Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| structural analysis of the specific capsular polysaccharide of rhodococcus equi serotype 2. | the specific capsular polysaccharide produced by rhodococcus equi serotype 2 is a high-molecular-weight acidic polymer composed of d-glucose, d-mannose, d-glucuronic acid and 3-o-[(s)-1-carboxyethyl]-l-rhamnose in equimolar proportions. structural analysis, employing a combination of chemical and n.m.r. techniques, established that the polysaccharide is composed of linear repeating tetrasaccharide units. (formula; see text) in which the beta-d-mannose residues carry o-acetyl groups at o-2 and o- ... | 1990 | 2073639 |
| focal pulmonary lesions in patients with aids: percutaneous transthoracic needle biopsy. | the authors performed percutaneous transthoracic needle biopsy (ptnb) in 13 patients with acquired immunodeficiency syndrome (aids) and previously undiagnosed focal pulmonary lesions. findings with ptnb were diagnostic in 11 of 13 cases. complications included minimal hemoptysis in one case and small pneumothoraxes in two cases, one of which required chest tube drainage. the authors did not experience the high complication rate reported previously by some authors who used this diagnostic procedu ... | 1991 | 2068304 |
| cloning of nocardioform dna conferring the ability to inactivate rifampicin. | a novel mechanism of resistance to rifampicin has recently been reported in which this antibiotic is inactivated. here we describe the cloning of dna from a nocardiofrom strain conferring this ability to inactivate rifampicin. cloning was on the basis of complementation, as an increased resistance to the antibiotic. the genetic information was on a 8.3-kb bglii fragment. | 1991 | 2060762 |
| [rhodococcus equi pneumonia in a patient with acquired immunodeficiency syndrome]. | a male patient of 33 years of age with aids developed pneumonia with abscess formation and a slow clinical course. the sputum, bronchoalveolar lavage and blood cultures yielded a gram-positive, partially acid-alcohol resistant bacillus which was identified as rhodococcus equi. this organism has been uncommonly implicated in pneumonia in patients with immunodeficiency, particularly with neoplasia and severe cell immunity deficiency. it has been very rarely reported in patients with aids. to our k ... | 1991 | 2051807 |
| occurrence of two structural types of mercury reductases among gram-positive bacteria. | structural variants of mercury reductase containing the n-terminal domain, which is easily cleaved by trypsin, have been found in gram-positive bacteria with a low genomic g + c content (bacillus, staphylococcus and, possibly, some other genera). mercury reductases without the n-terminal domain and relatively resistant to limited proteolysis are typical for gram-positive bacteria with a high genomic g + c content (arthrobacter, citreobacterium, micrococcus, mycobacterium, rhodococcus). both type ... | 1991 | 2040434 |
| identification of 15- to 17-kilodalton antigens associated with virulent rhodococcus equi. | antigens of rhodococcus equi were analyzed by immunoblotting with naturally infected foal sera. immunoblots of whole-cell antigen preparations of clinical isolates of r. equi revealed that major protein bands with molecular masses of 15 to 17 kda were present in all clinical isolates tested and all isolates virulent for mice. in contrast, the 15- to 17-kda antigens were not identified by immunoblotting in atcc 6939, a type strain of r. equi that was avirulent for mice. whole-cell antigens of 102 ... | 1991 | 2037660 |
| role of antibody to extracellular proteins of rhodococcus equi in protection against r. equi pneumonia in foals. | rhodococcus equi produces two exoenzymes (ree), a cholesterol oxidase in large amounts and a phospholipase c, which cause lysis of sheep red blood cells (srbc) sensitized with staphylococcus aureus beta toxin. two immunization studies were done in foals to determine the role of antibody to ree in protection against r. equi pneumonia. in the first study, three foals (mean age 10 days) were vaccinated four times at 2-week intervals with over 1 million units of partially purified exoenzymes (pree). ... | 1991 | 2031302 |
| rhodococcus equi--an easily missed opportunistic pathogen. | over the last 10 years rhodococcus equi has been identified as an important, albeit very rare, opportunistic pathogen. as the number of patients immunocompromised from hiv infection grows, this microorganism will likely become of increasing clinical importance. infection with r. equi is usually insidious, causing progressive pulmonary disease that is typically pleural based at the time of microbiological diagnosis. as in the case we present, the clinical and microbiological diagnoses may be sign ... | 1991 | 2028222 |
| rhodococcus equi infection in the patient with aids: literature review and report of an unusual case. | rhodococcus equi is an aerobic, intracellular, gram-positive rod-coccus that is partially acid fast. the organism is primarily a pathogen in animals and has only rarely been seen in immunocompromised humans. its most common manifestation is a slowly progressive pneumonia that may cavitate. infections are thought to be acquired via respiratory exposure to animals or soil. r. equi infections are difficult to treat, usually requiring prolonged administration of parenteral antibiotics and often nece ... | 1991 | 2017640 |
| rhodococcus equi infection in patients with and without human immunodeficiency virus infection. | rhodococcus equi is an uncommon pathogen in humans that has occasionally been reported to cause infection in individuals with impaired cellular immunity. we summarize 30 previously published reports of human infection with r. equi and describe one additional case in a patient with aids. eleven (35%) of the patients discussed in this report had aids or human immunodeficiency virus (hiv) infection, which is emerging as the leading cause of immunosuppression in cases of r. equi infection. seventy-s ... | 1991 | 2017613 |
| pathologists urge using some horse sense to avoid doghouse when making diagnoses. | 1991 | 2013916 | |
| cloning and characterization of genes responsible for metabolism of nitrile compounds from pseudomonas chlororaphis b23. | the nitrile hydratase (nhase) of pseudomonas chlororaphis b23, which is composed of two subunits, alpha and beta, catalyzes the hydration of nitrile compounds to the corresponding amides. the nhase gene of strain b23 was cloned into escherichia coli by the dna-probing method with the nhase gene of rhodococcus sp. strain n-774 as the hybridization probe. nucleotide sequencing revealed that an amidase showing significant similarity to the amidase of rhodococcus sp. strain n-774 was also coded by t ... | 1991 | 2013568 |
| characterization of a new cobalt-containing nitrile hydratase purified from urea-induced cells of rhodococcus rhodochrous j1. | a new cobalt-containing nitrile hydratase was purified from extracts of urea-induced cells from rhodococcus rhodochrous j1 in seven steps. at the last step, the enzyme was crystallized by adding ammonium sulfate. nitrile hydratase was a 500-530-kda protein composed of two different subunits (alpha subunit 26 kda, beta subunit 29 kda). the enzyme contained approximately 11-12 mol cobalt/mol enzyme. a concentrated solution of highly purified nitrile hydratase exhibited a broad absorption spectrum ... | 1991 | 2013281 |
| a case of rhodococcus equi brain abscess. | we treated a patient with acquired immunodeficiency syndrome for a brain abscess caused by rhodococcus equi, an actinomycete that usually infects the lung in immunosuppressed hosts. rhodococcus equi brain abscess is an extremely rare lesion that has never been reported in a patient with acquired immunodeficiency syndrome. the infection was cured by lengthy therapy with multiple antibiotics after aspiration of the lesion to identify the infective organism and determine its sensitivity to antibiot ... | 1991 | 2008648 |
| rhodococcus equi: an animal and human pathogen. | recent isolations of rhodococcus equi from cavitatory pulmonary disease in patients with aids have aroused interest among medical microbiologists in this unusual organism. earlier isolations from humans had also been in immunosuppressed patients following hemolymphatic tumors or renal transplantation. this organism has been recognized for many years as a cause of a serious pyogranulomatous pneumonia of young foals and is occasionally isolated from granulomatous lesions in several other species, ... | 1991 | 2004346 |
| cloning and characterization of an amidase gene from rhodococcus species n-774 and its expression in escherichia coli. | for investigation of an unknown open reading frame which is present upstream of the nitrile hydratase (nhase) gene from rhodococcus sp. n-774, a longer dna fragment covering the entire gene was cloned in escherichia coli. nucleotide sequencing and detailed subcloning experiments predicted a single open reading frame consisting of 521 amino acid residues of mr 54,671. the amino acid sequence, especially its nh2-terminal portion, showed significant homology with those of indoleacetamide hydrolases ... | 1991 | 2001397 |
| grouping of some clinically relevant gram-positive rods by automated fatty acid analysis. diagnostic implications. | one hundred and ninety-four strains of aerobically growing gram-positive rods of the genera corynebacterium, actinomyces, arcanobacterium, erysipelothrix, listeria, oerskovia, nocardia, rhodococcus, and of unnamed center for disease control (cdc) groups were checked for cellular fatty acid profiles with the microbial identification system (microbial id, newark, del., usa). in order to obtain unified data usable for the clinical laboratory, 24 or 48 h sheep blood agar cultures were used. it was t ... | 1991 | 2001281 |
| rhodococcus infection of the skin with lymphadenitis in a nonimmunocompromised girl. | a 7-year-old girl had a 4 x 3 x 3 cm nodule on the left wrist with axillary lymphadenopathy. acid-fast bacilli were seen on a smear from a biopsy specimen of this granulomatous skin lesion. a rhodococcus species grew on culture. skin infections caused by rhodococcus may be more common than the few prior case reports suggest. | 1991 | 1999541 |
| surgical-wound infection. | 1991 | 1984178 | |
| a cluster of rhodococcus (gordona) bronchialis sternal-wound infections after coronary-artery bypass surgery. | 1991 | 1984175 | |
| degradation of 2-methylaniline in rhodococcus rhodochrous: cloning and expression of two clustered catechol 2,3-dioxygenase genes from strain ctm. | rhodococcus rhodochrous strain ctm degrades 2-methylaniline mainly via the meta-cleavage pathway. conversion of the metabolite 3-methylcatechol was catalysed by an mr 156,000 catechol 2,3-dioxygenase (c23oi) comprising four identical subunits of mr 39,000. the corresponding gene was detected by using an oligonucleotide as a gene probe. this oligonucleotide was synthesized on the basis of a partial amino acid sequence obtained from the purified enzyme from r. rhodochrous. the structural gene of c ... | 1991 | 1955878 |
| degradation of 2-methylaniline and chlorinated isomers of 2-methylaniline by rhodococcus rhodochrous strain ctm. | rhodococcus rhodochrous strain ctm co-metabolized 2-methylaniline and some of its chlorinated isomers in the presence of ethanol as additional carbon source. degradation of 2-methylaniline proceeded via 3-methylcatechol, which was metabolized mainly by meta-cleavage. in the case of 3-chloro-2-methylaniline, however, only a small proportion (about 10%) was subjected to meta-cleavage; the chlorinated meta-cleavage product was accumulated in the culture fluid as a dead-end metabolite. in contrast, ... | 1991 | 1955877 |
| [multifactorial experiment on the combined effect of rifampicin and microbial polysaccharide in experimental plague infection]. | multifactorial analysis was applied to the study of the combined effect of rifampicin and a microbial polysaccharide in experimental plague infection. the effect of the antibiotic and immunomodulator was shown to be synergistic. on the basis of the study results polynomial statistic models of the second order were designed and nomograms or equal level lines were plotted which provided optimization of the combined chemo- and immunotherapy. | 1991 | 1953167 |
| rhodococcus luteus and rhodococcus erythropolis chronic endophthalmitis after lens implantation. | 1991 | 1951602 | |
| [biosensors for the determination of phenol and benzoate on the basis of rhodococcus cells and enzyme extracts]. | an amperometric biosensor for determination of phenol, cresol, benzoate and 2-methyl-4-chlorophenol using rhodococcus-cells and enzyme extracts of rhodococcus has been developed. the influence of cultivation of rhodococcus-cells and preincubation of the biosensor with desired substrate on sensibility and specificity was been investigated. in relation to cultivation and preincubation the rhodococcus sensor was high specific to benzoate or phenol and cresol. a linear range was obtained for phenol ... | 1991 | 1950199 |
| the effect of experimental infection with rhodococcus equi on immunodeficient mice. | to investigate the pathogenesis of respiratory lesions caused by the facultative intracellular pathogen, rhodococcus equi, pulmonary clearance was compared in four groups of genetically defined mice, chosen for their specific deficits in immune and inflammatory responses. complement-deficient a/j, immunodeficient nu/nu (nude), scid/scid.bg/bg (scid/beige), c57bi/6j.bg/bg (beige) and normal swiss mice (sw) received approximately 10(7) r. equi intranasally on day 0. bacterial clearance was assesse ... | 1991 | 1949550 |
| purification, cloning, and primary structure of a new enantiomer-selective amidase from a rhodococcus strain: structural evidence for a conserved genetic coupling with nitrile hydratase. | a new enantiomer-selective amidase active on several 2-aryl propionamides was identified and purified from a newly isolated rhodococcus strain. the characterized amidase is an apparent homodimer, each molecule of which has an mr of 48,554; it has a specific activity of 16.5 mumol of s(+)-2-phenylpropionic acid formed per min per mg of enzyme from the racemic amide under our conditions. an oligonucleotide probe was deduced from limited peptide information and was used to clone the corresponding g ... | 1991 | 1938876 |
| association between a large plasmid and 15- to 17-kilodalton antigens in virulent rhodococcus equi. | rhodococcus equi strains showing 15- to 17-kda antigens in immunoblots were found to be virulent in mice. to study the genes specific to these antigens in virulent r. equi, we compared plasmid profiles, immunoblot profiles, and murine pathogenicity profiles of 10 strains of r. equi. all the strains showing 15- to 17-kda antigens contained a large plasmid of approximately 85 kbp and were virulent in mice; however, the remaining strains lacked both the antigens and the large plasmid and were aviru ... | 1991 | 1937765 |
| polyphasic taxonomic study of the genera gordona and tsukamurella including the description of tsukamurella wratislaviensis sp. nov. | representatives of the genera gordona and tsukamurella together with related actinomycetes were the subject of chemotaxonomic, numerical taxonomic and dna homology studies. in the numerical analysis the organisms were examined for 116 unit characters and the data sorted using the simple matching, jaccard and pattern coefficients; clustering was achieved using the unweighted pair group method with arithmetic averages algorithm. the numerical groupings were not affected by the statistics used or b ... | 1991 | 1930572 |
| rhodococcus equi causing human pulmonary infection: review of 29 cases. | rhodococcus equi is a gram-positive pleomorphic bacillus that has been identified as a life-threatening pulmonary pathogen in the immunocompromised host. infection with r equi may go unrecognized by physicians unacquainted with its presentation and unaware of the organism's ability to mimic diphtheroids and to stain weakly positive with an acid-fast stain. | 1991 | 1925723 |
| [ultrastructural features of rhodococcus rubropertinctus and streptococcus lactis dissociants]. | three rhodococcus rubropertinctus dissociants (r, s and m) and two streptococcus lactis dissociants (r and s) were compared using the electron microscopy method of negative contrasting. the cell wall of r.rubropertinctus dissociants had a thickness of 40 nm (r), 30 nm (s) and 20 nm (m). the cell wall of s. lactis dissociants was 35-55 nm (r) and 25-30 nm (s) thick. 1.5-2-fold variations in the thickness of cell walls could account for the different resistance of the dissociants against the actio ... | 1991 | 1921769 |
| characterization of rrh4273i, a restriction-modification system of rhodococcus rhodochrous atcc 4273 (nocardia corallina) which recognizes the same sequence as the streptomyces albus g sali restriction-modification system. | rhodococcus rhodochrous atcc 4275 (nocardia corallina) has a restriction-modification system with the same recognition sequence, methylation site and cleavage site as the sali restriction-modification system. both the restriction endonuclease and the dna-methyltransferase (dna-mtase) have been partially purified and characterized. the nuclease has requirements of activity similar to sali, and a native mr of about 46,000. the dna-mtase is a protein with an mr of about 67,000. no dna homology was ... | 1991 | 1919505 |
| rapid confirmation of listeria monocytogenes isolated from foods by a colony blot assay using a digoxigenin-labeled synthetic oligonucleotide probe. | an oligodeoxyribonucleotide probe based on the sequence of a 321-bp internal fragment of the msp gene encoding a major secreted polypeptide of listeria monocytogenes was labeled with digoxigenin by using terminal deoxynucleotidyl transferase. the specificity of the digoxigenin-labeled probe was determined by dot blot assays. the probe reacted with all strains of l. monocytogenes tested (12 of 12 strains representing five serotypes). the probe did not react with any other listeria species or with ... | 1991 | 1908203 |
| microbial lipids and stable foam formation in the activated sludge process. | the presence of fats and oils in sewage has been related to the formation of stable foams in activated sludge treatment systems. foam forming microbes can utilise and, in some cases, store lipid substrates. since surface lipids would confer the hydrophobicity necessary for flotation on the sludge biomass, the extractable lipids in foaming and non-foaming biomass samples were examined. both pure mono-cultures and sludge samples were used. the results showed that, whilst there were some difference ... | 1991 | 1907713 |
| infections with rhodococcus equi in children. | three cases of serious infection in children, including the first two reports of bacteremia, due to rhodococcus equi are described. only seven pediatric cases have been reported to date. in the laboratory, r. equi can easily be misidentified as a nonpathogenic corynebacterium spp. (diphtheroid) or a mycobacterium spp. despite an overall mortality rate of 25% in adults, no pediatric deaths have occurred due to r. equi. the organism is generally susceptible to vancomycin, aminoglycosides, chloramp ... | 1991 | 1889184 |
| accumulation of a poly(hydroxyalkanoate) copolymer containing primarily 3-hydroxyvalerate from simple carbohydrate substrates by rhodococcus sp. ncimb 40126. | a number of taxonomically-related bacteria have been identified which accumulate poly(hydroxyalkanoate) (pha) copolymers containing primarily 3-hydroxyvalerate (3hv) monomer units from a range of unrelated single carbon sources. one of these, rhodococcus sp. ncimb 40126, was further investigated and shown to produce a copolymer containing 75 mol% 3hv and 25 mol% 3-hydroxybutyrate (3hb) from glucose as sole carbon source. polyesters containing both 3hv and 3hb monomer units, together with 4-hydro ... | 1991 | 1888716 |
| typing of rhodococcus equi isolated from submaxillary lymph nodes of pigs in japan. | isolation of rhodococcus equi from the submaxillary lymph nodes of pigs, with or without caseous lymphadenitis, and typing of the isolates by two serological methods were carried out. the rate of isolation of the organisms from the lymph nodes of pigs was 5 times higher in the lymph nodes with caseous lymphadenitis than in those without the lesion. of 219 isolates, 146 (66.7%) were typable by the method of prescott, while all the 219 isolates (100%) were typable by the method of nakazawa et al. ... | 1991 | 1887702 |
| mycolic acid-containing glycolipid as a possible virulence factor of rhodococcus equi for mice. | by the use of various rhodococcus equi strains differing in the length of carbon chains of glycolipid, we examined whether the glycolipid, glucose monomycolate, was contributing to the virulence of r. equi for mice. r. equi strains with longer carbon chain mycolic acid showed a higher virulence as determined by lethality and granuloma formation in mice than those with shorter ones. when purified glycolipid was injected into mice, granuloma formation and liver damage were most prominent with the ... | 1991 | 1870435 |
| [rhodococcus erythropolis infection in hiv-associated immunodeficiency]. | we report the first case in the literature of human infection with rhodococcus erythropolis in an hiv-positive patient. a 24-year-old bisexual flight attendant had severe hiv-associated immunodeficiency with a cd4 cell count of 0.02 g/l. he complained of multiple subcutaneous nodules at different sites on the extremities. biopsy of one node at his left knee revealed granulomatous inflammation filled with acid-fast rods. these bacteria were identified as rhodococcus erythropolis. the disseminated ... | 1991 | 1866607 |
| relapsing systemic infection due to rhodococcus equi in a drug abuser seropositive for human immunodeficiency virus. | a case of recurrent multisystemic infection due to rhodococcus equi in a patient with antibody to the human immunodeficiency virus (hiv) but without aids is reported. this case, which occurred in a woman with a history of alcohol and drug abuse, is, to our knowledge, the first such case described. the infection involved the lungs, kidneys, brain, and bloodstream. despite several courses of therapy with antibiotics to which her isolates of r. equi were susceptible in vitro, the patient experience ... | 1991 | 1866557 |
| lung abscess caused by rhodococcus equi. | in the immunocompromised patient, early diagnosis of a lung cavity is essential for appropriate treatment. rhodococcus equi (formerly corynebacterium equi) is a variably acid-fast bacterium that can produce cavitary disease in an immunocompromised host. the two cases presented here demonstrate the clinical and radiographic features of r equi lung abscess. the first patient was on long-term corticosteroid therapy for rheumatoid arthritis. the second patient had aids. the correct diagnosis in both ... | 1991 | 1856905 |
| crystallization of a photosensitive nitrile hydratase from rhodococcus sp. n-771. | a photosensitive nitrile hydratase from rhodococcus sp. n-771 has been crystallized in two different crystal forms in its inactive form. one crystal form belongs to an orthorhombic space group p2(1)2(1)2 with unit cell dimensions of a = 117.4 a, b = 145.7 a and c = 52.1 a, and the other form belongs to a hexagonal space group p6(3)22 with unit cell dimensions of a = 110.2 a and c = 412.1 a. | 1991 | 1856857 |
| activator proteins for glycosphingolipid hydrolysis by endoglycoceramidases. elucidation of biological functions of cell-surface glycosphingolipids in situ by endoglycoceramidases made possible using these activator proteins. | endoglycoceramidase (egcase) cleaves the linkage between oligosaccharides and ceramides of various glycosphingolipids (ito, m., and yamagata, t. (1986) j. biol. chem. 261, 14278-14282). recently, by extensive purification, it was separated from cell-lytic factor (hemolysin) and found to consist of three molecular species each with its own specificity (egcases i, ii, and iii) (ito, m., and yamagata, t. (1989) j. biol. chem. 264, 9510-9519). a detergent was required for egcases to express full act ... | 1991 | 1850427 |
| cloning, nucleotide sequence and expression in escherichia coli of two cobalt-containing nitrile hydratase genes from rhodococcus rhodochrous j1. | rhodococcus rhodochrous j1 produces two kinds of cobalt-containing nitrile hydratases (nhases); one is a high molecular mass-nhase (h-nhase) and the other is a low molecular mass-nhase (l-nhase). both nhases are composed of two subunits of different sizes (alpha and beta subunits). the h- and l-nhase genes were cloned into escherichia coli by a dna-probing method using the nhase gene of rhodococcus sp. n-774, a ferric ion-containing nhase producing strain, as the hybridization probe and their nu ... | 1991 | 1840499 |
| use of a siderophore detection medium, ethylene glycol degradation, and beta-galactosidase activity in the early presumptive differentiation of nocardia, rhodococcus, streptomyces, and rapidly growing mycobacterium species. | a simplified scheme for the presumptive early identification of nocardia, rhodococcus, rapidly growing mycobacterium, and streptomyces species is presented. the nocardia and streptomyces spp. and the mycobacterium spp. were positive. the spp. were positive for siderophore activity, but only 25% of the rhodococcus spp. were positive. the rhodococcus and mycobacterium spp. were negative for beta-galactosidase, while the nocardia and streptomyces spp. were positive. the nocardia and streptomyces sp ... | 1991 | 1832172 |
| [pneumonia caused by rhodococcus equi in a patient with aids]. | 1991 | 1822165 | |
| [cavitated pneumonia caused by rhodococcus equi in an hiv-positive female patient]. | 1991 | 1822164 | |
| [bacteremic pneumonia caused by rhodococcus equi and hiv infection. report of a new case and review of the literature]. | rhodococcus equi (corynebacterium equi) is able to produce infections not only in animals but also in patients, usually immunosuppressed ones. we report a new case of bacteremic pneumonia in a drug addict who was also infected by hiv. x-ray film of the chest showed a cavitated infiltrate in right upper lobe. r. equi was recovered from blood, respiratory secretions and lung tissue. the patient was with a prolonged course of antibiotics and also surgical treatment. in spite of both therapies, the ... | 1991 | 1822154 |
| catabolism of 1,3-dinitrobenzene by rhodococcus sp. qt-1. | the 1,3-dinitrobenzene-degrading rhodococcus strain qt-1 was isolated under nitrogen limiting conditions from contaminated soil samples. experimental data indicate tha 1,3-dinitrobenzene is metabolized via 4-nitrocatechol. both compounds were oxidized by resting cells and nitro groups were completely eliminated as nitrite. strain qt-1 utilizes both 1,3-dinitrobenzene and 4-nitrocatechol as source of nitrogen in the absence as well as in the presence of high amounts of ammonia. growth on 4-nitroc ... | 1991 | 1814279 |
| severe rhodococcus equi pneumonia: case report and literature review. | rhodococcus equi is an aerobic, gram-positive, non-motile pleomorphic bacillus infecting immunocompromised patients. forty-nine cases of rhodococcus equi infection have been reported, mainly in patients infected with the human immunodeficiency virus (hiv). a case in which rhodococcus equi caused severe pulmonary infection, the most common presentation, is described. clinically, patients have symptoms of pneumonia with hemoptysis as a prominent feature. x-ray will often show a cavitating upper-lo ... | 1991 | 1810733 |
| time course dependent changes in contents and physical properties of glycolipid species in rhodococcus rhodochrous. | the yield of trehalose dimycolate (tdm), the major glycolipid species elaborated by rhodococcus rhodochrous, a producer of approx. c40-mycolic acid, was not constant in cells cultured for different periods of time. from cells collected at 24, 36, 72, 144 and 172 h of cultivation the following percentages of tdm in diethyl ether soluble lipids (desl) were found: 10.8%, 23.4%, 10.0%, 9.0% and 5.0%, respectively. in turn, the cellular content accounted for approx. 0.6%, 1.2%, 0.9%, 0.6% and 0.2%, r ... | 1991 | 1804566 |
| selective medium containing fosfomycin, nalidixic acid, and culture supernatant of rhodococcus equi for isolation of corynebacterium pseudotuberculosis. | fnr medium containing fosfomycin, nalidixic acid, bovine blood and culture supernatant of rhodococcus equi was prepared by the present authors, and the medium did not inhibit growth of corynebacterium pseudotuberculosis but completely hampered the growth of bacillus subtilis, staphylococcus aureus, and escherichia coli. the culture supernatant of r. equi facilitated detection of suspected colonies of c. pseudotuberculosis due to synergistic hemolysis. rate of isolation of the organisms (from the ... | 1991 | 1796687 |
| protection against naturally acquired rhodococcus equi pneumonia in foals by administration of hyperimmune plasma. | a 2-year field study was performed to determine the capability of increasing rhodococcus equi specific antibody in foals via plasma transfusion or mare vaccination, to determine the kinetics of r. equi (elisa) antibody decay and to assess the protective effects of these procedures in foals on a farm endemic for r. equi. plasma donors were vaccinated with a killed r. equi bacterin and produced high levels of anti-r. equi antibodies, which were harvested by plasmapheresis. in experiment 1, 68 foal ... | 1991 | 1795301 |
| purification and properties of cholesterol oxidase and choline phosphohydrolase from rhodococcus equi. | cholesterol oxidase (co) and choline phosphohydrolase (cph) exoenzymes were isolated from culture supernatants of rhodococcus equi atcc 33701 and their hemolytic and cytotoxic activities examined. the purifications involved differential ammonium sulphate precipitation, ion exchange and gel filtration chromatography. a purification of 32.8-fold and a yield of 0.3% of co were determined by synergistic hemolysis of sheep red blood cells (srbc) presensitized with staphylococcus aureus beta toxin. th ... | 1991 | 1790488 |
| microbial metabolism of quinoline and related compounds. xii. isolation and characterization of the quinoline oxidoreductase from rhodococcus spec. b1 compared with the quinoline oxidoreductase from pseudomonas putida 86. | quinoline oxidoreductase from rhodococcus spec. b1 was purified 39-fold to apparent homogeneity in a 5-step procedure with a recovery of 26%. the mr of the native enzyme as determined by gel chromatography was 300,000. sds polyacrylamide gel electrophoresis of the enzyme revealed 3 protein bands corresponding to mr 82,000, 32,000, and 18,000. the enzyme contains 1.3 atoms of molybdenum, 8 atoms of iron, 8 atoms of acid-labile sulphur, 2 molecules of fad and 2 molecules of molybdopterin cytosine ... | 1991 | 1789933 |
| in-vitro susceptibility of rhodococcus equi to 27 antibiotics. | 1991 | 1778862 | |
| conversion of endoglycoceramidase-activator ii by trypsin to the 27.9 kda polypeptide possessing full activity: purification of activator for endoglycoceramidase by trypsin treatment followed by trypsin-inhibitor agarose column application. | endoglycoceramidase (egcase) cleaves the linkage between oligosaccharides and ceramides of various glycosphingolipids [ito, m. & yamagata, t. (1986) j. biol. chem. 261, 14278-14282]. a detergent was required for egcase to express full activity, possibly due to its hydrophobic nature. recently, activator proteins responsible for stimulating egcase activity in the absence of detergents were isolated from the culture supernatant of rhodococcus sp. [ito, m., ikegami, y., & yamagata, t. (1991) j. bio ... | 1991 | 1769958 |
| prevalence of virulent rhodococcus equi in isolates from soil and feces of horses from horse-breeding farms with and without endemic infections. | the prevalence of virulent rhodococcus equi in isolates from soil and feces of foals on a farm with endemic r. equi infections was significantly higher than that of a farm with no history of the disease. foals bred on a farm with the endemic disease might be constantly exposed to virulent r. equi in their environment. | 1991 | 1757567 |
| rhodococcus equi plasmids: isolation and partial characterization. | fifty-four strains of rhodococcus equi from different clinical sources (mainly horses and pigs) were examined for their plasmid content by two screening methods. plasmids were detected in 49 of 54 strains. a plasmid of approximately 80 kb was isolated from 21 of 22 isolates from horses and 20 of 28 isolates from pigs, and a 105-kb plasmid was isolated from 7 of 28 isolates from pigs. the 80-kb plasmid was significantly associated with strains of equine rather than porcine origin, and the 105-kb ... | 1991 | 1757535 |
| electrophoretic heterogeneity of ribosomal protein at-l30 among actinomycete genera. | the ribosomal proteins from 17 type strains of species belonging to various actinomycete genera were compared by two-dimensional polyacrylamide gel electrophoresis. i detected a striking variability among certain ribosomal proteins (designated at-l30 proteins) with respect to electrophoretic mobility in the first dimension. in contrast, such variability was not observed among ribosomal l30 proteins from other bacteria, such as escherichia coli, bacillus subtilis, pseudomonas aeruginosa, and stap ... | 1992 | 1736962 |
| microbial bioconversion of pollutants. | microorganisms totally detoxicate xenobiotics of various chemical structures, which are serious and, in some cases, very hazardous pollutants. at present, the efforts of a number of researchers promoted the establishment in this country of a collection of microorganisms able to degrade volatile toxic pollutants--toluene, isomeric xylenes, styrene, alpha-methylstyrene, crotonaldehyde; widely distributed xenobiotics chlorobenzoic acids; isomeric aryldicarboxylic acids; and ecologically hazardous p ... | 1992 | 1732995 |
| phylogeny of the whipple's-disease-associated bacterium. | efforts to culture and identify the intracellular bacteria associated with whipple's disease have been unsuccessful. nucleotide sequencing and amplification by the polymerase chain reaction was done on the bacterial 16 s ribosomal dna present in a small-bowel biopsy specimen taken from a patient with whipple's disease. a search by computer for similar rrna sequences filed in databases showed the whipple's-associated organism to be most similar to bacteria of the rhodococcus, streptomyces, and ar ... | 1991 | 1714530 |
| induction of interferons (ifns) and tumor necrosis factor (tnf) in mice by a novel glycolipid trehalose 2,3,6'-trimycolate from rhodococcus aurantiacus (gordona aurantiaca). | the immunomodifying activity of a novel mycoloyl glycolipid, trehalose 2,3,6'-trimycolate (gagm), from a unique psychrophilic acid-fast bacterium, rhodococcus aurantiacus, was examined. icr mice were primed intravenously (i.v.) or intraperitoneally (i.p.) with liposomes containing gagm (300 micrograms/mouse), and were administered lps dissolved in saline (25 micrograms/mouse, i.v.) 2 weeks later. two hours after injection of lps, interferons (ifns) and tumor necrosis factor (tnf) were induced si ... | 1990 | 1699114 |
| imipenem/teicoplanin for rhodococcus equi pulmonary infection in aids patients. | 1991 | 1672417 | |
| imipenem/vancomycin for rhodococcus equi pulmonary infection in hiv-positive patient. | 1991 | 1671281 | |
| cavitating pneumonia caused by rhodococcus equi in a patient with aids. | 1991 | 1669798 | |
| illegitimate integration of non-replicative vectors in the genome of rhodococcus fascians upon electrotransformation as an insertional mutagenesis system. | electrotransformation of rhodococcus fascians by non-replicating plasmids containing a suitable resistance marker resulted in stable transformants by integration of these constructs at various sites in the genome, thereby generating different mutations. tagged genes could be isolated in escherichia coli owing to the presence of a coie1 replicon and an ampicillin resistance gene in the inserted sequences. southern analysis and nucleotide sequencing revealed that recombination can occur at defined ... | 1991 | 1662759 |
| microbial metabolism of quinoline and related compounds. x. the molybdopterin cofactors of quinoline oxidoreductases from pseudomonas putida 86 and rhodococcus spec. b1 and of xanthine dehydrogenase from pseudomonas putida 86. | the bis(carboxamidomethyl) derivatives of the molybdenum cofactors in three eubacterial molybdo-iron/sulphur-flavoproteins were examined. the quinoline oxidoreductases from pseudomonas putida 86 and rhodococcus spec. b1 contain molybdopterin cytosine dinucleotide. in xanthine dehydrogenase from pseudomonas putida 86, however, only molybdopterin was found. the bis(carboxamidomethyl) derivatives of all three enzymes were treated with nucleotide pyrophosphatase, but only those of the quinoline oxid ... | 1991 | 1657036 |
| [in vitro activity of twenty antibiotics against rhodococcus equi]. | the in vitro susceptibility of nine rhodococcus equi strains (seven isolates from immunocompromised patients mainly hiv positive and two reference strains) to twenty various antibiotics were assessed for bacteriostatic effects by an agar dilution method. imipenem and ceftriaxone were the most effective of the beta-lactams studied. the lowest mic were noted with vancomycin, teicoplanin, erythromycin, clarithromycin, rifampicin, gentamicin and doxycycline. a longitudinal survey, including three st ... | 1991 | 1652730 |
| development of a host-vector system in a rhodococcus strain and its use for expression of the cloned nitrile hydratase gene cluster. | two different types of plasmid were isolated from strains of rhodococcus rhodochrous. two plasmids, of the same type but from different strains, were combined with escherichia coli plasmids carrying antibiotic resistance markers to develop e. coli-rhodococcus shuttle vectors. the ampicillin and kanamycin resistance markers served for selection in rhodococcus. electroporation was used to introduce recombinant plasmid dna into r. rhodochrous atcc 12674 at a frequency of 5 x 10(7) transformants per ... | 1992 | 1645124 |
| immunohistochemical observations on pneumonic lesions caused by rhodococcus equi in foals. | an immunohistochemical analysis of rhodococcus equi-induced pneumonia in 10 foals was performed by biotin-streptavidin system. the detection of r. equi was more sensitive in immuno-stain using anti-r. equi serum than in gram's stain. this bacteria also reacted to anti-bcg serum. lysozyme and alpha 1-antitrypsin were detectable in macrophages. a particularly intense staining was observed in association with intracellular bacteria. though a degree of reaction for alpha 1-antichymotrypsin was very ... | 1992 | 1643174 |
| [pulmonary infection caused by rhodococcus equi in a renal transplant recipient]. | rhodococcus equi is a primarily pathogen in animals and it has only rarely been seen in immunocompromised humans. we describe our experience with an hiv-negative patient with a functioning renal graft under cyclosporin-azathioprine-prednisone therapy. the patient died after a two months recurrent multifocal pneumonia. the cultures from sputum and blood were negatives but r. equi was isolated from lungs in the necropsy. most of the 41 cases we had found in the literature had aids or hiv infection ... | 1992 | 1635409 |
| an assay to quantitate the binding of rhodococcus equi to macrophages. | a rhodococcus equi radiobinding assay has been developed using organisms labeled with 3h-uracil. these labeled organisms resemble their unlabeled counterparts with respect to colony morphology, viability, and buoyant density. bacteria routinely incorporate between 5 x 10(-3) and 5 x 10(-2) counts per minute per colony forming unit (cfu) which in this assay allows the detection of fewer than 0.2 cfu per macrophage. once incorporated, greater than 90% of the label remains bacterial associated for ... | 1992 | 1632069 |
| a colorimetric assay for measuring cell-free and cell-bound cholesterol oxidase. | cholesterol oxidase (cholesterol:oxygen oxidoreductase, ec 1.1.3.6) catalyzes the conversion of sterol delta 5-3 beta-alcohol to the corresponding delta 4-3-ketone with the reduction of oxygen to hydrogen peroxide. rhodococcus species gk 1, a soil isolated microbe, produces an extracellular and a membrane-bound cholesterol oxidase; the latter is bound to the outer surface of the microbial cell membrane. a simple and sensitive assay is described to measure the two enzyme types; no enzyme extracti ... | 1992 | 1630278 |
| high-performance liquid chromatography of corynomycolic acids as a tool in identification of corynebacterium species and related organisms. | a high-performance liquid chromatography (hplc) study of 307 strains of corynebacterium species and related taxa revealed that strains classified as "corynebacterium aquaticum"; "corynebacterium asperum"; and centers for disease control (cdc) groups 1, 2, a-3, a-4, a-5, b-1, b-3, e, f-2, and i-2 as well as some unidentified coryneforms do not contain any corynomycolic acids; therefore, they should not be included in the genus corynebacterium. such an hplc method of identification permitted the c ... | 1992 | 1624556 |
| naphthalene degradation via salicylate and gentisate by rhodococcus sp. strain b4. | rhodococcus sp. strain b4, isolated from a soil sample contaminated with polycyclic aromatic hydrocarbons, grows with naphthalene as the sole source of carbon and energy. salicylate and gentisate were identified as intermediates in the catabolism of naphthalene. in contrast to the well-studied catabolic pathway encoded by the nah7 plasmid of pseudomonas putida, salicylate does not induce the genes of the naphthalene-degradative pathway in rhodococcus sp. strain b4. the key enzymes of naphthalene ... | 1992 | 1622263 |
| hemolytic interactions of dermatophilus congolensis. | the strains of dermatophilus congolensis grew on blood agar with washed sheep erythrocytes with marked total hemolysis. in testing for hemolytic interactions they gave a significant synergistic effect of a characteristic shape with rhodococcus equi and streptococcus agalactiae, whereas with staphylococcus aureus producing beta hemolysin and with staphylococcus aureus producing delta hemolysin a simultaneous synergistic as well as antagonistic effect were observed. first of all a conspicuous inhi ... | 1992 | 1621476 |
| virulence-associated 15- to 17-kilodalton antigens in rhodococcus equi: temperature-dependent expression and location of the antigens. | virulent rhodococcus equi showing 15- to 17-kda antigens, which is virulent in mice, was found to harbor an 85-kbp plasmid, and the 15- to 17-kda antigens were found to be associated with possession of the 85-kbp plasmid of r. equi (s. takai, t. sekizaki, t. ozawa, t. sugawara, y. watanabe, and s. tsubaki, infect. immun. 59:4056-4060, 1991). the expression of these antigens was temperature regulated: when cells were grown at a low temperature (25 to 32 degrees c), they did not express them, wher ... | 1992 | 1612765 |
| polyacrylamide gel electrophoresis analysis of ribosomal protein: a new approach for actinomycete taxonomy. | the ribosomal (r)-proteins from eleven streptomyces strains representing various numerical taxonomic clusters were compared by two-dimensional polyacrylamide-gel electrophoresis (2d-page). the protein patterns were specific for each species. an attempt was made to identify one strain of streptomyces by both traditional taxonomic methods and 2d-page analysis of the r-protein patterns. both methods identified the strain as streptomyces lavendulae, and protein pattern analysis also showed that s. g ... | 1992 | 1612446 |
| in-vitro antimicrobial susceptibility of rhodococcus equi. | rhodococcus equi is an intracellular facultative, gram-positive cocco-bacillary organism of increasing importance as a pulmonary pathogen in hiv-positive patients. this study was carried out to evaluate the optimal antibiotic combinations for treating such infections. four human r. equi isolates and one reference strain were tested for their susceptibilities to 36 antibiotics. in-vitro the most active antibiotics were amikacin, gentamicin, netilmicin, erythromycin, clarithromycin, roxithromycin, ... | 1992 | 1607327 |
| [rhodococcus equi in hiv infected patients: 2 new cases]. | we reported two hiv infected patients with bacteremia and pneumonia due to rhodococcus equi. none of them had suffer any opportunistic infection before this episode. clinical presentation includes respiratory tract symptoms of subacute onset and fever. the x-ray examination in both cases revealed pneumonia and lung abscess in upper lobes as well as lung infiltrates in other lobes. the microorganism was isolated in lung fine needle aspiration, bronchoalveolar lavage and blood cultures in both cas ... | 1992 | 1606224 |
| nad-linked, factor-dependent formaldehyde dehydrogenase or trimeric, zinc-containing, long-chain alcohol dehydrogenase from amycolatopsis methanolica. | nad-linked, factor-dependent formaldehyde dehydrogenase (fd-fa1dh) of the gram-positive methylotrophic bacterium, amycolatopsis methanolica, was purified to homogeneity. it is a trimeric enzyme with identical subunits (molecular mass 40 kda) containing 6 atoms zn/enzyme molecule. the factor is a heat-stable, low-molecular-mass compound, which showed retention on an aminex hpx-87h column. inactivation of the factor occurred during manipulation, but activity could be restored by incubation with di ... | 1992 | 1597190 |
| case report: rhodococcus equi pneumonia in a patient infected by the human immunodeficiency virus. | rhodococcus equi, a facultative intracellular bacterium, is a common cause of pulmonary infection in farm animals, especially foals. pulmonary and disseminated infection caused by this organism is occasionally seen in humans, especially in patients whose cell-mediated immunity has been altered by glucocorticoids or cytotoxic chemotherapy. not surprisingly, the organism may cause serious disease in human immunodeficiency virus (hiv)-infected humans whose t cell-dependent immune system has been pr ... | 1992 | 1595781 |
| [the sulfate-reducing capacity of bacteria in the genus pseudomonas]. | active sulphate-reducing microorganism which belongs to the genus pseudomonas has been distinguished and described. the culture is a facultative aerobe, optimum eh is -170-180 mv. pseudomonas sp. being cultivated under strictly anaerobic conditions sulphate-reduction proceeds more intensively than under aerobic conditions. this fact should be taken into account under treatment of industrial sewage. | 1992 | 1584088 |
| isolation and characterization of cesium-accumulating bacteria. | cesium-accumulating bacteria, strains cs98 and cs402, were isolated from soil by a radioactive autoradiographic method using 137cs. these strains displayed the rod-coccus growth cycle and contained mesodiaminopimelic acid, mycolic acids, and tuberculostearic acids. the major menaquinone of cs98 was mk-8(h2). on the basis of these characteristics, strain cs98 was identified as rhodococcus erythropolis and strain cs402 was classified in the genus rhodococcus. the maximum values of cesium removal e ... | 1992 | 1575473 |
| rhodococcus equi infection in patients with aids. | rhodococcus equi is an emerging opportunistic pathogen of hiv-i infected patients. it is an aerobic, gram-positive coryneform bacterium which acts as a facultative intracellular micro-organism, multiplying in the phagosome of macrophages. eighteen cases of r. equi infection in hiv-i positive patients have now been reported. sixteen of these had pneumonia, of which 12 had cavitating lung lesions. a history of contact with farm animals, which are the primary hosts of r. equi, was found in only thr ... | 1992 | 1569304 |
| light-induced oxidation of iron atoms in a photosensitive nitrile hydratase. | the photoactivation process of a photosensitive nitrile hydratase (nhase) from rhodococcus sp. n-771 has been investigated by 57fe mössbauer spectroscopy and magnetic susceptibility measurements in order to clarify the behavior of iron atoms in the enzyme. mössbauer spectra of inactive nhase gave two symmetric-doublet components indicating the presence of two iron species, while that of the active nhase gave a single symmetric doublet indicating the presence of a single iron species. magnetic su ... | 1992 | 1568476 |
| localization of the cholesterol oxidase in rhodococcus erythropolis imet 7185 studied by immunoelectron microscopy. | rhodococcus erythropolis imet 7185 produces an inducible cholesterol oxidase (cod) which can easily be extracted by treatment of cells with 0.1% triton x-100. the yield of the enzyme was 3.3 u/g wet wt from induced cells, which is about 5 times more than from non-induced cells. a study of the location of cod on intact cells and on ultrathin sections by means of immunogold electron microscopy revealed the following distribution, which corresponds with the biochemical results: (1) cod, which is ex ... | 1992 | 1567784 |
| construction of an insertional-inactivation cloning vector for escherichia coli using a rhodococcus gene for indigo production. | pslh8, an insertional-inactivation cloning vector for escherichia coli has been constructed by inserting a pigment gene (probably encoding an indole dioxygenase) from rhodococcus sp. atcc 21145 into puc18. wild-type e. coli colonies containing pslh8 produce insoluble indigo and turn dark blue on unsupplemented lb agar. insertion of dna fragments into the unique bamhi, ecori, ecorv, hindiii, psti, sphi and ssti polylinker cloning sites disrupts the reading frame of the fully sequenced pigment gen ... | 1992 | 1556550 |
| recurrent pneumonia due to rifampicin-resistant rhodococcus equi in a patient infected with hiv. | 1992 | 1548410 | |
| fasciation induction by the phytopathogen rhodococcus fascians depends upon a linear plasmid encoding a cytokinin synthase gene. | rhodococcus fascians is a nocardiform bacteria that induces leafy galls (fasciation) on dicotyledonous and several monocotyledonous plants. the wild-type strain d188 contained a conjugative, 200 kb linear extrachromosomal element, pfid188. linear plasmid-cured strains were avirulent and reintroduction of this linear element restored virulence. pulsed field electrophoresis indicated that the chromosome might also be a linear molecule of 4 megabases. three loci involved in phytopathogenicity have ... | 1992 | 1547783 |
| transformation of an insect symbiont and expression of a foreign gene in the chagas' disease vector rhodnius prolixus. | a shuttle plasmid was developed that is capable of replicating both in escherichia coli and in rhodococcus rhodnii, a bacterial symbiont of the chagas' disease vector rhodnius prolixus. we have been able to transform r. rhodnii with this plasmid, infect aposymbiotic r. prolixus with the transformed symbionts, select with the antibiotic thiostrepton, and re-isolate genetically altered symbionts from the insects following successive molts. symbiotic bacteria are potentially valuable as vehicles fo ... | 1992 | 1539755 |
| rhodococcus equi endobronchial mass with lung abscess in a patient with aids. | an endobronchial lesion with lung abscess in a patient with aids was due to rhodococcus equi. the patient responded to triple chemotherapy. | 1992 | 1539150 |
| in vitro activity of teicoplanin and vancomycin against gram-positive bacteria from human clinical and veterinary sources. | the minimum inhibitory concentration (mic) of teicoplanin and vancomycin was determined by the agar dilution method for 186 gram-positive bacteria from human clinical and veterinary sources. teicoplanin mic values were less than or equal to 4 micrograms/ml for 94% of staphylococci (group a, n = 52) and less than or equal to 2 micrograms/ml for all streptococci, enterococci, aerococci and pediococci (group b, n = 75). seventy-eight percent of gram-positive rods, rhodococcus and leuconostoc spp. ( ... | 1992 | 1535201 |
| [lung abscess caused by rhodococcus (corynebacterium) equi in hiv infection. two cases]. | rhodococcus (corynebacterium) equi is a well-known gram positive bacillus which is usually pathogenic in farm animals but rarely causes diseases in humans. only 30 cases of human infection have been reported in the literature. r. equi has recently been added to the list of opportunistic agents in severe hiv infection. its most common manifestation in immunocompromised is a slowly progressive pneumonia which may cavitate. r. equi pulmonary infections are difficult to treat: they usually require p ... | 1992 | 1533035 |
| analysis of the immunodominant antigens of corynebacterium pseudotuberculosis. | antibodies to seven antigens in a whole cell lysate of corynebacterium pseudotuberculosis ranging in molecular mass from 22 to 120 kilodaltons (kda) were present in sera of 40 sheep and goats infected with c. pseudotuberculosis. three antigens of about 120, 68, and 31.5 kda in size were consistently detected with sera from all animals and twenty-two sera had antibodies to 64, 43, 40, and 22 kda antigens. none of these antigens were detected by sera from 160 sheep in a c. pseudotuberculosis-free ... | 1992 | 1531563 |
| localization of the steroid 1-dehydrogenase in rhodococcus erythropolis imet 7030 by immunoelectron microscopy. | the steroid 1-dehydrogenase of rhodococcus erythropolis imet 7030, an active steroid-transforming strain, was localized by immunogold labelling both in cells induced with 17-alpha-methyl-testosterone and in noninduced cells. the labelling intensity was much higher in induced cells than in noninduced cells, indicating increased enzyme production in the case of induction. using the postembedding procedure, the main portion of the enzyme was found in the peripheral region of the cytoplasm. a consid ... | 1992 | 1527710 |
| utilization of 1,3,5-trihydroxybenzene (phloroglucinol) by a soil isolate, rhodococcus species bpg-8. | a gram-positive bacterial strain was isolated from oil rich soil in newfoundland and found to utilize various di- and trihydroxylated aromatic compounds as a source of carbon and energy. this bacterium exhibited rod/coccus dimorphism during its growth cycle. chemical analysis of cell wall composition (amino acids, sugars, and fatty acids) was performed using gas chromatography-mass spectrophotometry and high pressure liquid chromatography. comparison of both acid production and growth substrates ... | 1992 | 1527706 |
| the n-terminal amino acid sequences of brevibacterium sp. r312 nitrile hydratase. | nitrile hydratase from brevibacterium sp. r312 was purified to homogeneity. the isoelectric point was 5.75. the two kinds of subunits were separated by reverse phase hplc and their n-terminal amino acid sequences were found to be identical to those of rhodococcus sp. n-774 nitrile hydratase. | 1992 | 1527703 |
| identification, cloning and sequence analysis of the poly(3-hydroxyalkanoic acid) synthase gene of the gram-positive bacterium rhodococcus ruber. | the first polyhydroxyalkanoic acid (pha) synthase gene (phbcrr) of a gram-positive bacterium was cloned from a genomic library of rhodococcus ruber in the broad-host-range plasmid vector prk404. the hybrid plasmid harboring phbcrr allowed the expression of polyhydroxybutyric acid (phb) synthase activity and restored the ability of phb synthesis in a phb-negative mutant of alcaligenes eutrophus. nucleotide sequence analysis of phbcrr revealed an open reading frame of 1686 bp starting with the rar ... | 1992 | 1526467 |