Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| antimicrobial activity and biodiversity of endophytic fungi in dendrobium devonianum and dendrobium thyrsiflorum from vietman. | endophytic fungi are rich in orchids and have great impacts on their host plants. 53 endophytes (30 isolates from dendrobium devonianum and 23 endophytic fungi from d. thyrsiflorum) were isolated, respectively, from roots and stems of dendrobium species. all the fungi were identified by way of morphological and/or molecular biological methods. 30 endophytic fungi in d. devonianum were categorized into 11 taxa and 23 fungal endophytes in d. thyrsiflorum were grouped into 11 genera, respectively. ... | 2010 | 21181405 |
| an alternate method of classifying allergic bronchopulmonary aspergillosis based on high-attenuation mucus. | allergic bronchopulmonary aspergillosis (abpa) is classified radiologically based on the findings of central bronchiectasis (cb) and other radiologic features (orf). however, the long-term clinical significance of these classifications remains unknown. we hypothesized that the immunological activity and outcomes of abpa could be predicted on hrct chest finding of high-attenuation mucus (ham), a marker of inflammatory activity. in this study, we evaluate the severity and clinical outcomes of abpa ... | 2010 | 21179536 |
| secretion stress and fungal pathogenesis: a new, exploitable chink in fungal armor? | 2011 | 21178506 | |
| successful, combined long-term treatment of cerebral aspergillosis in a liver transplant patient. | invasive aspergillosis has long been recognized as one of the most significant and often fatal opportunistic fungal infections in liver transplant recipients. we report a case of a liver transplant recipient who developed an aspergillus fumigatus brain abscess that produced significant neurologic symptoms. the patient was managed successfully with a combination of surgery and medical treatment with voriconazole. to our knowledge, this is the second such case reported in the literature. | 2010 | 21178487 |
| manipulation of host angioneogenesis: a critical link for understanding the pathogenesis of invasive mold infections? | despite progress over the last decade, opportunistic mold infections continue to be associated with high rates of morbidity and mortality in immunocompromised patients. given the propensity of molds to invade blood vessels, vasculopathy may be a barrier to effective delivery of antifungal drugs to infected tissue. in a recent study (ben-ami r et al. blood, 2009), we found that a. fumigatus suppresses endothelial cell migration, differentiation and capillary tube formation both in vitro and in an ... | 2010 | 21178441 |
| fungal hydrophobins form a sheath preventing immune recognition of airborne conidia. | aspergillus fumigatus is the most ubiquitous and the most threatful airborne fungal pathogen. in the atmosphere, there is thousands of conidia/m (3) originating from more than hundred fungal genera, which enter the host through the respiratory system and are eliminated by the innate immune defences. but how do a. fumigatus conidia survive long enough in the lung withstanding the host killing reactions? we demonstrated recently the role of the spore-surface rodlet layer made up of hydrophobic pro ... | 2010 | 21178439 |
| bioluminescent fungi for real-time monitoring of fungal infections. | novel luciferase reporters have been developed that allow real-time monitoring of infections by the fungal pathogens candida albicans and aspergillus fumigatus. although these reporters still suffer limitations in the context of invasive infections, they provide unprecedented tools to monitor superficial infections and the efficacy of antifungal drugs or vaccines. in particular, the sensitivity and ease of detection of the cell-surface gaussia princeps luciferase developed for c. albicans should ... | 2010 | 21178436 |
| characterization of a 5-azacytidine-induced developmental aspergillus fumigatus variant. | the hypomethylating agent 5-azacytidine (5ac) is widely used in patients at risk of invasive mycoses. we sought to determine whether 5ac affects the developmental competence and virulence of aspergillus fumigatus. incubation of a. fumigatus strain 293 with 5ac induced high-frequency conversion to a fluffy-variant (af293 (fl) ). the conidiation defect was bypassed by exposing af293 (fl) to light during the initial 18 hours of growth on solid media. transcriptional profiling revealed differential ... | 2010 | 21178435 |
| characterization of a polyketide synthase in aspergillus niger whose product is a precursor for both dihydroxynaphthalene (dhn) melanin and naphtho-γ-pyrone. | the genome sequencing of the fungus aspergillus niger uncovered a large cache of genes encoding enzymes thought to be involved in the production of secondary metabolites yet to be identified. identification and structural characterization of many of these predicted secondary metabolites are hampered by their low concentration relative to the known a. niger metabolites such as the naphtho-γ-pyrone family of polyketides. we deleted a non-reducing pks gene in a. niger strain atcc 11414, a daughter ... | 2010 | 21176790 |
| [n-cadherin as a receptor for adhesion and endocytosis of aspergillus fumigatus by human umbilical vein endothelial cells]. | to study the receptor for adhesion and endocytosis of aspergillus fumigatus hyphae by human umbilical vein endothelial cells (huvec). | 2010 | 21176502 |
| factors influencing the performance level of candida mannan antigen testing in allogeneic stem cell transplant recipients not receiving fluconazole prophylaxis. | a. nihtinen, v.-j. anttila, m. richardson, t. ruutu, e. juvonen, t. meri, l. volin. factors influencing the performance level of candida mannan antigen testing in allogeneic stem cell transplant recipients not receiving fluconazole prophylaxis. transpl infect dis 2011: 13: 266-272. all rights reserved abstract: in this study, we evaluated the value of the platelia(®) candida mannan antigen (ag) sandwich enzyme-linked immunosorbent assay test in the diagnosis of invasive candidiasis (ic) and the ... | 2010 | 21176021 |
| [radiologic picture of maxillary sinus aspergilloma]. | mycotic infection of paranasal sinus could be the etiological factor of chronic sinusitis. the increase in number of fungal sinusitis cases have been reported recently among nonimmunocompromised patient after endodontic treatment of maxillary teeth. nonspecific clinical signs and incorrect radiologic pictures interpretation as well as loss of therapeutic standards seems to be the cause of false negative diagnosis and difficulties in treatment of fungal sinusitis. | 2010 | 21171309 |
| multifocal nodular periostitis associated with prolonged voriconazole therapy in a lung transplant recipient. | we report a case of painful, nodular periostitis in a lung transplant recipient on long-term voriconazole therapy. symptoms, signs, and laboratory abnormalities resolved quickly after drug withdrawal. the presentation more closely resembles periostitis deformans than hypertrophic osteoarthropathy, suggesting that the fluoride moiety of voriconazole may be pathogenic for this condition. clinicians should be aware of this association. | 2011 | 21169844 |
| natural product-guided discovery of a fungal chitinase inhibitor. | natural products are often large, synthetically intractable molecules, yet frequently offer surprising inroads into previously unexplored chemical space for enzyme inhibitors. argifin is a cyclic pentapeptide that was originally isolated as a fungal natural product. it competitively inhibits family 18 chitinases by mimicking the chitooligosaccharide substrate of these enzymes. interestingly, argifin is a nanomolar inhibitor of the bacterial-type subfamily of fungal chitinases that possess an ext ... | 2010 | 21168763 |
| aspergillus spondylodiscitis in solid organ transplant recipients. | transplantation practices have had a significant effect on the epidemiology of invasive aspergillosis. aspergillus spondylodiscitis is rare in transplant recipients. the optimal treatment has yet to be defined because of the rarity of such cases. this article reviews the available literature on aspergillus spondylodiscitis in solid organ transplant recipients and provides recommendations on its management. | 2010 | 21168727 |
| the role of neuropeptide s and neuropeptide s receptor 1 in regulation of respiratory function in mice. | genome-wide screening and positional cloning have linked neuropeptide s receptor 1 (npsr1) with asthma and airway hyperresponsiveness. however, the mechanism by which npsr1 regulates pulmonary responses remains elusive. because neuropeptide s and its receptor npsr1 are expressed in brain regions that regulate respiratory rhythm, and npsr1-deficient mice have impaired stress and anxiety responses, we aimed to investigate whether neuropeptide s and npsr1 regulate respiratory function through a cen ... | 2010 | 21167892 |
| immune response to aspergillus fumigatus in compromised hosts: from bedside to bench. | the relevance of studies aimed at understanding host immune response against aspergillus fumigatus takes on much significance given that all patients with invasive aspergillosis are invariably immunocompromised. this article attempts to correlate relevant findings from recent experimental studies to clinical observations made by the physician at the bedside. it is hoped that the increased understanding of host-fungus immune interaction may pave the way for the development of new management strat ... | 2011 | 21162637 |
| role of everlasting triplet expansions in protein evolution. | analysis of occurrence of simple amino acid repeats in large ensemble of prokaryotic and eukaryotic sequences reveals that nearly all amino acids found in the repeats belong to those which have in their codon repertoires aggressively expanding triplets, all of three known pathologically expanding classes gcu (gcu, cug, ugc, agc, gca, cag), gcc (gcc, ccg, cgc, ggc, gcg, cgg), and aag (aag, aga, gaa, ctt, ttc, tct). this is observed especially clear in the first exons of proteins of higher eukaryo ... | 2010 | 21161200 |
| high attenuation mucoid impaction in allergic bronchopulmonary aspergillosis. | allergic bronchopulmonary aspergillosis (abpa) is a complex hypersensitivity syndrome triggered against antigens of aspergillus fumigatus, a fungus that most commonly colonizes the airways of patients with bronchial asthma and cystic fibrosis. it presents clinically with refractory asthma, hemoptysis and systemic manifestations including fever, malaise and weight loss. radiologically, it presents with central bronchiectasis and recurrent episodes of mucus plugging. the mucus plugs in abpa are ge ... | 2010 | 21160739 |
| aspergillus infection of supramid orbital implant and hyperostosis of orbital bone: report of a unique case. | the authors report the clinical findings in a patient who developed proptosis fourteen years after an orbital floor fracture repair with a supramid orbital implant due to hyperostosis of the orbital floor and lateral orbital wall bone secondary to aspergillus infection. | 2010 | 21158583 |
| aspergillus fumigatus meningitis in a preterm. | 2010 | 21149907 | |
| the tetraspanin cd82 is specifically recruited to fungal and bacterial phagosomes prior to acidification. | cd82 is a member of the tetraspanin superfamily, whose physiological role is best described in the context of cancer metastasis. however, cd82 also associates with components of the class ii major histocompatibility complex (mhc) antigen presentation pathway, including class ii mhc molecules and the peptide-loading machinery, as well as cd63, another tetraspanin, suggesting a role for cd82 in antigen presentation. here, we observe the dynamic rearrangement of cd82 after pathogen uptake by imagin ... | 2010 | 21149584 |
| aspergillus vertebral osteomyelitis in immunocompetent hosts: role of triazole antifungal therapy. | a review of published cases, in addition to a recently treated patient, is presented that describes the clinical features and outcomes of triazole therapy for vertebral aspergillosis in immunocompetent patients. | 2011 | 21148508 |
| echinocandin and triazole antifungal susceptibility profiles for candida spp., cryptococcus neoformans, and aspergillus fumigatus: application of new clsi clinical breakpoints and epidemiologic cutoff values to characterize resistance in the sentry antimicrobial surveillance program (2009). | the sentry antimicrobial surveillance program monitors global susceptibility and resistance rates for newer and established antifungal agents. the echinocandins displayed excellent potency against both candida spp. and aspergillus fumigatus in 2009 samples. using the new clinical and laboratory standards institute breakpoint values for the echinocandins when testing candida spp., we demonstrate low but measurable rates of resistance to all 3 echinocandins (anidulafungin, caspofungin, and micafun ... | 2011 | 21146713 |
| overexpression of the trichodiene synthase gene tri5 increases trichodermin production and antimicrobial activity in trichoderma brevicompactum. | trichoderma brevicompactum produces trichodermin, a simple trichothecene-type toxin that shares the first steps of the sesquiterpene biosynthetic pathway with other phytotoxic trichothecenes from fusarium spp. trichodiene synthase catalyses the conversion of farnesyl pyrophosphate to trichodiene and it is encoded by the tri5 gene that was cloned and analysed functionally by homologous overexpression in t. brevicompactum. tri5 expression was up-regulated in media with glucose, h(2)o(2) or glycero ... | 2010 | 21145409 |
| multiple-locus variable-number tandem repeat analysis for molecular typing of aspergillus fumigatus. | multiple-locus variable-number tandem repeat (vntr) analysis (mlva) is a prominent subtyping method to resolve closely related microbial isolates to provide information for establishing genetic patterns among isolates and to investigate disease outbreaks. the usefulness of mlva was recently demonstrated for the avian major pathogen chlamydophila psittaci. in the present study, we developed a similar method for another pathogen of birds: the filamentous fungus aspergillus fumigatus. | 2010 | 21143842 |
| [case of chronic necrotizing pulmonary aspergillosis complicated by elevated eosinophils and serum ige]. | a 76-year-old man who was being treated for bronchial asthma and pulmonary emphysema by his family physician experienced dyspnea and was referred to our department with suspected pneumonia. the patient responded poorly to sulbactam/cefoperazone and clarithromycin. a cavity lesion appeared in the left upper lobe, aspergillus was detected from his purulent sputum, and an aspergillus fumigatus-precipitating antibody was seen. therefore, chronic necrotizing pulmonary aspergillosis was diagnosed. blo ... | 2010 | 21141064 |
| low prevalence of resistance to azoles in aspergillus fumigatus in a french cohort of patients treated for haematological malignancies. | an increase in invasive aspergillosis (ia) due to azole-resistant aspergillus fumigatus isolates has been reported for 10 years. our study aimed to estimate the prevalence of azole resistance in isolates prospectively collected in patients with haematological diseases. | 2010 | 21131690 |
| identification of 3-phenylaminoquinolinium and 3-phenylaminopyridinium salts as new agents against opportunistic fungal pathogens. | previous studies on the indoloquinoline alkaloid, cryptolepine (2), revealed that it has antii-nfective properties among other activities. using structure-activity relationship (sar) techniques, several ring-opened analogs of cryptolepine (3-phenylaminopyridinium and 3-phenylaminoquinolinium derivatives) were designed to improve the potency and lower the cytotoxicity shown by several of the precursor agents. results indicate that these ring-opened analogs constitute new anti-infective agents wit ... | 2010 | 21130660 |
| expression of 5,8-lds of aspergillus fumigatus and its dioxygenase domain. a comparison with 7,8-lds, 10-dioxygenase, and cyclooxygenase. | 5,8-linoleate diol synthase (5,8-lds) of aspergillus fumigatus was cloned, expressed, and compared with 7,8-lds of the take-all fungus. replacements of tyr and cys in the conserved yrwh and fxxgphxclg sequences abolished 8r-dioxygenase (8-dox) and hydroperoxide isomerase activities, respectively. the predicted α-helices of lds were aligned with α-helices of cyclooxygenase-1 (cox-1) to identify the 8-dox domains. n-terminal expression constructs of 5,8- and 7,8-lds (674 of 1079, and 673 of 1165 r ... | 2010 | 21130068 |
| synthetic biotinylated tetra β(1→5) galactofuranoside for in vitro aspergillosis diagnosis. | the synthesis of a tetra β(1→5) galactofuranoside was achieved using a thioglycoside donor with a methyl tert-butyl phenyl thio leaving group. this tetrasaccharide was conjugated to biotin and validated as antigen with the monoclonal antibody used for clinical detection of aspergillus fumigatus galactomannan on streptavidin-coated microplates. then we have shown its ability to detect antibodies associated with a. fumigatus induced disease by using sera from patients with allergic broncho-pulmona ... | 2010 | 21129984 |
| pulmonary aspergillosis caused by a pan-azole-resistant aspergillus fumigatus in a 10-year-old boy. | a 10-year-old boy with high-risk acute lymphoblastic leukemia treated with hematopoietic stem-cell transplantation developed pulmonary aspergillosis while receiving prophylactic voriconazole. a transpleural aspirate culture revealed a pan-azole-resistant aspergillus fumigatus. treatment with liposomal amphotericin b resulted in complete recovery. as the frequency of azole resistance in a. fumigatus increases, invasive procedures to isolate fungi for species identification and susceptibility test ... | 2011 | 21127455 |
| β(1,3)-glucanosyl-transferase activity is essential for cell wall integrity and viability of schizosaccharomyces pombe. | the formation of the cell wall in schizosaccharomyces pombe requires the coordinated activity of enzymes involved in the biosynthesis and modification of β-glucans. the β(1,3)-glucan synthase complex synthesizes linear β(1,3)-glucans, which remain unorganized until they are cross-linked to other β(1,3)-glucans and other cell wall components. transferases of the gh72 family play important roles in cell wall assembly and its rearrangement in saccharomyces cerevisiae and aspergillus fumigatus. four ... | 2010 | 21124977 |
| use of epidemiological cutoff values to examine 9-year trends in susceptibility of aspergillus species to the triazoles. | in the absence of clinical breakpoints, epidemiological cutoff values (ecvs) have been established to distinguish wild-type (wt) isolates of aspergillus spp. from those that may harbor resistance mutations. recently, the clsi has developed ecvs for triazoles (itraconazole, posaconazole, and voriconazole) and common aspergillus species. we applied the triazole ecvs to 1,789 aspergillus isolates collected from 63 centers worldwide from 2001 to 2009 to determine the frequency of non-wt strains of e ... | 2010 | 21123534 |
| anti-infectious activity of intravitreal injectable voriconazole microspheres on experimental rabbit fungal endophthalmitis caused by aspergillus fumigatus. | the therapeutic effect of sustained intravitreal injectable voriconazole microspheres (vcz-ms) on experimental endophthalmitis caused by aspergillus fumigatus was investigated. vcz-ms were prepared and evaluated. vitrectomy was performed on rabbits after intravitreal inoculation of susceptible a. fumigatus. the animals were randomly divided into five groups, including control (no treatment after vitrectomy), vitrectomy plus voriconazole intravitreal injection, and vitrectomy plus intravitreal in ... | 2010 | 21108411 |
| antifungal activity of flavonoids isolated from mango (mangifera indica l.) leaves. | five flavonoids, namely (-)-epicatechin-3-o-β-glucopyranoside (1), 5-hydroxy-3-(4-hydroxylphenyl)pyrano[3,2-g]chromene-4(8h)-one (2), 6-(p-hydroxybenzyl)taxifolin-7-o-β-d-glucoside (tricuspid) (3), quercetin-3-o-α-glucopyranosyl-(1 → 2)-β-d-glucopyranoside (4) and (-)-epicatechin(2-(3,4-dihydroxyphenyl)-3,4-dihydro-2h-chromene-3,5,7-triol (5), were isolated from the leaves of mango (mangifera indica l.). antifungal activity of these compounds was evaluated against five fungal species, namely alt ... | 2010 | 21108117 |
| calcineurin-dependent galactomannan release in aspergillus fumigatus. | the galactomannan assay to diagnose invasive aspergillosis is recommended and clinically utilized, yet the mechanism of galactomannan release from aspergillus fumigatus is unknown. we used an a. fumigatus strain lacking calcineurin a (cnaa), already shown to be critically important for pathogenicity, to evaluate galactomannan kinetics. during the logarithmic growth phase when glucose was consumed, β-d-galactofuranoside (galf)-antigens were released in the culture. however, after glucose became l ... | 2010 | 21107627 |
| structure-function analysis of an enzymatic prenyl transfer reaction identifies a reaction chamber with modifiable specificity. | fungal indole prenyltransferases participate in a multitude of biosynthetic pathways. their ability to prenylate diverse substrates has attracted interest for potential use in chemoenzymatic synthesis. the fungal indole prenyltransferase ftmpt1 catalyzes the prenylation of brevianamide f in the biosynthesis of fumitremorgin-type alkaloids, which show diverse pharmacological activities and are promising candidates for the development of antitumor agents. here, we report crystal structures of unli ... | 2010 | 21105662 |
| fungal infections in icu patients: epidemiology and the role of diagnostics. | invasive fungal infections (ifis) are on the increase not only among oncology and transplant patients but also among patients admitted to intensive care units (icu). the rise in icu ifis can be attributed to the growing use of complex surgical procedures, invasive medical devices, and long-term, broad-spectrum antibiotic therapy. the majority of these life-threatening infections are caused by the well-known opportunistic pathogens candida albicans and aspergillus fumigatus, but new opportunistic ... | 2010 | 21102391 |
| does the use of biofuels affect respiratory health among male danish energy plant workers? | objectives to study asthma, respiratory symptoms and lung function among energy plant employees working with woodchip, straw or conventional fuel. methods respiratory symptoms in 138 woodchip workers, 94 straw workers and 107 control workers from 85 heating- or combined heating and power plants were collected by questionnaire. spirometry, metacholine provocation tests and skin prick tests were performed on 310 workers. the work area concentrations of 'total dust' (n=181), airborne endotoxin (n=1 ... | 2010 | 21098831 |
| [expression of integrin during the course of adhesion between fungi and corneal epithelial cells]. | to investigate the expression of integrin during the course of adhesion between fungi and corneal epithelial cells. | 2010 | 21092564 |
| [expression of inflammatory cytokines in a mouse model of keratomycosis]. | to investigate the expression, contribution and regulation of predominant inflammatory cytokines in the inflammation process of in a mouse model of keratomycosis. | 2010 | 21092562 |
| lipid signalling in pathogenic fungi. | in recent years, the study of lipid signalling networks has significantly increased. although best studied in mammalian cells, lipid signalling is now appreciated also in microbial cells, particularly in yeasts and moulds. for instance, microbial sphingolipids and their metabolizing enzymes play a key role in the regulation of fungal pathogenicity, especially in cryptococcus neoformans, through the modulation of different microbial pathways and virulence factors. another example is the quorum se ... | 2010 | 21091925 |
| protein expression profiles distinguish between experimental invasive pulmonary aspergillosis and pseudomonas pneumonia. | we hypothesized that invasive pulmonary aspergillosis (ipa) may generate a distinctive proteomic signature in plasma and bronchoalveolar lavage (bal). proteins in plasma and bal from two neutropenic rabbit models of ipa and pseudomonas pneumonia were analyzed by seldi-tof ms. hierarchical clustering analysis of plasma time course spectra demonstrated two clusters of peaks that were differentially regulated between ipa and pseudomonas pneumonia (57 and 34 peaks, respectively, p<0.001). pca of pla ... | 2010 | 21089047 |
| galactomannan testing in bronchoalveolar lavage fluid facilitates the diagnosis of invasive pulmonary aspergillosis in patients with hematologic malignancies and stem cell transplant recipients. | invasive pulmonary aspergillosis (ipa) is a major cause of mortality in patients with stem cell transplants and hematologic malignancies. timely diagnosis of ipa improves survival but is difficult to make. we evaluated the effectiveness of bronchoalveolar lavage (bal) galactomannan (gm) in diagnosing ipa in these populations by retrospectively reviewing records of 67 consecutive patients, in whom 89 bal gm tests were performed. for patients with ipa, only the first bal sample linked to the ipa e ... | 2010 | 21087680 |
| aspergillus fumigatus colonization in cystic fibrosis: implications for lung function? | clin microbiol infect abstract: aspergillus fumigatus is commonly found in the respiratory secretions of patients with cystic fibrosis (cf). although allergic bronchopulmonary aspergillosis (abpa) is associated with deterioration of lung function, the effects of a. fumigatus colonization on lung function in the absence of abpa are not clear. this study was performed in 259 adults and children with cf, without abpa. a. fumigatus colonization was defined as positivity of >50% of respiratory cultur ... | 2010 | 21087348 |
| exploring the bzip transcription factor regulatory network in neurospora crassa. | transcription factors (tfs) are key nodes of regulatory networks in eukaryotic organisms, including filamentous fungi such as neurospora crassa. the 178 predicted dna-binding tfs in n. crassa are distributed primarily among six gene families, which represent an ancient expansion in filamentous ascomycete genomes; 98 tf genes show detectable expression levels during vegetative growth of n. crassa, including 35 that show a significant difference in expression level between hyphae at the periphery ... | 2010 | 21081763 |
| human leukocytes kill aspergillus nidulans by reactive oxygen species-independent mechanisms. | invasive aspergillosis is a major threat for patients suffering from chronic granulomatous disease (cgd). although aspergillus fumigatus is the most commonly encountered aspergillus species, the presence of a. nidulans appears to be disproportionately high in cgd patients. the purpose of this study was to investigate the involvement of the nadph oxidase and the resulting reactive oxygen species (ros) in host defense against fungi and to clarify their relationship toward a. nidulans. murine cgd a ... | 2010 | 21078850 |
| comparative genomics and the evolution of pathogenicity in human pathogenic fungi. | because most fungi have evolved to be free-living in the environment and because the infections they cause are usually opportunistic in nature, it is often difficult to identify specific traits that contribute to fungal pathogenesis. in recent years, there has been a surge in the number of sequenced genomes of human fungal pathogens, and comparison of these sequences has proved to be an excellent resource for exploring commonalities and differences in how these species interact with their hosts. ... | 2010 | 21076011 |
| cleavage of resveratrol in fungi: characterization of the enzyme rco1 from ustilago maydis. | ustilago maydis, the causative agent of corn smut disease, contains two genes encoding members of the carotenoid cleavage oxygenase family, a group of enzymes that cleave double bonds in different substrates. one of them, cco1, was formerly identified as a β-carotene cleaving enzyme. here we elucidate the function of the protein encoded by the second gene, termed here as ustilago maydis resveratrol cleavage oxygenase 1 (um rco1). in vitro incubations of heterologously expressed and purified um r ... | 2010 | 21073977 |
| fungifun: a web-based application for functional categorization of fungal genes and proteins. | fungifun assigns functional annotations to fungal genes or proteins and performs gene set enrichment analysis. based on three different classification methods (funcat, go and kegg), fungifun categorizes genes and proteins for several fungal species on different levels of annotation detail. it is web-based and accessible to users without any programming skills. fungifun is the first tool offering gene set enrichment analysis including the funcat categorization. two biological datasets for aspergi ... | 2010 | 21073976 |
| ige, igga, iggb and igg(t) serum antibody levels in offspring of two sires affected with equine recurrent airway obstruction. | equine recurrent airway obstruction (rao) is a chronic lower airway disease of the horse caused by hypersensitivity reactions to inhaled stable dust, including mould spores such as aspergillus fumigatus. the goals of this study were to investigate whether total serum ige levels and allergen-specific ige and igg subclasses are influenced by genetic factors and/or rao and whether quantitative trait loci (qtl) could be identified for these parameters. the offspring of two rao-affected sires (s1: n= ... | 2010 | 21070286 |
| lack of standardization in the procedures for mycological examination of sputum samples from cf patients: a possible cause for variations in the prevalence of filamentous fungi. | filamentous fungi and yeasts are increasingly isolated from respiratory secretions of patients with cystic fibrosis (cf), and persistent fungal colonization of the airways of such patients is thought to exacerbate lung damage. while many independent studies have identified aspergillus fumigatus complex as the principal colonizing fungus in cf, increased awareness of the role of fungi in cf pathology coupled with improved mycological culture and identification methods have resulted in a number of ... | 2010 | 21067335 |
| serum markers for allergic bronchopulmonary aspergillosis in cystic fibrosis: state of the art and further challenges. | allergic bronchopulmonary aspergillosis (abpa), which results from hypersensitivity, primarily to aspergillus, represents a severe complication in patients suffering from asthma or cystic fibrosis (cf). since early treatment of abpa is supposed to prevent long-term damages, abpa has to be diagnosed promptly. however, this diagnosis is not straightforward due to clinical and radiological features of abpa overlapping with those of cf. despite abpa specific diagnosis criteria proposed by the cystic ... | 2010 | 21067334 |
| what can be learned from genotyping of fungi? | multiple genotyping studies have been carried out in order to clarify the epidemiology of fungal infections, more specifically to determine the sources, transmission routes, and colonization patterns of fungal isolates. in this review, the results obtained in genotyping investigations of aspergillus isolates are summarized and discussed. furthermore, we examine the epidemiologic studies of candida albicans, exophiala dermatitidis and scedosporium apiospermum infections in patients with cystic fi ... | 2010 | 21067332 |
| exophiala (wangiella) dermatitidis and cystic fibrosis - prevalence and risk factors. | the objective of this prospective study was to assess the prevalence of exophiala dermatitidis in respiratory secretions of patients with cystic fibrosis (cf) and to identify risk factors for its presence. the results of all cultures performed over a 2-year period in non lung-transplant patients in our cf clinic were included in the study. samples consisted of sputum (whenever possible) or deep pharyngeal aspirate after a session of physiotherapy. specimens were inoculated onto sabouraud gentami ... | 2010 | 21067329 |
| clinical associations and prevalence of scedosporium spp. in australian cystic fibrosis patients: identification of novel risk factors? | risk factors for the association of scedosporium in cases of cystic fibrosis (cf) and its clinical implications are poorly understood. clinical, lung function and laboratory data of adult cf patients in sydney (april 2008-march 2009) were prospectively analysed for such risk factors. expectorated sputa were cultured for bacteria and examined for fungi using standard mycological and scedosporium-selective media, and by an internal transcribed spacer region-targeted multiplex pcr assay. scedospori ... | 2010 | 21067328 |
| characteristics and consequences of airway colonization by filamentous fungi in 201 adult patients with cystic fibrosis in france. | a total of 657 sputum samples from 201 cystic fibrosis adult patients were collected during a 24-month period (2005-2006). we retrospectively analyzed the fungal colonization of the respiratory tract of these individuals by linking medical records and microbiological data. filamentous fungi were isolated from specimens of 65.6% of the patients, with aspergillus fumigatus being the predominant species recovered as it was found in specimens of 56.7% of the patients. we observed no difference for g ... | 2010 | 21067327 |
| fungal respiratory infections in cystic fibrosis: a growing problem. | 2010 | 21067320 | |
| toll like receptor 5 (tlr5) may be involved in the immunological response to aspergillus fumigatus in vitro. | toll-like receptors (tlrs) are involved in the host defense against aspergillus fumigatus infections, and some tlrs may even be exploited by the mould to escape immune mechanisms. we have previously shown that conidia from a. fumigatus increase expression of tlr5 in human monocytes. when further investigating a possible role of tlr5 in a. fumigatus infections, we observed a decrease in conidial viability after culturing with tlr5-knockdown thp-1 monocytes. secondly, our experiments showed an inc ... | 2010 | 21067314 |
| [comparison of glucan and galactomannan tests with real-time pcr for diagnosis of invasive aspergillosis in a neutropenic rat model.] | the incidence of aspergillosis which has high mortality rates, has increased gradually. since invasive aspergillosis (ia) is one of the leading causes of death in immunocompromized and neutropenic patients, early and accurate diagnosis of ia is of crucial importance. the aims of this study were to compare the results of culture, real-time polymerase chain reaction (rtpcr), galactomannan (gm) antigen and glucan (gc) antigen detection tests and to evaluate their performances in view of rapid and a ... | 2010 | 21063994 |
| aspergillus fumigatus acum regulates both iron acquisition and gluconeogenesis. | relatively few transcription factors that govern the virulence of aspergillus fumigatus are known. we constructed 11 a. fumigatus transcription factor mutants and screened them for altered virulence in galleria mellonella larvae. we discovered that the zinc cluster transcription factor, acum, is essential for maximal virulence in this model, as well as in murine models of haematogenously disseminated and invasive pulmonary aspergillosis. transcriptional profiling experiments suggested that acum ... | 2010 | 21062375 |
| tlr9 is actively recruited to aspergillus fumigatus phagosomes and requires the n-terminal proteolytic cleavage domain for proper intracellular trafficking. | tlr9 recognizes unmethylated cpg dna and induces innate immune responses. tlr9 activation is a multistep process requiring proteolytic cleavage and trafficking to endolysosomal compartments for ligand-induced signaling. however, the rules that govern the dynamic subcellular trafficking for tlr9 after pathogen uptake have not been established. in this study, we demonstrate that uptake of aspergillus fumigatus conidia induced drastic spatial redistribution of tlr9 to the phagosomal membrane of a. ... | 2010 | 21059889 |
| severe pneumonia caused by combined infection with pneumocystis jiroveci, parainfluenza virus type 3, cytomegalovirus, and aspergillus fumigatus in a patient with stevens-johnson syndrome/toxic epidermal necrolysis. | stevens-johnson syndrome (sjs) and toxic epidermal necrolysis (ten) are severe adverse cutaneous reactions to drugs. we report here the first case of severe pneumonia caused by an unusual combined infection with pneumocystis carinii (jiroveci), parainfluenza virus type 3, cytomegalovirus and aspergillus fumigatus in a 63-year-old female patient with allopurinol-induced sjs/ten overlap syndrome. following treatment with high-dose systemic corticosteroids and intravenous immunoglobulin for sjs/ten ... | 2010 | 21057748 |
| syphilis and the monk. | primary orbital aspergillus infection may occur in immunocompetent individuals. it frequently represents a diagnostic challenge for clinicians due to nonspecific clinical presentations and neuroimaging signs. we present a 47-year-old otherwise healthy man with an isolated unilateral optic neuropathy secondary to primary orbital aspergillosis. he had a remote history of tuberculosis and positive syphilis serologies. after he worsened despite intravenous penicillin therapy, a biopsy showed chronic ... | 2010 | 21056446 |
| endogenous fungal endophthalmitis after cardiac surgery. | the authors present a single-patient interventional case report of endogenous fungal endophthalmitis (efe) in a patient with an implanted prosthetic device. a 74-year-old man underwent a double coronary artery bypass graft with a bioprosthetic pericardial valve and transvenous dual chamber pacemaker. four months later, he presented with efe. despite aggressive local and systemic antifungal therapy, the patient died of septic shock due to a fungally infected pacemaker. successful management of po ... | 2010 | 21053864 |
| infectious complications in polymyositis and dermatomyositis: a series of 279 patients. | objectives:: to assess the prevalence and characteristics of severe pyogenic, nonpyogenic, and opportunistic infections in polymyositis and dermatomyositis (pm/dm) patients and to evaluate the predictive values for infections on clinical presentation and biochemical findings of pm/dm to detect patients at risk for such infections. methods:: the medical records of 279 consecutive pm/dm patients in 3 medical centers were reviewed. results:: one hundred four severe infections occurred in our patien ... | 2010 | 21047670 |
| aspergillus fumigatus allergen expression is coordinately regulated in response to hydrogen peroxide and cyclic amp. | abstract: | 2010 | 21047420 |
| [endophytic fungi from ginkgo biloba and their biological activities]. | to research the isolation method, identification and screen for bioactivities endophytic fungi from ginkgo. | 2010 | 21046747 |
| re-identification of aspergillus fumigatus sensu lato based on a new concept of species delimitation. | the species concept of aspergillus fumigatus sensu stricto has recently been defined by polyphasic taxonomy. based on the new concept of species delimitations, 146 worldwide strains of aspergillus fumigatus sensu lato were re-identified. of those 146 strains, 140 (95.8%) could be identified as a. fumigatus sensu stricto, 3 (2.1%) as a. lentulus, and the remaining 3 strains as a. viridinutans complex, neosartorya udagawae, and n. cf. nishimurae. of 98 clinical strains, only 1 from dolphin nostril ... | 2010 | 21046338 |
| acetazolamide-based fungal chitinase inhibitors. | chitin is an essential structural component of the fungal cell wall. chitinases are thought to be important for fungal cell wall remodelling, and inhibition of these enzymes has been proposed as a potential strategy for development of novel anti-fungals. the fungal pathogen aspergillus fumigatus possesses two distinct multi-gene chitinase families. here we explore acetazolamide as a chemical scaffold for the inhibition of an a. fumigatus 'plant-type' chitinase. a co-crystal structure of afchia1 ... | 2010 | 21044846 |
| the influence of β-glucan on the growth and cell wall architecture of aspergillus spp. | β-1,3-glucan is a major component of fungal cell walls with various biological activities, including effects on the production of inflammatory mediators in vivo and in vitro. however, few reports have examined its influence on the fungal cell itself. in this study, the influences of β-1,3-glucan on the growth and cell wall structure of fungi was examined. aspergillus fumigatus was cultured with a synthetic medium, c-limiting medium, in the presence or absence of β-1,3-glucan. hyphal growth was p ... | 2010 | 21044140 |
| coevolution of th1, th2, and th17 responses during repeated pulmonary exposure to aspergillus fumigatus conidia. | aspergillus fumigatus, a ubiquitous airborne fungus, can cause invasive infection in immunocompromised individuals but also triggers allergic bronchopulmonary aspergillosis in a subset of otherwise healthy individuals repeatedly exposed to the organism. this study addresses a critical gap in our understanding of the immunoregulation in response to repeated exposure to a. fumigatus conidia. c57bl/6 mice were challenged intranasally with a. fumigatus conidia weekly, and leukocyte composition, acti ... | 2010 | 21041495 |
| the tyrosine o-prenyltransferase sird catalyzes o-, n-, and c-prenylations. | recently, the prenyltransferase sird was found to be responsible for the o-prenylation of tyrosine in the biosynthesis of sirodesmin pl in leptosphaeria maculans. in this study, the behavior of sird towards phenylalanine/tyrosine and tryptophan derivatives was investigated. product formation has been observed with 12 of 19 phenylalanine/tyrosine derivatives. it was shown that the alanine structure attached to the benzene ring and an electron donor, e.g., oh or nh₂, at its para-position are essen ... | 2010 | 21038099 |
| aspergillus endocarditis: a review of the literature. | we present a case of cardiac device-related aspergillus endocarditis in a patient with a pacemaker and an allogeneic bone marrow transplant to segue into a review of the aspergillus endocarditis literature. aspergillus endocarditis should be suspected in patients with underlying immunosuppression, negative cultures, and a vegetation on echocardiography. diagnosis ultimately requires confirmation by tissue histology and culture. the optimal treatment approach often requires aggressive surgical de ... | 2010 | 21036091 |
| in vitro interactions among echinocandins against aspergillus fumigatus: lack of concordance among methods. | drug combinations are increasingly being explored as a means of improving outcomes in cases of invasive aspergillosis. however, the optimal methods for assessing in vitro drug combinations are unclear. we investigated whether echinocandin drug combinations have potentially useful interactions against aspergillus fumigatus. we explored the in vitro interactions of three echinocandins (caspofungin [csp], anidulafingin [anf], and micafungin [mcf]) by three methods, i.e., (i) checkerboard assay, (ii ... | 2010 | 21028945 |
| [not available]. | 1945 | 21008558 | |
| report of a case of aspergillus fumigatus infection of the tracheobronchial tree. | 1946 | 20997312 | |
| relations between the source of nitrogen and antibiotic formation by aspergillus fumigatus, fresenius. | 1946 | 20995495 | |
| the biological activity of a substance resembling gliotoxin produced by a strain of aspergillus fumigatus. | 1946 | 20995123 | |
| chemical composition and biological assays of essential oils of calamintha nepeta (l.) savi subsp. nepeta (lamiaceae). | aerial parts of wild calamintha nepeta (l.) savi subsp. nepeta growing spontaneously on the mediterranean coast (sardinia island, italy) and on the atlantic coast (portugal) were used as a matrix for the supercritical extraction of volatile oil with co(2). the collected extracts were analysed by gc-fid and gc-ms methods and their compositions were compared with that of the essential oil isolated by hydrodistillation, but the differences were not relevant. a strong chemical variability was observ ... | 2010 | 20981614 |
| comparison of the utility of five commercial kits for extraction of dna from aspergillus fumigatus spores. | the aim of this study was to compare the efficiency of dna extraction from water as well as from blood samples spiked with a. fumigatus spores, using selected commercial kits. extraction of dna according to manufacturer's protocols was preceded by blood cells lysis and disruption of fungal cells by enzymatic digestion or bead beating. the efficiency of dna extraction was measured by pcr using aspergillus-specific primers and sybr green i dye or taqman probes targeting 28s rrna gene. all methods ... | 2010 | 20978634 |
| microbiological analysis of a mummy from the archeological museum in zagreb. | in this paper we report the results of the microbiological analysis of the samples taken from the mummy from the collection of the archaeological museum in zagreb, croatia. samples were taken from specific places such as oral, orbital, abdominal cavity and bandages surrounding the mummy, and analyzed in department of microbiology and hospital infections in university hospital "dubrava" in zagreb and in national reference laboratory for systemic mycoses of croatian national institute of public he ... | 2010 | 20977065 |
| profiling the aspergillus fumigatus proteome in response to caspofungin. | the proteomic response of aspergillus fumigatus to caspofungin was evaluated by gel-free isobaric tagging for relative and absolute quantitation (itraq) as a means to determine potential biomarkers of drug action. a cell fractionation approach yielding 4 subcellular compartment fractions was used to enhance the resolution of proteins for proteomic analysis. using itraq, a total of 471 unique proteins were identified in soluble and cell wall/plasma membrane fractions at 24 and 48 h of growth in r ... | 2010 | 20974863 |
| what makes aspergillus fumigatus a successful pathogen? genes and molecules involved in invasive aspergillosis. | aspergillus fumigatus is an opportunistic pathogen that causes 90% of invasive aspergillosis (ia) due to aspergillus genus, with a 50-95% mortality rate. it has been postulated that certain virulence factors are characteristic of a. fumigatus, but the "non-classical" virulence factors seem to be highly variable. overall, published studies have demonstrated that the virulence of this fungus is multifactorial, associated with its structure, its capacity for growth and adaptation to stress conditio ... | 2010 | 20974273 |
| synthesis, molecular docking, and biological evaluation of novel triazole derivatives as antifungal agents. | twenty-eight novel triazole derivatives (compounds 1a-v, 2a-f) have been synthesized for structure-activity relationship studies as antifungal agents. the compounds were designed on the basis of the structure of fluconazole and molecular modeling of the active site of the cytochrome p450 14α-demethylase (cyp51). all of them are reported for the first time. their chemical structures are characterized by (1) h nmr, (13) c nmr, lc-ms, and elemental analysis. the antifungal activities have been eval ... | 2010 | 20973917 |
| aspergillus fumigatus cell wall components differentially modulate host tlr2 and tlr4 responses. | aspergillus fumigatus conidia attenuates host proinflammatory responses through modulation of toll-like receptor (tlr)2 and tlr4 signaling, but the precise mechanisms that mediate this effect are not known. in the present study, the role of the aspergillus cell wall polysaccharide constituents responsible for the modulation of host capability to mount a proinflammatory response was studied. aspergillus cell wall fractions and its major components showed differential capabilities in modulating ho ... | 2010 | 20971208 |
| fungal peritonitis among the peritoneal dialysis patients of four turkish centres. | this study evaluates the clinical findings and treatment of continuous ambulatory peritoneal dialysis (capd) patients with fungal peritonitis in istanbul from 2000 to 2010. the clinical records of 15 patients with fungal peritonitis among the total 795 patients were reviewed for the clinical and laboratory data. the mean duration of dialysis from the initiation of treatment until the development of fungal peritonitis was 41.14 months. fungal peritonitis was the primary episode of infection in ei ... | 2010 | 20969736 |
| allergic bronchopulmonary aspergillosis presenting with cavitary lesion and simulating a lung abscess. | a case of allergic bronchopulmonary aspergillosis (abpa) is being described in a 52-year-old female patient who presented with a cavitary lesion on skiagram chest and simulating a lung abscess. patient responded with the oral corticosteroid therapy with complete resolution of the initial radiographic abnormality. despite various radiological presentations described in the literature, a lung abscess like presentation in abpa is very rare and significant, because an early and correct diagnosis by ... | 2010 | 20966583 |
| abaa and weta govern distinct stages of aspergillus fumigatus development. | the opportunistic human pathogen aspergillus fumigatus produces a massive number of asexual spores (conidia) as the primary means of dispersal, survival, genome protection and infection of hosts. in this report, we investigate the functions of two developmental regulators, afuabaa and afuweta, in a. fumigatus. the afuabaa gene is predicted to encode an atts/tea dna-binding domain protein and is activated by afubrla during the middle stage of a. fumigatus asexual development (conidiation). the de ... | 2010 | 20966095 |
| pseudomonas aeruginosa and their small diffusible extracellular molecules inhibit aspergillus fumigatus biofilm formation. | aspergillus fumigatus is often isolated from the lungs of cystic fibrosis (cf) patients, but unlike in severely immunocompromised individuals, the mortality rates are low. this suggests that competition from bacteria within the cf lung may be inhibitory. the purpose of this study was to investigate how pseudomonas aeruginosa influences a. fumigatus conidial germination and biofilm formation. aspergillus fumigatus biofilm formation was inhibited by direct contact with p. aeruginosa, but had no ef ... | 2010 | 20964704 |
| aspergillus infections in birds: a review. | aspergillosis is an infectious, non-contagious fungal disease caused by species in the ubiquitous opportunistic saprophytic genus aspergillus, in particular aspergillus fumigatus. this mycosis was described many years ago, but continues to be a major cause of mortality in captive birds and, less frequently, in free-living birds. although aspergillosis is predominantly a disease of the respiratory tract, all organs can be involved, leading to a variety of manifestations ranging from acute to chro ... | 2010 | 20954008 |
| novel conformationally restricted triazole derivatives with potent antifungal activity. | in continuation of our work on azole antifungal agents, a series of new conformationally restricted triazole derivatives possessing benzylpiperidin-4-yl methyl amino side chains were designed and synthesized. all the new azoles showed moderate to excellent in vitro antifungal activity against most of the tested pathogenic fungi. several compounds (such as 12e, 12f, 12h and 12n) showed higher antifungal activity against candida albicans than fluconazole. moreover, compounds 12g-i also showed good ... | 2010 | 20950895 |
| degradation of fungal dna in formalin-fixed paraffin-embedded sinus fungal balls hampers reliable sequence-based identification of fungi. | identification of the etiologic agent responsible for sinus fungal ball (sfb) is rarely obtained due to either the culture of patient specimens not being ordered or if cultures were inoculated they proved to be negative. obviously, this has a significant impact on the design of appropriate therapeutic strategies. we investigated whether paraffin-embedded (pe) tissues, the only materials often available, were suitable for the correct identification of the responsible fungi. we obtained pe tissues ... | 2010 | 20950222 |
| pathogenicity of aspergillus fumigatus mutants assessed in galleria mellonella matches that in mice. | aspergillus fumigatus is a clinically important fungus with the ability to cause invasive aspergillosis with high mortality rates in immunocompromised patients and chronic pulmonary aspergillosis in immunocompetent individuals. virulence of mutants has traditionally been assessed using mammalian hosts such as mice and rats and more recently the fruit fly, drosophila melanogaster, demonstrated the potential to act as an in vivo host suitable for screening aspergillus mutants. in this study using ... | 2010 | 20950221 |
| articular aspergillosis of hip joints in turkeys. | aspergillosis is an important cause of morbidity and mortality in birds. turkey poults are known to be particularly susceptible to fungal infection. although the respiratory tract is the most commonly affected, dissemination can occur into virtually any organ. here, we report an unusual outbreak of articular aspergillosis in a flock of meat turkeys with clinical signs of lameness. between 7 and 11 weeks of age, turkeys had severe granulomatous osteoarthritis of the hip joints with necrosis of th ... | 2010 | 20945796 |
| augmenting the activity of antifungal agents against aspergilli using structural analogues of benzoic acid as chemosensitizing agents. | a number of benzoic acid analogues showed antifungal activity against strains of aspergillus flavus, aspergillus fumigatus and aspergillus terreus, causative agents of human aspergillosis, in in vitro bioassays. structure-activity analysis revealed that antifungal activities of benzoic and gallic acids were increased by addition of a methyl, methoxyl or chloro group at position 4 of the aromatic ring, or by esterification of the carboxylic acid with an alkyl group, respectively. thymol, a natura ... | 2010 | 20943191 |
| yield, content, and composition of peppermint and spearmints as a function of harvesting time and drying. | peppermint ( mentha × piperita l.) and spearmints ('scotch' spearmint, m. × gracilis sole, and 'native' spearmint, mentha spicata l.) are widely grown essential oil crops in more northern latitudes; however, there is limited information on how harvest time and drying influence peppermint and spearmint yield, oil composition, and bioactivity, when grown south of the 41st parallel. in this 2-year study, the effects of harvest time and drying on the yield, oil composition, and bioactivity of pepper ... | 2010 | 20942459 |
| hapx-mediated adaption to iron starvation is crucial for virulence of aspergillus fumigatus. | iron is essential for a wide range of cellular processes. here we show that the bzip-type regulator hapx is indispensable for the transcriptional remodeling required for adaption to iron starvation in the opportunistic fungal pathogen aspergillus fumigatus. hapx represses iron-dependent and mitochondrial-localized activities including respiration, tca cycle, amino acid metabolism, iron-sulfur-cluster and heme biosynthesis. in agreement with the impact on mitochondrial metabolism, hapx-deficiency ... | 2010 | 20941352 |
| [anti-infectious activity of intravitreal injectable voriconazole microspheres on experimental rabbit fungal endophthalmitis of aspergillus fumigatus]. | the therapeutic effect of sustained intravitreal injectable voriconazole microspheres (vcz-ms) on an experimental endophthalmitis of aspergillus fumigatus was investigated. vcz-ms was prepared successfully and its physico-chemical property was also evaluated. right eyes of albino rabbits were infected with an intravitreal injection of 1 000 cfu x ml(-1) of susceptible aspergillus fumigatus. all fungal endophthalmitis models were randomly divided into five groups 48 hours later: group a is contro ... | 2010 | 20939190 |