-based database for plant gene analysis. planex (http://planex.plantbioinformatics.org) contains publicly available genechip data obtained from the gene expression omnibus (geo) of the national center for biotechnology information (ncbi). planex is a genome-wide co-expression database, which allows for the functional identification of genes from a wide variety of experimental designs. it can be used for the characterization of genes for ...201323688397
comparative analysis of the chloroplast proteomes of a wheat (triticum aestivum l.) single seed descent line and its parents.to understand the photosynthetic basis in a single seed descent line 10 (ssdl10) of wheat contained high atp in leaves, the chloroplast proteome was compared to ssdl10 and its parents using a combination of 2-de and maldi-tof ms and ms/ms. more than 300 protein spots could be reproducibly detected in the 2d gel. 18 spots were differentially expressed between ssdl10 and the parents, 16 of which were identified by ms with the localization in chloroplasts. these proteins are grouped into diverse fu ...201323683508
fusarium damage in cereal grains from western canada. 1. phylogenetic analysis of moniliformin-producing fusarium species and their natural occurrence in mycotoxin-contaminated wheat, oats, and rye.harvest samples of common wheat (triticum aestivum), oats (avena sativa), and rye (secale cereale) from producers in western canada were analyzed for fungal infection by toxigenic fusarium species and contamination by trichothecenes and moniliformin (mon). fusarium graminearum and f. avenaceum were the two most frequently isolated species from samples of rye and wheat collected in 2010. f. poae and f. sporotrichioides were more commonly detected in randomly selected oat seeds. other toxigenic fu ...201323683177
a wheat pi4k gene whose product possesses threonine autophophorylation activity confers tolerance to drought and salt in arabidopsis.phosphoinositides are involved in regulation of recruitment and activity of signalling proteins in cell membranes. phosphatidylinositol (pi) 4-kinases (pi4ks) generate pi4-phosphate the precursor of regulatory phosphoinositides. no type ii pi4k research on the abiotic stress response has previously been reported in plants. a stress-inducible type ii pi4k gene, named tapi4kiiγ, was obtained by de novo transcriptome sequencing of drought-treated wheat (triticum aestivum). tapi4kiiγ, localized on t ...201323682116
effect of consumption of micronutrient enriched wheat steamed bread on postprandial plasma glucose in healthy and type 2 diabetic subjects.steamed wheat bread have previously been shown to induce comparatively high postprandial plasma glucose responses, on the contrary, buckwheat products induced lower postprandial plasma glucose. the present study was to assess the effects of micronutrient enriched bread wheat variety jizi439 and buckwheat on postprandial plasma glucose in healthy and diabetic subjects comparing with buckwheat and other bread wheat varieties.201323680007
haplotype analysis of qtls attributed to salinity tolerance in wheat (triticum aestivum).a diverse collection of wheat germplasm, consisting of 100 bread wheat lines with varying levels of salinity tolerance were evaluated based on incomplete block design (lattice) with two replications in field conditions. plant material was screened for salinity tolerance under normal and saline field conditions. subsequently in order to assess the haplotype diversity of qtls attributed to salinity tolerance in wheat (triticum aestivum), a collection of 30 extremes tolerant and sensitive genotypes ...201323677711
relevance for food sciences of quantitative spatially resolved element profile investigations in wheat (triticum aestivum) grain.bulk element concentrations of whole grain and element spatial distributions at the tissue level were investigated in wheat (triticum aestivum) grain grown in zn-enriched soil. inductively coupled plasma mass spectrometry and inductively coupled plasma optical emission spectrometry were used for bulk analysis, whereas micro-proton-induced x-ray emission was used to resolve the two-dimensional localization of the elements. soil zn application did not significantly affect the grain yield, but did ...201323676898
ectopic expression of a wheat wrky transcription factor gene tawrky71-1 results in hyponastic leaves in arabidopsis thaliana.leaf type is an important trait that closely associates with crop yield. wrky transcription factors exert diverse regulatory effects in plants, but their roles in the determination of leaf type have not been reported so far. in this work, we isolated a wrky transcription factor gene tawrky71-1 from a wheat introgression line sr3, which has larger leaves, superior growth capacity and higher yield than its parent common wheat jn177. tawrky71-1 specifically expressed in leaves, and produced more mr ...201323671653
[development and study of spring bread wheat variety pamyati maystrenko with introgression of genetic material from synthetic hexaploid triticum timopheevii zhuk. x aegilops tauschii coss].synthetic hexaploids are bridges for transferring new genes that determine resistance to stress factors from wild-type species to bread wheat. in the present work, the method of developing the spring bread wheat variety pamyati maystrenko and the results of its study are described. this variety was obtained using one of the immune lines produced earlier via the hybridization of the spring bread wheat variety saratovskaya 29 with the synthetic hexaploid t. timopheevii zhuk. x ae. tauschii coss. t ...201323662428
a projection of ozone-induced wheat production loss in china and india for the years 2000 and 2020 with exposure-based and flux-based approaches.using a high-resolution (40 × 40 km) chemical transport model coupled with the regional emission inventory in asia (reas), we simulated surface ozone concentrations ([o3 ]) and evaluated o3 -induced wheat production loss in china and india for the years 2000 and 2020 using dose-response functions based on aot40 (accumulated [o3 ] above 40 ppb) and pody (phytotoxic o3 dose, accumulated stomatal flux of o3 above a threshold of y nmol m(-2) s(-1) ). two o3 dose metrics (90 days aot40 and pod6 ) wer ...201323661338
fine mapping taflw1, a major qtl controlling flag leaf width in bread wheat (triticum aestivum l.).flag leaf width (flw) is directly related to photosynthetic capacity and yield potential in wheat. in a previous study, qflw.nau-5a controlling flw was detected on chromosome 5a in the interval possessing fhb5 for type i fusarium head blight (fhb) resistance using a recombinant inbred line population derived from nanda2419 × wangshuibai.201323661078
whole-genome mapping of agronomic and metabolic traits to identify novel quantitative trait loci in bread wheat grown in a water-limited environment.drought is a major environmental constraint responsible for grain yield losses of bread wheat (triticum aestivum) in many parts of the world. progress in breeding to improve complex multigene traits, such as drought stress tolerance, has been limited by high sensitivity to environmental factors, low trait heritability, and the complexity and size of the hexaploid wheat genome. in order to obtain further insight into genetic factors that affect yield under drought, we measured the abundance of 20 ...201323660834
uv-screening of grasses by plant silica layer?uv-screening by terrestrial plants is a crucial trait since colonization of terrestrial environments has started. in general, it is enabled by phenolic substances. especially for grasses it remains unclear why plants grown under the absence of uv-b-radiation exhibit nonetheless a high uv-b-screening potential. but this may be explained by the uv-screening effect of the silicon double layer. it was shown for seedlings of soybeans (glycine max l.) and wheat (triticum aestivum l.) that enhanced sil ...201323660676
dbwfa: a web-based database for functional annotation of triticum aestivum transcripts.the functional annotation of genes based on sequence homology with genes from model species genomes is time-consuming because it is necessary to mine several unrelated databases. the aim of the present work was to develop a functional annotation database for common wheat triticum aestivum (l.). the database, named dbwfa, is based on the reference ncbi unigene set, an expressed gene catalogue built by expressed sequence tag clustering, and on full-length coding sequences retrieved from the trifld ...201323660284
genomic and chromosomal distribution patterns of various repeated dna sequences in wheat revealed by a fluorescence in situ hybridization procedure.wheat (triticum aestivum l.) is an allohexaploid, in which each of the three genomes has a high 1c content. this indicates the presence of multiple tandemly repeated sequences, which should be detectable using in situ hybridization. some repeats have already been described, but others remain to be recognized. to discover others, 2000 plasmid wheat clones were examined for signal presence after fluorescence in situ hybridization and microscopic signal observation. among them, 47 clones produced s ...201323659696
ga-responsive dwarfing gene rht12 affects the developmental and agronomic traits in common bread wheat.opportunities exist for replacing reduced height (rht) genes rht-b1b and rht-d1b with alternative dwarfing genes, such as the gibberellin-responsive gene rht12, for bread wheat improvement. however, a comprehensive understanding of the effects and mode of action of rht12 is lacking. in the present study, the effects of rht12 were characterized by analyzing its effects on seeding vigour, seedling roots, leaf and stem morphology, spike development and carbohydrate assimilation and distribution. th ...201323658622
effect of maturity at harvest on yield, chemical composition, and in situ degradability for annual cereals used for swath grazing.the objective of this study was to determine how harvest maturity of whole-crop cereals commonly used in swath grazing systems in western canada affects yield, chemical composition, and in situ digestibility. we hypothesized that the increase in yield with advancing maturity would not offset the decline in digestibility and, thus, the yield of effectively degradable dm (eddm) would decline with advanced stages of maturity. four replicate plots of barley (hordeum vulgare l.; cv. cdc cowboy), mill ...201323658356
earliness per se qtls and their interaction with the photoperiod insensitive allele ppd-d1a in the cutler × ac barrie spring wheat population.earliness per se regulates flowering time independent of environmental signals and helps to fine tune the time of flowering and maturity. in this study, we aimed to map earliness per se quantitative trait loci (qtls) affecting days to flowering and maturity in a population developed by crossing two spring wheat cultivars, cutler and ac barrie. the population of 177 recombinant inbred lines (rils) was genotyped for a total of 488 ssr and dart polymorphic markers on all 21 chromosomes. three qtls ...201323649650
identification and mapping in spring wheat of genetic factors controlling stem rust resistance and the study of their epistatic interactions across multiple environments.stem rust (puccinia graminis f. sp. tritici) is responsible for major production losses in hexaploid wheat (triticum aestivum l.) around the world. the spread of stem rust race ug99 and variants is a threat to worldwide wheat production and efforts are ongoing to identify and incorporate resistance. the objectives of this research were to identify quantitative trait loci (qtl) and to study their epistatic interactions for stem rust resistance in a population derived from the canadian wheat culti ...201323649649
protective role of a methanolic extract of spinach (spinacia oleracea l.) against pb toxicity in wheat (triticum aestivum l.) seedlings: beneficial effects for a plant of a nutraceutical used with animals.spinach extracts contain powerful natural antioxidants and have been used to improve the response of animal cells to various stress factors. the aim of the present study was to assess the effects of a methanolic extract of spinach (se) used at two concentrations (21.7 and 217 ppm) on the growth, certain enzymes and antioxidant systems in wheat seedlings under lead stress. when wheat seedlings were grown for 7 days in a solution containing pb(no3)2 (3 mm), germination and growth were impaired, wh ...201323645001
isolation of a wheat (triticum aestivum l.) mutant in aba 8'-hydroxylase gene: effect of reduced aba catabolism on germination inhibition under field condition.pre-harvest sprouting, the germination of mature seeds on the mother plant under moist condition, is a serious problem in cereals. to investigate the effect of reduced abscisic acid (aba) catabolism on germination in hexaploid wheat (triticum aestivum l.), we cloned the wheat aba 8'-hydroxyase gene which was highly expressed during seed development (taaba8'oh1) and screened for mutations that lead to reduced aba catabolism. in a screen for natural variation, one insertion mutation in exon 5 of t ...023641187
cloning of allene oxide cyclase gene from leymus mollis and analysis of its expression in wheat-leymus chromosome addition lines.leymus mollis (triticeae; poaceae) is a useful genetic resource for wheat (triticum aestivum l.) breeding via wide hybridization to introduce its chromosomes and integrate its useful traits into wheat. leymus mollis is highly tolerant to abiotic stresses such as drought and salinity and resistant to various diseases, but the genetic mechanisms controlling its physiological tolerance remain largely unexplored. we identified and cloned an allene oxide cyclase (aoc) gene from l. mollis that was str ...201323641183
a major quantitative trait locus for cold-responsive gene expression is linked to frost-resistance gene fr-a2 in common wheat.low temperature induces expression of cor (cold-responsive)/lea (late embryogenesis-abundant) gene family members through c-repeat binding factor (cbf) transcription factors in common wheat. however, the relationship between the genetic loci controlling cold-responsive gene expression and freezing tolerance is unclear. in expression quantitative trait locus (eqtl) analysis, accumulated transcripts of cor/lea and cbf genes were quantified in recombinant inbred lines derived from a cross between t ...201323641182
genetic dissection reveals effects of interaction between high-molecular-weight glutenin subunits and waxy alleles on dough-mixing properties in common wheat.the glutenin and waxy loci of wheat are important determinants of dough quality. this study was conducted to evaluate the effects of high-molecular-weight glutenin (hmw-gs) and waxy alleles on dough-mixing properties. molecular mapping was used to investigate these effects on mixograph properties in a population of 290 (nuomai1 x gaocheng8901) recombinant inbred lines (rils) from three environments in the harvest years 2008, 2009 and 2011. the results indicated the following: (i) the glu-a1 and ...201323640407
cloning and characterization of tasnrk2.3, a novel snrk2 gene in common wheat.environmental stresses such as drought, salinity, and cold are major adverse factors that significantly affect agricultural productivity. protein phosphorylation/dephosphorylation is a major signalling event induced by osmotic stress in higher plants. sucrose non-fermenting 1-related protein kinase 2 (snrk2) family members play essential roles in the response to hyperosmotic stresses in plants. in this study, the tasnrk2.3 gene, a novel snrk2 member was cloned, and three copies located on chromo ...201323630328
fast-tracking development of homozygous transgenic cereal lines using a simple and highly flexible real-time pcr assay.a crucial step in the evaluation of newly produced transgenic plants is the selection of homozygous plants. here we describe an efficient and highly flexible real-time pcr-based method for the development of homozygous lines in plant models with complex (multiple) genomes and/or relatively long generation times (>3 months) using direct copy number determinations.201323627847
the root cortex cell hydraulic conductivity is enhanced with increasing chromosome ploidy in wheat.wheat (triticum spp.) root water uptake is enhanced with increasing chromosome ploidy, but the underlying mechanism is unclear. the leaf transpiration rate (e), individual root (lp(r)) and cortical cell (lp(c)) hydraulic conductivity, cortical cell volume (v(c)) and transcription levels of two putative plasma intrinsic aquaporin genes (pips) were investigated in wheat seedlings with different chromosome ploidy (triticum monococcum (2x, aa); triticum dicoccum (4x, bb); triticum aestivum (6x, aabb ...201323624112
structural and physiological characterization during wheat pericarp development.the cytological and physiological features of developing wheat pericarp were clearly characterized in this report. our results may be helpful to articulate the functions of pericarp during the seed development. although wheat pericarp has been well studied, knowledge of the sequence of events in the process of pericarp development is incomplete. in the present study, the structural development process of wheat (triticum aestivum l.) pericarp was investigated in detail using resin microtomy and m ...201323615695
introducing a new breed of wine yeast: interspecific hybridisation between a commercial saccharomyces cerevisiae wine yeast and saccharomyces mikatae.interspecific hybrids are commonplace in agriculture and horticulture; bread wheat and grapefruit are but two examples. the benefits derived from interspecific hybridisation include the potential of generating advantageous transgressive phenotypes. this paper describes the generation of a new breed of wine yeast by interspecific hybridisation between a commercial saccharomyces cerevisiae wine yeast strain and saccharomyces mikatae, a species hitherto not associated with industrial fermentation e ...201323614011
physical mapping integrated with syntenic analysis to characterize the gene space of the long arm of wheat chromosome 1a.bread wheat (triticum aestivum l.) is one of the most important crops worldwide and its production faces pressing challenges, the solution of which demands genome information. however, the large, highly repetitive hexaploid wheat genome has been considered intractable to standard sequencing approaches. therefore the international wheat genome sequencing consortium (iwgsc) proposes to map and sequence the genome on a chromosome-by-chromosome basis.201323613713
ability of leaf mesophyll to retain potassium correlates with salinity tolerance in wheat and barley.this work investigated the importance of the ability of leaf mesophyll cells to control k(+) flux across the plasma membrane as a trait conferring tissue tolerance mechanism in plants grown under saline conditions. four wheat (triticum aestivum and triticum turgidum) and four barley (hordeum vulgare) genotypes contrasting in their salinity tolerance were grown under glasshouse conditions. seven to 10-day-old leaves were excised, and net k(+) and h(+) fluxes were measured from either epidermal or ...023611560
in silico 3d structure prediction and hydrogen peroxide binding study of wheat catalase.catalase (cat) is one of the most active enzyme catalysts found in plants, animals and in all aerobic microorganisms. the major function of the enzyme is to decompose h2o2, produced by cellular metabolic activities under normal and stressful conditions to water and oxygen. the present study involves 3d structure modeling of wheat catalase from triticum aestivum by modeller9v7 and its binding study with h2o2. the evaluation of the model was based on discrete optimized protein energy score (dope). ...201323605643
comparative physiological response of wheat genotypes under terminal heat stress.wheat (triticum aestivum l.), a staple food crop, is of great commercial importance. its production is restricted due to multiple environmental stresses. there are indications that the wheat production is consistently limited by terminal heat stress. previous studies revealed a varied response of different wheat genotypes under heat stress conditions. here, comparative physiological changes in wheat genotypes viz., dbw-140, raj-3765, pbw-574, k-0-307 and hs-240 were evaluated under timely and la ...201323603954
characterization of γ-aminobutyric acid metabolism and oxidative damage in wheat (triticum aestivum l.) seedlings under salt and osmotic stress.the molecular response of plants to abiotic stresses has been considered a process mainly involved in the modulation of transcriptional activity of stress-related genes. nevertheless, recent findings have suggested new layers of regulation and complexity. upstream molecular mechanisms are involved in the plant response to abiotic stress. plants gain resistance to abiotic stress by reprogramming metabolism and gene expression. gaba is proposed to be a signaling molecule involved in nitrogen metab ...201323602379
sequential determination of metabolites involved in the biosynthesis of aromatic amino acids after ultrasound-assisted extraction from plants and reverse lc separation.a dual method is proposed for the determination of metabolites involved in the shikimate pathway which are biomarkers of the effects of glyphosate action on plants exposed to this herbicide. extraction of the target metabolites (phenylalanine, tryptophan, tyrosine and shikimic acid) from a wheat model plant was accelerated by ultrasound energy. after centrifugation and micro-filtration, 1 μl of extract was injected into the chromatograph in an isocratic regime for 4 min to determine shikimate by ...201223598041
differential expression of three bor1 genes corresponding to different genomes in response to boron conditions in hexaploid wheat (triticum aestivum l.).boron (b) is an essential micronutrient for plants. efflux-type b transporters, bors, have been identified in arabidopsis thaliana and rice. here we identified bor1 genes encoding b efflux transporters, from the hexaploid genome of wheat (triticum aestivum l.). we cloned three genes closely related to osbor1 and named them tabor1.1, tabor1.2 and tabor1.3. all three tabor1s showed b efflux activities when expressed in tobacco by-2 cells. tabor1-green fluorescent protein (gfp) fusion proteins were ...201323596187
molecular mechanisms of hmw glutenin subunits from 1s(l) genome of aegilops longissima positively affecting wheat breadmaking quality.a wheat cultivar "chinese spring" chromosome substitution line cs-1s(l)(1b), in which the 1b chromosome was substituted by 1s(l) from aegilops longissima, was developed and found to possess superior dough and breadmaking quality. the molecular mechanism of its super quality conformation is studied in the aspects of high molecular glutenin genes, protein accumulation patterns, glutenin polymeric proteins, protein bodies, starch granules, and protein disulfide isomerase (pdi) and pdi-like protein ...201323593125
enzyme affinity to cell types in wheat straw (triticum aestivum l.) before and after hydrothermal pretreatment.wheat straw used for bioethanol production varies in enzymatic digestibility according to chemical structure and composition of cell walls and tissues. in this work, the two biologically different wheat straw organs, leaves and stems, are described together with the effects of hydrothermal pretreatment on chemical composition, tissue structure, enzyme adhesion and digestion. to highlight the importance of inherent cell wall characteristics and the diverse effects of mechanical disruption and bio ...201323590820
a phosphate starvation response regulator ta-phr1 is involved in phosphate signalling and increases grain yield in wheat.phosphorus deficiency is a major limiting factor for crop yield worldwide. previous studies revealed that phr1 and it homologues play a key role in regulating the phosphate starvation response in plants. however, the function of phr homologues in common wheat (triticum aestivum) is still not fully understood. the aim of the study was to characterize the function of phr1 genes in regulating phosphate signalling and plant growth in wheat.201323589634
resequencing and comparative genomics of stagonospora nodorum: sectional gene absence and effector discovery.stagonospora nodorum is an important wheat (triticum aestivum) pathogen in many parts of the world, causing major yield losses. it was the first species in the large fungal dothideomycete class to be genome sequenced. the reference genome sequence (sn15) has been instrumental in the discovery of genes encoding necrotrophic effectors that induce disease symptoms in specific host genotypes. here we present the genome sequence of two further s. nodorum strains (sn4 and sn79) that differ in their ef ...201323589517
roles for blue light, jasmonate and nitric oxide in the regulation of dormancy and germination in wheat grain (triticum aestivum l.).abscisic acid (aba) plays a central role in seed dormancy and transcriptional regulation of genes coding for aba biosynthetic and degradation enzymes is responsible for control of aba content. however, little is known about signalling both before and after aba regulation, in particular, how environmental signals are perceived and transduced. we are interested in these processes in cereal grains, particularly in relation to the development of strategies for controlling pre-harvest sprouting in ba ...201323588419
soil moisture dynamics modeling considering multi-layer root zone.the moisture uptake by plant from soil is a key process for plant growth and movement of water in the soil-plant system. a non-linear root water uptake (rwu) model was developed for a multi-layer crop root zone. the model comprised two parts: (1) model formulation and (2) moisture flow prediction. the developed model was tested for its efficiency in predicting moisture depletion in a non-uniform root zone. a field experiment on wheat (triticum aestivum) was conducted in the sub-temperate sub-hum ...201323579833
snping aegilops tauschii genetic diversity and the birthplace of bread wheat. 201323577596
the genetic study utility of a hexaploid wheat dh population with non-recombinant a- and b-genomes.to study the d-genome of the wild wheat relative aegilops tauschii cosson at the hexaploid level, we developed a synthetic doubled-haploid (dh) hexaploid wheat population, syndh3. this population was derived from the spontaneous chromosome doubling of triploid f1 hybrid plants obtained from a cross between triticum turgidum ssp. dicoccon pi377655 and a. tauschii ssp. strangulata as66 × ssp. tauschii as87. syndh3 is a diploidization-hexaploid dh population containing recombinant d chromosomes fro ...201323577301
functional characterisation of wheat pgip genes reveals their involvement in the local response to wounding.polygalacturonase-inhibiting proteins (pgips) are cell wall leucine-rich repeat (lrr) proteins involved in plant defence. the hexaploid wheat (triticum aestivum, genome aabbdd) genome contains one pgip gene per genome. tapgip1 (b genome) and tapgip2 (d genome) are expressed in all tissues, whereas tapgip3 (a genome) is inactive because of a long terminal repeat, copia retrotransposon insertion within the coding region. to verify whether tapgip1 and tapgip2 encode active pgips and are involved in ...201323574379
plant regeneration from mature embryo of commercial indian bread wheat (triticum aestivum l.) cultivars.a simple, efficient, reproducible and comparatively genotype-independent in vitro plant regeneration protocol was developed for ten commercial indian bread wheat cultivars using mature embryos as the explants. three different auxins and five different combinations of growth regulators in a modified murashige and skoog's basal medium were assessed for their effect on callus induction and plant regeneration, respectively, in a high yielding and widely grown cultivar, pbw-343. the optimized conditi ...201223573055
identification of rapd and issr markers for drought tolerance in wheat (triticum aestivum l.).drought tolerance is the essential trait that needs to be incorporated in cereal crops, particularly those grown under the rainfed cultivation. drought tolerance being contributed by several regions of the genome requires identification of these regions, using suitable molecular markers. therefore, present investigation was aimed at analyzing the genetic diversity present among the cultivars of rainfed and the irrigated areas with respect to the drought tolerant trait. in all, 14 rapd and 90 iss ...201223573046
mercury induced phytotoxicity and oxidative stress in wheat (triticum aestivum l.) plants.the phytotoxicity and oxidative damage in response to different concentrations of hg (0.0, 2.5, 5.0, 10 and 25 μm) were evaluated in wheat plants. the root and shoot growth, content of chlorophyll and total soluble protein declined at 10 and 25 μm hg. roots of the plant were more affected as compared to the shoot. the malondialdehyde (mda) quantity enhanced in the roots of wheat plants treated with 10 and 25 μm hg and in the leaves of plants treated with 25 μm hg. the concentration of h2o2 decre ...201223573037
absorption efficiency of n, p, k through triple inoculation of wheat (triticum aestivum l.) by azospirillum brasilense, streptomyces sp., glomus intraradices and manure application.this study examined the effects of different biofertilizers and manure on the absorption and absorption efficiency of macronutrients by wheat in alborz province, iran. the experimental design was factorial in the form of a randomized complete block design with three replications. the four factors of the experiment were azospirillum (with 600 g/ha and without), mycorrhiza (with 1 kg/ha and without), streptomyces (with 0.5 kg/ha and without) and manure (with 30 t/ha and without). to measure n, p a ...201123573008
individual and combined effects of waterlogging and alkalinity on yield of wheat (triticum aestivum l.) imposed at three critical stages.response of wheat genotype hd 2329 to individual and combined effects of alkalinity and waterlogging (wl) at tillering, panicle emergence and anthesis stage was studied. both stresses increased na accumulation and reduced k uptake which leads to higher na(+)/k(+) ratio in the leaves. yield was decreased under all the stress treatments and highly correlated with na(+)/k(+) ratio at all the three growth stages (r = -0.83, -0.82 and -0.73, respectively) with maximum reduction under ph 9.4 + wl. inc ...201023572981
effect of water stress on carbon isotope discrimination and rubisco activity in bread and durum wheat genotypes.carbon isotope discrimination (cid) has been suggested as an indirect tool for selection of water use efficiency in wheat. this study evaluates the role of rubisco activity in regulating carbon isotope discrimination (cid) and discriminate the response of durum (pdw-233) and bread (c-306, hd-2329) wheat genotypes for carbon isotope discrimination and rubisco activity under induced water stress hydroponically created by using different concentrations of peg. in c-306, a well known drought toleran ...200923572938
study on the response of diploid, tetraploid and hexaploid species of wheat to the elevated co2.study was done to compare the response of triticum aestivum (hexaploid), triticum durum (tetraploid) and triticum monococcum (diploid) wheat species to the elevated co2 using free air co2 enrichment (face) facility. it was demonstrated that the modern cultivar of wheat triticum aestivum (hexaploid) was largely sink limited. it appeared to have less photosynthesis per unit leaf area than triticum monococcum (diploid wheat). while leaf size, grain weight and amylase activity increased with the plo ...200923572925
effect of germination on the phytase activity, phytate and total phosphorus contents of rice (oryza sativa), maize (zea mays), millet (panicum miliaceum), sorghum (sorghum bicolor) and wheat (triticum aestivum).the effect of germination on the level of phytase activity and the contents of phytates and phosphorus of five nigeria grown cereal grains was studied. the cereals screened were rice (oryza sativa), maize (zea mays), millet (panicum miliaceum), sorghum (sorghum bicolor) and wheat (triticum aestivum). phytase activity was high (0.21-0.67 u g(-1)) in all samples. phytate content ranged between 5.6 and 6.2 mg g(-1) while total phosphorus content ranged between 3.3 and 4.3 mg g(-1). during germinati ...023572811
physiological and biochemical mechanisms of allelopathy mediated by the allelochemical extracts of phytolacca latbenia (moq.) h. walter.in allelopathy, one plant suppresses the growth and development of other plant/plants by negatively affecting a variety of physiological and biochemical reactions. we checked the effects of methanolic extracts (allelochemical extracts) of phytolacca latbenia (moq.) h. walter on antioxidant enzyme activities such as peroxidases (pods), super oxide dismutases (sods) and catalase (cat) and on total protein contents (tpc), cellular injury (ci), and malondialdehyde (mda) in the germinating seeds of b ...201523572390
protective responses induced by herbicide safeners in wheat.safeners are agrochemicals which enhance tolerance to herbicides in cereals including wheat (triticum aestivum l.) by elevating the expression of xenobiotic detoxifying enzymes, such as glutathione transferases (gsts). when wheat plants were spray-treated with three safener chemistries, namely cloquintocet mexyl, mefenpyr diethyl and fenchlorazole ethyl, an apparently identical subset of gsts derived from the tau, phi and lambda classes accumulated in the foliage. treatment with the closely rela ...023564986
identification of qtls associated with seedling root traits and their correlation with plant height in wheat.genetic relationships between plant height and root morphology were investigated in a diverse set of wheat germplasm [199 double-haploid progeny derived from a cross between avalon and cadenza (triticum aestivum l.), rht near-isogenic lines (nils), and accessions from the watkins collection] to investigate whether rht genes controlling shoot height also control seedling root growth. a germination paper screen was developed to measure seedling root length (distinguishing seminal axes from seminal ...201323564959
the effect of two differentially expressed wheat vrn-b1 alleles on the heading time is associated with structural variation in the first intron.we measured the level of vrn-b1 transcripts within near-isogenic lines of triticum aestivum cultivar 'bezostaya 1' carrying two vrn-b1 alleles from cultivars 'saratovskaya 29' and 'diamant 2' (vrn-b1c and vrn-b1a, respectively). both lines have similar kinetics of vrn-b1 transcript accumulation at the third to fifth leaf stages. however, quantitative pcr analysis at the third leaf stage showed that the vrn-b1c allele was transcribed 10 times faster than the vrn-b1a allele. the f1 hybrid between ...201323564445
proteomic analysis on the leaves of tabtf3 gene virus-induced silenced wheat plants may reveal its regulatory mechanism.basic transcription factor 3 (btf3) is involved in the transcriptional initiation of rna polymerase ii and is also associated with apoptosis. in this study, virus-induced gene silencing of tabtf3 caused severe viral symptoms in wheat seedlings, which then displayed stunted growth, reduced height, and decreased total fresh and dry weights. a proteomic approach was further used to identify the protein species showing differential abundance between the tabtf3 virus-induced gene silenced wheat plant ...201323563083
the role of thioredoxin h in protein metabolism during wheat (triticum aestivum l.) seed germination.thioredoxin h can regulate the redox environment in the cell and play an important role in the germination of cereals. in the present study, the thioredoxin s antisense transgenic wheat with down-regulation of thioredoxin h was used to study the role of thioredoxin h in protein metabolism during germination of wheat seeds, and to explore the mechanism of the thioredoxin s antisense transgenic wheat seeds having high resistance to pre-harvest sprouting. the qrt-pcr results showed that the express ...023562797
strategy for exploiting exotic germplasm using genetic, morphological, and environmental diversity: the aegilops tauschii coss. example.hexaploid bread wheat evolved from a rare hybridisation, which resulted in a loss of genetic diversity in the wheat d-genome with respect to the ancestral donor, aegilops tauschii. novel genetic variation can be introduced into modern wheat by recreating the above hybridisation; however, the information associated with the ae. tauschii accessions in germplasm collections is limited, making rational selection of accessions into a re-synthesis programme difficult. we describe methodologies to iden ...201323558983
integration of mate pair sequences to improve shotgun assemblies of flow-sorted chromosome arms of hexaploid wheat.the assembly of the bread wheat genome sequence is challenging due to allohexaploidy and extreme repeat content (>80%). isolation of single chromosome arms by flow sorting can be used to overcome the polyploidy problem, but the repeat content cause extreme assembly fragmentation even at a single chromosome level. long jump paired sequencing data (mate pairs) can help reduce assembly fragmentation by joining multiple contigs into single scaffolds. the aim of this work was to assess how mate pair ...201323557231
haplotype dictionary for the rht-1 loci in wheat.the introduction of reduced height (rht)-b1b and rht-d1b into bread wheat (triticum aestivum) varieties was a key component of the 'green revolution' and today these alleles are the primary sources of semi-dwarfism in wheat. the rht-1 loci encode della proteins, which are transcription factors that affect plant growth and stress tolerance. in bread wheat, rht-d1b and rht-b1b influence resistance to the disease fusarium head blight. to identify rht-1 variants, locus specific primers were develope ...201323553443
global diversity and distribution of three necrotrophic effectors in phaeosphaeria nodorum and related species.population genetic and phylogenetic studies have shown that phaeosphaeria nodorum is a member of a species complex that probably shares its center of origin with wheat (triticum aestivum and triticum durum). we examined the evolutionary histories of three known necrotrophic effectors (nes) produced by p. nodorum and compared them with neutral loci. we screened over 1000 individuals for the presence/absence of each effector and assigned each individual to a multi-effector genotype. diversity at e ...201323550706
transgenic wheat expressing thinopyrum intermedium myb transcription factor timyb2r-1 shows enhanced resistance to the take-all disease.the disease take-all, caused by the fungus gaeumannomyces graminis, is one of the most destructive root diseases of wheat worldwide. breeding resistant cultivars is an effective way to protect wheat from take-all. however, little progress has been made in improving the disease resistance level in commercial wheat cultivars. myb transcription factors play important roles in plant responses to environmental stresses. in this study, an r2r3-myb gene in thinopyrum intermedium, timyb2r-1, was cloned ...201323547108
metabolic indicators of drought stress tolerance in wheat: glutamine synthetase isoenzymes and rubisco.drought stress has a considerable impact on the ecosystem and agriculture. continuous water deficit induces early leaf senescence in plants. during this process, chloroplasts are degraded and photosynthesis drastically drops. the objective of this investigation was to look into the regulation of nitrogen and carbon metabolism during water deficit. rubisco (ribulose-1,5-bisphosphate carboxylase/oxygenase; ec 4.1.1.39) and the total protein contents inform us of the sink-source relation in plants. ...201323542183
fate of cuo and zno nano- and microparticles in the plant environment.the environmental fate of metal oxide particles as a function of size was assessed by comparing the behavior of cuo or zno nanoparticles (nps) to that of the corresponding microparticles (mps) in a sand matrix, with and without wheat (triticum aestivum l.) growth. after 14 days of incubation in the planted sand, the cuo and zno nps were increased from their nominal sizes of <50 nm and <100 nm, to ~317 nm and ~483 nm, respectively. accordingly, the negative surface charge of colloids present in a ...201323540424
novel insights into the composition, variation, organization, and expression of the low-molecular-weight glutenin subunit gene family in common wheat.low-molecular-weight glutenin subunits (lmw-gs), encoded by a complex multigene family, play an important role in the processing quality of wheat flour. although members of this gene family have been identified in several wheat varieties, the allelic variation and composition of lmw-gs genes in common wheat are not well understood. in the present study, using the lmw-gs gene molecular marker system and the full-length gene cloning method, a comprehensive molecular analysis of lmw-gs genes was co ...201323536608
draft genome of the wheat a-genome progenitor triticum urartu.bread wheat (triticum aestivum, aabbdd) is one of the most widely cultivated and consumed food crops in the world. however, the complex polyploid nature of its genome makes genetic and functional analyses extremely challenging. the a genome, as a basic genome of bread wheat and other polyploid wheats, for example, t. turgidum (aabb), t. timopheevii (aagg) and t. zhukovskyi (aagga(m)a(m)), is central to wheat evolution, domestication and genetic improvement. the progenitor species of the a genome ...201323535596
aegilops tauschii draft genome sequence reveals a gene repertoire for wheat adaptation.about 8,000 years ago in the fertile crescent, a spontaneous hybridization of the wild diploid grass aegilops tauschii (2n = 14; dd) with the cultivated tetraploid wheat triticum turgidum (2n = 4x = 28; aabb) resulted in hexaploid wheat (t. aestivum; 2n = 6x = 42; aabbdd). wheat has since become a primary staple crop worldwide as a result of its enhanced adaptability to a wide range of climates and improved grain quality for the production of baker's flour. here we describe sequencing the ae. ta ...201323535592
enhanced resistance to stripe rust disease in transgenic wheat expressing the rice chitinase gene rc24.stripe rust is a devastating fungal disease of wheat worldwide which is primarily caused by puccinia striiformis f. sp tritici. transgenic wheat (triticum aestivum l.) expressing rice class chitinase gene rc24 were developed by particle bombardment of immature embryos and tested for resistance to puccinia striiformis f.sp tritici. under greenhouse and field conditions. putative transformants were selected on kanamycin-containing media. polymease chain reaction indicated that rc24 was transferred ...201323529204
taabc1, a member of abc1-like family involved in hypersensitive response against the stripe rust fungal pathogen in wheat.to search for genes involved in wheat (triticum aestivum l.) defense response to the infection of stripe rust pathogen puccinia striiformis f. sp. tritici (pst), we identified and cloned a new wheat gene similar to the genes in the abc1-like gene family. the new gene, designated as taabc1, encodes a 717-amino acid, 80.35 kd protein. the taabc1 protein contains two conserved domains shared by abc1-like proteins, two trans-membrane domains at the c-terminal, and a 36-amino acid chloroplast targeti ...201323527058
molecular characterisation of the amino- and carboxyl-domains in different glu-a1x alleles of triticum urartu thum. ex gandil.the wild diploid wheat (triticum urartu thum. ex gandil.) is a potential gene source for wheat breeding, as this species has been identified as the a-genome donor in polyploid wheats. one important wheat breeding trait is bread-making quality, which is associated in bread wheat (t. aestivum ssp. aestivum l. em. thell.) with the high-molecular-weight glutenin subunits. in t. urartu, these proteins are encoded by the glu-a1x and glu-a1ay genes at the glu-a (u) 1 locus. the glu-a1x genes of 12 glu- ...201323525634
qtl for root angle and number in a population developed from bread wheats (triticum aestivum) with contrasting adaptation to water-limited environments.root architecture traits in wheat are important in deep soil moisture acquisition and may be used to improve adaptation to water-limited environments. the genetic architecture of two root traits, seminal root angle and seminal root number, were investigated using a doubled haploid population derived from serim82 and hartog. multiple novel quantitative trait loci (qtl) were identified, each one having a modest effect. for seminal root angle, four qtl (-log10(p) >3) were identified on 2a, 3d, 6a a ...201323525632
structural and biochemical characterization of the cytosolic wheat cyclophilin tacypa-1.cyclophilins belong to a family of proteins that bind to the immunosuppressive drug cyclosporin a (csa). several members of this protein family catalyze the cis-trans isomerization of peptide bonds preceding prolyl residues. the present study describes the biochemical and structural characteristics of a cytosolic cyclophilin (tacypa-1) cloned from wheat (triticum aestivum l.). purified tacypa-1 expressed in escherichia coli showed peptidyl-prolyl cis-trans isomerase activity, which was inhibited ...201323519664
alveograph and mixolab parameters associated with puroindoline-d1 genes in chinese winter wheats.grain texture is one of the most important characteristics of bread wheat and has a significant influence on end-use qualities.201323519461
comprehensive analysis of the transcription of starch synthesis genes and the transcription factor rsr1 in wheat (triticum aestivum) endosperm.the cdna sequences of 26 starch synthesis genes were identified in common wheat (triticum aestivum l.), and their transcript levels were measured using quantitative real-time rt-pcr to assess the function of individual genes and the regulatory mechanism in wheat endosperm. the expression patterns of 26 genes in wheat endosperm were classified into three groups. the genes in group 1 were richly expressed in the early stage of grain development and may be involved in the construction of fundamenta ...201223517321
[specific features of fertility restoration in alloplasmic lines obtained based on hybridization of self-fertilized offspring of barley-wheat (hordeum vulgare l. x triticum aestivum l.) amphiploid with common wheat varieties saratovskaya 29 and pyrotrix 28].the problems of fertility restoration in the progeny of barley-wheat hybrids (h. vulgare x t. aestivum) are explained by incompatibility between the cytoplasm of cultivated barley and the nuclear genome of common wheat. suitable models for studying these problems are alloplasmic lines that combine the cytoplasm of barley and the nuclear genome of wheat. in this work, the specific features of fertility restoration in alloplasmic common wheat lines (h. vulgare)-t. aestivum were studied depending o ...201223516898
in silico comparative analysis and expression profile of antioxidant proteins in plants.the antioxidant system in plants is a very important defensive mechanism to overcome stress conditions. we examined the expression profile of antioxidant enzymes superoxide dismutase (sod), catalase (cat) and ascorbate peroxidase (apx) using a bioinformatics approach. we explored secondary structure prediction and made detailed studies of signature pattern of antioxidant proteins in four plant species (triticum aestivum, arabidopsis thaliana, oryza sativa, and brassica juncea). fingerprinting an ...201323512671
diversification of the duplicated f3h genes in triticeae.the f3h gene encodes the flavonoid synthesis enzyme flavanone 3-hydroxylase. unlike most plant genomes, the bread wheat (triticum aestivum l.) b genome has two, rather than just one f3h copy. the paralogous f3h-b2 sequence was isolated by pcr and shown to be transcribed, but its predicted polypeptide differed from the typical f3h sequence at a number of the conserved residues associated with its putative substrate-binding sites. the f3h-b2 promoter region was highly divergent from that of f3h-b1 ...201323503816
effects of feeding algal meal high in docosahexaenoic acid on feed intake, milk production, and methane emissions in dairy cows.this study examined effects on milk yield and composition, milk fatty acid concentrations and methane (ch4) emissions when dairy cows were offered diets containing different amounts of algal meal. the algal meal contained 20% docosahexaenoic acid (dha) and cows were offered either 0, 125, 250, or 375 g/cow per d of algal meal corresponding to 0, 25, 50, or 75 g of dha/cow per d. thirty-two holstein cows in mid lactation were allocated to 4 treatment groups, and cows in all groups were individual ...201323498011
structural characterization of flavonoid glycosides from leaves of wheat (triticum aestivum l.) using lc/ms/ms profiling of the target compounds.the aim of this study was to present integrated mass spectrometric methods for the structural characterization and identification of flavonoid glycoconjugates. during the liquid chromatography/mass spectrometry analyses, triversa nanomate chip-based system with nanoelectrospray ionization and fraction collection was combined to a quadrupole time-of-flight mass spectrometer. in the extract samples prepared from green leaves of wheat plantlets, 41 flavonoid derivatives were recognized. part of the ...201323494788
can root electrical capacitance be used to predict root mass in soil?electrical capacitance, measured between an electrode inserted at the base of a plant and an electrode in the rooting substrate, is often linearly correlated with root mass. electrical capacitance has often been used as an assay for root mass, and is conventionally interpreted using an electrical model in which roots behave as cylindrical capacitors wired in parallel. recent experiments in hydroponics show that this interpretation is incorrect and a new model has been proposed. here, the new mod ...201323493014
molecular cytological characterization of two novel durum--thinopyrum intermedium partial amphiploids with resistance to leaf rust, stem rust and fusarium head blight.thinopyrum intermedium, a wild relative of wheat, is an excellent source of disease resistance. two novel partial amphiploids, 08-47-50 and 08-53-55 (2n = 6x = 42), were developed from wide crosses between durum wheat and th. intermedium. meiotic analysis showed that pollen mother cells of the two partial amphiploids formed an average 20.49 bivalents for 08-47-50 and 20.67 bivalents for 08-53-55, indicating that they are basically cytologically stable. gish analysis revealed that the two partial ...201323489247
uptake and translocation of ti from nanoparticles in crops and wetland plants.bioavailability of engineered metal nanoparticles affects uptake in plants, impacts on ecosystems, and phytoremediation. we studied uptake and translocation of ti in plants when the main source of this metal was tio2 nanoparticles. two crops (phaseolus vulgaris (bean) and triticum aestivum (wheat)), a wetland species (rumex crispus, curly dock), and the floating aquatic plant (elodea canadensis, canadian waterweed), were grown in nutrient solutions with tio2 nanoparticles (0, 6, 18 mmol ti l(-1) ...201323487992
in silico studies of c 3 metabolic pathway proteins of wheat (triticum aestivum).photosynthesis is essential for plant productivity and critical for plant growth. more than 90% of plants have a c3 metabolic pathway primarily for carbon assimilation. improving crop yields for food and fuel is a major challenge for plant biology. to enhance the production of wheat there is need to adopt the strategies that can create the change in plants at the molecular level. during the study we have employed computational bioinformatics and interactomics analysis of c3 metabolic pathway pro ...201223484105
identification of the isoamylase 3 gene in common wheat and its expression profile during the grain-filling period.in higher plants, isoamylase-type starch debranching enzyme catalyzes the α-1,6-glucosidic linkages of glycogen and phytoglycogen. we cloned an isoamylase-type starch debranching enzyme isa3 cdna sequence (2883 bp), designated as taisa3, from common wheat (triticum aestivum), using the rapid amplification of cdna ends method. the open reading frame of taisa3 was found to have 2331 bp, and its deduced amino acid sequence was found to share high similarity with those of other gramineous plant isa3 ...201323479162
a radiation hybrid map of chromosome 1d reveals synteny conservation at a wheat speciation locus.the species cytoplasm specific (scs) genes affect nuclear-cytoplasmic interactions in interspecific hybrids. a radiation hybrid (rh) mapping population of 188 individuals was employed to refine the location of the scs (ae) locus on triticum aestivum chromosome 1d. "wheat zapper," a comparative genomics tool, was used to predict synteny between wheat chromosome 1d, oryza sativa, brachypodium distachyon, and sorghum bicolor. a total of 57 markers were developed based on synteny or literature and g ...201323479086
proteogenomic characterization of novel x-type high molecular weight glutenin subunit 1ax1.1.analysis of portuguese wheat (triticum aestivum l.) landrace 'barbela' revealed the existence of a new x-type high molecular weight-glutenin subunit (hmw-gs) encoded at the glu-a1 locus, which we named 1ax1.1. using one-dimensional and two-dimensional electrophoresis and mass spectrometry, we compared subunit 1ax1.1 with other subunits encoded at the glu-a1 locus. subunit 1ax1.1 has a theoretical molecular weight of 93,648 da (or 91,508 da for the mature protein) and an isoelectric point (pi) of ...201323478438
systems genetics of environmental response in the mature wheat embryo.quantitative phenotypic traits are influenced by genetic and environmental variables as well as the interaction between the two. underlying genetic × environment interaction is the influence that the surrounding environment exerts on gene expression. perturbation of gene expression by environmental factors manifests itself in alterations to gene co-expression networks and ultimately in phenotypic plasticity. comparative gene co-expression networks have been used to uncover biological mechanisms ...201323475987
translocation and degradation of tebuconazole and prothioconazole in wheat following fungicide treatment at flowering.prothioconazole and tebuconazole are among the most effective fungicides against fusarium head blight (fhb) of wheat (triticum aestivum l.). the translocation between the ears and the flag leaves and the kinetics of degradation may influence field efficacy of these active ingredients (ais).201323471588
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