Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| rnai knockdown of acetyl-coa carboxylase gene eliminates jinggangmycin-enhanced reproduction and population growth in the brown planthopper, nilaparvata lugens. | a major challenge in ecology lies in understanding the coexistence of intraguild species, well documented at the organismal level, but not at the molecular level. this study focused on the effects of the antibiotic, jinggangmycin (jgm), a fungicide widely used in asian rice agroecosystems, on reproduction of insects within the planthopper guild, including the brown planthopper (bph) nilaparvata lugens and the white-backed planthopper (wbph) sogatella furcifera, both serious resurgence rice pests ... | 2015 | 26482193 |
| intraspecific and interspecific variations in the mitochondrial genomes of nilaparvata (hemiptera: delphacidae). | planthoppers in the genus nilaparvata distant are serious pests of rice and many other crops in tropical and temperate asia, and northern australia. in this study, the mitochondrial genomes of four nilaparvata planthoppers were sequenced, three in nilaparvata lugens stål and one in nilaparvata muiri china. mitochondrial genome of nilaparvata contain the standard set of 13 protein-coding genes, 22 transfer rna genes, 2 ribosomal rna genes, and a control region. the nucleotide composition of nilap ... | 2015 | 26470349 |
| analysis of the time-concentration-mortality response of nilaparvata lugens (hemiptera: delphacidae) to paichongding. | paichongding is an adicyclicnitromethylene neonicotinoid insecticide with a cis-configuration. bioassay of paichongding was conducted against nilaparvata lugens stål under a laboratory condition. mortality of n. lugens was analyzed by time-concentration-mortality (tcm) regressions based on the complementary log-log (cll) model. the conditional mortalities of test individuals increased with the exposure time after treatments with different concentrations, showing that the speed of insecticidal ac ... | 2015 | 26470347 |
| a comparative study on the population fitness of three strains of nilaparvata lugens (hemiptera: delphacidae) differ in eye color-related genes. | the brown planthopper, nilaparvata lugens (stål), is a destructive insect pest on rice throughout asia. as a visible genetic marker, red eye mutant colony of brown planthopper is a valuable material. here, we established the near-isogenic lines, nil-bb and nil-rr, through mating red eye females to brown eye brothers for eight successive generations. biological experiments showed that nil-bb had big fitness cost; however, nil-rr had comparable survival and fertility parameters with bb, a normal l ... | 2015 | 26470308 |
| infection rate assay by nested pcr and the phylogenetic analysis of himetobi p virus in the main pests of rice-wheat cropping systems. | himetobi p virus (hipv) is an ssrna in the family dicistroviridae that infects rice pests belonging to hemiptera. to determine its host range, a nested pcr method was designed to detect hipv in some of the main rice pests (hemiptera) in eastern china. the incidence of infection in the grain aphid sitobion avenae fabricius (hemiptera: aphididae) was low (3%), while high incidences of infection occurred in the planthoppers laodelphax striatellus (fallén) (hemiptera: delphacidae) (100%) and nilapar ... | 2015 | 26470259 |
| effects of host sex, plant species, and putative host species on the prevalence of wolbachia in natural populations of bemisia tabaci (hemiptera: aleyrodidae): a modified nested pcr study. | bemisia tabaci (gennadius) (hemiptera: aleyrodidae) is a globally distributed pest. one of the key endosymbionts in b. tabaci is wolbachia, an α-proteobacterium implicated in many important biological processes. previous studies indicated that the infection frequency of wolbachia in middle east-asia minor 1 (meam1) and mediterranean (med) varied greatly among populations in different areas. however, little is known about the factors that influence the prevalence of wolbachia in b. tabaci. in thi ... | 2015 | 26470122 |
| jasmonic acid carboxyl methyltransferase regulates development and herbivory-induced defense response in rice. | jasmonic acid (ja) and related metabolites play a key role in plant defense and growth. ja carboxyl methyltransferase (jmt) may be involved in plant defense and development by methylating ja to methyl jasmonate (meja) and thus influencing the concentrations of ja and related metabolites. however, no jmt gene has been well characterized in monocotyledon defense and development at the molecular level. after we cloned a rice jmt gene, osjmt1, whose encoding protein was localized in the cytosol, we ... | 2016 | 26466818 |
| differential interactions of 5-(4-piperidyl)-3-isoxazolol analogues with insect γ-aminobutyric acid receptors leading to functional selectivity. | γ-aminobutyric acid (gaba) receptors (gabars) mediate fast inhibitory synaptic transmission and are also targets for drugs and insecticides. to better understand the molecular interactions of ligands with the orthosteric sites of gabars, we examined 4-aryl/arylalkyl-5-(4-piperidyl)-3-isoxazolol, 4-aryl-5-(4-piperidyl)-3-isothiazolol, and 5-aryl-4-(4-piperidyl)-1-hydroxypyrazole for their antagonism with regard to three insect gabars. the 3-isoxazolol was preferable to the 3-isothiazolol and 1-hy ... | 2015 | 26453818 |
| argonaute subfamily genes in the small brown planthopper, laodelphax striatellus (hemiptera: delphacidae). | argonaute (ago) proteins are essential catalytic components of the rna-induced silencing complex and play central roles in rna interference. using a combination of bioinformatics and rapid amplification of cdna ends (race) methods, putative ago subfamily members, ls-ago1 and ls-ago2, were cloned and characterized from the small brown planthopper, laodelphax striatellus. the open reading frame (orf) of ls-ago1 is 2,820 bp long, encoding a putative protein of 939 amino acid residues, and ls-ago2 c ... | 2016 | 26446351 |
| genome-wide identification of long noncoding rna genes and their potential association with fecundity and virulence in rice brown planthopper, nilaparvata lugens. | the functional repertoire of long noncoding rna (lncrna) has been characterized in several model organisms, demonstrating that lncrna plays important roles in fundamental biological processes. however, they remain largely unidentified in most species. understanding the characteristics and functions of lncrna in insects would be useful for insect resources utilization and sustainable pest control. | 2015 | 26437919 |
| roles of nlaktip in the growth and eclosion of the rice brown planthopper, nilaparvata lugens stål, as revealed by rna interference. | akt-interacting protein (aktip) interacts with serine/threonine protein kinase b (pkb)/akt. aktip modulates akt's activity by enhancing the phosphorylation of the regulatory site and plays a crucial role in multiple biological processes. in this study, the full length cdna of nlaktip, a novel aktip gene in the brown planthopper (bph) nilaparvata lugens, was cloned. the reverse transcription quantitive pcr (rt-qpcr) results showed that the nlaktip gene was strongly expressed in gravid female adul ... | 2015 | 26402675 |
| jinggangmycin increases fecundity of the brown planthopper, nilaparvata lugens (stål) via fatty acid synthase gene expression. | the antibiotic jinggangmycin (jgm) is mainly used in controlling the rice sheath blight, rhizoctonia solani, in china. jgm also enhances reproduction of the brown planthopper (bph), nilaparvata lugens (stål). to date, however, molecular mechanisms of the enhancement are unclear. our related report documented the influence of foliar jgm sprays on ovarian protein content. here, we used isobaric tags for relative and absolute quantitation (itraq) protocols to analyze ovarian proteins of bph females ... | 2016 | 26388431 |
| systematic analysis of rice (oryza sativa) metabolic responses to herbivory. | plants defend against attack from herbivores by direct and indirect defence mechanisms mediated by the accumulation of phytoalexins and release of volatile signals, respectively. while the defensive arsenals of some plants, such as tobacco and arabidopsis are well known, most of rice's (oryza sativa) defence metabolites and their effectiveness against herbivores remain uncharacterized. here, we used a non-biassed metabolomics approach to identify many novel herbivory-regulated metabolic signatur ... | 2016 | 26386366 |
| genomic analysis of an ascomycete fungus from the rice planthopper reveals how it adapts to an endosymbiotic lifestyle. | a number of sap-sucking insects harbor endosymbionts, which are thought to play an important role in the development of their hosts. one of the most important rice pests, the brown planthopper (bph), nilaparvata lugens (stål), harbors an obligatory yeast-like symbiont (yls) that cannot be cultured in vitro. genomic information on this yls would be useful to better understand its evolution. in this study, we performed genome sequencing of the yls using both 454 and illumina approaches, generating ... | 2015 | 26338189 |
| changes in endosymbiotic bacteria of brown planthoppers during the process of adaptation to different resistant rice varieties. | the specific primers of five species of endosymbiotic bacteria were designed to determine their numbers in three virulent populations of brown planthopper, nilapavata lugens stål, and to assess changes during adaptation to different resistant varieties using fluorescent quantitative pcr. the results showed that chryseobacterium was the dominant bacteria in all three populations of brown planthopper, followed by acinetobacter in tn1 population, arsenophonus and serratia in mudgo population, and a ... | 2015 | 26313963 |
| susceptibility of sogatella furcifera and laodelphax striatellus (hemiptera: delphacidae) to six insecticides in china. | the whitebacked planthopper, sogatella furcifera (horváth), and small brown planthopper, laodelphax striatellus (fallén), both are important crop pests throughout china, especially in rice. application of chemical insecticides is the major control practice. consequently, insecticide resistance has become an urgent issue. in this study, resistance levels to six conventional insecticides were evaluated for these two species collected from major occurring areas of china. additionally, imidacloprid- ... | 2014 | 26309282 |
| the β-n-acetylhexosaminidase gene family in the brown planthopper, nilaparvata lugens. | β-n-acetylhexosaminidases (hexs) are enzymes that can degrade the chitin oligosaccharides that are produced by the activity of chitinases on chitin in insects. using bioinformatic methods based on genome and transcriptome databases, 11 β-n-acetylhexosaminidase genes (nlhexs) in nilaparvata lugens were identified and characterized. phylogenetic analysis revealed a six-grouped tree topology. the o-linked n-acetylglucosaminidase (o-glcnacase) group includes nlhex11, which harbours a catalytic domai ... | 2015 | 26304035 |
| reduction in mrna and protein expression of a nicotinic acetylcholine receptor α8 subunit is associated with resistance to imidacloprid in the brown planthopper, nilaparvata lugens. | target-site resistance is commonly caused by qualitative changes in insecticide target-receptors and few studies have implicated quantitative changes in insecticide targets in resistance. here we show that resistance to imidacloprid in a selected strain of nilaparvata lugens is associated with a reduction in expression levels of the nicotinic acetylcholine receptor (nachr) subunit nlα8. synergism bioassays of the selected strain suggested resistance was conferred, in part, by a target-site mecha ... | 2015 | 26259922 |
| genomic and transcriptomic insights into the cytochrome p450 monooxygenase gene repertoire in the rice pest brown planthopper, nilaparvata lugens. | the cytochrome p450 monooxygenase (p450) gene family is one of the most abundant eukaryotic gene families that encode detoxification enzymes. in this study, we identified an abundance of p450 gene repertoire through genome- and transcriptome-wide analysis in the brown planthopper (nilaparvata lugens), the most destructive rice pest in asia. detailed gene information including the exon-intron organization, size, transcription orientation and distribution in the genome revealed that many p450 loci ... | 2015 | 26234643 |
| molecular characterization, tissue and developmental expression profiles of cryptochrome genes in wing dimorphic brown planthoppers, nilaparvata lugens. | cryptochromes (crys) are blue and uv light photoreceptors, known to play key roles in circadian rhythms and in the light-dependent magnetosensitivity of insects. two novel cryptochrome genes were cloned from the brown planthopper, and were given the designations of nlcry1 and nlcry2, with the accession numbers km108578 and km108579 in genbank. the complementary dna sequences of nlcry1 and nlcry2 are 1935 bp and 2463 bp in length, and they contain an open reading frame of 1629 bp and 1872 bp, enc ... | 2016 | 26227859 |
| herbivory-induced glucose transporter gene expression in the brown planthopper, nilaparvata lugens. | nilaparvata lugens, the brown planthopper (bph) feeds on rice phloem sap, containing high amounts of sucrose as a carbon source. nutrients such as sugars in the digestive tract are incorporated into the body cavity via transporters with substrate selectivity. eighteen sugar transporter genes of bph (nlst) were reported and three transporters have been functionally characterized. however, individual characteristics of nlst members associated with sugar transport remain poorly understood. comparat ... | 2015 | 26226652 |
| establishing the role of detoxifying enzymes in field-evolved resistance to various insecticides in the brown planthopper (nilaparvata lugens) in south india. | the brown planthopper (bph), nilaparvata lugens (stål), is one of the major pests of rice throughout asia. extensive use of insecticides for suppressing n. lugens has resulted in the development of insecticide resistance leading to frequent control failures in the field. the aim of the present study was to evaluate resistance in the field populations of n. lugens from major rice growing states of south india to various insecticides. we also determined the activity of detoxifying enzymes (esteras ... | 2017 | 26200805 |
| genetic analysis and molecular mapping of qtls for resistance to rice black-streaked dwarf disease in rice. | rice black-streaked dwarf disease, caused by rice black-streaked dwarf virus (rbsdv), is transmitted by small brown planthoppers (laodelphax striatellus fallén, sbph) and causes severe yield loss in epidemic years in china and other east asian countries. breeding for resistance to rbsdv is a promising strategy to control the disease. we identified tetep that showed resistance to rbsdv using a field test and artificial inoculation test. an evaluation of the resistance mechanism revealed that tete ... | 2015 | 26198760 |
| silencing a sugar transporter gene reduces growth and fecundity in the brown planthopper, nilaparvata lugens (stål) (hemiptera: delphacidae). | the brown planthopper (bph), nilaparvata lugens, sugar transporter gene 6 (nlst6) is a facilitative glucose/fructose transporter (often called a passive carrier) expressed in midgut that mediates sugar transport from the midgut lumen to hemolymph. the influence of down regulating expression of sugar transporter genes on insect growth, development, and fecundity is unknown. nonetheless, it is reasonable to suspect that transporter-mediated uptake of dietary sugar is essential to the biology of ph ... | 2015 | 26185058 |
| whole-genome expression analysis of rice black-streaked dwarf virus in different plant hosts and small brown planthopper. | rice black-streaked dwarf virus (rbsdv) can infect a number of gramineous plants and cause severe crop yield losses in southeast asian countries. the virus is transmitted by small brown planthopper (sbph) in a persistent circulative manner. the interactions between rbsdv and its different hosts remain unknown. besides, how the virus adjusts itself to infect different hosts is unclear. in the present study, the relative rna levels of the thirteen rbsdv genes in rice, maize, wheat, and sbph were m ... | 2015 | 26149652 |
| map-based cloning and characterization of bph29, a b3 domain-containing recessive gene conferring brown planthopper resistance in rice. | rice (oryza sativa l.) production, essential for global food security, is threatened by the brown planthopper (bph). the breeding of host-resistant crops is an economical and environmentally friendly strategy for pest control, but few resistance gene resources have thus far been cloned. an indica rice introgression line rbph54, derived from wild rice oryza rufipogon, has been identified with sustainable resistance to bph, which is governed by recessive alleles at two loci. in this study, a map-b ... | 2015 | 26136269 |
| quantification of southern rice black streaked dwarf virus and rice black streaked dwarf virus in the organs of their vector and nonvector insect over time. | southern rice black streaked dwarf virus (srbsdv) and rice black streaked dwarf virus (rbsdv) are serious rice-infecting reoviruses, which are transmitted by different planthoppers in a persistent propagative manner. in this study, we quantitatively compared the spatial distribution of srbsdv and rbsdv contents over time in their vector and nonvector insects using real time-pcr. genome equivalent copies (gec) were assessed every 2 days from 0 to 14 days after a 3-days acquisition access period ( ... | 2015 | 26116274 |
| the multicopper oxidase gene family in the brown planthopper, nilaparvata lugens. | the multicopper oxidase (mco) family of enzymes includes laccases, ascorbate oxidases, bilirubin oxidases and a subgroup of metal oxidases. on the basis of a bioinformatics investigation, we identified 7 genes encoding putative multicopper oxidase proteins in the genome of the brown planthopper (bph), nilaparvata lugens (hemiptera: delphacidae). mco1 and mco2 are conserved, while others diverse in insects. analysis of developmental and tissue-specific expression patterns revealed the following: ... | 2015 | 26107750 |
| proteomic analysis of interaction between a plant virus and its vector insect reveals new functions of hemipteran cuticular protein. | numerous viruses can be transmitted by their corresponding vector insects; however, the molecular mechanisms enabling virus transmission by vector insects have been poorly understood, especially the identity of vector components interacting with the virus. here, we used the yeast two-hybrid system to study proteomic interactions of a plant virus (rice stripe virus, rsv, genus tenuivirus) with its vector insect, small brown planthopper (laodelphax striatellus). sixty-six proteins of l. striatellu ... | 2015 | 26091699 |
| bioefficacy of piper ribesioides (piperaceae) extracts against nilaparvata lugens stal. (homoptera: delphacidae). | the extracts of stem and seeds of piper ribesioides (piperaceae) were prepared in hexane, dichloromethane, ethyl acetate and ethanol and evaluated for their efficacy against 3-day-old adult brown plant hoppers, nilaparvata lugens stal under laboratory conditions. the treatment was given topically and results suggest the dose dependent response related to exposure time. the ethyl acetate extract was the best with an lc50 = 738 ppm-, 24 h post-treatment. there was substantial increase in activity ... | 2014 | 26084101 |
| prioritizing plant defence over growth through wrky regulation facilitates infestation by non-target herbivores. | plants generally respond to herbivore attack by increasing resistance and decreasing growth. this prioritization is achieved through the regulation of phytohormonal signaling networks. however, it remains unknown how this prioritization affects resistance against non-target herbivores. in this study, we identify wrky70 as a specific herbivore-induced, mitogen-activated protein kinase-regulated rice transcription factor that physically interacts with w-box motives and prioritizes defence over gro ... | 2015 | 26083713 |
| rice ragged stunt virus-induced apoptosis affects virus transmission from its insect vector, the brown planthopper to the rice plant. | most plant viruses that seriously damage agricultural crops are transmitted by insects. however, the mechanisms enabling virus transmission by insect vectors are poorly understood. the brown planthopper (nilaparvata lugens) is one of the most serious rice pests, causing extensive damage to rice plants by sucking the phloem sap and transmitting viruses, including rice ragged stunt virus (rrsv). in this study, we investigated the mechanisms of rrsv transmission from its insect vector to the rice p ... | 2015 | 26073458 |
| phloem-exudate proteome analysis of response to insect brown plant-hopper in rice. | brown plant-hopper (nilaparvata lugens stål, bph), one of the most devastating agricultural insect pests of rice throughout asia, ingests nutrients from rice sieve tubes and causes a dramatic yield loss. planting resistant variety is an efficient and economical way to control this pest. understanding the mechanisms of host resistance is extremely valuable for molecular design of resistant rice variety. here, we used an itraq-based quantitative proteomics approach to perform analysis of protein e ... | 2015 | 26072143 |
| identification and function analysis of enolase gene nleno1 from nilaparvata lugens (stål) (hemiptera:delphacidae). | the enolase [ec 4.2.1.11] is an essential enzyme in the glycolytic pathway catalyzing the conversion of 2-phosphoglycerate (2-pge) to phosphoenolpyruvate (pep). in this study, a full-length cdna encoding α-enolase was cloned from rice brown planthopper (nilaparvata lugens) and is provisionally designated as nleno1. the cdna sequence of nleno1 was 1,851 bp with an open reading frame (orf) of 1,305 bp and encoding 434 amino acids. the deduced protein shares high identity of 80-87% with eno1-like p ... | 2015 | 26056319 |
| dna methyltransferases have an essential role in female fecundity in brown planthopper, nilaparvata lugens. | dna methylation is an ancient epigenetic modification present in all three domains of life. however, the understanding of dna methylation in insects is limited. here, we amplified the full-length transcripts of the dna methyltransferases nlu-dnmt1 and nlu-dnmt3, indicating that a complete dna methylation toolkit exists in the brown planthopper, nilaparvata lugens, a destructive pest in rice production. nlu-dnmt1 and nlu-dnmt3 had the conserved motifs and domains of the dna methyltransferase fami ... | 2015 | 26043687 |
| bicaudal-c plays a vital role in oogenesis in nilaparvata lugens (hemiptera: delphacidae). | bicaudal-c (bic-c) was originally identified in a drosophila melanogaster mutagenesis screen and plays vital roles in embryogenesis. in this study, we characterized the bic-c gene in the brown planthopper, nilaparvata lugens (hemiptera: delphacidae), an insect pest that undergoes incomplete metamorphosis. our result showed that n. lugens bic-c (nlbic-c) is a female-specific gene in this species. it is specifically expressed in developing oocytes and is not expressed in laid eggs. ribonucleic aci ... | 2015 | 26025198 |
| activation of the tor signalling pathway by glutamine regulates insect fecundity. | the target of rapamycin (tor) positively controls cell growth in response to nutrients such as amino acids. however, research on the specific nutrients sensed by tor is limited. glutamine (gln), a particularly important amino acid involved in metabolism in organisms, is synthesised and catalysed exclusively by glutamine synthetase (gs), and our previous studies have shown that gln may regulate fecundity in vivo levels of the brown planthopper (bph) nilaparvata lugens. until now, it has remained ... | 2015 | 26024507 |
| pathways of amino acid degradation in nilaparvata lugens (stål) with special reference to lysine-ketoglutarate reductase/saccharopine dehydrogenase (lkr/sdh). | nilaparvata lugens harbors yeast-like symbionts (ylss). in present paper, a genome-wide analysis found 115 genes from ni. lugens and 90 genes from ylss that were involved in the metabolic degradation of 20 proteinogenic amino acids. these 205 genes encoded for 77 enzymes. accordingly, the degradation pathways for the 20 amino acids were manually constructed. it is postulated that ni. lugens can independently degrade fourteen amino acids (threonine, alanine, glycine, serine, aspartate, asparagine ... | 2015 | 26000452 |
| apterous a modulates wing size, bristle formation and patterning in nilaparvata lugens. | apterous a (apa), a member of the lim-homeobox gene family, plays a critical role in the development of wing. the achaete-scute complex (as-c) encodes basic helix-loop-helix (bhlh) transcription factors and functions in bristle development. in the present study, we cloned apa (nlapa) and an achaete-scute homologue (nlash) from n. lugens. levels of nlapa and nlash were higher in nymphs than adults, with particularly high expression in the thorax of nymphs. nlapa expressed more highly in nymphs of ... | 2015 | 25995006 |
| rna interference-aided knockdown of a putative saccharopine dehydrogenase leads to abnormal ecdysis in the brown planthopper, nilaparvata lugens (stål) (hemiptera: delphacidae). | the brown planthopper nilaparvata lugens is a serious phloem-feeding pest of rice in china. the current study focuses on a saccharopine dehydrogenase (sdh) that catalyzes the penultimate reaction in biosynthesis of the amino acid lysine (lys), which plays a role in insect growth and carnitine production (as a substrate). the protein, provisionally designated as nlylssdh [a sdh derived from yeast-like symbiont (yls) in n. lugens], had a higher transcript level in abdomens, compared with heads, wi ... | 2015 | 25908053 |
| [stress effects of imidacloprid on rsv in rice plants]. | the rice stripe disease is a viral disease transmitted by small brown planthopper, laodelphax striatellus, which outbroke a few years ago in the yangtze river basin, especially jiangsu region, china. to study the effects of imidacloprid stress on rice stripe virus (rsv) in rice plants, the rice seedlings were treated with imidacloprid 1, 2, 3 and 4 times (b1, b2, b3 and b4), respectively, after artificial inoculation by l. striatellus for 48 h, and the expression levels of relative genes includi ... | 2014 | 25876413 |
| molecular cloning and expression profile of an atp-binding cassette (abc) transporter gene from the hemipteran insect nilaparvata lugens. | the atp-binding cassette (abc) transporters belong to a large superfamily of proteins that have important physiological functions in all living organisms. in insects, abc transporters have important functions in the transport of molecules, and are also involved in insecticide resistance, metabolism, and development. in this study, the nilaparvata lugens stal (hemiptera: delphacidae) abcg (nlabcg) gene was identified and characterized. the complete mrna sequence of nlabcg was 2608-bp long, with a ... | 2015 | 25867414 |
| pseudo-backcrossing design for rapidly pyramiding multiple traits into a preferential rice variety. | pyramiding multiple genes into a desirable genetic background can take years to accomplish. in this paper, a pseudo-backcrossing scheme was designed to shorten the backcrossing cycle needed. pink3, an aromatic and potentially high-yielding rice variety-although one that is intolerant to flash flooding (sub) and susceptible to bacterial leaf blight (bb), leaf-neck blast (bl) and the brown planthopper (bph)-was used as a genetic basis for significant improvements through gene pyramiding. | 2015 | 25844112 |
| knockdown of the ionotropic γ-aminobutyric acid receptor (gabar) rdl gene decreases fipronil susceptibility of the small brown planthopper, laodelphax striatellus (hemiptera: delphacidae). | insect γ-aminobutyric acid receptors (gabars) are important molecular targets of cyclodiene and phenylpyrazole insecticides. previously gabars encoding rdl (resistant to dieldrin) genes responsible for dieldrin and fipronil resistance were identified in various economically important insect pests. in this study, we cloned the open reading frame cdna sequence of rdl gene from fipronil-susceptible and fipronil-resistant strains of laodelphax striatellus (lsrdl). sequence analysis confirmed the pre ... | 2015 | 25808850 |
| facilitation of rice stripe virus accumulation in the insect vector by himetobi p virus vp1. | the small brown planthopper (sbph) is the main vector for rice stripe virus (rsv), which causes serious rice stripe disease in east asia. to characterize the virus-vector interactions, the sbph cdna library was screened with rsv ribonucleoprotein (rnp) as bait using a gal4-based yeast two-hybrid system. the interaction between rsv-rnp and the himetobi p virus (hipv, an insect picorna-like virus) vp1 protein was identified. the relationships between hipv and rsv in sbph were further investigated, ... | 2015 | 25807055 |
| two insulin receptors determine alternative wing morphs in planthoppers. | wing polyphenism is an evolutionarily successful feature found in a wide range of insects. long-winged morphs can fly, which allows them to escape adverse habitats and track changing resources, whereas short-winged morphs are flightless, but usually possess higher fecundity than the winged morphs. studies on aphids, crickets and planthoppers have revealed that alternative wing morphs develop in response to various environmental cues, and that the response to these cues may be mediated by develop ... | 2015 | 25799997 |
| [ecological responses of brown planthopper, nilaparvata lugens, to triazophos and deltamethrin on transgenic bt rice lines and their non-bt parental rice]. | in this study, the brown planthopper (bph), nilaparvata lugens (hemiptera: delphacidae), cultured on bt rice lines (t1c-19 with cry1c gene and t2a-1 with cry2a gene) and their parental rice mh63, were treated with a series of concentrations of triazophos and deltamethrin when they developed up to the third instar for the evaluation of the effects of bt rice on bph resurgence induced by pesticides. the results indicated that bph nymphal duration decreased with the increasing concentration of delt ... | 2014 | 25796912 |
| take-off time of the first generation of the overwintering small brown planthopper, laodelphax striatellus in the temperate zone in east asia. | overseas migration of the small brown planthopper, laodelphax striatellus (fallén), occurs during the winter wheat harvest season in east asia. knowing the take-off time of emigrating l. striatellus is crucial for predicting such migrations with a simulation technique because winds, carriers of migratory insects, change continuously. several methods were used in china and japan from late may to early june 2012 and again in 2013 to identify the precise timing of take-off. these methods included: ... | 2015 | 25780936 |
| the insecticidal activity and action mode of an imidacloprid analogue, 1-(3-pyridylmethyl)-2-nitroimino-imidazolidine. | neonicotinoids, such as imidacloprid, are key insecticides extensively used for control of nilaparvata lugens. however, imidacloprid resistance has been reported in many asian countries in recent years. to understand the roles of the chlorine atom of pyridyl group on insecticidal activity and resistance, the atom was removed to generate an imidacloprid analogue dc-imi (deschlorine imidacloprid). dc-imi showed significantly higher toxicity than imidacloprid in the susceptible strain of n. lugens, ... | 2016 | 25755197 |
| the effects of triazophos applied to transgenic bt rice on the nutritional indexes, nlvg expression, and population growth of nilaparvata lugens stål under elevated co₂. | the brown planthopper, nilaparvata lugens (stål) (hemiptera: delphacidae), is a typical pest in which population resurgence can be induced by insecticides. warmer global temperatures, associated with anthropogenic climate change, are likely to have marked ecological effects on terrestrial ecosystems. however, the effects of elevated co2 (eco2) concentrations on the resurgence of n. lugens that have been treated with pesticides used for transgenic bt rice cultivation are not fully understood. the ... | 2015 | 25752430 |
| study of the long-distance migration of small brown planthoppers laodelphax striatellus in china using next-generation sequencing. | the small brown planthopper (laodelphax striatellus) is a widespread insect pest of rice in east asia. previous studies have shown the long-distance migrations undertaken by l. striatellus, but have not provided molecular evidence to support this. | 2016 | 25684265 |
| a new finely mapped oryza australiensis-derived qtl in rice confers resistance to brown planthopper. | brown planthopper (bph) is the most destructive pest of rice in asia. the bph resistance in the introgression line ir65482-17-511-5-7 (ir65482-17) is derived from the wild rice species oryza australiensis. an f2:3 population from a cross between zhenshan 97 (zs97) and ir65482-17 was used to map three quantitative trait loci (qtls) for seedling resistance and feeding rate to bph. the loci were distributed on chromosomes 2, 4 and 12. the qtl qbph4.2 on chromosome 4 had the largest effect, and cont ... | 2015 | 25682936 |
| rice stripe tenuivirus nonstructural protein 3 hijacks the 26s proteasome of the small brown planthopper via direct interaction with regulatory particle non-atpase subunit 3. | the ubiquitin/26s proteasome system plays a vital role in regulating host defenses against pathogens. previous studies have highlighted different roles for the ubiquitin/26s proteasome in defense during virus infection in both mammals and plants, but their role in the vectors that transmit those viruses is still unclear. in this study, we determined that the 26s proteasome is present in the small brown planthopper (sbph) (laodelphax striatellus) and has components similar to those in plants and ... | 2015 | 25653432 |
| a floatable formulation and laboratory bioassay of pandora delphacis (entomophthoromycota: entomophthorales) for the control of rice pest nilaparvata lugens stål (hemiptera: delphacidae). | the brown planthopper (bph), nilaparvata lugens (staparvata luera: delphacidae), is a serious rice pest that easily develops resistance to chemical insecticides and resistant rice varieties. this study evaluated the infectivity of the bph fungal pathogen, pandora delphacis, and developed a novel formulation as an alternative means of bph control. | 2016 | 25641904 |
| transgenic plants expressing the aait/gna fusion protein show increased resistance and toxicity to both chewing and sucking pests. | the adoption of pest-resistant transgenic plants to reduce yield losses and decrease pesticide use has been successful. to achieve the goal of controlling both chewing and sucking pests in a given transgenic plant, we generated transgenic tobacco, arabidopsis, and rice plants expressing the fusion protein, aait/gna, in which an insecticidal scorpion venom neurotoxin (androctonus australis toxin, aait) is fused to snowdrop lectin (galanthus nivalis agglutinin, gna). compared with transgenic tobac ... | 2016 | 25641865 |
| evidence for high dispersal ability and mito-nuclear discordance in the small brown planthopper, laodelphax striatellus. | understanding dispersal ability in pest species is critical for both theoretical aspects of evolutionary and population biology and from a practical standpoint, such as implementing effective forecasting systems. the small brown planthopper (sbph), laodelphax striatellus (fallén), is an economically important pest, but few data exist on its dispersal ability. here, we used mitochondrial and nuclear markers to elucidate the population genetic structure of sbph and of the parasitic bacterium wolba ... | 2015 | 25622966 |
| identification of polymorphisms in cyrtorhinus lividipennis rdl subunit contributing to fipronil sensitivity. | as one of the most important predatory enemies, the miridbug, cyrtorhinus lividipennis, plays an important role in rice planthoppers control, such as nilaparvata lugens (brown planthopper). in order to compare insecticide selectivity between c. lividipennis and n. lugens, the contact acute toxicities of six insecticides (diazoxon, paraoxon, carbaryl, fenobucarb, fipronil and ethofenprox) were monitored. the results showed that all tested insecticides were more toxic to c. lividipennis than to n. ... | 2015 | 25619913 |
| molecular characterization and rna interference analysis of vitellogenin receptor from nilaparvata lugens (stål). | vitellogenin receptors (vgrs), members of the low-density lipoprotein receptor (ldlr) superfamily, are responsible for taking vitellogenin (vg) into developing oocytes. here the first full-length vgr cdna from a hemipteran insect, the brown planthopper (nilaparvata lugens), was cloned and sequenced. the complete mrna sequence was 6174 bp in length with an open reading frame (orf) of 5796 bp encoding 1931 amino acid residues. n. lugens vgr (nlvgr) contained two ligand-binding domains with five ld ... | 2015 | 25617689 |
| identification of key amino acid differences between cyrtorhinus lividipennis and nilaparvata lugens nachr α8 subunits contributing to neonicotinoid sensitivity. | high sensitivity to neonicotinoid insecticides have been reported in the miridbug cyrtorhinus lividipennis, an important predatory enemy of rice planthoppers, such as nilaparvata lugens (brown planthopper). in the present study, the sensitivity of neonicotinoid insecticides between c. lividipennis and n. lugens were detected and compared. the results showed that neonicotinoid insecticides were much more toxic to the miridbug than to the brown planthopper. a nicotinic acetylcholine receptor subun ... | 2015 | 25613467 |
| field-evolved resistance to imidacloprid and ethiprole in populations of brown planthopper nilaparvata lugens collected from across south and east asia. | we report on the status of imidacloprid and ethiprole resistance in nilaparvata lugens stål collected from across south and east asia over the period 2005-2012. | 2016 | 25612154 |
| tetrahydroindeno[1',2':4,5]pyrrolo[1,2-a]imidazol-5(1h)-ones as novel neonicotinoid insecticides: reaction selectivity and substituent effects on the activity level. | tetraheterocyclic tetrahydroindeno[1',2':4,5]pyrrolo[1,2-a]imidazol-5(1h)-one derivatives as novel neonicotinoid candidates were designed and prepared by selective etherification, chlorination and esterification of ninhydrin-heterocyclic ketene aminals adducts. some of the designed compounds showed excellent insecticidal activity against cowpea aphids (aphis craccivora), brown planthopper (nilaparvata lugens), and armyworm (mythimna separata). in particular, the activity against armyworm (mythim ... | 2015 | 25611859 |
| differentially regulated genes in the salivary glands of brown planthopper after feeding in resistant versus susceptible rice varieties. | brown planthopper (bph) is a damaging insect pest of rice. we used suppression subtractive hybridization (ssh) and mirror orientation selection to identify differentially regulated genes in salivary glands of bph after feeding on resistant and susceptible varieties. the forward ssh library included 768 clones with insertions ranging from 250 to 1000 bp. after differential screening, a total of 112 transcripts were identified, which included 27 upregulated genes and seven downregulated genes. sev ... | 2015 | 25611813 |
| genomes of the rice pest brown planthopper and its endosymbionts reveal complex complementary contributions for host adaptation. | the brown planthopper, nilaparvata lugens, the most destructive pest of rice, is a typical monophagous herbivore that feeds exclusively on rice sap, which migrates over long distances. outbreaks of it have re-occurred approximately every three years in asia. it has also been used as a model system for ecological studies and for developing effective pest management. to better understand how a monophagous sap-sucking arthropod herbivore has adapted to its exclusive host selection and to provide in ... | 2014 | 25609551 |
| rice stripe virus affects the viability of its vector offspring by changing developmental gene expression in embryos. | plant viruses may affect the viability and development process of their herbivore vectors. small brown planthopper (sbph) is main vector of rice stripe virus (rsv), which causes serious rice stripe disease. here, we reported the effects of rsv on sbph offspring by crossing experiments between viruliferous and non-viruliferous strains. the life parameters of offspring from different cross combinations were compared. the hatchability of f1 progeny from viruliferous parents decreased significantly, ... | 2015 | 25601039 |
| the genetic basis of population fecundity prediction across multiple field populations of nilaparvata lugens. | identifying the molecular markers for complex quantitative traits in natural populations promises to provide novel insight into genetic mechanisms of adaptation and to aid in forecasting population dynamics. in this study, we investigated single nucleotide polymorphisms (snps) using candidate gene approach from high- and low-fecundity populations of the brown planthopper (bph) nilaparvata lugens stål (hemiptera: delphacidae) divergently selected for fecundity. we also tested whether the populati ... | 2015 | 25581109 |
| the genome of the nucleopolyhedrosis-causing virus from tipula oleracea sheds new light on the nudiviridae family. | a large double-stranded dna (dsdna) virus that produces occlusion bodies, typical of baculoviruses, has been described to infect crane fly larvae of the genus tipula (diptera, tipulidae). because of a lack of genomic data, this virus has remained unclassified. electron microscopy of an archival virus isolated from tipula oleracea, t. oleracea nudivirus (tonv), showed irregularly shaped occlusion bodies measuring from 2 to 5 μm in length and 2 μm in middiameter, filled with rod-shape virions cont ... | 2014 | 25540386 |
| different expression profiles suggest functional differentiation among chemosensory proteins in nilaparvata lugens (hemiptera: delphacidae). | chemosensory proteins (csps) play various roles in insect physiology including olfaction and development. the brown planthopper, nilaparvata lugens stål, is one of the most notorious rice pests worldwide. the wing-from variation and annually long distance migration imply that olfaction would play a key role in n. lugens behavior. in this study, full-length cdnas of nine csps were cloned by the rapid amplification of cdna ends procedure, and their expression profiles were determined by the quanti ... | 2014 | 25527582 |
| the insect ecdysone receptor is a good potential target for rnai-based pest control. | rna interference (rnai) has great potential for use in insect pest control. however, some significant challenges must be overcome before rnai-based pest control can become a reality. one challenge is the proper selection of a good target gene for rnai. here, we report that the insect ecdysone receptor (ecr) is a good potential target for rnai-based pest control in the brown planthopper nilaparvata lugens, a serious insect pest of rice plants. we demonstrated that the use of a 360 bp fragment (nl ... | 2014 | 25516715 |
| effects of exogenous plant growth regulator abscisic acid-induced resistance in rice on the expression of vitellogenin mrna in nilaparvata lugens (hemiptera: delphacidae) adult females. | recent study showed that exogenous abscisic acid (aba) acts as a regulator of plant resistance. this study investigated average injury scale and callose contents of rice, and vitellogenin (nlvg) mrna expression in nilaparvata lugens (stål) (hemiptera: delphacidae) adult females after third instar nymphs fed on exogenous aba-treated susceptible [taichung native one (tn1)] and moderately resistant (ir42) rice cultivars. the results showed that exogenous aba significantly decreased average injury s ... | 2014 | 25502025 |
| pichia anomala, a new species of yeast-like endosymbionts and its variation in small brown planthopper (laodelphax striatellus). | yeast-like symbionts (yls) are endosymbionts that promote the growth of delphacid planthoppers (hemiptera: delphacidae), some of which are pests on cultivated rice. identification and characterization of yls growth can be helpful for pest control, because it has been demonstrated that there is a variety of yls in rice planthopper and they affected the planthopper's growth and virulence to plant hosts. so, elucidation of the species of yls in planthopper is crucial for exploiting a new way to con ... | 2015 | 25499750 |
| structural characterization of the catalytic site of a nilaparvata lugens delta-class glutathione transferase. | glutathione transferases (gsts) are a major class of detoxification enzymes that play a central role in the defense against environmental toxicants and oxidative stress. here, we studied the crystal structure of a delta-class glutathione transferase from nilaparvata lugens, nlgstd, to gain insights into its catalytic mechanism. the structure of nlgstd in complex with glutathione, determined at a resolution of 1.7å, revealed that it exists as a dimer and its secondary and tertiary structures are ... | 2015 | 25497345 |
| a simplified method for simultaneous detection of rice stripe virus and rice black-streaked dwarf virus in insect vector. | rice stripe virus (rsv) and rice black-streaked dwarf virus (rbsdv) are transmitted by their common vector small brown planthopper (sbph) that cause serious crop losses in china. a simple reverse transcription-pcr method was developed for the simultaneous detection of rsv and rbsdv in single sbph. three primers targeted to rsv-rna4 and rbsdv-s2 segments were designed to amplify respectively 1114-bp and 414-bp fragments in a reaction. the method is reliable, rapid and inexpensive for detecting th ... | 2015 | 25455902 |
| bt rice expressing cry2aa does not harm cyrtorhinus lividipennis, a main predator of the nontarget herbivore nilapavarta lugens. | t2a-1 is a newly developed transgenic rice that expresses a synthesized cry2aa gene driven by the maize ubiquitin promoter. t2a-1 exhibits high resistance against lepidopteran pests of rice. the brown planthopper, nilapavarta lugens (stål), is a main nontarget sap-sucking insect pest of rice, and cyrtorhinus lividipennis (reuter) is the major predator of the eggs and young nymphs of planthoppers. as c. lividipennis may expose to the cry2aa protein via n. lugens, it is therefore essential to asse ... | 2014 | 25375147 |
| selection of beauveria isolates pathogenic to adults of nilaparvata lugens. | the brown planthopper, nilaparvata lugens stål (hemiptera: delphacidae), is a destructive invasive pest and has become one of the most economically-important rice pests in china. effective control measures are desperately needed. entomopathogenic fungi, such as beauveria bassiana (balsamo-crivelli) vuillemin (hypocreales: clavicipitaceae) and b. brongniartii (saccardo), have shown great potential for the management of some sucking pest species. in this study, to explore alternative strategies fo ... | 2014 | 25373179 |
| seven-membered azabridged neonicotinoids: synthesis, crystal structure, insecticidal assay, and molecular docking studies. | to study the influence of the ring sizes, 37 novel seven-membered azabridged neonicotinoid analogues were synthesized by reactions of nitromethylene analogues, succinaldehyde, and aniline hydrochlorides. most of the title compounds presented higher insecticidal activities than that of imidacloprid (imi), cycloxaprid (cyc), and eight-membered compounds against cowpea aphid (aphis craccivora), armyworm (pseudaletia separata walker), and brown planthopper (nilaparvata lugens), which indicated that ... | 2014 | 25347284 |
| effects of two pesticides, tzp and jgm, on reproduction of three planthopper species, nilaparvata lugens stål, sogatella furcifera horvath, and laodelphax striatella fallén. | three planthopper species, the brown planthopper (bph), nilaparvata lugens stål, the white-backed planthopper (wbph), sogatella furcifera horvath, and the small brown planthopper (sbph), laodelphax striatella fallén, often co-occur in rice grown regions of china. the present investigation examined effects of two pesticides, triazophos (tzp) and jinggangmycin (jgm) (a fungicide), on reproduction of bph, wbph, and sbph. the results showed that tzp stimulated the fecundity of the three planthopper ... | 2014 | 25307466 |
| transcriptome analysis of the small brown planthopper, laodelphax striatellus carrying rice stripe virus. | rice stripe virus (rsv), the type member of the genus tenuivirus, transmits by the feeding behavior of small brown planthopper (sbph), laodelphax striatellus. to investigate the interactions between the virus and vector insect, total rna was extracted from rsv-viruliferous sbph (rvls) and non-viruliferous sbph (nvls) adults to construct expressed sequence tag databases for comparative transcriptome analysis. over 30 million bases were sequenced by 454 pyrosequencing to construct 1,538 and 953 of ... | 0 | 25288960 |
| estimation of the age and amount of brown rice plant hoppers based on bionic electronic nose use. | the brown rice plant hopper (brph), nilaparvata lugens (stal), is one of the most important insect pests affecting rice and causes serious damage to the yield and quality of rice plants in asia. this study used bionic electronic nose technology to sample brph volatiles, which vary in age and amount. principal component analysis (pca), linear discrimination analysis (lda), probabilistic neural network (pnn), bp neural network (bpnn) and loading analysis (loadings) techniques were used to analyze ... | 2014 | 25268913 |
| wingless gene cloning and its role in manipulating the wing dimorphism in the white-backed planthopper, sogatella furcifera. | wingless gene (wg) plays a fundamental role in regulating the segment polarity and wing imaginal discs of insects. the rice planthoppers have an obvious wing dimorphism, and the long- and short-winged forms exist normally in natural populations. however, the molecular characteristics and functions of wg in rice planthoppers are poorly understood, and the relationship between expression level of wg and wing dimorphism has not been clarified. | 2014 | 25266639 |
| selection of nectar plants for use in ecological engineering to promote biological control of rice pests by the predatory bug, cyrtorhinus lividipennis, (heteroptera: miridae). | ecological engineering for pest management involves the identification of optimal forms of botanical diversity to incorporate into a farming system to suppress pests, by promoting their natural enemies. whilst this approach has been extensively researched in many temperate crop systems, much less has been done for rice. this paper reports the influence of various plant species on the performance of a key natural enemy of rice planthopper pests, the predatory mirid bug, cyrtorhinus lividipennis. ... | 2014 | 25254377 |
| cis-12-oxo-phytodienoic acid stimulates rice defense response to a piercing-sucking insect. | the brown planthopper (bph, nilaparvata lugens) is a destructive, monophagous, piercing-sucking insect pest of rice. previous studies indicated that jasmonic acid (ja) positively regulates rice defense against chewing insect pests but negatively regulates it against the piercing-sucking insect of bph. we here demonstrated that overexpression of allene oxide cyclase (aoc) but not opr3 (cis-12-oxo-phytodienoic acid (opda) reductase 3, an enzyme adjacent to aoc in the ja synthetic pathway) signific ... | 2014 | 25239066 |
| radar observations of the seasonal migration of brown planthopper (nilaparvata lugens stål) in southern china. | the summer and autumn migrations of the brown planthopper (nilaparvata lugens) were observed in southern china with a millimetric scanning entomological radar and a searchlight trap supplemented with capture in field cages, field surveys, and dissections of females. nilaparvata lugens took off at dusk and dawn in summer, but in autumn there was sometimes only a dusk take-off. the variation of the area density of the radar targets indicated that flight durations were about 9-10 h. in summer, plan ... | 2014 | 25229712 |
| construction of an immobilised acetylcholinesterase column and its application in screening insecticidal constituents from magnolia officinalis. | application of a matrix-immobilised target enzyme for screening inhibitors is widely used in drug development, but there are few studies in insecticide discovery. in this paper, an economical and effective immobilised acetylcholinesterase (ache) column was prepared using the sol-gel embedment method, which was further combined with high-performance liquid chromatography for screening the ache inhibitors and insecticidal compounds from complex natural products. | 2015 | 25228142 |
| chitinase-like gene family in the brown planthopper, nilaparvata lugens. | chitinases are important enzymes required for chitin degradation and reconstruction in insects. based on a bioinformatics investigation, we identified 12 genes encoding putative chitinase-like proteins, including 10 chitinases (cht), one imaginal disc growth factor (idgf) and one endo-β-n-acetylglucosaminidase (engase) in the genome of the brown planthopper, nilaparvata lugens (hemiptera: delphacidae). these 12 genes were clustered into nine different groups, with 11 in glycoside hydrolase famil ... | 2015 | 25224926 |
| infection of rice plants by rice black streaked dwarf virus improves an egg parasitoid, anagrus nilaparvatae (hymenoptera: mymaridae), of rice planthoppers. | the effects of rice plants infected by rice black streaked dwarf virus (rbsdv) on the host preference, duration of immature stages, sex ratio, and adult longevity and parasitic capacity of an egg parasitoid, anagrus nilaparvatae pang et wang, of rice brown planthopper, nilaparvata lugens stål, were evaluated. tests of response to plant volatiles using an olfactometer showed that a. nilaparvatae preferred rice plants harboring rice brown planthopper eggs over plants free of rice brown planthopper ... | 2014 | 25199055 |
| decrease of insecticide resistance over generations without exposure to insecticides in nilaparvata lugens (hemipteran: delphacidae). | the brown planthopper, nilaparvata lugens (stål), is one of the most important insect pests on paddy rice in tropical and temperate asia. overuse and misuse of insecticides have resulted in the development of high resistance to many different insecticides in this pest. studies were conducted to evaluate the change of resistance level to four insecticides over 15 generations without any exposure to insecticides in brown planthopper. after 15 generations' rearing without exposure to insecticide, b ... | 2014 | 25195455 |
| diapause induction and termination in the small brown planthopper, laodelphax striatellus (hemiptera: delphacidae). | the small brown planthopper, laodelphax striatellus (fallén) enters the photoperiodic induction of diapause as 3rd or 4th instar nymphs. the photoperiodic response curves in this planthopper showed a typical long-day response type with a critical daylength of approximately 11 h at 25 °c, 12 h at 22 and 20 °c and 12.5 h at 18 °c, and diapause induction was almost abrogated at 28 °c. the third stage was the most sensitive stage to photoperiod. the photoperiodic response curve at 20 °c showed a gra ... | 2014 | 25188306 |
| comparisons of topical and spray applications of two pesticides, triazophos and jinggangmycin, on the protein content in the ovaries and fat bodies of the brown planthopper nilaparvata lugens stål (hemiptera:delphacidae). | the pesticide-induced stimulation of reproduction in pests is one of the most important mechanisms of pest resurgence. there have been numerous reports on the insecticide-induced stimulation of reproduction. however, the relationship between pesticide application method and pest resurgence (stimulation of reproduction) has received little attention. here, we studied the effect of two treatment methods, triazophos (tzp) and jinggangmycin (jgm), on the protein content in the ovaries and fat bodies ... | 2014 | 25175656 |
| isolation, characterization, and cross-transferability of microsatellite markers from the whitebacked planthopper (sogatella furcifera). | the whitebacked planthopper sogatella furcifera (horváth) (hemiptera: delphacidae) is one of the most harmful pests of rice. in this study, 18 polymorphic microsatellite markers were developed from s. furcifera genomic libraries using the fast isolation by amplified fragment length polymorphism of sequences containing repeat protocols. microsatellite polymorphism was investigated using 32 individuals from one natural population. these 18 simple sequence repeat markers showed a number of alleles ... | 2014 | 25158251 |
| virus-mediated chemical changes in rice plants impact the relationship between non-vector planthopper nilaparvata lugens stål and its egg parasitoid anagrus nilaparvatae pang et wang. | in order to clarify the impacts of southern rice black-streaked dwarf virus (srbsdv) infection on rice plants, rice planthoppers and natural enemies, differences in nutrients and volatile secondary metabolites between infected and healthy rice plants were examined. furthermore, the impacts of virus-mediated changes in plants on the population growth of non-vector brown planthopper (bph), nilaparvata lugens, and the selectivity and parasitic capability of planthopper egg parasitoid anagrus nilapa ... | 2014 | 25141278 |
| identification of promoter polymorphisms in the cytochrome p450 cyp6ay1 linked with insecticide resistance in the brown planthopper, nilaparvata lugens. | imidacloprid resistance in the brown planthopper, nilaparvata lugens, is primarily the result of the over-expression of cytochrome p450 monooxygenases. here, a field-collected strain of n. lugens was shown to be highly resistant to both imidacloprid and buprofezin. insecticide exposure and quantitative real-time pcr revealed that its resistance was mainly associated with a cytochrome p450 gene, cyp6ay1. cyp6ay1 is known to metabolize imidacloprid but its effect on buprofezin is unclear. in the 5 ... | 2014 | 25124988 |
| bac and rna sequencing reveal the brown planthopper resistance gene bph15 in a recombination cold spot that mediates a unique defense mechanism. | brown planthopper (bph, nilaparvata lugens stål), is the most destructive phloem-feeding insect pest of rice (oryza sativa). the bph-resistance gene bph15 has been proved to be effective in controlling the pest and widely applied in rice breeding programs. nevertheless, molecular mechanism of the resistance remain unclear. in this study, we narrowed down the position of bph15 on chromosome 4 and investigated the transcriptome of bph15 rice after bph attacked. | 2014 | 25109872 |
| map-based cloning and characterization of a brown planthopper resistance gene bph26 from oryza sativa l. ssp. indica cultivar adr52. | the brown planthopper (bph) is the most serious insect pest of rice in asia. the indica rice cultivar adr52 carries two bph resistance genes, bph26 (brown planthopper resistance 26) and bph25. map-based cloning of bph26 revealed that bph26 encodes a coiled-coil-nucleotide-binding-site-leucine-rich repeat (cc-nbs-lrr) protein. bph26 mediated sucking inhibition in the phloem sieve element. bph26 was identical to bph2 on the basis of dna sequence analysis and feeding ability of the bph2-virulent bi ... | 2014 | 25076167 |
| a novel, in vivo, indoor method to preserve rice black-streaked dwarf virus in small brown planthopper using wheat seedling as a bridge host. | rice black-streaked dwarf virus (rbsdv) naturally infects gramineae plants through small brown planthopper (sbph) as a vector. however, rbsdv cannot be transmitted to the sbph offspring through transovarian transmission. wheat plant, an important intermediate host in winter, is essential for the completion of the annual cycle of rbsdv in farm ecosystem. we developed a novel, in vivo, indoor method to preserve rbsdv in sbph using wheat seedling as a bridge host. the temperature range of 23-27°c w ... | 2014 | 25075933 |
| transgenic cry1c or cry2a rice has no adverse impacts on the life-table parameters and population dynamics of the brown planthopper, nilaparvata lugens (hemiptera: delphacidae). | transgenic rice producing the insecticidal protein from bacillus thuringiensis berliner (bt) is protected from damage by lepidopteran insect pests. however, one of the main concerns about bt rice is the potential impact on non-target herbivores. in the present study, the ecological impacts of two bt rice lines, t1c-19 expressing cry1c protein and t2a-1 expressing cry2a protein, on the non-target herbivore brown planthopper (bph), nilaparvata lugens (stål), were evaluated under laboratory and fie ... | 2015 | 25067834 |
| contrasting effects of ethylene biosynthesis on induced plant resistance against a chewing and a piercing-sucking herbivore in rice. | ethylene is a stress hormone with contrasting effects on herbivore resistance. however, it remains unknown whether these differences are plant- or herbivore-specific. we cloned a rice 1-aminocyclopropane-1-carboxylic acid (acc) synthase gene, osacs2, whose transcripts were rapidly up-regulated in response to mechanical wounding and infestation by two important pests: the striped stem borer (ssb) chilo suppressalis and the brown planthopper (bph) nilaparvata lugens. antisense expression of osacs2 ... | 2014 | 25064847 |
| identification and characterization of micrornas in small brown planthopper (laodephax striatellus) by next-generation sequencing. | micrornas (mirnas) are endogenous non-coding small rnas that regulate gene expression at the post-transcriptional level and are thought to play critical roles in many metabolic activities in eukaryotes. the small brown planthopper (laodephax striatellus fallén), one of the most destructive agricultural pests, causes great damage to crops including rice, wheat, and maize. however, information about the genome of l. striatellus is limited. in this study, a small rna library was constructed from a ... | 2014 | 25057821 |
| physiological efects of paichongding applied to rice on the nilaparvata lugens (stå l), the brown planthopper. | nilaparvata lugens (stål) is a major rice pest in asia. paichongding is a novel neonicotinoid insecticide developed in 2008. the effects of this insecticide on the activity of detoxification enzymes of n. lugens and on rice resistance to the pest were examined in the laboratory. the results showed that paichongding could significantly decrease the acetylcholinesterase and gshs transferase activities of n. lugens. the variation tendency of mixed function oxidase (mfo) activity was similar with th ... | 2014 | 25042427 |
| bio-effects of near-zero magnetic fields on the growth, development and reproduction of small brown planthopper, laodelphax striatellus and brown planthopper, nilaparvata lugens. | magnetic fields markedly affect the growth and development of many species of organisms potentially due to cryptochrome and endogenous presence of magnetic materials. sensitivity to magnetic fields can also be involved in geomagnetic orientation by some long-distance migratory insects. in this study, near-zero magnetic fields (nzmf) in relation to normal geomagnetic fields (gmf) were setup using the hypomagnetic field space system (hmfs) to investigate the effects of magnetic fields on the growt ... | 2014 | 24995837 |
| chitin deacetylase family genes in the brown planthopper, nilaparvata lugens (hemiptera: delphacidae). | chitin deacetylases (cdas) are enzymes required for one of the pathways of chitin degradation, in which chitosan is produced by the deacetylation of chitin. bioinformatic investigations with genomic and transcriptomic databases identified four genes encoding cdas in nilaparvata lugens (nlcdas). phylogenetic analysis showed that insect cdas were clustered into five major groups. group i, iii and iv cdas are found in all insect species, whereas the pupa-specific group ii and gut-specific group v c ... | 2014 | 24989071 |