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resistance to beta-lactams among blood isolates of salmonella spp. in european hospitals: results from the sentry antimicrobial surveillance program 1997-98.the susceptibility to beta-lactams and the beta-lactamase content of 110 salmonella spp. blood isolates collected during 1997-98 in 19 european centers participating in the sentry surveillance program were studied. thirty-one isolates (28%) were resistant to penicillins, due to production of tem-1 (27 isolates), oxa-1 (three isolates) or tem-1 + oxa-1 (one isolate). all oxa-1 producers and 10 tem-1-producing isolates were also resistant to penicillin-clavulanic acid combinations. in the latter i ...200312588337
clinical microbiological case: acute osteomyelitis in a previously healthy child. 200312588333
[serotypes and antibiotic susceptibility of the genus salmonella in elche, spain].salmonellosis is one of the main public health problems in developed countries. the most common serotype is salmonella enteritidis, followed by s. typhimurium, s. hadar and s. virchow. each serotype's sensitivity to antibiotics is different, but in general, there has been an increase in the number of isolates resistant to antibiotics. this is especially true for s. hadar and nalidixic acid, although we have not isolated any strains resistant to ciprofloxacin. our study provides data that may hel ...200212582438
detection of salmonella enteritidis-specific immunoglobulin a antibodies in crop samples from chickens infected with salmonella enteritidis.the crop (ingluvies), an organ for food storage in most avian species when the proventriculus is full, is located at the base of the esophagus. little is known about any immunological capacity in the crop, and the current study was conducted to determine whether any antibodies to se could be found in crop flushes taken from white leghorn hens following infection with this organism. surprisingly, an exceptionally strong iga anti-se response could be detected in the crops of hens 17 d postchalleng ...200312580247
from the centers for disease control and prevention. outbreaks of salmonella serotype enteritidis infection associated with eating shell eggs--united states, 1999-2001. 200312578503
adjuvant effects of sugar cane extracts (sce) in chickens.the effects of sugar cane extracts (sce) on immune responses in chickens were studied. two- or 10-month-old chickens orally administered sce (500 mg/kg/day), for 3 consecutive days before immunized with sheep red blood cells, brucella abortus and salmonella enteritidis organisms, showed significantly increased and prolonged antibody responses to these antigens, compared to control chickens without sce. furthermore, chickens orally administered sce also revealed enhanced delayed type hypersensiti ...200312576716
incidence of salmonella from poultry products and their susceptibility to antimicrobial agents.the incidence of salmonella in 60 samples of poultry products of national origin available for consumers obtained from two local butcher shops and one canteen of the city of porto and the susceptibility to antimicrobial agents allowed for human or animal therapy were evaluated. the results show that poultry samples are frequently contaminated with salmonella (60%), belonging to 10 different serotypes. salmonella enteritidis and s. hadar were the most prevalent serotypes. in addition, a high numb ...200312568749
enteric fever in mumbai, india: the good news and the bad news. 200312567317
persistence of salmonella enteritidis phage type 4 in the environment and arthropod vectors on an empty free-range chicken farm.the persistence of s. enteritidis pt4 was studied on a free-range breeding chicken farm which had been depopulated following identification of the organism in breeding birds. the site was sampled periodically for 26 months after depopulation and the organism was found to persist in litter, dried faeces and feed, but not in dust within empty poultry houses, for the whole of that period. salmonella enteritidis pt4 was also found in soil samples after 8 months but not 13 months and in faeces from w ...200312558590
identification and quantification of granulocytes in caecal mucosa and submucosa of chickens experimentally infected with eimeria tenella and salmonella enteritidis.1. poultry granulocytes are not clearly distinguished from each other with haematoxylin-eosin (he) stain; thus, histochemical techniques must be used. three experiments were carried out using 4-week-old leghorn chickens. 2. three, 80-chicken groups were orally infected with (1) 10(8) colony forming units (cfus) salmonella enteritidis, or (2) 10(4) eimeria tenella oocysts, or (3) 10(8) cfus s. enteritidis + 10(4) e. tenella oocysts. ten chickens from each group were euthanased and caecum samples ...200212555889
t lymphocyte subpopulations and b lymphocyte cells in caecum and spleen of chicks infected with salmonella enteritidis.the effect of low and high doses of salmonella enteritidis pt4 (se) on immunocompetent cells in caecum and spleen of one-day-old chicks was investigated. subsets of t lymphocytes positive for cd3, cd4, cd8 and b lymphocytes (bu1b-positive cells) were counted in the caecum after immunohistochemical staining and the relative percentage of these cells in the spleen was analysed using a facscan cytometer on days 7, 10, 14, 21, and 27 post-inoculation (pi). in the low dose group, the number of cd3+ a ...200212553717
outbreaks of salmonella serotype enteritidis infection associated with eating shell eggs--united states, 1999-2001.a salmonella serotype enteritidis (se) epidemic emerged in the 1980s, when increasing numbers of infections were detected in the northeastern and mid-atlantic regions of the united states. in the early 1990s, while se rates in the northeast began to decline, the se epidemic expanded to the pacific region. nationwide, the number of se isolates reported to cdc peaked at 3.8 per 100,000 population in 1995. although rates of culture-confirmed se infection reported to cdc declined to 1.9 by 1999 (fig ...200312553566
characterization of class i integrons in clinical strains of salmonella enterica subsp. enterica serovars typhimurium and enteritidis from norwegian hospitals.the characterization of integrons and their promoters in 156 antibiotic-resistant clinical isolates of the important zoonotic pathogens salmonella enterica subsp. enterica serovars typhimurium (s. typhimurium) and enteritidis (s. enteritidis) from norwegian hospitals were performed. integrons were found in 64 of 66 s. typhimurium isolates (97 %) and in 20 of 90 s. enteritidis isolates (22.2 %) with the following sizes; 650, 1000, 1200, 1500, 1600, 1700, 2000 and 2100 bp. the integrons were furth ...200312543920
investigation of salmonella contamination and disinfection in farm egg-packing plants.as part of a field-based study of the distribution and persistence of salmonella infection on commercial egg-laying farms, sampling was carried out on one or more occasions in egg-packing areas of 12 farms infected with salm. enteritidis.200312534810
protection against murine listeriosis by oral vaccination with recombinant salmonella expressing protective listerial epitopes within a surface-exposed loop of the tolc-protein.based on the topology of the outer membrane protein tolc of escherichia coli, a new plasmid-encoded system was created which allows the expression of antigenic peptides within permissive, surface-exposed domains of tolc. to assess the capacity of this novel antigen display system, a protective cd4 t-cell epitope of the p60 protein of listeria monocytogenes was inserted within an extracellular loop of the tolc-protein and expressed in surface-exposed form by attenuated salmonella enteritidis. mic ...200312531354
use of cecal microflora cultured under aerobic or anaerobic conditions in the control of experimental infection of chicks with salmonella enteritidis.one-day-old broiler chicks received cecal microflora (cm) cultured under aerobic, anaerobic conditions, or both (mixed) and were then infected with salmonella enteritidis, in order to compare the efficacy of these different types of culture in terms of the number of chicks infected, cecal colonization and faecal excretion of the challenging bacteria. regardless of culture type, cm always led to a smaller number of s. enteritidis for any of the parameters studied compared to untreated chicks. aer ...200312523985
inactivation of salmonella enterica serovar enteritidis by ultrasonic waves under pressure at different water activities.the inactivation of salmonella enterica serovar enteritidis by ultrasonic waves (20 khz; 117- microm wavelength) under pressure (175 kpa) at nonlethal temperatures (manosonication [ms]) and lethal temperatures (manothermosonication [mts]) in media of different water activities has been investigated. heat decimal reduction time values increased 30 times when the water activity was decreased from nearly 1 to 0.96, but the ms resistance was increased only twofold. the inactivation of salmonella ser ...200312514058
high ph during trisodium phosphate treatment causes membrane damage and destruction of salmonella enterica serovar enteritidis.trisodium phosphate (tsp) is now widely used during the processing of poultry and red meats, but the mechanism whereby it inactivates gram-negative bacteria such salmonella spp. remains unclear. thus, salmonella enterica serovar enteritidis (atcc 4931) cells were treated with different concentrations of tsp (1.5, 2.0, and 2.5% [wt/vol]) and compared with (i) cells treated with the same ph as the tsp treatments (ph 10.0, 10.5, and 11.0, respectively) and (ii) cells treated with different concentr ...200312513986
a questionnaire-based, retrospective field study of persistence of salmonella enteritidis and salmonella typhimurium in danish broiler houses.a questionnaire-based, retrospective field study was conducted in 78 danish broiler houses (analytical units) on 42 farms. in spring 1997, all these broiler houses had been infected with salmonella enteritidis, phage type 8, and/or salmonella typhimurium, definitive-type 66, by day-old chicks delivered from the same hatchery. because these two salmonella types had not been detected in the danish broiler sector before and the broiler houses were infected in a period of low salmonella prevalence, ...200312507854
effects of live attenuated and killed salmonella vaccine on t-lymphocyte mediated immunity in laying hens.the impact of live and killed salmonella vaccines on cell-mediated immunity (cmi) was investigated in 18- and 32-week-old white leghorn chickens, by assessing splenic lymphocyte proliferation, expression of il-2 mrna in concanavalin a (con a) stimulated cells and flow cytometric analysis of cell subpopulations. con a and salmonella enteritidis (se) flagella induced proliferation of splenocytes were enhanced in the 18- and 32-week-old chickens treated with live vaccine, compared to the correspond ...200312507848
inactivation of salmonella enteritidis during boiling of eggs.a series of inactivation curves for salmonella enteritidis were determined for boiling eggs using different conditions of time and temperature. no significant influence of egg weight could be found on the temperature evolution in the yolk. the inactivation curves consistently showed an initial slow decline in bacterial number at lower temperatures, after which a very rapid inactivation took place. it was not possible to reproduce this behavior using a traditional inactivation model. a pragmatic ...200312505456
eggshell matrix proteins as defense mechanism of avian eggs.this study focused on the role of eggshell matrix proteins as a function of potential natural antimicrobial defenses of avian eggs. the electrophoretic profile of sds-page showed that the soluble eggshell matrix proteins had three major bands of 15 000, 36 000, and 66 000 and several minor bands comprising 17 000, 25 000, 30 000, and 75 000, while insoluble matrix proteins were consisting of various bands comprising at least 16 distinct migration bands between 10 000 and 200 000. three bacteria ...200312502416
impaired accumulation and function of memory cd4 t cells in human il-12 receptor beta 1 deficiency.defects in il-12 production or il-12 responsiveness result in a vulnerability to infection with non-viral intracellular organisms, but the immunological mechanisms responsible for this susceptibility remain poorly understood. we present an immunological analysis of a patient with disseminated salmonella enteritidis and a homozygous splice acceptor mutation in the il-12rbeta1-chain gene. this mutation resulted in the absence of il-12rbeta1 protein on pbmc and an inability of t cells to specifical ...200312496448
mucosal humoral immunity to experimental salmonella enteritidis infection in the chicken crop.in this report, we show that chickens, infected with salmonella enteritidis (se) by oral gavage, produce secretory immunoglobulin as (sigas) that specifically bind to numerous se antigens. chickens infected with se showed strong siga response against flagella in both bile and crop. the optical density values of enzyme-linked immunosorbent assay (elisa) tests in positive bile and crop were 1.17 and 0.38, respectively, and were significantly different from those of negative samples. western immuno ...200212495067
associations of interferon-gamma genotype and protein level with antibody response kinetics in chickens.although previous studies have demonstrated an association between interferon-gamma (ifn-gamma) promoter genotype and antibody response kinetics in chickens, the protein levels that may mediate such a gene-trait association have not been determined. the objective of this study, therefore, was to determine the correlation of circulating ifn-gamma levels with both the ifn-gammaifn-gamma promoter polymorphisms and antibody response in order to evaluate the potential role of ifn-gamma protein in med ...200212495047
[salmonella enteritis in dogs, not relevant?].in order to shed some light on the different opinions regarding the importance of salmonella infections in dogs, we gathered some data from our laboratory database. of the 6589 faecal samples from diarrhoeic dogs examined, 69 (1%) yielded salmonella spp. another eleven isolates were cultured from materials other than faeces. if salmonella spp. can be cultured directly from faeces, this should be interpreted as clinically significant; however, even if the organism is found only after enrichment i ...200212491970
lack of flagella disadvantages salmonella enterica serovar enteritidis during the early stages of infection in the rat.the roles of flagella and five fimbriae (sef14, sef17, sef21, pef, lpf) in the early stages (up to 3 days) of salmonella enterica serovar enteritidis (s. enteritidis) infection have been investigated in the rat. wild-type strains la5 and s1400 (fim+/fla+) and insertionally inactivated mutants unable to express the five fimbriae (fim-/fla+), flagella (fim+/fla-) or fimbriae and flagella (fim-/fla-) were used. all wild-type and mutant strains were able to colonize the gut and spread to the mesente ...200312488571
spontaneous bacterial peritonitis in patients with hepatic cirrhosis: evaluation of a treatment protocol at specialized units.spontaneous bacterial peritonitis is a common and severe complication in patients with cirrhosis and ascitis. its prognosis clearly depends on its precocious clinical recognition and efficacious therapy.200212486852
green-leaf-derived c6-aroma compounds with potent antibacterial action that act on both gram-negative and gram-positive bacteria.all eight c6-aliphatic alcohol and aldehyde compounds in naturally occurring green leaves showed bacteriostatic effects against staphylococcus aureus ifo 12732, methicillin-resistant s. aureus, escherichia coli ifo 3301, e. coli o157:h7, and salmonella enteritidis, with bacteriostatic activities of less than 12.5 microg ml(-1). in this study, the susceptibility of gram-positive bacteria tested was observed to be greater than that of gram-negative bacteria. the bactericidal action of the aldehyde ...200212475283
effects of intraperitoneal and intranasal application of lentinan on cellular response in rats.lentinan (ajinomoto, japan) was administrated intraperitoneally (i.p.) and intranasally (i.n.) at different doses (1, 5 and 10 mg/kg) to rats. effectiveness of lentinan treatment was evaluated by comparative testing of cell activation (establishing the number, glycolytic and acid phosphatase activity, h2o2 production and killing ability against salmonella enteritidis and staphylococcus aureus) at two different compartments--peritoneal and broncho-alveolar cavities. the results indicated that len ...200212469938
fatal salmonella aortitis with mycotic aneurysm rupture.salmonellae most commonly cause uncomplicated cases of gastroenteritis but have a predilection for damaged blood vessels, especially those damaged by atherosclerosis. the abdominal aorta is most frequently affected. the most serious complication of aortitis is mycotic aneurysm formation with subsequent rupture. the authors present the case of a 61-year-old man who was found unresponsive at home 3 days after discharge from the hospital for treatment of gastroenteritis with bacteremia. postmortem ...200212464818
microsatellite markers associated with quantitative trait loci controlling antibody response to escherichia coli and salmonella enteritidis in young broilers.a unique resource population was produced to facilitate detection of microsatellite markers associated with quantitative trait loci controlling antibody (ab) response in broiler chickens. three f1 males were produced by mating two lines divergently selected on ab response to escherichia coli vaccination. each f1 male was mated with females from four genetic backgrounds: f1, high-ab line (hh), low-ab line and commercial line, producing three resource families, each with four progeny types. about ...200212464014
results of a 5-year prospective surveillance study of antibiotic resistance among salmonella enterica isolates and ceftriaxone therapy among children hospitalized for acute diarrhea.the spread of resistant salmonella strains continues to increase worldwide. it is necessary to establish epidemiologic information to determine an appropriate empiric antibiotic regimen (when indicated) in infants and children with suspected salmonella infections for whom the results of susceptibility tests are not yet available.200212462288
identification of possible chicken intestinal mucosa receptors for sef21-fimbriated salmonella enterica serovar enteritidis.in order to test whether glycosphingolipids (gsls) on the chicken intestinal mucosa serve as a receptor for salmonella enterica serovar enteritidis with fimbriae, we analyzed neutral gsls and gangliosides from chicken intestinal mucosa and investigated the binding of bacteria to neutral gsls and gangliosides. four kinds of neutral gsls, designated as n-1 to n-4 and four kinds of gangliosides, named g-1 to g-4, were identified on high-performance thin-layer chromatography (hptlc) plates. in tlc i ...200312458170
entry and survival of salmonella enterica serotype enteritidis pt4 in chicken macrophage and lymphocyte cell lines.various leukocytes are involved in the reaction to counter salmonella infection in chicken. the various leukocyte types react differently after an infection, since some clear the infection while others may cause dissemination of salmonella throughout the chicken. therefore, we investigated in vitro the entry and survival of salmonella enterica serotype enteritidis in chicken cell lines of various cell types, including two macrophage cell lines, hd11 and mq-ncsu (ncsu), two b-cell lines lscc-1104 ...200312458164
occurrence of salmonellae in retail chicken carcasses and their products in spain.this study examined the incidence of salmonella in spanish poultry products. samples included chicken carcasses, chicken parts (wings, legs and giblets-livers and hearts) and processed chicken products (red sausages, white sausages and hamburgers). the average detection rate was 49%, with the highest (55%) in chicken carcasses (skin) and the lowest (20%) in hamburgers. the chicken carcasses purchased in supermarkets were more contaminated (75%) than those from poulterers shops (25%). salmonella ...200312457592
evaluation of aviguard, a commercial competitive exclusion product for efficacy and after-effect on the antibody response of chicks to salmonella.the competitive exclusion (ce) action of aviguard (ag) and its effects on the antibody response of chicks were evaluated in this study. we observed that ag protected the chicks from overwhelming colonization. fourteen days after infection, fewer ag-pretreated than nonpretreated chicks shed salmonellae from their coloaca in both infected groups, although much less from se-infected chicks. antibody titers of sera produced to salmonella typhimurium (st) and se in pretreated and non-pretreated chick ...200212455592
pet reptiles associated with a case of salmonellosis in an infant were carrying multiple strains of salmonella. 200212454202
[intestinal infections]. 200212451635
influence of enrofloxacin and chloramphenicol on the level of igy in serum and egg yolk after immunostimulation of hens with salmonella enteritidis antigens.in the chicken, maternal antibodies are transferred into the egg and subsequently transported into the developing embryo. igg (called igy) is the primary immunoglobulin isotype of the egg yolk. their level in serum depends on the correct function of immunological system in laying hens. many factors have a direct or indirect influence on antibody level in fowl. one of them is a commonly used antibiotic, but its influence on avian immune system is still unknown. the objective of the study was to d ...200212448078
multidrug-resistant salmonella typhimurium and salmonella enteritidis identified by multiplex pcr from animals.antibiotic resistance in salmonella enteritidis and s. typhimurium, one of most frequent etiologic pathogens of food-borne bacterial gastroenteritidis in humans, is a serious health problem worldwide. fifteen and 22 each of s. enteritidis and s. typhimurium were isolated from animals from 1983 to 1999 in korea and tested for their antibiotic resistance patterns and phage types. s. enteritides isolates were highly resistant to sulfonamides (86.7%) and four of them (26.6%) showed multiple antibiot ...200112441686
association of mhc class i and class ii gene polymorphisms with vaccine or challenge response to salmonella enteritidis in young chicks.salmonella enteritidis (se) is a worldwide source of salmonellosis in humans, caused mainly by consumption of contaminated poultry products. the major histocompatibility complex (mhc) class i and class ii molecules, which function as antigen-presentation structures, are involved in both macrophage and dendritic cell immune response to salmonella and may, therefore, play an important role in host resistance to infections. the chicken mhc class i and class ii were investigated as candidate genes f ...200212439621
magnetized carbonyl iron and insoluble zirconium hydroxide mixture facilitates bacterial concentration and separation from nonfat dry milk.a mixture of magnetized carbonyl iron and insoluble zirconium hydroxide was investigated for its ability to concentrate various foodborne pathogens from 25-ml samples of reconstituted nonfat dry milk. each sample was artificially contaminated with 10(3) to 10(6) cfu/25 ml of representative foodborne pathogens (salmonella enterica serovar enteritidis, listeria monocytogenes, and bacillus cereus spores) and processed for bacterial concentration with high-speed centrifugation for the primary concen ...200212430708
[primary septic arthritis of the knee caused by salmonella enteritidis in systemic lupus erythematosus]. 200212426852
dietary mannan-oligosaccharides and their effect on chicken caecal microflora in relation to salmonella enteritidis colonization.this study first investigates the effects of mash diet, or mash supplemented with either 2.5% mannose-oligosaccharide (mos) or palm kernel meal (pkm), on the microflora of the hen caecal contents. second, it investigates the effect of caecal contents of hens (hcc) fed mash or mash supplemented with mos or pkm on the major microflora groups of chicks, and their inhibitory effect on salmonella enterica serovar enteritidis (pt4) colonization. finally, this study investigates the effect over time of ...200212425792
quantitative comparison of intestinal invasion of zoonotic serotypes of salmonella enterica in poultry.the aim of the present study was to compare the invasion of selected zoonotic salmonella serotypes of poultry in an in vivo chicken intestinal loop model and also in vitro in epithelial cell cultures. invasion was measured relative to a reference strain, salmonella typhimurium 4/74 invh201::tnphoa. two serotypes demonstrated intracellular log(10) counts that differed significantly from all other serotypes tested: salmonella enteritidis pt4 being 1.5 log(10) colony forming units (cfu) (31-fold) h ...200212425791
salmonella enteritidis pericarditis: case report and review of the literature.a 65-year-old male patient with primary idiopathic dermatomyositis and on regular immunosuppressive therapy was admitted to our department for fever and dyspnea. a diagnosis of hemorrhagic salmonella enteritidis pericarditis was made. treatment necessitated aggressive medical/surgical supervision. a review of the pertinent literature revealed that during the last decade salmonella enteritidis has been identified as the most frequent agent of pericarditis caused by salmonella species apart from s ...200212402668
[erythema nodosum linked to salmonella enteritidis in two brothers]. 200212396952
an evaluation of the risk assessment method used by environmental health officers when inspecting food businesses.two outbreaks of infection with salmonella enteritidis phage types 5c and 6a occurred in a number of scottish health board areas between may 2000 and january 2001. a case-control study of food businesses was subsequently carried out to ascertain whether the scores derived from environmental health officers' inspections prior to the outbreaks differed between food businesses where outbreak cases had eaten in the week before the onset of their illness (case food businesses) and neighbouring food b ...200212396525
serological monitoring of 40 swiss fancy breed poultry flocks.rapid serum agglutination, haemagglutination inhibition and enzyme-linked immunosorbent assays were used to screen swiss fancy breed chicken flocks for antibodies against 12 avian infectious agents. for this purpose, 1,002 blood samples from 40 flocks were collected and tested. ten percent of the samples were positive for salmonella gallinarum-pullorum and 62.5% of the flocks were affected. more than 75% of the flocks had antibodies against mycoplasma gallisepticum/mycoplasma synoviae, infectiou ...200212396360
the efficacy of salenvac, a salmonella enterica subsp. enterica serotype enteritidis iron-restricted bacterin vaccine, in laying chickens.the protective effect of two vaccination regimes using salenvac, a commercially available iron-restricted salmonella enterica subsp. enterica serotype enteritidis pt4 bacterin vaccine, was verified in laying birds. immunization was intramuscular at 1 day old and again at 4 weeks of age (v2), or at 1 day and 4 weeks with a third dose at 18 weeks of age (v3). challenge s. enteritidis (5 to 7.5); x 10(7) colony forming units) was given intravenously at 8, 17, 23, 30 and 59 weeks of age. for all age ...200212396340
[salmonella enteritidis abdominal aortic aneurysm in an hiv-infected patient]. 200112385057
mycotic aneurysm presenting as acute pyelonephritis.this report describes a 56-year-old man with a ruptured infected abdominal aortic aneurysm secondary to salmonella bacteremia, initially presenting as acute pyelonephritis. spike fever with severe back pain continued despite empiric antibiotic treatment at a local hospital. hypotension with a sudden hemoglobin drop was observed on the second hospitalization day. abdominal computed tomography to further examine the bleeding focus confirmed a rupture of the mycotic abdominal aortic aneurysm. this ...200212380916
identification of salmonella enteritidis isolates by polymerase chain reaction and multiplex polymerase chain reaction.the polymerase chain reaction and the multiplex polymerase chain reaction were developed for detection of salmonella and for identification of the serotype enteritidis. three sets of primers were selected from different genomic sequences amplifying a 429 bp fragment specific for the genus salmonella within a randomly cloned sequence, including a 250 bp fragment within the spv gene, and a 310 bp fragment within the sefa gene specific for salmonella enteritidis. the polymerase chain reaction and t ...200212380786
differential effects of age on chicken heterophil functional activation by recombinant chicken interleukin-2.interleukin-2 (il-2) exercises an array of biological effects on many cells including the functional activation of cells of the innate immune response. heterophils, the avian equivalent of the neutrophil, function as professional phagocytes to aid in regulation of innate host defenses. the objective of the present studies was to examine the effects of recombinant chicken il-2 (rchil-2) on functional activities of heterophils from chickens during the first 3 weeks after hatch. peripheral blood he ...200212377221
[salmonellosis in poland in 2000].in 2000, 22,799 cases of salmonellosis were reported to the sanitary epidemiological stations, incidence rate = 59.0 per 100,000 population. above 65% of patients were hospitalized. the seasonal peak was noted in may and june. most of cases (70%) were laboratory confirmed by isolation salmonella strains types. salmonella enteriditis was the most frequent type: 91% of cases and 70% of infected healthy persons. other serotypes--typhimurium, infantis, hadar and virchow, caused 5% salmonella cases o ...200212371361
isolation of antimicrobial compounds from guava (psidium guajava l.) and their structural elucidation.four antibacterial compounds were isolated from leaves of guava (psidium guajava l.), and the structures of these compounds were established on the basis of chemical and spectroscopic evidence. two new flavonoid glycosides, morin-3-o-alpha-l-lyxopyranoside and morin-3-o-alpha-l-arabopyranoside, and two known flavonoids, guaijavarin and quercetin, were identified. the minimum inhibition concentration of morin-3-o-alpha-l-lyxopyranoside and morin-3-o-alpha-l-arabopyranoside was 200 microg/ml for e ...200212353635
plasmid pc present in salmonella enterica serovar enteritidis pt14b strains encodes a restriction modification system.salmonella enterica serovar enteritidis (s. enteritidis) possesses plasmids of different sizes and roles. besides the serovar-specific virulence plasmid present in most field strains, s. enteritidis can harbour plasmids of low molecular mass whose biological role is poorly understood. we therefore sequenced plasmid pc present in s. enteritidis strains belonging to phage type pt14b. the size of plasmid was determined to be 5,269 bp and it was predicted to encode four open reading frames (orfs). t ...200212351230
multiresistant clones of salmonella enterica: the importance of dissemination.increasing anitmicrobial resistance among foodborne pathogens has prompted calls for the reduction of anitmicrobial use in livestock to prevent future emergence or resistance. in the case of salmonella enterica, clonal dissemination may play a more critical role in regional changes in antimicrobial resistance in salmonellae than antimicrobial selection pressure. multi-resistant salmonella typhimurium definitive type 104 (mr-dt104) emerged from an unknown location and was disseminated globally du ...200212271270
comparison of the effects of infection with salmonella enteritidis, in combination with an induced molt, on serum levels of the acute phase protein, alpha1 acid glycoprotein, in hens.periods of inflammation due to infection, injury, or malignancy are marked by increases in serum constituents known as acute phase proteins (app), and these proteins have been used as markers for early stages of disease. four experiments were performed to examine whether levels in chickens of one such app, alpha1 acid glycoprotein (agp), would be affected by an infection with salmonella enteritidis (se) and if the added stress of induced molting via 14-d feed withdrawal would increase these effe ...200212269607
[does emergency tropical medicine exist? the physician's point of view].the existence of tropical medical emergencies is a recurrent issue that joins the debate over the definition of tropical medicine. is it medicine practiced in warmer climates, medicine practiced with poor diagnostic and therapeutic facilities or medicine involving only tropical diseases? presentation of a few case reports provides a better response to this question than a long speech. the first case involves a 57-year-old man presenting a complicated attack of plasmodium falciparum malaria and s ...200212244920
risk factors for sporadic infection with salmonella enteritidis, denmark, 1997-1999.in a prospective case-control study of sporadic salmonella enteritidis infection in denmark (1997-1999), foreign travel was reported by 25% of 455 case patients and 8% of 507 controls (odds ratio (or) = 3.7, 95% confidence interval (ci): 2.4, 5.5). among nontravelers, 80% of 335 cases and 81% of 467 controls had consumed eggs or dishes containing raw or undercooked eggs during the week before disease onset or interview, while 35% of cases and 19% of controls had incurred this exposure the day be ...200212244034
a laboratory study of an inactivated bivalent iron restricted salmonella enterica serovars enteritidis and typhimurium dual vaccine against typhimurium challenge in chickens.a commercial inactivated iron restricted salmonella typhimurium and salmonella enteritidis vaccine was used to vaccinate chicks at 1 day and again at 4 weeks of age, with challenge by a high and a low dose of s. typhimurium given either orally or by contact with seeder birds inoculated orally with a high dose of s. typhimurium. in all three challenge regimes, the shedding of challenge strain was reduced significantly (p < 0.05) in vaccinated birds compared with unvaccinated controls. vaccination ...200212243894
characteristics of salmonella enteritidis contamination in eggs after oral, aerosol, and intravenous inoculation of laying hens.experimental infection models are useful tools for understanding how salmonella enteritidis is deposited in eggs and for testing potential strategies to control eggborne transmission of disease to humans. oral inoculation of laying hens is presumed to provide the closest simulation of naturally occurring infections, but alternatives such as intravenous or aerosol inoculation have sometimes been recommended as options to induce higher incidences of egg contamination. the present study compared th ...200212243527
resistance to velogenic newcastle disease virus in leghorn chickens by use of prophylactic lymphokines.a group of 1-day-old commercial leghorn chickens was prophylactically treated with lymphokines obtained from lymphocyte cultures of chickens previously infected with salmonella enteritidis (s. enteritidis-immune lymphokines [se-ilk]) with the objective to investigate the effect of se-ilk on development of newcastle disease (nd) infection caused by chimalguacan strain, a mexican velogenic nd virus (vndv). clinical signs, histologic lesions, and hemagglutination-inhibition (hi) serum titers were c ...200212243514
agglutination assays and elisa for detecting egg yolk antibodies in flocks naturally infected with salmonella enteritidis. 200212243276
[influence of the conditions of storage and cooking on growth, invasion and survival of salmonella enteritidis in eggs].basal studies for the confirmation of sanitary rules in the kitchen were performed, focusing on preventing an outbreak of food poisoning due to eggs contaminated with salmonella enteritidis (se), using hen and quail eggs. se did not grow at 5 degrees c but grew markedly at 25 degrees c in eggs. the invasion and growth of se were marked under very humid conditions regardless of whether the eggshell was damaged. the invasion of se into egg also occurred when eggs were taken in and out of the refri ...200212238158
[gonarthritis due to salmonella enteritidis in a patient with systemic lupus erythematosus].extraintestinal manifestations of salmonella infection occur more frequently in immunocompromised patients than in healthy persons. about 25 % present as septic arthritis. particularly patients with sle are predisposed. we report a case of a 16-year old girl with systemic lupus erythematosus who developed septic arthritis of the left knee. delayed diagnosis because of similar symptoms of arthritis due to lupus and purulent arthritis led to a destruction of the joint despite systemic antibiotic t ...200212235552
antidiarrhoeal properties of a novel sigma ligand (jo 2871) on toxigenic diarrhoea in mice: mechanisms of action.sigma ligands display antisecretory activity against various secretagogues, suggesting antidiarrhoeal properties. in this study, we evaluated: (i) the antidiarrhoeal effect of jo 2871, a high affinity sigma ligand, in three models of toxigenic diarrhoea in mice; and (ii) the site and mechanism of action of this compound.200212235074
salmonella spp. on chicken carcasses in processing plants in poland.chickens at selected points in the slaughter process and after slaughter on the dressing line in poultry plants were sampled and analyzed for salmonella. these chickens came from the northeast part of poland. the examinations were carried out in quarters i, ii, iii, and iv of 1999. all the birds were determined to be healthy by a veterinary inspection. swab samples were taken from the cloaca after stunning and from the skin surface and body cavity of the whole bird after evisceration, after rins ...200212233861
survival and growth of shigella flexneri, salmonella enterica serovar enteritidis, and vibrio cholerae o1 in reconstituted infant formula.formula feeding is an alternative method to prevent mother-to-child infection with human immunodeficiency virus through breast-feeding in developing countries. growth of bacterial pathogens in reconstituted infant formula has become a health hazard when contaminated water is used for rehydration. this study was conducted to assess bacterial safety risk of using contaminated water to reconstitute infant formula. survival and growth characteristics were determined for three bacterial pathogens, vi ...200212224592
endovascular treatment and complete regression of an infected abdominal aortic aneurysm.to report a case of successful endovascular treatment of an infected abdominal aortic aneurysm (aaa) following salmonella septicemia.200212223018
mitigation of avian reproductive tract function by salmonella enteritidis producing high-molecular-mass lipopolysaccharide.hens were infected with a wild-type salmonella enteritidis and its wzz mutant, which lacked the ability to make high-molecular-mass lipopolysaccharide (lps), in six experiments paired by dosage and route of exposure. involution of the reproductive tract occurred in 86% of hens that were injected subcutaneously with 108 cfu of the wild-type strain, but none did so when injected with the wzz mutant. in spite of the lack of a specific effect on the reproductive tract, infection of hens with the mut ...200212220411
diarrhea associated acute renal failure in a patient with salmonella enteritidis sepsis.salmonella enteritidis infection occurs primarily in animals and often results in self-limited gastroenteritis in accidental cross contamination in human. however, the acute renal failure could be a rare but serious complication of the s. enteritidis infection. we report one case of acute renal failure from severe dehydration caused by s. enteritidis food poisoning. the acute renal failure completely recovered after hydration and antibiotic treatment.200212212834
an outbreak of salmonella enteritidis phage type 34a infection in primary school children: the use of visual aids and food preferences to overcome recall bias in a case control study.outbreaks of infectious intestinal disease are common in schools. case control studies are useful in the investigation of infectious disease outbreaks but the time interval between illness and investigation can lead to recall bias, particularly in young children. we describe an outbreak of salmonella enteritidis phage type 34a infection involving 54 clinical cases in two adjacent schools, and a novel approach to overcome recall bias. the likely dates of infection were identified from the epidemi ...200212211594
salmonella surveillance: a global survey of public health serotyping.to better understand the global epidemiology of salmonellosis and the national surveillance programmes used for salmonella infections in humans, we conducted a global survey of the 191 who member states. we gathered information on the total number of salmonella isolates serotyped, and the 15 most commonly isolated serotypes from humans in 1990 and 1995. of the 104 countries that responded, 76 (73.1%) conducted public health surveillance for salmonella and 69 of these (90.8%) conducted serotyping ...200212211575
detection of experimental salmonella enteritidis and s. typhimurium infections in laying hens by fluorescence polarization assay for egg yolk antibodies.identifying infected laying flocks is a critical component in efforts to prevent eggborne transmission of salmonella enteritidis to humans. in the present study, egg yolk samples from experimentally infected chickens were tested for specific antibodies with a very rapid fluorescence polarization assay using tracers prepared from the o-polysaccharides of s. enteritidis and s. typhimurium and a conventional elisa using an s. enteritidis flagellin antigen. in two trials, groups of specific-pathogen ...200212211303
hla-b27 modulates nuclear factor kappab activation in human monocytic cells exposed to lipopolysaccharide.to study whether hla-b27 modifies some key factors controlling inflammatory responses on lipopolysaccharide (lps) stimulation in human monocytic cells.200212209522
emergence of klebsiella pneumoniae isolates producing inducible dha-1 beta-lactamase in a university hospital in taiwan.ten nonrepetitive clinical isolates of klebsiella pneumoniae exhibiting an unusual inducible beta-lactam resistance phenotype were identified between january 1999 and september 2001 in a university hospital in taiwan. in the presence of 2 micro g of clavulanic acid, the isolates showed a one to four twofold concentration increase in the mics of ceftazidime, cefotaxime, and aztreonam but remained susceptible to cefepime (mics, </=0.5 micro g/ml) and imipenem (mics, </=0.5 micro g/ml). pcr, sequen ...200212202541
different bacterial lipopolysaccharides as toxicants and stressors in the shrimp palaemon elegans.this study compares the in vivo haemocytic response of shrimp, palaemon elegans (rathke) to different types of lps injection. in particular it investigates to what degree and speed the haemocytopenia varies between lpss from different sources. it further compares the tolerated doses of different lpss in these animals and finds substantial differences in the various toxicity types. the work then relates this to blood glucose levels and stress-linked variations in glycaemic status. the order of lp ...200212201651
[pleural empyema due to salmonella enteritidis]. 200212199937
salmonella prosthetic joint septic arthritis.we describe a case of salmonella enteritidis infection of a prosthetic knee joint that was cured with ceftriaxone therapy for 6 weeks and replacement of the tibial component of the prosthesis. eleven other cases of salmonella prosthetic joint infection have been reported in the english-language literature. five infections occurred within 20 days of prosthesis placement, and seven occurred several months to years later; ten of 12 infections involved hip prostheses. nine of 12 patients who had pro ...200212199853
antibiotic resistance of moroccan strains of salmonella enteritidis isolated between 1996 and 1997.antimicrobial resistance is a worldwide problem. the antibiotic resistance of moroccan strains of salmonella enteritidis was investigated from 1996 to 1997. a total of 51 strains were collected within this period, 31 derived from human sources and 20 from food. of the 31 human strains, 10 were resistant to antibiotics; 4 were resistant to two or more antibiotics. of the 20 food strains, 11 were resistant to antibiotics; 6 were resistant to two or more antibiotics. the results are similar to thos ...200012197335
identification of genes affecting salmonella enterica serovar enteritidis infection of chicken macrophages.screening of 7,680 salmonella enterica serovar enteritidis mutants for attenuation in a chicken macrophage infection model yielded a series of mutants including several with defects in previously unrecognized salmonella virulence genes. one of the newly identified genes was the pbpa2 gene, belonging to the penicillin binding protein gene family.200212183592
potentiation of tumor metastasis in adulthood by neonatal endotoxin exposure: sex differences.previous research in rodents has demonstrated that neonatal exposure to bacterial endotoxin alters the hypothalamic-pituitary-adrenal (hpa) axis resulting in hypersecretion of corticosterone in response to stress in adulthood. given the known interactions between glucocorticoids and the immune system we tested the hypothesis that such alterations may impact on immune outcomes. male and female fischer 344 neonate rats were treated with endotoxin (0.05 mg/kg lipopolysaccaride from salmonella enter ...200212183215
inactivation of escherichia coli o157:h7, salmonella enterica serotype enteritidis, and listeria monocytogenes on lettuce by hydrogen peroxide and lactic acid and by hydrogen peroxide with mild heat.iceberg lettuce is a major component in vegetable salad and has been associated with many outbreaks of foodborne illnesses. in this study, several combinations of lactic acid and hydrogen peroxide were tested to obtain effective antibacterial activity without adverse effects on sensory characteristics. a five-strain mixture of escherichia coli o157:h7, salmonella enterica serotype enteritidis, and listeria monocytogenes was inoculated separately onto fresh-cut lettuce leaves, which were later tr ...200212182470
effects of asparaginase and polyphenol oxidase on adhesive characteristics of microorganisms.we studied the effects of polyphenol oxidase and asparaginase on microorganism adhesion to buccal epithelial cells. these enzymes reduced adhesion of pathogenic microorganisms (uropathogenic and escherichia coli, salmonella enteritidis, entamoeba spp., influenza virus, candida albicans, streptococcus spp.) and had virtually no effect on adhesive characteristics of probiotic variants of escherichia coli and lactobacillus fermentum.200212170308
screening of some siberian medicinal plants for antimicrobial activity.the antimicrobial activity of crude ethanolic extracts of 16 siberian medicinal plants was tested against five species of microorganisms: bacillus cereus, escherichia coli, staphylococcus aureus, pseudomonas aeruginosa, and candida albicans. of the 16 plants tested, 12 showed antimicrobial activity against one or more species of microorganisms. the most active antimicrobial plants were bergenia crassifolia, chelidonium majus, rhaponticum carthamoides, sanguisorba officinalis, and tussilago farfa ...200212169406
nitric oxide supports atrial function in sepsis: relevance to side effects of inhibitors in shock.the mechanisms underlying myocardial dysfunction in sepsis remain poorly understood. the theoretical benefits of nitric oxide synthase (nos) inhibition in reversing the haemodynamic changes that characterise septic shock have not been supported by clinical trials, some of which have demonstrated detrimental myocardial effects. we have therefore assessed the effects of endotoxaemia on nos enzyme expression as well as a number of functional responses of myocardial tissue from rats. atrial tissue e ...200212167470
prolonged fever with recurrent diarrhoea. 200212151666
surface plasmon resonance (biacore) detection of serum antibodies against salmonella enteritidis and salmonella typhimurium.we have used a surface plasmon resonance biosensor (biacore 3000) to detect serum antibodies in chickens having current or recent infections. three well-defined salmonella flagellar recombinant dna antigens reflecting salmonella enteritidis (h:g,m flagellin) and salmonella typhimurium (h:i and h:1,2 flagellins) expressed in escherichia coli were each immobilized in a single flow cell of a biosensor chip. glutathione-s-transferase was immobilized on the surface of another flow cell to monitor non ...200212133620
endotoxin potentiates the hepatotoxicity of cocaine in male mice.cocaine produces hepatotoxicity by a mechanism that remains undefined but that has been linked to its oxidative metabolism. endotoxin (lipopolysaccharide, lps) is also a well-known cause of hepatic damage, where exposure to non-injurious doses of lps increases the toxicity of certain hepatotoxins. this study was conducted to investigate the possible potentiation of cocaine-mediated hepatotoxicity (cmh) by lps. male cf-1 mice were administered oral cocaine hydrochloride for 5 consecutive days at ...200212133232
the effect of vaccination with a salmonella enteritidis aroa mutant on early cellular responses in caecal lamina propria of newly-hatched chickens.when newly hatched chicks are inoculated with a salmonella strain, they induce a rapid onset of resistance to intestinal colonization by other salmonella strains. the exact mechanism of this early colonization-inhibition is not known. to study host-related contributions to this phenomenon, the kinetics of immune cell infiltration in the caecal wall was analyzed during the first 10 days after vaccination of newly hatched chickens with a salmonella enterica serovar enteritidis aroa mutant, and inf ...200212126917
detection of a previously uncommon salmonella phage in tourists returning from europe. 200212126854
isolation of salmonella from muscoid flies at commercial animal establishments in san bernardino county, california.adult muscoid flies collected from three dairies and eight commercial poultry ranches in san bernardino county were tested for salmonella enteritidis. of the total 2,686 flies tested, 92.3% were musca domestica, 5.5% fannia canicularis, 1.9% ophyra leucostoma, 0.2% phaenicia sericata, and <0.1% muscina stabulans. whereas flies at the dairies belonged exclusively to m. domestica, faunal composition at the poultry ranches was more diverse, including all five species. among the five fly species, on ...200212125877
[purulent salmonella arthritis of the ankle joint].in the case study the authors focus on salmonella enteritidis which caused purulent osteoarthritis of the ankle and sepsis. salmonella osteoarthritis is one of the nidal forms of the salmonella infection. the authors briefly present etiology, pathogenesis, diagnosis and principles of the treatment of infectious osteoarthritis. they analyze a case of a 56 years old immunocompromised patient who was treated at the authors' department for a late diagnosis with an already developed septicemia. they ...200212125223
foodborne disease in our global village: a multinational investigation of an outbreak of salmonella serotype enteritidis phage type 4 infection in puerto vallarta, mexico.in late 1996, a multinational investigation was launched following an outbreak of diarrheal illness that caused the disruption of an international scientific conference at a first-class hotel in puerto vallarta, mexico.200212121595
a nucleic acid sequence-based amplification method to detect salmonella enterica serotype enteritidis strain pt4 in liquid whole egg.nucleic acid sequence-based amplification (nasba) was applied to the detection of salmonella enterica cells in liquid whole egg. samples (25 g) of liquid whole egg inoculated with salmonella cells were enriched for 16 h in buffered peptone water, and the nasba procedure was effective in detecting the presence of salmonella in samples inoculated with 10 to 100 cfu prior to enrichment.200212117254
differences between reference laboratories of the european community in their ability to detect salmonella species.the ability of national reference laboratories for salmonella of the european union member states to detect salmonella bacteria was tested in four collaborative studies during the period 1995 through 1999. three different methods were prescribed in the four studies. capsules containing various numbers of salmonella typhimurium or salmonella enteritidis were tested. in studies ii, iii and iv, salmonella bacteria were isolated in the presence of competitive microorganisms. significant differences ...200212111601
a rare case of salmonella soft-tissue abscess.a healthy 6-year-old boy had complained of fever and chest pain for 3 days. on admission, he had a mass on the sternum, 3.7 x 2.5 cm in size. abnormal laboratory findings included a white blood cell count of 12,900/microl, erythrocyte sedimentation rate (esr), 74 mm/h, and c-reactive protein (crp), 9.7 mg/dl. ultrasound examination of the chest revealed a hypoechoic lesion on the sternum that was 30 x 15 mm in size. chest computed tomography (ct) scan showed no bone fracture or bone erosion. the ...200212111575
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