Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| pathophysiology of chemical injury of the thyroid gland. | many goitrogenic xenobiotics that increase the incidence of thyroid tumors in rodents exert a direct effect on the thyroid gland to disrupt one of several possible steps in the biosynthesis and secretion of thyroid hormones. this includes: (1) inhibition of the iodine trapping mechanism (thiocyanate or perchlorate); (2) blockage of organic binding of iodine and coupling of iodothyronines to form thyroxine (t4) and triiodothyronine (t3) (e.g. sulfonamides, thiourea, methimazole, and aminotriazole ... | 1992 | 1281935 |
| opposite effect between intercalator and minor groove binding drug on the higher order structure of dna as is visualized by fluorescence microscopy. | fluorescence microscopy is applied to obtain the information on the change of the higher-order structure of giant dna molecules in an aqueous solution. using t4 dna, it becomes clear that ethidium bromide, an intercalator, tends to extend the dna, whereas minor groove binding drugs such as 4',6-diamidino-2-phenylindole and hoechst 33258 tend to contract the dna. these changes of higher-order structure have been quantitatively evaluated as the changes in persistent length together with the change ... | 1992 | 1280134 |
| ["risk children": the importance of biological and psychosocial risks for child development in the first two years of life]. | in a prospective longitudinal study the development of n = 362 children will be followed over four time points (t1-t4) from birth to school age utilizing a comprehensive battery of instruments. aims of the study are the description of the developmental course of children born at differing degrees of biological and psychosocial risk, the identification of early predictors of developmental disorders and their compensation, as well as the analysis of the processes and mechanisms underlying differen ... | 1992 | 1279655 |
| streptozotocin-induced decreases in serotonin turnover are prevented by thyroidectomy. | although characterized as hypothyroid, streptozotocin-diabetic rats have reduced serotonin turnover (5-hydroxyindoleacetic acid/serotonin, 5-hiaa/5-ht) in brain stem, while hypothyroid rats have increased 5-hiaa/5-ht. in the present study the two treatments were combined to determine if they affected 5-hiaa/5-ht through the same mechanism. in addition, an alternative method was used to assess 5-ht activity in thyroidectomized (tx) rats, i.e. measurement of 5-ht disappearance after inhibition of ... | 1992 | 1279445 |
| [intra-arterial compa chemotherapy for invasive bladder cancer]. | we presented 12 patients with invasive bladder cancer treated by the subselective intra-arterial compa chemotherapy. compa was administered up to an average of 3.3 courses (ranged from 2 to 6 courses) every 2 or 3 weeks, consisting of cisplatin: 15 mg/m2 on days 4 and 5; vincristine (oncovin): 0.6 mg/m2 on days 1 and 2; methotrexate: 5 mg/m2 on days 2 and 3; peplomycin: 5 mg/body on days 1, 2 and 3; and adriamycin: 10 mg/m2 on day 4. these were injected through a teflon catheter the tip of which ... | 1992 | 1279259 |
| [enzymatic probing of structural defects induced by u.v. light in phage t4 dna]. | 1976 | 1277994 | |
| the renal handling of insulin and thyroid hormones in normal man. | 1. the renal extraction of insulin and thyroid hormones t3 and t4 was studied in man by right renal vein catheterization. 2. the mean renal extraction of insulin was 0-46, suggesting that the right renal vein should be utilized in comparable studies. 3. no renal extraction of thyroid hormones was detected. | 1976 | 1277752 |
| serum triiodothyronine levels in patients receiving l-thyroxine. | serum triiodothyronine (t3) and thyroxine (t4) levels were measured in 103 adult patients receiving sodium l-thyroxine (synthroid), 0.2 or 0.3 mg daily for at least 3 mo, as suppression or replacement therapy. all were judged to be clinically euthyroid at the time of the measurements. the mean +/-sd t3 level during treatment was 130.3 +/-47.5 ng% and the mean t4 level was 11.0 +/-2.0 mug%. in normal untreated euthyroid subjects, the mean t3 level was 115.4 +/-26.0 ng% and the mean t4 level was 8 ... | 1976 | 1277707 |
| an evaluation of three commercially prepared anion-exchange resin columns for separation of tetraiodothryonine in serum. | three different commercially prepared anion-exchange resin columns for thyroxine (t4) separation from serum for the "t4 by column" assay were evaluated using the protocol of the supplier. the claims by the suppliers for the distribution and recovery of t4 in the first and second thyroxine-containing eluates were experimentally evaluated by the addition of a tracer quantity of purified [125i]t4. the average experimentally determined elution ratios of recoverable t4 (sum of t4 in first and second ... | 1976 | 1277558 |
| the distribution of functional bacteriophage t4 mrna on polysomes. | 1976 | 1275516 | |
| [congenital disorders of the thyroid gland in experimental litters obtained from mothers subjected to immunization and the action of i-131]. | i131 injected to rabbits immunized with homologous thyroid gland on the 19th-20th day of pregnancy led to congenital disturbances of the thyroid gland in the experimental litters in the i and ii generations. the functional activity and the activity of proteolytic enzymes of the thyroid gland decreased by the 30th day of the postnatal development. there was revealed a distinct correlation between the content in the thyroid gland of the experimental litters of dit + mit and of organic forms--t3 + ... | 1976 | 1273050 |
| evidence for a third type of uv. repair in bacteriophage t4. | 1976 | 1272269 | |
| identification of gene products required for in vitro formation of the internal peptides of bacteriophage t4. | in vitro formation of both bacteriophage t4 internal peptides (ii and vii) from preexisting precursor protein was shown to require the product of t4 gene 21. the proteolytic factor was detectable in extracts of cells infected with certain phage mutants blocked in early steps of head assembly but could not be demonstrated in extracts of t4 wild-type infected cells. this finding suggests that the proteolytic factor is inactivated during normal phage assembly. the product of t4 gene 22 appears to b ... | 1976 | 1271534 |
| absence of interparental recombination in multiplicity reconstitution from incomplete bacteriophage t4 genomes. | interparental recombination between injected t4 dna molecules is indetectable for incomplete petite phages (carrying a terminally deficient genome and therefore unable to circularize) as well as for genetically complete phages. the nonvialbe petite phages can individually replicate their dna repeatedly, and they aso undergo multiplicity reconstitution, producing complete phages, provided that a host bacterium is infected by several petite particles that carry genetically complementary segments o ... | 1976 | 1271533 |
| method for the isolation of bacteriphage t4 mutants that produce particles with giant capsids. | an efficient procedure is described for isolating bacteriophage t4 mutants that produce particles with giant capsids. the selective parameters of the technique rely primarily on the giants' characteristic density in cesium gradients and on their resistance to uv radiation. | 1976 | 1271524 |
| multiple structures of adeno-associated virus dna: analysis of terminally labeled molecules with endonuclease r-hae iii. | the double-stranded form of adeno-associated virus (aav) dna has about 20 sites sensitive to endonuclease r.hae iii from haemophilus aegypitus; the fragments produced fall into about 13 size classes, 8 of which contain single fragments. the location of the hae iii-produced aav fragments relative to the three ecor1 fragments was determined. using revised figures for the molecular weights of the hae iii cleavage products of phix174 replicative form dna, we calculated that aav dna contains about 4, ... | 1976 | 1271522 |
| primary structure of bacteriophage t4 internal protein ii and characterization of the cleavage upon phage maturation. | 1976 | 1271467 | |
| studies on temperature-dependent ultraviolet light-sensitive mutants of bacteriophage t4: the structural gene for t4 endonuclease v. | 1976 | 1271461 | |
| stability of radiothyroxine plasma disappearance curve despite catharsis and unblocked thyroidal uptake of radioiodide. | plasma radioactivity was measured over 21 days after an intravenous injection of 50 muci of 125i-t4 in eight normal men. no thyroid-blocking medication was given. four subjects (castor oil group) received 30 ml of castor oil on each of days 13, 14, and 15, while the other four subjects (control group) were studied without medication. after a 5-day equilibration period, plasma 125i-t4 was measured on days 5-13 in order to calculate the disappearance curve for each subject and to derive the mean f ... | 1976 | 1271113 |
| effects of postnatal thyroxine administration on brain development, response to postnatal androgen and thyroid regulation in female rats. | some developmental and functional manifestations of thyroxine (t4) administered on the first 2 days of postnatal life were studied in the female rat. brain myelinogenesis estimated by brain esterified cholesterol concentration, and brain myelin age estimated by brain total cholesterol concentration, were subsequently determined. thyroxine treatment resulted in a greater concentration of esterified cholesterol in the brain that saline treatment, but the latter appeared to delay the normal increas ... | 1976 | 1270961 |
| volume of sella turcica in normal subjects and in patients with primary hypothyroidism and hyperthyroidism. | in an attempt to assess a possible relationship between pituitary size and tsh secretion, the volume of sella turcica was measured in 570 subjects, 26 primary hypothyroid patients, and 34 thyrotoxic patients. the volume of sella turcica, measured by a 3-dimensional approach, increased progressively with age until 20 years of age and was rather constant thereafter in normal subjects. in thyrotoxic patients, the volume of sella turcica was normal in spite of decreased plasma tsh concentration. in ... | 1976 | 1270575 |
| single-stranded dna structure and dna polymerase activity in the presence of nucleic acid helix-unwinding proteins from calf thymus. | in the preceding articles we have described the isolation and some of the properties of two calf thymus proteins which bind selectively to single-stranded dna and which appear analogous to previously isolated prokaryotic dna-unwinding proteins. in the present work we demonstrate two further points of analogy. first, both the calf up1 and the high salt eluting proteins form protein-rich complexes with single-stranded dna, and hold this dna in a rigid, extended conformation. second, these proteins ... | 1976 | 1270427 |
| nucleic acid helix-coil transitions mediated by helix-unwinding proteins from calf thymus. | we have studied nucleic acid double helix destabilization mediated by purified calf helix-unwinding proteins, measuring ultraviolet hyperchromicity to detect helix melting. both calf unwinding protein 1 (up1) and a high salt eluting protein fraction are found to depress strongly the helix melting temperature (tm) of the synthetic alternating copolymers poly[d(at)] and poly[r(au)], indicating that both dna and rna are recognized by these proteins. up1 also destabilizes natural, gc-containing dna ... | 1976 | 1270426 |
| gamma-ray mutagenesis in bacteriophage t4. | 137cs-gamma irradiation of bacteriophage t4 induces large deletions plus a variety of types of point mutations. all mutations arise with single-hit kinetics, and all by a misrepair process. the estimated point mutation rate is 1.5 x 10(-9) per locus per rad. | 1976 | 1269910 |
| [cooperative binding of the protein of gene 32 of phage t4 with single-stranded dna]. | 1976 | 1269390 | |
| novel mechanism of resistance to folate analogues: ribonucleoside diphosphate reductase deficiency in bacteriophage t4. | some spontaneously occurring bacteriophage t4 mutants (far mutants) were able to form plaques in the presence of concentrations of folate analogues that completely inhibit plaque formation by wild-type phage t4. some of these far mutants were shown to be ribonucleoside diphosphate (rdp) reductase (ec 1.17.4.1) deficient, and some independently isolated rdp reductase-deficient mutants (nrd mutants) were shown to be folate analogue resistant. the map positions of the rdp reductase-deficient far mu ... | 1976 | 1267427 |
| preparation of 125i-thyroxine and 125i-triiodothyronine of high specific activity for the radioimmunoassay of serum total t4 and t3. | a simple and inexpensive procedure for the preparation of high specific activity 125i radioiodinated triiodothyronine and thyroxine is described. the specific activities achieved are approximately 350 ci/g for 125i-t4, and either 200 ci/g for 125i-t3 depending on the reagents employed. the high specific activity 125i-t4 and 125i-t3 are suitable for the routine radioimmunoassay of serum total t3 and t4. when properly stored they have a self life of at least seven weeks. | 1976 | 1267413 |
| ligandin and z protein in binding of thyroid hormones by the liver. | sephadex g-100 chromatography of rat liver supernatant after addition of [125i]t3 revealed four peaks of protein-bound radioactivity in the void volume, albumin, ligandin, and z-containing regions, respectively. the peaks were identified by cochromatography of bsp and [125i]t3 and immonodiffusion with antiratligandin igg and antirat z igg. binding of [125i]t4 to rat liver supernatant occurred in void volume, albumin, and z regions only. studies in vivo reveal a pattern of [125i]t3 binding to rat ... | 1976 | 1267008 |
| inhibitory effect of large doses of propylthiouracil and methimazole on an increase of thyroid radioiodine release in response to thyrotropin. | thyroidal radioiodine release increased shortly after a single injection of small doses of ptu, while moderate doses of mmi produced a similar increase of thyroidal radioiodine release with a latency of 7-9 hr. large doses of ptu and mmi failed to augment thyroidal radioiodine release for at least 29 to 34 hr after the initial administration of goitrogens, although plasma tsh increased significantly because of goitrogen administration. an increase of thyroid hormone release in response to exogen ... | 1976 | 1265085 |
| thyroxine and triiodothyronine influence cultured throid cells: alterations of morphology and mucosubstances. | further experimentation with the steer thyroid cell line indicates that the formation of "follicles" is enhanced by exposure to 8.9 x 10(-7) m thyroxine (t4) or triiodothyronine (t3) for 96 hr. the production of mucosubstances by the cultured thyroid cells is also increased by treating with t4 or t3, effects demonstrable after staining with pas-alcian blue at ph 2.5. it is suggested that these in vitro effects bear on morphological organization and synthetic activity of the thyroid gland as well ... | 1976 | 1265068 |
| a simple and rapid radioimmunoassay of triiodothyronine in unextracted serum. | a simple, accurate, and rapid procedure for radioimmunoassay (ria) of triiodothyronine (t3) is described. serum is denatured by sodium trichloroacetate and the t3 released is allowed to react with 125i-t3-labeled t3 anti-serum. the displaced 125i-t3 is rapidly taken up by an anionic-resin sponge, and this uptake is linearly related to serum t3 concentration. the entire assay procedure was completed in the same tube in about 1 hr. the t3 antibody was specific, and t4-to-t3 conversion in the assay ... | 1976 | 1262950 |
| triiodothyronine (t3)-binding immunoglobulins in a euthyroid woman: effects on measurement of t3 (ria) and on t3 turnover. | a 36-year-old woman with nodular goiter, nervousness, and tachycardia was evaluated for t3 toxicosis. her serum thyroxine level, resin t3 uptake, and thyroidal radioiodine uptake were normal. her t3 (ria), by a technique employing charcoal to separate bound and free t3, was reported as indeterminate due to an interfering substance; by a double-antibody method, her t3 (ria) was 325 ng/dl. further studies of the patient's serum revealed an abnormal t3-binding protein which misgrated in the beta-ga ... | 1976 | 1262440 |
| improved diagnosis and management of hyper- and hypothyroidism by timing the arterial sounds. | "sphygmo-recording," a non-invasive method for timing the arterial pulse wave contour provides an objective measure of responses to medication in patients with hyper- and hypothyroidism. the qkd interval, i.e., the interval from the onset of the qrs complex (q) to the onset of the korotkoff sounds (k) at the brachial artery when the sphygmomanometer cuff is at diastolic pressure (d) is the qkd interval. qkd is normally 205 +/- 12 msec. in the hyperthyroidism the qkd interval may be shortened to ... | 1976 | 1262433 |
| thyroidal iodine metabolism during the development of the chick embryo. | stable iodine was measured in the thyroid gland of the chick embryo from day 9 to day 20 of incubation in order to evaluate quantitatively the functional development of the gland. total iodine content increased progressively during incubation. from day 9 to day 17 of incubation, this increase resulted from the increases of pellet-bound iodine and of soluble iodine. afterwards, it essentially paralleled the increase of the soluble thyroglobulin-bound iodine which reflected the increase in both th ... | 1976 | 1261525 |
| thyroid hormone metabolism during liver regeneration in rats. | the metabolism of thyroid hormones was studied during the prereplicative period of liver regeneration. after partial hepatectomy, serum thyroxine (t4) and triiodothyronine (t3) levles progressively fell, and reached a nadir at 12 h proportional to the quantity of liver tissue exised. the diminution (60-80%) in serum iodothyronines was related specifically to partial hepatectomy because laparotomy, ether anesthesia, and other stressful surgical procedures did not induce similar changes. at least ... | 1976 | 1261517 |
| abnormal thyroid function in spontaneously hypertensive rats. | thyroid weight, thyroidal radioiodide uptake, and cyclic amp-dependent protein kinase activity of a thyroid supernatant fraction were increased significantly in spontaneously hypertensive rats (shr), apparently because of increased secretion of pituitary tsh. however, the thyroids of shr did not make supernormal amounts of thyroxine (t4), and thyroidal radioiodine release was apparently impaired. in the shr, proteolytic enzyme activity was less than normal and the thyroglobulin was more resistan ... | 1976 | 1261506 |
| a granule-associated l-thyroxine deiodinating system in the human leukocyte. | recent work has shown that leukocytes deiodinate l-thyroxine (t4) and that deiodination is enhanced when phagocytosis is induced. to define the subcellular locus and characteristics of this phenomenon, the deiodinative activities of different cell fractions obtained from human leukocytes (greater than 90% polymorphonuclear) were studied. of the several fractions obtained, the granule fraction was found to possess the bulk of t4-deiodinative activity; as little as 9 mug/ml of granule protein effe ... | 1976 | 1261501 |
| the influence of thyroxine on the serum free triiodothyronine concentration. | by means of measurement of the serum free triiodothyronine fraction (%ft3) by equilibrium dialysis, the influence of varying serum concentrations of thyroxine (t4) on %ft3 was studied in conditions associated with lack or excess of serum t4 and in serum enrichment with t4 in vitro. the enrichment of pooled serum with t4 caused significant increases of %ft3 with t4 was added to elevate its t4 content between 10 and 25 mug/dl. in 4 subjects with primary hypothyroidism treated with t3 (25 mug once ... | 1976 | 1261041 |
| purification of thyroxine and triiodothyronine for radioassay with sephadex. | the use of [125i] thyroxine in the determination of dialysable thyroxine (free t4) places very high demands on the purity of the reagent. less than 5% dissociated radioactive iodine (as iodide) is enough to influence the results [1]. for this reason several workers have developed methods for the purification of iodo amino acids used in radioassay for thyroid hormones. two techniques have been used mainly, namely dialysis of incubated tracer-serum mixture [1,2], and adsorption chromatography on d ... | 1976 | 1261035 |
| t4-endonuclease v-sensitive sites in dna from ultraviolet-irradiated human cells. | human diploid cells (wi38) were pre-labeled with 32pi, exposed to ultraviolet irradiation and then pulse labeled with [3h]thymidine. the extracted dna from these cells was subsequently treated with the t4-endonuclease v, an enzyme which specifically nicks dna strands at positions adjacent to pyrimidine dimers. sedimentation in alkaline sucrose gradients revealed that the dna synthesized after irradiation, as well as that made before, contained endonuclease-sensitive sites. our results suggest th ... | 1976 | 1259981 |
| thyroid-pituitary response to cardiopulmonary bypass. | the effects of open heart surgery on various parameters of thyroid-pituitary function were studied in 11 patients. the pattern of hormonal changes was variable. t4 concentration increased during anaesthesia and bypass, following which it returned to normal once haemodynamic stability had been regained. the pattern of change in t3 concentration did not follow consistently that of t4 and the proportional increase in concentration was not as great. there was a significant reduction in t3 concentrat ... | 1976 | 1259888 |
| a new combined thyroid hormone index based on thyroxine, triiodothyronine and resin uptake. | a new thyroid hormone index, based on measurements of thyroxine (t4), triiodothyronine (t3) and resin uptake (rt3u), is proposed. in contrast to previous thyroid hormone indices, it incorporates both t3 and t4 measurements. this combined thyroid hormone index appears to have superior predictive value in terms of clinical correlation with thyroid disease. | 1976 | 1258827 |
| [results of treatment for laryngeal carcinoma in würzburg]. | from 1960 through 1972, altogether 418 patients with laryngeal carcinoma have been treated at würzburg, 333 = 82% of these by primary radiation therapy with co-60 gamma-rays. the results are reported and analyzed in dependence on prognostic factors as tumor site and extension, formation of metastases and age of the patient. five-year survival of primarily irradiated cases from 1960 to 1967; glottic t1 = 90%, t2 = 70%, t3 -31%. supraglottic t2 = 51%, t3 =20%, t4 = 16%. quota of cases with posteri ... | 1976 | 1258083 |
| production and evaluation of high-quality thyroxine antisera for use in radioimmunoassay. | high-quality thyroxine (t4) antisera were raised in rabbits. conjugates of protein and t4 or t4 methyl ester were given intracutaneously in freund's complete adjuvant at one or two sites on the back at 1/2- to 3-month intervals. eleven of 16 immunized rabbits produced antiserum with titers higher than 2 commercial antisera and 4 antisera from other laboratories. all 16 antisera had equilibrium constants (k) higher than the 6 reference sera. the highest titer observed was 130,000 with 88 fmol lab ... | 1976 | 1257697 |
| t4 gene 32 protein model for control of activity at replication fork. | limited hydrolysis of gene 32 protein by various proteinases results in the production of three stable cleavage products. two of these products show an affinity for native t4 dna cellulose that the uncleaved protein does not exhibit. a model for proteolytic cleavage and for the total unwinding of dna in advance of the replication fork is discussed in terms of this unusual binding affinity. | 1976 | 1256542 |
| head morphologies in bacteriophage t4 head and internal protein mutant infections. | escherichia coli infected with phage t4 mutants defective in synthesis of the three major internal proteins found in the phage head, ipi-, ipii-, ipiii-, or ip degrees (lacking all three) were examined in the electron microscope for head formation. infection with ipi- or ipii- does not appear to induce increased aberrant head formation, whereas ipii- or ip degrees infections result in production of polyheads and viable phage. multiple mutants of the early head formation genes 20, 21, 22, 23, 24, ... | 1976 | 1255864 |
| late events in t4 bacteriophage production. ii. giant bacteriophages contain concatemers generated by recombination. | analysis of "giant" phage, which package concatenated dna into their capsids, shows that they are predominantly heterozygous. the results are compatible with the hypothesis that concatemers are generated by recombination. | 1976 | 1255859 |
| late events in t4 bacteriophage production. i. late dna replication is primarily exponential. | the possibility of a switch in the mechanism of t4 dna replication, from an exponential-bidirectional mode at early stages to a nonexponential (rolling circle) mode at later stages of phage development, has been investigated. the conclusion that dna replication does not involve such a change in mechanism for the majority of replicating molecules is based on the analysis of the clonal distribution of mutants specifically induced at late times after infection. the clonal distribution of mutants, i ... | 1976 | 1255858 |
| gene 32 protein of bacteriophage t4 moderates the activities of the t4 gene 46/47-controlled nuclease and of the escherichia coli recbc nuclease in vivo. | genes 46 and 47 of phage t4 control a nuclease that is required for genetic recombination and may act similarly to the escherichia coli recbc nuclease. in vivo, the nucleolytic activities of both of these nucleases must be moderated so that recombining dna intermediates are not destroyed. we conclude from our present experiments that the phage t4 gene 32 protein, specifically its c-terminal domain, participates in such moderation. we have investigated dna degradation in different gene 32 and gen ... | 1976 | 1255856 |
| recovery of the accumulation ability of thiomethyl-beta-galactoside in escherichia coli after bacteriophage t4 infection. | effects of uv-irridiated and unirradiated t4 phage infection on the beta-galactoside accumulation ability in eschericia coli have been examined by the use of 14c-labeled thiomethyl-beta-galactoside (tmg). under conditions where a synchronous adsorption of phage takes place, the cellular ability for tmg accumulation is found to be largely inhibited immediately after phage adsorption, but it recovers with time to a new level, which is dependent on the multiplicity of infection. when cells are infe ... | 1976 | 1255845 |
| measurements of free thyroxine: comparison of per cent of free thyroxine in diluted and undiluted sera. | the effect of dilution on values of the per cent of free t4 in serum (%ft4) measured by equilibrium dialysis, was determined by comparing the values for %ft4 in diluted and undiluted specimens. the specimens studied were sera obtained from 10 normal subjects, 10 patients with hyperthyroidism, 5 with hypothyroidism, and 9 women with normal pregnancy. the method of analysis employed was that described by sterling and brenner, except that the 125i-thyroxine used was predialyzed to remove non-thyrox ... | 1976 | 1254695 |
| triiodothyronine and thyroxine in urine. ii. renal handling, and effect of urinary protein. | mean urinary clearances of t3 were 164 ml/min in normal subjects, 177 in pregnancy, 221 in thyrotoxicosis, 174 in hypothyroidism, and 194 in 3 persons with undetectable t4 but normal t3 levels. t4 clearances were 38 ml/min in normal subjects, 48 in thyrotoxicosis, and 138 in hypothyroidism. low creatinine clearance was associated with low clearances of t4 and t3. the data suggest urinary excretion of t3 by glomerular filtration of serum unbound t3 with added tubular excretion; and t4 excretion b ... | 1976 | 1254691 |
| triiodothyronine and thyroxine in urine. i. measurement and application. | urinary triiodothyronine (t3) and thyroxine (t4) were measured by ria, and t4 was also measured by competitive protein binding (cpb). ph 1-hydrolysable conjugates were 48% of total urinary t3, and enzyme- or ph 1-hydrolysable conjugates were 55% and 61% of total urinary t4. the mean unconjugated t3 excretion was 34.3 ng/h (0.99 mug t3/g creatinine) in normal subjects (no day-night rhythm found), 1.56 mug/g in late pregnancy, 0.82 mug/g in neonates (1-12 days), and was also unchanged in persons w ... | 1976 | 1254690 |
| immunogenic properties of bacteriophage spo1 and t4 dna photooxidized in the presence of methylene blue, irradiated with ultra-violet light or containing 5-bromdesoxyuridine. | 1976 | 1254318 | |
| hormonal effects on the morphology of bone defect healing. | standard defects were produced in the radii of hypophysectomized immature rats. all animals were injected with methimazole, and grouped according to hormone supplementation, growth hormone (gh), thyroxine (t4), the combination, or no hormones. lack of gh and t4 caused a retardation of defect healing and callus formation but not a total cessation. the presence of gh in the hypophysectomized animal caused a larger callus due to greatly increased chondrogenesis throughout the 6 week study. thyroxin ... | 1976 | 1253491 |
| [unusually high iodine excretion in hyperthyroidism in an iodine-poor area]. | in 27 thyrotoxic patients and in 177 healthy controls from the area of essen we studied the total urinary iodine excretion. whereas the iodine excretion was only 43.7 +/- 27.3 mug iodine per g creatine in the controls, the corresponding average value was significantly higher in thyrotoxic patients. it amounted to 193.3 +/- 201.7 mug iodine per g creatinine. this finding can not be explained by selection of patients with autonomous adenomas and contamination with iodine. possible causes are an in ... | 1976 | 1251155 |
| total and free thyroxine and triiodothyronine in normal and complicated pregnancy. | serum concentrations of total and free thyroxine (t4 and ft4) and total and free triiodothyronine (t3 and ft3) were measured in normal pregnant women, in patients with toxemia of pregnancy, and in patients with gestational trophoblastic disease (gtd). in normal pregnancy, ft4 and ft3 levels remained normal while t4 and t3 levels were elevated. in patients with pre-eclampsia, the mean serum t3 concentration was significantly lower than that of normal pregnancy and the serum ft3 concentrations in ... | 1976 | 1249198 |
| hyperthyroidism with normal concentrations of total serum thyroxine and triiodothyronine. | normal concentrations of total serum thyroxine (t4) and triiodothyronine (t3) were found in a patient who was hyperthyroid because of a hyperfunctioning thyroid adenoma. after surgical removal of the adenoma, the patient became clinically euthyroid; the abnormally high free thyroxine (ft4), triiodothyronine resin uptake (t3r) and rapid achilles reflex time (art) returned to normal. a low-normal concentration of thyroxine binding globulin (tbg) determined by polyacrylamide gel electrophoresis and ... | 1976 | 1249194 |
| pituitary-thyroid axis reaction after myocardial infarction. | within the first 36 hours following myocardial infarction, serum total thyroxine (t4) levels were supranormal in most cases in contrast to normal thyrotropin values. after one week, t4 levels dropped to normal while tsh values rose significantly. these findings suggest that, in the acute phase of myocardial infarction, the secretion of thyroid hormones is increased, thereby inhibiting the pituitary thyrotropes. the stimulation of thyroid secretion might be due to the high levels of blood catecho ... | 1976 | 1248789 |
| incorporation of (5-3h) uridine into ribonucleotide pools and rna during thyroxine-induced metamorphosis of xenopus laevis tadpoles. | incorporation of [3h] uridine into the ribonucleoside triphosphates utp and ctp, total rna, and nuclear and cytoplasmic rna was followed in xenopus laevis tadpole liver during thyroxine (t4)-induced metamorphosis. pool sizes of utp and ctp were found to remain unchanged, although turnover the ribonucleoside triphosphates was found to be greatly stimulated after 4 days of hormone treatment. the time course of labeling of the 40-s pre-rrna was very similar to that of utp in both thyreostatic and t ... | 1976 | 1248666 |
| a dna polymerase from ustilago maydis. 2. properties of the associated deoxyribonuclease activity. | the polymerase and deoxyribonuclease activities of the purified ustilago maydis dna polymerase coeluted from a hydroxyapatite column, cosedimented in sucrose gradients in both the absence and presence of salt, possessed similar thermolabilities and reaction requirements. these observations suggest that both activities are associated with the same enzyme and that the deoxyribonuclease activity is not a contaminant. the initial rate of degradation of native 3'-end-group-labelled dna was similar to ... | 1976 | 1248476 |
| thyroid hormone regulation of extrathyroidal iodoproteins. | the effect of thyroid status on plasma and tissue levels of labeled nonextractable iodine (nei) derived from the metabolism of radioiodothyronines was examined in the rat. concentrations of radioiodoprotein were substantially elevated in plasma, kidney, and liver in thyroidectomized animals 72 h postinjection of [125i]triiodothytonine ([125i]t3). similarly, total rat concentrations of radioactive nei were increased (52%) 72 h after injection of [125i]t3. ne125i concentrations from [125i]t3 in pl ... | 1976 | 1248455 |
| conversion of l-thyroxine to triiodo-l-thyronine and biological activity of l-thyroxine, as measured by changes in growth hormone. | pituitary growth hormone (gh) content and plasma gh response to pentobarbital (pb) of severely hypothyroid rats are markedly decreased compared with euthyroid rats. both pituitary and plasma gh may be increased by single ip injections of physiologic doses of thyroxine (t4) (1.75 mug/100g bw) or triiodothyronine (t3) (0.2 mug/100g bw). using this increase in gh of hypothyroid rats to measure the biological effectiveness of single doses of both iodothyronines, we have shown that the administration ... | 1976 | 1248450 |
| cooperative action of the t4 tail fibers and baseplate in triggering conformational change and in determining host range. | 1976 | 1246820 | |
| results of interstitial curietherapy in t3 and t4 floor of the mouth and tongue cancers after previous arterial infusion chemotherapy. | 1976 | 1246449 | |
| starvation-induced alterations of circulating thyroid hormone concentrations in man. | serum concentrations of triiodothyronine (t3), thyroxine (t4), and tsh were examined in seven men and seven women of normal weight during a 60-hr fast. similar studies were conducted in two women who received daily for 1 mo before and during a similar fast, 0.4 mg and 0.5 mg of 1-thyroxine. the serum concentrations of t3 decreased in each of the untreated normal subjects (sign test of significance, p less than 0.001). the mean control concentration of t3 in women was 152 +/- 9 ng/100 ml (x +/- s ... | 1976 | 1246209 |
| [clinical studies on autoimmune mechanisms of the thyroid. part 2. studies on measurements of serum thyroblobulin concentrations in patients with various thyroid diseases (author's transl)]. | the present report deals with the measurement of serum thyroglobulin (tg) in various thyroid disorders by using radioimmunoassay (ria). the results were as follows: 1) a specific and simple solid-state ria for the measurement of tg in human serum was used. this system was a direct ria using plactic cups coated with crude anti-thyroglobulin antibodies (anti-tg) and 125i labeled purified anti-tg. the purification of anti-tg was performed by affinity chromatography using tg-sepharose, as an immunoa ... | 1975 | 1243914 |
| high incidence of chronic lymphocytic thyroiditis in apparently healthy school children: epidemiological and clinical study. | an epidemiological survey on the incidence of juvenile chronic lymphocytic thyroiditis was performed in 10,220 apparently healthy school children in ishikawa district, japan. the subject of present study included 6,244 school children (2,831 boys and 3,413 girls, ages 6-18 yrs.) in kanazawa city and 3,976 children (2,055 boys and 1,921 girls, ages 6-18 yrs.) in wajima city. the first group was selected as a representative of urban area and the second group as that of seaside area. children who h ... | 1975 | 1243913 |
| [variation of the thyrotropin-releasing-hormone (trh) stimulated thyrotropin (tsh) response in comparison with the tyhroid-gland-suppression test and the triiodothyronine (t3) and thyroxine (t4) blood levels in the so-called euthyroid endocrine ophthalmopathy]. | 21 patients with active signs of euthyroid graves' disease were given 400 mug thyrotropin-releasing hormone (trh) i.v. all subjects with unresponsiveness to trh had a nonsuppressible thyroidal 131i-uptake. on the basis of serum total t3 14 patients were hyperthyroid, 2 more had an elevated value of free t3. 4 patients with normal total t3 and nonsuppressible 131i-uptake were unresponsive to trh, in 2 of them the free t3 fraction was elevated, however. 4 subjects with nonsuppressible 131i-uptake ... | 1975 | 1243821 |
| solitary hyperfunctioning thyroid adenoma in a child. elevated t3 and t4 levels with normal tsh level. | 1975 | 1242753 | |
| opposite effects of dexamethasone on serum concentrations of 3,3',5'-triiodothyronine (reverse t3) and 3,3'5-triiodothyronine (t3). | dexamethasone, 2 mg every 6 hours for 4 doses, was given to 4 hypothyroid patients receiving treatment with synthetic thyroxine (t4) and to 8 untreated hyperthyroid patients with graves' disease, and serum concentrations of thyroid hormones were measured by radioimmunoassays. serum concentration of 3,3'5'-triiodothyronine (reverse t3, rt3) increased appreciably within 8 hours after the first dose of dexamethasone, was maximum at 24-32 hours after beginning dexamethasone, and remained elevated fo ... | 1975 | 1242390 |
| nyctohemeral and sex-related variations in plasma thyrotropin, thyroxine and triiodothyronine. | tsh, t4, and t3 were measured by radioimmunoassay in plasma samples obtained from 77 young adult male and 114 female rats fed a purina high-iodine diet and maintained in an isolated room, 2-4/cage, at 24 +/- 1 c with light from 0600-1800 h. in one experiment, 7 male and 7 female rats were decapitated every 3 h for 30 consecutive h and trunk blood was collected. there was a clear nyctohemeral rhythm of plasma tsh in both sexes characterized by a zenith at 1200 h and a nadir between 1800 and 2100 ... | 1975 | 1239368 |
| bacteriophage t4 baseplate components. i. binding and location of the folic acid. | two different proteins with high affinities for the pteridine ring of folic acid have been used to determine the location of this portion of the folate molecule in the tail plate of t4d and other t-even bacteriophage particles. the two proteins used were (i) antibody specific for folic acid and (ii) the folate-binding protein from bovine milk. both proteins were examined for their effect on various intact and incomplete phage particles. intact t2h was weakly inactivated by the antiserum but not ... | 1975 | 1238580 |
| inactivation of hapten-modified bacteriophage by antibody: increased sensitivity of the assay in media containing polyethylene glycol. | inactivation of dinitrophenylated bacteriophage t4 by porcine and rabbit anti-dinitrophenyl (dnp) antibodies was studied by the complex inactivation methods. the inactivation titers increased 20-30 times when polyethylene glycol, mol. wt. 6000, was added to the incubation media. consequently, the detection of dinitrophenylated serum albumin with the modified bacteriophage could be carried out with higher sensitivity upon addition of polyethylene glycol. | 1975 | 1234056 |
| [the influence of proteins bound to single-stranded dna on rna and poly(a) synthesis. i. antibodies against dna]. | the influence of antibodies to dna of t4 and spoi phages as well as to dna of e. coli on rna and poly(a) synthesis by e. coli rna polymerase has been studied. antibodies to the phage dna specifically inhibit rna and poly(a) synthesis by 30 to 60% only in case of homologous dna, the stage of elongation being inhibited predominantly. a comparison between the effects of the antibodies upon different stages of rna synthesis on the native dna and on the synthetic oligonucleotide template (dt)12 allow ... | 1975 | 1228470 |
| [effect of mutant rna polymerase on spontaneous mutagenesis in bacteriophage t4]. | the frequency of spontaneous reversion in r-mutants of phage t4 is determined in order to study the influence of mutant rna polymerase on the level of spontaneous mutations in phage t4. the rate of spontaneous mutagenesis in the phage is found to be increased in strains with mutant rna polymerase. | 1975 | 1228060 |
| further studies on prostaglandins. iii. effect of prostaglandins on thyroid activity as assessed by 125i. | the effect of crude prostaglandins extraced from prostate glands of camels as well as that pge1, on the thyroid activity of male immature boskat rabbits, was investigated. the iodinated amino acid fractions (t1, t2, t3 and t4) in the serum of the injected groups were determined in control and treated animals. cytological work on sections of the thyroid glands of the treated and control rabbits were performed to study the effect on subcellular level. the results indicate that both crude and pure ... | 1975 | 1227829 |
| myocardial high energy phosphates in experimental thyrotoxicosis in guinea pigs. | experimental thyrotoxicosis was produced in guinea pigs by daily intraperitoneal injection of 0.7 mg/kg thyroxine (t4) or 0.15 mg/kg triiodothyronine (t3), as shown by an increase of pbi over 50 gamma percent, weight reduction of 20--30 percent, and increase in heart rate of 30--50 percent. control animals received solvent only. metabolites were determined in heart muscle on the 1st, 3rd, and 7th day using freeze-stop technique and enzymatic methods: creatine phosphate (cp), atp, adp, amp, gluco ... | 1975 | 1226434 |
| pre-operative short intensive radiation therapy of t3-t4 laryngeal carcinoma. | a group of 33 patients with t3-t4 laryngeal carcinomas received in the period 1968 to 1972 pre-operative irradiation with 60co followed by surgery. a total dose of 2 500 rad was given in 5 equal sesssions. the tumour free survival rates of these patients are compared to those of a comparable group of 57 patients receiving radiation therapy only. the overall results in the pre-operative group (64%) are superior to those in the group irradiated only. in glottic tumours the results were similar in ... | 1975 | 1224993 |
| low dose irradiation in advanced tumours of head and neck. | a material of 19 patients with t3 and t4 tumours of the mouth and pharynx was treated by fractionated low dose rate irradiation. regression occurred in a large proportion of tumours but at the expense of a high rate of necrosis. | 1975 | 1224991 |
| [correlations between clinical control and histopathologic findings in breast cancer after irradiation and radical mastectomy (author's transl)]. | 154 cases of breast cancer (30 t1-52; 124 t3-54) treated by irradiation followed by radical mastectomy were subjected to histological revision in order to check the efficacy of radiotherapy in the local control of cancer and to seek correlations between the histological findings and the clinical course of the disease. the tumor disappeared completely only in 24% of cases, the percentage being higher in the initially smaller tumors; the axillary lymph nodes showed disappearance of the metastases ... | 1975 | 1224390 |
| the influence of dietary iodine on lithium blood level, serum t4 and thyroid gland weight. | lithium is both antithyroid and goitrogenic. the relationship between lithium and iodine has been assessed in rats fed diets containing different iodine content. chronic lithium treatment caused increase in thyroid gland weight in animals on low and high iodine diets. a reciprocal relationship surfaced between serum lithium levels and dietary iodine content. animals on low-iodine diet had lower serum lithium levels. the data support other evidence that lithium likely inhibits organification of i ... | 1975 | 1224012 |
| [radioimmunologic determination of plasmatic triiodothyronine. verification of operative parameters; 1st clinical results]. | the experimental determination of the operative parameters of two radioimmunoassay (ria) systems for the determination of triiodothyronine (t3) level directly in serum is described. the two systems differ both for the agents blocking the aspecific t3-serum protein bindings (sulphonic acid, 8-aniline, 1-naphtalene in borate buffer: bor-ans-ria and merthiolate in phosphate buffer: po4-merth-ria) and for the methods adopted for compensating the aspecific serum interferences (t3,4 free serum for bor ... | 1975 | 1223940 |
| [deiodase activity in rats following an overdose of thyroid hormone]. | the l-dit and l-t4 deiodinating activity in supernatants from liver or kidney homogenates of normal rats or rats loaded with t4, t3 or tsh, was investigated. deiodination of l-dit occurs in liver supernatants twice as much as in liver, referring to mg of protein of g of tissue, while the deiodination of l-t4 seems rather equal. the over all rate of deiodination, however, reaches in liver for l-dit the threefold, for l-t4 the fivefold value, as compared with kidney. short periods of loading do no ... | 1975 | 1222735 |
| [absorption and pharmacokinetics of large doses of 1-thyroxine in man (author's transl)]. | in recent years suppression tests with a single dose of 3 mg 1-thyroxine (t4) and weekly doses of 1 mg t 4 in the treatment of hypothyroidism have been put to clinical trial and the lack of side-effects of such high doses (5 to 15 x daily requirements) was stressed. hence, it was decided to study the absorption of 1-t-4 from the gastrointestinal tract and its metabolism in euthyroid patients. doses from 250 to 2500 mug t 4, mixed with 250 muci 131i-thyroxine were given to 10 patients. after the ... | 1975 | 1221631 |
| [serum concentration of triiodothyronine in autonomous thyroid gland adenoma]. | serum concentration of t3 was measured in 14 patients with autonomous thyroid adenoma using a chromatographic method. 9 patients had increased levels of t4, 5 were normal. in both groups serum-t3-concentration was significantly elevated. in patients with normal t4-values hypertriijodothyroninemia seems to be the biochemical base for clinical signs of hyperthyroidism. the pathogenetical importance of elevated t3-levels is discussed for t3-secretion and t4/t3 conversion. | 1975 | 1220518 |
| [studies in the diagnosis of breast disease (author's transl)]. | the relative diagnostic accuracy of clinical examination, mammography, thermography and ultrasound was investigated in a comparative study. mammography proved to be the most accurate diagnostic method, followed by clinical examination, thermography and ultrasound. whereas in cases of advanced cancer (t2 to t4 tumour diameter greater than 2 cm) the correct diagnosis was made most reliably by clinical examination, mammography was superior to all other procedures in t1 tumours and in impalpable tu ... | 1975 | 1220258 |
| normal values of thyroxine and triiodo-thyronine retention in the rat. valores normais de tiroxina e retenção de triiodo-tironina em ratos. | the authors report the results from murphy and pattee's method 9, 10 1964, 1966 for t4 and those from hamolsky et al.'s method 5 1957 for percent retention of t3 as applied to the normal rat serum. for that purpose, 32 adult wistar rats, of both sexes, kept at the laboratory environment and fed with usual diet were used. blood samples were taken after noon directly from the heart, without anesthesia. the analysis showed a t4 value of 4.20 +/- 0.92 mug/100 ml of serum (both sexes). the percentual ... | 1975 | 1219925 |
| [tsh and t4-level in newborn infants with congenital struma]. | 1975 | 1219466 | |
| [serum level of thyroxin-binding globulin under oral contraception (author's transl)]. | the use of oral contraceptives results in an increase in thyroid-binding-capacity in conformity with a change of results by t3/t4-tests. using laurell-electrophoresis and an anti-serum produced by behringwerke the determination of the thyroid-binding-globulin (tbg) in serum is easy to handle. tbg was determined in serum from 137 women using oral contraceptives for more than 3 months. the apllication of oral contraceptives is followed by a statistically significant increase in the tbg level in se ... | 1975 | 1219185 |
| [fifty cases of penile tumors treated at the claudius-regaud center from 1958 to 1973]. | from the study of this series of 50 patients suffering from cancer of the penis, and in particular from the comparison of two successive groups, several facts stand out which determine our present attitude: -subjects suffering from cancer of the penis, at least in our area, are aged, fragile people, whose cause of death, in more than half of the cases, was not the penile cancer. proposable therapeutic methods must take this specific background into account; -tumoral and glandular indications mus ... | 1975 | 1219091 |
| differential synthesis of proteins by t4 phage & its head protein amber mutant. | 1975 | 1218878 | |
| [effect of palladium and platinum salts on bacteriophage t4 and its isolated dna]. | the effect of palladium and platinum salts (k2pdcl4, k2ptcl4) on bacteriophage f4 and its isolated dna in genetic transformation is investigated. both compounds efficiently inactivated the phage and decreased the transforming activity of donor dna. the palladium salt exhibited the higher activity. the palladium compounds inhibited the transforming activity of native donor dna to a greater degree. no difference was observed in the degree of inactivation of the transforming activity of native and ... | 1975 | 1218722 |
| [recombination in amber-mutants of bacteriophage t4b. i. recombination and complementation in phage t4 amber-mutants]. | in studying intergenic and intragenic complementation in amber mutants in genes of phage t4 controlling the synthesis of phage tail fibres the data have been obtained indicating the dependency of the results of complementation tests on those of crosses of respective markers. the results obtained show that in complementation of amber mutants of phage t4 the phage yield varies widely and depends on the location of markers on the phage genetic map. | 1975 | 1218721 |
| [the euthyreotic hypotriiodothyroninemia]. | in 33 patients with various, mainly severe diseases a subnormal triiodothyronine concentration (t3) and a normal thyroxin concentration (t4) were found. in addition to t3 and t4, the plasma tsh levels and t3 binding capacity were determined. in no case did the patient's history or clinical findings afford evidence of hypothyroidism. diminished conversion of t4 to t3 is discussed as a possible cause of the inappropriately depressed t3 levels. | 1975 | 1215959 |
| l-thyroxine and monoamine oxidase activity in the kidney and some other organs of the rat. | l-thyroxine (t4) (10,30 and 300 mug/kg. s.c. for 5 days) increased kidney monoamine oxidase (mao) activity and decreased that of the liver, using tryptamine as the substrate. these changes were dose-dependent. time-dependent changes were found with the 30 mug/kg dose. heart mao activity was increased by the high dose while small decreases were found with the lower doses. no changes were found in the whole brain, vasa deferentia, the thyroid or adrenal glands. hypophysectomized rats showed a 50% ... | 1975 | 1215664 |
| dose-relation of thyroxine-induced changes in myocardial energy stores. | experimental hyperthyroidism was produced in guinea pigs by daily intraperitoneal injection of l-thyroxine (t4) in various doses (0.7, 0.35, 0.175, and 0.07 mg/kg/day) for 7 days. controls received solvent only. the following metabolites were determined in heart muscle (freeze-stop technique, enzymatic tests): pi, adenosine tri-, di-, and monophosphates (atp, adp, amp), creatine phosphate (cp), glucose 6-phosphate (g-6-p), fructose diphosphate (fdp), pyruvate, and lactate. thyroxine induced a do ... | 1975 | 1215645 |
| [letter: progress in thyroid function testing. radiologic determination of thyroxine (t4-r)]. | 1975 | 1215157 | |
| [study of dna melting in the region of the inversion of relative stability of at and gc pairs]. | systematic data on the dependence of the melting curve parameters of dna from different organisms on the concentration of salt (c2h5)5nbr have been obtained. the melting curves were studied by spectrophotometric as well as by microcalorimetric methods. the dna melting range width is shown to pass through the minimum value delta0t = 0.6 +/- 0.1 degrees at the point of inversion of relative stability of at and gc pairs that corresponds to the concentration of (c2h5)4nbr equal to 2.9 +/- 0.1 m. thi ... | 1975 | 1214815 |
| [secondary structure of dna from t4 and t6 phages]. | the secondary structure of nadna from e. coli t4 and t6 phages has been studied by the x-ray diffraction method. molecules of these dnas as well as t2 phage dna molecules contain hydroxymethylcytosine glucosylated at different position instead of cytosine. at high relative humidity these dnas are shown to exist in b-conformaion. as humidity decreases the transformation into t=conformation takes place in the t4 phage dna whereas in the t6 phage dna changes of secondary structure similar to b-t tr ... | 1975 | 1214798 |