Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| managing vaginal trichomoniasis resistant to high-dose metronidazole therapy. | 1997 | 9433954 | |
| incidence of trichomonas vaginalis among women having vaginal discharge, in manisa, turkey. | trichomoniasis characterise with a foamy yellowish odorous discharge, is an infection that causes superficial defects and necrotic ulcers in mucosa, and is spreadable with sexual contact. in t. vaginalis infection, the diagnosis can be made after the examination of saline wet mount preparations, stained smears preparation and cultivation of the vaginal discharge, the urine and the materials obtained from prostate secretion in males. in the present study, vaginal discharge samples obtained from 2 ... | 1997 | 9425833 |
| biochemical properties of a neuraminidase of trichomonas vaginalis. | trichomonas vaginalis possesses a membrane-associated neuraminidase activity that is released into culture medium during its growth in vitro. the neuraminidase shows an optimum ph of 4.5 and a km of 0.15 mm for 2'-(4-methylumbelliferyl)-alpha-d-n-acetyl-neuraminic acid as a substrate. this enzyme releases mainly alpha-2,3-linked sialic acid because it is able to liberate sialic acid from sialyllactose (mainly alpha-2,3) but not from mucin (alpha-2,6) or fixed erythrocytes (mainly alpha-2,6). the ... | 1997 | 9406769 |
| [vulvovaginitis]. | candidiasis, infection due to trichomonas vaginalis and bacterial vaginosis (gardnerella vaginalis and/or other species) represent the major three causes of vulvo-vaginitis. other are rare bacterial infections and non infectious vaginitis such as allergic and post-menopausal vaginitis with epithelial atrophy. clues for the diagnosis include the clinical features of vaginal discharge, cytological examinations, bacterial and fungal cultures. only t. vaginalis seems to be responsible of sexually tr ... | 1997 | 9406511 |
| comparison of the inpouch tv culture system and diamond's modified medium for detection of trichomonas vaginalis. | this study compared the use of diamond's modified medium to inpouch for the culture of trichomonas vaginalis from pooled vaginal secretions. the sensitivity for inpouch was 82.4% (61/74) versus 87.8% (65/74) for diamond's modified medium. there were no significant differences in the sensitivity and negative predictive value of inpouch compared to diamond's modified medium. | 1997 | 9399542 |
| molecular methods for diagnosis and epidemiological studies of parasitic infections. | direct microscopy is widely used for the diagnosis of parasitic infections although it often requires an experienced microscopist for accurate diagnosis, is labour intensive and not very sensitive. in order to overcome some of these shortcomings, molecular or nucleic acid-based diagnostic methods for parasitic infections have been developed over the past 12 years. the parasites which have been studied with these techniques include the human plasmodia, leishmania, the trypanosomes, toxoplasma gon ... | 1997 | 9394184 |
| contact-dependent disruption of the host cell membrane skeleton induced by trichomonas vaginalis. | this report presents evidence showing that the pathogenetic process of the protozoan parasite trichomonas vaginalis involves degradation of the target cell membrane skeleton; spectrin, the most representative protein within this structure, has been identified as the main molecular target. degradation of the target cell spectrin is accomplished only upon contact with the parasite, and immunochemical and immunofluorescence studies performed with the erythrocyte as a model demonstrate that degradat ... | 1997 | 9393808 |
| a comparison of the sensitivity of the inpouch tv, diamond's and trichosel media for detection of trichomonas vaginalis. | this study compared the ability of three culture media (inpouch tv, diamond's, and trichosel) to support the growth of clinical isolates of trichomonas vaginalis and their relative sensitivity for detection of the organism. | 1997 | 9389954 |
| the vagina has reducing environment sufficient for activation of trichomonas vaginalis cysteine proteinases. | trichomonas vaginalis, a worldwide distributed sexually transmitted protozoan, is remarkable for synthesis of numerous, distinct cysteine proteinases, the significance of which is evidenced by the presence in vivo of soluble proteinases in secretions and antiproteinase antibody in serum of patients with trichomonosis. these proteinases purportedly play a role in host parasitism and immune evasion. | 1997 | 9389953 |
| entamoeba histolytica: computer-assisted modeling of phosphofructokinase for the prediction of broad-spectrum antiparasitic agents. | pyrophosphate-dependent phosphofructokinase (ppi-pfk) is the rate-limiting glycolytic enzyme found in the pathogenic protists entamoeba histolytica, giardia lamblia, toxoplasma gondii, trichomonas vaginalis, and naegleria fowleri. the enzyme differs significantly from atp-dependent phosphofructokinases found in humans and as such represents an important drug target. current therapy for infections caused by these pathogens is inadequate, especially for children, pregnant women, and the immune com ... | 1997 | 9371084 |
| metronidazole. a therapeutic review and update. | the nitroimidazole antibiotic metronidazole has a limited spectrum of activity that encompasses various protozoans and most gram-negative and gram-positive anaerobic bacteria. metronidazole has activity against protozoans like entamoeba histolytica, giardia lamblia and trichomonas vaginalis, for which the drug was first approved as an effective treatment. anaerobic bacteria which are typically sensitive are primarily gram-negative anaerobes belonging to the bacteroides and fusobacterium spp. gra ... | 1997 | 9360057 |
| evolution of the hydrogenosome. | since its discovery almost 25 years ago the enigmatic hydrogenosome, a redox organelle of anaerobic unicellular eukaryotes, has puzzled evolutionists as to its origin and function. synthesis of recent molecular, physiological and morphological studies now favours the hypothesis that hydrogenosomes derived from a modification of pre-existing mitochondria, and argues against the previously held view that the hydrogenosome had a polyphyletic origin. these data provide evidence for a more ancient or ... | 1997 | 9351194 |
| trichomoniasis in a postmenopausal woman cured after discontinuation of estrogen replacement therapy. | epidemiologic and experimental data suggest that estrogen has a salutary effect on trichomonas vaginalis. | 1997 | 9339974 |
| the role of nitric oxide as an effector of macrophage-mediated cytotoxicity against trichomonas vaginalis. | the purpose of this study is to determine whether nitric oxide is involved in the extracellular killing of trichomonas vaginalis by mouse (balb/c) peritoneal macrophages and raw264.7 cells activated with lps or rifn-gamma and also to observe the effects of various chemicals which affect the production of reactive nitrogen intermediates (rni) in the cytotoxicity against t. vaginalis. the cytotoxicity was measured by counting the release of [3h]-thymidine from labelled protozoa and no2- was assaye ... | 1997 | 9335184 |
| hormonal contraception, vitamin a deficiency, and other risk factors for shedding of hiv-1 infected cells from the cervix and vagina. | factors that influence shedding of hiv-1 infected cells in cervical and vaginal secretions may be important determinants of sexual and vertical transmission of the virus. we investigated whether hormonal contraceptive use, vitamin a deficiency, and other variables were risk factors for cervical and vaginal shedding of hiv-infected cells. | 1997 | 9314871 |
| molecular characterization of a sarcoplasmic-endoplasmic reticulum ca+2 atpase gene from trichomonas vaginalis. | dna fragments homologous to p-type cation translocating atpase genes were identified in trichomonas vaginalis by polymerase chain reaction (pcr) amplification. the genomic locus corresponding to one pcr fragment, tvca1, contains a 3,055 base-pair open reading frame encoding a 108,162 dalton protein composed of 981 amino acids. tvca1 lacks introns, is present in a single copy, and is expressed as a 3.1 kb transcript with short 5' and 3' untranslated regions. separate primer extension experiments ... | 1997 | 9304817 |
| evaluation of a murine model of experimental trichomoniasis. | by using a reference strain of trichomonas vaginalis and the intraperitoneal route for infecting animals, the influence of the strain of mice, the time observation and the inoculation doses were followed in order to standardize the optimal conditions for the evolution of experimental trichomoniasis. our results suggest that the inoculation of balb/c mice with 10(7) trichomonads and the semiquantitative assessment at day 15 postinfection of the gross-pathologic changes in the abdominal cavity--pe ... | 1997 | 9296057 |
| colpitis macularis and macular vaginitis unrelated to trichomonas vaginalis infection. | 1997 | 9292350 | |
| use of antibiotics to prevent preterm birth. | our purpose is to review recent data and provide a clinical opinion on the use of antibiotics to prevent preterm birth or related maternal-neonatal complications. a literature review and a synthesis of opinion are provided. during prenatal care, standard practices should be applied regarding neisseria gonorrhoeae, chlamydia trachomatis, and bacteriuria. in addition, screen for and treat bacterial vaginosis in patients at high risk for preterm birth but do not treat ureaplasma urealyticum or grou ... | 1997 | 9290454 |
| [the microbial etiology of genital discharges in nouakchott, mauritania]. | the survey carried on 579 patients of both sexes, consulting for genital discharges (spontaneous or referred), showed that in 61.1% men cases, std agents were isolated (neisseria gonorrhoeae 51.5%) and 64.4% in women cases (gardnerella vaginalis 24%, candida albicans 20%. trichomonas vaginalis 14%). 17% of neisseria gonorrhoeae strains were penicillinase-producing (ppng). | 1997 | 9289258 |
| the relationship of bacterial vaginosis, candida and trichomonas infection to symptomatic vaginitis in postmenopausal women attending a vaginitis clinic. | to estimate the prevalence of bacterial vaginosis, candida albicans, and trichomonas vaginalis infections in a population of postmenopausal women with symptoms of vaginitis seen at a vaginitis clinic either as self-referred or clinician referred patients. | 1997 | 9288698 |
| trichomonas vaginalis: analysis of codon usage. | 1997 | 9287961 | |
| hiv-1 infection associated with abnormal vaginal flora morphology and bacterial vaginosis. | in-vitro research has suggested that bacterial vaginosis may increase the survival of hiv-1 in the genital tract. therefore, we investigated the association of hiv-1 infection with vaginal flora abnormalities, including bacterial vaginosis and depletion of lactobacilli, after adjustment for sexual activity and the presence of other sexually transmitted diseases (stds). | 1997 | 9284776 |
| tampon sampling for diagnosis of bacterial vaginosis: a potentially useful way to detect genital infections? | genital tract infections are important causes of ill health in developing countries, but diagnosis is difficult. bacterial vaginosis (bv) was correctly diagnosed by using a vaginal specimen obtained by tampon sampling in 22 of 24 women (91.6%) for whom bv was diagnosed by gram staining. the yield for other vaginal infections was higher (28% for trichomonas vaginalis and 32.7% for candida albicans) than it was for cervical infections (0% for neisseria gonorrhoeae and 30% for chlamydia trachomatis ... | 1997 | 9276426 |
| study on ultrastructural cytochemistry and pathogenic mechanism of trichomonas vaginalis. | to study the relation among enzymic ultrastructural localizations, cell organelles and functions of trichomonas vaginalis (t. vaginalis) as well as its pathogenic mechanism. | 1996 | 9275338 |
| isolation and characterization of cytosolic malate dehydrogenase from trichomonas vaginalis. | malate dehydrogenase (ec 1.1.1.37.) (mdh) was purified to apparent homogeneity from the cytosolic fraction of the protozoan trichomonas vaginalis donné. the four step purification included ion-exchange chromatography (deae-sephacel and q-sepharose, elution with nacl) and affinity chromatography (reactive red agarose, elution with nadh and nacl). the enzyme was purified about 132-fold (30.6% yield) to a specific activity of 352 units mg-1. the km values determined at ph 7.8 (ph optimum from 7.5 t ... | 1997 | 9269720 |
| a rapid diagnostic test for trichomonas vaginalis infection. | the applicability of the quantitative buffy coat tube test (qbc) for diagnosis of t. vaginalis was studied. the results of the qbc tube test was compared with that of the wet mount examination in 60 women having genital infection. the cases were selected from outpatient clinic of el-shatby university hospital t. vaginalis was identified by qbc tube test in 24 cases out of 60 while the wet mount smear, it was detected in 21 cases. the sensitivity of the qbc tube test was 100% and the specificity ... | 1997 | 9257972 |
| molecular cloning of actin genes in trichomonas vaginalis and phylogeny inferred from actin sequences. | the parasitic protozoan trichomonas vaginalis is known to contain the ubiquitous and highly conserved protein actin. a genomic library and a cdna library have been screened to identify and clone the actin gene(s) of t. vaginalis. the nucleotide sequence of one gene and its flanking regions have been determined. the open reading frame encodes a protein of 376 amino acids. the sequence is not interrupted by any introns and the promoter could be represented by a 10 bp motif close to a consensus mot ... | 1997 | 9252588 |
| extracellular release by trichomonas vaginalis of a nadp+ dependent malic enzyme involved in pathogenicity. | this report presents evidence showing that trichomonas vaginalis releases in the extracellular environment a functional form of nadp(+)-dependent malic enzyme. the protein which is likely responsible for the oxidative decarboxilase activity had already been identified in previous studies as p65, one of the five adhesive proteins of the protozoan. the same protein had also been described as ap65 by other authors, which identified it as one of the four surface proteins specifically responsible for ... | 1997 | 9250781 |
| evidence for loss of mitochondria in microsporidia from a mitochondrial-type hsp70 in nosema locustae. | in molecular phylogenies based on ribosomal rna, three amitochondriate protist lineages, microsporidia, metamonada (including diplomonads) and parabasala (including trichomonads), are the earliest offshoots of the eukaryotic tree. as an explantation for the lack of mitochondria in these organisms, the hypothesis that they have diverged before the mitochondrial endosymbiosis is preferred to the less parsimonious hypothesis of several independent losses of the organelle. nevertheless, if they had ... | 1997 | 9247927 |
| trichomonas vaginalis associated with low birth weight and preterm delivery. the vaginal infections and prematurity study group. | several studies have suggested that pregnant women infected with trichomonas vaginalis may be at increased risk of an adverse outcome. | 1997 | 9243743 |
| conservation of mitochondrial targeting sequence function in mitochondrial and hydrogenosomal proteins from the early-branching eukaryotes crithidia, trypanosoma and trichomonas. | kinetoplastid protozoa are the earliest-branching eukaryotes to possess a true mitochondrion. this organelle is host to a variety of intriguing and unique features, including rna editing. we examined the characteristics of protein import into mitochondria of trypanosoma brucei. dihydrofolate reductase (dhfr) carrying a yeast mitochondrial targeting signal was correctly translocated into trypanosome mitochondria in vivo, as were dhfr fusion proteins bearing two unusually short (7-9 amino acids) p ... | 1997 | 9243185 |
| synthesis and antiparasitic activities of amidinic azolated derivatives. | a set of heterocyclic n-acetamidinium hydrochlorides were prepared from the corresponding n-acetonitriles. the antiparasitic screening showed that, while all amidines are practically inactive, some nitriles present leishmanicide properties. | 1997 | 9241827 |
| molecular approaches to the diagnosis of male infertility. | the introduction of the polymerase chain reaction (pcr) as a technique to selectively amplify and identify specific dna and rna sequences has revolutionized the field of molecular medicine. application of these newly developed molecular techniques to the field of male infertility has made the delineation of subtle causes of infertility-an inapparent genital tract infection, immune system activation within the genital tract, mutations in sperm mitochondrial or chromosomal dna, alterations in sper ... | 1996 | 9238679 |
| arsenical pessaries in the treatment of metronidazole-resistant trichomonas vaginalis. | 1997 | 9228600 | |
| a self-administered technique for the detection of sexually transmitted diseases in remote communities. | the control of sexually transmitted diseases (stds) in remote rural communities would be enhanced by a sensitive self-administered method for the detection of asymptomatic infection. results of conventional methods for the detection of stds were compared with results of tampon-collected specimens analyzed by polymerase chain reaction (pcr) for 480 women. neisseria gonorrhoeae, chlamydia trachomatis, and trichomonas vaginalis were detected by routine methods in 4 (1%), 14 (3%), and 41 (9%) sample ... | 1997 | 9207385 |
| chromosome number of trichomonas vaginalis. | chromosome number of an axenically grown trichomonas vaginalis isolate was studied using a 1 mm solution of colchicine and a hypotonic swelling technique. the diploid chromosome number was 2n = 6 [corrected]. each pair of chromosomes can be identified by its morphology and size. this observation could be important with respect to gene mapping and molecular cloning for genes of t. vaginalis. | 1997 | 9194850 |
| involvement of dsrna virus in the protein composition and growth kinetics of host trichomonas vaginalis. | trichomonas vaginalis harbors a double-stranded (ds)-rna virus, and the presence of virus is related to upregulated expression and phenotypic variation of a prominent immunogen (khoshnan a, alderete jf (1994) j virol 68: 4035-4038). to further test the influence of virus on t. vaginalis, virus-infected (v+) isolates were compared to virus-free (v-), agar-cloned progeny trichomonads derived from the parental isolates for accumulation of total proteins and cysteine proteinases. comparative high re ... | 1997 | 9191859 |
| pyridazine n-oxides. ii. synthesis and in vitro antimicrobial evaluation of 3-chloro-4-carbamoyl-5-aryl-6-methyl-pyridazine n-oxides. | as a part of a research project on antimicrobial agents, various novel carbamoyl derivatives of pyridazine-n-oxides 7a-j were prepared in moderate to good yields from 3-chloro-4-ethoxycarbonyl-5-aryl-6-methyl-3-pyridazine. all compounds synthesized were ineffective against gram+, gram- bacteria and fungi while 7e and 7j exhibited a fairly good activity against trichomonas vaginalis. | 1997 | 9181685 |
| clinical and epidemiological correlations between the infection with hpv 16 and hpv 18 and female cervical lesions. | a number of 66 specimens from female cervical lesions were examined for infection with human papillomavirus (hpv) types 6, 11, 16, and 18 by nucleic acid hybridization in dot-blot techniques and 35 sera were tested by the immunodot-blot technique, in order to detect the presence of anti e4 and e7 hpv protein antibodies. the findings were compared with the histologic diagnosis. fifty-six per cent of specimens contained hpv dna sequences. in 47% of specimens from cervical carcinoma, hpv 11 was det ... | 1995 | 9179967 |
| actin cytoskeleton demonstration in trichomonas vaginalis and in other trichomonads. | the flagellate form of trichomonas vaginalis (t v) transforms to amoeboid cells upon adherence to converslips. they grow and their nuclei divide without undergoing cytokinesis, yielding giant cells and a monolayer of t v f-actin was demonstrated in trichomonas vaginalis by fluorescence microscopy using phalloidin and an anti-actin mab which labelled the cytoplasm of both the flagellate and amoeboid forms. comparative electrophoresis and immunoblotting established that the actin band has the same ... | 1996 | 9175265 |
| evidence for the bacterial origin of genes encoding fermentation enzymes of the amitochondriate protozoan parasite entamoeba histolytica. | entamoeba histolytica is an amitochondriate protozoan parasite with numerous bacterium-like fermentation enzymes including the pyruvate:ferredoxin oxidoreductase (por), ferredoxin (fd), and alcohol dehydrogenase e (adhe). the goal of this study was to determine whether the genes encoding these cytosolic e. histolytica fermentation enzymes might derive from a bacterium by horizontal transfer, as has previously been suggested for e. histolytica genes encoding heat shock protein 60, nicotinamide nu ... | 1997 | 9171424 |
| the minimum single oral metronidazole dose for treating trichomoniasis: a randomized, blinded study. | to identify the minimum effective single oral dose of metronidazole for trichomoniasis. | 1997 | 9166304 |
| std rapid assessment in rwandan refugee camps in tanzania. | to obtain baseline information on sexually transmitted diseases (stds) in the rwandan refugees camps in tanzania, prior to establishment of std services. | 1997 | 9155553 |
| validity of self-reported sexual behaviors in adolescent women using biomarker outcomes. | to determine the criterion-related validity of alternative approaches to the measurement of sexual intercourse using sexually transmitted diseases (std) as a biomarker (the criterion). | 1997 | 9153734 |
| sexual behavior risk factors associated with bacterial vaginosis and chlamydia trachomatis infection. | few studies have demonstrated that bacterial vaginosis (bv) is associated with sexual behavior risk factors similar to those for other sexually transmitted diseases. the objective of this study was to determine the prevalence of these factors in a multivariate analysis of data from women infected with bv and chlamydia trachomatis, and noninfected control subjects. | 1997 | 9153730 |
| polymerase chain reaction analysis of distal vaginal specimens: a less invasive strategy for detection of trichomonas vaginalis. | we compared polymerase chain reaction (pcr) analysis of specimens obtained from the distal vagina with wet mount microscopy and culture of specimens from the posterior vaginal fornix. one or all three techniques revealed that 61 (20.3%) of 300 women tested were positive for trichomonas vaginalis. pcr analysis of distal vaginal specimens detected 56 (91.8%) of 61 infections, while wet mount microscopy and culture detected 49 (80.3%) of 61 infections. results of this study may impact the approach ... | 1997 | 9142807 |
| multicenter comparison of clotrimazole vaginal tablets, oral metronidazole, and vaginal suppositories containing sulfanilamide, aminacrine hydrochloride, and allantoin in the treatment of symptomatic trichomoniasis. | trichomonas vaginalis is a common vaginal pathogen. oral metronidazole is the drug of choice for the treatment of trichomoniasis. oral metronidazole, however, may cause unpleasant side effects and is contraindicated during the first trimester of pregnancy. in vitro studies and preliminary clinical data have suggested that intravaginal clotrimazole may be effective against this pathogen. | 1997 | 9132982 |
| sexual transmission of hepatitis b in mwanza, tanzania. | hepatitis b virus (hbv) is endemic and poses a grave public health problem in africa where it is mainly transmitted from mother to baby or during childhood. sexual transmission has also been suggested to play a role in east africa, but this has never been properly demonstrated. additional preventive strategies may be proposed if sexual transmission of hbv occurred in this region where hiv and other stds are highly prevalent. | 1997 | 9132977 |
| organelle origins: energy-producing symbionts in early eukaryotes?. | the discovery that trichomonas vaginalis, an early diverging protist that lacks mitochondria but has energy-producing hydrogenosomes, makes bacterial-like heat shock proteins suggests that symbionts ancestral to mitochondria and hydrogenosomes were present at early stages of eukaryote evolution. | 1997 | 9115381 |
| transient and selectable transformation of the parasitic protist trichomonas vaginalis. | we have developed methods to transiently and selectably transform the human-infective protist trichomonas vaginalis. this parasite, a common cause of vaginitis worldwide, is one of the earlier branching eukaryotes studied to date. we have introduced three heterologous genes into t. vaginalis by electroporation and have used the 5' and 3' untranslated regions of the endogenous gene alpha-succinyl coa synthetase b (alpha-scsb) to drive transcription of these genes. transient expression of two repo ... | 1997 | 9114057 |
| evaluation of a screening algorithm for the diagnosis of genital infections with neisseria gonorrhoeae and chlamydia trachomatis among female sexworkers in bénin. | in developing countries, simple and cheap procedures for the diagnosis of sexually transmitted diseases (stds) are urgently needed, especially for screening purposes in high risk groups. | 1997 | 9111757 |
| immunological detection and subcellular localization of hsp70 and hsp60 homologs in trichomonas vaginalis. | heat shock protein (hsp) homologs of trichomonas vaginalis belonging to hsp70 and hsp60 families with mr 72 and 58 kda, respectively, were detected by cross-reacting polyclonal antibody to dnak of escherichia coli. polyclonal antibody to hsp60 of heliothis virescens, and polyclonal antibody to groel/hsp60 of cyanobacteria. a diffuse and finely granular intracellular staining of t. vaginalis was obtained with anti-dnak on frozen 4-micron sections of the parasite as revealed by light microscopy. t ... | 1997 | 9105301 |
| use of inhibitors to identify essential cysteine proteinases of trichomonas vaginalis. | designing cysteine proteinase inhibitors as antitrichomonal drugs requires knowledge of which cysteine proteinases are essential to the parasite. in an attempt to obtain such information, the effects of a number of cysteine proteinase inhibitors on trichomonad growth in vitro and proteinase activity were investigated. the broad specificity inhibitor trans-epoxysuccinyl-l-leucylamido-(4-guanidino)butane (known as e-64) had little effect on growth of trichomonas vaginalis (27% inhibition at 280 mi ... | 1997 | 9103977 |
| degradation of human immunoglobulins and cytotoxicity on hela cells by live trichomonas vaginalis. | the present study was undertaken to determine whether live t. vaginalis degrades human secretory iga, serum iga and igg molecules. human immunoglobulins were exposed to live trophozoites, parasite lysate, and excretory-secretory product (esp) of t. vaginalis. to determine the fragmentation of immunoglobulins, the reaction sample was subjected to sds-page and eitb, and peroxidase conjugated antihuman iga and igg were used as probes. live trophozoites degraded secretory iga. serum iga and igg, and ... | 1997 | 9100438 |
| one-tube, nested-pcr assay for the detection of trichomonas vaginalis in vaginal discharges. | to shorten the time and improve the accuracy of diagnosis of vaginal trichomoniasis, a novel, one-tube, nested pcr, which targets a family of 650-bp specific dna repeats from the trichomonas vaginalis genome, has been developed. samples were prepared by a rapid boiling method and the pcr products analysed by gel electrophoresis. a 290-bp dna fragment was observed in all positive cases. no cross-reaction with any other pathogens, including the pentatrichomonas hominis and giardia lamblia used as ... | 1997 | 9093430 |
| urethral infection in a workplace population of east african men: evaluation of strategies for screening and management. | transport workers (n = 504) in mombasa, kenya, were screened for urethral infection by history, clinical examination, and laboratory testing of urethral swabs and first-catch urine specimens. the prevalence of neisseria gonorrhoeae was 3.4%, chlamydia trachomatis, 3.6%, and trichomonas vaginalis, 6.0%; more than two-thirds of infections were asymptomatic. a complaint of urethral discharge, dysuria, or both was twice as sensitive as the sign of discharge on physical examination (34.5% vs. 15.5%) ... | 1997 | 9086138 |
| semisynthetic derivatives of purpuromycin as potential topical agents for vaginal infections. | purpuromycin (1) is an antibiotic with a broad spectrum of antimicrobial activity, encompassing bacteria, fungi, and protozoa, particularly those involved in vaginal infections. with the aim of enhancing the solubility and reducing the serum binding, a chemical program of modifications was undertaken on the natural compound, and a new interesting series of derivatives at the naphthoquinone system was synthesized and evaluated as potential topical agents for vaginal infections. in particular thre ... | 1997 | 9083486 |
| specific and sensitive detection of trichomonas tenax by the polymerase chain reaction. | a polymerase chain reaction (pcr) protocol was developed for specific detection of trichomonas tenax by using a pair of primers designed for its 18s rrna gene. the detection was specific for t. tenax, since no amplification was detected with dnas from trichomonas vaginalis, which belongs to the same genus as t. tenax, in addition to various species of oral protists, fungi and bacteria, and human leukocytes. this method had a detection limit of 100 fg for t. tenax genomic dna and could detect t. ... | 1997 | 9080698 |
| morphogenesis of the hydrogenosome: an ultrastructural study. | the morphogenesis of hydrogenosomes in several trichomonad species (tritrichomonas foetus, trichomonas vaginalis, tritrichomonas suis, trichomonas gallinae, tritrichomonas augusta and monocercomonas sp) was investigated by transmission electron microscopy of thin sections and freeze-fracture replicas of whole cells or the isolated organelle. close proximity, and even continuity, between endoplasmic reticulum and hydrogenosomes was observed. structures were seen connecting hydrogenosomes to each ... | 1996 | 9075329 |
| rapid antigen tests for neisseria gonorrhoeae and chlamydia trachomatis are not accurate for screening women with disturbed vaginal lactobacillary flora. | we studied the accuracy of the rapid antigen detection tests gonozyme and chlamydiazyme in high-risk women in an outpatient prenatal clinic, kalafong university hospital, pretoria, south africa. women (n = 433) presenting with uneventful pregnancy (n = 324), unavoidable miscarriage (n = 41) or infertility of 1 year's duration (n = 68) had a pap smear for lactobacillary grading and detection of pathogens like candida albicans or trichomonas vaginalis, a swab for culture of neisseria gonorrhoeae, ... | 1996 | 9060056 |
| hiv and reproductive tract infections in a total village population in rural kilimanjaro, tanzania: women at increased risk. | the aim of this study was to determine the prevalence of hiv infection, other sexually transmitted diseases (stds), and biological risk factors associated with hiv infection in a rural population in tanzania. a population-based study of a village population was carried out from july 1991 through january 1992. a total of 3,239 people (83.7%) participated in an hiv serosurvey. the total hiv prevalence was 0.7 and 1.9% among males and females, respectively (odds ratio, or = 2.5; 95% confidence inte ... | 1997 | 9052726 |
| sexually transmitted diseases among women in coventry. | 1997 | 9050291 | |
| organelle genomes: going, going, gone! | 1997 | 9036544 | |
| pyruvate: ferredoxin oxidoreductase from entamoeba histolytica recognized by a monoclonal antibody. | a mouse monoclonal antibody, eh208c2-2 mab, raised against whole cell antigens of entamoeba histolytica trophozoites of the pathogenic strain hm-1: imss and polyclonal antisera (pab) against membrane antigens of e. histolytica trophozoites of strain hth-56: mutm were screened against a cdna library of the pathogenic strain, sfl3. the monoconal antibody detected many phage plaques expressing an e. histolytica protein. the dna sequence encoding the protein was approximately 55% identical, over 1,1 ... | 1996 | 9031403 |
| iron-ascorbate cleavable malic enzyme from hydrogenosomes of trichomonas vaginalis: purification and characterization. | two isoforms of nad(p)(+)-dependent malic enzyme (ec 1.1.1.39) were isolated from hydrogenosomes of trichomonas vaginalis. a positively charged isoform at ph 7 was obtained in a single purification step using cation-exchange chromatography. the second isoform, negatively charged at ph 7.5, was partially purified using a combination of anion-exchange and affinity chromatography. both isoforms displayed similar physical and kinetic properties. molecular weight determination of the native enzyme su ... | 1996 | 9027755 |
| [microbiological study of some microorganisms implicated in sexually transmitted diseases among the female prison population]. | background: sexually transmitted diseases (std) are of over greater interest, particularly since the appearance of the human immunodeficiency virus ( hiv) in the last decade. asymptomatic or oligosymptomatic infections are very frequent, overall in women. the aim of this study was to know the prevalence of certain std producing microorganisms in a female penitentiary population, the clinical characteristics and evaluate the risk factors which may be found in this population. methods: one hundred ... | 1996 | 9011204 |
| [synthesis and antiparasitic activity of new n-alkyl derivatives of 1-(nitrophenyl)-3-(methyl-3-indolyl)-prop-2-ene-1-ones (nitroindolylchalcones)]. | 1996 | 9008902 | |
| metabolic adaptation of trichomonas vaginalis to growth rate and glucose availability. | the parasitic protist trichomonas vaginalis adapted the specific activities of twelve of the enzymes involved in glucose metabolism to the growth rate an glucose availability. these changes in enzyme activities were induced by culturing t. vaginalis in chemostats with glucose, present in rate-limiting or excess concentrations, as carbon and energy source. the specific activities were measured in pelleted cells at each steady state, while metabolic end products were determined in filtered culture ... | 1996 | 9004498 |
| scsb, a cdna encoding the hydrogenosomal beta subunit of succinyl-coa synthetase from the anaerobic fungus neocallimastix frontalis. | a clone containing a neocallimastix frontalis cdna assumed to encode the beta subunit of succinyl-coa synthetase (scsb) was identified by sequence homology with prokaryotic and eukaryotic counter-parts. an open reading frame of 1311 bp was found. the deduced 437 amino acid sequence showed a high degree of identity to the beta-succinyl-coa synthetase of escherichia coli (46%), the mitochondrial beta-succinyl-coa synthetase from pig (48%) and the hydrogenosomal beta-succinyl-coa synthetase from tr ... | 1996 | 9003318 |
| sustained estrogenization is insufficient to support long-term experimentally induced genital trichomonas vaginalis infection in balb/c mice. | 1996 | 9001187 | |
| high frequency of sexually transmitted diseases among pregnant women in dar es salaam, tanzania: need for intervention. | in order to determine the prevalence and characteristics of sexually transmitted diseases (stds) in pregnant women (pw) attending a primary health care antenatal clinic (anc) in metropolitan dar es salaam, tanzania, a randomly selected sample of pw in their second or third trimesters were invited to participate at their first visit. they were interviewed using a questionnaire and underwent genital examination. genital swabs were obtained for microscopy and/or culture isolation of candida albican ... | 1996 | 8997849 |
| prevalence of trichomonas vaginalis in prostitutes in turkey. | prostitutes are an important group for the transmission of a number of sexually transmitted diseases (std) all over the world. infection with t. vaginalis is one of the most common. in this study, the incidence and diagnostic procedures for trichomonas vaginalis infection as one of the sexually transmitted diseases (std's) have been studied. in legal prostitutes in ankara, turkey, 64 (25%) of 225 vaginal wet smears were positive for t. vaginalis. there was no significant difference between the d ... | 1996 | 8996719 |
| the sequence and organization of the core histone h3 and h4 genes in the early branching amitochondriate protist trichomonas vaginalis. | among the unicellular protists, several of which are parasitic, some of the most divergent eukaryotic species are found. the evolutionary distances between protists are so large that even slowly evolving proteins like histones are strongly divergent. in this study we isolated cdna and genomic histone h3 and h4 clones from trichomonas vaginalis. two histone h3 and three histone h4 genes were detected on three genomic clones with one complete h3 and two complete h4 sequences. h3 and h4 genes were ... | 1996 | 8995053 |
| comparable growth of a trichomonas vaginalis strain in pehps and tyi-s-33 media. | pehps medium, developed for axenic cultivation of entamoeba histolytica and e. invadens, was also capable of supporting the growth of a trichomonas vaginalis strain, with an inoculum of 1 to 100 trichomonads/ml. the logarithmic growth phase in pehps or in tyi-s-33 medium lasted 72 h; yield (3.33 +/- 0.56 x 10(6) trichomonads/ml), duplication time (4.27 h), number of duplications (16.85), or increase ratio (33,328) in pehps medium showed no significant differences with those obtained in tyi-s-33 ... | 1996 | 8987196 |
| processing trichomonas vaginalis for scanning electron microscopy. | 1996 | 8987030 | |
| detection of chlamydia trachomatis by immunofluorescence, papanicolaou and immunoperoxidase in women with leucorrhea. | bacteriological, papanicolaou, direct immunofluorescense (dif) and immunoperoxidase studies were done in 245 samples from women with vaginal discharge and the frequency of chlamydia trachomatis was investigated. the samples were obtained from two hospitals of the public health services of mexico city. samples were taken from the endocervical and posterior fornix areas and streaked in different cultures media for the isolation and microbiological differentiation. smears were done and stained by g ... | 1996 | 8986105 |
| [microbial associations in trichomonas urethritis]. | the results on isolation of associated microflora from 190 patients suffering from trichomonas urethritis are given. microorganisms belonged to the species: mycoplasma hominis. ureaplasma urealyticum. streptococcus pyogenes. escherichia coli. klebsiella pneumoniae. proteus mirabilis. moraxella lacunata. acinetobacter calcoaceticus and candida. staphylococcus cultures which were isolated from 100% patients belonged to 8 species: staphylococcus aureus, s. intermedius, s. haemolyticus, s. epidermid ... | 1996 | 8983524 |
| [management of male urethritis]. | 1996 | 8978204 | |
| linked genes for calmodulin and e2 ubiquitin-conjugating enzyme in trichomonas vaginalis. | in searching the genomes of early-diverging protists to study whether the possession of calmodulin is ancestral to all eukaryotes, the gene for calmodulin was identified in trichomonas vaginalis. this flagellate is a member of the parabasalia, one of the earliest lineages of recognized eukaryotes to have diverged. this sequence was used to isolate a homologous 1.250-kb fragment from the t. vaginalis genome by inverse polymerase chain reaction. this fragment was also completely sequenced and show ... | 1996 | 8976604 |
| colorimetric one-tube nested pcr for detection of trichomonas vaginalis in vaginal discharge. | a colorimetric one-tube nested pcr was developed for the detection of trichomonas vaginalis in clinical vaginal discharge specimens. a family of 650-bp specific dna repeats from the t. vaginalis genome was targeted. there was no cross-reaction with human dna or other infectious agents, including pentatrichomonas hominis and giardia lamblia. the colorimetric assay was applied as an adjunct to nested pcr for semiquantitative determination of t. vaginalis dna at levels corresponding to 1 to 1,000 p ... | 1997 | 8968894 |
| [clinico-microbiological findings in women with a genital discharge]. | the authors compare the prevalence of the most common vaginal infections at present and before 10 years and find that the prevalence of trichomonas vaginalis infection is diminished and there is a tendency for the increase in the incidence of bacterial vaginosis. the determination of the vaginal ph and the application of 10% koh for the "sniff test" may prove very useful in the diagnosis of vaginal infection. | 1996 | 8967532 |
| a possible mitochondrial gene in the early-branching amitochondriate protist trichomonas vaginalis. | trichomonads are anaerobic flagellated protists that, based on analyses of ribosomal rna sequences, represent one of the earliest branching lineages among the eukaryotes. the absence of mitochondria in these organisms coupled with their deep phylogenetic position has prompted several authors to suggest that trichomonads, along with other deeply-branching amitochondriate protist groups, diverged from the main eukaryotic lineage prior to the endosymbiotic origin of mitochondria. in this report we ... | 1996 | 8962102 |
| presence of a mitochondrial-type 70-kda heat shock protein in trichomonas vaginalis suggests a very early mitochondrial endosymbiosis in eukaryotes. | molecular phylogenetic analyses, based mainly on ribosomal rna, show that three amitochondriate protist lineages, diplomonads, microsporidia, and trichomonads, emerge consistently at the base of the eukaryotic tree before groups having mitochondria. this suggests that these groups could have diverged before the mitochondrial endosymbiosis. nevertheless, since all these organisms live in anaerobic environments, the absence of mitochondria might be due to secondary loss, as demonstrated for the la ... | 1996 | 8962101 |
| [prevalence of bacterial vaginitis and vaginosis: association with clinical and laboratory features, and treatment]. | this was a cross-sectional study meant to determine the prevalence of vaginitis and bacterial vaginosis among open population females from cuernavaca city. the relationship between clinical manifestations, laboratory diagnosis and response to therapy were evaluated as well. a group of 405 sexually active women were enrolled between january and july, 1994. the patients were attending the city hospital for a regular gynecological consultation, upon their informed consent, they answered a specifica ... | 1996 | 8948921 |
| sequence of a malic enzyme gene of giardia lamblia. | the nucleotide sequence and predicted amino acid sequence of malate dehydrogenase (decarboxylating) or malic enzyme (ec 1.1.1.40) of the amitochondriate protist giardia lamblia were determined. the overall amino acid identity with malic enzyme sequences from other eukaryotes was between 34 and 39%. functional domains previously defined in other malic enzymes, the malate-, the adp- and the nad(p)-binding domains, were present also in the g. lamblia sequence. in phylogenetic reconstructions, the g ... | 1996 | 8946381 |
| prokaryotic dna sequences in patients with chronic idiopathic prostatitis. | half of all men experience symptoms of prostatitis at some time in their lives, but the etiology is unknown for more than 90% of patients. optimal clinical and culture methods were used to select 135 men with chronic prostatitis refractory to multiple previous courses of antimicrobial therapy. the subjects had no evidence of structural or functional lower genitourinary tract abnormalities of bacteriuria or bacterial prostatitis by traditional clinical tests, or of urethritis or urethral pathogen ... | 1996 | 8940458 |
| [preliminary study on low-trait genital infection and cervical epithelial dysplasia in women from the parakanä tribe of south america]. | we evaluated the occurrence of low-trait genital infection and cervical epithelial dysplasia in women from a south-american indian tribe in the brazilian amazonia. | 1996 | 8935669 |
| clinical and microbiological correlates of vaginal trichomoniasis during pregnancy. the vaginal infections and prematurity study group. | colonization with trichomonas vaginalis is a possible cause of poor pregnancy outcome. to facilitate the diagnosis of this condition during pregnancy, we conducted a prospective, multicenter study of 13,816 gravid women who were between 23rd and 26th week of gestation. findings significantly associated with t. vaginalis colonization included a yellow, green, or bloody discharge from the vagina or cervix; abnormal odor after koh was added to a vaginal specimen; a vaginal ph of > 5.0; and cervical ... | 1996 | 8922806 |
| identification and characterization of [fe]-hydrogenases in the hydrogenosome of trichomonas vaginalis. | 1996 | 8920017 | |
| molecular characterization and expression of a n-acetylneuraminate lyase gene from trichomonas vaginalis. | 1996 | 8920014 | |
| etiologic study of genitourinary infections in women of childbearing age in bobo-dioulasso, burkina faso, 1992. | genitourinary infections have a major impact on public health, especially in africa. relative distribution of the different pathogens is unknown in bobo-dioulasso. | 1996 | 8919743 |
| trichomonas vaginalis adhesin proteins display molecular mimicry to metabolic enzymes. | 1996 | 8895795 | |
| molecular characterisation of adenosylhomocysteinase from trichomonas vaginalis. | the enzyme s-adenosylhomocysteine hydrolase (sahh) has been identified as a potential target for chemotherapy in protozoan parasites including trichomonas vaginalis. to investigate this area of trichomonad metabolism in more detail, we have isolated and characterised a gene which encodes this activity from the waa38 strain of this parasite. the gene was isolated by probing a bg/ii genomic mini-library with a fragment of the gene generated by thermal cycling using degenerate oligonucleotide prime ... | 1996 | 8892301 |
| phylogenetic implication of iron-containing superoxide dismutase genes from trichomonad species. | 1996 | 8892298 | |
| bacterial vaginosis: prevalence in an italian population of asymptomatic pregnant women and diagnostic aspects. | the present study has been carried out with the main objective of determining the prevalence rate of bacterial vaginosis (bv) in a population of asymptomatic pregnant women (8th to 9th month of pregnancy). out of a total 1,441 patients examined bv was found to be present in 70 (4.9%). the diagnosis was made when an analysis of the vaginal discharge revealed the simultaneous presence of at least three out of four of the following laboratory indices: (1) direct gram stain positive (bacterial flora ... | 1996 | 8891543 |
| epidemiology of trichomonas vaginalis. a prospective study in china. | trichomonas vaginalis infection is probably one of the most common sexually transmitted diseases. the epidemiology of this disease has long been ignored, and not much is known about its natural history and risk factors. | 1996 | 8885075 |
| use of wet mount to predict chlamydia trachomatis and neisseria gonorrhea cervicitis in primary care. | cervicitis is associated with salpingitis, infertility, and complications of pregnancy. universal screening has been recommended for high-prevalence populations but may not be appropriate in the family practice setting. leukocytes on an endocervical gram stain have been associated with infectious cervicitis due to chlamydia trachomatis and neisseria gonorrhea. this study sought to determine whether the finding of leukocytes in a vaginal wet mount could be used to screen for infectious cervicitis ... | 1996 | 8884256 |
| electrostatic effects in electron transfer reactions of [2fe-2s] ferredoxins with inorganic reagents. | the kinetics of electron transfer from the reduced [2fe-2s] ferredoxins from the cyanobacterium anabaena 7120 and the protozoan trichomonas vaginalis to select cobalt coordination compounds have been studied in order to gain insight into the mechanism of electron transfer and intrinsic reactivity of [2fe-2s] active sites. with tripositive cobalt complexes, reactions of both proteins displayed saturation kinetics; values of association constants of 12,900 and 1,400 m-1 and limiting rate constants ... | 1996 | 8880903 |
| modified thioglycolate medium: a simple and reliable means for detection of trichomonas vaginalis. | despite the declining rate of sexually transmitted diseases in developed countries, trichomoniasis is still one of the most common venereal infections. while diagnosis of this condition is commonly based on the microscopic wet-mount method, culture remains the most accurate single procedure for detecting the presence of trichomonas vaginalis in clinical samples. in the present study, the efficacy of a modified formula of the commonly available thioglycolate medium was compared with that of the s ... | 1996 | 8880540 |