Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| preliminary results of concurrent cisplatin and radiation therapy in locally advanced bladder cancer. | between march 1981 and march 1990, 15 patients with locally advanced transitional cell carcinoma of the bladder were treated concurrently with cisplatin and radiotherapy. treatment comprised a radiation dose of 40-50 gy in 20-25 fractions over 4-5 weeks and intravenous infusion of cisplatin with hydration during days 1-5 and 22-26. the total scheduled dose of cisplatin was 200 mg. a complete response (cr) was seen in 3 patients (2 t2 tumours and 1 t3) and the other 12 were regarded as partial re ... | 1992 | 1450848 |
| mr imaging with stir technique and air insufflation for local staging of bladder neoplasms. | fifty-six patients with bladder carcinoma were studied with mr imaging for the assessment of the local stage, using the inversion recovery pulse sequence with fat suppression (stir) with air in the bladder. with this technique images of the inner and the outer parts of the bladder wall were obtained, showing high contrast between the latter and the tumor (tumor/muscle contrast 89.9%). four tumor stages were recognized: superficial neoplasms (tis, ta, and t1), partial wall infiltrating neoplasms ... | 1992 | 1449884 |
| thyrotoxicity of the chlorides of cadmium and mercury in rabbit. | exposure to heavy metals such as cadmium and mercury is of immediate environmental concern. the present study was aimed at establishing a direct relationship between heavy metal poisoning and thyroid dysfunction. cadmium and mercury treatment at ld50 levels resulted in severe thyrotoxicosis in the rabbit. within 24 h of intramuscular administration of cadmium chloride 15 mg.kg-1 body weight (bw) and mercury chloride 20 mg.kg-1 bw, thyroid peroxidase activity increased significantly over the cont ... | 1992 | 1449659 |
| construction of a broad host range shuttle vector for gene cloning and expression in actinobacillus pleuropneumoniae and other pasteurellaceae. | we have constructed a pair of broad host range expression vectors, pjff224-nx and pjff224-xn, based on plasmid rsf1010, which enable cloning and efficient expression of genes in actinobacillus pleuropneumoniae and pasteurella haemolytica and in escherichia coli. the vectors consist of the minimal autonomous replicon of the broad host range plasmid rsf1010 and a type ii chloramphenicol acetyl transferase gene for chloramphenicol resistance selection. in addition, they contain a gene expression ca ... | 1992 | 1448612 |
| role of mota transcription factor in bacteriophage t4 dna replication. | at least two bacteriophage t4 replication origins, ori(uvsy) and ori(34), contain a t4 middle-mode promoter that is necessary for origin function. we wanted to analyze the requirement of these two replication origins for the mota protein, which is the phage-encoded activator of middle-mode promoters. to ensure the complete absence of mota protein, we deleted the mota gene from the t4 genome. unexpectedly, the deletion mutant was not viable unless the mota protein was provided from a recombinant ... | 1992 | 1447797 |
| characterization of the nucleic acid-binding activities of the isolated amino-terminal head domain of the intermediate filament protein vimentin reveals its close relationship to the dna-binding regions of some prokaryotic single-stranded dna-binding proteins. | in order to demonstrate that the nucleic acid-binding activities of vimentin are dictated by its arg-rich n-terminal head domain, this was cut off at position lys96 with lysine-specific endoproteinase and analysed for its capacity to associate with a variety of synthetic and naturally occurring nucleic acids. the isolated polypeptide (vim nt) showed a preference for single-stranded (ss) polynucleotides, particularly for ssdnas of high g-content. a comparison of the sequence and predicted seconda ... | 1992 | 1447793 |
| serum concentrations of osteocalcin in patients with hyperthyroidism, hypothyroidism and subacute thyroiditis. | serum concentration of osteocalcin (oc) was measured in sera from untreated patients with graves' disease, hypothyroidism due to hashimoto's thyroiditis, and subacute thyroiditis. serum concentration of oc in graves' disease and hypothyroidism were 14.1 +/- 5.6 micrograms/l and 3.8 +/- 2.7 micrograms/l, respectively which were significantly different from that of healthy subjects (graves' disease, p less than 0.001, hypothyroidism, p less than 0.01). serum concentration of oc in patients with su ... | 1992 | 1447487 |
| impact of adjuvant therapy on locally advanced adenocarcinoma of the stomach. | from 1975 to 1988, 120 consecutive patients with locally advanced (t3/t4 or n1/n2) adenocarcinoma of the stomach underwent attempted curative resection. seventy patients were treated with surgery alone while 50 patients also received adjuvant therapy consisting of either chemotherapy (5-fu/fam) alone, radiation therapy alone, or chemotherapy+radiation therapy. adjuvant therapy was tolerated relatively well with only one patient experiencing a grade 3 (rtog/eortc) toxicity, and none experiencing ... | 1992 | 1447035 |
| purification and characterization of rat brain cytosolic 3,5,3'-triiodo-l-thyronine-binding protein. evidence for binding activity dependent on nadph, nadp and thioredoxin. | a rat brain cytosolic 3,5,3'-triiodo-l-thyronine-(t3)-binding protein (ctbp) was purified using, successively, carboxymethyl-sephadex, deae-spherodex, t3-sepharose-4b affinity chromatography and sephacryl s-200. the molecular mass determined by sds/page wa 58 kda. the binding characteristics determined by scatchard analysis revealed a single class of binding sites with a ka of 1.56 nm-1 and a maximal binding capacity of 7500 nmol t3/g protein. the relative binding affinities of iodothyronine ana ... | 1992 | 1446686 |
| detection of albumin binding abnormalities in sera of patients with familial dysalbuminaemic hyperthyroxinaemia using isoelectric focusing. | definitive diagnosis of familial dysalbuminaemic hyperthyroxinaemia (fdh) requires finding a high concentration of [125i]t4 bound to albumin. we used isoelectric focusing (ief) in agarose gels to study the sera of three members of a family with fdh and compared the distribution of [125i]t4 obtained by ief with that obtained by conventional agarose gel electrophoresis (age). both ief and age confirmed the diagnoses of fdh. in case #1, the % [125i]t4 bound to albumin was 15.2 and 20.0, with ief an ... | 1992 | 1446661 |
| testosterone regulates epidermal growth factor levels in the thyroid gland of hypothyroid mice. | the effects of testosterone (tp) and thyroxine (t4) on the level of epidermal growth factor (megf) in the thyroid were compared in a hypothyroid mouse model. groups of five adult female balb/c mice were given a "severe" hypothyroid regimen consisting of an iodine deficient diet together with oral and s.c propylthiouracil (ptu). sialoadenectomy or sham operation was performed after 18 days on the hypothyroid regimen. the mice convalesced on normal diet for 5 days and beginning from day 23 receive ... | 1992 | 1446659 |
| thyrotoxicosis with low serum total t3 level in patients with destructive thyroiditis and non-thyroidal illness. | the serum total t3 level, evaluated in 687 patients with thyrotoxicosis diagnosed by an elevated serum free t4 level and suppressed serum tsh level, was found to be high in 98.1% and normal in 1.9% of 592 patients with graves' hyperthyroidism, and high in 75.8%, normal in 21.1% and low in 3.2% of 95 patients with destructive thyroiditis. non-thyroidal illness was found in about a third of the patients with thyrotoxicosis and a normal serum total t3 level. the serum total t3 level was low with el ... | 1992 | 1446653 |
| thyroid hormone synthesis in thyroglobulin secreted by porcine thyroid cells cultured on porous bottom chambers. effect of iodide. | the long-term iodination of thyroglobulin secreted into the apical medium of thyroid cells cultured as monolayers on porous bottom chambers reached 5.87 +/- 1.66 atoms of iodine/mol thyroglobulin after 11 days incubation in the presence of tsh (0.1 mu/ml) and iodide (0.5 microm) in the basal medium. this iodinated thyroglobulin contained thyroid hormones (t3 + t4) which involved 22.7% of the thyroglobulin iodine content. the iodoamino acid content was, in residues per mole, 2.2 +/- 0.35 for mono ... | 1992 | 1446629 |
| thyroxine binding to the apolipoproteins of high density lipoproteins hdl2 and hdl3. | four preparations of high density lipoprotein hdl2, five of hdl3, and purified apolipoproteins apoa-i, apoa-iv, and apoe were photoaffinity labeled with [125i]t4 and analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. gels were also immunoblotted with antiserum against apoa-i, apoa-ii, apoa-iv, apoe, or apo(a), and the immunostained membrane was then autoradiographed. in hdl2, the two major radioactive bands migrated near the origin of the resolving gel and at 28-31 kilodalton ... | 1992 | 1446618 |
| central hypothyroidism is associated with advanced age in male fischer 344/n rats: in vivo and in vitro studies. | we investigated age-related alterations in hypothalamic-pituitary-thyroid function in a series of in vivo and in vitro studies in 2-, 8-, 18-, and 24-month-old male fischer 344/n (f344/n) rats. thyroid histology showed progressive follicular loss with advancing age; this was associated with significant and progressive decrements in plasma levels of free t4 and free t3, but not immunoreactive tsh, which remained unchanged with age. this was accompanied by a progressive age-dependent loss in in vi ... | 1992 | 1446609 |
| analysis of pulsatile secretion of thyrotropin and growth hormone in the hypothyroid rat. | to characterize the role of trh in the generation of tsh pulsatility as well as the effect of hypothyroidism on episodic gh secretion, blood was constantly withdrawn (30-60 microliters/min) from rats treated with 0.02% methimazole in the drinking water for 8-10 days. this treatment significantly reduced circulating levels of both t3 and t4 and elevated plasma tsh; however, since thyroid hormone titers were still detectable (t3, 39.6 +/- 5.3 vs. 89.8 +/- 5.3 ng/dl in euthyroid animals), methimazo ... | 1992 | 1446603 |
| variations of lymphocyte sub-populations in vulvar condylomata during therapy with beta-interferon. | several experiences induced us to consider genital hpv infection as an expression of a local immunodeficiency. the aim of our study was to research the effect of immunotherapy on the lymphocyte subpopulations and langerhans cells in vulvar condyloma. twenty women with persistent vulvar condylomata, treated with 2,000,000 iu/die of beta-interferon for 15 days, were submitted to vulvar biopsy before and 2-5 months after medical treatment. the frozen sections obtained were assayed with the followin ... | 1992 | 1446396 |
| the nucleotide sequence between genes 31 and 30 of bacteriophage t4. | the nucleotide sequence of the 2994 bp t4 phage dna fragment between genes 31 and 30 is presented. the fragment contains 7 complete open reading frames in the direction of early transcription and two early promoters, pe128.6 and pe128.2, which we show to cause difficulties in cloning dna from this genomic region. our data complete the nucleotide sequence and the organization of genes in the genomic region between t4 genes 31 and 30. | 1992 | 1446076 |
| [lymphocyte subpopulation state of the human lung during prenatal development]. | distribution of surface lymphocyte markers was assessed in foetus lung by cytofluorimetry using monoclonal antibodies produced by "ortho diagnostic systems" and "becton dickinson". seven lymphocyte subpopulations were detected in developing lungs even at the pseudoglandular stage (8-15 weeks). there was a significant increase in these lymphocyte subpopulations in canalicular stage. quantity of t3+ cells increased 8-fold and b-cells 10-fold. there was a tendency to increased number of cortical th ... | 1992 | 1446034 |
| tumour infiltrating lymphocytes as an independent prognostic factor in transitional cell bladder cancer. | the prognostic value of tumour infiltrating lymphocytes (til) was assessed in a cohort of 514 patients with a transitional cell bladder cancer (tcc) during a follow up period of over 9 years. the density of til were positively correlated to who grade (p < 0.0001), non-papillary growth architecture (p < 0.0001), morphometric nuclear factors (p < 0.007) and volume corrected mitotic index (m/v index) (p < 0.0001). dense til predicted progression in ta-t1 tumours (p < 0.0006) whereas in a multivaria ... | 1992 | 1445749 |
| a pilot study of intensive cyclophosphamide, epirubicin and fluorouracil in patients with axillary node positive or locally advanced breast cancer. | a multicentre pilot study has been conducted to determine an intensive regimen of cyclophosphamide, epirubicin, and fluorouracil which was tolerable and acceptable to patients with node positive breast cancer. consecutive patients with operable axillary node positive breast cancer (t1-3, n1-2, m0), 266 patients, or locally advanced breast cancer (t4), 22 patients, were treated with cyclophosphamide post-operatively for 14 days and epirubicin and fluorouracil, both intravenously on days 1 and 8. ... | 1992 | 1445744 |
| serum levels of fetal antigen 2 in hyperthyroidism and primary hyperparathyroidism. | serum concentration of fetal antigen 2 (fa2) in patients with hyperthyroidism (n = 18) (median: 12.9 mau/l; range: 3.2-22.4 mau/l) was significantly (p < 0.002) higher than in age- and sex-matched healthy controls (median: 4.1 mau fa2/l; range: 2.4-10.0 mau fa2/l). serum fa2 was positively correlated with thyroxine (t4) (rs = 0.51; p < 0.05), triiodothyronine (t3) (rs = 0.64; p < 0.01), bone-gla protein (bgp) (rs = 0.70; p < 0.01), total alkaline phosphatase (total-ap) (rs = 0.62; p < 0.01), bon ... | 1992 | 1445696 |
| direct electrochemistry of thioredoxins and glutathione at a lipid bilayer-modified electrode. | by using direct electrochemical analysis we have established that the reduction of escherichia coli thioredoxin (ect), t4 thioredoxin (t4t), and glutathione (gssg) occurs at a self-assembled lipid bilayer-modified gold electrode via two separate one-electron processes. the first electron transfer has half-wave potentials of -0.05 +/- 0.01, -0.07 +/- 0.01, and -0.06 +/- 0.01 v, whereas the second one has values of -0.48 +/- 0.01, -0.39 +/- 0.01, and -0.45 +/- 0.01 v, for ect, t4t, and gssg, respe ... | 1992 | 1444449 |
| investigation of the use of impermeable fluid barriers between pelleted and supernatant enzyme activity in a pseudohomogeneous enzyme immunoassay. | we have investigated the feasibility of performing an automated pseudohomogeneous enzyme immunoassay in which a separation step is not apparent to the user. this was achieved using a layer of silicone fluid, which is immiscible with aqueous solutions, as a physical barrier between the pelleted and supernatant enzyme. initially we produced a manual assay based on the serozyme t4 assay to demonstrate the feasibility of the approach. this assay showed good precision, parallelism and correlated with ... | 1992 | 1444167 |
| synthesis and properties of n-bromoacetyl-l-thyroxine. | a one-step bromoacetylation of l-thyroxine (t4) produces n-bromoacetyl-l-thyroxine (bract4) in good yield. the reaction product is best purified by high-speed countercurrent chromatography. while hplc is satisfactory only for purification of microgram and submicrogram quantities, amounts ranging from about 1 ng to 1 g of bract4 can be processed by high-speed countercurrent chromatography (hsccc), a method which we have previously used for the purification of n-bromoacetyl-3,3',5-triiodo-l-thyron ... | 1992 | 1443534 |
| [thyroid gland stimulation test in reinke's edema. a study of 28 patients]. | in order to clarify the correlation between reinke's oedema and thyreopathies, we performed a study on 28 patients affected by polypoid degeneration of the vocal cords. every patient had a complete check-up concerning the otorhino-laryngological either endocrinological aspects, in particular blood laboratory tests including the routine ones and ria for t3, t4, ft3, ft4, hgt, antibodies and tsh under stimulation of the trh. 22 patients showed to be affected by subclinical hypothyroidism; 5 patien ... | 1992 | 1443467 |
| everything you wanted to know about graves' disease. | graves' disease is an autoimmune thyroid disease characterized by a genetic predisposition, an increased incidence in young women, the presence of thyroid-stimulating immunoglobulins, and an uncertain etiology. the onset occasionally follows a frightening episode in the patient's life or separation from a loved one. on physical examination, the patient has a diffuse goiter with secondary hyperthyroidism, a noninfiltrative/infiltrative ophthalmopathy, and, on occasion, an infiltrative dermopathy. ... | 1992 | 1443362 |
| [thyroid gland disorders in elderly psychiatric patients]. | with 200 admissions of 192 patients to the psychogeriatric acute ward of the zwiefalten state mental hospital serial measurements of serum concentrations of basal tsh and, if necessary, of total t3 and total t4 were carried out with the trh tests performed. basal tsh was decreased in 16% of patients and in 2% it was increased. only three patients had manifest hyperthyroidism and one patient had marginal hypothyroidism. regardless of their psychiatric diagnosis, patients with manifest hyperthyroi ... | 1992 | 1441714 |
| repeat plateletpheresis: the effects on the donor and the yield. | requirements for hla ot otherwise matched single-donor platelets may sometimes require repeat plateletpheresis of an individual donor. aabb standards permit the repeat collection of platelets by plateletpheresis of a single donor at 48-hour intervals, whereas recent recommendations from england state that a donor should not donate platelets more often than 12 times a year. to assess the effects of repeat plateletpheresis on the donor, we have studied the hematological indices and the product yie ... | 1992 | 1441301 |
| systemic chemotherapy in regionally advanced bladder cancer. theoretical considerations and results. | integrating systemic chemotherapy into the treatment of patients with invasive bladder cancer, where the majority of deaths are from systemic relapse, is crucial if survival is to be improved. however, the global recommendation of a single treatment approach for all patients is becoming outdated as several groups have found that appropriately selected patients enjoy comparable survival whether treated by transurethral resection alone or by partial or radical cystectomy. thus, refining case selec ... | 1992 | 1441029 |
| [a case of myxedema heart with serial endomyocardial biopsy]. | we report a case of middle-aged woman with myxedema heart who presented congestive heart failure due to myocardial damage caused by myxedema, and showed reversible and irreversible myocardial change proven by serial endomyocardial biopsy. she was admitted to our hospital because of facial, peripheral edema and dyspnea. on admission, chest x-p revealed severe cardiomegaly (ctr 70%) and bilateral pleural effusion. electrocardiogram showed low voltage. echocardiogram and chest ct revealed a large a ... | 1992 | 1439254 |
| [evaluation of the thyroid function in healthy aged patients residing in 2 geriatric institutions]. | thyroid function assessment in elderly inpatients. it is not well known whether normal ageing might lead to an impairment of thyroid function. we evaluated 297 elderly inmates in two geriatric institutions. after having excluded from the study those who were affected by chronic hepatic or renal disease, were under pharmacologic treatment or had suffered from acute ailments during the previous two months, we assessed thyroid function by determining circulating t4, t3, rt3, ft4, ft3 and tsh in 130 ... | 1992 | 1439120 |
| [comparison of homologous and heterologous standard curves using tr-fia for the determination of total t4 in the rat]. | a homologous standard curve for rat t4 measurement has been compared to heterologous standard curves, by a time resolved fluoroimmunoassay (tr-fia). homologous standard curves have been carried out starting from rat serum in the absence of thyroid hormone by using samuel's technique. the security criteria (sensitivity, inter and intraassay precision, and accuracy), were measured and compared between the homologous and heterologous test. analysis of variance and slopes between homologous and hete ... | 1992 | 1439084 |
| [replication and gene expression of t4 phage dna]. | 1992 | 1438831 | |
| major depression and subclinical (grade 2) hypothyroidism. | subclinical hypothyroidism (sch) has been reported to occur in patients with a variety of affective syndromes. however, the clinical correlates of sch in patients with major depression have received limited attention. we therefore examined demographic, clinical and treatment response variables in a cohort of patients with unipolar, nonpsychotic major depression with and without sch. of 139 subjects, 19 had sch defined as an elevated basal tsh with normal circulating levels of t3 and t4. major de ... | 1992 | 1438646 |
| covalent modification of proteins by ligands of steroid hormone receptors. | retinoylation, acylation with retinoic acid (ra), is a covalent modification of proteins occurring in a variety of eukaryotic cell lines. in this study, we found that proteins in hl-60 cells were labeled by 17 beta-[3h]estradiol (e2), [3h]progesterone (pg), 1 alpha,25-dihydroxy[3h]vitamin d3 [1,25(oh)2d3], [125i]triiodothyronine (t3), [125i]thyroxine (t4), and [3h]prostaglandin e2 (pge2). all of these hormones, except pge2, are ligands of the steroid hormone receptor family. addition to the grow ... | 1992 | 1438281 |
| [serum triiodothyronine and thyroxine levels in children with celiac disease]. | the study was aimed at determining relationship between thyroid function and the type and degree of malabsorption. serum triiodothyronine (t3) and thyroxine (t4) levels were determined in children with celiac disease and the secondary malabsorption. hundred fifty five children aged between 6 months and 7 years were followed up 3 years. coeliac disease was diagnosed with classic interlaken criteria. all children were divided into three groups: group i--57 children aged between 6 months and 3 year ... | 1992 | 1437793 |
| molecular characterisation of a dna ligase gene of the extremely thermophilic archaeon desulfurolobus ambivalens shows close phylogenetic relationship to eukaryotic ligases. | a 3382 bp fragment containing a gene for a dna ligase from the extremely thermophilic, acidophilic, and facultatively anaerobic archaeon (archaebacterium) desulfurolobus ambivalens was cloned and sequenced. the deduced amino acid sequence (600 amino acids, 67619 molecular weight) showed 30-34% sequence identity with the atp-dependent eucaryal (eukaryotic) dna ligases of schizosaccharomyces pombe, saccharomyces cerevisiae, the human dna ligase i, and with the vaccinia dna ligase. distant similari ... | 1992 | 1437556 |
| effect of starvation and subsequent refeeding on thyroid function and release of hypothalamic thyrotropin-releasing hormone. | effects of starvation on thyroid function were studied in 5- to 6-week-old (r x u) f1 rats. starvation lowered plasma tsh in female, but not in male rats. plasma t4 and t3 levels decreased, whereas the dialysable t4 fraction increased during starvation. free t4 (ft4) levels decreased rapidly in females, but only after prolonged fasting in male rats. glucose decreased, and free fatty acid levels increased during starvation. peripheral trh levels did not change during food deprivation. since effec ... | 1992 | 1436373 |
| [clinical application of the biological variability data of the thyroid hormones]. | the selection of laboratory tests with greater effectiveness for identifying patients with thyroid disorders (sensitivity) from non affected subjects (specificity) is, at present, under discussion. the study of biological variation can contribute to point out the laboratory tests with greater effectiveness. | 1992 | 1434986 |
| [ventilation under high spinal anesthesia--the effect of hypotension]. | the ventilatory changes during the course of high spinal anesthesia and the effect of hypotension on ventilation during high spinal anesthesia were studied. spinal anesthesia with hyperbaric tetracaine was applied to 30 patients scheduled for elective surgery. patients breathed by mask for ten minutes at rest before and after receiving spinal anesthesia. respiratory parameters were measured in supine position during (1) pre-anesthetic period under resting condition, (2) anesthetic period when an ... | 1992 | 1433830 |
| plasmid models for bacteriophage t4 dna replication: requirements for fork proteins. | bacteriophage t4 dna replication initiates from origins at early times of infection and from recombinational intermediates as the infection progresses. plasmids containing cloned t4 origins replicate during t4 infection, providing a model system for studying origin-dependent replication. in addition, recombination-dependent replication can be analyzed by using cloned nonorigin fragments of t4 dna, which direct plasmid replication that requires phage-encoded recombination proteins. we have tested ... | 1992 | 1433501 |
| stimulatory effects of exogenous thyroid hormone and growth hormone on sn-glycerol-3-phosphate dehydrogenase activity in the genetic-hypothyroid mutant mouse cerebellum: restricted to the second 20 days of postnatal life. | we recently reported that by postnatal day 40 the activity of sn-glycerol-3-phosphate dehydrogenase (gpdh) was significantly depressed in the cerebellum of genetic-hypothyroid mutant mice. this mutant mouse-gpdh combination was used in the present study to define the critical time period during which thyroid hormone (t4) and growth hormone (gh) are essential for maturation of bergmann glial cells. our findings are that (a) induction of gpdh activity in the bergmann glial cell is dependent on t4, ... | 1992 | 1431890 |
| the role of chorionic gonadotropin in transient hyperthyroidism of hyperemesis gravidarum. | biochemical evidence of hyperthyroidism is frequently encountered in hyperemesis gravidarum, but its relationship to the cause of hyperemesis is unknown. we studied the relationship of serum hcg, thyroid function, and severity of vomiting among 57 hyperemesis patients and 57 controls matched for gestational age. tsh was suppressed in 60% of hyperemesis patients and 9% of controls. hcg correlated directly with free t4(r = 0.45, p < 0.001) and inversely with tsh (r = -0.48, p < 0.001). hyperemesis ... | 1992 | 1430095 |
| adjuvant radiotherapy in high stage transitional cell carcinoma of the renal pelvis and ureter. | this review was undertaken to assess the influence of adjuvant radiation therapy on failure patterns and survival in high stage transitional cell carcinoma of the renal pelvis or ureter. ninety-four patients with transitional cell carcinoma of the renal pelvis or ureter were retrospectively reviewed. twenty-six had american joint commission stage t3 or t4 n0/+, m0 disease and underwent curative resections (median follow-up 13.5 months, range 3-311). local failure was defined as recurrence in the ... | 1992 | 1429099 |
| a role for thyroid hormone in the induction of ovulation and corpus luteum function. | we have previously demonstrated that thyroid hormone synergizes with follicle-stimulating hormone (fsh) to exert stimulatory effects on granulosa cell differentiation and function, suggesting that it plays a physiological role in amplifying fsh-mediated differentiation of granulosa cells. the adequate differentiation of these cells, followed by normal follicle development, is indispensable for ovulation and subsequent corpus luteum formation. thus, in the present studies, the clinical implicatio ... | 1992 | 1427622 |
| cloning, sequencing, overproduction, and purification of m. cvibi (gantc) methyltransferase from chlorella virus nc-1a [corrected]. | we have cloned and sequenced the cvibim gene from chlorella virus nc-1a by selecting for the modification phenotype. the modification gene was cloned on a 7-kb bamhi fragment inserted into the bamhi site of the puc13 plasmid. the cvibim gene was localized at the 3' end of this fragment. sequencing of this region revealed a large open reading frame that codes for methyltransferase (mtase; symbol m.) (predicting 260 amino acids). m.cvibi (gantc) aa sequence is homologous to m.dam(gatc), m.dpnii(ga ... | 1992 | 1427082 |
| chronic fasting reduces the response of the thyroid to growth hormone and tsh, and alters the growth hormone-related changes in hepatic 5'-monodeiodinase activity in rainbow trout, oncorhynchus mykiss. | chronically fasted rainbow trout (oncorhynchus mykiss) had significantly lower plasma l-thyroxine (t4) and triiodo-l-thyronine (t3), and higher plasma growth hormone (gh) concentrations than fed animals. fasted and fed trout were administered bovine thyrotropic hormone (btsh), native ovine gh (ogh), or recombinant human gh (rhgh) alone, or gh in combination with btsh to further study the effects of food deprivation on the activity of the pituitary-thyroid axis and on the control of hepatic t3 pr ... | 1992 | 1426938 |
| unexpected intron location in non-vertebrate globin genes. | the caenorhabditis elegans and artemia t4 globin sequences are highly homologous with other invertebrate globins. the intron/exon patterns of their genes display a single intron in the e and g helices respectively. precoding introns in multirepeat globins are inserted in homologous positions. comparison of the intron/exon patterns in the known globin gene sequences demonstrates that they are more diverse than first expected but nevertheless can be derived from an ancestral pattern having 3 intro ... | 1992 | 1426238 |
| risks of alternative nutrition in infancy: a case report of severe iodine and carnitine deficiency. | a 7.5-month-old infant with failure to thrive, developmental delay, muscular hypotonia, a visible goitre and severe osteopenia is described. laboratory examination revealed a markedly increased serum tsh with low free t4, severe iodine and carnitine deficiency. the infant was breastfed until the age of 2.5 months and was then given a mixture of almond extract in water. the mother is a strict vegan and the father a lactovegetarian. the nutritional intake of the child was severely depleted in calo ... | 1992 | 1425805 |
| a case of hypothyroidism with simultaneous presence of stimulating type anti-thyrotropin (tsh) receptor antibodies and anti-thyroxine (t4) autoantibodies. | we have examined a hypothyroid patient with stimulating type anti-thyrotropin (tsh) receptor antibodies and without blocking type anti-tsh receptor antibodies. although she had high serum tsh (240 microu/ml) and low free triiodothyronine (ft3, 0.49 pg/ml) concentrations, which agree with physical findings of hypothyroidism, she had an unusually high free thyroxine (ft4) concentration (3.56 ng/dl). incubation of her serum with 125i-t4, followed by precipitation with 12.5% polyethylene glycol (peg ... | 1992 | 1425450 |
| reversion of thyrotoxic atrial fibrillation in hypothyroid state after radioiodine treatment. | twenty patients with thyrotoxic basedow's disease complicated by atrial fibrillation lasting more than one month despite treatment with antithyroidal drugs were treated with radioiodine supplemented with an antithyroidal drug or inorganic iodine. we classified the 20 patients on the basis of atrial fibrillation reversion into two groups, one with reversion (group i) and the other without reversion (group ii). in all 12 patients in group i, t4 and t3 decreased to hypothyroid levels in 3.2 +/- 1.3 ... | 1992 | 1425447 |
| thyroid hormone-dependent redistribution of the 55-kilodalton monomer of protein disulfide isomerase in cultured glial cells. | in addition to the effects of thyroid hormone that are mediated through interaction with chromatin-associated receptors, t4 modulates the activity of the cellular content of the membrane-associated protein type ii iodothyronine 5'-deiodinase (5'd-ii) by regulating its degradation through an actin-dependent extranuclear mechanism. under the influence of thyroid hormone, the substrate-binding subunit of 5'd-ii is translocated from the plasma membrane to an intracellular microfilament-associated po ... | 1992 | 1425439 |
| continuous infusion of interleukin-1 beta induces a nonthyroidal illness syndrome in the rat. | we studied the effects of continuous administration of recombinant human interleukin-1 beta (il-1) on pituitary-thyroid function. rats were equipped with minipumps loaded with either il-1 (delivery rate, 0.5, 2.0, or 4.0 micrograms/day, ip, for 1 week) or saline. infusion of 2.0 and 4.0 micrograms il-1/day caused a significant decrease in plasma free t4 levels during the first 2-4 days, whereas plasma total t4 levels and t4 binding were significantly lowered throughout the week of the study. the ... | 1992 | 1425414 |
| [organ-preserving therapy in invasive bladder carcinoma]. | with the aim of organ preservation, transurethral resection with subsequent radiotherapy (until 1985) or combined radio- and chemotherapy (since 1986) was undertaken as part of a prospective trial in 175 consecutive patients (137 men, 38 women; mean age 65 [31-90] years) with invasive bladder carcinoma, tumour stage t1-4 n0-3 m0. all patients had a transurethral resection, followed 2-6 weeks later by definitive radiotherapy at a dose of 50.4 gy to the bladder in 28 fractions. 85 patients simulta ... | 1992 | 1425303 |
| feedback regulation of thyrotropin-releasing hormone gene expression by thyroid hormone in the hypothalamic paraventricular nucleus. | hypothyroidism caused by chemical or surgical thyroidectomy or hypophysectomy causes a substantial increase in the content of thyrotropin-releasing hormone (trh) mrna and protrh exclusively in cells of the medial and periventricular paravocellular subdivisions of the hypothalamic paraventricular nucleus (pvn). this response may be important to raise the anterior pituitary thyrostat to promote increased secretion of thyroid-stimulating hormone (tsh) and to induce the secretion of a more biologica ... | 1992 | 1425022 |
| thyrotropin-releasing hormone: pharmacokinetic and pharmacodynamic properties in chronic renal failure. | the pharmacokinetics of thyrotropin-releasing hormone (trh) were determined following a single i.v. administration in ten patients with chronic renal failure (crf) maintained on chronic hemodialysis and in six normal subjects. a trh-test (200 micrograms) was performed in all subjects on nondialysis days and was followed by sequential venous blood sampling at 0, 2, 5, 10, 20, 30 and 60 min. plasma trh and serum concentrations of tsh, t4, ft4 and t3 were measured by specific and sensitive ria's. s ... | 1992 | 1424308 |
| the effect of growth hormone administration in growth hormone deficient adults on bone, protein, carbohydrate and lipid homeostasis, as well as on body composition. | the effect on bone, protein, carbohydrate and lipid homeostasis as well as body composition of the administration of growth hormone to adult patients with growth hormone deficiency was studied. | 1992 | 1424196 |
| effect of selenium supplementation on thyroid hormone metabolism in an iodine and selenium deficient population. | severe selenium deficiency has been documented in northern zaïre, already known as one of the most iodine deficient regions in the world and characterized by a predominance of the myxoedematous form of cretinism. this has been attributed to the double deficiency of essential trace elements. a short selenium supplementation programme was conducted in this area to evaluate the effects of a selenium supplementation on thyroid diseases. | 1992 | 1424183 |
| the effect of iodide on serum thyroid hormone levels in normal persons, in hyperthyroid patients, and in hypothyroid patients on thyroxine replacement. | to clarify the duration and the extent of the antithyroid effect of iodides in hyperthyroidism, and to investigate whether iodides have an additional peripheral effect on the metabolism of thyroid hormones, as has been reported for some organic iodine compounds. | 1992 | 1424182 |
| preserved thyroidal secretion of thyroxine in acromegalic patients with suppressed hypophyseal secretion of thyrotrophin. | we have assessed the mechanisms which maintain euthyroidism in acromegalic patients despite the suppression of thyrotrophin (tsh) secretion. | 1992 | 1424168 |
| lipid-associated sialic acid, squamous cell carcinoma antigen, carcinoembryonic antigen, and lactic dehydrogenase levels as tumor markers in squamous cell carcinoma of the head and neck. | circulating tumor markers are valuable adjuncts in the management of several malignant lesions, including germ cell tumors and adenocarcinomas of the breast, colon, prostate, and ovary. however (to the authors' knowledge), currently, no serologic markers have been shown to have prognostic value for patients with squamous cell carcinomas of the head and neck (scchn). | 1992 | 1423179 |
| long-term treatment results of postoperative radiation therapy for advanced stage oropharyngeal carcinoma. | the authors report the long-term treatment results for advanced stage base of tongue (bot) and tonsillar fossa (tf) carcinomas treated with surgery and postoperative radiation therapy (rt) at memorial sloan-kettering cancer center. | 1992 | 1423170 |
| serial thyroid hormone concentrations in healthy euthyroid dogs, dogs with hypothyroidism, and euthyroid dogs with atopic dermatitis. | serum thyroxine (t4) and 3,5,3'-triiodothyronine (t3) concentrations were determined every 3 h for 12 h beginning at 8 a.m. in 20 healthy euthyroid dogs, 19 dogs with hypothyroidism, and 18 euthyroid dogs with atopic dermatitis. status of thyroid function was based on history, physical findings, results of thyrotropin response testing, and requirement for thyroid hormone replacement therapy. mean serum t4 and t3 concentrations did not vary significantly between blood samplings within each of the ... | 1992 | 1422786 |
| treatment of advanced localised prostatic cancer by orchiectomy, radiotherapy, or combined treatment. a medical research council study. urological cancer working party--subgroup on prostatic cancer. | a total of 277 patients with apparently localised prostatic cancer (t2-t4 nxmo) were allocated at random to receive radiotherapy alone (88), orchiectomy alone (90) and combined therapy (99) between 1980 and 1985. the main outcome measures were survival, time to appearance of metastases and treatment of local disease progression by further transurethral resection. orchiectomy, whether alone or with radiotherapy, produced a significant delay in detection of metastases when compared with radiothera ... | 1992 | 1422689 |
| referral patterns of patients with oral squamous cell carcinoma, australia. | early diagnosis is an important factor affecting the prognosis of patients with oral cancer. delays in diagnosis have been variously reported as being linked to the patient, the clinician or both. the purpose of this study was to investigate the referral patterns of patients with oral cancer by medical and dental practitioners, in order to assess the delays in diagnosis and to establish the reasons for these delays. 51 consecutive patients diagnosed with oral mucous membrane squamous cell carcin ... | 1992 | 1422466 |
| characteristics of long-term human thyroid peroxidase autoantibody secretion in scid mice transplanted with lymphocytes from patients with autoimmune thyroiditis. | we have explored scid mice as an in vivo model to study lymphocyte function and autoantibody production in patients with autoimmune thyroiditis and thyroid peroxidase (htpo) autoantibodies. patient's peripheral blood mononuclear cells (pbmc) were transplanted into scid mice via intraperitoneal injections and human immunoglobulin g (higg) and thyroid autoantibody levels in the murine sera were monitored for a minimum of 3 months after transplantation. human igg reached maximum serum levels of > 3 ... | 1992 | 1422261 |
| the fetus in maternal hyperthyroidism. | a recent article in the new england journal of medicine reported the successful diagnosis and treatment of fetal goitrous hypothyroidism in a mother with graves' disease. the fetus is being recognized as an important patient in its own right in terms of thyroid disease. the fetal thyroid system develops independently of the normal maternal thyroid axis. presence of feedback suppression of tsh by t4 has been demonstrated in a 35-week fetus. information learned from congenital hypothyroidism sugge ... | 1992 | 1422235 |
| diagnosis and management of graves' disease in pregnancy. | most of the hyperthyroidism seen in association with pregnancy is graves' disease. the best treatment is prevention. for most patients there is an opportunity to treat the hyperthyroidism decisively with radioiodine or surgery before the patient becomes pregnant. pregnancy complicated by hyperthyroidism is often a consequence of the conscious decision to treat hyperthyroidism in women in the childbearing years with antithyroid drugs. propylthiouracil (ptu) is the preferred treatment for hyperthy ... | 1992 | 1422234 |
| peroxidase and coupling activities of thyroid peroxidase in benign and malignant thyroid tumor tissues. | the coupling activity of thyroid peroxidase (tpo) in thyroid glands from patients with benign adenoma, papillary carcinoma, and diffuse goiter (graves' disease) was measured for the first time, in addition to the peroxidase activity of these tissues. the peroxidase activity of tpo in the mitochondria-microsomes fraction was measured with guaiacol or iodide as the second substrate. in the case of papillary carcinoma, the mean protein-based specific activity obtained by the guaiacol assay was abou ... | 1992 | 1422230 |
| a new 3d hn(ca)ha experiment for obtaining fingerprint hn-halpha peaks in 15n- and 13c-labeled proteins. | a new 3d 1h-15n-13c triple resonance experiment is presented that provides in-phase absorptive cross peaks between amide protons and alpha-protons of the same and the sequentially preceding residue. the experiment yields similar connectivities as those described previously by montelione and wagner (1990a) (j. magn. reson., 87, 183-188) and kay et al. (1991) (j. magn. reson., 91, 84-92). however, the pulse sequence was designed to minimize the time that transverse coherence of the 13calpha nucleu ... | 1992 | 1422153 |
| a further study of the t4 ligase-catalyzed joining of dna at base-paired ends. 1972. | 1992 | 1422025 | |
| intrathyroidal activated (ia+) t-lymphocyte cd+ subsets and b cells in graves' hyperthyroidism respond rapidly to propylthiouracil therapy: demonstration using fine needle aspirates and two-colour laser flow cytometry. | using a rapid (whole blood lysis) single laser microfluorocytometric technique that permitted the simultaneous analysis of two monoclonal antibody surface markers tagged with different fluorescent dyes, the intrathyroidal (it) and peripheral blood (pb) activated [ia+ = dr+] t-lymphocyte cd3+ subsets and [f(ab')2+] b cells were studied in hyperthyroid patients with graves' disease (gd) before and after 1-4 months of propylthiouracil (ptu) therapy. it lymphocytes were obtained by serial fine needl ... | 1992 | 1420805 |
| the structural and functional organization of the baseplate distal part of t4 bacteriophage. | the structural organization of the baseplate distal part of bacteriophage t4 has been studied. the investigations resulted in revealing the functional role of gene products '11' and '12' in the course of baseplate reorganization and dna injection. a new model of 'arrangement' of gp12 in the intact particle is suggested. a functional interrelationship between long fibers of the phage and the protein complex of baseplate distal part has been established. | 1992 | 1420274 |
| a differential scanning calorimetric study of the thermal unfolding of mutant forms of phage t4 lysozyme. | in continuation of our earlier work on the effects of amino acid replacements on the thermodynamics of the thermal unfolding of t4 lysozyme [kitamura, s., & sturtevant, j. m. (1989) biochemistry 28, 3788-3792; connelly, p., ghosaini, l., hu, c.-q., kitamura, s., tanaka, a., & sturtevant, j. m. (1991) biochemistry 30, 1887-1891; hu, c.-q., kitamura, s., tanaka, a., & sturtevant, j. m. (1992) biochemistry 31, 1643-1647], we report here a study by differential scanning calorimetry of the effects of ... | 1992 | 1420185 |
| roles of cys148 and asp179 in catalysis by deoxycytidylate hydroxymethylase from bacteriophage t4 examined by site-directed mutagenesis. | the proposed roles of cys148 and asp179 in deoxycytidylate (dcmp) hydroxymethylase (ch) have been tested using site-directed mutagenesis. ch catalyzes the formation of 5-(hydroxymethyl)-dcmp, essential for dna synthesis in phage t4, from dcmp and methylenetetrahydrofolate. ch resembles thymidylate synthase (ts), an enzyme of known three-dimensional structure, in both amino acid sequence and the reaction catalyzed. conversion of cys148 to asp, gly, or ser decreases ch activity at least 10(5)-fold ... | 1992 | 1420151 |
| technical and light microscopic comparison of four different small-diameter catheters used for continuous spinal anesthesia. | recently several manufacturers developed ultra-thin catheters for continuous spinal anesthesia. their use may be associated with technical problems. | 1992 | 1419943 |
| primary gastric non-hodgkin's lymphoma. a retrospective clinico-pathological study. | prognostic factors and treatment results were analysed in 72 consecutive patients with primary gastric lymphoma treated between 1970 and 1985. there were 37 patients in stage ie, 17 in iie, 3 in iies and 15 in stage iv. histopathological re-evaluation and classification according to the tnm system were performed. we found that disseminated disease (stage iv), serosal penetration (t3), involvement of adjacent organs (t4) and extensive abdominal lymph node involvement (n3) were poor prognostic fac ... | 1992 | 1419098 |
| [nourishing yin action of the prepared rhizoma rehmanniae]. | the results of the experiment show that the prepared rhizomae rehmanniae can obviously improve the change of weight, quality of drinking water, urine and serum level of t3, t4 and ad of the hyperthyroid yin deficient rats, and thus administers to the nourishing of yin. the action of nourishing the kidney yin is related with improving the serum level of ad in the body. | 1992 | 1418567 |
| effect of insulin and thyroxine on catalase, glutathione-s-transferase, gsh and gssg in alloxan diabetic rat red cells. | the levels of catalase (cat), glutathione-s-transferase (gst), reduced glutathione (gsh) and oxidised glutathione (gssg) were measured in red blood cells from control (c) and diabetic rats (d). diabetes was induced by alloxan administration and diabetic rats were treated with insulin (d+i) and thyroxine (d+t4). on the third day of insulin withdrawal the cat activity increased significantly. the gst activity showed an increase in d and d+i for one week, thyroxine treatment to d rats resulted in m ... | 1992 | 1417913 |
| [hiv infection in infants under 15 months of age. value of the p-24 antigen as a prognostic marker]. | we have studied 65 children, born to hiv seropositive mothers, during the first quarter of life and afterwards every three-months. during this time, ten of the children (13%) became infected with the virus. the study of p24 antigen during the first 15 months of life showed an inverse relationship between the permanent presence of p24 antigen in the serum and the absence of anti-p24 antibodies. since both markers were related to a bad prognosis, it is useful to carry-out the routine study of p24 ... | 1992 | 1416532 |
| respiratory muscle strength in hyperthyroidism before and after treatment. | we undertook this study to investigate respiratory muscle strength in relation to thyroid function in 20 thyrotoxic patients and in a group of 20 normal subjects matched for age and sex. global respiratory muscle strength was assessed by measuring mouth pressure during maximal static inspiratory (pimax) and expiratory (pemax) efforts. we also measured vc, fvc, and fev1 as well as thyroid-related hormones (t3, t4, tsh). measurements were made once in normal subjects and twice in thyrotoxic patien ... | 1992 | 1416391 |
| serum free and total iodothyronine concentrations in dogs with hyperadrenocorticism. | serum concentrations of total and free thyroxine (t4 and ft4, respectively), 3,5,3'-triiodothyronine (t3), 3,3',5'-triiodothyronine (reverse t3) were measured in 42 dogs with hyperadrenocorticism, and were compared with values determined in clinically normal dogs. mean total t4 concentration in dogs with hyperadrenocorticism (14.3 nmol/l) was significantly (p less than 0.001) lower than the normal value (25.7 nmol/l), with 38% of the dogs having low serum t4 concentration. although 16 (38%) of t ... | 1992 | 1416369 |
| spinal stimulation to locate preganglionic neurons controlling the kidney, spleen, or intestine. | the organization of sympathetic preganglionic neurons may be a substrate for selective control of sympathetic outflow to different vascular beds. this study was done to determine the spinal segments containing preganglionic neurons controlling discharge of renal, splenic, and mesenteric postganglionic nerves. in urethan-anesthetized rats, preganglionic neurons were stimulated by microinjecting d,l-homocysteic acid (3 nl, 0.17 m) into the lateral gray matter of the third thoracic (t3) to the four ... | 1992 | 1415750 |
| ontogeny of intestinal lactase: posttranslational regulation by thyroxine. | to assess the molecular mechanisms underlying the regulation of lactase ontogeny by thyroxine (t4), we performed an in vivo study of lactase catalytic activity, synthesis, subunit structure, degradation, and enterocyte migration rates in propylthiouracil-induced hypothyroid rat pups, hypothyroid pups injected with t4, and normally weaned rats. although lactase catalytic activity remained elevated in the hypothyroid rats and declined normally in the other two groups, lactase synthesis was constan ... | 1992 | 1415712 |
| thyroid hormone effects on cardiac gene expression independent of cardiac growth and protein synthesis. | prior studies have demonstrated the importance of hemodynamic loading in mediating thyroxine (t4)-induced cardiac hypertrophy. direct cellular effects of thyroid hormone have been implicated in modulating the expression of the myosin heavy chain (mhc) genes and the slow sarcoplasmic reticulum calcium adenosine triphosphatase (sr ca(2+)-atpase) gene. in the present report, administration of t4 for 72 h did not stimulate growth of the hemodynamically unloaded heterotopic isograft. the synthetic ra ... | 1992 | 1415533 |
| thermogenesis after surgery: effect of perioperative heat conservation and epidural anesthesia. | body temperature, respiratory gas exchange, and plasma catecholamines were determined before and after surgery in three groups [control (c), warmed (w), and epidural (e) who received local anesthetic at t4-s5 dermatomes during and for 24 h after surgery] of patients undergoing colonic surgery under general anesthesia. at the end of surgery, group w were nursed in an ambient temperature of 28-30 degrees c, whereas the others were at 20-23 degrees c for a period of 24 h. core (tc) and dorsal hand ... | 1992 | 1415523 |
| [the effect of repeated administration of carbetocin (depotocin, spofa) in the first days postpartum on levels of thyroxine, triiodothyronine, 17 beta-estradiol, progesterone and on fertilization]. | application of new procedures in the sphere of the control of sexual functions requires an extension of present knowledge of postparturient endocrinium or endogenic factors comprised in postparturient physiology of sexual activity. according to recent data, oxytocin, besides its uterotonic and luteolytic activity, acts as an ovarian factor in the local intrafollicular regulation of stereidogenesis and as a modulator of uterine secretion of prostaglandines. based on present knowledge of oxytocin ... | 1992 | 1413401 |
| atomic force microscopy imaging of t4 bacteriophages on silicon substrates. | a new atomic force microscope incorporating microfabricated cantilevers and employing laser beam deflection for force detection has been constructed and is being applied to studies of biological material. in this study, t4 bacteriophage virus particles were deposited from solution onto electronic-grade flat silicon wafers and imaged in air with the microscope. microliter droplets of the solution were deposited and either allowed to dry or removed with blotting paper. the images show both isolate ... | 1992 | 1413249 |
| cardiac systolic time intervals and thyroid hormone levels during treatment of hypothyroidism. | this study was undertaken to compare results of modern serum thyroid hormone assays with cardiac systolic time intervals (sti) during thyroxine treatment in hypothyroid patients. the patients were assessed clinically (billewicz index) and the sti and serum thyrotropin (tsh), total and free thyroxine (t4) and total and free triiodothyronine (t3) were determined in 16 hypothyroid women (group i) treated with 50 micrograms increments of thyroxine, and in 13 women who had a history of thyroid carcin ... | 1992 | 1411259 |
| [study of thyroid function in the organ donor during brain death]. | to determine the thyroid function in organ donors undergoing cerebral death and evaluate the effects that these possible alterations may exert on the early function of renal graft. | 1992 | 1410752 |
| hypothyroidism following radiotherapy for head and neck cancer: multivariate analysis of risk factors. | the incidence of hypothyroidism following radiotherapy for head and neck cancer is 10-45% in several series. in order to identify possible risk factors a multivariate analysis was performed in 221 patients previously treated with either radiotherapy alone or combined with surgery or chemotherapy. diagnostic procedures included: total t3 and t4, free t4 and tsh measured by radioimmunoassay, and thyroid scintigraphy. the absolute rate of hypothyroidism was 41.17% (subclinical 26.24%, clinical 14.9 ... | 1992 | 1410587 |
| clinical experience with a single field rotational total skin electron irradiation technique for cutaneous t-cell lymphoma. | between october 1981 and december 1989, 44 patients with cutaneous t-cell lymphoma (ctcl) were treated with a single field rotational total skin electron irradiation (rtsei) technique developed in the mcgill university, department of radiation oncology. only 11 (25%) of the 44 patients had received no prior treatment. three-quarters (33/44) had advanced (t3 or t4) disease. complete responses were seen in 32/44 (73%) of patients (91% t2, 71% t3 and 58% t4), but only 3/11 (27%) of patients with t2 ... | 1992 | 1410569 |
| ligation-anchored pcr: a simple amplification technique with single-sided specificity. | a simple, efficient, and sensitive technique has been developed for amplification of cdnas encoding molecules with 5' regions of unknown sequence. in this ligation-anchored pcr, t4 rna ligase is used to covalently link an "anchor" oligonucleotide to first-strand cdnas. these anchored cdnas are then amplified by using one pcr primer specific for the anchor and another specific for a sequence within the molecule of interest. the anchor oligonucleotide has been especially designed to facilitate sub ... | 1992 | 1409706 |
| role of the basic amino acid cluster and glu-23 in pyrimidine dimer glycosylase activity of t4 endonuclease v. | t4 endonuclease v [endodeoxyribonuclease (pyrimidine dimer); deoxyribonuclease (pyrimidine dimer), ec 3.1.25.1] initiates repair of damaged dna by hydrolysis of the n-glycosyl bond at the 5' side of a pyrimidine photodimer in double-stranded dna. to study one of the active sites of t4 endonuclease v, systematic site-directed mutagenesis was performed on the synthetic t4 endonuclease v gene, in parallel with three-dimensional structure analysis by x-ray crystallography. the mutant proteins were e ... | 1992 | 1409651 |
| multitarget-ribozyme directed to cleave at up to nine highly conserved hiv-1 env rna regions inhibits hiv-1 replication--potential effectiveness against most presently sequenced hiv-1 isolates. | several mono-, di-, tetra-, penta- and nonaribozymes were developed. these multitarget-ribozymes were targeted to cleave hiv-1 env rna at up to nine different conserved sites. each multitarget-ribozyme consisted of a chain of up to nine hammerhead motifs, each flanked by a different targeting sequence. the multitarget-ribozymes were functional in vitro and gave rise to multiple, specific partial and/or complete rna digestion products. per rna copy, multitarget-ribozymes were more efficient than ... | 1992 | 1408760 |
| synergistic effects of thyroxine and dexamethasone on enzyme ontogeny in rat small intestine. | the synergistic effects of dexamethasone (dex) and thyroxine (t4) on the postnatal maturation of the 13-d-old rodent small intestine has been studied. previous studies have shown that hydrocortisone and t4 produced a synergistic response in enzyme maturation. however, t4 elevates corticosteroid-binding globulin, which reduces the clearance of hydrocortisone. thus, the apparent synergy between t4 and hydrocortisone may have been due to increased glucocorticoid availability. dex, which does not bi ... | 1992 | 1408467 |
| [results of curative or non-curative nephrectomy for renal cell carcinoma invading adjacent organs]. | we reviewed 12 patients who had undergone curative or non-curative nephrectomy for renal cell carcinoma invading adjacent organs (stage t4). 83 patients with renal cell carcinoma confined within the perirenal fascia (t1-t3) who had undergone nephrectomy served as controls. of the 12 patients with t4 tumor 6 had undergone simultaneous excision of involved adjacent organs (hemicolectomy in 4, resection of the tail of pancreas in 5, splenectomy in 2). at operation 6 patients with t4 tumor had dista ... | 1992 | 1405162 |
| [hypergastrinemia and type a gastritis in basedow's disease]. | hypergastrinemia is a very important clinical condition for the reason that a growing body of evidence obtained from animal and human experiments has revealed gastric carcinoids induced by hypergastrinemia. we investigated 35 patients with basedow's disease (bd) to elucidate the mechanism of hypergastrinemia associated with bd as well as the relationship between type a gastritis and bd. fasting serum gastrin levels in bd (296.1 +/- 251.4 pg/ml; mean +/- s.d.) were significantly (p less than 0.00 ... | 1992 | 1404986 |
| monoclonal antibody ki-67 in the study of the proliferative activity of bladder carcinoma. | we studied the proliferative activity of bladder carcinoma using monoclonal antibody ki-67, which is able to stain a nuclear antigen exclusively present in cells in the cell cycle, that is with activated deoxyribonucleic acid (dna). we used this immunohistochemical technique on neoplastic tissue removed by transurethral resection from 101 patients. a significant correlation was observed (p less than 0.003) between cells with activated dna and histological grading, even though within the context ... | 1992 | 1404626 |