Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| purine nucleotide content in human t4 and t8 lymphocyte subpopulations from normal subjects and aids patients. | 1992 | 1362548 | |
| the inflammatory myelinopathy of adreno-leukodystrophy: cells, effector molecules, and pathogenetic implications. | prominent inflammation in the demyelinative lesion of adreno-leukodystrophy (ald) has suggested an immune-mediated pathogenetic component. commercially available antibodies to t cells, b cells, macrophages, class i and ii molecules, complement, igg, igm, iga, interleukin-1 (il-1), intercellular adhesion molecule-1 (icam-1) and tumor necrosis factor-alpha (tnf) were applied to paraffin sections of formaldehyde-fixed postmortem samples. twenty-five primary demyelinative lesions from five juvenile ... | 1992 | 1362438 |
| thyroid function tests are rarely abnormal in patients with severe hyperemesis gravidarum. | there is considerable controversy in the literature as to the cause of hyperemesis gravidarum. the aim of this project was to measure a range of thyroid hormone levels in a group of hyperemetic pregnant women. | 1992 | 1362375 |
| spin echo nuclear magnetic resonance studies on intact erythrocytes: changes in cellular metabolism as a consequence of carbimazole therapy. | because the exact mechanism of action of carbimazole is uncertain, nuclear magnetic resonance (nmr) spectroscopy was used to investigate cellular changes in erythrocytes from graves' patients following a course of carbimazole therapy. | 1992 | 1362374 |
| prevalence and specificity of lymphocytotoxic antibodies in different stages of hiv infection. | sera obtained from 27 hiv-infected persons were investigated for complement-dependent humoral cytotoxicity. uninfected as well as htlv-iiib-infected h9 cells were used as cellular targets either before or after stimulation by phytohemagglutinin (pha) or concanavalin a (con-a). the degree of cytotoxicity was determined by 51cr-release assay. two different antibodies could be found in sera of hiv-infected persons, one being directed against hiv-induced cell surface component(s) and the other react ... | 1992 | 1362323 |
| evidence for the kidney as an important source of 5'-monodeiodination activity and stimulation by somatostatin in oreochromis niloticus l. | radioimmunoassay of 3,5,3'-triiodothyronine (t3) and thyroxine (t4) in the thyroidal region of mature male oreochromis niloticus revealed stores of t4 but negligible levels of t3, yielding a very low t3/t4 ratio (0.3%). 5'-deiodination (5-d) of t4 into t3 was examined in liver and kidney homogenates in vitro by radioimmunoassay of t3 with t4 as substrate. in both organs, the 5'-d activity was temperature dependent: at 4 degrees c, t3 production was below the level of detection and maximal in bot ... | 1992 | 1362169 |
| a comparison of t4:t8 lymphocyte ratio in the periodontal lesion of healthy and hiv-positive patients. | previous reports describe a characteristic, rapidly progressive, periodontitis that is unique to patients who are seropositive for hiv antibody (western blot +). the purpose of this study was to compare the t4 and t8 lymphocyte subpopulations in the peripheral blood and periodontal lesions of these hiv patients with those of healthy controls. t-cell subsets in peripheral blood were quantified by flow cytometry. the values from this analysis were used to calculate the peripheral t4:t8 lymphocyte ... | 1992 | 1361945 |
| effects of erythropoietin-induced hemopoiesis on peritoneal transport and on in vitro t cell response in capd. | in order to investigate the therapeutic efficacy of subcutaneously administered erythropoietin (rhuepo) and the effects of rhuepo-induced hemopoiesis on peritoneal transport and on cellular immune responses, we performed standardized peritoneal equilibration tests and measured t cell subsets and phytohemagglutinin (pha)-induced interleukin-2 receptor (il-2r) expression of pbmc by flow cytometry before and after subcutaneous rhuepo (eprex-, cilag), 4000 u twice weekly, in 13 stable capd patients. ... | 1992 | 1361846 |
| evaluation of cns-function in capd patients using magnetoencephalography (meg): comparison with hemodialysis patients. | in order to evaluate the cns-function of uremic patients, the magnetic activity emitted from the brain of 20 pts (10 pts on capd and 10 on hd) was measured. meg consisted of taking 32 consecutive records from the 32 equally spaced points chosen on the skull in uremic pts around our reference points t3, t4, p4, f3, f4 of the international 10-20 electrode placement point system. meg data were converted using an ad-converter with sampling frequency 256 hz and stored in a p/c. our results showed sig ... | 1992 | 1361782 |
| [various indicators of the hormonal profile in patients with neuroses after psychopharmacotherapy and functional test with sinacten]. | studies of adenohypophyseal hormones, cortisol, t3, t4, thyroxine-binding globulin, testosterone, progesterone and estradiol in 90 neurotic men, treated or not with amitriptyline and benzodiazepines, allowed a conclusion that in the majority of them, endocrine functions were activated. 11 healthy men and 11 male patients underwent the three-day test with sinacten-depot (1 mg intramuscularly). the functional test data were compared over time by the methods of nonparametric statistics. the patient ... | 1992 | 1360737 |
| longitudinal study of serum thyroid hormone levels during normal pregnancy. | we undertook a prospective longitudinal study of thyroid function in 60 normal pregnant women and measured serum concentrations of t4, triiodothyronine (t3), t-uptake, thyroxine binding globulin (tbg), free thyroxine index (fti), free t4, albumin and thyrotropin (tsh). from these data we established reference ranges for each of these analytes for each trimester and examined the inter-relationships between laboratory measurements of thyroid function tests. we observed significant increases in ser ... | 1992 | 1360418 |
| role of cd11a/cd18-cd54 interactions in suppression of human b cell responsiveness by cd4+ suppressor t cells. | the role of leukocyte function-associated ag-1 (lfa-1, cd11a/cd18) and intercellular adhesion molecule 1 (icam-1, cd54) interactions in the suppression of human b cell function by immobilized anti-cd3-activated cd4+ t cells was examined by studying the effects of mab to these determinants. the suppressive activity was assessed by the effects of cd4+ t cells without mitomycin c treatment activated by immobilized anti-cd3 for 72 hr on the differentiation into ig-secreting cells of b cells activate ... | 1992 | 1360336 |
| the management of hyperthyroidism due to graves' disease in the former ussr in 1991: results of a survey. | a survey of the current management of graves' disease was performed in the ussr among members of all union endocrine society. the questionnaire was based on the format used previously for a survey of members of european thyroid association. the aim of a similar survey in the ussr was to obtain a comprehensive pattern of management of graves' disease in soviet endocrinology clinics and to compare medical attitudes in the former ussr to those in other european countries. one hundred and twenty que ... | 1992 | 1360021 |
| basic biochemical data on blood from antarctic weddell seals (leptonychotes weddelli): ions, lipids, enzymes, serum proteins and thyroid hormones. | 1. standard laboratory values of blood samples taken from weddell seals in antarctica were determined. 2. numerous blood parameters are similar to those observed in man. 3. comparatively high cholesterol levels but normal triglyceride levels were observed when compared with humans. 4. in comparison to laboratory findings in humans, t4 levels were decreased although t3 levels were normal. 5. the levels of alkaline phosphatase are considerably higher than those in humans. 6. the data obtained indi ... | 1992 | 1359937 |
| tetanus-induced changes in apparent recovery after bolus doses of atracurium or vecuronium. | the current study evaluated the duration and magnitude of post-tetanic effects in 56 patients recovering from a bolus dose of nondepolarizing relaxant to assess the impact of tetanus on monitoring in a common clinical setting. after induction of general anesthesia (thiopental, fentanyl, oxygen, nitrous oxide, and isoflurane), a baseline response to train-of-four (tof) stimulation was recorded using an adductor pollicis force transducer, and the ratio of the fourth response (t4) to the first (t1) ... | 1992 | 1358004 |
| regional distribution of pyroglutamyl peptidase ii in rabbit brain, spinal cord, and organs. | pyroglutamyl peptidase ii (ppii) is a narrow specificity ectoenzyme that degrades thyrotropin-releasing hormone (trh). we detected the enzyme in the brain of various mammals, with highest specific activity in rabbit brain. in this species, activity was heterogeneously distributed in the central nervous system. there was a 28-fold difference between regions of highest and lowest ppii activity. enzyme activity was highest in the olfactory bulb and posterior cortex. in the spinal cord, activity was ... | 1992 | 1357633 |
| participation of glutamic acid 23 of t4 endonuclease v in the beta-elimination reaction of an abasic site in a synthetic duplex dna. | t4 endonuclease v catalyzes the hydrolysis of the glycosyl bond of a thymine dimer in a dna duplex and the cleavage of the 3'-phosphate by beta-elimination. we have previously identified a catalytic site for the first reaction (pyrimidine dimer-glycosylase activity) by systematic mutagenesis (doi et al. proc. natl. acad. sci. usa 1992 in press) and by x-ray crystallography (morikawa et al. science, 256: 523-526, 1992). the results showed that replacement of glu23 with either glutamine or asparti ... | 1992 | 1357629 |
| the functional activity of fc gamma rii and fc gamma riii on subsets of human lymphocytes. | subsets of human lymphocytes were isolated from peripheral blood using magnetic beads coated with anti-cd4, -cd8, -cd19 or -cd56 antibodies to yield t4, t8, b and natural killer (nk) cell suspensions with greater than 95% purity. the functional activity of fc gamma receptor ii (fc gamma rii) and fc gamma receptor iii (fc gamma riii) on these subsets was assessed by measuring rosette formation with red cells sensitized with known levels of either rabbit igg or human (monoclonal or polyclonal) igg ... | 1992 | 1356095 |
| hiv-associated oral lesions; immunologic, virologic and salivary parameters. | there are numerous reports of oral lesions in hiv-infected individuals. however, few correlate the oral lesions with laboratory parameters. this study examined oral candidiasis and hairy leukoplakia, the two most common hiv-associated oral lesions, in relation to t-cell counts, p24 core antigen levels and salivary flow rates. oral mucosal examinations, immunologic and virologic studies and stimulated whole and parotid saliva flow rates were conducted on 135 (hiv+ = 102, hiv- = 33) homosexual or ... | 1992 | 1355794 |
| life stressors and coping style are associated with immune measures in hiv-1 infection--a preliminary report. | life stressors and coping style have been associated with alterations in cellular immunity similar to those seen in hiv-1 infection. the interval between infection with hiv-1 and the development of aids is lengthy and highly variable. this pilot study investigated whether life stressors and coping style may account for a portion of this variation. | 1992 | 1355469 |
| diethylnitrosamine-induced increase in gamma-glutamyltranspeptidase in rat liver: its association with thyroid hormone deficiency and its reversal by tri-iodothyronine. | 1. the nodular phase of hepatic premalignancy was induced in male fischer 344 rats by the administration of diethylnitrosamine, 200 mg/kg i.p., followed by promotion utilizing the solt-farber promoting regime. 2. relative to the situation in normal non-treated control rats: the activity of gamma-glutamyltranspeptidase was found to be increased 9.42-fold in homogenate and 7.33-fold in plasma membrane fractions prepared from the livers of saline-injected control rats; and 81.37-fold in homogenates ... | 1992 | 1355453 |
| novel thiosemicarbazones derived from formyl- and acyldiazines: synthesis, effects on cell proliferation, and synergism with antiviral agents. | the synthesis of a series of novel thiosemicarbazones (tsc's) derived from various alkyl diazinyl (3-pyridazinyl, 4-pyrimidinyl, 2-pyrazinyl) ketones and 3-pyridazinecarbaldehyde and their evaluation against herpes simplex virus (hsv) and human immunodeficiency virus (hiv) as well as the determination of their cytotoxicity are described. in addition, the effects of combination of such tsc's with the well-known antiviral drugs acyclovir (acv) and 3'-azido-3'-deoxythymidine (azt) were studied. und ... | 1992 | 1354751 |
| [thyroid function tests in acute drug intoxication]. | it is well known that thyroid function tests may be changed in non-thyroidal illnesses. to understand the influence of acute drug intoxication on thyroid function tests, 31 drug intoxicated patients without previous thyroid disorders and systemic diseases were included in our study. t3, t4, tsh, and resin t3 uptake were checked as soon as they arrived at our emergency service and were compared to that of 58 healthy volunteers. within 31 patients, 14 were intoxicated by organophosphorous compound ... | 1992 | 1354715 |
| seasonal temperature and its influence on plasma corticosterone, triiodothyronine, thyroxine, plasma protein and packed cell volume in mature male chickens. | 1. the relationship between seasonal changes in environmental temperature and hematological parameters was investigated in mature, single comb white leghorn (scwl) male chickens. 2. samples of blood plasma, obtained monthly from two groups of birds over two separate 12 month periods, were analysed for corticosterone (ct), 3,5,3'-triiodothyronine (t3), thyroxine (t4), plasma protein (pp), and packed cell volume (pcv). 3. statistical analyses revealed that blood plasma concentrations of t3 were si ... | 1992 | 1354579 |
| essential catalytic role of glu134 in endo-beta-1,3-1,4-d-glucan 4-glucanohydrolase from b. licheniformis as determined by site-directed mutagenesis. | site-directed mutagenesis experiments designed to identify the active site of bacillus licheniformis endo-beta-1,3-1,4-d-glucan 4-glucanohydrolase (beta-glucanase) have been performed. putative catalytic residues were chosen on the basis of sequence similarity analysis to viral and eukaryotic lysozymes. four mutant enzymes were expressed and purified from recombinant e. coli and their kinetics analysed with barley beta-glucan. replacement of glu134 by gln produced a mutant (e134q) that retains l ... | 1992 | 1354172 |
| otitis media in children born to human immunodeficiency virus-infected mothers. | acute otitis media (aom) is thought to occur frequently in children infected with human immunodeficiency virus (hiv). we compared experience with aom of 28 hiv-infected children with that of 33 children who seroreverted to hiv antibody negative status by age 18 months. the mean number of episodes/year of aom for children who seroreverted decreased from 1.33 in the first year of life to 0.13 in the third year, whereas the mean number of episodes/year in hiv-infected children increased from 1.89 t ... | 1992 | 1352871 |
| optimized centrifugation for rapid elution of dna from agarose gels. | a simple method has been developed for the isolation of dna from agarose gels. centrifugation in a low-cost device for 45 s at low speed provides a high yield of dna suitable for further manipulation by restriction enzymes, t4 dna ligase, taq polymerase, klenow fragment, and t4 polynucleotide kinase, and also for sequencing. in contrast, centrifugation for greater than 1 min leads to coelution of substances that inhibit dna ligase. | 1992 | 1352695 |
| [differentiation of fetal lymphocytes with the dp-(dipeptidylpeptidase-) iv technique]. | the knowledge about the ontogenicity of the human immune system based on animal experiments. in this study expulsed fetus of women with sponeous and arteficial abortions were analysed because of the content of t4(+)-lymphocytes in the lymphatic tissues. the thymus, spleen, and a piece of the bone marrow were dissected, homogenised and the lymphocytes were separated. the identification of the t4+ cells was performed by the cytochemical staining of dideptidyl peptidase iv (dp iv). it could establi ... | 1992 | 1352420 |
| lymphokinetic monitoring of a liver transplant recipient treated with okt3. | orthotopic liver transplantation was performed on a 48-year-old female patient with end-stage liver disease due to primary biliary cirrhosis. a course of okt3 (5 mg/kg intravenously for 14 days) was given as immunosuppressive therapy. lymphocyte population, t lymphocyte subsets and the lymphokine level in the peripheral blood were serially measured. total t cells disappeared in the peripheral circulation within 10 minutes after an injection of okt3. helper t cells and suppressor t cells were als ... | 1992 | 1352344 |
| t lymphocyte changes in open heart surgery. | to investigate the effect of open heart surgery on t lymphocytes and their subpopulations, 20 patients, who had undergone moderate- to high-dose fentanyl anesthesia and a cardiopulmonary bypass (cpb), were studied using flow cytometry techniques and monoclonal antibodies during and after surgery. the ages of these patients ranged from four to 61 years with eight being male and 12 being female. the disease entity consisted of four with coronary, six with congenital and 10 with valvular heart dise ... | 1992 | 1352332 |
| studies of the cloned 37-kda subunit of activator 1 (replication factor c) of hela cells. | the elongation of primed dna templates by dna polymerase delta and dna polymerase epsilon requires the action of two accessory proteins, proliferating cell nuclear antigen and activator 1 (a1, also called replication factor c). a1 is an enzyme that contains five different subunits (145, 40, 38, 37, and 36.5 kda). in this paper, we describe the isolation of the gene encoding the 37-kda subunit from hela cells. this gene was cloned, sequenced, and overexpressed in escherichia coli. the amino acid ... | 1992 | 1351677 |
| stimulation of hepatic thyroxine 5'-deiodinase activity in rainbow trout (oncorhynchus mykiss) by pacific salmon growth hormone. | 1. growth hormone extracted from pacific salmon pituitaries (sgh) was injected intra-peritoneally into rainbow trout to determine sgh effects on plasma levels of thyroxine (t4), 3,5,3'-triiodothyronine (t3) and properties of the hepatic 5'-deiodinase enzyme (5'-d) responsible for t4 to t3 conversion. 2. after 24 hr, sgh (0.1 or 0.5 microgram/g) did not alter the plasma t4 level or 5'-d, km, but elevated plasma t3 and especially 5'-d vmax. 3. thus sgh, like the previously tested human gh, acutely ... | 1992 | 1351442 |
| the relationship of hiv-1 viral sequences detected by the polymerase chain reaction in haemophilic patients to clinical and other markers of infection. | a nested primer polymerase chain reaction (pcr) of pol gene sequences of human immunodeficiency virus-1 (hiv-1) was applied to whole blood of 31 haemophiliacs who were, or had been, positive for hiv p24 antibody (hivab) by enzyme linked immunosorbent assay (elisa) and samples from 22 persistently hivab negative haemophiliacs who had been at risk of contracting hiv from treatment. the results were compared with those of p24 hiv antigen determination, t4 cell counts beta 2 microglobulin (beta 2m) ... | 1992 | 1350951 |
| [effects of thyroxine and methimazolum on monamine transmitters in preoptic/anterior hypothalamic area and peripheral serum in rats]. | the contents of monamine transmitters in preoptic/anterior hypothalamic (po/ah) area and in the serum of rats injected with thyroxine (t4, 1 mg/100 g body wt/d, for 10 d, hypodermic i.) and fed with methimazolum (10 mg/100 g body wt/d, for 15 d) were assayed by high pressure liquid chromatography with electrochemical detector (hplc-ecd). it was found that the content of dopamine and homovanillic acid in po/ah area showed significant increase (p less than 0.01) associated with a slight rise (p gr ... | 1992 | 1350866 |
| lymphocytotoxic strains of feline leukemia virus induce apoptosis in feline t4-thymic lymphoma cells. | feline leukemia retrovirus (felv) strains with subgroup c env genes kill feline t4 lymphoma 3201 cells by 7 to 12 days after in vitro inoculation, whereas felv strains with subgroup a env genes do not. neither felv-a nor felv-c kill feline fibroblasts. felv-c, but not felv-a, is replicated to higher titer by 3201 cells and productive infection precedes death by 3 to 7 days. transcriptional activity of the felv-c long terminal repeat, as assessed by chloramphenicol acetyltransferase activity, is ... | 1992 | 1349933 |
| three-dimensional structure of the beta subunit of e. coli dna polymerase iii holoenzyme: a sliding dna clamp. | the crystal structure of the beta subunit (processivity factor) of dna polymerase iii holoenzyme has been determined at 2.5 a resolution. a dimer of the beta subunit (m(r) = 2 x 40.6 kd, 2 x 366 amino acid residues) forms a ring-shaped structure lined by 12 alpha helices that can encircle duplex dna. the structure is highly symmetrical, with each monomer containing three domains of identical topology. the charge distribution and orientation of the helices indicate that the molecule functions by ... | 1992 | 1349852 |
| aids--an autoimmune model. | in aids a complementary interface between the hiv virus and the cd4 molecule of the t4 lymphocyte suggest a possible cause of immune self-recognition. because of this complementarity, an anti-idiotypic immune response to the cd4 attachment area of hiv should result in an autoimmune reaction to cd4 positive lymphocytes. experimental demonstration of such an immune recognition model by autoreactive lymphocytes is presented and a hypothetical immune response unit is suggested. | 1992 | 1349151 |
| the replication of dna containing the simian virus 40 origin by the monopolymerase and dipolymerase systems. | the influence of dna polymerase (pol) alpha and dna primase on sv40 dna replication was examined in both the monopolymerase and dipolymerase systems. the synthesis of oligoribonucleotides in the monopolymerase and dipolymerase systems, followed by pulse labeling with deoxynucleoside triphosphates, yielded short okazaki fragments approximately 35 nucleotides in length that were chased into full-length okazaki fragments with time. in the presence of activator 1 and proliferating cell nuclear antig ... | 1992 | 1348504 |
| identification of dna-replicating lymphocyte subsets using a new method to label the bromo-deoxyuridine incorporated into the dna. | a flow cytometric procedure measuring the 5-bromo-2-deoxyuridine incorporated into the dna of cells in s phase was modified to make it compatible with the immunofluorescence labelling of cell surface antigens. the modifications were introduced at the stages of cell fixation and dna denaturation. ethanol was replaced by tween 20-containing paraformaldehyde and hydrochloric acid was used for the denaturation of dna by bovine pancreatic dnase-i. these two modifications permitted the preservation of ... | 1992 | 1347775 |
| aerobic fitness and hormonal responses to prolonged sleep deprivation and sustained mental work. | this study examined the influence of aerobic fitness on the responses of selected hormones to the combined stressors of sleep deprivation (sd) and sustained mental work. six aerobically high fit (hf) (vo2max greater than 50 ml.kg-1.min-1) and six average fit (af) (vo2max less than 40 ml.kg-1.min-1) female subjects were subjected to a period of sleep loss of 60 h during which time they performed sustained mental tasks with no physical activity component. venous blood samples were drawn every 12 h ... | 1992 | 1347679 |
| t4+ cell numbers are correlated with plasma glutamate and cystine levels: association of hyperglutamataemia with immunodeficiency in diseases with different aetiologies. | human immunodeficiency virus type 1 (hiv-1) seropositive individuals suffer from a depletion of t4+ t cells and have elevated plasma glutamate levels. glutamate is also elevated in cancer patients, and several authors have shown that elevated extracellular glutamate levels inhibit competitively the membrane transport of cystine and cause a decrease of intracellular cystine. we, therefore, tested the hypothesis that high glutamate and/or low cystine levels may generally be associated with low lym ... | 1992 | 1347232 |
| hiv-1 in blood monocytes: frequency of detection of proviral dna using pcr and comparison with the total cd4 count. | in vivo infection of monocytes/macrophages by the human immunodeficiency virus (hiv) has been investigated in many studies since these cells were suggested to provide a reservoir for the virus. in this study, we wanted to find out whether hiv provirus could be detected in circulating monocytes and whether it could be compared with the provirus found in t lymphocytes (t-ly). twenty-one seropositive subjects were studied. the amplification method (pcr) was used with three different primer pairs (i ... | 1992 | 1347227 |
| a mechanism of immune escape by slow-replicating hiv strains. | strains of human immunodeficiency virus (hiv) differ greatly in their ability to replicate in t4 cells. fast-replicating strains are observed during the early and late stages of hiv infection, while slow-replicating strains prevail during the intermediate, latent, stage. the prevalence of slow-replicating strains has been attributed to these strains' ability to escape the immune response. however, how these strains are able to avoid being eliminated by an immune response for several years has no ... | 1992 | 1346634 |
| effects of interferon-alpha on human b cell responsiveness: biphasic effects in cultures stimulated with staphylococcus aureus. | although interferon-alpha (ifn-alpha) has been found to be involved in the immune regulation in vivo, the effects of ifn-alpha on human b cells have not yet been clarified because of conflicting results in the literature. the present study therefore examined the effects of several subtypes of ifn-alpha (natural, alpha 1, alpha 2a, alpha 2b) on b cell responsiveness in detail by comparing different experimental conditions. highly purified b cells from normal human individuals were cultured with s ... | 1992 | 1346369 |
| tnf-alpha production by u937 promonocytes is enhanced by factors released from hiv-infected t4 lymphocytes: tnf-alpha is one of the mediators causing lysis of hiv-infected t4 cells. | in the present study, we have shown that the addition of culture supernatants from hiv-infected supt1 cells (t4) but not from noninfected cells markedly increased the production of tnf-alpha by u937 promonocytic cells after stimulation with phorbol 12-myristate 13-acetate (pma). pretreatment of supernatants with the antibodies to granulocyte/macrophage colony-stimulating factor (gm-csf) or tnf-alpha, but not interferon-gamma, significantly diminished this enhancing effect. these results suggest ... | 1992 | 1346100 |
| identification of replication factor c from saccharomyces cerevisiae: a component of the leading-strand dna replication complex. | a number of proteins have been isolated from human cells on the basis of their ability to support dna replication in vitro of the simian virus 40 (sv40) origin of dna replication. one such protein, replication factor c (rfc), functions with the proliferating cell nuclear antigen (pcna), replication protein a (rpa), and dna polymerase delta to synthesize the leading strand at a replication fork. to determine whether these proteins perform similar roles during replication of dna from origins in ce ... | 1992 | 1346062 |
| [free myocutaneous flap of the latissimus dorsi in cervicofacial surgery]. | free myocutaneous latissimus dorsi transplants are exceptionally used in cervicofacial surgery. the authors have performed an anatomical study of 23 non-embalmed subjects, using injections of neoprene latex and barium sulfate into the axillary artery and dye injections. dissections, arteriographs and corrosion show that the lower scapular pedicle is constant, with an average length of 9.5 cm and a caliber that is sufficient for vasuclar microsurgery. eight patients with large t4 cervicocephalic ... | 1992 | 1345697 |
| [goiter in school children from the swietokrzyski region]. | 5982 of children living in kielce district, aged 6.5-14.5 years were randomly selected for the study. the size of the thyroid gland was examined clinically. in some children the estimation of serum t3, t4 and tsh was performed, as well as iodine urine excretion. the iodine content in drinking water was also measure. the results of the study show that the kielce district belongs to the mild degree of iodine deficiency. the verification of the iodine prophylaxis in this region is necessary. | 1992 | 1345586 |
| [prevalence of goiter in children of the poznan province]. | the aim of our study was the evaluation of goiter prevalence in the children in poznań and surrounding towns and villages. in 3065 children 4-16 years old the thyroid size and serum concentration of t3, t4 and tsh were determined. our results indicate that the goiter prevalence in the group of investigated children was 20%. we also observed that the changes in the concentration of thyroid hormones and tsh were not associated with the clinical symptoms of thyroid gland dysfunction. | 1992 | 1345581 |
| [changes in laboratory parameters of thyroid function in children with simple goiter from the mazovia region]. | endocrinological out-patients clinic at children's hospital in dziekanów leśny takes care of the children from mazovia region. hormonal findings in children with simple goiter were analyzed in several groups of patients. in all those patients preferential t3-secretion and decreased t4 production was found. these findings allow to conclude, that in mazovia region there is iodine deficiency, which is probably the main cause of goiter development. | 1992 | 1345580 |
| [treatment of hypothyroidism with l-thyroxin]. | to compare an efficacy of the galenic form of desiccated thyroid gland--thyreoideum "polfa" with the synthetic l-thyroxine (eltroxin glaxo) in the treatment of hypothyroidism 15 patients were investigated. in all 15 cases before and after treatment ecg and the serum concentrations of cholesterol, thyroxine (t4), triiodothyronine (t3) as well as thyrotropin (tsh) in response to trh were performed. after the treatment with thyreoideum "polfa" in doses 0.2 to 0.6 mg/daily there were neither clinica ... | 1992 | 1345569 |
| [relationship between clinical classification of hyperthyroidism for three grades of severity and level of triiodothyronine (t3) and thyroxine (t4) concentrations in serum]. | the aim of the study was to assess the relationship between the clinical classification of hyperthyroidism based on the 3-degree score system and t3 and t4 serum concentration. 161 patients with graves disease or toxic goiter were studied. by comparing the number of scores separating the 3 subgroups in relation to the severity of disease with t3 and t4 serum concentration of tyreotoxic patients we found a very high statistically significant correlation. we also found the marked (by +50%) statist ... | 1992 | 1345568 |
| [the effect of monotherapy on concentration of selected blood serum hormones and upon cognitive function of children with epilepsy]. | the studies included 64 children with newly diagnosed epilepsy, aged from 6 to 15 years of life. in 25 children with partial and secondary generalized seizures monotherapy with carbamazepine was introduced; in 19 children with primary generalized seizures--with phenobarbital, and in patients with both types of seizures--with primidone. monotherapy was controlled by means of blood serum drug concentration level monitoring; the therapy was successful in all the children. the group did not include ... | 1992 | 1345559 |
| [thyroid function in patients during long-term treatment with amiodarone]. | thyroid function was evaluated in 31 patients with disturbances of heart rhythm both before and after 13 months of treatment with amiodarone. an increase in blood serum t4 concentration and transient increase (only during the first three months of treatment) in tsh concentration have been observed in almost all the patients studied. clinically evident form of hyperthyroidism was found only in one female patient. in two patients the changes in hormone concentrations suggesting hyperthyroidism wer ... | 1992 | 1345524 |
| [monitoring of treatment for hypothyroidism with l-thyroxine]. | the study was aimed at the evaluation of treatment of hypothyroidism with l-thyroxine administration monitored by the determination of t3 and t4 concentrations. the investigations were carried out in a group of 57 patients with hypothyroidism including 37 patients with autoimmune etiology of hypothyroidism, 12 patients after strumectomy and 8 patients after treatment with 131j. the administration of l-thyroxine at a dose of 2 micrograms/kg/day effectively eradicated all symptoms of the disease a ... | 1992 | 1345365 |
| [epidemiology and prognosis of cervix cancer. experience of the university gynecology and obstetric clinic ignace deen of conakry in 10 years: 1982-1991]. | the cancer of the cervix is a tumor that can be detected at an early stage. unfortunately, it is still detected lately in our service. thus, it is the underlying cause of death by gynecological affection. after a preliminary work in 1982 about 35 case histories, the authors undertake a 10 years retrospective study where they showed that: the cancer of the cervix is the primary gynecological cancer (78.7%), it obtains between 25 and 65 years, the most often associated factors are early sex, (28%) ... | 1992 | 1345096 |
| fine needle aspiration biopsy--a critical investigation in thyrotoxicosis. | four hundred and fifty two patients having clinical features of thyrotoxicosis have been studied for their hormonal (t4, t3 and tsh) content, i131 uptake levels and fnab at repeated intervals. four hundred and twenty seven had presented with diffuse enlargement and rest 25 cases with nodular enlargements. of the primary hyperthyroidism cases 342 (82.4%) were of grave's disease without exophthalmos and 73 (17.6%) with exophthalmos. t4, t3 and i131 uptake levels have correlated well with the degre ... | 1992 | 1344156 |
| [the dynamics of the hormonal and tumor marker levels in response to adrenaline administration in lung cancer patients]. | cia, ferritin, acth, cortisol, tth, t3, t4, insulin, ct and pth levels were assayed radioimmunologically in the blood serum of 227 patients with lung cancer, stages i-iv, 134 cases of chronic nonspecific diseases of the lung, 28 patients with benign tumors of the lung and 30 healthy subjects. adrenaline tests were carried out in 160 of them. similar shifts were observed in hormone profile in both cancer and non-cancer patients. the predictive value of the hormone tests for stage i-ii cancer appe ... | 1992 | 1343147 |
| contribution of a posturographic six-test set to the evaluation of patients with peripheral vestibular disorders. | a posturographic six-test set was developed in order to investigate the contribution of each sensory input in the balance performance of patients with peripheral vestibular disturbances. in test 1 (t1) the three sensory inputs are contributing to balance. in (t2), the contribution of vision is excluded by closing the eyes; in (t3) the neck proprioceptor is influenced by extreme retroflexion of the head, and in (t4) the input of the foot-ankle proprioceptor is altered by standing on foam rubber. ... | 1992 | 1342390 |
| zinc affects the metabolism of thyroid hormones in children with down's syndrome: normalization of thyroid stimulating hormone and of reversal triiodothyronine plasmic levels by dietary zinc supplementation. | levels of circulating thyroid stimulating hormone (tsh), tetraiodothyronine (t4), 3,5,3'-triiodothyronine (t3), and 3,3',5' triiodothyronine (reversal t3 or rt3) were measured in 25 children with trisomy of chromosome 21, also known as down's syndrome (ds), and in 14 normal children. in subjects with ds tsh levels were increased, while plasmic levels of rt3 were decreased. no alteration in t3 and t4 levels was observed. before zinc supplementation, plasmic levels of zinc and thymulin, a zinc dep ... | 1992 | 1341688 |
| is the new uicc staging system of gastric cancer reasonable? (comparison of 5-year survival rate of gastric cancer by old and new uicc stage classification). | in the new uicc tnm system for gastric cancer approved in 1985, a t1 lesion with lymph node (ln) metastasis is classified as stage, ib; in the old tnm system this was classified as stage iii. this is contradictory to a general rule of the uicc tnm system, whereby cancer with ln metastasis is classified as stage ii or iii. two thousand and sixty-three patients with less than t4 gastric cancer who were treated at seoul national university hospital from 1970 to 1986 were analysed for significant pr ... | 1992 | 1341253 |
| [hypocalcemia causing heart failure]. | a 39-year-old female patient with refractory heart failure has been studied. on february, 1982 she was submitted to right lobar thyroidectomy for remotion of the left thyroid lobe. following the surgery, she had signs of hypocalcemia and the diagnosis of secondary hypoparathyroidism and heart failure had been made. seven months after she had acute pulmonary edema, cardiomegaly iii (cardiothoracic index = 0.58) with predominant left atrial and left ventricular hypertrophy, which were confirmed by ... | 1992 | 1340740 |
| [effects of various quantities of dietary iodine on total serum t3 and t4 in gilts during various phases of the reproductive cycle]. | the effect of different dietary iodine levels (1 p.p.m.-control, 2 p.p.m. and 3 p.p.m.) on the serum levels of thyroid hormones (t3 and t4) has been studied in gilts at various stages of reproductive cycle, as well as on the their reproductive performances. the gilts were the crossbred of swedish landrace x big yorkshire x german landrace, selected for reproduction. the serum concentrations of t3 and t4 in four month old gilts (at the beginning of experiment) were normal: 1.48 +/- 0.28 and 46.57 ... | 1992 | 1340479 |
| [theory of multistep lymphocyte transformation]. | the transformation of b lymphocytes in lymph nodes proceeds from the specialized small (centrocytoid) blast cell of the lymphatic tissue germinal centre base. it runs three subsequent steps. the transformation stream of lymphocytes of the first step is derived from the basal matrix of germinal centres and is aimed towards its replica in the mantle zone. therein the transformed cells are supplemented and qualitatively altered towards the prevailing monocytic cells of the second step. the monocyto ... | 1992 | 1340397 |
| changes in the plasma levels and basic pharmacokinetic parameters of digoxin used in combination with gentamicin, amiodarone and spironolactone. | digoxin was administered intravenously (0.035 mg/kg b.w.). a prior five-day treatment with gentamicin (100 mg/kg b.w., 10 mg/kg b.w., i.m.), amiodarone (30 mg/kg b.w., s.c.) and spironolactone (10 mg/kg b.w., p.o.) caused elevation of plasma digoxin levels primarily because the alpha half-life was prolonged (t1/2 alpha). in a combined five-day application of digoxin with gentamicin (10 mg/kg b.w., i.m.), and of digoxin with amiodarone, the plasma levels of digoxin rose due to the lengthened beta ... | 1992 | 1339769 |
| a retrospective study on the treatment and evolution of graves' ophthalmopathy. | although graves' ophthalmopathy (go) seems to be unanimously considered as an autoimmune disease, its pathogenesis is still unknown. that is why the different therapeutical formulas led to ambiguous results. we think that a critical retrospective analysis on our therapeutical possibilities in go will help us to become aware of our limits in treating this pathology. our study performed on 123 patients with go-stage iii-iv who were admitted several times in the thyroid department of our institute ... | 1992 | 1339521 |
| chronobiology of pituitary-thyroid functions. | one hundred ninety four children, 11 +/- 1.5 years of age and 166 elderly men and women, 77 +/- 8 years of age were studied over one or (in the case of some of the elderly subjects) over several (up to 4) 24-hours spans. all subjects were diurnally active and rested at night and followed their regular three meal pattern. the subjects were studied in subgroups of 20-25 during all four seasons of the year. during each study, blood was collected at 4 hour intervals over one 24-hour span (6 samples) ... | 1992 | 1339520 |
| ligation-independent cloning of glutathione s-transferase fusion genes for expression in escherichia coli. | a plasmid vector has been constructed that allows the ligation-independent cloning of cdnas in any reading frame and directs their synthesis in escherichia coli as glutathione s-transferase-linked fusion proteins. the cloning procedure does not require restriction enzyme digestion of the target sequence and does not introduce any additional sequences between the thrombin cleavage site and the foreign protein. extended single-stranded tails complementary between the vector and insert, generated b ... | 1992 | 1339364 |
| [diurnal variations in circulating leukocytes and subsets: relation to plasma cortisol and acth]. | surface cellular antigens of leukocytes, lymphocytes and corresponding subpopulations have been analysed by using monoclonal antibodies marked with fluorescein (pitc), parallel to those marked with phycoerythrin (pe). cortisol and acth plasmatics have also been determined through ria, on two samples at 8am. and 8pm. during this twelve hour evolution, a highly significant dependence of the leukocytes, t, t4 and t8 lymphocytes on the circulating acth has also been found. in general during the expe ... | 1992 | 1338882 |
| [serum activity of angiotensin-converting enzyme and osteocalcin levels in hyperthyroidism]. | this study was carried out in order to investigate serum changes of osteocalcin (bgp) and angiotensin converting enzyme (sace) activity in a group of patients with hyperthyroidism. we studied 20 hyperthyroid patients (f 14, m 6; age mean 37.5 +/- 16.8 years) and 13 control subjects (f 11, m 2; age mean 40.3 +/- 7.5 years). in both patients and controls we measured: ft3, ft4, t3, t4, tsh, bgp, sace. finally, in patients with hyperthyroidism a trh test and functional investigations were also perfo ... | 1992 | 1338548 |
| the alpha 1-adrenergic receptor in human erythrocyte membranes mediates interaction in vitro of epinephrine and thyroid hormone at the membrane ca(2+)-atpase. | membrane ca(2+)-atpase activity was stimulated in vitro separately by t4 (10(-10) m) and by epinephrine (10(-6) m). in the presence of a fixed concentration of t4, additions of 10(-8) and 10(-6) m epinephrine reduced the t4 effect on the enzyme. beta-adrenergic blockade with propranolol (10(-6) m) prevented stimulation by epinephrine of ca(2+)-atpase activity, but did not prevent the suppressive action of epinephrine on t4-stimulable ca(2+)-atpase. in contrast, alpha 1-adrenergic blockade with u ... | 1992 | 1336972 |
| [what is the role of thoracoscopy in the preoperative staging of non-small cell bronchial cancer?]. | it is of paramount importance to clearly separate the non small cell bronchogenic cancers involving the chest wall or the diaphragm (t3) and those metastasizing to the pleura (t4). in both events, surgery is either technically demanding or contraindicated. what is the contribution of thoracoscopy to diagnosis? very few authors have used thoracoscopy in a restricted number of cases and with varying results to confirm tumoral extent to the chest wall. we are of opinion that this approach is only w ... | 1992 | 1336868 |
| induction of topoisomerase ii-mediated dna cleavage by the plant naphthoquinones plumbagin and shikonin. | plumbagin and shikonin, plant metabolites which have naphthoquinone structures, induced mammalian topoisomerase ii-mediated dna cleavage in vitro. treatment of a reaction mixture containing these naphthoquinones and topoisomerase ii at an elevated temperature (65 degrees c) resulted in a great reduction in dna cleavage, suggesting that the mechanism of the topoisomerase ii-mediated dna cleavage induced by these naphthoquinones is through formation of a cleavable complex, as seen with antitumor a ... | 1992 | 1336338 |
| bound lac repressor protein differentially inhibits the unwinding reactions catalyzed by dna helicases. | a partial duplex dna substrate containing the lac repressor binding site, within the duplex region, was constructed to examine the effect of bound lac repressor on the unwinding reaction catalyzed by several dna helicases. the substrate contained 90 base pairs of double-stranded dna and, in the absence of lac repressor, was effectively unwound by each of the seven helicases tested. the unwinding reactions catalyzed by escherichia coli rep protein, bacteriophage t4 dda protein and e. coli dna hel ... | 1992 | 1336182 |
| electrophoresis, autoradiography and electroblotting of peptides: t4 gene 60 hopping. | 1992 | 1335733 | |
| [a case of chronic thyroiditis with transient painful thyroiditis occurring after the administration of lipiodol]. | we reported a female case of painful thyroiditis occurring after hysterosalpingography and investigated whether the destructive thyroiditis was iodine-induced. the patient, aged 28, had tbg deficiency and the previous episode of thyrotoxicosis caused by graves' disease. lipiodol (containing 4.8g of iodide in 10ml solution) was administered via vagina for hysterosalpingography. one month after the radiography, serum inorganic iodide and tg were elevated abnormally, but she was asymptomatic. after ... | 1992 | 1333996 |
| in vivo effects of the steroid analogue ru486 on some aspects of intermediary and thyroid metabolism of brook charr, salvelinus fontinalis. | the glucocorticoid analogue ru486 was administered by intraperitoneal injection to brook charr (salvelinus fontinalis) to further explore the role of cortisol on aspects of intermediary and thyroid hormone metabolism of the species. ru486 significantly elevated the hepatosomatic index, hepatic g3pdh activity, and hepatic glycogen content, but was without effect on hepatic protein content, hepatic fpbase activity, or plasma glucose concentration. however, the stressor-related increase in plasma g ... | 1992 | 1333515 |
| kinetic characterization of the polymerase and exonuclease activities of the gene 43 protein of bacteriophage t4. | the dna polymerase from the bacteriophage t4 is part of a multienzyme complex required for the synthesis of dna. as a first step in understanding the contributions of individual proteins to the dynamic properties of the complex, e.g., turnover, processivity, and fidelity of replication, the minimal kinetic schemes for the polymerase and exonuclease activities of the gene 43 protein have been determined by pre-steady-state kinetic methods and fit by computer simulation. a dna primer/template (13/ ... | 1992 | 1332748 |
| atracurium-induced neuromuscular block in the isolated arm. | a modification of the isolated arm technique was applied in 10 females under opioid-based i.v. anaesthesia for comparison of the offset of an atracurium-induced neuromuscular block in an isolated arm to an arm with maintained circulation. the neuromuscular blocking effect of a bolus dose of atracurium 0.5 mg.kg-1 was measured bilaterally using the integrated adductor pollicis emg response (integrated t1 emg response in % of baseline value and t4/t1 ratio) after bilateral ulnar nerve train-of-fou ... | 1992 | 1332358 |
| [inclusion body myositis and neuromuscular diseases with rimmed vacuoles]. | a retrospective study of 40 patients with various neuromuscular disorders and more than 3 muscle fibers with rimmed vacuoles has been performed. two subgroups of patients were distinguished according to the presence or absence of inflammatory exudates. in the first group (14 patients), inflammatory exudates were observed and numerous fibers showed partial invasion. abnormal filamentous inclusions (16-18 nm in diameter) were found by electron microscopy in muscle fibers cytoplasm and/or nuclei. t ... | 1992 | 1332175 |
| [results of surgery of non-small cell primary bronchial cancers. retrospective analysis of 452 resections]. | data from 452 patients who underwent pulmonary resection for a non small cell bronchogenic carcinoma from 1980 to 1985 were analysed retrospectively. the operative mortality rate was 5.5%. mortality was significantly increased in patients who underwent enlarged resections for t4 tumors (20%; p < or = 0.05), and in patients who were 70 years and older (12.8%; p < or = 0.05). the overall 5-years survival rate was 32.7% at 5 years. prognosis in patients who were 70 years and older was similar to th ... | 1992 | 1332149 |
| thyroid functions in patients treated with interleukin-2 and lymphokine-activated killer cells. | treatment of malignant disease with interleukin-2 and lymphokine-activated killer cells activates autoreactive t lymphocytes, stimulates release of cytokines and induces expression of hla-class ii antigens by tumour cells. we studied eight patients with hepatocellular carcinoma treated with a total of 16 courses of recombinant human interleukin-2 and lymphokine-activated killer cells and observed them for features of autoimmune thyroid disease. during the course of treatment there were significa ... | 1992 | 1332102 |
| consequences of 6-thioguanine incorporation into dna on polymerase, ligase, and endonuclease reactions. | the incorporation of 6-thioguanine (s6g) in place of guanine proceeds readily in dna synthesis reactions catalyzed by mammalian and bacterial polymerases. this report summarizes the consequences of such incorporation studied to date. s6g was incorporated into one strand of a defined m13mp18 phage sequence in a (+)reaction catalyzed by the klenow fragment of escherichia coli dna polymerase i. after denaturation of the newly synthesized strand (containing s6g) and annealing with a reverse (-) 32p- ... | 1992 | 1331762 |
| iodine-induced subclinical hypothyroidism in euthyroid subjects with a previous episode of amiodarone-induced thyrotoxicosis. | amiodarone-induced thyrotoxicosis (ait) occurs most frequently in patients with underlying thyroid disease and is generally believed to be due to the iodine contamination of amiodarone and iodine released by the metabolism of the drug. we and others have suggested that the thyrotoxicosis may also be secondary to amiodarone-induced thyroiditis. to further determine the etiology of ait, we administered large doses of iodides [10 drops saturated solution of potassium iodide (sski) daily] to 10 euth ... | 1992 | 1331165 |
| enzymatic analysis of oligonucleotides containing cyclobutane pyrimidine photodimers with a cleaved intradimer phosphodiester linkage. | our recent studies indicate that enzymatic hydrolysis of the intradimer phosphodiester linkage constitutes an early reaction in processing uv light-induced cis-syn-cyclobutane pyrimidine dimers in cultured human fibroblasts. before characterizing the resultant modified dimer sites in cellular dna, it is necessary to establish experimental conditions that can distinguish backbone-nicked from intact dimers. we thus constructed a model substrate, i.e. p(dt) 10 <> p(dt)10 containing a dimer with a r ... | 1992 | 1331055 |
| a human dimorphism resulting from loss of an alu. | the molecular phylogeny of alu and other repeated sequences in the human genome provides clues to events during primate evolution. a subclass of human alu's has been previously identified as dimorphic insertions within members of the medium reiteration frequency (mer) class of repeats, reflecting the complicated sequence of insertion and radiation events leading to the current human genome structure. one dimorphic alu is located within a previously unidentified mer family member, in the first in ... | 1992 | 1330888 |
| [the effect of stress on the enzyme systems of the enterocyte microvilli in rat pups and the possible mechanism of the transmission of stress effects from dam to progeny]. | in suckling rats whose mothers had had an immobilisation stress or been injected hc and t4 at the last day of their pregnancy, the sucrase induction decreased in the small intestine, if the hc was injected on the 10th day of life. the hormone injection to 1-day suckling rats did not affect the induction. these effects seem to be exerted not by the direct permeation of hormone from mother to litter but to be mediated rather by a special factor "stressin". the latter is transferred from mother to ... | 1992 | 1330718 |
| interrelationship between estradiol and thyroxine on the release of thyrotropin and prolactin in ovariectomized-thyroidectomized rats. | the effects of estradiol and thyroxine (t4) on the spontaneous and thyrotropin-releasing hormone (trh)-stimulated release of thyroid-stimulating hormone (tsh) and prolactin (prl) in vitro from rat anterior pituitary glands (aps) were studied. rats were ovariectomized (ovx) and thyroidectomized (tx) following injection of either thyroxine (20 micrograms/kg, sc) or saline and either estradiol benzoate (eb, 0.4 micrograms/kg, sc) or peanut oil daily for 42 days. twenty hours after the last injectio ... | 1992 | 1330453 |
| surgical treatment for higher stage non-small cell lung cancer. | locally advanced lung cancer (stage iiia, iiib) in which the primary tumor is proximal (t3) or has invaded adjacent structures (t3) or organs (t4) or in which mediastinal lymph nodes are involved (n2, n3) worsens the prognosis significantly. however, in stage iiia (t3 or n2), when surgical treatment results in total removal of the primary tumor and involved lymph nodes, there still is a reasonable chance for ultimate cure. on the other hand, total excision can be very rarely performed in t4 or n ... | 1992 | 1329685 |
| acute development of low t3 syndrome and changes in pituitary-adrenocortical function after elective cholecystectomy in women: some differences between young and elderly patients. | from two groups of female patients (young group: 15 patients aged less than 25 years; elderly group: 15 patients aged more than 65 years) who were subjected to cholecystectomy, blood samples were obtained on the day before surgery, on the day of surgery before premedication and after waking up from anaesthesia and also on days 1, 3 and 7 after surgery. in aliquots of sera the levels of tsh, acth, thyroxine (t4), triiodothyronine (t3), reverse triiodothyronine (rt3) and cortisol were estimated wi ... | 1992 | 1329184 |
| early adaptation of thyrotropin and thyroglobulin secretion to experimentally decreased iodine supply in man. | five healthy male volunteers (aged 25 to 28 years) were studied both after 4 weeks of treatment with 200 micrograms iodine/d orally (po) and following experimental iodine depletion by treatment with 3 x 300 mg perchlorate/d po over a 4-week period, in an attempt to better define the early adaptive responses to an alteration in iodine supply in thyroid function. intrathyroidal iodine, serum triiodothyronine (t3), free t3 (ft3), thyroxine (t4), free t4 (ft4), reverse t3 (rt3), thyroxine-binding gl ... | 1992 | 1328820 |
| overexpression, purification, sequence analysis, and characterization of the t4 bacteriophage dda dna helicase. | the bacteriophage t4 dda protein is a 5'-3' dna helicase that stimulates dna replication and recombination reactions in vitro and seems to play a role in the initiation of t4 dna replication in vivo. oligonucleotide probes based on nh2-terminal amino acid sequence were used to precisely map the location of the dda gene on the t4 chromosome. using polymerase chain reaction techniques, the dda gene was then cloned into an expression vector, and the overproduced protein was purified in two chromato ... | 1992 | 1328208 |
| technique for the primary culture of human breast cancer cells and measurement of their prostaglandin secretion. | 1. a method is described for the primary culture of human breast tumour cells on feeder layers of the sto mouse embryo fibroblast cell line. 2. the secretion of the prostaglandins e2 and f2 alpha from the cells was measured and the results indicate that the secretion of both prostaglandins was dependent on oestrogen-receptor status, with cells from oestrogen-receptor-positive tumours secreting significantly more prostaglandin than cells from oestrogen-receptor-negative tumours. 3. prostaglandin ... | 1992 | 1327654 |
| dna polymerase, rna polymerase and exonuclease activities on a dna sequence modified by benzo[a]pyrene diolepoxide. | adducts produced by modification of dna with benzo[a]pyrene diolepoxide (bpde) are known to inhibit both dna and rna synthesis. this phenomenon has been used as a method for determining the distribution of carcinogen binding within defined dna sequences. a critical comparison of different enzyme activities on adducted dna is needed, since different enzymes may process adducted dna differently. thus, we compared blocks in dna polymerase activity with that of an rna polymerase and with an exonucle ... | 1992 | 1327570 |
| alterations in endothelium-associated proteins and serum thyroid hormone concentrations in anorexia nervosa. | plasma concentrations of endothelium-associated proteins (eap) (plasma fibronectin (pfn), angiotensin-converting enzyme, factor viii-related antigen (f viii-r:ag)) and tissue plasminogen activator and serum thyroid hormone concentrations were studied in nine patients with anorexia nervosa (an), before and after weight gain. before weight gain (-35.9 (se 2.3)% of standard body-weight) pfn was significantly reduced and f viii-r:ag was significantly increased in an patients compared with the concen ... | 1992 | 1327101 |
| relationship of the train-of-four ratio to plasma atracurium concentration. | the electromyographic response to a short infusion of atracurium 0.25 mg kg-1 was recorded using the train-of-four (tof) technique, and the plasma atracurium concentration profile measured, in 10 healthy patients. the tof ratio (t4:t1) was depressed over a time course which did not conform to the predictions of an effect compartment model; the fit of such a model, when possible, was associated with large residual errors. in contrast, the absolute height of the fourth response of the tof (t4:t0) ... | 1992 | 1327041 |
| two cellular single-strand-specific dna-binding proteins interact with two regions of the bovine papillomavirus type 1 genome, including the origin of dna replication. | we have identified and purified to near homogeneity two specific single-stranded dna-binding factors (spsf i and ii) with molecular masses of 42 and 39 kda, respectively, from calf thymus. gel retention analysis and competition experiments demonstrate that the ubiquitous proteins spsf i and ii specifically interact with single-stranded dna derived from the minimal in vitro origin of replication of bovine papillomavirus type 1 and a region of the viral genome proposed to be involved in plasmid ma ... | 1992 | 1326653 |
| [ulcerated breast cancer of the young patient: a case report]. | a 29-year-old woman presented with an exulcerating cancer of the right breast. she underwent a total mastectomy and axillary clearance which revealed a pathological stage of t4 n+ (21/23) m0. she received chemotherapy followed by radiation therapy and by chemotherapy again. 30 months later, a contralateral breast cancer was diagnosed. a close team-work of gynaecologist, plastic surgeon, medical and radiation oncologists allowed to combine the operation with the reconstruction of both breasts tog ... | 1992 | 1326503 |
| [radiotherapy of the maxillary sinus cancers]. | between 1983 and 1988, 23 patients with cancer of maxillary sinus were treated with either postoperative radiotherapy or radiation alone at the radiation oncology department, veterans general hospital-taichung. there were 20 males and 3 females, 38 to 80 years of age with median age of 60 years. nineteen were of epidermoid type and 4 were of other histologic types. there were 11 cases of t3 and 12 cases of t4. only 1 case had neck lymph node metastasis. all patients had bony destruction shown in ... | 1992 | 1326388 |
| characterization of cytoplasmic t3 binding sites by adsorption to hydroxyapatite: effects of drug inhibitors of t3 and relationship to glutathione-s-transferases. | to facilitate studies of thyroid hormone (t3) binding to cytoplasmic proteins, we prepared monkey (m. fascicularis) liver cytosol (100,000g supernatant) and examined t3 binding using hydroxyapatite (hap) separation. hap adsorbs cytoplasmic and nuclear binding sites but not serum t4 binding proteins. cytosol was incubated with [125i]t3 for 30 min at 4 degrees c and separated by adding an equal volume of hap (15 g/100 ml). after a further incubation of 10 min, the hap pellet was washed three times ... | 1992 | 1326362 |