Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| botrytis fabiopsis, a new species causing chocolate spot of broad bean in central china. | the current study was conducted to identify botrytis spp. isolated from symptomatic broad bean plants grown in hubei province, china. among 184 botrytis strains, three distinct species, b. cinerea, b. fabae and a previously undescribed botrytis sp., were identified based on morphology of colonies, sclerotia and conidia. the novel botrytis sp. is described herein as a new species, botrytis fabiopsis sp. nov. at 20 c b. fabiopsis grew on potato dextrose agar (pda) at 12-13 mm d(-1), similar to b. ... | 2010 | 20943510 |
| arachidonic acid: an evolutionarily conserved signaling molecule modulates plant stress signaling networks. | fatty acid structure affects cellular activities through changes in membrane lipid composition and the generation of a diversity of bioactive derivatives. eicosapolyenoic acids are released into plants upon infection by oomycete pathogens, suggesting they may elicit plant defenses. we exploited transgenic arabidopsis thaliana plants (designated ep) producing eicosadienoic, eicosatrienoic, and arachidonic acid (aa), aimed at mimicking pathogen release of these compounds. we also examined their ef ... | 2010 | 20935246 |
| microfluidic immunosensor with micromagnetic beads coupled to carbon-based screen-printed electrodes (spces) for determination of botrytis cinerea in tissue of fruits. | a wide range of plant species, including economically important crops such as vegetables, ornamentals, bulbs, and fundamentally fruits, can be affected by gray mold caused by the fungal pathogen botrytis cinerea . this paper describes the development of a microfluidic immunosensor with micromagnetic beads (mmbs) coupled to carbon-based screen-printed electrodes (spces) for the rapid and sensitive quantification of b. cinerea in apple (red delicious), table grape (pink moscatel), and pear (willia ... | 2010 | 20931959 |
| [isolation and identification of an endophytic fungus of polygonatum cyrtonema and its antifungal metabolites]. | endophytic fungi in medicinal plants produce a variety of bioactivity compounds. in this study, an endophytic fungus zjqy610 with antifungal activity was isolated from polygonatum cyrtonema in zhejiang qingyuan baishanzu mountain nature reserve. | 2010 | 20931871 |
| synthesis, fungicidal activity, and structure-activity relationship of 2-oxo- and 2-hydroxycycloalkylsulfonamides. | to explore new potential fungicides, a series of novel compounds, including 11 2-oxocycloalkylsulfonamide (3) and 21 2-hydroxycycloalkylsulfonamide (4) derivatives, were synthesized and their structures were confirmed by (1)h nuclear magnetic resonance (nmr), infrared (ir), and elemental analysis. the results of the bioassay showed that the compounds 3 and 4 possessed excellent fungicidal activity against botrytis cinerea pers. both in vitro and in vivo. the fungicidal activity of the compounds ... | 2010 | 20929233 |
| the arabidopsis botrytis susceptible1 interactor defines a subclass of ring e3 ligases that regulate pathogen and stress responses. | we studied the function of arabidopsis (arabidopsis thaliana) botrytis susceptible1 interactor (boi) in plant responses to pathogen infection and abiotic stress. boi physically interacts with and ubiquitinates arabidopsis bos1, an r2r3myb transcription factor previously implicated in stress and pathogen responses. in transgenic plants expressing the bos1-β-glucuronidase transgene, β-glucuronidase activity could be detected only after inhibition of the proteosome, suggesting that bos1 is a target ... | 2010 | 20921156 |
| comparative analysis of defence responses induced by the endophytic plant growth-promoting rhizobacterium burkholderia phytofirmans strain psjn and the non-host bacterium pseudomonas syringae pv. pisi in grapevine cell suspensions. | plant growth-promoting rhizobacteria (pgpr) are beneficial microorganisms that colonize the rhizosphere of many plant species and confer beneficial effects, such as an increase in plant growth. pgpr are also well known as inducers of systemic resistance to pathogens in plants. however, the molecular mechanisms involved locally after direct perception of these bacteria by plant cells still remain largely unknown. burkholderia phytofirmans strain psjn is an endophytic pgpr that colonizes grapevine ... | 2010 | 20881012 |
| polyketide derivatives active against botrytis cinerea in gerbera hybrida. | a previously isolated cdna molecule from gerbera hybrida (asteraceae) codes for a new chalcone synthase-like polyketide synthase, 2-pyrone synthase (2ps). 2ps is able to synthesise 4-hydroxy-6-methyl-2-pyrone (triacetolactone), a putative precursor for gerberin and parasorboside, two abundant glucosides in gerbera. in this study, we show that gerbera plants transformed with the gene for 2ps in an antisense orientation and unable to synthesise gerberin and parasorboside are susceptible to botryti ... | 2010 | 20878179 |
| an attempt of postharvest orange fruit rot control using essential oils from mediterranean plants. | twelve essential oils from mediterranean aromatic plants were tested at different doses against four fungi known as causal agents of post-harvest orange fruit rot: botrytis cinerea, penicillium italicum, phytophthora citrophthora, and rhizopus stolonifer. essential oils were obtained from hyssopus officinalis, lavandula angustifolia, majorana hortensis, melissa officinalis, ocimum basilicum, origanum vulgare, salvia officinalis, and thymus vulgaris (family lamiaceae), verbena officinalis (family ... | 2010 | 20874226 |
| enantioselectivity of hydroxylation of racemic piperitone by fungi. | the biotransformation of racemic piperitone ((±)-1) was investigated using four strains of fungi selected in the screening procedure. the substrate was transformed by botrytis cinerea am235, absidia cylindrospora am336, absidia coerulea am93, and absidia glauca am177 into more polar metabolites. the transformation of racemic piperitone ((±)-1) catalyzed by b. cinerea am235 gave 7-hydroxypiperitone (2) as the only product. the biotransformation of (±)-1 by a. cylindrospora am336 afforded mixture ... | 2010 | 20872669 |
| use of a non-homologous end-joining-deficient strain (delta-ku70) of the biocontrol fungus trichoderma virens to investigate the function of the laccase gene lcc1 in sclerotia degradation. | the aim of this study was to apply a generated δtku70 strain with increased homologous recombination efficiency from the mycoparasitic fungus trichoderma virens for studying the involvement of laccases in the degradation of sclerotia of plant pathogenic fungi. inactivation of the non-homologous end-joining pathway has become a successful tool in filamentous fungi to overcome poor targeting efficiencies for genetic engineering. here, we applied this principle to the biocontrol fungus t. virens, s ... | 2010 | 20872221 |
| metallocene catalyzed synthesis of fungistatic vicinal aminoalcohols under solvent free conditions. | group 4 and 5 metallocenes, cp(2)ticl(2), cp(2)zrcl(2) and cp(2)vcl(2), have been evaluated as catalyst in the solvent free, room temperature, preparation of vicinal aminoalcohols. the regioselectivity of the reaction and the fungistatic activity of the prepared compounds against botrytis cinerea and colletotrichum gloeosporioides are discussed. | 2010 | 20864345 |
| the purple cauliflower arises from activation of a myb transcription factor. | anthocyanins are responsible for the color of many flowers, fruits, and vegetables. an interesting and unique purple (pr) gene mutation in cauliflower (brassica oleracea var botrytis) confers an abnormal pattern of anthocyanin accumulation, giving the striking mutant phenotype of intense purple color in curds and a few other tissues. to unravel the nature of the pr mutation in cauliflower, we isolated the pr gene via a combination of candidate gene analysis and fine mapping. pr encoded a r2r3 my ... | 2010 | 20855520 |
| propamidine decreas mitochondrial complex iii activity of botrytis cinerea. | propamidine, an aromatic diamidine compound, is widely used as an antimicrobial agent. to uncover its mechanism on pathogenetic fungi, botrytis cinerea as an object was used to investigate effects of propamidine in this paper. the transmission electron microscope results showed that the mitochondrial membranes were collapsed after propamidine treatment, followed that mitochondria were disrupted. inhibition of whole-cell and mitochondrial respiration by propamidine suggested that propamidine is m ... | 2010 | 20846494 |
| analysis of subgroup c of fungal chitinases containing chitin-binding and lysm modules in the mycoparasite trichoderma atroviride. | fungi have a plethora of chitinases, which can be phylogenetically divided into three subgroups (a, b and c). subgroup c (sgc) chitinases are especially interesting due to their multiple carbohydrate-binding modules, but they have not been investigated in detail yet. in this study, we analyzed sgc chitinases in the mycoparasites trichoderma atroviride and trichoderma virens. the expression of sgc chitinase genes in t. atroviride was induced during mycoparasitism of the fungal prey botrytis ciner ... | 2011 | 20843785 |
| involvement of lipoxygenase in elicitor-stimulated sanguinarine accumulation in papaver somniferum suspension cultures. | the involvement of lipoxygenase (lox, ec 1.13.11.12) in elicitor-induced opium poppy defense response was investigated. papaver somniferum l. suspension cultures were treated with abiotic elicitor methyl jasmonate (mj), fungal elicitor (botrytis cinerea homogenate) and phenidone (specific inhibitor of lox) to determine the involvement of this enzyme in production of sanguinarine, the major secondary metabolite of opium poppy cultures. p. somniferum suspension cultures responded to elicitor treat ... | 2010 | 20829053 |
| in vitro and in vivo antifungal activities of the essential oils of various plants against tomato grey mould disease agent botrytis cinerea. | the aim of this study was to find an alternative to synthetic fungicides currently used in the control of devastating fungal pathogen botrytis cinerea, the causal agent of grey mould disease of tomato. antifungal activities of essential oils obtained from aerial parts of aromatic plants, which belong to the lamiacea family such as origanum (origanum syriacum l. var. bevanii), lavender (lavandula stoechas l. var. stoechas) and rosemary (rosmarinus officinalis l.), were investigated against b. cin ... | 2010 | 20826038 |
| hypersensitivity pneumonitis due to molds in a saxophone player. | this 48-year-old patient was evaluated for an interstitial pneumonia. an open-lung biopsy showed a pattern of nonspecific interstitial pneumonia. the ct scan appearance, showing mosaic ground-glass opacities in the ventilated parts of the lung, the centrolobular predominance of inflammation on the lung sections, and the presence of a lymphocytic alveolitis at bal suggested a hypersensitivity pneumonitis. the patient was a white-collar worker and had no contact with pets, birds, drugs, or molds a ... | 2010 | 20822994 |
| arabidopsis histone methyltransferase set domain group8 mediates induction of the jasmonate/ethylene pathway genes in plant defense response to necrotrophic fungi. | as sessile organisms, plants have to endure a wide variety of biotic and abiotic stresses, and accordingly they have evolved intricate and rapidly inducible defense strategies associated with the activation of a battery of genes. among other mechanisms, changes in chromatin structure are thought to provide a flexible, global, and stable means for the regulation of gene transcription. in support of this idea, we demonstrate here that the arabidopsis (arabidopsis thaliana) histone methyltransferas ... | 2010 | 20810545 |
| characterization and pathogenicity of botryosphaeriaceae species collected from olive and other hosts in spain and california. | species in the family botryosphaeriaceae are common pathogens causing fruit rot and dieback of many woody plants. in this study, 150 botryosphaeriaceae isolates were collected from olive and other hosts in spain and california. representative isolates of each type were characterized based on morphological features and comparisons of dna sequence data of three regions: internal transcribed spacer 5.8s, β-tubulin, and elongation factor. three main species were identified as neofusicoccum mediterra ... | 2010 | 20731532 |
| sensitivity of botrytis cinerea to chitosan and acibenzolar-s-methyl. | the antifungal properties of chitosan and acibenzolar-s-methyl were evaluated to assess their potential for protecting grapes against botrytis cinerea pers.: fr. isolated from vitis vinifera l. the objectives were to determine the effects of these compounds on the in vitro development of b. cinerea and to assess their effectiveness at controlling grey mould on grapes stored at different temperatures. | 2010 | 20730989 |
| multiple resistance of botrytis cinerea from kiwifruit to sdhis, qois and fungicides of other chemical groups. | botrytis cinerea pers.: fr. is a high-risk pathogen for fungicide resistance development that has caused resistance problems on many crops throughout the world. this study investigated the fungicide sensitivity profile of isolates from kiwifruits originating from three greek locations with different fungicide use histories. sensitivity was measured by in vitro fungitoxicity tests on artificial nutrient media. | 2010 | 20730988 |
| functional characterization of three ethylene response factor genes from bupleurum kaoi indicates that bkerfs mediate resistance to botrytis cinerea. | three novel ethylene response factor (erf) genes, bkerf1, bkerf2.1 and bkerf2.2, were isolated from a medicinal plant, bupleurum kaoi. the deduced bkerfs contain a canonical nuclear localization signal and an erf/ap2 dna binding domain. rna gel blot analysis revealed that bkerf1 and bkerf2.1 were ubiquitously expressed at low levels in all parts of mature plants, and that bkerf2.2 was expressed at moderate levels in vegetative tissues. exogenous application of methyl jasmonate induced bkerf1/2.1 ... | 2011 | 20728241 |
| diverse accumulation of several dehydrin-like proteins in cauliflower (brassica oleracea var. botrytis), arabidopsis thaliana and yellow lupin (lupinus luteus) mitochondria under cold and heat stress. | dehydrins represent hydrophilic proteins acting mainly during cell dehydration and stress response. dehydrins are generally thermostable; however, the so-called dehydrin-like (dehydrin-related) proteins show variable thermolability. both groups immunoreact with antibodies directed against the k-segment of dehydrins. plant mitochondrial dehydrin-like proteins are poorly characterized. the purpose of this study was to extend previous reports on plant dehydrins by comparing the level of immunopreci ... | 2010 | 20718974 |
| abscisic acid deficiency causes changes in cuticle permeability and pectin composition that influence tomato resistance to botrytis cinerea. | a mutant of tomato (solanum lycopersicum) with reduced abscisic acid (aba) production (sitiens) exhibits increased resistance to the necrotrophic fungus botrytis cinerea. this resistance is correlated with a rapid and strong hydrogen peroxide-driven cell wall fortification response in epidermis cells that is absent in tomato with normal aba production. moreover, basal expression of defense genes is higher in the mutant compared with the wild-type tomato. given the importance of this fast respons ... | 2010 | 20709830 |
| trichome patterning in arabidopsis thaliana from genetic to molecular models. | the aerial organs of plants typically produce trichomes that may adopt various functions, including light, wind, frost, and herbivore protection. trichomes are of epidermal origin regularly distributed on the surface. the mechanism by which trichome differentiation is triggered in individual cells in a field of protodermal cells is best studied in arabidopsis thaliana. the genetic analysis has revealed a number of key genes controlling this patterning process, and further molecular analysis has ... | 2010 | 20705186 |
| induction of systemic resistance in plants by biochar, a soil-applied carbon sequestering agent. | biochar is the solid coproduct of biomass pyrolysis, a technique used for carbon-negative production of second-generation biofuels. the biochar can be applied as a soil amendment, where it permanently sequesters carbon from the atmosphere as well as improves soil tilth, nutrient retention, and crop productivity. in addition to its other benefits in soil, we found that soil-applied biochar induces systemic resistance to the foliar fungal pathogens botrytis cinerea (gray mold) and leveillula tauri ... | 2010 | 20701489 |
| postharvest management of gray mold and brown rot on surfaces of peaches and grapes using electrolyzed oxidizing water. | this study evaluated the potential use of near-neutral (ph=6.3-6.5) electrolyzed oxidizing water (eo water) to inactivate pure cultures of botrytis cinerea and monilinia fructicola and to mitigate fungal infection of these organisms on fruit surfaces. treatment of these organisms, in pure culture, with eo water at concentrations of 25, 50, 75, and 100ppm total residual chlorine (trc) and 10 min of contact time resulted in a 6 log(10) spores/ml reduction of both organisms. a dip treatment or a di ... | 2010 | 20696490 |
| inadvertent gene silencing of argininosuccinate synthase (bcass1) in botrytis cinerea by the plob1 vector system. | for several years, researchers working on the plant pathogen botrytis cinerea and a number of other related fungi have routinely used the plob1 vector system, based on hygromycin resistance, under the control of the aspergillus nidulans olic promoter and what was reported to be the beta-tubulin (tuba) terminator. recently, it has been demonstrated that this vector contains a 446-bp portion of the b. cinerea argininosuccinate synthase gene (bcass1) rather than the tuba terminator. as argininosucc ... | 2010 | 20696000 |
| exploring mechanisms of resistance to respiratory inhibitors in field strains of botrytis cinerea, the causal agent of gray mold. | respiratory inhibitors are among the fungicides most widely used for disease control on crops. most are strobilurins and carboxamides, inhibiting the cytochrome b of mitochondrial complex iii and the succinate dehydrogenase of mitochondrial complex ii, respectively. a few years after the approval of these inhibitors for use on grapevines, field isolates of botrytis cinerea, the causal agent of gray mold, resistant to one or both of these classes of fungicide were recovered in france and germany. ... | 2010 | 20693447 |
| distinct defenses induced in wheat against powdery mildew by acetylated and nonacetylated oligogalacturonides. | in wheat, little is known about disease resistance inducers and, more specifically, about the biological activities from those derived from endogenous elicitors, such as oligogalacturonides (ogas). therefore, we tested the ability of two fractions of ogas, with polymerization degrees (dps) of 2-25, to induce resistance to blumeria graminis f. sp. tritici and defense responses in wheat. one fraction was unacetylated (ogas-ac) whereas the second one was 30% chemically acetylated (ogas+ac). infecti ... | 2010 | 20684658 |
| genome characterization of a debilitation-associated mitovirus infecting the phytopathogenic fungus botrytis cinerea. | the full-length sequences of botrytiscinereamitovirus 1 (bcmv1) and an associated rna (bcmv1-s) in strain canbc-1c-78 of botrytis cinerea were determined. sequence analysis showed that bcmv1 is 2804 nt long and au-rich (66.8%). bcmv1 shares 95% nucleotide sequence identity with ophiostomanovo-ulmimitovirus 3b (onumv3b). however, it is 472 nt longer than onumv3b. mitochondrial codon usage revealed that bcmv1 contains one open reading frame encoding rdrp, which is 96% identical to the rdrp of onum ... | 2010 | 20674953 |
| disruption of the bcchs3a chitin synthase gene in botrytis cinerea is responsible for altered adhesion and overstimulation of host plant immunity. | the fungal cell wall is a dynamic structure that protects the cell from different environmental stresses suggesting that wall synthesizing enzymes are of great importance for fungal virulence. previously, we reported the isolation and characterization of a mutant in class iii chitin synthase, bcchs3a, in the phytopathogenic fungus botrytis cinerea. we demonstrated that virulence of this mutant is severely impaired. here, we describe the virulence phenotype of the cell-wall mutant bcchs3a on the ... | 2010 | 20672878 |
| antifungal activity of glyceollins isolated from soybean elicited with aspergillus sojae. | the aim of this study was to examine the efficacy of glyceollins isolated from soybean seeds elicited with aspergillus sojae for its antifungal potential. glyceollins i, ii, and iii were determined by hplc-ms analysis. the glyceollins (200 and 600 microg/disk) revealed remarkable antifungal effect against fusarium oxysporum , phytophthora capsici, sclerotinia sclerotiorum, and botrytis cinerea, within the growth inhibition range of 10.9-61.0%, along with their respective mic values ranging from ... | 2010 | 20666365 |
| mitogen-activated protein kinase 3 and 6 regulate botrytis cinerea-induced ethylene production in arabidopsis. | plants challenged by pathogens, especially necrotrophic fungi such as botrytis cinerea, produce high levels of ethylene. at present, the signaling pathways underlying the induction of ethylene after pathogen infection are largely unknown. mpk6, an arabidopsis stress-responsive mitogen-activated protein kinase (mapk) was previously shown to regulate the stability of acs2 and acs6, two type i acs isozymes (1-amino-cyclopropane-1-carboxylic acid synthase). phosphorylation of acs2 and acs6 by mpk6 p ... | 2010 | 20659280 |
| two new furanoeremophilanes from senecio santelisis. | from an argentine collection of senecio santelisis phil., the new furanoeremophilanoids, (10betah)-6beta-acetoxy-1alpha-hydroxyfuranoeremophilan-9-one (1) and (10betah)-1alpha-hydroxy-6beta-(propanoyloxy)furanoeremophilan-9-one (2), together with the known (10alphah)-6beta-acetoxy-1alpha-hydroxyfuranoeremophilan-9-one (3), (10alphah)-1alpha,6beta-diacetoxyfuranoeremophilan-9-one (4), and (10alphah)-1alpha-hydroxy-6beta-(propanoyloxy)furanoeremophilan-9-one (5) were isolated. their structures and ... | 2010 | 20658662 |
| microextraction and gas chromatography/mass spectrometry for improved analysis of geosmin and other fungal "off" volatiles in grape juice. | geosmin is a volatile fungal metabolite with an earthy aroma produced in grape products from rotten grapes. the accumulation of geosmin in grapes is caused by the interaction of botrytis cinerea and penicillium expansum. solid phase microextraction (spme) has great utility for collecting volatile compounds in wine. however, contamination with earthy odours may have occurred previously in the must and novel methods are required for this commodity. in the present report, several parameters of the ... | 2010 | 20655340 |
| antimicrobial activity of confertifolin from polygonum hydropiper. | confertifolin (6,6,9a-trimethyl-4,5,5a,6,7,8,9,9a-octahydronaphtho[1,2-c] furan-3 (1h)-one) was isolated from the essential oil of polygonum hydropiper l. (polygonaceae) leaves using column chromatography. confertifolin showed activity both in bacteria and fungi. the lowest mic for bacteria was observed against enterococcus faecalis (31.25 microg/ml). significant mic for fungi was observed against scopulariopsis sp (7.81 microg/ml), curvularia lunata (7.81 microg/ml), epidermophyton floccosum (7 ... | 2010 | 20645838 |
| uv-b impact on aphid performance mediated by plant quality and plant changes induced by aphids. | plants face various abiotic and biotic environmental factors and therefore need to adjust their phenotypic traits on several levels. uv-b radiation is believed to impact herbivorous insects via host plant changes. plant responses to abiotic challenges (uv-b radiation) and their interaction with two aphid species were explored in a multifactor approach. broccoli plants [brassica oleracea l. convar. botrytis (l.), brassicaceae] were grown in two differently covered greenhouses, transmitting either ... | 2010 | 20636911 |
| inhibitory activity of tea polyphenol and hanseniaspora uvarum against botrytis cinerea infections. | to investigate the effect of tea polyphenol (tp) and hanseniaspora uvarum alone or in combination against botrytis cinerea in grapes and to evaluate the possible mechanisms involved. | 2010 | 20633212 |
| antifungal activity of zinc oxide nanoparticles against botrytis cinerea and penicillium expansum. | antifungal activities of zinc oxide nanoparticles (zno nps) and their mode of action against two postharvest pathogenic fungi (botrytis cinerea and penicillium expansum) were investigated in this study. zno nps with sizes of 70 ± 15 nm and concentrations of 0, 3, 6 and 12 mmol l(-1) were used. traditional microbiological plating, scanning electron microscopy (sem), and raman spectroscopy were used to study antifungal activities of zno nps and to characterize the changes in morphology and cellula ... | 2011 | 20630731 |
| characters of aerated compost tea from immature compost that limit colonization of bean leaflets by botrytis cinerea. | the aim was to produce and characterize an aerated compost tea (act) that suppressed growth of the plant pathogen botrytis cinerea. | 2010 | 20629795 |
| mapk phosphatase mkp2 mediates disease responses in arabidopsis and functionally interacts with mpk3 and mpk6. | mitogen-activated protein kinase (mapk) cascades have important functions in plant stress responses and development and are key players in reactive oxygen species (ros) signalling and in innate immunity. in arabidopsis, the transmission of ros and pathogen signalling by mapks involves the coordinated activation of mpk6 and mpk3; however, the specificity of their negative regulation by phosphatases is not fully known. here, we present genetic analyses showing that mapk phosphatase 2 (mkp2) regula ... | 2010 | 20626661 |
| validation of a predictive model for the growth of botrytis cinerea and penicillium expansum on grape berries. | the objective of this study was to develop and to validate a model for predicting the combined effect of temperature and a(w) on the radial growth rate, mu, of botrytis cinerea and penicillium expansum on grape berries. the proposed strategy was based on the gamma-concept developed previously [zwietering, m.h., wijtzes, t., de wit, j.c., van't riet, k. 1992. a decision support system for prediction of the microbial spoilage in foods. journal of food protection. 12, 973-979]: mu=mu(opt).gamma(t). ... | 2010 | 20619474 |
| development of resistance against blackleg disease in brassica oleracea var. botrytis through in silico methods. | blackleg caused by leptosphaeria maculans is a very important disease worldwide and causes large yield loss of significant vegetable crops of brassicaceae family. absence of resistant 'b' genome in vegetable brassica oleracea proposed these species susceptible to blackleg disease. we present a specific piece of molecular modelling work, combining in silico docking methods, energy minimization calculations and in silico cloning, to develop a rational peptide as a candidate that functions as a res ... | 2010 | 20619351 |
| pesticides' influence on wine fermentation. | wine quality strongly depends on the grape quality. to obtain high-quality wines, it is necessary to process healthy grapes at the correct ripeness stage and for this reason the farmer has to be especially careful in the prevention of parasite attacks on the grapevine. the most common fungal diseases affecting grape quality are downy and powdery mildew (plasmopara viticola and uncinula necator), and gray mold (botrytis cinerea). on the other hand, the most dangerous insects are the grape moth (l ... | 2010 | 20610173 |
| a novel endophytic huperzine a-producing fungus, shiraia sp. slf14, isolated from huperzia serrata. | to characterize and identify a novel huperzine a (hupa)-producing fungal strain slf14 isolated from huperzia serrata (thunb. ex murray) trev. in china. | 2010 | 20602655 |
| evidence that core histone h3 is targeted to the mitochondria in brassica oleracea. | the core histone proteins h2a, h2b, h3 (histone h3) and h4 are known to form nucleosomes with nuclear dna, but are historically considered to be absent from mitochondria. we suggest that h3 is a dual-targeted protein, found in mitochondria as well as n (nuclei). wolf psort and mitoprot analyses of h3 sequences revealed mitochondrial targeting signals, and immunohistochemistry indicated mitochondrial distribution. western blots of brassica oleracea cv. botrytis (cauliflower) mitochondrial extract ... | 2010 | 20597863 |
| the mitogen-activated protein kinase kinase bos5 is involved in regulating vegetative differentiation and virulence in botrytis cinerea. | we present a characterization of bos5 from botrytis cinerea, a gene that encodes a mitogen-activated protein kinase kinase (mapkk), which is homologous to os-5 of neurospora crassa. the bos5 gene deletion mutant exhibited reduced vegetative growth and strongly impaired conidiation. the mutant also exhibited increased sensitivity to the dicarboximide fungicide iprodione and to osmotic stress mediated by nacl or kcl. western-blot analysis showed that the bcsak1 protein, the putative downstream com ... | 2010 | 20595070 |
| isolation and identification of 5-hydroxyl-5-methyl-2-hexenoic acid from actinoplanes sp. hbdn08 with antifungal activity. | a bioactivity-guided approach was employed to isolate and determine the chemical identity of bioactive constituents with antifungal activity from actinoplanes sp. hbdn08. the structure of the antifungal metabolite was elucidated as 5-hydroxyl-5-methyl-2-hexenoic acid on the basis of spectral analysis. this compound showed strong in vitro antifungal activity against botrytis cinerea, cladosporium cucumerinum and corynespora cassiicola, with an ic(50) of 32.45, 27.17, and 30.66 mg/l, respectively; ... | 2010 | 20584599 |
| potent cryoprotective activity of cold and co2-regulated cherimoya (annona cherimola) endochitinase. | a cryoprotective chitinase (bchi14) was isolated and purified from the mesocarp of co(2)-treated cherimoya fruit (annona cherimola mill.) stored at chilling temperature by anion exchange and chromatofocusing chromatography. this hydrolase was characterized as an endochitinase with a m(r) of 14.31 kda and a pi of 8.26, belonging to the family 19 of glycosyl hydrolases (gh19). while it was stable over a wide ph range and active in a broad acidic ph range, it had an optimum ph of 7.0. its optimum t ... | 2010 | 20576315 |
| biocatalytic preparation and absolute configuration of enantiomerically pure fungistatic anti-2-benzylindane derivatives. study of the detoxification mechanism by botrytis cinerea. | enantiomerically pure 2-benzylindane derivatives were prepared using biocatalytic methods and their absolute configuration determined. (1r,2s)-2-benzylindan-1-ol ((1r,2s)-2) and (s)-2-benzylindan-1-one ((s)-3) were produced by fermenting baker's yeast. lipase-mediated esterifications and hydrolysis of the corresponding racemic substrates gave rise to the enantiopure compounds (1s,2r)-2-benzylindan-1-ol ((1s,2r)-2) and (1r,2s)-2-benzylindan-1-ol ((1r,2s)-2), respectively. the antifungal activity ... | 2010 | 20574563 |
| cloning and characterization of a glutathione s-transferase homologue from the plant pathogenic fungus botrytis cinereadouble dagger. | abstract a gene was cloned from botrytis cinerea that encodes a protein homologous to glutathione s-transferase (gst). the gene, denominated bcgst1, is present in a single copy and represents the first example of such a gene from a filamentous fungus. the biochemical function of gsts is to conjugate toxic compounds to glutathione, thereby detoxifying the compound. in many other organisms, gst plays a role in chemical stress tolerance. we anticipated that gst functions for b. cinerea as a potenti ... | 2000 | 20572963 |
| the adenylate cyclase (bac) in botrytis cinerea is required for full pathogenicity. | summary the grey mould botrytis cinerea is an economically important plant pathogen. previously we found that null mutants of bcg1 encoding one of the two galpha subunits of heterotrimeric gtp-binding proteins differed in colony morphology and showed reduced pathogenicity. to further understand the mechanisms involved in infection, we cloned the bac gene encoding adenylate cyclase, the enzyme that catalyses production of camp from atp. the deduced protein sequence consists of 2300 amino acids, t ... | 2002 | 20569351 |
| t-dna tagging of a pathogen inducible promoter in arabidopsis thaliana. | summary many events associated with the plant defence responses are regulated on the transcriptional level. here we report the results of a promoter tagging approach to identify promoters that are induced upon pathogen attack in arabidopsis thaliana. a line was identified in a t-dna uida tagged arabidopsis library with induced gus expression after botrytis cinerea infection around the site of fungal infection. the upstream sequence was isolated and fused to the uida gene and tested in transgenic ... | 2002 | 20569331 |
| functional analysis of an extracellular catalase of botrytis cinerea. | summary there is evidence that the necrotrophic fungal pathogen botrytis cinerea is exposed to oxidative processes within plant tissues. the pathogen itself also generates active oxygen species and h(2)o(2) as pathogenicity factors. our aim was to study how the pathogen may defend itself against cellular damage caused by the accumulation of h(2)o(2) and the role of an extracellular catalase in its detoxification during the infection of tomato and bean plants by b. cinerea. chloronaphthol stainin ... | 2002 | 20569330 |
| resistance to fungal pathogens triggered by the cf9-avr9 response in tomato and oilseed rape in the absence of hypersensitive cell death. | summary in tomato and related species, the cf9 resistance gene induces hypersensitive cell death and activates downstream defence pathways upon recognition of the avr9 elicitor. we investigated whether the cf9-avr9 response without hypersensitive cell death symptoms increases resistance to several fungi. a low avr9 dose that does not cause hypersensitive cell death was injected in cf9 tomato and transgenic cf9 oilseed rape plants. subsequently, the injected leaves were infected with different fu ... | 2002 | 20569306 |
| functional analysis of botrytis cinerea pectin methylesterase genes by pcr-based targeted mutagenesis: bcpme1 and bcpme2 are dispensable for virulence of strain b05.10. | summary botrytis cinerea is a necrotrophic pathogen that produces an array of enzymes capable of attacking the plant cell wall components. we have previously shown that growth of the fungus in planta is accompanied by the degradation of pectin and that endopolygalacturonase (bcpg) genes are expressed during infection of different plant tissues. it was assumed that pectin demethylation by pectin methylesterases (pme) was essential for the subsequent depolymerization by bcpgs to occur efficiently. ... | 2005 | 20565686 |
| molecular and functional characterization of a secreted lipase from botrytis cinerea. | summary a previous study has indicated that a cutinolytic lipase from botrytis cinerea was required for penetration of an intact plant host cuticle and infection (comménil et al., 1998, physiol. mol. plant pathol. 52, 1-14). in order to clarify the role of this lipase, the corresponding gene (lip1) was cloned. in vitro, the lip1-encoded lipase was inducibly expressed and subject to catabolite repression. on the leaf surface, the cuticle served as an inducer. lip1 knock-out mutants lacked lipase ... | 2005 | 20565655 |
| a novel seven-helix transmembrane protein btp1 of botrytis cinerea controls the expression of gst-encoding genes, but is not essential for pathogenicity. | summary to gain new insights into the signalling mechanisms of the grey mould botrytis cinerea, which causes several pre- and post-harvest diseases on a variety of host plants, we cloned, sequenced and functionally characterized a gene, btp1, encoding a novel 391-amino acid transmembrane protein. the protein btp1 shows similarity to the transmembrane protein pth11, which is essential for appressorium formation and successful colonization of plant tissue in the rice blast fungus magnaporthe grise ... | 2005 | 20565654 |
| determinants of pseudomonas putida wcs358 involved in inducing systemic resistance in plants. | summary pseudomonas putida wcs358 is a plant growth-promoting rhizobacterium originally isolated from the rhizosphere of potato. it can suppress soil-borne plant diseases by siderophore-mediated competition for iron, but it has also been reported to result in induced systemic resistance (isr) in arabidopsis thaliana. bacterial determinants of this strain involved in inducing systemic resistance in arabidopsis were investigated using a tn5 transposon mutant defective in biosynthesis of the fluore ... | 2005 | 20565648 |
| evaluation of tolerance to pierce's disease and botrytis in transgenic plants of vitis vinifera l. expressing the pear pgip gene. | summary polygalacturonase-inhibiting proteins (pgips) are plant cell-wall proteins that specifically inhibit fungal endo-polygalacturonases (pgs) that contribute to the aggressive decomposition of susceptible plant tissues. the inhibition of fungal pgs by pgips suggests that pgips have a role in plant tolerance to fungal infections and this has been observed in transgenic plants expressing pgips. xylella fastidiosa, the causal agent of pierce's disease (pd) in grapevines, has genes that encode c ... | 2005 | 20565637 |
| a constitutive pr-1::luciferase expression screen identifies arabidopsis mutants with differential disease resistance to both biotrophic and necrotrophic pathogens. | summary a complex signal transduction network involving salicylic acid, jasmonic acid and ethylene underlies disease resistance in arabidopsis. to understand this defence signalling network further, we identified mutants that expressed the marker gene pr-1::luciferase in the absence of pathogen infection. these cir mutants all display constitutive expression of a suite of defence-related genes but exhibit different disease resistance profiles to two biotrophic pathogens, pseudomonas syringae pv. ... | 2005 | 20565636 |
| induction of programmed cell death in lily by the fungal pathogen botrytis elliptica. | summary the genus botrytis contains necrotrophic plant pathogens that have a wide host range (b. cinerea) or are specialized on a single host species, e.g. b. elliptica on lily. in this study, it was found that b. elliptica-induced cell death of lily displays hallmark features of animal programmed cell death or apoptosis including cytoplasmic shrinkage, nuclear dna fragmentation and the accumulation of no as well as h(2)o(2). a pharmacological approach showed that b. elliptica-induced cell death ... | 2004 | 20565630 |
| the jasmonate-insensitive mutant jin1 shows increased resistance to biotrophic as well as necrotrophic pathogens. | summary jasmonic acid and related oxylipin compounds are plant signalling molecules that are involved in the response to pathogens, insects, wounding and ozone. to explore further the role of jasmonates in stress signal transduction, the response of two jasmonate-signalling mutants, jin1 and jin4, to pathogens and ozone was analysed in this study. upon treatment with the biotrophic bacterial pathogen pseudomonas syringae, endogenous jasmonate levels increased in jin1 and jin4 similar to wild-typ ... | 2004 | 20565618 |
| functional analysis of h(2)o(2)-generating systems in botrytis cinerea: the major cu-zn-superoxide dismutase (bcsod1) contributes to virulence on french bean, whereas a glucose oxidase (bcgod1) is dispensable. | summary the oxidative burst, a transient and rapid accumulation of reactive oxygen species (ros), is a widespread defence mechanism of higher plants against pathogen attack. there is increasing evidence that the necrotrophic fungal pathogen botrytis cinerea itself generates ros, and that this capability could contribute to the virulence of the fungus. two potential h(2)o(2)-generating systems were studied with respect to their impact on the interaction of b. cinerea and its host plant phaseolus ... | 2004 | 20565578 |
| the botrytis cinerea early secretome. | the extracellular proteome, or secretome, of phytopathogenic fungi is presumed to be a key element of their infection strategy. especially interesting constituents of this set are those proteins secreted at the beginning of the infection, during the germination of conidia on the plant surfaces or wounds, since they may play essential roles in the establishment of a successful infection. we have germinated botrytis cinerea conidia in conditions that resemble the plant environment, a synthetic med ... | 2010 | 20564262 |
| arabidopsis thaliana plant defensin atpdf1.1 is involved in the plant response to biotic stress. | *previously, it was shown that the arabidopsis thaliana plant defensins atpdf1.1 (at1g75830) and atpdf1.2a (at5g44420) exert in vitro antimicrobial properties and that their corresponding genes are expressed in seeds and induced in leaves upon pathogen attack, respectively. *in this study, the expression profile of both atpdf1.1 and atpdf1.2a is analysed in wild-type plants upon different stress-related treatments and the effect of modulation of their expression in transgenic plants is examined ... | 2010 | 20561213 |
| a copper-transporting atpase bcccc2 is necessary for pathogenicity of botrytis cinerea. | copper is an essential trace element that serves as a cofactor for numerous enzymes. in eukaryotes, copper-transporting atpases deliver copper to various copper-containing proteins in the trans-golgi network. this study identified a copper-transporting atpase gene bcccc2 in a fungus pathogenic to plants, botrytis cinerea. we investigated the biological roles of bcccc2 by generating null mutants for bcccc2. melanization, conidiation and the formation of sclerotia were severely affected in deltabc ... | 2010 | 20526618 |
| [the effect of different endophytic fungi on chrysanthemum morifolium output and quality]. | to enhance the flowers yield of chrysanthemum morifolium by endophytic fungi. | 2010 | 20518295 |
| methodological improvements in the expression of foreign genes and in gene replacement in the phytopathogenic fungus botrytis cinerea. | summary genetic transformation is generally carried out in botrytis cinerea by random integration of the foreign dna into the genome, resulting in transformants that show differences among them in, for example, the expression of a reporter gene. here we report a system for site-directed integration in which a novel recipient strain containing a 5'-truncated copy of the hygromycin resistance gene, hph, is transformed with a vector containing another truncated copy, now in the opposite end, of the ... | 2007 | 20507541 |
| botrytis cinerea: the cause of grey mould disease. | introduction: botrytis cinerea (teleomorph: botryotinia fuckeliana) is an airborne plant pathogen with a necrotrophic lifestyle attacking over 200 crop hosts worldwide. although there are fungicides for its control, many classes of fungicides have failed due to its genetic plasticity. it has become an important model for molecular study of necrotrophic fungi. taxonomy: kingdom: fungi, phylum: ascomycota, subphylum: pezizomycotina, class: leotiomycetes, order: helotiales, family: sclerotiniaceae, ... | 2007 | 20507522 |
| functional analysis of nlp genes from botrytis elliptica. | summary we functionally analysed two nep1-like protein (nlp) genes from botrytis elliptica (a specialized pathogen of lily), encoding proteins homologous to the necrosis and ethylene-inducing protein (nep1) from fusarium oxysporum. single gene replacement mutants were made for benep1 and benep2, providing the first example of transformation and successful targeted mutagenesis in this fungus. the virulence of both mutants on lily leaves was not affected. benep1 and benep2 were individually expres ... | 2007 | 20507492 |
| the slt2-type map kinase bmp3 of botrytis cinerea is required for normal saprotrophic growth, conidiation, plant surface sensing and host tissue colonization. | summary mitogen-activated protein kinases (mapks) play important roles in signal transduction and regulation of various aspects of saprotrophic and pathogenic growth in plant pathogenic fungi. we have generated a botrytis cinerea knock-out mutant in the bmp3 gene encoding a homologue of the yeast slt2 cell wall integrity mapk. the deltabmp3 mutant showed reduced vegetative growth on various media, strongly impaired conidiation and loss of sclerotia formation. growth retardation of the mutant was ... | 2007 | 20507489 |
| histochemical and genetic analysis of host and non-host interactions of arabidopsis with three botrytis species: an important role for cell death control. | summary susceptibility was evaluated of host and non-host plants to three pathogenic botrytis species: the generalist b. cinerea and the specialists b. elliptica (lily) and b. tulipae (tulip). b. tulipae was, unexpectedly, able to infect plant species other than tulip, and to a similar extent as b. cinerea. to study host and non-host interactions in more detail, the three botrytis species were inoculated on arabidopsis wild-types and 23 mutant genotypes. disease development was monitored macrosc ... | 2007 | 20507477 |
| expression of barley bax inhibitor-1 in carrots confers resistance to botrytis cinerea. | summary bax inhibitor-1 (bi-1) is a protein that controls heterologous bax-induced cell death, the hypersensitive reaction and abiotic stress-induced cell death in plants. when over-expressed in epidermal cells of barley, barley bi-1 induces susceptibility to the biotrophic fungal pathogen blumeria graminis. when we expressed barley bi-1 in carrot susceptible to the necrotrophic fungus botrytis cinerea, we obtained bi-1-mediated resistance to fungus-induced leaf cell death and less fungal spread ... | 2006 | 20507447 |
| enhancement of biocontrol efficacy of rhodotorula glutinis by salicyclic acid against gray mold spoilage of strawberries. | the control activity of rhodotorulaglutinis, salicylic acid (sa), alone or in combination, on gray mold spoilage and natural spoilage of strawberries was investigated. r. glutinis as stand-alone treatment, and the combined treatment of sa at 100 microg/ml with r. glutinis significantly reduced the disease incidence and lesion diameter of gray mold spoilage, respectively at both 20 degrees c and 4 degrees c. after 20 d storage at 4 degrees c, the combination of sa and r. glutinis was more effecti ... | 2010 | 20488570 |
| outdoor allergenic fungal spores: comparison between an urban and a rural area in northern portugal. | the frequency and concentration of many airborne fungal spores associated with respiratory allergy symptoms are influenced by geographical and climatic characteristics. | 2010 | 20461966 |
| antifungal activities of an endophytic pseudomonas fluorescens strain pf1tz harbouring genes from pyoluteorin and phenazine clusters. | pseudomonas fluorescens pf1tz was inhibitory in vitro to a number of phytopathogenic fungi and could protect vine plantlets against botrytis cinerea. total protection was reached after 3 weeks of bacterial inoculation. the endophytism of pf1tz was confirmed by confocal microscopy using its inherent fluorescence. the molecular characterization of pf1tz indicated the presence of genes from clusters encoding pyoluteorin and phenazine. the chromatographic purification and lc-ms(n) analysis revealed ... | 2010 | 20458521 |
| synthesis of nh006--a photostable fungicide effective against botrytis cinerea--according to the asymmetric total synthesis of mk8383. | mk8383, isolated from phoma sp. t2526 in 1993, exhibits potent antibiotic activities against a variety of phytopathogens and has been considered a promising fungicide against botrytis cinerea. unfortunately, mk8383 is a photosensitive compound and it undergoes irreversible decomposition. although much effort has been devoted to improving the photostability of mk8383 by chemical modification of its structure by a research group organized by meiji seika kaishya, ltd. and mitsubishi chemical corpor ... | 2010 | 20449485 |
| aeromycological profile of indoor and outdoor environments. | the aim of this work was to determine the differences between indoor and outdoor aeromicological composition. the aerobiological study was performed, from 15 january to 14 april 2008, using two volumetric spore traps, one placed indoors and another positioned outdoors on the roof of the faculdade de ciências building. a total of 23 000 spores were sampled outdoors and 15 500 spores were identified indoors. in both environments, the most abundant fungal spores were cladosporium, aspergillus/penic ... | 2009 | 20449225 |
| role of nitric oxide and reactive oxide species in disease resistance to necrotrophic pathogens. | nitric oxide (no) and reactive oxygen species (ros) are important signaling molecules in plant immunity. however, roles of no and ros in disease resistance to necrotrophic pathogens are not fully understood. we have recently demonstrated that no plays a pivotal role in basal defense against botrytis cinerea and the expression of the salicylic acid (sa) -responsive gene pr-1in nicotiana benthamiana. by contrast, ros function negatively in resistance or positively in expansion of disease lesions d ... | 2010 | 20448455 |
| a role for beta-sitosterol to stigmasterol conversion in plant-pathogen interactions. | upon inoculation with pathogenic microbes, plants induce an array of metabolic changes that potentially contribute to induced resistance or even enhance susceptibility. when analysing leaf lipid composition during the arabidopsis thaliana-pseudomonas syringae interaction, we found that accumulation of the phytosterol stigmasterol is a significant plant metabolic process that occurs upon bacterial leaf infection. stigmasterol is synthesized from beta-sitosterol by the cytochrome p450 cyp710a1 via ... | 2010 | 20444228 |
| a domain swap approach reveals a role of the plant wall-associated kinase 1 (wak1) as a receptor of oligogalacturonides. | oligogalacturonides (ogs) released from the plant cell wall are active both as damage-associated molecular patterns (damps) for the activation of the plant immune response and regulators of plant growth and development. members of the wall-associated kinase (wak) family are candidate receptors of ogs, due to their ability to bind in vitro these oligosaccharides. because lethality and redundancy have hampered the study of waks by reverse genetics, we have adopted a chimeric receptor approach to e ... | 2010 | 20439716 |
| the response of antioxidant metabolism in apple fruit to post-harvest storage disease. | 2009 | 20420239 | |
| deficiencies in jasmonate-mediated plant defense reveal quantitative variation in botrytis cinerea pathogenesis. | despite the described central role of jasmonate signaling in plant defense against necrotrophic pathogens, the existence of intraspecific variation in pathogen capacity to activate or evade plant jasmonate-mediated defenses is rarely considered. experimental infection of jasmonate-deficient and jasmonate-insensitive arabidopsis thaliana with diverse isolates of the necrotrophic fungal pathogen botrytis cinerea revealed pathogen variation for virulence inhibition by jasmonate-mediated plant defen ... | 2010 | 20419157 |
| improved tolerance toward fungal diseases in transgenic cavendish banana (musa spp. aaa group) cv. grand nain. | the most devastating disease currently threatening to destroy the banana industry worldwide is undoubtedly sigatoka leaf spot disease caused by mycosphaerella fijiensis. in this study, we developed a transformation system for banana and expressed the endochitinase gene then-42 from trichoderma harzianum together with the grape stilbene synthase (stsy) gene in transgenic banana plants under the control of the 35s promoter and the inducible pr-10 promoter, respectively. the superoxide dismutase ge ... | 2011 | 20397044 |
| [construction of t-dna insertion mutants of botrytis cinerea via agrobacterium tumefaciens mediated transformation and sequence analysis of insertion site]. | construction of botrytis cinerea t-dna insertion mutant library through agrobacterium-mediated transformation and for further understanding of the molecular mechanisms underlying the pathogenicity of botrytis cinerea. | 2010 | 20387458 |
| transcriptional responses to polycyclic aromatic hydrocarbon-induced stress in arabidopsis thaliana reveal the involvement of hormone and defense signaling pathways. | polycyclic aromatic hydrocarbons (pahs) are toxic, widely-distributed, environmentally persistent, and carcinogenic byproducts of carbon-based fuel combustion. previously, plant studies have shown that pahs induce oxidative stress, reduce growth, and cause leaf deformation as well as tissue necrosis. to understand the transcriptional changes that occur during these processes, we performed microarray experiments on arabidopsis thaliana l. under phenanthrene treatment, and compared the results to ... | 2010 | 20377843 |
| 2-de proteomic approach to the botrytis cinerea secretome induced with different carbon sources and plant-based elicitors. | botrytis cinerea is a phytopathogenic fungus infecting a number of crops (tomatoes, grapes and strawberries), which has been adopted as a model system in molecular phytopathology. b. cinerea uses a wide variety of infection strategies, which are mediated by a set of genes/proteins called pathogenicity/virulence factors. many of these factors have been described as secreted proteins, and thus the study of this sub-proteome, the secretome, under changing circumstances can help us to understand the ... | 2010 | 20376862 |
| wounding-induced wrky8 is involved in basal defense in arabidopsis. | the wrky family of plant transcription factors controls several types of plant stress responses. arabidopsis wrky8, localized to the nucleus, is mainly induced by abscissic acid, h(2)o(2), wounding, pseudomonas syringae and botrytis cinerea infection, and aphid and maggot feeding. to determine its biological functions, we isolated loss-of-function t-dna insertion mutants and generated gain-of-function overexpressing wrky8 transgenic plants in arabidopsis. plants expressing the mutated wrky8 gene ... | 2010 | 20367464 |
| ulocladium cantlous sp. nov. isolated from northwestern china: its morphology and molecular phylogenetic position. | a new species of ulocladium was isolated from diseased leaves from two cucumis sp. growing in sinkiang and gansu provinces of china. conidia were isolated from necrotic leaves and used to establish single-spore pure cultures. conidia were harvested from cultures 7 d after incubation for morphological comparisons. the morphology of this species resembles that of u. botrytis and u. consortiale. however it is distinguished from these two species by the sizes of obovoid to broadly ellipsoidal conidi ... | 2010 | 20361504 |
| the arabidopsis p450 protein cyp82c2 modulates jasmonate-induced root growth inhibition, defense gene expression and indole glucosinolate biosynthesis. | jasmonic acid (ja) is a fatty acid-derived signaling molecule that regulates a broad range of plant defense responses against herbivores and some microbial pathogens. molecular genetic studies have established that ja also performs a critical role in several aspects of plant development. here, we describe the characterization of the arabidopsis mutant jasmonic acid-hypersensitive1-1 (jah1-1), which is defective in several aspects of ja responses. although the mutant exhibits increased sensitivit ... | 2010 | 20354503 |
| contamination by moulds of grape berries in slovakia. | this paper describes the first map, albeit partial, of toxigenic fungi re-isolated from grape berries collected in three out of the six most important slovakia winemaking areas in two different periods of the harvest year 2008. low temperatures and high relative humidity during july 2008 favoured the development of grape fungal diseases that cause rots such as plasmopara, uncinula, botrytis, metasphaeria, elsinoë, and saccharomycetes. in the analysed samples, the following genera of toxigenic fu ... | 2010 | 20349371 |
| genetic characterization of grapevine-infecting botrytis cinerea isolates from argentina. | botrytis cinerea is an ascomycete with a high genetic diversity and complex population structure, as reported from several hosts and sites. however, nothing is known about its genetic diversity in argentina. | 2010 | 20346300 |
| insights into the mechanisms of toxicity and tolerance to the agricultural fungicide mancozeb in yeast, as suggested by a chemogenomic approach. | abstract saccharomyces cerevisiae was used to uncover the mechanisms underlying tolerance and toxicity of the agricultural fungicide mancozeb, linked to cancer and parkinson's disease development. chemogenomics screening of a yeast deletion mutant collection revealed 286 genes that provide protection against mancozeb toxicity. the most significant gene ontology (go) terms enriched in this dataset are associated to transcriptional machinery, vacuolar organization and biogenesis, intracellular tra ... | 2010 | 20337531 |
| oligomycins a and c, major secondary metabolites isolated from the newly isolated strain streptomyces diastaticus. | during the screening program for fungicides, one actinomycete strain eco 00047 was isolated with the potential activity against fungus. according to the morphology and analysis of the nucleotide sequence of the 16s rrna gene (1500 bp) this isolate was identified as streptomyces diastaticus. the active compounds were separated by silica gel column chromatography, sephadex lh-20 gel filtration and then purified by flash chromatography on c18 (20-45 microm). the chemical structure of the bioactive ... | 2010 | 20336498 |
| occurrence of botrytis fabae sardiña in england. | 1947 | 20256153 | |
| analysis of fungal gene expression by real time quantitative pcr. | the real-time quantitative pcr (qpcr) method has become central for the quantification of gene expression as well as other applications. the major advantages of qpcr are the utilization of small amount of template, high sensitivity and the ability to detect products during the reaction. after selecting qpcr among other options (northern blot, semi-quantitative pcr), one should consider several factors. the first and critical step in qpcr of fungi is the selection of an appropriate growth medium ... | 2010 | 20238263 |
| effect of culture media and ph on the biomass production and biocontrol efficacy of a metschnikowia pulcherrima strain to be used as a biofungicide for postharvest disease control. | few strains of metschnikowia pulcherrima (pitt) m.w. miller are under development for control of postharvest pathogens on fruit. a substrate was developed to optimize the biomass production of m. pulcherrima strain bio126. different complex nutrient sources, with or without ph control, were tested. growth in yeast extract provided at concentrations > or =30 g*l-1 yielded the highest biomass. the addition of two carbon sources, d-mannitol and l-sorbose, at 5 g*l-1 each, significantly improved yea ... | 2010 | 20237574 |