Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| an assessment of bacterial dysbiosis in pouchitis using terminal restriction fragment length polymorphisms of 16s ribosomal dna from pouch effluent microbiota. | previous studies on dysbiosis and pouchitis using conventional culture techniques have been disappointing because of inherent limitations associated with the technique. this study was designed to use terminal restriction fragment length polymorphism to evaluate patients with and without pouchitis. | 2009 | 19617766 |
| evaluation of two spike-and-recovery controls for assessment of extraction efficiency in microbial source tracking studies. | quantitative pcr (qpcr), applied to complex environmental samples such as water, wastewater, and feces, is susceptible to methodological and sample related biases. in this study, we evaluated two exogenous dna spike-and-recovery controls as proxies for recovery efficiency of bacteroidales 16s rdna gene sequences (allbac and qhf183) that are used for microbial source tracking (mst) in river water. two controls--(1) the plant pathogen pantoea stewartii, carrying the chromosomal target gene cpsd, a ... | 2009 | 19589555 |
| a novel inhalation challenge set to study animal model of allergic alveolitis. | a novel inhalation challenge set for the study of experimental allergic alveolitis (hypersensitivity pneumonitis) in mice was designed. a finely dispersed aerosol of allergenic extract generated by the commercial ultrasonic nebulizer "tajfun mu1" (produced by medbryt, warsaw, poland) was transported to the airtight inhalation chamber. in the chamber were placed 15 perforated containers made of transparent plastic, each containing one mouse. they were coupled in 3 units, each consisted of 5 conta ... | 2009 | 19572490 |
| occupational exposure to organic dust, microorganisms, endotoxin and peptidoglycan among plants processing workers in poland. | the objective of present work was to determine and compare the components of bioaerosol in several sectors of plant processing industries. the study was conducted in 10 facilities engaged in herb and grain processing, flax threshing, grain storing, baking, and cereals production. the air samples were taken on glass fibre filters with an as-50 sampler. we determined the concentrations of airborne microorganisms, dust, endotoxin and peptidoglycan. total concentrations of viable airborne microorgan ... | 2009 | 19572487 |
| broad-range pcr, cloning and sequencing of the full 16s rrna gene for detection of bacterial dna in synovial fluid samples of tunisian patients with reactive and undifferentiated arthritis. | broad-range rdna pcr provides an alternative, cultivation-independent approach for identifying bacterial dna in reactive and other form of arthritis. the aim of this study was to use broad-range rdna pcr targeting the 16s rrna gene in patients with reactive and other forms of arthritis and to screen for the presence of dna from any given bacterial species in synovial fluid (sf) samples. | 2009 | 19570210 |
| carotenoids' influence on radiotolerance of pantoea agglomerans, a plant pathogen. | the aim of this study was to evaluate the effect of gamma radiation on the carotenoid content of two strains of the enterobacteriaceae: pantoea agglomerans. | 2008 | 19552787 |
| arsenic detoxification potential of aox genes in arsenite-oxidizing bacteria isolated from natural and constructed wetlands in the republic of korea. | arsenic is subject to microbial interactions, which support a wide range of biogeochemical transformations of elements in natural environments such as wetlands. the arsenic detoxification potential of the bacterial strains was investigated with the arsenite oxidation gene, aox genotype, which were isolated from the natural and constructed wetlands. the isolates were able to grow in the presence of 10 mm of sodium arsenite (as(iii) as naaso(2)) and 1 mm of d: +glucose. phylogenetic analysis based ... | 2010 | 19548094 |
| bacterial community and nitrogen fixation in the red turpentine beetle, dendroctonus valens leconte (coleoptera: curculionidae: scolytinae). | the red turpentine beetle, dendroctonus valens leconte (coleoptera: curculionidae: scolytinae), colonizes all pines species within its native range throughout north and central america. recently, this species was accidentally introduced to china, where it has caused severe damage in pine forests. it belongs to a group of beetles that spend most of their lives between the tree bark and sapwood, where it feeds on phloem: a poor substrate with very low nutritional value of nitrogen and toxic proper ... | 2009 | 19543937 |
| regulatory interactions between quorum-sensing, auxin, cytokinin, and the hrp regulon in relation to gall formation and epiphytic fitness of pantoea agglomerans pv. gypsophilae. | gall formation by pantoea agglomerans pv. gypsophilae is controlled by hrp/hrc genes, phytohormones, and the quorum-sensing (qs) regulatory system. the interactions between these three components were investigated. disruption of the qs genes pagi and pagr and deletion of both substantially reduced the transcription levels of the hrp regulatory genes hrpxy, hrps, and hrpl, as determined by quantitative reverse-transcriptase polymerase chain reaction. expression of hrpl in planta was inhibited by ... | 2009 | 19522567 |
| the microbiota of lafun, an african traditional cassava food product. | lafun is a fermented cassava food product consumed in parts of west africa. in the present work the microorganisms (aerobic bacteria (ab), lactic acid bacteria (lab) and yeasts) associated with the fermentation of lafun under traditional conditions have for the first time been studied using a combination of pheno- and genotypic methods. during lafun fermentation the ab count ranged from 6-7 log(10) cfu/g at the beginning to 9 log(10) cfu/g at the end. similarly, the number of lab increased from ... | 2009 | 19493582 |
| phenotypic and genetic diversity of rice seed-associated bacteria and their role in pathogenicity and biological control. | to study the phenotypic and genetic diversity of culturable bacteria associated with rice seed and to asses the antagonistic and pathogenic potential of the isolated bacteria. | 2009 | 19486410 |
| comparative genomics-guided loop-mediated isothermal amplification for characterization of pseudomonas syringae pv. phaseolicola. | to design and evaluate a loop-mediated isothermal amplification (lamp) protocol by combining comparative genomics and bioinformatics for characterization of pseudomonas syringae pv. phaseolicola (psp), the causal agent of halo blight disease of bean (phaseolus vulgaris l.). | 2009 | 19486391 |
| antibiotic resistance of gram negatives isolates from loggerhead sea turtles (caretta caretta) in the central mediterranean sea. | previous studies on fish and marine mammals support the hypothesis that marine species harbor antibiotic resistance and therefore may serve as reservoirs for antibiotic-resistance genetic determinants. the aim of this study was to assess the resistance to antimicrobial agents of gram negative strains isolated from loggerhead sea turtles (carettacaretta). oral and cloacal swabs from 19 live-stranded loggerhead sea turtles, with hooks fixed into the gut, were analyzed. the antimicrobial resistance ... | 2009 | 19473669 |
| isolation and characterization of rhamnolipid-producing bacterial strains from a biodiesel facility. | novel strains of rhamnolipid-producing bacteria were isolated from soils at a biodiesel facility on the basis of their ability to grow on glycerol as a sole carbon source. strains were identified as acinetobacter calcoaceticus, enterobacter asburiae, enterobacter hormaechei, pantoea stewartii, and pseudomonas aeruginosa. the strains of the former five species were found to produce rhamnolipids in quantities the same as, or similar to, coisolated strains of p. aeruginosa. measurements of surface ... | 2009 | 19473254 |
| bacterial associates of arboreal ants and their putative functions in an obligate ant-plant mutualism. | bacterial communities are highly diverse and have great ecological importance. in the present study, we used an in silico analysis of terminal restriction fragments (trf) to characterize the bacterial community of the plant ant pseudomyrmex ferrugineus. this species is an obligate inhabitant of acacia myrmecophytes and feeds exclusively on plant-derived food sources. ants are the dominant insect group in tropical rain forests. associations of ants with microbes, which contribute particularly to ... | 2009 | 19447959 |
| multiple activities of the plant pathogen type iii effector proteins wtse and avre require wxxxe motifs. | the broadly conserved avre-family of type iii effectors from gram-negative plant-pathogenic bacteria includes important virulence factors, yet little is known about the mechanisms by which these effectors function inside plant cells to promote disease. we have identified two conserved motifs in avre-family effectors: a wxxxe motif and a putative c-terminal endoplasmic reticulum membrane retention/retrieval signal (ermrs). the wxxxe and ermrs motifs are both required for the virulence activities ... | 2009 | 19445595 |
| blood culture status in mature horses with diarrhoea: a possible association with survival. | the incidence and implications of positive blood cultures in mature horses with diarrhoea is unknown. the diagnosis of bacteraemia may alter treatment and prognosis. | 2009 | 19418745 |
| utility and safety of lps-based fermented flour extract as a macrophage activator. | the immune system is part of the homeostasis system. our research is focused on ways to maintain health, with an emphasis on the role of macrophages. we have hypothesized that tissue macrophages form a systemic network which we believe contributes to the homeostasis system, and have named it the 'macrophage network.' this network creates a dynamic equilibrium situation where macrophages control homeostasis. our research is based on this macrophage network theory as we believe that the innate imm ... | 2009 | 19414320 |
| isolation and characterization of plant growth-promoting strain pantoea nii-186. from western ghat forest soil, india. | to isolate plant growth-promoting bacterium from western ghat forests in india. | 2009 | 19413772 |
| pantoea ananatis: an unconventional plant pathogen. | pantoea ananatis causes disease symptoms in a wide range of economically important agricultural crops and forest tree species worldwide. it is regarded as an emerging pathogen based on the increasing number of reports of diseases occurring on previously unrecorded hosts in different parts of the world. its unconventional nature lies in the fact that, unlike the majority of plant pathogenic microbes, p. ananatis is capable of infecting humans and occurs in diverse ecological niches, such as part ... | 2009 | 19400836 |
| recent evolution of bacterial pathogens: the gall-forming pantoea agglomerans case. | pantoea agglomerans, a widespread epiphyte and commensal bacterium, has evolved into an hrp-dependent and host-specific tumorigenic pathogen by acquiring a plasmid containing a pathogenicity island (pai). the pai was evolved on an iteron plasmid of the incn family, which is distributed among genetically diverse populations of p. agglomerans. the structure of the pai supports the premise of a recently evolved pathogen. this review offers insight into a unique model for emergence of new bacterial ... | 2009 | 19400643 |
| use of the lambda red-recombineering method for genetic engineering of pantoea ananatis. | pantoea ananatis, a member of the enterobacteriacea family, is a new and promising subject for biotechnological research. over recent years, impressive progress in its application to l-glutamate production has been achieved. nevertheless, genetic and biotechnological studies of pantoea ananatis have been impeded because of the absence of genetic tools for rapid construction of direct mutations in this bacterium. the lambda red-recombineering technique previously developed in e. coli and used for ... | 2009 | 19389224 |
| six cases of sepsis caused by pantoea agglomerans in a teaching hospital. | pantoea agglomerans is an environmental organism which may seldom cause opportunistic infections. here we report on a 6 case outbreak in a teaching hospital. within three months . agglomerans was isolated from blood cultures of 5 patients from oncology and 1 patient from icu departments. p. agglomerans was in pure culture in 5 cases, while in the last one rahnella aquatilis and candida famata were also isolated. therefore, p. agglomerans is able to produce nosocomial infections in patients with ... | 2009 | 19382678 |
| traumatic enucleation with avulsion of the ophthalmic artery resulting in aneurysm-like subarachnoid hemorrhage. | traumatic, nonaneurysmal subarachnoid hemorrhage (sah) is common after closed head injury and most often results from ruptured cortical microvessels. here, the authors present the case of a 60-year-old woman who fell and struck her head, causing traumatic enucleation and avulsion of both the optic nerve and ophthalmic artery. the arterial avulsion caused a fisher grade 3 sah. during her stay in the intensive care unit, hydrocephalus and vasospasm developed, clinical conditions commonly observed ... | 2009 | 19374504 |
| epiphytic bacteria and yeasts on apple blossoms and their potential as antagonists of erwinia amylovora. | apple blossoms were sampled for indigenous epiphytic populations of culturable microorganisms during different stages of bloom at two locations in central washington state and one site in corvallis, or. frequencies and population sizes of bacteria on stigmas of apple were lower in washington than at corvallis, where average relative humidity was higher and possibly favored greater colonization; however, bacteria at corvallis were mainly pseudomonads, whereas those in washington were diverse, com ... | 2009 | 19351253 |
| adverse effects of pantoea peritonitis on peritoneal transport. | 2009 | 19293363 | |
| effect of mutagenesis at the region upstream from the g(q/e) motif of three types of geranylgeranyl diphosphate synthase on product chain-length. | (all-e) geranylgeranyl diphosphate synthases have been classified into three types based on the characteristic sequences around the first aspartate rich motif, which is highly conserved among the enzymes. in type i geranylgeranyl diphosphate synthases, which consist of archaeal enzymes, a bulky amino acid residue at the 5th position upstream from the motif plays a main role in the product determination, by blocking further elongation of prenyl chain as the bottom of the reaction cavity. on the o ... | 2009 | 19269584 |
| temporal analysis of cotton boll symptoms resulting from southern green stink bug feeding and transmission of a bacterial pathogen. | the southern green stink bug, nezara viridula (l.), is a significant pest of cotton, gossypium hirsutum l., and is becoming an increasing challenge due to the decrease in use of broad-spectrum insecticides on the crop. the southern green stink bug can vector an opportunistic pantoea agglomerans strain (designated sc 1-r) into cotton bolls, resulting in infection. the appearance of stink bug damage varies, and pest managers cannot readily identify its source. this research reports a systematic de ... | 2009 | 19253615 |
| study of microbial diversity in raw milk and fresh curd used for fontina cheese production by culture-independent methods. | the bacterial populations of raw milk employed for the production of fontina cheese in alpine farms located in different valleys and altitudes (from 700 to 2246 m above sea level) were investigated by culture independent techniques. total microbial dna was isolated from milk and curd samples and used as template in polymerase chain reaction (pcr) to study the hypervariable v3 region of the bacterial 16s rrna gene and analyzed by denaturing gradient gel electrophoresis (dgge). representative band ... | 2009 | 19232767 |
| inoculation of sugarcane with pantoea sp. increases amino acid contents in shoot tissues; serine, alanine, glutamine and asparagine permit concomitantly ammonium excretion and nitrogenase activity of the bacterium. | pantoea sp. is an endophytic nitrogen-fixing bacterium isolated from sugarcane tissues. the aim of the present study was to determine the contents of amino acids in sugarcane as a result of inoculation of nodes and nodal roots with pantoea sp. strain 9c and to evaluate the effects of amino acids on growth, nitrogenase activity and ammonium excretion of the bacterium. content of almost all amino acids increased in 30-day-old plantlets by root inoculation. the most abundant amino acids in shoot ti ... | 2009 | 19215998 |
| pathway engineering of brassica napus seeds using multiple key enzyme genes involved in ketocarotenoid formation. | brassica napus (canola) plants were genetically manipulated to increase the amount and composition of carotenoids in seeds by using seven key enzyme genes involved in ketocarotenoid formation, which originated from a soil bacterium pantoea ananatis (formerly called erwinia uredovora 20d3), and marine bacteria brevundimonas sp. strain sd212 and paracoccus sp. strain n81106 (formerly called agrobacterium aurantiacum). the seven key gene cassettes, in which each gene was surrounded by an appropriat ... | 2009 | 19204032 |
| effect of gamma-irradiation on membrane fatty acids and peptidoglycan's muropeptides of pantoea agglomerans, a plant pathogen. | the aim of this study was to evaluate the effect of gamma-irradiation on the fatty acids (fa) and muropeptides content of two strains of an enterobacteriacea: pantoea agglomerans. | 2009 | 19191968 |
| diverse antibacterial activity of pectobacterium carotovorum subsp.carotovorum isolated in korea. | fifty-four pectobacterium carotovorum subsp. carotovorum strains isolated in korea were characterized by a spectrum of antibacterial activities against 7 indicator strains chosen to represent various regions and host plants. all p. carotovorum subsp. carotovorum isolates tested could be grouped into 4 classes depending on the pattern of antibacterial substance production. all tested strains had dna fragment(s) homologous to the genes encoding carotovoricin and 21 of them had genes homologous to ... | 2009 | 19190407 |
| lines of evidence for horizontal gene transfer of a phenazine producing operon into multiple bacterial species. | phenazines are secondary metabolites with broad-spectrum antibiotic activity against bacteria, fungi, and eukaryotes. in pseudomonad species, a conserved seven-gene phenazine operon (phzabcdefg) is required for the conversion of chorismic acid to the broad-spectrum antibiotic phenazine-1-carboxylate. previous analyses of genes involved in phenazine production from nonpseudomonad species uncovered a high degree of sequence similarity to pseudomonad homologues. the analyses undertaken in this stud ... | 2009 | 19189039 |
| complete genome of the broad-host-range erwinia amylovora phage phiea21-4 and its relationship to salmonella phage felix o1. | the first complete genome sequence for a myoviridal bacteriophage, phiea21-4, infecting erwinia amylovora, erwinia pyrifoliae, and pantoea agglomerans strains has been determined. the unique sequence of this terminally redundant, circularly permuted genome is 84,576 bp. the phiea21-4 genome has a gc content of 43.8% and contains 117 putative protein-coding genes and 26 trna genes. phiea21-4 is the first phage in which a precisely conserved rho-independent terminator has been found dispersed thro ... | 2009 | 19181832 |
| dna analysis of a radiotolerant bacterium pantoea agglomerans by ft-ir spectroscopy. | a radiation tolerance strain, pantoea agglomerans was isolated from gamma-irradiated carrot samples (daucus carota). d(10) determination showed that the radioresistance of this bacterium is five-fold higher than escherichia coli, both belonging to the family of enterobacteriaceae. dna isolated from untreated and irradiated bacterial cells was analyzed by ft-ir spectroscopy to investigate the radiotolerance of this bacterium. at doses <5kgy, an alteration of the interbase hydrogen networks was ob ... | 2007 | 19071550 |
| characterization of the mineral phosphate-solubilizing activity of pantoea agglomerans mmb051 isolated from an iron-rich soil in southeastern venezuela (bolívar state). | the mineral phosphate-solubilizing (mps) activity of a pantoea agglomerans strain, namely mmb051, isolated from an iron-rich, acidic soil near ciudad piar (bolívar state, venezuela), was characterized on a chemically defined medium (nbrip). various insoluble inorganic phosphates, including tri-calcium phosphate [ca(3)(po(4))(2)], iron phosphate (fepo(4)), aluminum phosphate (alpo(4)), and rock phosphate (rp) were tested as sole sources of p for bacterial growth. solubilization of ca(3)(po(4))(2) ... | 2009 | 19067045 |
| study on the bacterial midgut microbiota associated to different brazilian populations of lutzomyia longipalpis (lutz & neiva) (diptera: psychodidae). | the bacterial community associated with the midgut of three brazilian lutzomyia longipalpis (lutz & neiva) populations, two from endemic areas for visceral leishmaniasis (jacobina, bahia state and são luís, maranhão state) and one from a non-endemic area (lapinha cave, minas gerais state), was identified. five groups, 35 females each, from each population were separated; a total of 175 females per collecting area were analyzed. the species identification was based on molecular and traditional ba ... | 2008 | 19061048 |
| antimicrobial activity of pyrrocidines from acremonium zeae against endophytes and pathogens of maize. | acremonium zeae produces pyrrocidines a and b, which are polyketide-amino acid-derived antibiotics, and is recognized as a seedborne protective endophyte of maize which augments host defenses against microbial pathogens causing seedling blights and stalk rots. pyrrocidine a displayed significant in vitro activity against aspergillus flavus and fusarium verticillioides in assays performed using conidia as inoculum, with pyrrocidine a being more active than b. in equivalent assays performed with c ... | 2009 | 19055442 |
| phylogeny and identification of pantoea species and typing of pantoea agglomerans strains by multilocus gene sequencing. | pantoea agglomerans and other pantoea species cause infections in humans and are also pathogenic to plants, but the diversity of pantoea strains and their possible association with hosts and disease remain poorly known, and identification of pantoea species is difficult. we characterized 36 pantoea strains, including 28 strains of diverse origins initially identified as p. agglomerans, by multilocus gene sequencing based on six protein-coding genes, by biochemical tests, and by antimicrobial sus ... | 2009 | 19052179 |
| wtse, an avre-family type iii effector protein of pantoea stewartii subsp. stewartii, causes cell death in non-host plants. | pantoea stewartii subsp. stewartii (pnss) causes stewart's bacterial wilt of sweet corn and leaf blight of maize. the pathogenicity of pnss depends on synthesis of extracellular polysaccharide and an hrp type iii secretion system. wtse, a type iii secreted effector protein, is essential for the virulence of pnss on corn. it belongs to the avre family of effectors, which includes dspa/e from erwinia amylovora and avre1 from pseudomonas syringae. previously, wtse was shown to cause disease-associa ... | 2008 | 19018993 |
| phylogeny and identification of pantoea species associated with plants, humans and the natural environment based on multilocus sequence analysis (mlsa). | species belonging to the genus of pantoea are commonly isolated from plants, humans and the natural environment. the species of the genus are phenotypically closely related, making rapid identification of pantoea strains to the species level difficult. multilocus sequence analysis (mlsa) was evaluated as a means for rapid classification and identification of pantoea strains. four housekeeping genes, gyrb, rpob, atpd and infb, were sequenced for strains assigned to the genus. included in the stud ... | 2008 | 19008066 |
| isolation of an operon involved in xylitol metabolism from a xylitol-utilizing pantoea ananatis mutant. | an operon involved in cryptic xylitol metabolism of pantoea ananatis was cloned by transposon tagging. a xylitol negative mutant with a transposon insertion in the xylitol 4-dehydrogenase gene (xdh) was isolated and genomic dna around the transposon was sequenced. consequently, six consecutive genes, xytb-g are located downstream of xdh in the same strand. these seven genes are cotranscribed as a single transcript in a p. ananatis xylitol-utilizing mutant, suggesting that they comprise an operon ... | 2008 | 19000608 |
| evaluation of sample preparation methods for the isolation of salmonella from alfalfa and mung bean seeds with the bacteriological analytical manual's salmonella culture method. | five pre-enrichment methods were evaluated for effectiveness with the u.s. food and drug administration's bacteriological analytical manual salmonella culture method in recovering s. stanley, s. poona, and s. muenchen from artificially contaminated alfalfa seeds, and s. saintpaul, s. anatum, and s. infantis from artificially contaminated mung bean seeds. the methods included: (1) soak.--test portions were inoculated into pre-enrichment media; (2) rinse.--test portions were rinsed with pre-enrich ... | 2008 | 18980122 |
| compost and compost water extract-induced systemic acquired resistance in cucumber and arabidopsis. | abstract a biocontrol agent-fortified compost mix, suppressive to several diseases caused by soilborne plant pathogens, induced systemic acquired resistance (sar) in cucumber against anthracnose caused by colletotrichum orbiculare and in arabidopsis against bacterial speck caused by pseudomonas syringae pv. maculicola kd4326. a peat mix conducive to soilborne diseases did not induce sar. the population size of p. syringae pv. maculicola kd4326 was significantly lower in leaves of arabidopsis pla ... | 1998 | 18944926 |
| occurrence of indole-3-acetic acid-producing bacteria on pear trees and their association with fruit russet. | abstract a relatively high percentage of epiphytic bacteria on pear leaf and fruit surfaces had the ability to produce indole-3-acetic acid (iaa) in culture media supplemented with tryptophan. while over 50% of the strains produced at least small amounts of iaa in culture, about 25% of the strains exhibited high iaa production as evidenced by both colorimetric and high-performance liquid chromatography analysis of culture supernatants. a majority of the strains that produced high amounts of iaa ... | 1998 | 18944847 |
| effect of nectar on microbial antagonists evaluated for use in control of fire blight of pome fruits. | abstract under warm, dry conditions, erwinia amylovora can become established in relatively high populations on apple (malus domestica) or pear (pyrus communis) flower stigmas, and subsequent wet conditions facilitate its movement to the flower hypanthium where infection generally is initiated through the nectarthodes. research on biological control of fire blight has focused mainly on the flower stigma, and knowledge is lacking regarding the effect of nectar on microbial antagonists in the flow ... | 1999 | 18944801 |
| biological control of pseudomonas syringae pv. syringae, the causal agent of basal kernel blight of barley, by antagonistic pantoea agglomerans. | abstract strains of pantoea agglomerans (synanamorph erwinia herbicola) suppressed the development of basal kernel blight of barley, caused by pseudomonas syringae pv. syringae, when applied to heads prior to the pseudomonas syringae pv. syringae infection window at the soft dough stage of kernel development. field experiments in 1994 and 1995 revealed 45 to 74% kernel blight disease reduction, whereas glasshouse studies resulted in 50 to 100% disease control depending on the isolate used and ba ... | 2000 | 18944586 |
| a rapid bioassay for screening rhizosphere microorganisms for their ability to induce systemic resistance. | abstract we developed a rapid and miniaturized bioassay for screening large numbers of rhizosphere microorganisms for their ability to induce systemic resistance to bacterial leaf spot of radish caused by xanthomonas campestris pv. armoraciae. in this bioassay, pantoea agglomerans strain e278ar controlled symptoms of disease as effectively as 2,6-dichloroisonicotinic acid when applied to the roots of seedlings produced in growth pouches in a soilless system. e278ar essentially did not migrate fr ... | 2000 | 18944580 |
| an extracellular protease of pseudomonas fluorescens inactivates antibiotics of pantoea agglomerans. | abstract pseudomonas fluorescens a506 and pantoea agglomerans strains eh252 and c9-1 are biological control agents that suppress fire blight, an important disease of pear and apple caused by the bacterium erwinia amylovora. pseudomonas fluorescens strain a506 suppresses disease largely through competitive exclusion of e. amylovora on surfaces of blossoms, the primary infection court, whereas pantoea agglomerans strains eh252 and c9-1 produce antibiotics that are toxic to e. amylovora. in this st ... | 2004 | 18944458 |
| anatomy of cranberry stem gall and localization of bacteria in galls. | abstract cranberry stem gall is characterized by tumors that girdle stems, thereby killing all distal leaves, flowers, and fruit. bacteria that produce high levels of the plant growth hormone indole-3-acetic acid (iaa) are associated with and believed to cause cranberry stem gall. the anatomy of naturally occurring galls on woody cranberry plants and galls caused by inoculation of micropropagated cranberry plants with pantoea agglomerans strain 4/99 was consistent with elevated levels of iaa in ... | 2004 | 18944452 |
| assessment of environmental factors influencing growth and spread of pantoea agglomerans on and among blossoms of pear and apple. | abstract we evaluated effects of both physical and biological components of the environment on growth of pantoea agglomerans on inoculated pear and apple blossoms and on spread of the bacterium to blossoms on non-inoculated trees. the center three rows of 0.35- to 0.5-ha blocks of four pear cultivars and four apple cultivars were sprayed with a suspension of streptomycin-resistant p. agglomerans strain c9-1s (c9-1s) at 20 to 60% and 60 to 90% bloom. cultivars were chosen to create a sequence of ... | 2000 | 18944433 |
| antibiosis contributes to biological control of fire blight by pantoea agglomerans strain eh252 in orchards. | abstract fire blight, caused by erwinia amylovora, is the most serious bacterial disease of pear and apple trees. biological control with strains of pantoea agglomerans (syn. erwinia herbicola) may provide an effective disease management strategy for fire blight. most strains of p. agglomerans evaluated for suppression of fire blight produce compounds that inhibit the growth of e. amylovora in culture. the role of these inhibitory compounds in fire blight suppression in orchard environments has ... | 2002 | 18944246 |
| selection for quantitative trait loci associated with resistance to stewart's wilt in sweet corn. | the objectives of this research were to identify quantitative trait loci (qtl) for stewart's wilt resistance from a mapping population derived from a sweet corn hybrid that is highly resistant to pantoea stewartii and to determine if marker-based selection for those qtl could substantially improve stewart's wilt resistance in a population derived from a cross of resistant lines and a highly susceptible sweet corn inbred. three significant qtl for stewart's wilt resistance on chromosomes 2 (bin 2 ... | 2008 | 18944197 |
| comparative dynamics of adherent and nonadherent bacterial populations on maize leaves. | abstract the dynamics of the adherent and nonadherent populations of three bacterial species on maize leaves were examined to identify the extent to which bacteria adhere to leaves and the importance of this adhesion to leaf colonization. pantoea agglomerans strain brt98, clavibacter michiganensis subsp. nebraskensis strain gh2390, and pseudomonas syringae pv. syringae strain hs191r all rapidly adhered to maize leaves following inoculation, but differed in the percentage of cells that adhered to ... | 2002 | 18944027 |
| use of fatty acid methyl ester profiles to compare copper-tolerant and copper-sensitive strains of pantoea ananatis. | abstract a survey was conducted to evaluate differences in fatty acid methyl ester (fame) profiles among strains of pantoea ananatis, causal agent of center rot of onion (allium cepa), isolated from 15 different onion cultivars in three different sites in georgia. differences in fame composition were determined by plotting principal components (pcs) in two-dimensional plots. euclidean distance squared (ed(2)) values indicated a high degree of similarity among strains. plotting of pcs calculated ... | 2007 | 18943688 |
| antibiosis and acidification by pantoea agglomerans strain e325 may contribute to suppression of erwinia amylovora. | pantoea agglomerans strain e325, a commercially available antagonist for fire blight of apple and pear, was originally selected through screening based on suppression of erwinia amylovora on flower stigmas, but specific mechanisms of antagonism were unknown. bacterial modification of ph was evaluated as a possible mechanism by analyzing stigma exudates extracted from 'gala' apple stigmas. the ph values for field samples were only slightly lower than controls, but indicated a range (ph 5 to 6) co ... | 2008 | 18943460 |
| quantitative trait loci in sweet corn associated with partial resistance to stewart's wilt, northern corn leaf blight, and common rust. | abstract partial resistance to stewart's wilt (erwina stewartii, syn. pantoea stewartii), northern corn leaf blight (nclb) (exserohilum turcicum), and common rust (puccinia sorghi) was observed in an f(2:3) population developed from a cross between the inbred sweet corn lines il731a and w6786. the objective of this study was to identify quantitative trait loci (qtl) associated with partial resistance using restriction fragment length polymorphic markers. phenotypic data were collected for 2 year ... | 2001 | 18943349 |
| bacterial populations associated with rice seed in the tropical environment. | abstract during the 1995 wet season, harvested rice seed was collected from farmers' fields at different locations in iloilo, philippines. bacterial isolations from crushed seed yielded 428 isolates. the isolates were characterized by box-polymerase chain reaction fingerprinting of total genomic dna and represented 151 fingerprint types (fpt). most fpts were found on a single occasion, although matching fingerprints for isolates from different samples also were found. identifications were made b ... | 2001 | 18943348 |
| temporal dynamics of corn flea beetle populations infested with pantoea stewartii, causal agent of stewart's disease of corn. | abstract in order to better understand the epidemiology of the stewart's disease of corn pathosystem, quantitative information concerning the temporal dynamics of the amount of pathogen inoculum present in the form of pantoea stewartii-infested corn flea beetles (chaetocnema pulicaria) is needed. temporal changes in the proportion of p. stewartii-infested corn flea beetle populations were monitored by testing individual corn flea beetles for the presence of p. stewartii using a peroxidase-labele ... | 2003 | 18943136 |
| temperature and pomaceous flower age related to colonization by erwinia amylovora and antagonists. | abstract fire blight of apple and pear is initiated by epiphytic populations of erwinia amylovora on flower stigmas. predicting this disease and managing it with microbial antagonists depends on an understanding of bacterial colonization on stigmas. detached 'manchurian' crab apple flowers were inoculated with e. amylovora and subjected to a range of constant temperatures or various fluctuating temperature regimes. results may have application to disease risk assessment systems such as the couga ... | 2004 | 18943112 |
| role of blossoms in watermelon seed infestation by acidovorax avenae subsp. citrulli. | abstract the role of watermelon blossom inoculation in seed infestation by acidovorax avenae subsp. citrulli was investigated. approximately 98% (84/87) of fruit developed from blossoms inoculated with 1 x 10(7) or 1 x 10(9) cfu of a. avenae subsp. citrulli per blossom were asymptomatic. using immunomagnetic separation and the polymerase chain reaction, a. avenae subsp. citrulli was detected in 44% of the seed lots assayed, despite the lack of fruit symptoms. furthermore, viable colonies were re ... | 2003 | 18942974 |
| pantoea agglomerans bloodstream infection in preterm neonates. | objective: to report an uncommon incidence of sporadic bloodstream infection (bsi) caused by pantoea agglomerans in preterm neonates. case presentation and intervention: fives cases of nosocomial bsi with p. agglomerans in preterm neonates (weight <or=1,500 g; age 8-17 days; gestational age 26-30 weeks) are presented. all cases were late onset neonatal sepsis (>7 days of age). lethargy, skin mottling and bradycardia were often present. although there was no evidence of pneumonia, desaturation wa ... | 2008 | 18836282 |
| biosynthesis of the vitamin e compound delta-tocotrienol in recombinant escherichia coli cells. | the biosynthesis of natural products in a fast growing and easy to manipulate heterologous host system, such as escherichia coli, is of increasing interest in biotechnology. this procedure allows the investigation of complex natural product biosynthesis and facilitates the engineering of pathways. here we describe the cloning and the heterologous expression of tocochromanol (vitamin e) biosynthesis genes in e. coli. tocochromanols are synthesized solely in photosynthetic organisms (cyanobacteria ... | 2008 | 18810749 |
| phylogenetic placement of pentatomid stink bug gut symbionts. | insect bacterial symbionts are ubiquitous, however, only a few groups of host families have been well studied in relation to their associations with microbes. the determination of the phylogenetic relationships among bacteria associated with different species within an insect family can provide insights into the biology and evolution of these interactions. we studied the phylogenetic placement of vertically transmitted bacterial symbionts associated with the posterior midgut (crypt-bearing) regi ... | 2009 | 18810535 |
| pantoea stewartii subsp. stewartii exhibits surface motility, which is a critical aspect of stewart's wilt disease development on maize. | pantoea stewartii subsp. stewartii is a plant-pathogenic bacterium that causes stewart's vascular wilt in maize. the organism is taxonomically described as aflagellated and nonmotile. we recently showed that p. stewartii colonizes the xylem of maize as sessile, cell-wall-adherent biofilms. biofilm formation is a developmental process that generally involves some form of surface motility. for that reason, we reexamined the motility properties of p. stewartii dc283 based on the assumption that the ... | 2008 | 18785831 |
| in vitro adsorption revealing an apparent strong interaction between endophyte pantoea agglomerans ys19 and host rice. | pantoea (formerly enterobacter) agglomerans ys19 is a dominant diazotrophic endophyte isolated from rice (oryza sativa cv. yuefu) grown in a temperate-climate region in west beijing, china. in vitro adsorption and invasion of ys19 on host plant root were studied in this research. adsorption of ys19 on rice seedling roots closely resembled the langmuir adsorption and showed a higher adsorption quantity than the control strains paenibacillus polymyxa wy110 (a rhizospheric bacterium from the same r ... | 2008 | 18781359 |
| case report: subacute synovitis of the knee after a rose thorn injury: unusual clinical picture. | synovitis secondary to penetrating plant thorn injuries is not frequently reported. historically, it is considered aseptic and treated with removal of the intraarticular foreign body and affected synovial lining. we report a 57-year-old healthy man who was admitted 2 weeks after being injured by a rose (rosacea) thorn with subacute and mild synovitis with effusion of his right knee. no intraarticular foreign body was retained. pantoea agglomerans was identified in the synovial fluid. contrary to ... | 2008 | 18773251 |
| andrimid producers encode an acetyl-coa carboxyltransferase subunit resistant to the action of the antibiotic. | andrimid is a hybrid nonribosomal peptide-polyketide antibiotic that blocks the carboxyl-transfer reaction of bacterial acetyl-coa carboxylase (acc) and thereby inhibits fatty acid biosynthesis with submicromolar potency. the andrimid biosynthetic gene cluster from pantoea agglomerans encodes an admt gene with homology to the acetyl-coa carboxyltransferase (ct) beta-subunit gene accd. escherichia coli cells overexpressing admt showed resistance to andrimid. co-overproduction of admt with e. coli ... | 2008 | 18768797 |
| endophytic population of pantoea agglomerans in citrus plants and development of a cloning vector for endophytes. | harmless bacteria inhabiting inner plant tissues are termed endophytes. population fluctuations in the endophytic bacterium pantoea agglomerans associated with two species of field cultured citrus plants were monitored over a two-year period. the results demonstrated that populations of p. agglomerans fluctuated in citrus reticulata but not c. sinensis. a cryptic plasmid ppa3.0 (2.9 kb) was identified in 35 out of 44 endophytic isolates of p. agglomerans and was subsequently sequenced. the origi ... | 2008 | 18759238 |
| diversity of auxin-producing bacteria associated to pseudomonas savastanoi -induced olive knots. | forty three strains were isolated from knots induced by pseudomonas savastanoi in different olive cultivars. all the selected bacteria were shown to produce variable amounts of the plant growth hormone indole-3-acetic acid (iaa). amplification of the intergenic transcribed spacers (its) between 16s and 23s rdna genes, allowed the clustering of the isolates into seven distinct groups. all isolates from its group 1 were positive to the pseudomonas savastanoi pv. savastanoi specific iaa l gene as s ... | 2008 | 18759227 |
| diversity of endophytic enterobacteria associated with different host plants. | fifty-three endophytic enterobacteria isolates from citrus, cocoa, eucalyptus, soybean, and sugar cane were evaluated for susceptibility to the antibiotics ampicillin and kanamycin, and cellulase production. susceptibility was found on both tested antibiotics. however, in the case of ampicillin susceptibility changed according to the host plant, while all isolates were susceptible to kanamycin. cellulase production also changed according to host plants. the diversity of these isolates was estima ... | 2008 | 18758726 |
| diversity of endophytic bacteria from eucalyptus species seeds and colonization of seedlings by pantoea agglomerans. | the diversity and beneficial characteristics of endophytic microorganisms have been studied in several host plants. however, information regarding naturally occurring seed-associated endophytes and vertical transmission among different life-history stages of hosts is limited. endophytic bacteria were isolated from seeds and seedlings of 10 eucalyptus species and two hybrids. the results showed that endophytic bacteria, such as bacillus, enterococcus, paenibacillus and methylobacterium, are verti ... | 2008 | 18710397 |
| [intestinal enterobacteria of the hibernating apis mellifera mellifera l. bees]. | dynamics of enterobacteria of normal intestinal microflora was studied in apis mellifera mellifera l. bees hibernating under snow in the western urals. the cell numbers (n) of the predominant species klebsiella oxytoca increased from 10-10(6) cfu/bee in november 2004 to 10(4)-10(7) cfu/bee in march 2005; its frequency of occurrence (p) increased from 92 to 100%. increase of providencia rettgeri (11.2004: n up to 10(6), p 25%; 03.2005: n 10(2)-10(6), p 80%) was accompanied by the substitution of ... | 2008 | 18683661 |
| transstadial and horizontal transfer of bacteria within a colony of anopheles gambiae (diptera: culicidae) and oviposition response to bacteria-containing water. | in a paratransgenic approach, genetically modified bacteria are utilized to kill the parasite in the vector gut. a critical component for paratransgenics against malaria is how transgenic bacteria can be introduced and then kept in a mosquito population. here, we investigated transstadial and horizontal transfer of bacteria within an anopheles gambiae mosquito colony with the focus on spiked breeding sites as a possible means of introducing bacteria to mosquitoes. a pantoea stewartii strain, pre ... | 2008 | 18671931 |
| comparative analysis of antibiotic resistance characteristics of gram-negative bacteria isolated from laying hens and eggs in conventional and organic keeping systems in bavaria, germany. | by investigating the prevalence and resistance characteristics of gram-negative bacteria from organic and conventional kept laying hens against 31 (campylobacter: 29) different antibiotics using the microdilution method, we determined to what extent different keeping systems influence bacterial resistance patterns. for this purpose, samples from 10 organic and 10 conventional flocks in bavaria (germany) were investigated four times between january 2004 and april 2005. altogether, 799 cloacal swa ... | 2008 | 18667026 |
| quorum-sensing signals in the microbial community of the cabbage white butterfly larval midgut. | the overall goal of this study was to examine the role of quorum-sensing (qs) signals in a multispecies microbial community. toward this aim, we studied qs signals produced by an indigenous member and an invading pathogen of the microbial community of the cabbage white butterfly (cwb) larval midgut (pieris rapae). as an initial step, we characterized the qs system in pantoea cwb304, which was isolated from the larval midgut. a luxi homolog, designated pani, is necessary for the production of n-a ... | 2008 | 18650927 |
| arsenite oxidation by alcaligenes sp. strain rs-19 isolated from arsenic-contaminated mines in the republic of korea. | arsenite [as(iii)]-oxidizing bacteria play important roles in reducing arsenic [as] toxicity and mobility in as-contaminated areas. as-resistant bacteria were isolated from the soils of two abandoned mines in the republic of korea. the isolated bacteria showed relatively high resistances to as(iii) up to 26 mm. the pcr-based 16s rrna analysis revealed that the isolated as-resistant bacteria were close relatives to serratia marcescensa, pseudomonas putida, pantoea agglomerans, and alcaligenes sp. ... | 2009 | 18642094 |
| enrichment of carotenoids in flaxseed (linum usitatissimum) by metabolic engineering with introduction of bacterial phytoene synthase gene crtb. | linseed flax (linum usitatissimum l.) is an industrially important oil crop, which includes large amounts of alpha-linolenic acid (18:3) and lignan in its seed oil. we report here the metabolic engineering of flax plants to increase carotenoid amount in seeds. agrobacterium-mediated transformation of flax was performed to express the phytoene synthase gene (crtb) derived from the soil bacterium pantoea ananatis (formerly called erwinia uredovora 20d3) under the control of the cauliflower mosaic ... | 2008 | 18640603 |
| bacteremia in equine neonatal diarrhea: a retrospective study (1990-2007). | bacteremia in sick foals is associated with survival, but the association of bacteremia and diarrhea is not reported. | 2008 | 18638014 |
| persistence of salmonella senftenberg in poultry production environments and investigation of its resistance to desiccation. | most salmonella serovars, including salmonella enterica subsp. enterica serovar senftenberg (s. senftenberg), are tolerant to desiccation and able to colonize and persist in feed mills. in addition, they may survive cleaning and disinfection procedures used on poultry farms. the present study was conducted to investigate the survival of s. senftenberg in broiler parent stock farms and broiler farms. the isolates from one of the parent stock farms investigated only differed by a single band in fl ... | 2008 | 18622860 |
| the product chain length determination mechanism of type ii geranylgeranyl diphosphate synthase requires subunit interaction. | the product chain length determination mechanism of type ii geranylgeranyl diphosphate synthase from the bacterium, pantoea ananatis, was studied. in most types of short-chain (all-e) prenyl diphosphate synthases, bulky amino acids at the fourth and/or fifth positions upstream from the first aspartate-rich motif play a primary role in the product determination mechanism. however, type ii geranylgeranyl diphosphate synthase lacks such bulky amino acids at these positions. the second position upst ... | 2008 | 18616462 |
| quorum-sensing system affects gall development incited by pantoea agglomerans pv. gypsophilae. | the quorum-sensing (qs) regulatory system of the gall-forming pantoea agglomerans pv. gypsophilae was identified. mass spectral analysis, together with signal-specific biosensors, demonstrated that p. agglomerans pv. gypsophilae produced n-butanoyl-l-homoserine lactone (c4-hsl) as a major and n-hexanoyl-l-homoserine lactone (c6-hsl) as a minor qs signal. homologs of luxi and luxr regulatory genes, pagi and pagr, were characterized in strain p. agglomerans pv. gypsophilae pag824-1 and shown to be ... | 2008 | 18616406 |
| new erwinia-like organism causing cervical lymphadenitis. | the first case of cervical lymphadenitis due to infection by a new erwinia-like organism is reported. the organism was identified initially as pantoea sp. by a vitek 2-based assessment but was finally identified as a member of the genus erwinia by 16s rrna gene sequence analysis. the isolate displayed 98.9% 16s rrna gene sequence similarity to that of e. tasmaniensis and showed phenotypic characteristics that were different from other erwinia species. | 2008 | 18614665 |
| in vitro study of pro-inflammatory and anti-tumour properties of microvesicles from bacterial cell wall of pantoea agglomerans. | in the environment, gram-negative bacteria are capable of producing large amounts of endotoxin-containing microvesicles - spherical structures measuring 30-50 nm in diameter, emerging by fragmentation of the outer membrane of the bacterial cell wall. microvesicles are suspected of inducing inflammatory lung diseases, but possibly also of stimulating anti-tumour defence mechanisms. the present study was aimed at assessing the pro-inflammatory and anti-tumour properties of microvesicles in vitro. ... | 2008 | 18581995 |
| pantoea peritonitis: not just a "thorny" problem. | 2008 | 18556396 | |
| pantoea agglomerans: an unusual inciting agent in peritonitis. | 2008 | 18556394 | |
| short peptide induces an "uncultivable" microorganism to grow in vitro. | microorganisms comprise the bulk of biodiversity, but only a small fraction of this diversity grows on artificial media. this phenomenon was noticed almost a century ago, repeatedly confirmed, and termed the "great plate count anomaly." advances in microbial cultivation improved microbial recovery but failed to explain why most microbial species do not grow in vitro. here we show that at least some of such species can form domesticated variants capable of growth on artificial media. we also pres ... | 2008 | 18515474 |
| ribosomal rna-based analysis of the bacterial flora from the conjunctivae of cattle with bovine keratoconjunctivitis (bkc). | bovine keratoconjunctivitis (bkc), colloquially referred to as 'pinkeye', is a disease affecting cattle worldwide; it costs cattle producers millions of dollars in economic loss annually. while moraxella spp. are the primary etiologic agent of pinkeye, surveys of flora from the conjunctivae of livestock from around the world have indicated that a variety of bacterial commensals occupy this niche. we used molecular biology-based methods to determine the composition of bacterial flora in the conju ... | 2008 | 18513895 |
| is 16s rdna a reliable phylogenetic marker to characterize relationships below the family level in the enterobacteriaceae? | the phylogenetic relationships of multiple enterobacterial species were reconstructed based on 16s rdna gene sequences to evaluate the robustness of this housekeeping gene in the taxonomic placement of the enteric plant pathogens erwinia, brenneria, pectobacterium, and pantoea. four data sets were compiled, two of which consisted of previously published data. the data sets were designed in order to evaluate how 16s rdna gene phylogenies are affected by the use of different plant pathogen accessi ... | 2008 | 18504519 |
| response of endophytic bacterial communities in banana tissue culture plantlets to fusarium wilt pathogen infection. | endophytic bacteria reside within plant hosts without having pathogenic effects, and various endophytes have been found to functionally benefit plant disease suppressive ability. in this study, the influence of banana plant stress on the endophytic bacterial communities, which was achieved by infection with the wilt pathogen fusarium oxysporum f. sp. cubense, was examined by cultivation-independent denaturing gradient gel electrophoresis analysis of 16s ribosomal dna directly amplified from plan ... | 2008 | 18497482 |
| absolute configuration and antimicrobial activity of acylhomoserine lactones. | (s)-n-heptanoylhomoserine lactone is an uncommon acyl odd-chain natural product employed by many gram-negative bacteria as a signaling substance in chemical communication mechanisms known as quorum sensing. the absolute configuration determination of the metabolite produced by the phytopathogen pantoea ananatis serrano is reported herein. as with all other substances of this class, the lactone moiety possesses s configuration, corroborating the hypothesis that it shares the same biosynthetic pat ... | 2008 | 18465897 |
| dgge and real-time pcr analysis of lactic acid bacteria in bacterial communities of the phyllosphere of lettuce. | food associated indigenous microbial communities exert antagonistic effects on pathogens and may routinely deliver health relevant microorganisms to the gi tract. by using molecular, culture independent methods including pcr-dgge of 16s rdna-coding regions and real-time pcr (rt-pcr) as well as biolog metabolic fingerprinting, microbial communities on lettuce were analyzed in samples from fields, from supermarkets and soil. amplified 16s rrna gene sequences (57.7%) could be assigned to species pr ... | 2008 | 18398868 |
| evaluation of antifungal activity of carbonate and bicarbonate salts alone or in combination with biocontrol agents in control of citrus green mold. | the aim of this research was to determine if the attacks of green mold on orange could be reduced by edible salts alone or in combination with biocontrol agent. for this purpose toxicity to pantoea digitatum and practical use of sodium carbonate (sc), sodium bicarbonate (sbc) and potassium carbonate, and potassium bicarbonate alone or in combination with antagonistic bacteria (pseudomonas fluorescens isolate pn, bacillus subtilis isolate vhn, pantoea agglomerans isolate ca) to control green mold ... | 2007 | 18396809 |
| growth-inhibitory effects of sulfonamides at different ph: dissimilar susceptibility patterns of a soil bacterium and a test bacterium used for antibiotic assays. | the ionic speciation of sulfonamides is ph-driven and this may be crucial for their bioavailability and sorption to soil constituents, as well as for their uptake into bacterial cells. the inhibition behaviour of a bacterial test strain (pseudomonas aeruginosa; dsm 1117), which was grown in the presence of different concentrations of 8 sulfonamides at ph values from 5 to 8, could be predicted by models that take the speciation of sulfonamides in- and outside of bacterial cells into account. assu ... | 2008 | 18396316 |
| potential sources of microbial contamination of satsuma mandarin fruit in japan, from production through packing shed. | potential sources of microbial contamination of satsuma mandarin fruit were investigated from production through the packing shed in the 2005 season. microbial counts in the peel and flesh during the fruit development stage were below 2.4 log cfu/g for bacteria and 3 log cfu/g for fungi, except for the peel in august and september. in the field environment, the highest microbial counts were found in fallen leaves on the ground, followed by soil, organic fertilizer, and agricultural water. only t ... | 2008 | 18389696 |
| genetic diversity of endophytic bacteria which could be find in the apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants. | the genetic diversity of 29 endophytic bacterial strains isolated from apoplastic sap of the medullary parenchym of the stem of healthy sugarcane plants grown in cuba was analysed by two primers-ramdom amplified polymorphic dna fingerprinting (tp-rapd) and 16s rrna gene sequencing. the strains were distributed into 17 groups on the basis of their tp-rapd patterns, and a representative strain from each group was subjected to 16s rrna gene sequencing. analysis of these sequences showed that the is ... | 2008 | 18383223 |
| [study of ectoparasitism of ultramicrobacteria of the genus kaistia, strains nf1 and nf3 by electron and fluorescence microscopy]. | transmission electron and fluorescence microscopy was used to study the character of the interaction of free-living ultramicrobacterial (umb) strains nf1 and nf3, affiliated with the genus kaistia, and seven species of gram-positive and gram-negative heterotrophic bacteria. strains nf1 and nf3 were found to exhibit parasitic activity against gram-positive bacillus subtilis and gram-negative acidovorax delafildii. umb cells are tightly attached to the envelopes of the victim cells and induce thei ... | 2008 | 18365722 |
| bacteria associated with ectomycorrhizas of slash pine (pinus elliottii) in south-eastern queensland, australia. | bacterial communities associated with ectomycorrhizal and uncolonized roots of pinus elliottii (slash pine) collected from a plantation in south-east queensland, australia, were investigated, using cultivation-dependent and -independent methods. denaturing gradient gel electrophoresis (dgge) analysis of 16s rrna gene pcr products obtained using a cultivation-independent approach revealed that bacterial communities associated with ectomycorrhizal root tips differed significantly from those associ ... | 2008 | 18355286 |
| stabilization of psw100 from pantoea stewartii by the f conjugation system. | plasmid psw100 is 1 of the 13 plasmids from pantoea stewartii subsp. stewartii sw2 which has a replicon that resembles that of cole1. this work uses a psw100 derivative, psw140k, to study how the psw100 replicon is stably maintained in its hosts. our results indicate that although psw140k is stable in escherichia coli hb101, the plasmid is rapidly lost in another e. coli strain, dh5alpha, indicating that the genetic background of an e. coli strain affects the stability of psw140k. mutagenesis of ... | 2008 | 18344358 |