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effects of medium-chain fatty acids on the structure and immune response of ipec-j2 cells.medium-chain fatty acids (mcfas) have been suggested as an alternative to the use of antibiotics in animal nutrition with promising results. first, we studied the sensitivity of salmonella enteritidis and an enteropathogenic escherichia coli strain against caprylic (c8), capric (c10) and lauric (c12) acids. a porcine in vitro model using the porcine cell line ipec-j2 was used to test the effects of mcfas on structural and immunological traits without and with a concomitant challenge with e. coli ...201627553650
distinct salmonella enteritidis lineages associated with enterocolitis in high-income settings and invasive disease in low-income settings.an epidemiological paradox surrounds salmonella enterica serovar enteritidis. in high-income settings, it has been responsible for an epidemic of poultry-associated, self-limiting enterocolitis, whereas in sub-saharan africa it is a major cause of invasive nontyphoidal salmonella disease, associated with high case fatality. by whole-genome sequence analysis of 675 isolates of s. enteritidis from 45 countries, we show the existence of a global epidemic clade and two new clades of s. enteritidis t ...201627548315
evaluation of a selected lactic acid bacteria-based probiotic on salmonella enterica serovar enteritidis colonization and intestinal permeability in broiler chickens.two experiments were conducted to evaluate the effect of a lactic acid bacteria-based probiotic (floramax-b11(®)) against salmonella enterica serovar enteritidis intestinal colonization and intestinal permeability in broiler chickens. experiment 1 consisted of two independent trials. in each trial, day-old broiler chicks were assigned to one of two groups: control + s. enteritidis or probiotic + s. enteritidis. at 72 h post-s. enteritidis challenge, haematology and caecal content were evaluated ...201727545145
the antibacterial activity of coriolus versicolor methanol extract and its effect on ultrastructural changes of staphylococcus aureus and salmonella enteritidis.the antibacterial activity of methanol extract obtained from fruiting body of industrially grown basidiomycete coriolus versicolor was examined. the minimum inhibitory concentration (mic) values against various bacteria ranged from 0.625 to 20 mg ml(-1). c. versicolor expressed bactericidal activity against both gram-positive and gram-negative bacteria. the growth curves of staphylococcus aureus and salmonella enterica serovar enteritidis, measured at 630 nm, and confirmed with macrodilution met ...201627540376
salmonella enteritidis isolate harboring multiple efflux pumps and pathogenicity factors, shows absence of o antigen polymerase gene. 201627536269
surveillance of invasive infection in children and adults admitted to qech, blantyre, 1996-2002.this is a report of blood csf isolates from the adults medical and paediatric of wards qech, blantyre, cultured and identified at the welcome trust research laboratories during 1996-2002. the commonest causes of adults and children bacteraemia were non-typhoidal salmonella (35% of all blood isolates for adults and children) and streptococcus pneumoniae (14% and 13% respectively). cryptococcus neoformans was the commonest isolates from csf of adults with meningitis(67%) but was very rare in child ...200327528957
in vitro invasive capacity of salmonella strains into sections of the layer hen oviduct.raw or undercooked eggs and egg products are frequently identified as the source of salmonella following outbreaks of foodborne gastrointestinal disease. some salmonella serovars, such as salmonella enteriditis, have a high tropism for the oviduct of laying hens. oviduct colonization with s. enteriditis can result in both internal and external contamination of an egg. while oviduct invasion is not limited to s. enteriditis, the invasive capacities of other serovars is not widely known. in this s ...201627527775
[investigation of antitumorigenic effects of food-borne non-pathogenic and pathogenic salmonella enterica strains on mef, du145 and hela cell lines].basic applications in cancer therapy may fail to eradicate cancer cells completely, they can show toxic affects to healthy cells and development of resistance to antitumor agents may increase tendency to metastasis. bacterial therapies have the advantage of specific targetting of tumors by selective toxicity, responsiveness to external signals, self-propelling capacity, and the sense of microenvironment. the most interest on the bacterial cancer therapy is about salmonella spp. with a special em ...201627525394
antibacterial and efflux pump inhibitors of thymol and carvacrol against food-borne pathogens.in this study thymol (thy) and carvacrol (car), two monoterpenic phenol produced by various aromatic plants, was tested for their antibacterial and efflux pump inhibitors potencies against a panel of clinical and foodborne pathogenes. our results demonstrated a substantial susceptibility of the tested bacteria toward thy and car. especially, thy displayed a strong inhibitory activity (mic's values ranged from 32 to 64 μg/ml) against the majority of the tested strains compared to car. moreover, a ...201627521228
whole-genome sequencing analysis of salmonella enterica serovar enteritidis isolates in chile provides insights into possible transmission between gulls, poultry, and humans.salmonella enterica subsp. enterica serotype enteritidis is a major cause of human salmonellosis worldwide; however, little is known about the genetic relationships between s enteritidis clinical strains and s enteritidis strains from other sources in chile. we compared the whole genomes of 30 s enteritidis strains isolated from gulls, domestic chicken eggs, and humans in chile, to investigate their phylogenetic relationships and to establish their relatedness to international strains. core geno ...201627520817
efficacy of mealworm and super mealworm larvae probiotics as an alternative to antibiotics challenged orally with salmonella and e. coli infection in broiler chicks.this study was conducted to evaluate dry mealworm (tenebrio molitor) (dmlp) and super mealworm (zophobas morio) (dsmlp) larvae probiotics as alternatives to antibiotics in broiler chicks. a total of 240 one-day old ross 308 male broiler chicks were randomly assigned to three dietary treatments consisting of ten replications with eight birds each in a completely randomized design. the dietary treatments were, (i) control (basal diet), (ii) 0.4% dmlp (basal diet + 0.4% dmlp, dm basis), and (iii) 0 ...201727520069
prevention of egg contamination by salmonella enteritidis after oral vaccination of laying hens with salmonella enteritidis δtolc and δacrabacrefmdtabc mutants.vaccination of laying hens has been successfully used to reduce egg contamination by salmonella enteritidis, decreasing human salmonellosis cases worldwide. currently used vaccines for layers are either inactivated vaccines or live attenuated strains produced by mutagenesis. targeted gene deletion mutants hold promise for future vaccines, because specific bacterial functions can be removed that may improve safety and allow differentiation from field strains. in this study, the efficacy of salmon ...201627519174
chitosan grafted monomethyl fumaric acid as a potential food preservative.the present study aims at in vitro antibacterial and antioxidant activity evaluation of chitosan modified with monomethyl fumaric acid (mfa) using 1-ethyl-3-(3-dimethylaminopropyl) carbodiimide (edc) as mediator. three different kinds of chitosan derivatives ch-ds-1,ch-ds-2 and ch-ds-3 were synthesized by feeding different concentration of mfa. the chemical structures of resulting materials were characterized by (1)h nmr, (13)c nmr, hr-xrd, ft-ir and tnbs assay. the results showed that ch-ds-1, ...201627516253
inactivation of salmonella on eggshells by chlorine dioxide gas.microbiological contamination of eggs should be prevented in the poultry industry, as poultry is one of the major reservoirs of human salmonella. clo2 gas has been reported to be an effective disinfectant in various industry fields, particularly the food industry. the aims of this study were to evaluate the antimicrobial effect of chlorine dioxide gas on two strains of salmonella inoculated onto eggshells under various experimental conditions including concentrations, contact time, humidity, and ...201627499670
effects of salmonella infection on hepatic damage following acute liver injury in rats.acute liver injury is a common clinical disorder associated with intestinal barrier injury and disturbance of intestinal microbiota. probiotic supplementation has been reported to reduce liver injury; however, it is unclear whether enteropathogen infection exacerbates liver injury. the purpose of this study was to address this unanswered question using a rat model.201627498580
evaluation of the antioxidative, antibacterial, and anti-inflammatory effects of the aloe fermentation supernatant containing lactobacillus plantarum hm218749.1.little work is done to develop aloe vera (av) using probiotics. to explore the potential benefits, the antioxidant effects and the antibacterial effects on foodborne pathogens of aloe fermentation supernatant were evaluated in vitro. our results indicated that the aloe fermentation supernatant fermented by lactobacillus plantarum hm218749.1 had very strong scavenging capacities of the dpph (86%), o2 (•-) (85%), (•)oh (76%), and fe(2+) chelation (82%) and reducing powers (242.5 mg/l), and the inh ...201627493450
a curious cause of appendicitis.a previously healthy 10-year-old boy presented to the emergency department with central abdominal pain, loose stool and vomiting. he was diagnosed with gastroenteritis, but was well enough to be discharged. the next day he reattended with ongoing diarrhoea and vomiting, with the pain now localised to the right iliac fossa (rif). acute appendicitis was suspected, and he was taken for laparoscopic appendicectomy. at surgery, a gangrenous appendix was found, with pus extending from the pelvis up to ...201627489071
combination of competitive exclusion and immunisation with a live salmonella vaccine in newly hatched chickens: immunological and microbiological effects.in addition to evaluating the efficacy potential of a combined use of vaccination and competitive exclusion (ce) against salmonella exposure in chicks at 3-days of age, a live salmonella enteritidis vaccine (se-lv) and a ce culture were tested for their ability to induce parameters of the innate immunity. whereas the invasive se-lv induced an influx of granulocytes and macrophages as well as an increased transcription of several cytokines in the caecal mucosa, the ce culture did not demonstrate ...201627473972
chicken-specific kinome array reveals that salmonella enterica serovar enteritidis modulates host immune signaling pathways in the cecum to establish a persistence infection.non-typhoidal salmonella enterica induces an early, short-lived pro-inflammatory response in chickens that is asymptomatic of clinical disease and results in a persistent colonization of the gastrointestinal (gi) tract that transmits infections to naïve hosts via fecal shedding of bacteria. the underlying mechanisms that control this persistent colonization of the ceca of chickens by salmonella are only beginning to be elucidated. we hypothesize that alteration of host signaling pathways mediate ...201627472318
draft genome sequences of salmonella enterica subsp. enterica serovars typhimurium and nottingham isolated from food products.a quantitative real-time pcr (qpcr) designed to detect salmonella enterica subsp. enterica serovar enteritidis, targeting the sdf gene, generated positive results for s. enterica subsp. enterica serovar typhimurium (cfsan033950) and s. enterica subsp. enterica serovar nottingham (cfsan006803) isolated from food samples. both strains show pulsed-field gel electrophoresis (pfge) patterns distinct from those of s enteritidis. here, we report the genome sequences of these two strains.201627445384
capture and concentration of viral and bacterial foodborne pathogens using apolipoprotein h.the need for improved pathogen separation and concentration methods to reduce time-to-detection for foodborne pathogens is well recognized. apolipoprotein h (apoh) is an acute phase human plasma protein that has been previously shown to interact with viruses, lipopolysaccharides (lps) and bacterial proteins. the purpose of this study was to determine if apoh was capable of binding and efficiently capturing two representative human norovirus strains (gi.1 and gii.4), a cultivable surrogate, and f ...201627439140
phytochemical composition and biological activities of selected wild berries (rubus moluccanus l., r. fraxinifolius poir., and r. alpestris blume).berries, from the genus rubus, are among the vital components in a healthy diet. in this study, 80% methanol extracts from the three wild rubus species (rubus moluccanus l., rubus fraxinifolius poir., and rubus alpestris blume) were evaluated for their phytochemical contents (total phenolics, flavonoid, anthocyanin, and carotenoid content), antioxidant (dpph, frap, and abts assays), antiacetylcholinesterase, and antibacterial activities. gc-ms was used for quantification of naturally occurring p ...201627437023
salmonellosis acquired from poultry.among the various poultry product-related foodborne pathogens, gastrointestinal infections caused by egg-borne nontyphoidal salmonella is a major concern in developed and developing countries. this review is focused on the latest findings and implications for food safety.201627434307
salmonellosis associated with mass catering: a survey of european union cases over a 15-year period.salmonella spp. is the causative agent of a foodborne disease called salmonellosis, which is the second most commonly reported gastrointestinal infection in the european union (eu). although over the years the annual number of cases of foodborne salmonellosis within the eu has decreased markedly, in 2014, a total of 88 715 confirmed cases were still reported by 28 eu member states. the european food safety authority reported that, after the household environment, the most frequent settings for t ...201627426630
regulated delayed synthesis of lipopolysaccharide and enterobacterial common antigen of salmonella typhimurium enhances immunogenicity and cross-protective efficacy against heterologous salmonella challenge.lipopolysaccharide (lps) o-antigen and enterobacterial common antigen (eca) are two major polysaccharide structures on the surface of salmonella enterica serovar typhimurium. previous studies have demonstrated that regulated truncation of lps enhances the cross-reaction against conserved outer membrane proteins (omps) from enteric bacteria. we speculate that the regulation of both o-antigen and eca may enhance the induction of immune responses against conserved omps from enteric bacteria. in thi ...201627423383
nontyphoidal salmonellosis, human immunodeficiency virus infection, and ischemic stroke.nontyphoidal salmonella infection and stroke are major causes of morbidity and mortality worldwide, with increased risk in the human immunodeficiency virus (hiv)-infected population. we report a rare case of ischemic stroke associated with salmonella enteritidis subdural empyema in an older hiv-infected patient with multimorbidity, despite surgery and treatment with susceptible antimicrobial drugs.201627419176
bactericidal activity of tigemonam, alone and in combination with gentamicin.the in vitro bactericidal activity of tigemonam, alone and in combination with gentamicin, was evaluated against various strains of escherichia coli, klebsiella pneumoniae, klebsiella oxytoca, salmonella enteritidis, enterobacter cloacae, proteus mirabilis, and proteus vulgaris. minimum inhibitory and bactericidal concentrations were determined on three consecutive days with an inoculum of 10(5) colony-forming units (cfu)/ml or a culture in the logarithmic growth phase. the results found tigemon ...198927416150
[clinical epidemiology and antimicrobial resistance of nontyphoidal salmonella enteric infections in children: 2012-2014].to understand the clinical epidemiology and manifestation of nontyphoidal salmonella (nts)enteric infections and serotypes and antibiotic resistance patterns of nts in children.201627412737
4-hydroxy-3-nitrophenylacetic and sinapic acids as antibacterial compounds from mustard seeds.a methanol extract from yellow mustard seeds had antibacterial activity against escherichia coli, salmonella enteritidis, and staphylococcus aureus. two compounds with such activity were isolated from the extract. by instrumental analysis, the compounds were identified as 4-hydroxy-3-nitrophenylacetic and sinapic acids. examination of the structure-activity relationship showed that the hydroxyl and nitro groups of the first compound were involved in the activity against all three species. the tw ...199827392592
salmonella enterica serovar enteritidis brain abscess mimicking meningitis after surgery for glioblastoma multiforme: a case report and review of the literature.salmonella brain abscess associated with brain tumor is rare. only 11 cases have been reported to date. here we report a case of brain abscess caused by salmonella enterica serovar enteritidis mimicking post-surgical meningitis in a patient with glioblastoma multiforme.201627387824
composition of gut microbiota influences resistance of newly hatched chickens to salmonella enteritidis infection.since poultry is a very common source of non-typhoid salmonella for humans, different interventions aimed at decreasing the prevalence of salmonella in chickens are understood as an effective measure for decreasing the incidence of human salmonellosis. one such intervention is the use of probiotic or competitive exclusion products. in this study we tested whether microbiota from donor hens of different age will equally protect chickens against salmonella enteritidis infection. newly hatched chic ...201627379083
salmosan, a β-galactomannan-rich product, protects epithelial barrier function in caco-2 cells infected by salmonella enterica serovar enteritidis.one promising strategy for reducing human salmonellosis induced by salmonella enteritidis is to supplement animal diets with natural feed additives such as mannan oligosaccharides (moss).201627358412
alternative antimicrobial commercial egg washing procedures.commercial table eggs are washed prior to packaging. standard wash procedures use an alkaline ph and warm water. if a cool water method could be developed that would still provide a microbiologically safe egg, the industry may save energy costs associated with water heating. four wash procedures were evaluated for salmonella reduction: ph 11 at 48.9°c (industry standard), ph 11 at ambient temperature (∼20°c), ph 6 at 48.9°c, and ph 6 at ambient temperature. alkaline washes contained potassium hy ...201627357042
inactivation of salmonella on sprouting seeds using a spontaneous carvacrol nanoemulsion acidified with organic acids.over the past decade, demand has increased for natural, minimally processed produce, including sprout-based products. sanitization with 20,000 ppm of calcium hypochlorite is currently recommended for all sprouting seeds before germination to limit sprout-related foodborne outbreaks. a potentially promising disinfectant as an alternative to calcium hypochlorite is acidified spontaneous essential oil nanoemulsions. in this study, the efficacy of an acidified carvacrol nanoemulsion was tested again ...201627357030
study on the mechanism of antibacterial action of magnesium oxide nanoparticles against foodborne pathogens.magnesium oxide nanoparticles (mgo nanoparticles, with average size of 20 nm) have considerable potential as antimicrobial agents in food safety applications due to their structure, surface properties, and stability. the aim of this work was to investigate the antibacterial effects and mechanism of action of mgo nanoparticles against several important foodborne pathogens.201627349516
o-antigen chain-length distribution in salmonella enterica serovar enteritidis is regulated by oxygen availability.lipopolysaccharide (lps) consists of three covalently linked domains: the lipid a, the core region and the o antigen (oag), consisting of repeats of an oligosaccharide. salmonella enterica serovar enteritidis (s. enteritidis) produces a lps with two oag preferred chain lengths: a long (l)-oag controlled by wzzse and a very long (vl)-oag controlled by wzzfepe. in this work, we show that oag produced by s. enteritidis grown in e minimal medium also presented two preferred chain-lengths. however, a ...201627343553
corrigendum to "evaluation of a salmonella enteritidis vaccine and related elisa for respective induction and assessment of acquired immunity to the vaccine and/or echinacea purpurea in awassi ewes" [vaccine 33 (2015) 2228-2231]. 201627329183
physical and antimicrobial properties of cinnamon bark oil co-nanoemulsified by lauric arginate and tween 80.lauric arginate (lae) is a water-soluble cationic surfactant which has antimicrobial activity against a broad spectrum of foodborne pathogens. some spice essential oils are effective lipophilic antimicrobials. combining both antimicrobials may reduce their usage levels and possible negative sensory impacts when applied in complex food matrices. the objective of this study was to combine a nonionic surfactant (tween 80) with lae to form stable nanoemulsions with cinnamon bark essential oil (cbo) ...201627322724
prevalence and antibiotic resistance of bacterial pathogens isolated from childhood diarrhea in beijing, china (2010-2014).diarrhea is one of the main causes of morbidity and mortality among children less than 5 years of age worldwide, and its causes vary by region. this study aimed to determine the etiologic spectrum, prevalent characteristics and antimicrobial resistance patterns of common enteropathogenic bacteria from diarrheagenic children in beijing, the capital of china.201627303446
enhanced protective immunity of the chimeric vector-based vaccine radv-sfv-e2 against classical swine fever in pigs by a salmonella bacterial ghost adjuvant.classical swine fever (csf) is a highly contagious swine disease caused by classical swine fever virus (csfv). previously, we demonstrated that radv-sfv-e2, an adenovirus-delivered, semliki forest virus replicon-vectored marker vaccine against csf, is able to protect pigs against lethal csfv challenge. from an economical point of view, it will be beneficial to reduce the minimum effective dose of the vaccine. this study was designed to test the adjuvant effects of salmonella enteritidis-derived ...201627301745
pleural empyema caused by salmonella enteritidis in a patient with non-hodgkin lymphoma.extraintestinal manifestations of nontyphoidal salmonellosis are usually seen in patients with cellular immunodeficiency. pleural empyema caused by nontyphoidal salmonella is very rare clinical presentation of salmonellosis and there are just a few cases described in a literature. we presented a very rare case of pleural empyema caused by salmonella enteritidis in a patient with non-hodgkin limphoma.201627295915
transcriptional changes of cytokines in rooster testis and epididymis during sexual maturation stages and salmonella infection.infection of rooster testis and epididymis by pathogens can lead to impaired fertility, resulting in economic losses in the poultry industry. antimicrobial protection of rooster reproductive organs is, therefore, an important aspect of reproductive physiology. salmonellosis is one of the most important zoonotic diseases, caused by salmonella bacteria including salmonella enteritidis (se) and is usually the result of infection of the reproductive organs. thus, knowledge of the endogenous innate i ...201627289435
microbiological, clinical and molecular findings of non-typhoidal salmonella bloodstream infections associated with malaria, oriental province, democratic republic of the congo.in sub-saharan africa, non-typhoidal salmonella (nts) can cause bloodstream infections, referred to as invasive non-typhoidal salmonella disease (ints disease); it can occur in outbreaks and is often preceded by malaria. data from central africa is limited.201627286886
a live attenuated salmonella enteritidis secreting detoxified heat labile toxin enhances mucosal immunity and confers protection against wild-type challenge in chickens.a live attenuated salmonella enteritidis (se) capable of constitutively secreting detoxified double mutant escherichia coli heat labile toxin (dmlt) was developed. the biologically adjuvanted strain was generated via transformation of a highly immunogenic se jol1087 with a plasmid encoding dmlt gene cassette; the resultant strain was designated jol1641. a balanced-lethal host-vector system stably maintained the plasmid via auxotrophic host complementation with a plasmid encoded aspartate semiald ...201627262338
spi-9 of salmonella enterica serovar typhi is constituted by an operon positively regulated by rpos and contributes to adherence to epithelial cells in culture.the genomic island 9 (spi-9) from salmonella enterica serovar typhi (s. typhi) carries three orfs (sty2876, sty2877, sty2878) presenting 98 % identity with a type 1 secretory apparatus (t1ss), and a single orf (sty2875) similar to a large rtx-like protein exhibiting repeated ig domains. bapa, the salmonella enterica serovar enteritidis orthologous to s. typhi sty2875, has been associated with biofilm formation, and is described as a virulence factor in mice. preliminary in silico analyses reveal ...201627260307
primary sternal osteomyelitis caused by salmonella enteritidis. 201727256249
serotype epidemiology and multidrug resistance patterns of salmonella enterica infecting humans in italy.salmonella enterica is the zoonotic agent most frequently responsible for foodborne infections in humans worldwide. in this work the presence of s. enterica was investigated in 734 unique enteropathogenic isolates collected from human patients between 2011 and 2012.201627252785
inactivation of salmonella enteritidis on shell eggs by coating with phytochemicals.salmonella enteritidis (se) is a major foodborne pathogen that causes human infections largely by consumption of contaminated eggs. the external surface of eggs becomes contaminated with se from multiple sources, highlighting the need for effective egg surface disinfection methods. this study investigated the efficacy of three gras-status, phytochemicals, namely carvacrol (cr), eugenol (eg), and β-resorcylic acid (br) applied as pectin or gum arabic based coating for reducing se on shell eggs. w ...201627252373
survey of the prevalence of salmonella species on laying hen farms in kosovo.a survey on the prevalence of salmonella (s) species was carried out on 39 layer farms in kosovo between april and september 2012. in total 367 samples, comprising feces, dust, eggs, and internal organs from dead birds, were investigated using bacteriological culture methods. additionally, data on the location of the farm, the total number of birds on the farm, age of birds, and laying performance were collected. salmonella were isolated from 38 samples obtained from 19 (49%) farms. the most com ...201627252368
the application of alkaline and acidic electrolyzed water in the sterilization of chicken breasts and beef liver.the sterilization effect of a combination treatment with alkaline electrolyzed water (alew) and strong acidic electrolyzed water (staew) on fresh chicken breasts and beef liver was evaluated. samples (1, 5, and 10 g) were inoculated with salmonella enteritidis nbrc3313, escherichia coli atcc 10798, staphylococcus aureus fda209p, and s. aureus c-29 [staphylococcal enterotoxin a (sea) productive strain] and subjected to a dipping combination treatment (4°c and 25°c for 3 min) with alew and staew. ...201627247773
salmonella contamination, serovars and antimicrobial resistance profiles of cattle slaughtered in south africa.antimicrobial resistant salmonella are among the leading causes of foodborne infections. our aim was to determine salmonella contamination during cattle slaughter in south african rural abattoirs (n = 23) and environmental samples. furthermore, antimicrobial resistance patterns of the salmonella isolates were determined. samples of cattle faeces (n = 400), carcass sponges (n = 100), intestinal contents (n = 62), hides (n = 67), and water from the abattoirs (n = 75) were investigated for salmonel ...201627247074
validation of the reveal(®) 2.0 group d1 salmonella test for detection of salmonella enteritidis in raw shell eggs and poultry-associated matrixes.a study was conducted to assess the performance of the reveal(®) 2.0 group d1 salmonella lateral flow immunoassay for use in detection of salmonella enteritidis (se) in raw shell eggs and poultry-associated matrixes, including chicken carcass rinse and poultry feed. in inclusivity testing, the reveal 2.0 test detected all 37 strains of se tested. the test also detected all but one of 18 non-enteritidis somatic group d1 salmonella serovars examined. in exclusivity testing, none of 42 strains test ...201627214854
disinfectant susceptibility of different salmonella serotypes isolated from chicken and egg production chains.the study aimed to serotype the salmonella isolates recovered from chicken and egg production chains, and to investigate the disinfectant resistance phenotypes and genotypes of these isolates.201627206326
characterization of a highly potent antimicrobial peptide microcin n from uropathogenic escherichia coli.microcin n is a low-molecular weight, highly active antimicrobial peptide produced by uropathogenic escherichia coli in this study, the native peptide was expressed and purified from pgob18 plasmid carrying e. coli in low yield. the pure peptide was characterized using mass spectrometry, n-terminal sequencing by edman degradation as well as trypsin digestion. we found that the peptide is 74-residue long, cationic (+2 total charge), highly hydrophobic and consists of glycine as the first n-termin ...201627190283
evaluation of moringa oleifera seed lectin as a metal remover in aqueous solutions.the efficacy of moringa oleifera seed lectin (wsmol) as a metal remover in water and the effects of metals on its hemagglutinating and antibacterial activities were determined. aqueous metal solutions were incubated with wsmol for 8 h at 4°c and the concentrations of metals were determined using atomic absorption spectroscopy. hemagglutination and antibacterial assays were conducted with wsmol and lectin exposed or not to the metals. the removal efficiency of wsmol was 49.00%, 53.21%, 71.45%, 55 ...201627184499
whole-genome sequence of salmonella enterica serovar enteritidis phage type 4, isolated from a brazilian poultry farm.the draft genome of salmonella enterica serovar enteritidis phage type 4 (pt4) strain ioc4647/2004, isolated from a poultry farm in são paulo state, was obtained with high-throughput illumina sequencing platform, generating 4,173,826 paired-end reads with 251 bp. the assembly of 4,804,382 bp in 27 scaffolds shows strong similarity to other s enteritidis strains.201627174265
use of lactobacillus plantarum strains as a bio-control strategy against food-borne pathogenic microorganisms.lactobacillus plantarum is one of the most versatile species extensively used in the food industry both as microbial starters and probiotic microorganisms. several l. plantarum strains have been shown to produce different antimicrobial compounds such as organic acids, hydrogen peroxide, diacetyl, and also bacteriocins and antimicrobial peptides, both denoted by a variable spectrum of action. in recent decades, the selection of microbial molecules and/or bacterial strains able to produce antagoni ...201627148172
activation of chicken bone marrow-derived dendritic cells induced by a salmonella enteritidis ghost vaccine candidate.bacterial ghost-based vaccine development has been applied to a variety of gram-negative bacteria. developed salmonella enteritidis (s. enteritidis) ghost are promising vaccine candidates because of their immunogenic and enhanced biosafety potential. in this study, we aimed to evaluate the immunostimulatory effect of a s. enteritidis ghost vaccine on the maturation of chicken bone marrow-derived dendritic cells (chbm-dcs) in vitro the immature chbm-dcs were stimulated with s. enteritidis ghost v ...201627143780
pericardial salmonella with cardiac tamponade and ventricular wall rupture: a case report.non-typhoidal salmonella (nts) is mostly restricted to gastroenteritis; however, we report a case of salmonella pericarditis complicated by tamponade and spontaneous ventricular wall rupture.201627141304
the salmonella pathogenicity island 13 contributes to pathogenesis in streptomycin pre-treated mice but not in day-old chickens.salmonella enterica serovar enteritidis (s. enteritidis) is a human and animal pathogen that causes gastroenteritis characterized by inflammatory diarrhea and occasionally an invasive systemic infection. salmonella pathogenicity islands (spis) are horizontally acquired genomic segments known to contribute to salmonella pathogenesis. the objective of the current study was to determine the contribution of spi-13 to s. enteritidis pathogenesis.201627141235
altered virulence potential of salmonella enteritidis cultured in different foods: a cumulative effect of differential gene expression and immunomodulation.salmonella enterica serovars enteritidis (s. enteritidis) is one of the most common causes of food borne illness. bacterial growth environment plays an important role in regulating gene expression thereby affecting the virulence profile of the bacteria. different foods present diverse growth conditions which may affect the pathogenic potential of the bacteria. in the present study, the effect of food environments on the pathogenic potential of s. enteritidis has been evaluated. s. enteritidis wa ...201627132148
emergence of host-adapted salmonella enteritidis through rapid evolution in an immunocompromised host.host adaptation is a key factor contributing to the emergence of new bacterial, viral and parasitic pathogens. many pathogens are considered promiscuous because they cause disease across a range of host species, while others are host-adapted, infecting particular hosts(1). host adaptation can potentially progress to host restriction where the pathogen is strictly limited to a single host species and is frequently associated with more severe symptoms. host-adapted and host-restricted bacterial cl ...201627127642
prevalence and antibiotic resistance of salmonella enteritidis and salmonella typhimurium in raw chicken meat at retail markets in malaysia.salmonellosis is one of the major food-borne diseases in many countries. this study was carried out to determine the occurrence of salmonella spp., salmonella enteritidis, and salmonella typhimurium in raw chicken meat from wet markets and hypermarkets in selangor, as well as to determine the antibiotic susceptibility profile of s. enteritidis and s. typhimurium. the most probable number (mpn) in combination with multiplex polymerase chain reaction (mpcr) method was used to quantify the salmonel ...201627118863
thoracic spinal osteomyelitis due to salmonella enteritidis in an immunocompetent mimicking tuberculosis. 201727114675
observations on the distribution and control of salmonella in commercial duck hatcheries in the uk.salmonella infection causes a significant number of cases of gastroenteritis and more serious illnesses in people in the uk and eu. the serovars salmonella enteritidis and salmonella typhimurium are most frequently associated with foodborne illness in europe. whilst control programmes exist to monitor these serovars in the chicken and turkey sectors, no regulatory programme is currently in place for the duck sector. a voluntary industry scheme (duck assurance scheme) was launched in the uk in 20 ...201627100154
assessment of potentially probiotic properties of lactobacillus strains isolated from chickens.this study was performed in order to isolate lactobacilli from chicken droppings and to select strains with the most promising probiotic properties. lactobacillus strains were isolated from a flock of healthy laying hens. the first selection criterion was the ability to inhibit the growth of salmonella enteritidis. then the tolerance to low ph and bile salt, the ability to coaggregate with pathogenic bacteria and hydrogen peroxide production were evaluated. four isolates showing the best antagon ...201627096783
effect of polymer/nanosilver composite packaging on long-term microbiological status of iranian saffron (crocus sativus l.).crocus sativus l. (saffron) is a valuable plant which is native to iran. saffron is the dried stigmata of the flowering part of the plant that is usually contaminated with different bacteria and fungi through production process. antimicrobial properties of silver nanoparticles are well recognized. to survey the effects of nanosilver packaging on microbiological status of spiked, saffron samples over a six month period were chosen. saffron samples from five regions of khorasan province were purch ...201627081358
red seaweeds sarcodiotheca gaudichaudii and chondrus crispus down regulate virulence factors of salmonella enteritidis and induce immune responses in caenorhabditis elegans.red seaweeds are a rich source of unique bioactive compounds and secondary metabolites that are known to improve human and animal health. s. enteritidis is a broad range host pathogen, which contaminates chicken and poultry products that end into the human food chain. worldwide, salmonella outbreaks have become an important economic and public health concern. moreover, the development of resistance in salmonella serovars toward multiple drugs highlights the need for alternative control strategie ...201627065981
evaluation of potential probiotic characteristics and antibacterial effects of strains of pediococcus species isolated from broiler chickens.pediococcus spp. were isolated from poultry rectum, faeces and food as good probiotic candidates in order to select strains to be used as probiotic in poultry feed. a total of 168 lactic acid bacteria were isolated and 51 isolates including 31 lactobacillus spp. and 20 pediococcus spp. were able to survive in low ph and bile salt concentration. the pediococcus spp. were identified and their ability to form biofilm, adhesion to caco-2 cells and antimicrobial activities against enteric pathogenic ...201627057800
evaluation the antibacterial effects of echinophora platyloba extracts against some salmonella species.salmonellosis, which is caused by nontyphoid salmonella bacteria, is one of the most common foodborne diseases, and it causes gastrointestinal infections worldwide, most of which are limited gastroenteritis that requires antimicrobial treatment. the aim of this study was to investigate the effects of echinophora platyloba extract on inhibiting the growth of salmonella typhi, salmonella enteritidis, and salmonella choleraesuis.201627054002
isolation and identification of salmonella from diarrheagenic infants and young animals, sewage waste and fresh vegetables.this study was carried out to determine the prevalence, distribution, and identification of salmonella serotypes in diarrheagenic infants and young animals, including sewage waste and fresh vegetables.201527047154
detection of salmonella spp. from chevon, mutton and its environment in retail meat shops in anand city (gujarat), india.the aim of this study was (i) to attempt isolation and identification of salmonella species from samples. (ii) serotyping of salmonella isolates. (iii) detection of virulence factor associated genes by polymerase chain reaction (pcr).201527047102
the commercial impact of pig salmonella spp. infections in border-free markets during an economic recession.the genus salmonella, a group of important zoonotic pathogens, is having global economic and political importance. its main political importance results from the pathogenicity of many of its serovars for man. serovars salmonella enteritidis and salmonella typhimurium are currently the most frequently associated to foodborne infections, but they are not the only ones. animal food products contaminated from subclinically infected animals are a risk to consumers. in border free markets, an example ...201527047083
prevalence and epidemiology of salmonella enterica serovar gallinarum from poultry in some parts of haryana, india.the present study was investigated to ascertain the epidemiological status of fowl typhoid (ft) in broilers in some parts of haryana during january 2011 to december 2013.201527047033
salmonella typhimurium and outbreaks of egg-associated disease in australia, 2001 to 2011.salmonellosis is a significant public health problem, with eggs frequently identified as a food vehicle during outbreak investigations. salmonella enterica serovar typhimurium and salmonella enterica serovar enteritidis are the two most frequently identified causes of egg-associated disease in industrialized countries. in australia, a comprehensive review of egg-associated outbreaks has not been previously undertaken.201627028267
functional characterization of glucosamine-6-phosphate synthase (glms) in salmonella enterica serovar enteritidis.salmonella is a threat to public health due to consumption of contaminated food. screening of a transposon library identified a unique mutant that was growth and host cell binding deficient. the objective of this study was to determine the functional role of glucosamine-6-phosphate synthase (glms) in the biology and pathogenesis of salmonella. to examine this, we created a glms mutant (δglms) of salmonella and examined the effect on cell envelope integrity, growth, metabolism, and pathogenesis. ...201627017337
serovars and antimicrobial resistance of salmonella spp. isolated from turkey and broiler carcasses in southern brazil between 2004 and 2006.salmonella spp. causes diseases in fowls, when species-specific serovars (salmonella pullorum and s.gallinarum) are present in flocks, and public health problems, when non-typhoid serovars are isolated, as well as possible bacterial resistance induced by the preventive and therapeutic use of antimicrobials in animal production. this study describes the serovars and bacterial resistance of 280 salmonella spp. strains isolated from turkey and broiler carcasses in southern brazil between 2004 and 2 ...201627007562
integration of antimicrobial pectin-based edible coating and active modified atmosphere packaging to preserve the quality and microbial safety of fresh-cut persimmon (diospyros kaki thunb. cv. rojo brillante).the greatest hurdle to the commercial marketing of fresh-cut fruits is related to their higher susceptibility to enzymatic browning, tissue softening, and microbial growth. the aim of this study was to test the efficacy of a pectin-based edible coating and low oxygen modified atmosphere packaging (map) to control enzymatic browning and reduce microbial growth of fresh-cut 'rojo brillante' persimmon. the survival of escherichia coli, salmonella enteritidis and listeria monocytogenes artificially ...201626997097
microbial-resistant salmonella enteritidis isolated from poultry samples.multidrug resistance in salmonella enteritidis isolates is a public health problem worldwide; the present study, therefore, was designed for antimicrobial-resistance determination in this strain.201226989702
[characteristics of drug resistance and molecular typing research for salmonella enteritidis isolated in henan province from 2011 to 2013].to investigate the antimicrobial resistance status and pulsed field gel electrophoresis (pfge) patterns of salmonella enteritidis (s.enteritidis) strains in henan province.201626957246
the impact of salmonella enteritidis on lipid accumulation in chicken hepatocytes.salmonella enterica serovar enteritidis (se) is a public health concern and infected chickens serve as a reservoir that potentially transmits to humans through food. although se seldom causes systemic disease in chickens, virulent se strains can colonize in intestines and lead a persistent infection of the liver. the liver is the primary organ for lipid metabolism in chickens and the site for production and assembly of main components in yolk. we performed a time-course experiment using lmh-2a c ...201626957042
the antibiofilm effect of ginkgo biloba extract against salmonella and listeria isolates from poultry.salmonella spp. and listeria spp. are common foodborne pathogens in poultry and have caused a large number of outbreaks worldwide. biofilm formation is common in the food industry and is also a mechanism of antimicrobial resistance. the aim of this work was to investigate the antimicrobial effect and mechanism of ginkgo biloba extract against the biofilm formation of salmonella and listeria isolates from poultry at retail markets. bacteria detection, isolation, and enumeration were carried out o ...201626954614
characterization of seven outbreaks of hemorrhagic hepatopathy syndrome in commercial pullets following the administration of a salmonella enteritidis bacterin in california.between april 2013 and april 2015, seven flocks belonging to three different major commercial egg producers incalifornia experienced a mild increase in mortality 2 to 3 wk after administration of salmonella enteritidis bacterins. strains of chickens involved were h&n (flock a1, a2, b2, c1, c2, and c3) and lohmann white (flock b1). vaccination was administered individually through injection either in the breast muscles or subcutis in the legs between 11 and 18 wk of age in all flocks. clinical si ...201626953941
highly expressed amino acid biosynthesis genes revealed by global gene expression analysis of salmonella enterica serovar enteritidis during growth in whole egg are not essential for this growth.salmonella enterica serovar enteritidis (s. enteritidis) is the most common cause of egg borne salmonellosis in many parts of the world. this study analyzed gene expression of this bacterium during growth in whole egg, and whether highly expressed genes were essential for the growth. high quality rna was extracted from s. enteritidis using a modified rna-extraction protocol. global gene expression during growth in whole egg was compared to growth in lb-medium using dna array method. twenty-six g ...201626945769
colonization of internal organs by salmonella enteritidis in experimentally infected laying hens housed in enriched colony cages at different stocking densities.epidemiologic analyses have linked the frequency of human infections with salmonella enterica subspecies enterica serovar enteritidis to the consumption of contaminated eggs and thus to the prevalence of this pathogen in commercial egg-laying flocks. contamination of the edible contents of eggs by salmonella enteritidis is a consequence of the colonization of reproductive tissues in systemically infected hens. the animal welfare implications of laying hen housing systems have been widely debated ...201626944976
comparison of eggshell surface sanitization technologies and impacts on consumer acceptability.shell eggs can be contaminated with many types of microorganisms, including bacterial pathogens, and thus present a risk for the transmission of foodborne disease to consumers. currently, most united states egg processors utilize egg washing and sanitization systems to decontaminate surfaces of shell eggs prior to packaging. however, previous research has indicated that current shell egg sanitization technologies employed in the commercial egg industry may not completely eliminate bacteria from ...201626944973
study of salmonella typhimurium infection in laying hens.members of salmonella enterica are frequently involved in egg and egg product related human food poisoning outbreaks worldwide. in australia, salmonella typhimurium is frequently involved in egg and egg product related foodborne illness and salmonella mbandaka has also been found to be a contaminant of the layer farm environment. the ability possessed by salmonella enteritidis to colonize reproductive organs and contaminate developing eggs has been well-described. however, there are few studies ...201626941727
efficacy of traditional almond decontamination treatments and electron beam irradiation against heat-resistant salmonella strains.two outbreaks of salmonellosis were linked to the consumption of raw almonds from california in 2001 and 2004. as a result, federal regulations were developed, which mandate that all almonds grown in california must be treated with a process that results in a 4-log reduction of salmonella. because most of the technologies approved to treat almonds rely on the application of heat to control salmonella, an evaluation of alternative technologies for inactivating heat-resistant salmonella enteritidi ...201626939646
prevalence, level, and types of salmonella isolated from north american in-shell pecans over four harvest years.in-shell pecan samples (500 g) were collected over four harvest seasons (2010 to 2014) from seven pecan shelling facilities located in five u.s. states. four varieties of pecans were analyzed: mexican improved, native seedlings, southern improved, and western improved. pecan samples (100 g) were sent to a third party laboratory for initial salmonella screening. when a sample was positive for salmonella, the pathogen level was determined by the most-probable-number (mpn) method (25, 2.5, and 0.25 ...201626939644
growth in egg yolk enhances salmonella enteritidis colonization and virulence in a mouse model of human colitis.salmonella enteritidis (se) is one of the most common causes of bacterial food-borne illnesses in the world. despite the se's ability to colonize and infect a wide-range of host, the most common source of infection continues to be the consumption of contaminated shell eggs and egg-based products. to date, the role of the source of se infection has not been studied as it relates to se pathogenesis and resulting disease. using a streptomycin-treated mouse model of human colitis, this study examine ...201626939126
validation and identification of invasive salmonella serotypes in sub-saharan africa by multiplex polymerase chain reaction.salmonella enterica serovar typhi and nontyphoidal salmonella (nts) cause the majority of bloodstream infections in sub-saharan africa; however, serotyping is rarely performed. we validated a multiplex polymerase chain reaction (pcr) assay with the white-kauffmann-le minor (wklm) scheme of serotyping using 110 salmonella isolates from blood cultures of febrile children in ghana and applied the method in other typhoid fever surveillance in africa program study sites. in ghana, 47 (43%) s. typhi, ...201626933026
the emergence of reduced ciprofloxacin susceptibility in salmonella enterica causing bloodstream infections in rural ghana.salmonella ranks among the leading causes of bloodstream infections in sub-saharan africa. multidrug resistant typhoidal and nontyphoidal salmonella (nts) isolates have been previously identified in this region. however, resistance to ciprofloxacin has rarely been reported in west africa. this study aims to assess susceptibility against ciprofloxacin in salmonella causing invasive bloodstream infections among children in rural ghana.201626933017
the relationship between invasive nontyphoidal salmonella disease, other bacterial bloodstream infections, and malaria in sub-saharan africa.country-specific studies in africa have indicated that plasmodium falciparum is associated with invasive nontyphoidal salmonella (ints) disease. we conducted a multicenter study in 13 sites in burkina faso, ethiopia, ghana, guinea-bissau, kenya, madagascar, senegal, south africa, sudan, and tanzania to investigate the relationship between the occurrence of ints disease, other systemic bacterial infections, and malaria.201626933016
celiac crisis in children in serbia.to assess the prevalence and risk factors of celiac crisis (cc) in children with classical celiac disease (cd).201626931303
inhibition of enteropathogens adhesion to human enterocyte-like ht-29 cells by a dairy strain of propionibacterium acidipropionici.adhesion to the host intestinal mucosa is considered relevant for orally delivered probiotics as it prolongs their persistence in the gut and their health promoting effects. classical propionibacteria are microorganisms of interest due to their role as dairy starters as well as for their functions as probiotics. propionibacterium acidipropionici q4, is a dairy strain isolated from a swiss-type cheese made in argentina that displays probiotic potential. in the present work we assessed the ability ...201626925604
prophylactic administration of chicken cathelicidin-2 boosts zebrafish embryonic innate immunity.chicken cathelicidin-2 (cath-2) is a host defense peptide that exhibits immunomodulatory and antibacterial properties. here we examined effects of cath-2 in zebrafish embryos in the absence and presence of infection. yolk-injection of 0.2-1.5 h post-fertilized (hpf) zebrafish embryos with 2.6 ng/kg cath-2 increased proliferation of phagocytic cells at 48 hpf by 30%. a lethal infection model was developed to test the prophylactic protective effect of cath-2 peptide. embryos (0.2-1.5 hpf) were inj ...201626920462
the effect of oregano essential oil on microbial load and sensory atributes of dried meat.microbial load can be controlled using either synthetic or natural preservatives. particular interest has been focused on the potential application of plant essential oils as safer additives for meat. however, there is no applied research about the use of essential oils during the meat drying process. this study is focused on enhancing the meat drying process, a value-added dried meat product by using oregano essential oil (oeo) to inhibit the growth of bacteria, and the sensorial response from ...201626917258
comparing the ability of luminex xmap(®) salmonella serotyping assay and traditional serotyping method for serotyping salmonella isolated from southern chinese population.to investigate whether the luminex xmap(®) salmonella serotyping assay (xmap ssa) is applicable to serotype salmonella from humans in southern china.201626914944
genetic resistance to mortality of day-old chicks and carrier-state of hens after inoculation with salmonella enteritidis.the heritability of resistance of poultry to salmonella enteritidis (se) was investigated. three measurements of resistance were made: survival after intramuscular inoculation of 419 day-old chicks, absence versus presence of salmonella in spleens and caeca 4 weeks after oral inoculation of 304 hens at peak of laying, and antibody response of 228 hens following two inoculations of an aroa mutant of this serotype. in the first two models of infection, resistance appeared to be heritable. the heri ...199926911499
screening of the enterocin-encoding genes and antimicrobial activity in enterococcus species.in the current study, a total of 135 enterococci strains from different sources were screened for the presence of the enterocin-encoding genes enta, entp, entb, entl50a, and entl50b. the enterocin genes were present at different frequencies, with enta occurring the most frequently, followed by entp and entb; entl50a and l50b were not detected. the occurrence of single enterocin genes was higher than the occurrence of multiple enterocin gene combinations. the 80 isolates that harbor at least one ...201626907753
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