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ethylenediaminedissuccinate as a new chelate for environmentally safe enhanced lead phytoextraction.using a soil column experiment, we compared the effect of a single dose and weekly additions of ethylenediaminetetraacetic acid (edta) and ethylenediaminedissuccinate (edds) on the uptake of pb, zn, and cd by chinese cabbage [brassica rapa l. subsp. pekinensis (lour.) hanelt], and on the leaching of heavy metals through the soil profile. the analysis of plant material revealed that both chelates increased the concentrations of pb and, to a lesser extent, also of zn and cd in the leaves of the te ...201312708673
soil persistence of 4-hppd-inhibitors in different soil types.in field experiments carried out during the 1997-2001 period on four different soil types (sand, sandy loam, heavy sandy loam and clay) in flanders (belgium), the persistence of the three 4-hppd inhibiting maize herbicides mesotrione (100 and 150 g ha-1), sulcotrione (300 and 450 g ha-1) and isoxaflutole (75 and 125 g ha-1) was studied. therefore, soil samples were taken at regular intervals from application in spring and frozen. when all samples had been taken, greenhouse bioassays were set up ...200212696405
evaluation of the estrogenic effects of legume extracts containing phytoestrogens.seven legume extracts containing phytoestrogens were analyzed for estrogenic activity. methanol extracts were prepared from soybean (glycine max l.), green bean (phaseolus vulgaris l.), alfalfa sprout (medicago sativa l.), mung bean sprout (vigna radiata l.), kudzu root (pueraria lobata l.), and red clover blossom and red clover sprout (trifolium pratense l.). extracts of kudzu root and red clover blossom showed significant competitive binding to estrogen receptor beta (erbeta). estrogenic activ ...200312670155
the development of trichostrongylus colubriformis larvae on a range of herbage species or on plots of differing topographical aspect.five "contaminations", where faeces containing trichostrongylus colubriformis eggs were deposited on pasture and serially recovered, were used to compare the rate of decline of faecal mass and larval development. in the first three contaminations, faeces from a common source were deposited on swards of browntop (agrostis capillaris cv grasslands muster), ryegrass (lolium perenne cv grassland nui), white clover (trifolium pratense cv grassland tahora), or onto bare ground in the late spring, summ ...200312591198
a preliminary rapd-pcr analysis of cimicifuga species and other botanicals used for women's health.traditional taxonomic methods of botanical identification that rely primarily on morphological observations cannot be used efficiently when only powdered plant materials are available. thus, our objectives were to determine if we could apply a molecular approach to: a) produce unique dna profiles that are characteristic of the species, and b) determine if the geographical area or time of collection influences these dna profiles. towards this end, random amplified polymorphic dna (rapd) analyses ...200212587700
trifolium pratense (red clover) exhibits estrogenic effects in vivo in ovariectomized sprague-dawley rats.studies were conducted using an ovariectomized rat model to determine the estrogenic and antiestrogenic activity of trifolium pratense l. (red clover) extracts. a red clover extract, standardized to contain 15% isoflavones was administered by gavage [250, 500 and 750 mg/(kg x d)] to virgin, ovariectomized 50-d-old sprague-dawley rats, for 21 d in the presence and absence of 17beta-estradiol [50 microg/(kg x d)]. estrogenic effects included an increase in uterine weight, vaginal cell cornificatio ...200211773503
screening botanical extracts for quinoid metabolites.botanical dietary supplements represent a significant share of the growing market for alternative medicine in the usa, where current regulations do not require assessment of their safety. to help ensure the safety of such products, an in vitro assay using pulsed ultrafiltration and lc-ms-ms has been developed to screen botanical extracts for the formation of electrophilic and potentially toxic quinoid species upon bioactivation by hepatic cytochromes p450. rat liver microsomes were trapped in a ...200111712913
influence of the zinc hyperaccumulator thlaspi caerulescens j. & c. presl. and the nonmetal accumulator trifolium pratense l. on soil microbial populations.metal hyperaccumulator plants like thlaspi caerulescens j. & c. presl. are used for phytoremediation of contaminated soils. since little is known about the rhizosphere of hyperaccumulators, the influence of t. caerulescens was compared with the effects of trifolium pratense l. on soil microbes. high- and low-metal soils were collected near a zinc smelter in palmerton, penn. soil ph was adjusted to 5.8 and 6.8 by the addition of ca(oh)2. liming increased bacterial populations and decreased metal ...200111575505
red clover (trifolium pratense) for menopausal women: current state of knowledge.red clover (trifolium pratense) extracts are becoming increasingly popular, primarily for the treatment of menopausal symptoms. although promoted as a phytoestrogen source similar to soybeans, red clover is a medicinal herb, not a food, and traditionally has not been used long-term. we sought to review the scientific literature for this newer use.200611528359
evaluation of estrogenic activity of plant extracts for the potential treatment of menopausal symptoms.eight botanical preparations that are commonly used for the treatment of menopausal symptoms were tested for estrogenic activity. methanol extracts of red clover (trifolium pratense l.), chasteberry (vitex agnus-castus l.), and hops (humulus lupulus l.) showed significant competitive binding to estrogen receptors alpha (er alpha) and beta (er beta). with cultured ishikawa (endometrial) cells, red clover and hops exhibited estrogenic activity as indicated by induction of alkaline phosphatase (ap) ...200111368622
an in vitro evaluation of human cytochrome p450 3a4 inhibition by selected commercial herbal extracts and tinctures.serial dilutions of 21 commercial ethanolic herbal extracts and tinctures, and 13 related pure plant compounds have been analyzed for their in vitro cytochrome p450 3a4 (cyp3a4) inhibitory capability via a fluorometric microtitre plate assay. roughly 75% of the commercial products and 50% of the pure compounds showed significant inhibition of cyp3a4 metabolite formation. for each herbal product and pure compound exhibiting dose-dependency, the inhibition values were used to generate median inhib ...200010969720
forage systems for production of stocker steers in the upper south.the southern states produce large numbers of beef calves that are generally weaned and sold in autumn. keeping calves in this region beyond weaning to graze high-quality forages through a stocker cattle phase could improve profitability. autumn-weaned angus crossbred steers were allocated by breeding and weight to four forage systems that began in mid-november and continued through mid-october as follows: system 1, tall fescue (festuca arundinacea schreb.) and kentucky bluegrass (poa pratensis l ...200010907841
characterization of an atrazine-degrading pseudaminobacter sp. isolated from canadian and french agricultural soils.atrazine, a herbicide widely used in corn production, is a frequently detected groundwater contaminant. fourteen bacterial strains able to use this herbicide as a sole source of nitrogen were isolated from soils obtained from two farms in canada and two farms in france. these strains were indistinguishable from each other based on repetitive extragenic palindromic pcr genomic fingerprinting performed with primers eric1r, eric2, and boxa1r. based on 16s rrna sequence analysis of one representativ ...200010877767
ileal amino acid digestibilities in pigs of barley-based diets with inclusion of lucerne (medicago sativa), white clover (trifolium repens), red clover (trifolium pratense) or perennial ryegrass (lolium perenne).two experiments were performed with post-valve t-cannulated growing pigs, using five animals in each experiment in a change-over design to evaluate the effect of inclusion of four different dried forage meals on ileal crude protein (cp) and amino acid (aa) digestibilities. the control diets (c1 and c2) were barley-based and the experimental diets were formulated by replacing the barley with 100 or 200 g/kg of either lucerne (medicago sativa) or white clover (trifolium repens) meal in expt 1 and ...199910743486
molecular characterization and symbiotic importance of prsd gene of rhizobium leguminosarum bv. trifolii ta1.the prsd, prse and orf3 genes of rhizobium leguminosarum bv. trifolii strain ta1 encode the proteins which are significantly related to the family of bacterial abc transporters type i secretion systems. the prsd:km(r) mutant of strain ta1 induced non-nitrogen-fixing nodules on trifolium pratense. microscopic analysis of the nodules induced by prsd mutant did not reveal major abberations in the bacteroid appearance. the exopolysaccharide of prsd mutant was produced in increased amount and its lev ...199810397353
molecular characterization of psscde genes of rhizobium leguminosarum bv. trifolii strain ta1: pssd mutant is affected in exopolysaccharide synthesis and endocytosis of bacteria.we have identified the three genes psscde in rhizobium leguminosarum bv. trifolii ta1. even though they were almost identical to earlier identified psscde genes of r. leguminosarum, they differed in gene lengths and gene overlaps. the predicted gene products of psscde genes shared significant homology to prokaryotic glycosyl transferases involved in exopolysaccharide synthesis. the tn5 insertion in pssd created the nonmucoid mutant that induced non-nitrogen-fixing nodules. the microscopic analys ...19989805402
parasites and flower choice of bumblebees.in a field experiment, we tested whether workers of bumblebees, bombus pascuorum and b. humilis, parasitized by larvae of conopid flies, physocephala rufipes and sicus ferrugineus, differ in their flower choice from unparasitized ones. we collected workers at random in the field and immediately tested them in experimental arenas that offered the choice of a reference plant (red clover, trifolium pratense) versus a test plant (from five species). the choices of 396 workers were analysed with logi ...19989632470
antioxidant defenses in the peripheral cell layers of legume root nodules.ascorbate peroxidase (ap) is a key enzyme that scavenges potentially harmful h2o2 and thus prevents oxidative damage in plants, especially in n2-fixing legume root nodules. the present study demonstrates that the nodule endodermis of alfalfa (medicago sativa) root nodules contains elevated levels of ap protein, as well as the corresponding mrna transcript and substrate (ascorbate). enhanced ap protein levels were also found in cells immediately peripheral to the infected region of soybean (glyci ...19989449834
performance of lactating dairy cows fed red clover or alfalfa silage.the objective of this study was to compare utilization of red clover (trifolium pratense l.) and alfalfa (medicago sativa l.) by lactating dairy cows. red clover and alfalfa were harvested and conserved as silage at two maturities in 2 consecutive yr. each year, diets containing experimental forages and supplemental grain were fed to 16 multiparous holstein cows in early lactation in a replicated 4 x 4 latin square lactation trial. lactation performance and nutrient intake responses caused by fo ...19979436113
the in vitro biosynthesis of the exopolysaccharide produced by rhizobium leguminosarum bv. trifolii, strain na 30.rhizobium leguminosarum bv. trifolii, strain na 30 nodulates both red (trifolium pratense) and white (t. repens) clover and produces an acidic exopolysaccharide (eps) containing glucose, galactose, glucuronic acid, acetate and ketalpyruvate residues in a 5:1:2:1:2 molar ratio. the in vitro synthesis of this eps as well as the characterization of five structurally related lipid linked oligosaccharides is described employing edta treated cells as enzyme preparation and 14c-labelled udp-glc, udp-gl ...19968832106
forage systems for beef production from conception to slaughter: iii. finishing systems.fall-weaned angus calves grazed or were fed different forages during winter followed by 1) n-fertilized tall fescue (festuca arundinacea schreb.) grazed alone, 2) bluegrass (poa pratensis l.)-white clover (trifolium repens l.) sequence grazed with tall fescue-red clover (trifolium pratense l.), or 3) bluegrass-white clover sequence grazed with alfalfa (medicago sativa l.)-orchardgrass (dactylis glomerata l.). heifers were supplemented with grain at 1% of bw from april until slaughter in july. on ...19968707721
a hierarchical analysis of population genetic structure in rhizobium leguminosarum bv. trifolii.little is known about the population processes that shape the genetic diversity in natural populations of rhizobia. a sample of 912 rhizobium leguminosarum biovar trifolii isolates were collected from naturalized red clover populations (trifolium pratense) and analysed for 15 allozyme loci to determine the levels and distribution of genetic diversity. hierarchical analyses compared different sampling levels, geographical separation, and temporal separation. total genetic diversity across all iso ...19968673267
a central domain of rhizobium node protein mediates host specificity by determining the hydrophobicity of fatty acyl moieties of nodulation factors.previously, we have shown that the node gene is a major determinant of the difference in host range between rhizobium leguminosarum biovars viciae and trifolii. a new genetic test system for stringent functional analysis of node genes was constructed. by testing chimeric node genes constructed by the exchange of polymerase chain reaction (pcr)-generated restriction cassettes, we show that a central domain, containing only 44 non-conserved amino acid residues, determines the host specificity of t ...19958577248
evaluation of brassicas in grazing systems for sheep: i. quality of forage and animal performance.four years of grazing trials were conducted with brassica forages to evaluate their chemical composition and effect on adg of fattening lambs and breeding ewes in late fall. brassicas tested included kales (brassica oleracea l. var. acephala dc), turnips (b. rapa l.), and a chinese cabbage hybrid (b. rapa l. x b. pekinensis [lour.] rupr.). daily gains of lambs varied widely among years (19 to 330 g/d); adg on brassicas were, however, generally higher than on stockpiled kentucky 31 tall fescue (f ...19947928762
oviposition and development of face flies in dung from cattle on herbage and supplemented herbage diets.dung was collected from angus cattle (bos taurus l.) fed (ad libitum) hays of endophyte-free (ef) and endophyte (acremonium coenophialum morgan-jones and gams) infected (ei) tall fescue (festuca arundinacea schreb.), smooth bromegrass (bromus inermis leyss.), red clover (trifolium pratense l.), alfalfa (medicago sativa l.), and alfalfa-smooth bromegrass (1:1 w/w) and green-chopped kentucky bluegrass (poa pratensis l.). samples of dung were subsequently collected from the same animals offered the ...19947886909
a soap technique for cell separation to study the seed coat of sesbania punicea.a technique is described for separating plant cells used for morphological studies. the plant material is placed in a concentrated solution of olive oil castile soap for 1-2 days or more. the material is then thoroughly washed and placed between two glass slides. the upper glass slide is lifted from the lower one, then gently pressed down several times. through this procedure malpighian cells of the seed coat of sesbania punicea, mesophyll cells of euphorbia peplus and of trifolium pratense and ...19937687883
in vitro digestion of bloat-safe and bloat-causing legumes by rumen microorganisms: gas and foam production.leaves of three bloat-safe legumes -- birdsfoot trefoil (lotus corniculatus l.), sainfoin (onobrychis viciaefolia scop.), and cicer milkvetch (astralagus cicer l.) -- and of three bloat-causing legumes -- alfalfa (medicago sativa l.), red clover (trifolium pratense l.), and white clover (trifolium repens l.) -- were incubated with strained rumen fluid or with mixed rumen fluid and solids. gas released was measured during the early period (0 to 22 h) of this in vitro digestion. gas volume was gre ...19807419778
transfer of rhizobium meliloti psym genes into agrobacterium tumefaciens: host-specific nodulation by atypical infection.the psym megaplasmid of rhizobium meliloti 2011 mobilized by plasmid rp4, or plasmid pgmi42, an rp4-prime derivative which carries a 290-kilobase psym fragment including nitrogenase and nod genes, was introduced into agrobacterium tumefaciens. the resulting transconjugants induced root deformations specifically on the homologous hosts medicago sativa and melilotus alba and not on the heterologous hosts trifolium pratense and trifolium repens. the root deformations were shown to be genuine nodule ...19846690420
[sodium, zinc and managanese available to dairy cows grazing on weathered soils of different geological origins, as determined by analysis of the black hairs of the cattle, and of hops and purple clover]. 19715556570
electron microscopy of the development of erysiphe polygoni in resistant and susceptible trifolium pratense. 19695377739
formation of tumor-like structures on legume roots by rhizobium.tumor-like structures appeared on the roots of medicago sativa, alysicarpus vaginalis, and trifolium pratense inoculated with a non-nodulating strain of rhizobium trifolii or with irradiated cultures of either of two nodulating rhizobium strains. the structures were composed of disorganized plant tissues which, on the basis of microscopic examination, were devoid of bacterial cells. rhizobia which could nodulate legumes of one cross-inoculation group and which were able to induce formation of su ...19714995381
the role of condensed tannins in the nutritional value of lotus pedunculatus for sheep. rates of body and wool growth.lotus pedunculatus (cv. grasslands maku) grown on acid low-fertility soil and containing high concentrations of condensed tannin (76-90 g/kg dry matter (dm] was grazed by growing sheep for 31-42 d periods in three experiments. in expt 2 an additional group of lambs grazed areas oversown with white clover (trifolium repens) and red clover (trifolium pratense). lambs were transferred from grazing ryegrass (lolium perenne)-white clover straight on to lotus in all experiments (unconditioned sheep). ...19854063306
the role of condensed tannins in the nutritional value of lotus pedunculatus for sheep. 5. effects on the endocrine system and on adipose tissue metabolism.1. three experiments were conducted using lotus pedunculatus containing high concentrations of condensed tannins (ct), and utilizing the principle that polyethylene glycol (peg) application (molecular weight 3350) will irreversibly bind a portion of the ct and thus reduce the dietary reactive (i.e. non-peg bound) ct concentration. lotus diets containing 95, 45 and 14 g total reactive ct/kg dry matter (dm), induced by spraying with three peg rates, were given to sheep at hourly intervals (600 g d ...19863676235
cryptic viruses in hop trefoil (medicago lupulina) and their relationships to other cryptic viruses in legumes.three double-stranded (ds) rna-containing viruses, named hop trefoil cryptic virus (htcv) 1, 2 and 3, were found in apparently healthy seedlings of hop trefoil (medicago lupulina). only 1-2% of plants tested were virus-free. the particles occurred in the cytoplasm, nuclei and nucleoli of parenchyma cells. they were purified as a mixture (htcv-m) with a mean yield of 50-100 micrograms/kg fresh tissue, and an antiserum was obtained. htcv1 was serologically related to one component (acv1) of a mixt ...19873450677
creep grazing and early weaning effects on cow and calf productivity.one hundred fifty simmental-hereford cows and calves were used in a 3-yr study to evaluate three creep grazing treatments and an early weaning treatment on cow and calf performance during midsummer (july to september). calves were approximately 150 d of age and averaged 178.6 kg when treatments were initiated. tifleaf pearl millet (pennisetum americanum l. leeke) was used as the forage for two of the creep treatments, representing two cow stocking intensities of .466 (tlm1) and .239 (tlm2) ha of ...19883397337
monensin controlled-release intraruminal capsule for control of bloat in pastured dairy cows.monensin, a polyether ionophore antibiotic, is potentially an important agent for bloat relief in dairy cows grazing temperate legume-based pasture. a series of studies was undertaken to determine the effect of monensin, when delivered continuously in the rumen of lactating dairy cows by means of controlled-release capsules (monensin crc). such devices release approximately 300 mg/head/day for 100 d. a short-term pilot study made at ruakura, new zealand, tested monensin crc in cows selected for ...19912018451
effects of synthetic and naturally occurring flavonoids on benzo[a]pyrene metabolism by hepatic microsomes prepared from rats treated with cytochrome p-450 inducers.activity-directed fractionation of trifolium pratense resulted in isolation of the isoflavone biochanin a, a potent inhibitor of metabolic activation of the carcinogen benzo[a]pyrene (b[a]p) in cells in culture. to determine the structural features required for maximal inhibition of cytochrome p-450 mediated metabolism of b[a]p, the inhibitory potencies of 23 flavonoids on metabolism of b[a]p to water-soluble derivatives were examined in liver s-9 homogenate from rats induced with aroclor 1254. ...19911913623
monitoring by epifluorescence microscopy of organelle dna fate during pollen development in five angiosperm species.the fates of mitochondrial and plastid nucleoids during pollen development in six angiosperm species (antirrhinum majus, glycine max, medicago sativa, nicotiana tabacum, pisum sativum, and trifolium pratense) were examined using epifluorescence microscopy after double staining with 4',6-diamidino-2- phenylindole (dapi) to stain dna and with a potentiometric dye (either dioc7 or rhodamine 123) for visualization of metabolically active mitochondria. from the pollen mother cell stage to the microsp ...19911879613
forage systems for beef production from conception to slaughter: i. cow-calf production.six year-round, all-forage, three-paddock systems for beef cow-calf production were used to produce five calf crops during a 6-yr period. forages grazed by cows during spring, summer, and early fall consisted of one paddock of 1) tall fescue (festuca arundinacea schreb.)-ladino clover (trifolium repens l.) or 2) kentucky blue-grass (poa pratensis l.)-white clover (trifolium repens l.). each of these forage mixtures was combined in a factorial arrangement with two paddocks of either 1) fescue-red ...19921548221
forage systems for beef production from conception to slaughter: ii. stocker systems.fall weaned angus calves grazed stockpiled 1) tall fescue (festuca arundinacea schreb.), 2) tall fescue-red clover (trifolium pratense l.), or 3) tall fescue-alfalfa (medicago sativa l.) or were barn-fed, 4) tall fescue hay, 5) orchardgrass (dactylis glomerata l.)-alfalfa hay, or 6) tall fescue silage from late october to early april during each of 5 yr. infection of the fescue with acremonium coenophialum ranged from 0 to 55%. there were two replications each of steers and heifers for each fora ...19921312526
microbial changes in clover rhizosphere after foliar and soil application of cobalt.application of cobalt(ii) nitrate to the leaves of red clover (trifolium pratense l.) resulted in a pronounced increase of dry weight and the number of root nodules. counts of bacteria in the rhizosphere, content of ammonia and production of carbon dioxide in rhizosphere soil were also higher, whereas the content of nitrates decreased. differences in the counts of bacteria, actinomycetes, azotobacter, anaerobic bacteria and cellulose decomposing bacteria in the rhizosphere of control and treated ...1978669491
[biological fertilization (mycorrhiza + rhizobium + phosphobacteria) of trifolium pratense in different cultural conditions]. 1979293006
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