Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| [diversity of bacteria associated with pine wood nematode revealed by metagenome]. | the pine wood nematode (pwn), bursaphlenchus xylophilus, which collaborates with its associated bacteria to form ecosystem and has interaction among them, is the pathogen of pine wilt disease. this study focused on revealing the bacterial diversity of ecosystem of pine wood nematode and its associated bacteria. | 2010 | 20815238 |
| [physiological properties and taxonomic position of soil microorganisms-destructors of hexachlorcyclohexane]. | eleven (11) microbial strains capable to decompose insecticide hexachlorcyclohexane (hch) have been isolated from microbial association resistant to chloro-organic pesticides. on the basis of investigation of morphological, cultural, physiological, biochemical characteristics and results of sequence of gene 16s rrna it has been established, that the best strain-destructors n 3 and n 9 belong to pseudomonas putida species, and strain n 6 belongs to stenotrophomonas maltophilia species. | 2010 | 20812504 |
| [taxonomic position of certain representatives of sulphate-reducing corrosive microbial community]. | sulfate-reducing bacteria and their heterotrophic satellites have been isolated from the biofilm formed on steel by sulfidogenic corrosive microbial community. bacteria were characterized according to phenotypical features and investigated by the methods of molecular-genetic analysis. in accordance with the phenotypical features the studied strain of sulfate-reducing bacteria were related to desulfovibrio genus, gram-positive strains of heterotrophic satellites were related to bacillus genus, b. ... | 2010 | 20812503 |
| stenotrophomonas maltophilia as a causal agent of pyogranulomatous hepatitis in a buffalo (bubalus bubalis). | a 7-year-old female buffalo (bubalus bubalis) from a local herd in serres, northern greece, was presented to a private veterinary clinic with a chronic loss of appetite for 15 days. the clinical examination revealed high fever (41.5 degrees c), lethargy, yellow discoloration of skin and mucous membranes, an abdomen that appeared to be empty, hyperactive rumen motility, and tachypnea. a biochemical profile revealed an elevated total bilirubin concentration and hepatic enzyme activities, whereas g ... | 2010 | 20807941 |
| diversity of cold-active protease-producing bacteria from arctic terrestrial and marine environments revealed by enrichment culture. | a new approach for enrichment culture was applied to obtain cold-active protease-producing bacteria for marine and terrestrial samples from svalbard, norway. the method was developed for the enrichment of bacteria by long-term incubation at low temperatures in semi-solid agar medium containing meat pieces as the main source of carbon and energy. zobell and 0.1x nutrient broth were added for marine and terrestrial microorganisms, respectively, to supply basal elements for growth. one to three typ ... | 2010 | 20799082 |
| treatment of recurrent stenotrophomonas maltophilia ventilator-associated pneumonia with doxycycline and aerosolized colistin. | to report a case of recurrent stenotrophomonas maltophilia ventilator-associated pneumonia (vap) that was successfully treated with doxycycline and aerosolized colistin. | 2010 | 20736426 |
| a cluster of acute-onset postoperative endophthalmitis over a 1-month period: investigation of an outbreak caused by uncommon species. | to report the clinical course, treatment response and prognosis of eight cases which developed acute-onset postoperative endophthalmitis over a 1-month period. | 2011 | 20733020 |
| anaerobic oxidation of arsenite linked to chlorate reduction. | microorganisms play a significant role in the speciation and mobility of arsenic in the environment. in this study, the oxidation of arsenite [as(iii)] to arsenate [as(v)] linked to chlorate (clo₃⁻) reduction was shown to be catalyzed by sludge samples, enrichment cultures (ecs), and pure cultures incubated under anaerobic conditions. no activity was observed in treatments lacking inoculum or with heat-killed sludge, or in controls lacking clo₃⁻. the as(iii) oxidation was linked to the complete ... | 2010 | 20729322 |
| stenotrophomonas maltophilia endocarditis of prosthetic mitral valve. | we present a 78-year-old woman with prosthetic valve endocarditis due to stenotrophomonas maltophilia (s. maltophilia) 3 years after mitral valve replacement. administration of sulfamethoxazole-trimethoprim was effective; however, it was discontinued because of side effects, which led to failure of antibiotic therapy. complications of multiple cerebral infarction and paravalvular abscess developed. although the prosthetic valve was removed 50 days after admission, she died 4 months after surgery ... | 2010 | 20720357 |
| biocidal efficacy of silver-impregnated contact lens storage cases in vitro. | silver-impregnated contact lens (cl) storage cases are designed to reduce microbial contamination during use, but there are limited data on their effectiveness. this study evaluated early antimicrobial activity of silver-impregnated cl cases and silver-release characteristics in vitro. | 2011 | 20720221 |
| infections occurring during extracorporeal membrane oxygenation use in adult patients. | the application of extracorporeal membrane oxygenation in adults has been increasing, but infections occurring during extracorporeal membrane oxygenation use are rarely described. | 2010 | 20708754 |
| [isolation, identification, and degrading characteristics of a high-efficient pyrene-degrading bacterial strain]. | by using selective enrichment culture with pyrene as the sole carbon source, a pyrene-degrading bacterial strain zq5 was isolated from the oil-contaminated soil in shenfu irrigation area. the strain was identified as stenotrophomonas sp., based on its morphological, physiological, and biochemical characteristics, and similarity identification of 16s rdna sequence. the pyrene-degrading characteristics and the effects of culture condition on the degrading efficiency of the strain were investigated ... | 2010 | 20707122 |
| prevalence of antimicrobial resistance among gram-negative isolates in an adult intensive care unit at a tertiary care center in saudi arabia. | patients in the icu have encountered an increasing emergence and spread of antibiotic-resistant pathogens. we examined patterns of antimicrobial susceptibility in gram-negative isolates to commonly used drugs in an adult icu at a tertiary care hospital in riyadh, saudi arabia. | 2010 | 20697174 |
| [biofilm on a metal surface as a factor of microbial corrosion]. | main attention was given in the present review to the research methods, phases of biofilm's forming, exopolymer compounds of bacteria as main biofilm forming factor. a microbial corrosion as a result of interaction between the biofilm and metal surface was considered. the interaction was displayed in biomineralization. the future trends of biofilms study were bound with research of their architecture. that architecture was determined by the structure and function of biofilms compounds: biopolyme ... | 2010 | 20695231 |
| rapid detection of pseudomonas aeruginosa from positive blood cultures by quantitative pcr. | pseudomonas aeruginosa is responsible for numerous bloodstream infections associated with severe adverse outcomes in case of inappropriate initial antimicrobial therapy. the present study was aimed to develop a novel quantitative pcr (qpcr) assay, using ecfx as the specific target gene, for the rapid and accurate identification of p. aeruginosa from positive blood cultures (bcs). | 2010 | 20684778 |
| post-antibiotic effect (pae) of moxifloxacin in multidrug-resistant stenotrophomonas maltophilia. | 2010 | 20674284 | |
| [investigation of distribution of bacteria and fungi in severe acute pancreatitis]. | to investigate the spectrum of bacteria and fungi in different sites in severe acute pancreatitis (sap). | 2010 | 20646657 |
| diffusion measurements inside biofilms by image-based fluorescence recovery after photobleaching (frap) analysis with a commercial confocal laser scanning microscope. | research about the reactional and structural dynamics of biofilms at the molecular level has made great strides, owing to efficient fluorescence imaging methods in terms of spatial resolution and fast acquisition time but also to noninvasive conditions of observation consistent with in situ biofilm studies. in addition to conventional fluorescence intensity imaging, the fluorescence recovery after photobleaching (frap) module can now be routinely implemented on commercial confocal laser scanning ... | 2010 | 20639359 |
| newer antibacterial agents and their potential role in cystic fibrosis pulmonary exacerbation management. | pulmonary exacerbations in cystic fibrosis (cf) are frequent events and account for a substantial proportion of the burden of morbidity and mortality in this disease. antibacterial therapies to treat pulmonary exacerbations are instituted empirically and are individualized based on both patient factors (severity of exacerbation, frequency of exacerbation, recent courses of anti-infectives) and pathogen factors (previously isolated pathogens and in vitro predicted susceptibilities). however, the ... | 2010 | 20605846 |
| [analysis of acquired resistance genes in stenotrophomonas maltophilia]. | stenotrophomonas maltophilia is a gram-negative bacillus and a nosocomial pathogen in immunocompromised patients. trimethoprim/sulfamethoxazole (tmp/smx) is the drug of choice for treating s. maltophilia infection; however, resistance to tmp/smx is increasing. in this study, we investigated the relationship between the incidence of tmp/smx resistance and the presence of sul genes and mobile elements. | 2010 | 20603591 |
| isolation, characterization and phylogeny of sponge-associated bacteria with antimicrobial activities from brazil. | bacteria associated with marine sponges represent a rich source of bioactive metabolites. the aim of this study was to isolate and characterize bacteria with antimicrobial activities from brazilian sponges. a total of 158 colony-forming units were isolated from nine sponge species. among these, 12 isolates presented antimicrobial activities against pathogenic bacteria. based on comparative sequence analysis of their 16s rrna genes, the sponge-associated bacterial strains could be subdivided into ... | 2010 | 20600863 |
| microbial etiologies of hospital-acquired bacterial pneumonia and ventilator-associated bacterial pneumonia. | hospital-acquired bacterial pneumonia (habp) and ventilator-associated bacterial pneumonia (vabp) can be caused by a wide variety of bacteria that originate from the patient flora or the health care environment. we review the medical and microbiology literature and the results of the sentry antimicrobial surveillance program (1997-2008) to establish the pathogens most likely to cause habp or vabp. in all studies, a consistent 6 organisms (staphylococcus aureus [28.0%], pseudomonas aeruginosa [21 ... | 2010 | 20597676 |
| tertiary structure-related activity of tick defensin (persulcatusin) in the taiga tick, ixodes persulcatus. | defensins are small cysteine-rich cationic proteins found in both vertebrates and invertebrates constituting the front line of host innate immunity. to examine the importance of the tertiary structure of tick defensin in its antimicrobial activity, we synthesized two types of the peptides with tertiary structure or primary one on basis of the information of the sequence in the defensin originated from the taiga tick, ixodes persulcatus. chemically synthesized peptides were used to investigate th ... | 2011 | 20596886 |
| case report 6: candidaemia in a high risk liver transplant recipient. | 2010 | 20591016 | |
| antibiotic resistance of gram negative bacilli strains isolated from the intensive care unit in fundeni clinical institute, bucharest, romania. | the aim of this study was to investigate the antibiotic resistance profile of 58 gram negative bacilli strains (gnb): 36 non-fermentative gnb (ngnb), including 19 strains of acinetobacter spp., 11 of pseudomonas aeruginosa, 6 of stenotrophomonas maltophilia and 22 enterobacterial strains (14 strains of kehss, 6 belonging to the group proteus-providencia and 2 escherichia coli) isolated from nasal, pharyngeal exudates and also from bronchial secretions, from immuno-depressed patients admitted in ... | 2009 | 20583477 |
| [interpretive reading of the non-fermenting gram-negative bacilli antibiogram]. | among non-fermenting gram-negative rods, the most clinically important species are pseudomonas aeruginosa, acinetobacter baumannii, and stenotrophomonas maltophilia, which are frequently multiresistant. p. aeruginosa resistance to beta-lactams depends on the production of chromosomal and plasmid-mediated beta-lactamases, altered permeability (loss of oprd porin is related to carbapenem-resistance) and active efflux systems, particularly mexab-oprm. in aminoglycoside resistant strains, the main m ... | 2010 | 20579775 |
| clinical profile of adult cystic fibrosis patients with frequent epidemic clones of pseudomonas aeruginosa. | earlier reports suggested that pseudomonas aeruginosa frequent epidemic clones circulating in cystic fibrosis (cf) centres had increased virulence. however, recent data show no consistent associations with virulence, and suggest attenuation of virulence in chronic infection. changes to infection control programmes in relation to frequent epidemic clones should be based on their frequency, virulence across all age groups and mode of acquisition. the australian epidemic strain-1 (aes-1) (or the me ... | 2010 | 20573059 |
| [endocarditis due to stenotrophomonas maltophilia and enterobacter cloacae]. | 2010 | 20570073 | |
| evaluation of genetic and functional diversity of stenotrophomonas isolates from diverse effluent treatment plants. | in this study, the samples were collected from nine etps and soil contaminated with petroleum products. the genetic diversity of 30 stenotrophomonas isolates was demonstrated by phylogenetic analysis of their 16s rrna gene nucleotide sequences, and randomly amplified polymorphic dna (rapd) analysis supplemented with in silico signature and restriction enzyme (res - alui, bfai, dpnii, haeiii, rsai and tru9i) digestion analyses. genetic diversity based on nucleotide sequence data revealed distinct ... | 2010 | 20554196 |
| analysis of bacterial fatty acids by flow modulated comprehensive two-dimensional gas chromatography with parallel flame ionization detector/mass spectrometry. | comprehensive two-dimensional gas chromatography (gcxgc) offers an interesting tool for profiling bacterial fatty acids. flow modulated gcxgc using a commercially available system was evaluated, different parameters such as column flows and modulation time were optimized. the method was tested on bacterial fatty acid methyl esters (bames) from stenotrophomonas maltophilia lmg 958(t) by using parallel flame ionization detector (fid)/mass spectrometry (ms). the results are compared to data obtaine ... | 2010 | 20546760 |
| in vitro synergistic/additive activity of levofloxacin with meropenem against stenotrophomonas maltophilia. | synergy testing of levofloxacin and meropenem by etest and time-kill assay (tka) was performed against 30 genetically unique clinical stenotrophomonas maltophilia isolates. synergy was demonstrated in 18/30 (60%) isolates by etest and in 13/30 (43%) by tka; the remaining isolates were indifferent. methods showed agreement for 25/30 (83%) of isolates. | 2010 | 20542209 |
| the role of stenotrophomonas maltophilia in refractory chronic rhinosinusitis. | stenotrophomonas maltophilia is a multidrug-resistant gram-negative bacillus that has been implicated in serious nosocomial infections. this microbe has also been isolated from sinus cultures in refractory chronic rhinosinusitis (crs). the goal of this study was to elucidate the implications of s. maltophilia-positive cultures in the setting of crs. the objectives of the study were (1) to define clinical and microbiological characteristics and (2) to assess management strategy and overall outcom ... | 2010 | 20537287 |
| a giant family of short palindromic sequences in stenotrophomonas maltophilia. | the genome of stenotrophomonas maltophilia is peppered with palindromic elements called smag (stenotrophomonas maltophilia gtag) because they carry at one terminus the tetranucleotide gtag. the repeats are species-specific variants of the superfamily of repetitive extragenic palindromes (reps), dna sequences spread in the intergenic space in many prokaryotic genomes. the genomic organization and the functional features of smag elements are described herein. a total of 1650 smag elements were ide ... | 2010 | 20528935 |
| analysis of bacterial fatty acids by flow modulated comprehensive two-dimensional gas chromatography with parallel flame ionization detector/mass spectrometry. | comprehensive two-dimensional gas chromatography (gcxgc) offers an interesting tool for profiling bacterial fatty acids. flow modulated gcxgc using a commercially available system was evaluated, different parameters such as column flows and modulation time were optimized. the method was tested on bacterial fatty acid methyl esters (bames) from stenotrophomonas maltophilia lmg 958t by using parallel flame ionization detector (fid)/mass spectrometry (ms). the results are compared to data obtained ... | 2010 | 20527679 |
| stenotrophomonas maltophilia: significant contemporary hospital pathogen - review. | stenotrophomonas maltophilia (sm) plays an important role as an opportunistic pathogen in immunocompromised individuals. the growing detection rates of this bacterium in hospitalized patients are associated with the invasiveness of therapeutic and diagnostic procedures and the selection pressure of antibiotic therapy. a broad range of infections that can be caused by sm is frequently bound to biofilm. the high level of intrinsic resistance to many unrelated antibiotics and increasing acquired re ... | 2010 | 20526844 |
| ecthyma gangrenosum: a rare cutaneous manifestation caused by stenotrophomonas maltophilia in a leukemic patient. | ecthyma gangrenosum (eg) is a well-recognized cutaneous infection that most commonly affects immunocompromised patients. it typically occurs on the extremities, or in gluteal and perineal regions. although pseudomonas aeruginosa is the most well-known pathogen causing eg, other organisms have been reported to cause eg. herein we report a rare case of ecthyma gangrenosum presenting as aggressive necrotic skin lesions in perioral and infraorbital areas in a 47-year-old patient with acute myelocyti ... | 2009 | 20523829 |
| structure of stenotrophomonas maltophilia feoa complexed with zinc: a unique prokaryotic sh3-domain protein that possibly acts as a bacterial ferrous iron-transport activating factor. | iron is vital to the majority of prokaryotes, with ferrous iron believed to be the preferred form for iron uptake owing to its much better solubility. the major route for bacterial ferrous iron uptake is found to be via an feo (ferrous iron-transport) system comprising the three proteins feoa, feob and feoc. although the structure and function of feob have received much attention recently, the roles played by feoa and feoc have been little investigated to date. here, the tertiary structure of fe ... | 2010 | 20516589 |
| aerosolized amikacin and oral clarithromycin to eradicate mycobacterium abscessus in a patient with cystic fibrosis: an 8-year follow-up. | 2010 | 20503290 | |
| screening for endophytic nitrogen-fixing bacteria in brazilian sugar cane varieties used in organic farming and description of stenotrophomonas pavanii sp. nov. | a gram-negative, rod-shaped, non-spore-forming and nitrogen-fixing bacterium, designated icb 89(t), was isolated from stems of a brazilian sugar cane variety widely used in organic farming. 16s rrna gene sequence analysis revealed that strain icb 89(t) belonged to the genus stenotrophomonas and was most closely related to stenotrophomonas maltophilia lmg 958(t), stenotrophomonas rhizophila lmg 22075(t), stenotrophomonas nitritireducens l2(t), [pseudomonas] geniculata atcc 19374(t), [pseudomonas] ... | 2011 | 20495025 |
| stenotrophomonas maltophilia flagellin induces a compartmentalized innate immune response in mouse lung. | intranasal (i.n.) instillation of different amounts of purified stenotrophomonas maltophilia flagellin preparation (1, 5 and 15 microg) in balb/c mice stimulated a transient innate immune response in the lungs. this was characterized by infiltration of different kinds of leukocytes (neutrophils, monocytes and lymphocytes), production of various inflammatory mediators (tumour necrosis factor alpha, interleukin 1 beta, interleukin 10, nitric oxide, myeloperoxidase and malondialdehyde) and activate ... | 2010 | 20488935 |
| degradation of microcystin-lr and rr by a stenotrophomonas sp. strain ems isolated from lake taihu, china. | a bacterial strain ems with the capability of degrading microcystins (mcs) was isolated from lake taihu, china. the bacterium was tentatively identified as a stenotrophomonas sp. the bacterium could completely consume mc-lr and mc-rr within 24 hours at a concentration of 0.7 microg/ml and 1.7 microg/ml, respectively. the degradation of mc-lr and mc-rr by ems occurred preferentially in an alkaline environment. in addition, mlra gene involved in the degradation of mc-lr and mc-rr was detected in e ... | 2010 | 20479990 |
| isolation and characterization of butachlor-catabolizing bacterial strain stenotrophomonas acidaminiphila js-1 from soil and assessment of its biodegradation potential. | isolation, characterization and assessment of butachlor-degrading potential of bacterial strain js-1 in soil. | 2010 | 20477958 |
| antimicrobial activity of the green tea polyphenol (-)-epigallocatechin-3-gallate (egcg) against clinical isolates of stenotrophomonas maltophilia. | stenotrophomonas maltophilia is increasingly recognised as an important nosocomial pathogen. treatment options are limited due to intrinsic resistance to many antibiotics as well as concerns over toxicity of the mainstay of treatment, co-trimoxazole. epigallocatechin-3-gallate (egcg), the major catechin found in green tea, has been shown to have antimicrobial effects against a number of bacterial pathogens. we evaluated the in vitro activity of this compound against 40 clinical isolates of s. ma ... | 2010 | 20472404 |
| comparison of e-test and disc diffusion methods for the in vitro evaluation of the antimicrobial activity of colistin in multi-drug resistant gram-negative bacilli. | to study susceptibility of clinical isolates of multi-drug resistant (mdr) gram-negative bacilli to colistin by minimum inhibitory concentration (mic) determination using etest, and compare this with their susceptibility measured by the disc diffusion (dd) method. | 2010 | 20464039 |
| genetic engineering of stenotrophomonas strain yc-1 to possess a broader substrate range for organophosphates. | in this work, stenotrophomonas sp. strain yc-1, a native soil bacterium that produces methyl parathion hydrolase (mph), was genetically engineered to possess a broader substrate range for organophosphates (ops). a surface anchor system derived from the truncated ice nucleation protein (inpnc) from pseudomonas syringae was used to target organophosphorus hydrolase (oph) onto the cell surface of strain yc-1, reducing the potential substrate uptake limitation. the surface localization of inpnc-oph ... | 2010 | 20455565 |
| [investigation of the in vitro antibacterial effects of statins]. | it was previously shown that statins have anti-inflammatory, immunomodulatory and anti-oxidant effects. this study was aimed to investigate the in-vitro antibacterial effects of simvastatin and atorvastatin. in this study antibacterial activity of the statins were tested against 16 methicillin-susceptible stophylococcus aureus (mssa), 16 methicillin-resistant s.aureus (mrsa), 16 methicillin-resistant coagulase-negative staphylococcus (mrcons), 9 vancomycin-susceptible enterococcus faecium, 7 van ... | 2010 | 20455414 |
| comparison of different antimicrobial susceptibility testing methods for stenotrophomonas maltophilia and results of synergy testing. | accurate determination of resistance is important to ensure appropriate antimicrobial therapy in stenotrophomonas maltophilia infections. this study was undertaken to evaluate the susceptibility results obtained by disc diffusion, e-test, phoenix system, and reference agar dilution method and also to evaluate the in vitro activity of various antimicrobial combinations against multidrug-resistant s. maltophilia. susceptibilities to several antimicrobial agents were determined by agar dilution, di ... | 2010 | 20449623 |
| chemical composition of endemic scorzonera sandrasica and studies on the antimicrobial activity against multiresistant bacteria. | the present study describes the antimicrobial activity and chemical composition of scorzonera sandrasica hartvig et strid (family asteraceae), endemic to turkey. the antimicrobial activity of the hexane, chloroform, ethyl acetate, and ethanol extracts of the aerial parts of s. sandrasica was evaluated against microorganisms, including multiresistant bacteria, using a paper disc diffusion method. the chemical composition of the chloroform extract of the plant was determined by gas chromatography ... | 2010 | 20438328 |
| colistin susceptibility testing: evaluation of reliability for cystic fibrosis isolates of pseudomonas aeruginosa and stenotrophomonas maltophilia. | antibiotic susceptibility methods that are commonly used to test bacterial isolates from patients with cystic fibrosis are of uncertain reliability for the polymyxins. to assess the reliability of four standard testing methods, this pilot study used a challenge set that included polymyxin-resistant isolates of pseudomonas aeruginosa and stenotrophomonas maltophilia. | 2010 | 20430789 |
| isolation and characterization of a virulent bacteriophage ab1 of acinetobacter baumannii. | acinetobacter baumannii is an emerging nosocomial pathogen worldwide with increasing prevalence of multi-drug and pan-drug resistance. a. baumannii exists widely in natural environment, especially in health care settings, and has been shown difficult to be eradicated. bacteriophages are often considered alternative agent for controlling bacterial infection and contamination. in this study, we described the isolation and characterization of one virulent bacteriophage ab1 capable of specifically i ... | 2010 | 20426877 |
| clinical characteristics of stenotrophomonas maltophilia infection in hematopoietic stem cell transplantation recipients: a single center experience. | recipients of hematopoietic stem-cell transplantation (hsct) are at high risk for infections caused by stenotrophomonas maltophilia. | 2010 | 20425134 |
| construction of river model biofilm for assessing pesticide effects. | due to the high importance of biofilms on river ecosystems, assessment of pesticides' adverse effects is necessary but is impaired by high variability and poor reproducibility of both natural biofilms and those developed in the laboratory. we constructed a model biofilm to evaluate the effects of pesticides, consisting in cultured microbial strains, pedobacter sp. 7-11, aquaspirillum sp. t-5, stenotrophomonas sp. 3-7, achnanthes minutissima n71, nitzschia palea n489, and/or cyclotella meneghinia ... | 2011 | 20422166 |
| structure, function, and insights into the biosynthesis of a head-to-head hydrocarbon in shewanella oneidensis strain mr-1. | a polyolefinic hydrocarbon was found in nonpolar extracts of shewanella oneidensis mr-1 and identified as 3,6,9,12,15,19,22,25,28-hentriacontanonaene (compound i) by mass spectrometry, chemical modification, and nuclear magnetic resonance spectroscopy. compound i was shown to be the product of a head-to-head fatty acid condensation biosynthetic pathway dependent on genes denoted as ole (for olefin biosynthesis). four ole genes were present in s. oneidensis mr-1. deletion of the entire oleabcd ge ... | 2010 | 20418444 |
| [identifying bacteria using a matrix-assisted laser desorption ionization time-of-flight (maldi-tof) mass spectrometer. comparison with routine methods used in clinical microbiology laboratories]. | the methods routinely used for bacterial identification in clinical microbiology laboratory, although miniaturized and automated, are still based on the same basic principles as classical identification methods. nevertheless, technological advances are emerging which could modify these routine methods. we report a comparative study between conventional identification methods and mass spectrometry maldi-tof (ms maldi-tof) for bacterial identification in the clinical microbiology laboratory. | 2010 | 20409613 |
| stenotrophomonas daejeonensis sp. nov., isolated from sewage. | a gram-stain-negative, motile, aerobic bacterial strain, designated mj03(t), was isolated from sewage and was characterized taxonomically by using a polyphasic approach. comparative 16s rrna gene sequence analysis showed that strain mj03(t) belongs to the family xanthomonadaceae, class gammaproteobacteria, and was related most closely to stenotrophomonas acidaminiphila amx 19(t) (97.9 % sequence similarity), stenotrophomonas humi r-32729(t) (97.1 %), stenotrophomonas nitritireducens l2(t) (96. ... | 2011 | 20400671 |
| introduction of an ampr-l2 intergenic segment attenuates the induced beta-lactamase activity of stenotrophomonas maltophilia. | expression of the l1 and l2 beta-lactamase genes is generally regulated by a lysr type regulator of the ampr in stenotrophomonas maltophilia. the ampr gene is located immediately upstream of l2 and is transcribed divergently, forming an ampr-l2 module. the ampr-l2 modules of 16 s. maltophilia isolates were analyzed, revealing that the ampr-l2 intergenic (ig) regions show a significant genetic diversity, whereas ampr proteins are highly conserved. the induction potential of the different ampr tow ... | 2010 | 20397035 |
| diagnostic value of pcr analysis of bacteria and fungi from blood in empiric-therapy-resistant febrile neutropenia. | this study aimed to assess the clinical utility of pcr for the analysis of bacteria and fungi from blood for the management of febrile neutropenic patients with hematologic malignancies. using a pcr system able to detect a broad range of bacteria and fungi, we conducted a prospective pilot study of periodic analyses of blood from patients following intensive chemotherapy. when fever occurred, it was treated with empirical antibiotic therapy, basically without knowledge of the pcr results. in 23 ... | 2010 | 20392911 |
| [complete nucleotide sequence of a plasmid pvae259 from vibrio alginolyticus and analysis of molecular biological characteristic of the plasmid]. | to obtain the complete nucleotide sequence of the plasmid pvae259 from vibrio alginolyticus, to analyze molecular characteristic of the plasmid, and to explore its potential function. | 2010 | 20387457 |
| ampn-ampg operon is essential for expression of l1 and l2 beta-lactamases in stenotrophomonas maltophilia. | ampg is an inner membrane permease which transports products of murein sacculus degradation from the periplasm into the cytosol in gram-negative bacteria. this process is linked to induction of the chromosomal ampc beta-lactamase gene in some members of the enterobacteriaceae and in pseudomonas aeruginosa. in this study, the ampg homologue of stenotrophomonas maltophilia kj was analyzed. the ampg homologue and its upstream ampn gene form an operon and are cotranscribed under the control of the p ... | 2010 | 20385866 |
| huanglongbing, a systemic disease, restructures the bacterial community associated with citrus roots. | to examine the effect of pathogens on the diversity and structure of plant-associated bacterial communities, we carried out a molecular analysis using citrus and huanglongbing as a host-disease model. 16s rrna gene clone library analysis of citrus roots revealed shifts in microbial diversity in response to pathogen infection. the clone library of the uninfected root samples has a majority of phylotypes showing similarity to well-known plant growth-promoting bacteria, including caulobacter, burkh ... | 2010 | 20382817 |
| adhesion to and biofilm formation on ib3-1 bronchial cells by stenotrophomonas maltophilia isolates from cystic fibrosis patients. | stenotrophomonas maltophilia has recently gained considerable attention as an important emerging pathogen in cystic fibrosis (cf) patients. however, the role of this microorganism in the pathophysiology of cf lung disease remains largely unexplored. in the present study for the first time we assessed the ability of s. maltophilia cf isolates to adhere to and form biofilm in experimental infection experiments using the cf-derived bronchial epithelial ib3-1cell line. the role of flagella on the ad ... | 2010 | 20374629 |
| stenotrophomonas maltophilia: a very rare cause of tropical pyomyositis. | pyomyositis is a purulent infection of skeletal muscles that arise from hematogenous spread associated with abscess formation. most often caused by staphylococcus aureus in more than 90-95% of cases but other rare organisms can cause this infection. herein we report a rare case of strenotrophomonas maltophilia as a cause of pyomyositis which is a rare occurrence, especially in immunocompetent adults. strenotrophomonas is a multidrug-resistant aerobic non-fermentative, non-sporulating, gram-negat ... | 2010 | 20374391 |
| antimicrobial susceptibilities of a worldwide collection of stenotrophomonas maltophilia isolates tested against tigecycline and agents commonly used for s. maltophilia infections. | antimicrobial susceptibilities were determined for 1,586 isolates of stenotrophomonas maltophilia from globally diverse medical centers using the clinical laboratory standards institute broth microdilution method. the combination trimethoprim-sulfamethoxazole (96.0% of isolates susceptible at < or =2 microg/ml trimethoprim and 38 microg/ml sulfamethoxazole) and tigecycline (95.5% of isolates sussceptible at < or =2 microg/ml) were the only antimicrobials tested with >94% susceptibility in all re ... | 2010 | 20368399 |
| complex bacterial infections pre- and posttransplant. | infections complications following lung transplantation are associated with significant morbidity and mortality. management of infections is most challenging in patients with cystic fibrosis (cf), but all lung transplant recipients are at heightened risk for opportunistic infections. particularly in cf, pretransplant infections with pseudomonas aeruginosa, other highly resistant bacteria (e.g., stenotrophomonas, burkholderia), and mycobacteria play a major role in recipient selection and post-lu ... | 2010 | 20354935 |
| identification of growth inhibition phenotypes induced by expression of bacterial type iii effectors in yeast. | many gram-negative pathogenic bacteria use a type iii secretion system to translocate a suite of effector proteins into the cytosol of host cells. within the cell, type iii effectors subvert host cellular processes to suppress immune responses and promote pathogen growth. numerous type iii effectors of plant and animal bacterial pathogens have been identified to date, yet only a few of them are well characterized. understanding the functions of these effectors has been undermined by a combinatio ... | 2010 | 20354502 |
| antibiotic resistance of stenotrophomonas maltophilia strains isolated from captive snakes. | the minimum inhibitory concentrations (mics) of 24 antibiotics were determined for 45 stenotrophomonas maltophilia strains by the microdilution method at 37 and 30 degrees c (after 24 h and 48 h of incubation). the isolates were obtained from mouth swabs and pus of 116 captive snakes whereas the identical strains (based on pfge) of the same origin were discarded. at 37 degrees c, the isolates showed a low frequency of resistance to levofloxacin (0 and 8.9 % of resistant strains after 24 and 48 h ... | 2010 | 20336510 |
| microbial contamination of suction tubes attached to suction instruments and preventive methods. | we investigated the microbial contamination of suction tubes attached to wall-type suction instruments. microbial contamination of suction tubes used for endoscopy or sputum suction in hospital wards was examined before and after their disinfection. in addition, disinfection and washing methods for suction tubes were evaluated. suction tubes (n=33) before disinfection were contaminated with 10(2)-10(8) colony-forming units (cfu)/tube. the main contaminants were pseudomonas aeruginosa, acinetobac ... | 2010 | 20332576 |
| role of excessive inflammatory response to stenotrophomonas maltophilia lung infection in dba/2 mice and implications for cystic fibrosis. | stenotrophomonas maltophilia is a pathogen that causes infections mainly in immunocompromised patients. despite increased s. maltophilia isolation from respiratory specimens of patients with cystic fibrosis (cf), the real contribution of the microorganism to cf pathogenesis still needs to be clarified. the aim of the present study was to evaluate the pathogenic role of s. maltophilia in cf patients by using a model of acute respiratory infection in dba/2 mice following a single exposure to aeros ... | 2010 | 20308302 |
| clinical characteristics of nosocomial infections in neonatal intensive care unit in eastern china. | a retrospective cohort study on nosocomial infections (ni) in the neonatal intensive care unit (nicu) was performed in the children's hospital of zhejiang university, hangzhou district, china. the most common infection site was pneumonia and bloodstream infection. low admission age, long nicu stay, and mechanical ventilation were significant risk factors for ni. klebsiella pneumoniae was the most common pathogen, followed by acinetobacter baumannii, staphylococcus epidermidi, pseudomonas aerugin ... | 2010 | 20297900 |
| changes in cystic fibrosis sputum microbiology in the united states between 1995 and 2008. | the study objective was to identify changes in cystic fibrosis (cf) sputum microbiology over 13 years. | 2010 | 20232473 |
| stenotrophomonas panacihumi sp. nov., isolated from soil of a ginseng field. | the study isolated a gram-negative, rod-shaped, non-motile bacterium from the soil of a ginseng field in daejeon, south korea and characterized it to determine its taxonomic position. phylogenetic analysis, based on the 16s rrna gene sequence, revealed that strain mk06(t) belongs to the family xanthomonadacea, and showed the highest degree of sequence similarity to stenotrophomonas rhizophila e-p10(t) (98.6%), xanthomonas campestris lmg 568t (98.0%), stenotrophomonas maltophilia atcc 1d3637(t) ( ... | 2010 | 20221726 |
| [microbial population of lower respiratory tract in children from different age groups with cystic fibrosis]. | to study microflora of lower respiratory tract of children from different age groups with cystic fibrosis during follow-up for determination of its variability and possible sources of infectious complications. | 2010 | 20218338 |
| reductive degradation of pyrazine-2-carboxylate by a newly isolated stenotrophomonas sp. hcu1. | a bacterium growing on pyrazine-2-carboxylate broth was isolated, purified and identified as a strain of stenotrophomonas sp. based on polyphasic taxonomic analyses and designated as strain hcu1. 16s rrna gene sequence of strain hcu1 showed 98.7% sequence similarity with the type strain of stenotrophomonas maltophilia belonging to gammaproteobacteria. growth of strain hcu1 was demonstrated when pyrazine-2-carboxylate was used as a sole source of nitrogen. ring reduction of pyrazine-2-carboxylate ... | 2010 | 20217461 |
| level of chemical and microbiological contaminations in chili bo (paste). | the objective of this study was to determine the level of preservatives and microbiological loads in various brands of commercially available chili bo (paste). fifteen different brands of chili bo obtained from the local market and hypermarkets were analyzed for ph, moisture and benzoic acid content, microbiological loads (aerobic, anaerobic, aerobic spores, and fungi), and thermophilic microorganisms. results showed that both moisture content and ph vary among samples. the concentrations of ben ... | 2010 | 20202342 |
| isolation of a methyl parathion-degrading strain stenotrophomonas sp. smsp-1 and cloning of the ophc2 gene. | a rod-shaped, gram-negative bacterium stenotrophomonas sp. smsp-1 was isolated from the sludge of a wastewater treating system of a pesticide manufacturer. strain smsp-1 could hydrolyze methyl parathion to p-nitrophenol (pnp) and dimethyl phosphorothioate but could not degrade pnp further. strain smsp-1 was able to hydrolyze other organophosphate pesticides, including fenitrothion, ethyl parathion, fenthion, and phoxim, but not chlorpyrifos. a 4395-bp dna fragment, including an organophosphorus ... | 2010 | 20182770 |
| risk factors for mortality among patients with stenotrophomonas maltophilia bacteremia in tokyo, japan, 1996-2009. | stenotrophomonas maltophilia is an important nosocomial pathogen, especially among immunocompromised patients. the objective of this study was to clarify the clinical characteristics, prognosis, and prognostic factors of patients with s. maltophilia bacteremia in japan. the microbiology records of all patients with s. maltophilia bacteremia between january 1996 and april 2009 at toranomon hospital, tokyo, japan, were retrospectively reviewed. a total of 53 cases of bacteremia were identified. th ... | 2010 | 20177726 |
| treatment of stenotrophomonas neonatal urinary tract infection with instillation of ciprofloxacin. | 2010 | 20165887 | |
| characterization of two diesel fuel degrading microbial consortia enriched from a non acclimated, complex source of microorganisms. | the bioremediation of soils impacted by diesel fuels is very often limited by the lack of indigenous microflora with the required broad substrate specificity. in such cases, the soil inoculation with cultures with the desired catabolic capabilities (bioaugmentation) is an essential option. the use of consortia of microorganisms obtained from rich sources of microbes (e.g., sludges, composts, manure) via enrichment (i.e., serial growth transfers) on the polluting hydrocarbons would provide biorem ... | 2010 | 20158909 |
| molecular identification by 16s rdna sequencing using excised corneal tissues: a useful diagnostic tool for refractory keratitis. | 2010 | 20151286 | |
| biotransformation of thianicotinyl neonicotinoid insecticides: diverse molecular substituents response to metabolism by bacterium stenotrophomonas maltophilia cgmcc 1.1788. | the carbon atom that neighbors the tertiary amine attached to the 6-chloro-3-pyridinylmethyl moiety is the key active site in the hydroxylation of the neonicotinoids imidacloprid and thiacloprid as well as in the demethylation of acetamiprid by stenotrophomonas maltophilia cgmcc 1.1788. in this study, thianicotinyl neonicotinoid insecticides having diverse molecular substituents were biotransformed by s. maltophilia cgmcc 1.1788. the results indicated that the substitution of 6-chloropyridyl in ... | 2010 | 20149644 |
| cyclobutanone analogues of beta-lactams revisited: insights into conformational requirements for inhibition of serine- and metallo-beta-lactamases. | the most important mode of bacterial resistance to beta-lactam antibiotics is the expression of beta-lactamases. new cyclobutanone analogues of penams and penems have been prepared and evaluated for inhibition of class a, b, c, and d beta-lactamases. inhibitors which favor conformations in which the c4 carboxylate is equatorial were found to be more potent than those in which the carboxylate is axial, and molecular modeling studies with enzyme-inhibitor complexes indicate that an equatorial orie ... | 2010 | 20141132 |
| molecular analysis of bacterial population structure and dynamics during cold storage of untreated and treated milk. | spoilage bacteria in milk are controlled by treatments such as thermization, microfiltration and addition of carbon dioxide. however, little information is known about the changes in microbial communities during subsequent cold storage of treated milk. culture-dependent methods and a direct molecular approach combining 16s rrna gene clone libraries and quantitative pcr (q-pcr) were applied to obtain a better overview of the structure and the dynamics of milk microbiota. raw milk samples were tre ... | 2010 | 20137820 |
| histamine, cadaverine, and putrescine produced in vitro by enterobacteriaceae and pseudomonadaceae isolated from spinach. | a total of 364 bacterial isolates, obtained from spinach leaves, were assayed in a decarboxylase broth containing histidine, lysine, and ornithine to check their ability to produce biogenic amines, and then quantified by high-performance liquid chromatography. among these isolates, 240 formed cadaverine, 208 formed putrescine, and 196 formed histamine, in widely varying amounts. they frequently produced more than one biogenic amine. klebsiella pneumoniae subsp. pneumoniae and morganella morganii ... | 2010 | 20132689 |
| colistin administration to pediatric and neonatal patients. | emergence of multidrug-resistant gram-negative nosocomial pathogens has led to resurgence of colistin use. safety and efficacy data regarding colistin use in pediatric patients are sparse, while optimal dosage has not been defined. we present a case series of neonates and children without cystic fibrosis treated with various doses of colistin intravenously. the records of patients who received colistin in a tertiary-care hospital from january 2007 to march 2009 were reviewed. thirteen patients ( ... | 2010 | 20119725 |
| isolation of uv-b resistant bacteria from two high altitude andean lakes (4,400 m) with saline and non saline conditions. | laguna (l.) negra and l. verde are high altitude andean lakes located at the 4,400 m altitude in the andean desert (puna) in the argentine northwest. both lakes are exposed to extreme weather conditions but differ in salinity contents (salinity 6.7% for l. negra and 0.27% for l. verde). the aim of this work was to isolate ultraviolet b fraction (uv-b) resistant bacteria under uv-stress in order to determine, a possible connection, between resistance to uv-b and tolerance to salinity. dna damage ... | 2009 | 20118609 |
| polymorphic mutation frequencies of clinical and environmental stenotrophomonas maltophilia populations. | mutation frequencies were studied in 174 stenotrophomonas maltophilia isolates from clinical and nonclinical environments by detecting spontaneous rifampin-resistant mutants in otherwise-susceptible populations. the distribution of mutation frequencies followed a pattern similar to that found for other bacterial species, with a modal value of 1 x 10(-8). nevertheless, the proportion of isolates showing mutation frequencies below the modal value (hypomutators) was significantly higher for s. malt ... | 2010 | 20097818 |
| [investigation of antibacterial activity of sertralin]. | sertralin is a psychotropic drug which acts by inhibiting the selective serotonin re-uptake in the synaptic area. previous studies have shown that some antidepressant agents have antibacterial activity. the aim of this study was to investigate the in vitro antibacterial activity of sertralin. a total of 224 bacterial strains isolated from clinical specimens together with standard control strains were included to the study. the antibacterial activity of sertralin was determined by microdilution m ... | 2009 | 20084919 |
| phylogenetic diversity of alkaline protease-producing psychrotrophic bacteria from glacier and cold environments of lahaul and spiti, india. | the diversity of proteolytic bacteria associated with a glacier and cold environment soils from three different locations in lahaul and spiti, india was investigated. two hundred seventeen bacterial strains were isolated in pure culture. subsequently these strains were screened for protease-production and one hundred nine showed protease production. from these protease producing psychrotrophic bacteria twenty showing high enzyme production at low temperature and alkaline ph were characterized an ... | 2010 | 20082368 |
| efficacy of copper-silver ionization in controlling biofilm- and plankton-associated waterborne pathogens. | the study was to determine the efficacy of copper-silver ionization against the formation of pseudomonas aeruginosa, stenotrophomonas maltophilia, and acinetobacter baumannii in biofilms and planktonic phases. at concentrations below the epa limits, ionization has potential to control the three waterborne pathogens, in addition to legionella, in hospital water systems for nosocomial infection control. | 2010 | 20080997 |
| novel variants of the smqnr family of quinolone resistance genes in clinical isolates of stenotrophomonas maltophilia. | recent analysis of stenotrophomonas maltophilia has identified a novel family of resistance genes (smqnr) encoding pentapeptide repeat proteins, which confer low-level resistance to quinolones. this study describes further novel variants present in clinical isolates of s. maltophilia and investigates their effect on resistance to a number of quinolones in an escherichia coli host. | 2010 | 20071366 |
| chronic mycobacterium abscessus infection and lung function decline in cystic fibrosis. | although nontuberculous mycobacteria (ntm) are recognized pathogens in cystic fibrosis (cf), associations with clinical outcomes remain unclear. | 2010 | 20071249 |
| colistin therapy for microbiologically documented multidrug-resistant gram-negative bacterial infections: a retrospective cohort study of 258 patients. | it is unclear whether the effectiveness of polymyxins depends on the site of infection, the responsible pathogen, dosage, and monotherapy vs. combination therapy. we investigated colistin therapy in a large, retrospective, single-centre, cohort study. primary analysis outcomes were infection outcome, survival and nephrotoxicity. over a 7-year period (october 2000 to october 2007), 258 patients received intravenous (i.v.) colistin for at least 72h for microbiologically documented multidrug-resist ... | 2010 | 20006471 |
| functional consortium for denitrifying sulfide removal process. | denitrifying sulfide removal (dsr) process simultaneously converts sulfide, nitrate, and chemical oxygen demand from industrial wastewaters to elemental sulfur, nitrogen gas, and carbon dioxide, respectively. this investigation utilizes a dilution-to-extinction approach at 10(-2) to 10(-6) dilutions to elucidate the correlation between the composition of the microbial community and the dsr performance. in the original suspension and in 10(-2) dilution, the strains stenotrophomonas sp., thauera s ... | 2010 | 19956940 |
| successful antibiotic lock therapy in patients with refractory peritonitis. | we describe two patients receiving peritoneal dialysis who experienced refractory peritonitis caused each by gram-negative bacteria: acinetobacter baumannii and stenotrophomonas maltophilia. these patients did not respond to appropriate intraperitoneal antibiotic therapy. we assumed bacterial colonization in the intra-abdominal portion of the catheter with biofilm. therefore, patients were treated with additional instillation of a ceftazidime-heparin lock into the catheter lumen after each excha ... | 2009 | 19954727 |
| clinical and histopathologic examination of ulcerating vocal fold lesions in an immunosuppressed patient. | we present the unique opportunity to correlate videostroboscopic findings with histologic examination. an immunocompromised patient with hoarseness because of ulcerative lesions of both vocal folds of uncertain cause died within a few weeks of initial presentation, and the larynx was donated for postmortem examination. relevant history, as well as endoscopic and histopathologic findings, is presented. | 2010 | 19944907 |
| epidemiology of bloodstream infections in patients with haematological malignancies with and without neutropenia. | all bacterial isolates from 7058 patients admitted to haemato-oncology wards at national taiwan university hospital between 2002 and 2006 were characterized. in total 1307 non-duplicate bloodstream isolates were made from all patients with haematological malignancy; 853 (65%) of these were from neutropenic patients. gram-negative bacteria predominated (60%) in neutropenic isolates with escherichia coli (12%), klebsiella pneumoniae (10%), acinetobacter calcoaceticus-baumannii complex (6%), and st ... | 2010 | 19941686 |
| novel tn4371-ice like element in ralstonia pickettii and genome mining for comparative elements. | integrative conjugative elements (ices) are important factors in the plasticity of microbial genomes. an element related to the ice tn4371 was discovered during a bioinformatic search of the ralstonia pickettii 12j genome. this element was analysed and further searches carried out for additional elements.a pcr method was designed to detect and characterise new elements of this type based on this scaffold and a culture collection of fifty-eight ralstonia pickettii and ralstonia insidiosa strains ... | 2009 | 19941653 |
| cold-active extracellular alkaline protease from an alkaliphilic stenotrophomonas maltophilia: production of enzyme and its industrial applications. | a novel psychro-tolerant bacterium stenotrophomonas maltophilia (mtcc 7528) with an ability to produce extracellular, cold-active, alkaline, and detergent-stable protease was isolated from soil samples obtained from gangotri glacier, western himalaya, india. the culture conditions for higher protease production were optimized with respect to incubation time, agitation, substrate, ph, and temperature. maximum protease production of 56.2 u x ml(-1) was achieved in the medium at 20 degrees c and ph ... | 2009 | 19940938 |
| 16s rrna gene-based identification of cultured bacterial flora from host-seeking ixodes ricinus, dermacentor reticulatus and haemaphysalis concinna ticks, vectors of vertebrate pathogens. | a total of 151 bacterial isolates were recovered from different developmental stages (larvae, nymphs and adults) of field-collected ticks (67 strains from ixodes ricinus, 38 from dermacentor reticulatus, 46 from haemaphysalis concinna). microorganisms were identified by means of 16s rrna gene sequencing. almost 87 % of the strains belonged to g(+) bacteria with predominantly occurring genera bacillus and paenibacillus. other g(+) strains included arthrobacter, corynebacterium, frigoribacterium, ... | 2009 | 19937215 |
| multidrug resistance in clinical isolates of stenotrophomonas maltophilia: roles of integrons, efflux pumps, phosphoglucomutase (spgm), and melanin and biofilm formation. | integrons, efflux pumps, phosphoglucomutase (spgm), and melanin and biofilm formation were investigated in 40 multidrug-resistant (mdr) and 30 non-mdr stenotrophomonas maltophilia isolates recovered from patients treated at national taiwan university hospital (taipei, taiwan). class 1 integrons were clearly associated with multidrug resistance. sequencing data revealed that aminoglycoside-modifying genes occurred most frequently in the integron and disclosed a new bla(imp-8)/aac6-ii/aada5 gene c ... | 2010 | 19926255 |