Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| a controllable bacterial lysis system to enhance biological safety of live attenuated vibrio anguillarum vaccine. | bacterial strains used as backbone for the generation of vaccine prototypes should exhibit an adequate and stable safety profile. given the fact that live attenuated vaccines often contain some potential risks in virulence recovery and spread infections, new approaches are greatly needed to improve their biological safety. here, a critically iron-regulated promoter pviua was screened from vibrio anguillarum, which was demonstrated to respond to iron-limitation signal both in vitro and in vivo. b ... | 2015 | 26052008 |
| characterization and evolutionary analysis of duplicated c7 in miiuy croaker. | the complement system, as one of the most sophisticated innate immune system, plays an important role in defense against invading microorganisms. the complement component c7 participates in the cytolytic phase of complement activation through a series of polymerization reactions with other terminal complement components. in this study, we derived two c7 genes from the whole genome of miiuy croaker which were the consequence of the fish-specific genome duplication. our data showed that miiuy croa ... | 2015 | 26036969 |
| involvement of relish gene from macrobrachium rosenbergii in the expression of anti-microbial peptides. | relish is an nf-kb transcription factor involved in immune-deficiency (imd) signal pathway. in this study, a relish gene (mrrelish) was identified from macrobrachium rosenbergii. the full length of mrrelish comprises 5072 bp, including a 3510 bp open reading frame encoding a 1169 bp amino acid protein. mrrelish contains a rel homology domain (rhd), a nucleus localization signal, an iκb-like domain (6 ankyrin repeats), and a death domain. phylogenetic analysis showed that mrrelish and other relis ... | 2015 | 26026243 |
| screening for antibacterial activity of some turkish plants against fish pathogens: a possible alternative in the treatment of bacterial infections. | the antibacterial activity of ethanolic and aqueous crude extracts from 36 plants in turkey, including seven endemic species, against fish pathogens was studied using the disc diffusion assay. the extract that was most active against all microbial strains, except aeromonas salmonicida, was that of dorycnium pentaphyllum. some of the extracts also showed a very broad spectrum of potent antimicrobial activity. the extract of anemone nemorosa showed the highest antimicrobial activity against vibrio ... | 2015 | 26019642 |
| molecular characterization of a transmembrane c-type lectin receptor gene from ayu (plecoglossus altivelis) and its effect on the recognition of different bacteria by monocytes/macrophages. | c-type lectin receptors (ctlrs) play vital roles in immune responses as pattern-recognition receptors (prrs). in this study, we identified a novel c-type lectin receptor (pactlrc) gene from ayu, plecoglossus altivelis. predicted pactlrc is a single transmembrane receptor with a typical carbohydrate recognition domain (crd) at its c-terminus. sequence comparison and phylogenetic tree analysis showed that pactlrc was most closely related to atlantic salmon (salmo salar) clrc, but was significantly ... | 2015 | 26010409 |
| a novel integrin function in innate immunity from chinese mitten crab (eriocheir sinensis). | integrins belong to a superfamily of conserved α β heterodimeric cell surface receptors that have critical function in cell migration, differentiation, and survival. in this study, an integrin called esintegrin was identified from chinese mitten crab eriocheir sinensis. esintegrin cdna is 4415 bp long with a 2457 bp open reading frame that encodes an 818 amino acid protein. esintegrin contains a signal peptide, an integrin beta subunit (n-terminal portion of extracellular region) inb domain, an ... | 2015 | 26004499 |
| growth inhibition of bacterial fish pathogens and quorum-sensing blocking by bacteria recovered from chilean salmonid farms. | the main goal of this study was to find bacterial isolates with the ability to inhibit the growth of the fish pathogens aeromonas hydrophila, vibrio anguillarum, and flavobacterium psychrophilum and to inhibit the blockage of the quorum-sensing (qs) system. a total of 80 gram-negative strains isolated from various freshwater chilean salmonid farms were studied. we determined that 10 strains belonging to the genus pseudomonas inhibited at least one of the assayed fish pathogens. of these, nine st ... | 2015 | 26000731 |
| the antimicrobial activity of heterotrophic bacteria isolated from the marine sponge erylus deficiens (astrophorida, geodiidae). | interest in the study of marine sponges and their associated microbiome has increased both for ecological reasons and for their great biotechnological potential. in this work, heterotrophic bacteria associated with three specimens of the marine sponge erylus deficiens, were isolated in pure culture, phylogenetically identified and screened for antimicrobial activity. the isolation of bacteria after an enrichment treatment in heterotrophic medium revealed diversity in bacterial composition with o ... | 2015 | 25999928 |
| molecular characterization and immune response expression of the qm gene from the scallop chlamys farreri. | the scallop chlamys farreri is an important aquaculture species in northern china. however, the sustainable development of the scallop industry is currently threatened by several pathogens that cause mass mortality of this mollusk. therefore, a complete understanding of the immune response mechanisms involved in host-virus interactions is necessary. this study reports a novel qm gene from c. farreri. this gene was first identified as a putative tumor suppressor gene from human and then confirmed ... | 2015 | 25982398 |
| a manganese superoxide dismutase (mnsod) from ark shell, scapharca broughtonii: molecular characterization, expression and immune activity analysis. | manganese superoxide dismutase (mnsod) is one of the key members of the antioxidant defense enzyme family, however, data regarding to the immune function of mnsod in mollusks still remain limited now. in this study, a full-length mnsod cdna was identified by rapid amplification of cdna ends (race) method from cdna library of ark shell scapharca broughtonii (termed sbmnsod). the cdna contained an open reading frame (orf) of 696 bp which encoded a polypeptide of 232 amino acids, a 5'-utr with leng ... | 2015 | 25980798 |
| pathogenicity of members of the vibrionaceae family to cultured juvenile sablefish. | sablefish anoplopoma fimbria are a prized seafood species due to their high oil content and white flaky flesh. raising these species in culture can help to provide an important source of protein for humans and relief to declining wild fish populations. understanding the environmental factors that influence the production of sablefish is important for successful culturing. the significance of host-pathogen interactions in sablefish culture and the resulting environmental implications are unknown. ... | 2015 | 25970236 |
| profiling immune response in zebrafish intestine, skin, spleen and kidney bath-vaccinated with a live attenuated vibrio anguillarum vaccine. | 2015 | 25956722 | |
| dietary exposure to individual polybrominated diphenyl ether congeners bde-47 and bde-99 alters innate immunity and disease susceptibility in juvenile chinook salmon. | polybrominated diphenyl ethers (pbdes), used as commercial flame-retardants, are bioaccumulating in threatened pacific salmon. however, little is known of pbde effects on critical physiological functions required for optimal health and survival. bde-47 and bde-99 are the predominant pbde congeners found in chinook salmon collected from the pacific northwest. in the present study, both innate immunity (phagocytosis and production of superoxide anion) and pathogen challenge were used to evaluate h ... | 2015 | 25938634 |
| evolution of akirin family in gene and genome levels and coexpressed patterns among family members and rel gene in croaker. | akirins, which are highly conserved nuclear proteins, are present throughout the metazoan and regulate innate immunity, embryogenesis, myogenesis, and carcinogenesis. this study reports all akirin genes from miiuy croaker and analyzes comprehensively the akirin gene family combined with akirin genes from other species. a second nuclear localization signal (nls) is observed in akirin2 homologues, which is not in akirin1 homologues in all teleosts and most other vertebrates. thus, we deduced that ... | 2015 | 25912355 |
| vibriophages differentially influence biofilm formation by vibrio anguillarum strains. | vibrio anguillarum is an important pathogen in marine aquaculture, responsible for vibriosis. bacteriophages can potentially be used to control bacterial pathogens; however, successful application of phages requires a detailed understanding of phage-host interactions under both free-living and surface-associated growth conditions. in this study, we explored in vitro phage-host interactions in two different strains of v. anguillarum (ba35 and pf430-3) during growth in microcolonies, biofilms, and ... | 2015 | 25911474 |
| identification, expression pattern and potential role of variable lymphocyte receptor aj-vlra from apostichopus japonicus in response to bacterial challenge. | the variable lymphocyte receptors (vlrs) are found in jawless vertebrates (agnathans), and specifically recognize bacteria and viruses via their leucine-rich repeats (lrrs). vlrs are believed to be adaptive immune response molecules. echinoderms do not have adaptive immune systems; however, in the present study, a vlr cdna named aj-vlra was cloned and characterized from sea cucumber, apostichopus japonicus. the complete cdna of aj-vlra was 3072 bp, including a 1995 bp open reading frame encoding ... | 2015 | 25896798 |
| a c-type lectin fold gene from japanese scallop mizuhopecten yessoensis, involved with immunity and metamorphosis. | c-type lectins are a superfamily of ca(2+)-dependent carbohydrate-recognition proteins that are well known for their participation in pathogen recognition and clearance. in this study, a putative c-type lectin fold (myclf) gene was identified from the japanese scallop mizuhopecten yessoensis. the full-length of myclf was 645 bp, encoding a polypeptide of 167 amino acids. myclf carried a signal peptide of 20 amino acid residues, and a single carbohydrate recognition domain, having relatively high ... | 2015 | 25867372 |
| molecular characterization and functional analysis of a novel c-type lectin receptor-like gene from a teleost fish, plecoglossus altivelis. | c-type lectin-like receptors (clrs) are important pathogen pattern recognition molecules that recognize carbohydrate structures. however, the functions of these receptors in fish keep less known. in this study, we characterized a novel clr from a teleost fish, plecoglossus altivelis (ayu), tentatively named pacd209l. the cdna of pacd209l is 1464 nucleotides (nts) in length, encoding a polypeptide of 281 amino acid residues with a calculated molecular weight of 31.5 kda. multiple alignment of the ... | 2015 | 25842180 |
| characterizations and expression analyses of nf-κb and rel genes in the yesso scallop (patinopecten yessoensis) suggest specific response patterns against gram-negative infection in bivalves. | rel/nf-κb (nuclear factor kappa-light-chain-enhancer of activated b cells) genes are evolutionarily conserved and play a pivotal role in several physiological events. they have been extensively studied from various species, including both vertebrates and invertebrates. however, the rel/nf-κb genes have not been systematically characterized in bivalves. in this study, we identified and characterized pynf-κb and pyrel in the yesso scallop (patinopecten yessoensis). phylogenetic and protein structu ... | 2015 | 25842178 |
| a galectin from shrimp litopenaeus vannamei is involved in immune recognition and bacteria phagocytosis. | galectins are conserved family members with β-galactosides affinity that play multiple functions in embryogenesis, development and regulation of innate and adaptive immunity. however, little functional studies were reported in crustaceans. here, a shrimp litopenaeus vannamei galectin (lvgal) cdna was identified with an open reading frame of 1017 bp, which encodes a putative protein of 338 amino acids. a carbohydrate recognition domain (crd) and several amino acids residues involved in dimerizati ... | 2015 | 25819117 |
| relation between virulence of vibrio anguillarum strains and response to the host factors mucin, bile salts and cholesterol. | in this study, we investigated the responsiveness of 15 vibrio anguillarum strains to three host factors (mucin, bile salts and cholesterol). | 2015 | 25807847 |
| a single-crd c-type lectin from oyster crassostrea gigas mediates immune recognition and pathogen elimination with a potential role in the activation of complement system. | c-type lectins (ctls), serving as pattern recognition receptors (prrs), are a superfamily of ca(2+)-dependent carbohydrate-recognition proteins that participate in nonself-recognition and pathogen elimination. in the present study, a single carbohydrate-recognition domain (crd) ctl was identified from oyster crassostrea gigas (designated as cgclec-2). there was only one crd within the deduced amino acid sequence of cgclec-2 consisting of 129 amino acid residues. a conserved epn (glu246-pro247-as ... | 2015 | 25800112 |
| comparative evaluation of infection methods and environmental factors on challenge success: aeromonas salmonicida infection in vaccinated rainbow trout. | when testing vaccine-induced protection an effective and reliable challenge method is a basic requirement and we here present a comparative study on different challenge methods used for infection of rainbow trout oncorhynchus mykiss with aeromonas salmonicida, a bacterial pathogen eliciting furunculosis. fish were vaccinated with three different adjuvanted trivalent vaccines containing formalin killed a. salmonicida, vibrio anguillarum o1 and o2a. these were 1) the commercial vaccine alpha ject ... | 2015 | 25783001 |
| genome-wide snp identification for the construction of a high-resolution genetic map of japanese flounder (paralichthys olivaceus): applications to qtl mapping of vibrio anguillarum disease resistance and comparative genomic analysis. | high-resolution genetic maps are essential for fine mapping of complex traits, genome assembly, and comparative genomic analysis. single-nucleotide polymorphisms (snps) are the primary molecular markers used for genetic map construction. in this study, we identified 13,362 snps evenly distributed across the japanese flounder (paralichthys olivaceus) genome. of these snps, 12,712 high-confidence snps were subjected to high-throughput genotyping and assigned to 24 consensus linkage groups (lgs). t ... | 2015 | 25762582 |
| rescue of fish exposed to a lethal dose of pathogen, by signals from sublethally exposed survivors. | rainbow trout (oncorhynchus mykiss, walbaum) were challenged intraperitoneally with a sublethal dose of vibrio anguillarum vib1 and allowed to recover. then, after 7 days, naïve fish, (designated as 'bystander' fish) which had never been exposed to the pathogen, were introduced to the same tank. these swam with the adapted (recovered) fish for 7 days before both groups and a control (never exposed directly to the pathogen or to recovered fish) group were exposed to a lethal dose of vib1. mortali ... | 2015 | 25757731 |
| molecular characterization of the liver-expressed antimicrobial peptide 2 (leap-2) in a teleost fish, plecoglossus altivelis: antimicrobial activity and molecular mechanism. | liver-expressed antimicrobial peptide 2 (leap-2) is widespread in fish and plays an important role in the host's innate immune system. in this study, full-length cdna for leap-2 (paleap-2) gene was cloned and sequenced from ayu, plecoglossus altivelis. paleap-2 mrna was detected in a wide range of tissues, with the highest level of transcripts found in the liver. upon induction by vibrio anguillarum, its expression significantly increased in the liver, kidney, spleen, gill, and heart, but decrea ... | 2015 | 25749706 |
| isolation and characterization of a 17-kda fkbp-type peptidyl-prolyl cis/trans isomerase from vibrio anguillarum. | peptidyl-prolyl cis/trans isomerase (ppiase) catalyzes the isomerization of peptide bonds to achieve conformational changes in native folded proteins. an fkbp-type ppiase with an approximate molecular weight of 17kda was isolated from vibrio anguillarum o1 and named vafkbp17. to investigate its biochemical properties, the ppi gene from v. anguillarum o1 was isolated and overexpressed in escherichia coli. a protease-coupled assay for isomerization activity, using succinyl-ala-phe-pro-phe-p nitroa ... | 2015 | 25747528 |
| syntenin is involved in the bacteria clearance response of kuruma shrimp (marsupenaeus japonicus). | syntenin is a multifunctional cytosolic adaptor protein that contributes to cell migration, proliferation, attachment, and apoptosis, as well as immune response to virus, in vertebrates. however, the functions of syntenin in the antibacterial response of invertebrates remain unclear. in this study, we identified a syntenin-like gene (mjsyn) from the kuruma shrimp (marsupenaeus japonicus) and detected its function in the antibacterial immunity of shrimp. the full-length mjsyn was 1223 bp with a 9 ... | 2015 | 25731918 |
| expression profiling analysis of the microrna response of cynoglossus semilaevis to vibrio anguillarum and other stimuli. | to investigate the roles of micrornas (mirna) of cynoglossus semilaevis in response to vibrio anguillarum that were previously identified using high-throughput sequencing, microarray analyses was performed on three small rna libraries (cg, nosg, and hosg) prepared from c. semilaevis immune tissues. in total, of 1279 designed probes, 739 (57.78 %) were detectable. the expression levels of these mirnas were analyzed using pairwise comparisons among the three libraries, and a total of 99 mirnas wer ... | 2015 | 25715708 |
| effects of transportation stress and addition of salt to transport water on the skin mucosal homeostasis of rainbow trout (oncorhynchus mykiss). | transportation of live fish is a common practice among aquaculture facilities. many studies have previously reported how transport elicits physiological stress responses and increases disease susceptibility in farmed fish. the aim of this work is to investigate the changes that the skin of rainbow trout (oncorhynchus mykiss) experiences due to stress. since nacl is commonly added to transport water as a stress mitigator, the effects of salt addition on the skin mucosa and skin-associated bacteri ... | 2015 | 25705060 |
| comparison of immune response of pacific white shrimp, litopenaeus vannamei, after multiple and single infections with wssv and vibrio anguillarum. | our previous study demonstrated that pacific white shrimp (litopenaeus vannamei) infected by multiple pathogens showed higher mortality and death occurred more quickly than those infected by a single pathogen (jang et al., 2014). for better understanding the defense mechanism against white spot syndrome virus (wssv) and vibrio anguillarum, immune responses of shrimp were evaluated in this study. the mrna expression levels of five immune-related genes were analyzed by quantitative reverse real-ti ... | 2015 | 25700782 |
| a defensin from clam venerupis philippinarum: molecular characterization, localization, antibacterial activity, and mechanism of action. | antimicrobial peptides (amps) are important mediators of the primary host defense system against microbial invasion. in the present study, we cloned and characterized a member of the invertebrate defensin from the clam venerupis philippinarum, designated vpdef. amino acid sequence analysis showed that vpdef was similar to defensins from marine mollusks and ticks. in non-stimulated clams, rt-pcr and immunohistochemical analysis revealed that both vpdef mrna and the encoding peptide were constitut ... | 2015 | 25697801 |
| identification of the atlantic cod l-amino acid oxidase and its alterations following bacterial exposure. | antibacterial factors that are present in epidermal mucus of fish have a potential role in the first line of host defence to bacterial pathogens. this study reports the identification of l-amino acid oxidase (lao) in atlantic cod (gmlao) and the changes in the molecule following bacterial exposure. the gmlao transcripts and lao activity were present on both the body surface and in the internal organs of the fish. relative mrna level of gmlao increased significantly in the gills, the spleen and t ... | 2015 | 25681742 |
| identification of the target dna sequence and characterization of dna binding features of hlyu, and suggestion of a redox switch for hlya expression in the human pathogen vibrio cholerae from in silico studies. | hlyu, a transcriptional regulator common in many vibrio species, activates the hemolysin gene hlya in vibrio cholerae, the rtxa1 operon in vibrio vulnificus and the genes of plp-vah1 and rtxachbde gene clusters in vibrio anguillarum. the protein is also proposed to be a potential global virulence regulator for v. cholerae and v. vulnificus. mechanisms of gene control by hlyu in v. vulnificus and v. anguillarum are reported. however, detailed elucidation of the interaction of hlyu in v. cholerae ... | 2015 | 25605793 |
| four crustins involved in antibacterial responses in marsupenaeus japonicus. | crustins are a family of cationic, cysteine-rich antimicrobial peptides with a whey acidic protein (wap) domain in the c-terminal. they have diverse functions in antimicrobial immune responses. four groups of crustins (crustins i, ii, iii, and iv) have been identified in crustaceans, but type i crustins have not been reported in penaeid shrimp until now. in this study, we identified four crustins in kuruma shrimp marsupenaeus japonicus, and named them mjcrus i-2, 3, 4 and 5. these four crustins ... | 2015 | 25583545 |
| a novel vertebrates toll-like receptor counterpart regulating the anti-microbial peptides expression in the freshwater crayfish, procambarus clarkii. | toll-like receptors (tlrs) play an important role in regulation of anti-microbial peptides (amps) expression. a novel vertebrates tlr counterpart named pctoll, was firstly identified from the freshwater crayfish, procambarus clarkii. phylogenetic analysis showed that pctoll together with drosophila melanogaster and anopheles gambiae toll9 were clustered with human tolls. pctoll was mainly expressed in hepatopancreas and gills and it also could be detected in hemocytes, heart, stomach and intesti ... | 2015 | 25573502 |
| characterization of a gc1qr from the giant freshwater prawn, macrobrachium rosenbergii. | gc1qr, as a multicompartmental and a multifunctional protein, plays an important role in innate immunity. in this study, a gc1qr homolog (mrgc1qr) in the giant freshwater prawn, macrobrachium rosenbergii was identified. mrgc1qr, a 258-amino-acid polypeptide, shares high identities with gc1qr from other species. mrgc1qr gene was expressed in different tissues and was highest expressed in the hepatopancreas. in addition, the mrgc1qr transcript was significantly enhanced after 6 h of white spot syn ... | 2015 | 25555810 |
| an epd/wsd motifs containing c-type lectin from argopectens irradians recognizes and binds microbes with broad spectrum. | c-type lectins are a superfamily of ca(2+)-dependent carbohydrate-recognition proteins consisting of at least one carbohydrate-recognition domain (crd), which play significant roles in nonself-recognition and clearance of invaders. the immune function of a c-type lectin (aictl-7) with epd/wsd motifs from argopectens irradians was investigated in the present study. the recombinant protein of aictl-7 (raictl-7) could bind lps, pgn, mannan, yeast glucan and poly i:c in vitro, and displayed a broade ... | 2015 | 25555809 |
| use of gnotobiotic zebrafish to study vibrio anguillarum pathogenicity. | we evaluated the use of the gnotobiotic zebrafish system to study the effects of bacterial infection, and analyzed expression of genes involved in zebrafish innate immunity. using a gfp-labeled strain of vibrio anguillarum, we fluorescently monitored colonization of the zebrafish intestinal tract and used gene expression analysis to compare changes in genes involved in innate immunity between nongnotobiotic and gnotobiotic larvae. the experiments performed with the gnotobiotic zebrafish reveal n ... | 2015 | 25548877 |
| transcriptome analysis revealed changes of multiple genes involved in immunity in cynoglossus semilaevis during vibrio anguillarum infection. | half-smooth tongue sole (cynoglossus semilaevis) is one of the most valuable marine aquatic species in northern china. given to the rapid development of aquaculture industry, the c. semilaevis was subjected to disease-causing bacteria vibrio anguillarum. it therefore is indispensable and urgent to understand the mechanism of c. semilaevis host defense against v. anguillarum infection. in the present study, the extensively analysis at the transcriptome level for v. anguillarum disease in tongue s ... | 2015 | 25543033 |
| preliminary study on expression of antimicrobial peptides in european sea bass (dicentrarchus labrax) following in vivo infection with vibrio anguillarum. a time course experiment. | antimicrobial polypeptides (ampps) are humoral components of the vertebrates and invertebrates innate immune system. their potent broad spectrum antimicrobial activities have drawn the attention of the scientific community to their potential use not only as an alternative to antibiotics but also as functional targets for immunostimulants in order to enhance the host immunity. fish synthesize a great number of these peptides but in european sea bass, an important fish species in the mediterranean ... | 2015 | 25542381 |
| comparative genomic of the baff and baff-like genes and immune response to bacteria of miiuy croaker (miichthys miiuy). | as a member of the tumor necrosis factor (tnf) family, b cell activating factor (baff), also known as tnf ligand superfamily member 13b (tnf13b), playing a critical role in enhancing immune responses. baff is a central cytokine for b-cell survival, proliferation, maturation and immunoglobulin secretion. in the present study, we describe the identification of the miiuy croaker baff (designed mmbaff) and baff-like (designed mmbaff-like) genes. the cdna of mmbaff contains an open reading frame (orf ... | 2015 | 25542380 |
| a novel phagocytic receptor (cgnimc) from pacific oyster crassostrea gigas with lipopolysaccharide and gram-negative bacteria binding activity. | phagocytosis is an evolutionarily conserved process to ingest the invading microbes and apoptotic or necrotic corpses, playing vital roles in defensing invaders and maintenance of normal physiological conditions. in the present study, a new nimrod family phagocytic receptor with three egf-like domains was identified in pacific oyster crassostrea gigas (designated cgnimc). cgnimc shared homology with other identified multiple egf-like domain containing proteins. the mrna transcripts of cgnimc wer ... | 2015 | 25541078 |
| identification, expression and immunological responses to bacterial challenge following vaccination of blt1 gene from turbot, scophthalmus maximus. | leukotriene b4 (ltb4) is well known as a chemoattractant for leucocytes, recent studies also showed its involvement in adaptive immunity. the purpose of this work is to report the cloning, characterization and gene expression of leukotriene b4 receptor (blt1) in turbot (scophthalmus maximus), as well as the immunological response to challenge following vaccination with a live attenuated vaccine vibrio anguillarum mvav6203. the full cdna sequence of turbot blt1 was cloned. the open reading frame ... | 2015 | 25541026 |
| identification and expression analysis of cdna encoding chitinase-like protein (clp) gene in japanese scallop mizuhopecten yessoensis. | chitinase-like proteins (clp) are important members of the glycoside hydrolase family 18 (gh18) and are involved in growth control and remodeling processes. in this study, a clp transcript was isolated and sequenced from the japanese scallop (mizuhopecten yessoensis) after screening expressed sequence tags. the full-length complementary dna of m. yessoensis clp (my-clp1) was 1555 bp in length, consisting of a 75-bp 5'-untranslated region (utr), a 160-bp 3'-utr, and a 1320-bp open reading frame b ... | 2014 | 25526193 |
| cloning and expression of a transcription factor activator protein-1 (ap-1) member identified from manila clam venerupis philippinarum. | the transcription factor activator protein-1 (ap-1) proteins are implicated to play a major role in the regulation of numerous genes involved in the function and development of the immune system, cell differentiation, proliferation, apoptosis, etc. it can bind to promoter of its target genes in a sequence-specific manner to transactivate or repress them. in this study, the full-length cdna of an ap-1 was identified from venerupis philippinarum (denoted as vpap-1) by est analysis and race approac ... | 2015 | 25523090 |
| molecular cloning, expression, purification and characterization of vitellogenin in scallop patinopecten yessoensis with special emphasis on its antibacterial activity. | vitellogenin (vg), the major precursor of the egg-yolk proteins, has been found to play an immune role in fish and protochordate amphioxus, however, no study on the immune function of vg in invertebrates has ever been studied before. in this study, the complete cdna of vg was identified from the scallop patinopecten yessoensis (termed pyvg). the cdna contained an open reading frame (orf) of 6888 bp, encoding a polypeptide of 2295 amino acid protein, which had an n-terminal signal peptide followe ... | 2015 | 25499034 |
| identification and expression of antioxidant and immune defense genes in the surf clam mesodesma donacium challenged with vibrio anguillarum. | the immune system in marine invertebrates is mediated through cellular and humoral components, which act together to address the action of potential pathogenic microorganisms. in bivalve mollusks biomolecules implicated in oxidative stress and recognition of pathogens have been involved in the innate immune response. to better understand the molecular basis of the immune response of surf clam mesodesma donacium, qpcr approaches were used to identify genes related to its immune response against v ... | 2015 | 25481276 |
| a novel crustin from marsupenaeus japonicus promotes hemocyte phagocytosis. | crustins are cationic cysteine-rich antimicrobial peptides (amps) that contain multiple domains (glycine-rich, cysteine-rich, or proline-rich) at the n-terminus and whey acidic protein (wap) domains at the c-terminus. crustins have multiple functions, including protease inhibition and antimicrobial activity. other functions of crustins need to be clarified. in this study, a novel crustin with a cysteine-rich region, and a single wap domain, belonging to type i crustins, was identified in marsupe ... | 2015 | 25479014 |
| impact of mucin, bile salts and cholesterol on the virulence of vibrio anguillarum towards gnotobiotic sea bass (dicentrarchus labrax) larvae. | in this study, we investigated the impact of the host factors mucin, bile salts and cholesterol on the virulence of the economically important aquatic pathogen vibrio anguillarum towards sea bass larvae. pretreatment of v. anguillarum with either one of the host factors (at 10 mg l(-1)) prior to inoculation into the sea bass rearing water increased virulence of the bacterium, although the effect of cholesterol was not significant. each of the three host factors significantly increased several vi ... | 2015 | 25465176 |
| phage therapy as an approach to prevent vibrio anguillarum infections in fish larvae production. | fish larvae in aquaculture have high mortality rates due to pathogenic bacteria, especially the vibrio species, and ineffective prophylactic strategies. vaccination is not feasible in larvae and antibiotics have reduced efficacy against multidrug resistant bacteria. a novel approach to controlling vibrio infections in aquaculture is needed. the potential of phage therapy to combat vibriosis in fish larvae production has not yet been examined. we describe the isolation and characterization of two ... | 2014 | 25464504 |
| structural characterization and expression analysis of a novel cysteine protease inhibitor from haliotis discus hannai ino. | the sequence of the cysteine protease inhibitor gene of haliotis discus hannai (designated hdcpi) was determined using the race method. the full-length hdcpi cdna is 1049 bp long, and contains an open reading frame of 813 bp, encoding a 271-amino-acid protein with a calculated molecular mass of 29.83 kda and an isoelectric point of 8.57. the deduced amino acid sequence of hdcpi contains two cystatin-like domains, and each has the structural features of the cystatin family, including three evolut ... | 2015 | 25463299 |
| molecular characterization of collagen iv evidences early transcription expression related to the immune response against bacterial infection in the red abalone (haliotis rufescens). | collagen iv has been described as a structural protein of the basement membrane, which as a whole forms a specialized extracellular matrix. recent studies have indicated a possible relationship between collagen iv and the innate immune response of invertebrate organisms. the present study characterized the alpha-1 chain of collagen iv in the red abalone haliotis rufescens (hr-coliv) and evaluated its association with the innate immune response against vibrio anguillarum. to further evidence the ... | 2015 | 25463284 |
| recombinant expression and characterization of a serine protease inhibitor (lvserpin7) from the pacific white shrimp, litopenaeus vannamei. | serine protease inhibitors (serpins) are widely known to its inhibitory role on proteases involved in the immune responses. herein, a novel serine protease inhibitor (lvserpin7), encoding for 411 amino acids with calculated molecular mass of 46.29 kda and isoelectric point of 6.98 was characterized from the pacific white shrimp litopenaeus vannamei. lvserpin7 shared 92.9% identities to penaeus monodon serpin7. among the tested tissues, lvserpin7 was mainly expressed in hemocytes and gill. the ex ... | 2015 | 25462553 |
| lect2 improves the outcomes in ayu with vibrio anguillarum infection via monocytes/macrophages. | leukocyte cell-derived chemotaxin 2 (lect2) is reported to be a cytokine involved in the immune response against pathogenic microorganisms in fish. however, its accurate function in whole fish remains unclear. in this study, we provide the first report on the effect of lect2 on fish defenses against pathogens in vivo. the administration of recombinant lect2 improved the survival rate of vibrio anguillarum infected ayu. the bacterial burden of v. anguillarum infected ayu was decreased in lect2-tr ... | 2014 | 25462453 |
| the tnfα converting enzyme (tace) from ayu (plecoglossus altivelis) exhibits tnfα shedding activity. | tnfα converting enzyme (tace) is responsible for converting membrane-anchored tnfα to its soluble form in mammalian. however, the function and characteristics of tace in teleosts is unclear. in this study, we report the cloning of a cdna sequence of the patace from ayu, plecoglossus altivelis. patace encodes an 865-aa polypeptide, which is closest to the tace gene found in pufferfish (takifugu rubripes). patace mrna was detected in all the tissues tested, although it was considerably higher in l ... | 2015 | 25458310 |
| defensive response of european sea bass (dicentrarchus labrax) against listonella anguillarum or photobacterium damselae subsp. piscicida experimental infection. | sea bass were experimentally infected with listonella anguillarum or photobacterium damselae subsp. piscicida (phdp). at 24 and 72h post-infection, the expression analysis of immune-relevant genes (il-1β, il-6, il-8, hepcidin), the transcriptional level and detection of hsp70, and the quantification of serum iron were investigated in association with the histological analysis and the bacterial recognition in tissues by immunohistochemistry. at 15 days post-infection, the specific antibody respon ... | 2014 | 25454083 |
| effects of recombinant flagellin b and its nd1 domain from vibrio anguillarum on macrophages from gilthead seabream (sparus aurata l.) and rainbow trout (oncorhynchus mykiss, w.). | flagellin is the principal component of flagellum in gram negative and positive bacteria, and it is also the ligand that activates the toll-like receptor 5 (tlr5) in mammals and fish. in higher vertebrates, flagellin induces the activation of the membrane-bound tlr5 (tlr5m), which promotes the expression of proinflammatory cytokines and chemokines and the co-stimulatory molecules present in antigen-presenting cells needed for the activation of t cells. in the present study, we report the product ... | 2015 | 25449380 |
| involvement of a serpin serine protease inhibitor (ooserpin) from mollusc octopus ocellatus in antibacterial response. | serpin is an important member of serine protease inhibitors (spis), which is capable of regulating proteolytic events and involving in a variety of physiological processes. in present study, a serpin homolog was identified from octopus ocellatus (designated as ooserpin). full-length cdna of ooserpin was of 1735 bp, containing a 5' untranslated region of 214 bp, a 3' utr of 282 bp, and an open reading frame of 1239 bp. the open reading frame encoded a polypeptide of 412 amino acids which has a pr ... | 2015 | 25449372 |
| increased survival of juvenile turbot scophthalmus maximus by using bacteria associated with cultured oysters. | preventing vibriosis in juvenile cultured turbot scophthalmus maximus caused by vibrio anguillarum frequently requires the use of feed supplemented with antibiotics in addition to vaccines. whether the use of probiotics instead of antibiotics in juvenile turbot is a safer strategy requires more study. the antibacterial potential of 148 vibrio spp. strains (mostly isolated from cultures of healthy oysters, clams, and turbot) was analyzed in vitro against v. anguillarum and other pathogens by mean ... | 2014 | 25361445 |
| rpos and indole signaling control the virulence of vibrio anguillarum towards gnotobiotic sea bass (dicentrarchus labrax) larvae. | quorum sensing, bacterial cell-to-cell communication with small signal molecules, controls the virulence of many pathogens. in contrast to other vibrios, neither the vani/vanr acylhomoserine lactone quorum sensing system, nor the three-channel quorum sensing system affects virulence of the economically important aquatic pathogen vibrio anguillarum. indole is another molecule that recently gained attention as a putative signal molecule. the data presented in this study indicate that indole signal ... | 2014 | 25360804 |
| sghc1q, a novel c1q family member from half-smooth tongue sole (cynoglossus semilaevis): identification, expression and analysis of antibacterial and antiviral activities. | the c1q family includes many proteins that contain a globular (gc1q) domain, and this family is widely conserved from bacteria to mammals. the family is divided into three subgroups: c1q, c1q-like and ghc1q. in this study, a novel c1q family member, sghc1q, was cloned and identified from cynoglossus semilaevis (named cssghc1q). the full-length cssghc1q cdna spans 905 bp, including an open reading frame (orf) of 768 bp, a 5'-untranslated region (utr) of 25 bp and a 3'-utr of 112 bp. the orf encod ... | 2015 | 25312696 |
| hdhctl1 is a novel c-type lectin of abalone haliotis discus hannai that agglutinates gram-negative bacterial pathogens. | c-type lectins (ctls) are ca(2+)-dependent carbohydrate recognition proteins, which play important roles in the innate immunity of both vertebrates and invertebrates. in this study, we identified and characterized a c-type lectin (named hdhctl1) from pacific abalone, haliotis discus hannai. hdhctl1 is composed of 176 amino acid residues and shares low (23.9%) identity with the known ctl of abalone. hdhctl1 possesses a putative signal peptide and a carbohydrate-recognition domain (crd) typical of ... | 2014 | 25301718 |
| the catecholamine stress hormones norepinephrine and dopamine increase the virulence of pathogenic vibrio anguillarum and vibrio campbellii. | obtaining a better understanding of mechanisms involved in bacterial infections is of paramount importance for the development of novel agents to control disease caused by (antibiotic resistant) pathogens in aquaculture. in this study, we investigated the impact of catecholamine stress hormones on growth and virulence factor production of pathogenic vibrios (i.e. two vibrio campbellii strains and two vibrio anguillarum strains). both norepinephrine and dopamine (at 100 μm) significantly induced ... | 2014 | 25264299 |
| a live attenuated combination vaccine evokes effective immune-mediated protection against edwardsiella tarda and vibrio anguillarum. | edwardsiella tarda and vibrio anguillarum are the two main pathogenic bacteria that cause edwardsiellosis and vibriosis in various species of fish raised in aquaculture. in our previous study, the live attenuated vaccines e. tarda wed and v. anguillarum mvav6203 showed robust relative protection when vaccinated zebrafish or turbot were challenged with virulent e. tarda or v. anguillarum, respectively. additionally, vaccinated fish processed the two vaccines through different pathways of antigen ... | 2014 | 25219565 |
| a new group of anti-lipopolysaccharide factors from marsupenaeus japonicus functions in antibacterial response. | anti-lipopolysaccharide factors (alfs) are a group of critical effector molecules with a broad spectrum of antimicrobial activities in crustaceans. four groups of alfs (a, b, c, and d) have been identified in peneaid shrimp. in the study, we identified a new group of alfs (designated as mjalf-e) from marsupenaeus japonicus. this new group (group e) included mjalf-e1 and e2. mjalf-e1 was highly expressed in hemocytes, heart, and intestine, whereas e2 was highly expressed in gills, stomach, and in ... | 2015 | 25218642 |
| comparative genomic of the teleost cathepsin b and h and involvement in bacterial induced immunity of miiuy croaker. | cathepsins are a family of lysosomal proteases play different roles at physiological and pathological states and present in almost all animals as well as other organisms. cathepsins b and h are both cysteine proteases of cathepsins. cathepsin b and h have been studied playing parts in protein degradation/turnover, antigen presentation/processing and hormone maturation in mammals. however, little is known about the structures and functions of cathepsin b and h in teleosts. in the present study, w ... | 2014 | 25181651 |
| characterization, evolution and functional analysis of the liver-expressed antimicrobial peptide 2 (leap-2) gene in miiuy croaker. | as an evolutionarily older defense strategy, the innate immune is the dominant immune system and provides a first line of antimicrobial host defense in teleost. liver-expressed antimicrobial peptide-2 (leap-2) is a critical molecule of the innate immune system and plays a very important role in resistance against bacterial infections. we reported comprehensive analysis and characterization of leap-2 gene from miiuy croaker (miichthys miiuy) in here. the complete cdna of miiuy croaker leap-2 cons ... | 2014 | 25180825 |
| synthesis and bioactivity of analogues of the marine antibiotic tropodithietic acid. | tropodithietic acid (tda) is a structurally unique sulfur-containing antibiotic from the roseobacter clade bacterium phaeobacter inhibens dsm 17395 and a few other related species. we have synthesised several structural analogues of tda and used them in bioactivity tests against staphylococcus aureus and vibrio anguillarum for a structure-activity relationship (sar) study, revealing that the sulfur-free analogue of tda, tropone-2-carboxylic acid, has an antibiotic activity that is even stronger ... | 2014 | 25161739 |
| host-induced increase of sea bass mortality in a gnotobiotic challenge test with vibrio anguillarum. | 2013 | 25141680 | |
| comparative study of immune responses in the deep-sea hydrothermal vent mussel bathymodiolus azoricus and the shallow-water mussel mytilus galloprovincialis challenged with vibrio bacteria. | the deep-sea hydrothermal vent mussel bathymodiolus azoricus and the continental european coast mytilus galloprovincialis are two bivalves species living in highly distinct marine habitats. mussels are filter-feeding animals that may accumulate rapidly bacteria from the environment. contact with microorganism is thus inevitable during feeding processes where gill tissues assume a strategic importance at the interface between the external milieu and the internal body cavities promoting interactio ... | 2014 | 25089010 |
| comparative genome sequencing to assess the genetic diversity and virulence attributes of 15 vibrio anguillarum isolates. | vibrio anguillarum is the causative agent of vibriosis, a deadly haemorrhagic septicaemic disease affecting various marine and fresh/brackish water fish, bivalves and crustaceans. however, the diversity and virulence mechanisms of this pathogen are still insufficiently known. in this study, we aimed to increase our understanding of v. anguillarum diversity and virulence through comparative genome analysis of 15 v. anguillarum strains, obtained from different hosts or non-host niches and geograph ... | 2015 | 25073650 |
| molecular cloning and characterization of secretory and membrane-bound igm of turbot. | in recent years, increasing diseases especially bacterial diseases have brought a host of losses with the expansive cultivation of turbot (scophthalmus maximus). in order to do more research about the immune system of turbot for better understanding the mechanism of resisting diseases, the immunoglobulin genes related to secretory and membrane-bound igm (s-igm and m-igm) of turbot were cloned using homology sequences cloning and smart race pcr method. the heavy chain of s-igm cdna is 1900 bp in ... | 2014 | 25066925 |
| lipopolysaccharide and β-1,3-glucan binding protein in the hard clam (meretrix meretrix): molecular characterization and expression analysis. | pattern recognition molecules play an important role in innate immunity by recognizing conserved molecular patterns that are present on the surface of invading microorganisms. in this study, a lipopolysaccharide and β-1,3-glucan binding protein (lgbp) gene was cloned from the hard clam meretrix meretrix (designated as mm-lgbp) by the expressed sequence tags and rapid amplification of cdna ends method. the cdna was 1827 bp in length, consisting of a 71-bp 5'-terminal untranslated region, a 62-bp ... | 2014 | 25062483 |
| development of a two-step, non-probed multiplex real-time pcr for surveilling vibrio anguillarum in seawater. | vibrio anguillarum is an aggressive and halophilic bacterial pathogen most commonly originating from seawater. vibrio anguillarum presence in fisheries and aquaculture facilities causes significant morbidity and mortality among aquaculture species primarily from haemorrhaging of the body and skin of the infected fish that eventually leads to death, collectively recognized as the disease vibriosis. this study served to develop a non-probe, multiplex real-time pcr assay to rapidly detect v. anguil ... | 2015 | 25039502 |
| characterization and expression pattern of a novel β-defensin in common carp (cyprinus carpio l.): implications for its role in mucosal immunity. | β-defensins are a group of cysteine-rich cationic antimicrobial peptides that play antibacterial and antiviral roles in immune systems of vertebrates. here, we report the cloning and identification of a β-defensin 3 cdna sequence from the common carp (cyprinus carpio l.). sequence alignment and phylogenetic analysis indicated that this β-defensin 3 belonged to the bd-2 group of fish. real-time pcr showed that the β-defensin 3 mrna was expressed in all the tissues of normal common carp that we ex ... | 2014 | 25036829 |
| iron shortage and bile salts play a major role in the expression of ompk gene in vibrio anguillarum. | the outer membrane protein k, ompk first identified in vibrio parahaemolyticus has been shown to be a receptor for a broad host range vibriophage kvp40 infecting members of the vibrionaceae. in the study, the effect of culture conditions on the expression of ompk in v. anguillarum was studied using real-time pcr. the expression increased significantly in the presence of bile salts and iron chelating agent 2, 2' bipyridine, suggesting a role for this protein in bile resistance and also in iron ac ... | 2014 | 25033659 |
| characterization and expression of sweetfish (pleco glossus altivelis) cathepsin d. | cathepsin d (ctsd) is a lysosomal acidic endoproteinase that plays an important role in immune response. in this study, we obtained sweetfish (plecoglossus altivelis) ctsd (pactsd) via de-novo transcriptome sequencing of sweetfish macrophages. the full length cdna sequence of pactsd was 1 955 bp encoding a propeptide of 397 amino acids. the deduced protein had a calculated molecular weight of 43.17 × 10³. multiple alignment with other known ctsd amino acid sequences revealed amino acid conservat ... | 2014 | 25017748 |
| complete genome sequence of vibrio anguillarum phage choed successfully used for phage therapy in aquaculture. | vibrio anguillarum phage choed was isolated from chilean mussels. it is a virulent phage showing effective inhibition of v. anguillarum. choed has potential in phage therapy, because it can protect fish from vibriosis in fish farms. here, we announce the completely sequenced genome of v. anguillarum phage choed. | 2014 | 25013148 |
| alternative splicing and immune response of crassostrea gigas tumor necrosis factor receptor-associated factor 3. | diverse alternative splicing isoforms play an important role in immune diversity and specificity. their role in molluscan host-defense is however poorly understood. we characterized two alternative isoforms of tumor necrosis factor receptor-associated factor 3 (traf3) in the pacific oyster, crassostrea gigas, which were named cgtraf3-s and cgtraf3-l. an intron was retained in cgtraf3-l, introducing a premature termination codon. comparison and phylogenetic analysis revealed that cgtraf3 shared a ... | 2014 | 25012913 |
| molecular characterization of a cxcl8-like protein from ayu and its effect on chemotaxis of neutrophils and monocytes/macrophages. | cxcl8, a cxc-type chemokine, plays a crucial role in acute inflammation by recruiting and mediating neutrophils and other cells. in this study, the cdna and genomic dna sequence of a cxcl8-like protein (pacxcl8l) from ayu (plecoglossus altivelis) was determined. sequence analysis showed that pacxcl8l represented the typical structure of animal cxcl8s. phylogenetic tree analysis indicated that pacxcl8l was closest to cxcl8 of atlantic cod (gadus morhua). constitutive expression of pacxcl8l was de ... | 2014 | 25010728 |
| atp release channel pannexin1 is a novel immune response gene in japanese flounder paralichthys olivaceus. | extracellular atp is an important damage-associated molecular pattern molecule that plays key roles in innate immunity. in fish, however, the mechanism for extracellular atp release remains largely undefined. pannexin1 (panx1) is a newly discovered extracellular atp release channel with a wide tissue distribution and diverse biological functions in mammals. in the present study, we identified and characterized a panx1 homolog cdna, termed popanx1, from japanese flounder paralichthys olivaceus, w ... | 2014 | 25007779 |
| calmodulin is a stress and immune response gene in chinese mitten crab eriocheir sinensis. | calmodulin (cam) is a multifunctional calcium sensor protein that participates in various cellular processes under normal, stress and pathological conditions. in crabs, however, the involvement of cam in response to environmental stress and immune challenges has not been revealed yet. in the present study, a cam cdna (escam) was identified from chinese mitten crab eriocheir sinensis and its mrna expression patterns in response to ambient (salinity and ph) stress and immune challenges was examine ... | 2014 | 24997436 |
| notable mucosal immune responses induced in the intestine of zebrafish (danio rerio) bath-vaccinated with a live attenuated vibrio anguillarum vaccine. | live attenuated vaccine is one of the efficient vaccine candidates in aquaculture, which can be easily delivered to fish via bath-vaccination. an outstanding advantage of bath-vaccination is that vaccine delivery is through the same route as that utilized by many fish pathogens, generating specific mucosal immune responses. in this work, we investigated the mucosal immune responses induced by a live attenuated vibrio anguillarum vaccine in zebrafish via bath-vaccination. bacteria proliferated ra ... | 2014 | 24997435 |
| three representative subtypes of caspase in miiuy croaker: genomic organization, evolution and immune responses to bacterial challenge. | caspase proteins are intracellular proteases which function as initiators and effectors of apoptosis. according their difference of functions, the caspases can be divided into apoptosis related caspases and inflammatory mediator, and the former included apoptosis activator and apoptosis executioner. in this study, three different subtype caspases (caspase1, caspase3 and caspase9) from miichthys miiuy miiuy croaker were analyzed. the caspase1 belongs to the inflammatory mediator, caspase3 belongs ... | 2014 | 24973513 |
| lipopolysaccharides isolated from aeromonas salmonicida and vibrio anguillarum show quantitative but not qualitative differences in inflammatory outcome in sparus aurata (gilthead seabream). | in fish, the defence system recognises pathogenic microorganisms via pathogen recognition receptors (prrs) that sense particular structures of the pathogens; the so-called pathogen associated molecular patterns (pamps) such as bacterial lipopolysaccharides (lpss). the result of the pamp-prr interactions leads to complex and orchestrated immune responses. in this study, sparus aurata (gilthead seabream) were intraperitoneally injected with purified lipopolysaccharide (lps) from aeromonas salmonic ... | 2014 | 24954838 |
| development and evaluation of a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip lamp) for rapid and simultaneous detection of ten pathogenic bacteria in aquatic animals. | rapid, low-cost, and user-friendly strategies are urgently needed for early disease diagnosis and timely treatment, particularly for on-site screening of pathogens in aquaculture. in this study, we successfully developed a real-time fluorogenic loop-mediated isothermal amplification assay integrated on a microfluidic disc chip (on-chip lamp), which was capable of simultaneously detecting 10 pathogenic bacteria in aquatic animals, i.e., nocardia seriolae, pseudomonas putida, streptococcus iniae, ... | 2014 | 24954661 |
| a galectin with quadruple-domain from red abalone haliotis rufescens involved in the immune innate response against to vibrio anguillarum. | galectins are proteins that recognize and bind specifically β-galactosidase residues, playing important roles in the innate immune response of vertebrates and invertebrates. the cdna of a tandem repeat galectin from the red abalone haliotis rufescens cdna (hrgal) was cloned and characterized using rapid amplification of cdna end technique. the full-length cdna of hrgal was 2471 bp, with a 5' terminal untranslated region (utr) of 131 bp, a 3' utr of 672 pb, and an open reading frame (orf) of 1668 ... | 2014 | 24952088 |
| expression, purification and characterization of soluble recombinant peptidyl-prolyl cis/trans isomerase from vibrio anguillarum. | vibrio anguillarum, a causative agent of vibriosis in finfish, crustaceans, and bivalves, is a gram-negative, motile marine bacterium. most bacteria have developed survival strategies in various environments. the aim of this study was to investigate the changes in protein expression of v. anguillarum o1 incubated under different conditions using two dimensional electrophoresis and maldi-tof ms/ms analysis. result indicated that peptidyl-prolyl cis/trans isomerase (ppiase) expression was increasi ... | 2014 | 24931498 |
| assessment of planctomycetes cell viability after pollutants exposure. | in this study, the growth of six different planctomycetes, a particular ubiquitous bacterial phylum, was assessed after exposure to pollutants. in addition and for comparative purposes, pseudomonas putida, escherichia coli and vibrio anguillarum were tested. each microorganism was exposed to several concentrations of 21 different pollutants. after exposure, bacteria were cultivated using the drop plate method. in general, the strains exhibited a great variation of sensitivity to pollutants in th ... | 2014 | 24903954 |
| first total syntheses and antimicrobial evaluation of penicimonoterpene, a marine-derived monoterpenoid, and its various derivatives. | the first total synthesis of marine-derived penicimonoterpene (±)-1 has been achieved in four steps from 6-methylhept-5-en-2-one using a reformatsky reaction as the key step to construct the basic carbon skeleton. a total of 24 new derivatives of 1 have also been designed and synthesized. their structures were characterized by analysis of their 1h nmr, 13c nmr and hresims data. some of them showed significant antibacterial activity against aeromonas hydrophila, escherichia coli, micrococcus lute ... | 2014 | 24897384 |
| characterization and function analysis of an anti-lipopolysaccharide factor (alf) from the chinese shrimp fenneropenaeus chinensis. | anti-lipopolysaccharide factor (alf) is one of the widely-studied antimicrobial peptides (amps) with broad-spectrum antibacterial activity and antiviral property. previous studies show the existence of multiform of alfs in crustacean which are important for immunity of the animals. in the present study, we characterized one isoform of alf from the chinese shrimp fenneropenaeus chinensis (fcalf2). tissue distribution analysis revealed that fcalf2 showed the highest expression level in the lymphoi ... | 2014 | 24879940 |
| calnexin functions in antibacterial immunity of marsupenaeus japonicus. | calnexin (cnx) is an endoplasmic reticulum membrane-bound lectin chaperone that comprises a dedicated maturation system with another lectin chaperone calreticulin (crt). this maturation system is known as the cnx/crt cycle. the main functions of cnx are ca(2+) storage, glycoprotein folding, and quality control of synthesis. recent studies have shown that cnx is important in phagocytosis and in optimizing dendritic cell immunity. however, the functions of cnx in invertebrate innate immunity remai ... | 2014 | 24858031 |
| dietary supplementation of pediococcus pentosaceus enhances innate immunity, physiological health and resistance to vibrio anguillarum in orange-spotted grouper (epinephelus coioides). | groupers (epinephelus spp.) are economically important fish species in southeast asian aquaculture. vibriosis caused by vibro spp. is one of the severe bacterial diseases that devastate the grouper aquaculture industry. probiotics have been reported to show the potential to enhance fish immunity and to antagonize pathogens. in our previous study, a lactic acid bacterium pediococcus pentosaceus strain 4012 (lab4012), isolated from cobia intestine, protects cobia from photobacteriosis after a 2-we ... | 2014 | 24845519 |
| molecular characteristic and expression analysis of collagenolytic serine protease from the chinese mitten crab eriocheir sinensis with defense response to vibrio anguillarum challenge. | a novel collagenolytic serine protease (clsp) was cloned from the hemocytes of the chinese mitten crab eriocheir sinensis (es-clsp). the full-length cdna of es-clsp contains 990 nucleotides. it encodes a 270-amino acid-long peptide with the mature peptide containing 221 amino acids. it contains the conserved catalytic triad (h, d, and s). similarity analysis shows that es-clsp shares high identity with clsps from the fiddler crab uca pugilator. es-clsp mrna expression in e. sinensis is a) tissue ... | 2014 | 24841909 |
| a fibrinogen-related protein (frep) is involved in the antibacterial immunity of marsupenaeus japonicus. | fibrinogen-related proteins (freps) in invertebrates have important functions in innate immunity. in this study, the cdna of frep was identified from the kuruma shrimp marsupenaeus japonicus (mjfrep2). the full-length cdna of mjfrep2 is 1138 bp with an open reading frame of 954 bp that encodes a 317-amino acid protein comprising a signal peptide and a fibrinogen-like domain. mjfrep2 could be detected in hemocytes, heart, hepatopancreas, gills, stomach, and intestines. mjfrep2 could also be upreg ... | 2014 | 24830772 |
| immune response of apoptosis-related cysteine peptidases from the red abalone haliotis rufescens (hrcas8 and hrcas3): molecular characterization and transcription expression. | caspases play an important role in the different stages of programmed cell death, or apoptosis, which has been related to the immune response in multicellular organisms. the present study characterized an initiator caspase (hrcas8) and an effector caspase (hrcas3) from the red abalone haliotis rufescens using the race method and qpcr analysis. hrcas8 showed a complete sequence of 2529 base pairs (bp) with an open-reading frame (orf) of 1911 bp, a 5'utr of 201 bp, and a 3'utr of 417 bp. the estim ... | 2014 | 24821426 |
| role of evolutionarily conserved signaling intermediate in toll pathways (ecsit) in the antibacterial immunity of marsupenaeus japonicus. | the toll/toll-like receptor (tlr) signaling pathway has an important role in the innate immunity of animals. evolutionarily conserved signaling intermediate in toll pathways (ecsit) is a protein that functions as an adaptor protein for the toll/tlr and bone morphogenetic protein signaling pathways. ecsit is also a key component in the macrophage bactericidal activity of mammals. however, the function of ecsit in crustaceans remains unclear. in this study, we cloned and identified a functional ec ... | 2014 | 24796866 |
| molecular characterization and expression analysis of atp-gated p2x7 receptor involved in japanese flounder (paralichthys olivaceus) innate immune response. | atp-gated p2x7 receptor (p2rx7) channel is a key component for purinergic signaling and plays important roles in the innate immune response in mammals. however, the expression, molecular properties and immune significances of p2rx7 in lower vertebrates are still very limited. here we identified and characterized a novel bony fish p2rx7 homologue cdna, termed pop2rx7, in japanese flounder (paralichthys olivaceus). pop2rx7 protein shares about 60-88% sequence similarity and 45-78% sequence identit ... | 2014 | 24796752 |
| identification, expression, and responses to bacterial challenge of the cathepsin c gene from the razor clam sinonovacula constricta. | cathepsin c (dipeptidyl-peptidasei, dppi) is a lysosomal cysteine proteinase that belongs to the papain superfamily, and it is involved in protein degradation and proenzyme activation. however, very little is known about the function of cathepsin c in bivalves. in the present study, we identified the cathepsin c gene in the razor clam sinonovacula constricta (sc-ctsc). the full-length sc-ctsc cdna contained a complete open reading frame (orf) of 1371 nt encoding 456 amino acids, a 98 bp 5' utr, ... | 2014 | 24792213 |