Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| epidemiological, diagnostic, clinical, and therapeutic aspects of brucella bacteremia in children in southern israel: a 7-year retrospective study (2005-2011). | data on the various aspects of brucellosis in children living in southern israel are missing. | 2015 | 25793475 |
| the prevalence and distribution of brucella melitensis in goats in malaysia from 2000 to 2009. | a study was conducted to describe the prevalence and distribution of zoonotic brucella melitensis in goats in peninsular malaysia. using serosurveillance data of the last decade (2000-2009) involving 119,799 goats and 3555 farms, the seroprevalence of brucellosis among goats was 0.91% (95% ci=0.86-0.96) and among farms was 7.09% (95% ci=6.27-7.98). the odds of brucellosis was significantly (p<0.05) higher in the later part of the decade, in larger herd size and among the states located in the pe ... | 2015 | 25746928 |
| vaccination with recombinant l7/l12-truncated omp31 protein induces protection against brucella infection in balb/c mice. | brucellosis is the most common bacterial zoonotic disease worldwide and no vaccine is available for the prevention of human brucellosis. in humans, brucellosis is mostly caused by brucella melitensis and brucella abortus. the outer membrane protein 31 (omp31) and l7/l12 are immunodominant and protective antigens conserved in human brucella pathogens. in the present study, we evaluated the humoral and cellular immune responses induced by a fusion protein designed based on the truncated form of om ... | 2015 | 25723468 |
| tcesr two-component regulatory system of brucella melitensis 16m is involved in invasion, intracellular survival and regulated cytotoxicity for macrophages. | the mechanisms of invasion and intracellular survival of brucella are still poorly understood. previous studies showed that the two-component regulatory systems (tcss) play an important role in the intracellular survival of brucella. to investigate if tcss involve in the virulence and cytotoxicity of brucella melitensis, we introduced a mutation into one of the tcss in chromosome ii in br. melitensis 16m strain, and generated 16mδtcesr, a mutant of br. melitensis 16m strain. in vitro infection e ... | 2015 | 25721466 |
| full-length cdna cloning, molecular characterization and differential expression analysis of peroxiredoxin 6 from ovis aries. | peroxiredoxin 6 (prdx6), an important antioxidant enzyme that can eliminate reactive oxygen species (ros) to maintain homeostasis, is a bifunctional protein that possesses the activities of both glutathione peroxidase and phospholipase a2. in this study, a novel full-length prdx6 cdna (oaprdx6) was cloned from sheep (ovis aries) using rapid amplification of cdna ends (race). the full-length cdna of oaprdx6 was 1753bp containing a 5'-untranslated region (utr) of 93bp, a 3'-utr of 985bp with a pol ... | 2015 | 25712755 |
| in vitro activity of brucella melitensis isolates to various antimicrobials in turkey. | brucellosis is a systemic infectious disease caused by brucella bacteria. a successful treatment requires antibiotics that can penetrate into the cell at high concentrations. the aim of this study was to assess the biotype and in vitro activity of 80 brucella isolates obtained from blood against various antimicrobials for human brucellosis in turkey. | 2015 | 25712728 |
| isolation and identification of brucella melitensis biovar 3 from vaccinated small ruminants: a public health threat in kosovo. | in 2011, a human brucellosis case with severe clinical symptoms was reported at the university clinic for infectious diseases in prishtina, kosovo. a trace-back investigation was conducted to find the source of human infection. a total of 49 blood samples and 15 corresponding milk samples from sheep and goats raised on the patient's farm were taken for serological and molecular analysis. serology using rbt and cft revealed 11 positive animals. twelve milk samples were pcr positive. a brucella st ... | 2016 | 25704160 |
| acute brucella melitensis m16 infection model in mice treated with tumor necrosis factor-alpha inhibitors. | there is limited data in the literature about brucellosis related to an intracellular pathogen and anti-tumor necrosis factor alpha (anti-tnfα) medication. the aim of this study was to evaluate acute brucella infections in mice receiving anti-tnfα drug treatment. | 2015 | 25699488 |
| using molecular tools to identify the geographical origin of a case of human brucellosis. | although malta is historically linked with the zoonosis brucellosis, there had not been a case of the disease in either the human or livestock population for several years. however, in july 2013 a case of human brucellosis was identified on the island. to determine whether this recent case originated in malta, four isolates from this case were subjected to molecular analysis. molecular profiles generated using multilocus sequence analysis and multilocus variable number tandem repeat for the rece ... | 2015 | 25697304 |
| blocking the expression of syntaxin 4 interferes with initial phagocytosis of brucella melitensis in macrophages. | brucella melitensis is the brucella species most frequently associated with brucellosis in humans. it is also the causative agent of the disease in goats and other ruminants. although significant aspects of the pathogenesis of infection by this intracellular pathogen have been clarified, several events during invasion of host cells remain to be elucidated. in this study, infections of human macrophages from the thp-1 monocyte cell line were conducted with b. melitensis bm133 wild-type strain and ... | 2015 | 25673907 |
| improved serodiagnosis of bovine brucellosis by novel synthetic oligosaccharide antigens representing the capping m epitope elements of brucella o-polysaccharide. | members of the genus brucella have cell wall characteristics of gram-negative bacteria, which in the most significant species includes o-polysaccharide (ops). serology is the most cost-effective means of detecting brucellosis, as infection with smooth strains of brucella leads to the induction of high antibody titers against the ops, an unbranched homopolymer of 4,6-dideoxy-4-formamido-d-mannopyranosyl residues (d-rha4nfo) that are variably α(1→2)- and α(1→3)-linked. six d-rha4nfo homo-oligosacc ... | 2015 | 25653412 |
| the effects of regm on stress responses in brucella melitensis. | brucella melitensis is a facultative intracellular pathogen. the regm gene encodes a sensory transduction protein kinase in b. melitensis 16m, and genes orthologous to regm have been found to exist in many bacterial species. however, little is known about the regulation function of this gene in brucella. in order to characterize this gene, we constructed a marked deletion mutant of regm as well as its complemented strain. the mutant was less able to withstand acid and hyperosmotic conditions tha ... | 2015 | 25648428 |
| identification of brucella spp. isolated from human brucellosis in malaysia using high-resolution melt (hrm) analysis. | molecular approaches have been investigated to overcome difficulties in identification and differentiation of brucella spp. using conventional phenotypic methods. in this study, high-resolution melt (hrm) analysis was used for rapid identification and differentiation of members of brucella genus. a total of 41 brucella spp. isolates from human brucellosis were subjected to hrm analysis using 4 sets of primers, which identified 40 isolates as brucella melitensis and 1 as brucella canis. the techn ... | 2015 | 25641125 |
| genotyping of human brucella melitensis biovar 3 isolated from shanxi province in china by mlva16 and hoof. | brucellosis presents a significant economic burden for china because it causes reproductive failure in host species and chronic health problems in humans. these problems can involve multiple organs. brucellosis is highly endemic in shanxi province china. molecular typing would be very useful to epidemiological surveillance. the purpose of this study was to assess the diversity of brucella melitensis strains for epidemiological surveillance. historical monitoring data suggest that brucella melite ... | 2015 | 25615697 |
| [study on the epidemiologic characteristic of brucella melitensis isolated in guizhou province in 2010-2012]. | to understand the genetic and epidemiologic characteristic of brucella (b.) melitensis strains isolated in guizhou province in 2010-2012. | 2014 | 25567021 |
| molecular cloning, expression and characterization of programmed cell death 10 from sheep (ovis aries). | programmed cell death 10 (pdcd10) is a highly conserved adaptor protein. its mutations result in cerebral cavernous malformations (ccms). in this study, pdcd10 cdna from the buffy coat of small tail han sheep (ovis aries) was cloned from a suppression subtractive hybridization cdna library, named oapdcd10. the full-length cdna of oapdcd10 was 1343bp with a 639bp open reading frame (orf) encoding 212 amino acid residues. tissue distribution of oapdcd10 mrna determined that it was ubiquitously exp ... | 2015 | 25541025 |
| melitococcosis in the republic of croatia. | melitococcosis is one of the most widespread zoonoses worldwide. in the period from 2009 to 2013, comprehensive melitococcosis testing was conducted in the republic of croatia. | 2014 | 25536995 |
| clinical manifestations, complications, and treatment of brucellosis: evaluation of 72 cases. | brucellosis is an important disease in developing countries. we aimed to determine the epidemiologic, clinical, and laboratory characteristics of brucellosis, which still has a high morbidity in turkey. | 2014 | 25536728 |
| hospital-associated transmission of brucella melitensis outside the laboratory. | brucella melitensis was identified in an aspirate obtained from a patient's hip joint during a procedure at a hospital in canada. we conducted an investigation into possible exposures among hospital workers; 1 worker who assisted with the procedure tested positive for b. melitensis. aerosol-generating procedures performed outside the laboratory may facilitate transmission of this bacterium. | 2015 | 25531198 |
| financial analysis of brucellosis control for small-scale goat farming in the bajío region, mexico. | brucellosis is an endemic disease in small-scale goat husbandry systems in mexico. it is a zoonosis and the economic consequences can be large, although estimates are not available for the mexican goat sector. our objective was to conduct a financial analysis of brucellosis control in a prominent dairy goat production area of the bajío region, mexico. we used three models: (1) a brucellosis transmission model at village flock level (n=1000 head), (2) a flock growth model at smallholder flock lev ... | 2015 | 25529385 |
| human brucellosis in maghreb: existence of a lineage related to socio-historical connections with europe. | despite control/eradication programs, brucellosis, major worldwide zoonosis due to the brucella genus, is endemic in northern africa and remains a major public health problem in the maghreb region (algeria/morocco/tunisia). brucella melitensis biovar 3 is mostly involved in human infections and infects mainly small ruminants. human and animal brucellosis occurrence in the maghreb seems still underestimated and its epidemiological situation remains hazy. this study summarizes official data, regar ... | 2014 | 25517901 |
| first case report of brucellosis in a child in thailand. | brucellosis is uncommon in children. in thailand, there have been only seven adult cases reported, all with brucella melitensis. we describe here the first reported pediatric case of brucellosis in thailand. a 12-year old boy presented with prolonged fever for one month, pancytopenia, pneumonia and peritonitis. the blood culture grew out brucella melitensis. he responded well to combination therapy consisting of doxycycline and gentamicin. he recovered fully without relapse during the 6 month fo ... | 2014 | 25507608 |
| bartonella henselae endocarditis in laos - 'the unsought will go undetected'. | both endocarditis and bartonella infections are neglected public health problems, especially in rural asia. bartonella endocarditis has been described from wealthier countries in asia, japan, korea, thailand and india but there are no reports from poorer countries, such as the lao pdr (laos), probably because people have neglected to look. | 2014 | 25503777 |
| in vitro bactericidal activity of aminoglycosides, including the next-generation drug plazomicin, against brucella spp. | plazomicin is a next-generation aminoglycoside with a potentially unique set of clinical characteristics compared with other aminoglycosides. this study assessed the minimum inhibitory concentrations (mics) and minimum bactericidal concentrations (mbcs) of plazomicin against 15 clinical isolates as well as three reference strains representing brucella abortus, brucella melitensis and brucella suis. these data were compared with those obtained for six other aminoglycosides and two aminocyclitols. ... | 2015 | 25459738 |
| proteomics-based identification of immunodominant proteins of brucellae using sera from infected hosts points towards enhanced pathogen survival during the infection. | brucella (b.) species lack classical virulence factors, but escape effectively the immune response of the host. the species brucella abortus and brucella melitensis infect predominantly cattle and small ruminants such as sheep or goats, respectively, but account also for most human cases. these two species share remarkably similar genomes but different proteomes have been demonstrated. this might be one of the reasons for their host specificity. a comprehensive identification of immunodominant p ... | 2015 | 25446124 |
| first case report of brucellosis in a child in thailand. | brucellosis is uncommon in children. in thailand, there have been only seven adult cases reported, all with brucella melitensis. we describe here the first reported pediatric case of brucellosis in thailand. a 12-year old boy presented with prolonged fever for one month, pancytopenia, pneumonia and peritonitis. the blood culture grew out brucella melitensis. he responded well to combination therapy consisting of doxycycline and gentamicin. he recovered fully without relapse during the 6 month fo ... | 2014 | 25427358 |
| let there be light! bioluminescent imaging to study bacterial pathogenesis in live animals and plants. | : bioluminescence imaging (bli) of bacteria was primarily designed to permit real-time, sensitive, and noninvasive monitoring of the progression of infection in live animals. generally, bli relies on the construction of bacterial strains that possess the lux operon. the lux operon is composed of a set of genes that encode the luciferase enzyme and its cognate substrate, which interact to produce light-a phenomenon that is referred to as bioluminescence. bioluminescence emitted by the bacteria ca ... | 2016 | 25395174 |
| septic arthritis caused by brucella melitensis in urban shenzhen, china: a case report. | brucellosis is a systemic infectious disease which is still a challenging medical problem in rural areas such as northern china. it rarely occurs in urban areas such as shenzhen in southern china. osteoarticular involvements are frequently seen in brucellosis, and rarely is arthritis the only clinical presentation. we report a case of hip septic arthritis caused by brucella melitensis in an urban area of shenzhen, china. | 2014 | 25394500 |
| isolated severe immune thrombocytopenia due to acute brucellosis. | mild anemia and leukopenia are the most common hematologic findings in the course of acute brucellosis. however severe form of thrombocytopenia is less frequently reported. the patient was admitted to the hospital with fever, gingival bleeding, and petechial skin lesions related to severe thrombocytopenia. he was investigated for the causes of thrombocytopenia. test results showed that wright agglutination test was positive at 1/5120 titer, and blood culture was positive for brucella melitensis. ... | 2014 | 25332526 |
| [clinical, serological and polymerase chain reaction follow-up of a family with brucellosis]. | human brucellosis diagnosis is based on isolation of brucella spp. from blood or tissue cultures with a positivity rate of 40-70% and serology techniques are used as complementary tools; recently molecular biology diagnostic techniques have been developed intending to optimize the etiological confirmation. | 2014 | 25327196 |
| thermostable cross-protective subunit vaccine against brucella species. | a subunit vaccine candidate was produced from brucella suis 145 (biovar 4; expressing both the a antigen of brucella abortus and the m antigen of brucella melitensis). the preparation consisted mostly of polysaccharide (ps; >90% [wt/wt]; both cell-associated ps and exo-ps were combined) and a small amount of protein (1 to 3%) with no apparent nucleic acids. vaccinated mice were protected (these had a statistically significant reduction in bacterial colonization compared to that of unvaccinated c ... | 2014 | 25320267 |
| detection of brucella melitensis in bovine milk and milk products from apparently healthy animals in egypt by real-time pcr. | brucellosis in egypt is an endemic disease among animals and humans. in endemic developing countries, dairy products produced from untreated milk are a potential threat to public health. the aim of this study was to detect brucellae in milk and milk products produced from apparently healthy animals to estimate the prevalence of contamination. | 2014 | 25313613 |
| abortion in cattle due to infection with staphylococcus lugdunensis. | an aborted fetus of 7 months gestation, the associated placenta, and a single blood sample from the dam were submitted for diagnostic investigation to the diagnostic laboratory of the lombardy and emilia-romagna experimental zooprophylactic institute in parma, italy. the serum was negative for neospora caninum, coxiella burnetii, chlamydophila abortus, bovine herpesvirus 1 (bhv-1), bovine viral diarrhea virus (bvdv), brucella abortus, and brucella melitensis. fetal tissues and placental cotyledo ... | 2014 | 25292193 |
| a new method for simultaneous gene deletion and down-regulation in brucella melitensis rev.1. | in this study, our aim was to integrate an antisense expression cassette in bacterial chromosome for providing a long-term expression down-regulation in a bid to develop a new approach for simultaneous deletion and down-regulation of target genes in bacterial system. therefore, we were used this approach for simultaneous deletion of the perosamine synthetase (per) gene and down-regulation of the virb1 expression in brucella melitensis rev.1. the per gene, which is one of the lps o-chain coding g ... | 2015 | 25249309 |
| molecular detection of brucella species in patients suspicious of brucellosis from zanjan, iran. | brucella is an intracellular pathogen capable of infecting animals and humans. the aim of this study was to identify brucella spp in sera of high risk individuals by a polymerase chain reaction (pcr)-based method. a total of 180 patients suspected to have brucellosis were examined by serological tests. to establish a pcr protocol for diagnosis of active brucellosis, dna was extracted from the serum samples by using a commercial kit. pcr amplification was done for detection of brocella dna using ... | 2014 | 25242938 |
| immunization of mice with a novel recombinant molecular chaperon confers protection against brucella melitensis infection. | brucella spp. are zoonotic gram-negative intracellular pathogens with the ability to survive and replicate in phagocytes. it has been shown that bacterial proteins expressed abundantly in this niche are stress-related proteins capable of triggering effective immune responses. bmei1549 is a molecular chaperone designated dnak that is expressed under stress conditions and helps to prevent formation of protein aggregates. in order to study the potential of dnak as a prospective brucella subunit vac ... | 2014 | 25240754 |
| renal abscess caused by brucella. | involvement of the renal parenchyma in the acute phase of brucellosis is very rare. only two cases of renal brucelloma have been reported in the english language literature to date. we report a case of renal abscess caused by brucella in the acute phase. a 45-year-old chinese man presented with a high fever, urine occult blood, and a low density lesion in the right kidney. ultrasound-guided aspiration was done. brucella melitensis was isolated from both blood and puncture fluid culture. minocycl ... | 2014 | 25220238 |
| replication of brucella abortus and brucella melitensis in fibroblasts does not require atg5-dependent macroautophagy. | several intracellular bacterial pathogens have evolved subtle strategies to subvert vesicular trafficking pathways of their host cells to avoid killing and to replicate inside the cells. brucellae are gram-negative facultative intracellular bacteria that are responsible for brucellosis, a worldwide extended chronic zoonosis. following invasion, brucella abortus is found in a vacuole that interacts first with various endosomal compartments and then with endoplasmic reticulum sub-compartments. bru ... | 2014 | 25179110 |
| sirna screens using drosophila cells to identify host factors required for infection. | drosophila melanogaster offers a powerful model system for interrogating interactions between host cells and human bacterial pathogens. brucella, a gram-negative, facultative intracellular bacterium is the causative agent of brucellosis, a zoonotic disease of global consequence. over the past several decades, pathogen factors that mediate brucella infection have been identified. however, host factors that mediate infection have remained obscure. we have used the power of the drosophila s2 cell s ... | 2014 | 25172284 |
| studying host-pathogen interaction events in living mice visualized in real time using biophotonic imaging. | despite progress in mouse models of bacterial pathogens, studies are often limited by evaluating infections in an individual organ or tissue or at a given time. here we present a technique to engineer the pathogen, e.g., brucella melitensis, with a bioluminescent marker permitting analysis of living bacteria in real time during the infectious process from acute to chronic infection. using this bioluminescent approach, tissue preference, differences between virulent and mutant bacteria, as well a ... | 2014 | 25172275 |
| galleria mellonella as an infection model for select agents. | the use of animal models is a key step to better understand bacterial virulence factors and their roles in host/pathogen interactions. to avoid the ethical and cost problems of mammalian models in bacterial virulence research, several insect models have been developed. one of these models, the larvae of the greater wax moth galleria mellonella, has been shown to be relevant for several fungal and bacterial mammalian pathogens. here, we describe the use g. mellonella to study virulence of the hig ... | 2014 | 25172272 |
| reemergence of brucella melitensis in wildlife, france. | 2014 | 25152274 | |
| shedding rates and seroprevalence of brucella melitensis in lactating goats of shahrekord, iran. | brucellosis remains a major worldwide zoonosis. caprine brucellosis is a significant problem for both public health and animal production. brucella melitensis causes disease in goats, sheep, humans, and occasionally cattle. transmission is by ingestion or contact with infected materials, vaginal discharge, or milk. | 2014 | 25147691 |
| liver histology of acute brucellosis caused by brucella melitensis. | as a major organ of the mononuclear phagocytic system, the liver is probably involved in all cases of brucellosis. in this prospective study, liver slides prepared from percutaneous liver biopsy samples of 20 patients with clinical and laboratory evidence of acute brucellosis due to brucella melitensis were examined for the presence or absence of granulomas by pathologists in iran and the united states. nineteen men and one woman ranging in age from 14 to 62 years were studied. all patients had ... | 2014 | 25147098 |
| decreased in vivo virulence and altered gene expression by a brucella melitensis light-sensing histidine kinase mutant. | brucella species utilize diverse virulence factors. previously, brucella abortus light-sensing histidine kinase was identified as important for cellular infection. here, we demonstrate that a brucella melitensis lov-hk (bm-lov-hk) mutant strain has strikingly different gene expression than wild type. general stress response genes including the alternative sigma factor rpoe1 and its anti-anti-sigma factor phyr were downregulated, while flagellar, quorum sensing (qs), and type iv secretion system ... | 2015 | 25132657 |
| identification and in vitro antimicrobial susceptibility of brucella species isolated from human brucellosis. | brucellosis is a world-wide zoonotic disease with a major impact on the public health. due to the high risk of laboratory acquired infection, limited laboratory investigations were performed on this organism, including detailed identification and susceptibility study. brucella melitensis is the commonest aetiological agent for human brucellosis in this region. the in vitro susceptibility pattern against selected antimicrobial agents was assessed using e-test. all isolates were noted to be sensit ... | 2014 | 25120569 |
| inactivation of bacterial and viral biothreat agents on metallic copper surfaces. | in recent years several studies in laboratory settings and in hospital environments have demonstrated that surfaces of massive metallic copper have intrinsic antibacterial and antiviral properties. microbes are rapidly inactivated by a quick, sharp shock known as contact killing. the underlying mechanism is not yet fully understood; however, in this process the cytoplasmic membrane is severely damaged. pathogenic bacterial and viral high-consequence species able to evade the host immune system a ... | 2014 | 25100640 |
| [distribution of potential pathogenic bacteria in the jiulong river watershed]. | recently, the human activities including economic growth and urbanization posed serious environmental health risks to the jiulong river watershed (jrw). in order to gain a full understanding of the distribution of potential pathogenic bacteria (ppb) in this area, we used 16s rrna amplicon pyrosequencing technology to investigate planktonic and benthic bacterial community in two main tributaries (north river, nr, and west river, wr) of the jiulong river (jr). at the genus level, a total of 68 gen ... | 2014 | 25055661 |
| evaluation of a brucella melitensis mutant deficient in o-polysaccharide export system atp-binding protein as a rough vaccine candidate. | rough brucella mutants have been sought as vaccine candidates that do not interfere with the conventional serological diagnosis of brucellosis. in this study, a rough mutant of brucella melitensis was generated by the disruption of the wzt gene, which encodes the o-polysaccharide (o-ps) export system atp-binding protein. in vivo, the mutant 16mδwzt was attenuated and conferred a level of protection against b. melitensis 16m challenge similar to that conferred by the vaccine strain b. melitensis ... | 2014 | 25043564 |
| recovery of a medieval brucella melitensis genome using shotgun metagenomics. | shotgun metagenomics provides a powerful assumption-free approach to the recovery of pathogen genomes from contemporary and historical material. we sequenced the metagenome of a calcified nodule from the skeleton of a 14th-century middle-aged male excavated from the medieval sardinian settlement of geridu. we obtained 6.5-fold coverage of a brucella melitensis genome. sequence reads from this genome showed signatures typical of ancient or aged dna. despite the relatively low coverage, we were ab ... | 2014 | 25028426 |
| brucella melitensis invades murine erythrocytes during infection. | brucella spp. are facultative intracellular gram-negative coccobacilli responsible for brucellosis, a worldwide zoonosis. we observed that brucella melitensis is able to persist for several weeks in the blood of intraperitoneally infected mice and that transferred blood at any time point tested is able to induce infection in naive recipient mice. bacterial persistence in the blood is dramatically impaired by specific antibodies induced following brucella vaccination. in contrast to bartonella, t ... | 2014 | 25001604 |
| identification of a new immunogenic candidate conferring protection against brucella melitensis infection in mice. | identification of bacterial proteins that contribute to the replication and survival of the engulfed bacteria within phagolysosome is critical in the pathogenesis of intracellular bacteria. heat shock proteins (hsps) are molecular chaperones that prevent unwanted protein aggregation and protect the bacteria against cell stress. in order to study the potential of hspa for development of a brucella subunit vaccine, immunogenicity and protective efficacy of recombinant hspa (rhspa) from brucella me ... | 2014 | 24995396 |
| a functional and phylogenetic comparison of quorum sensing related genes in brucella melitensis 16m. | a quorum-sensing (qs) system is involved in brucella melitensis survival inside the host cell. two transcriptional regulators identified in b. melitensis, blxr and vjbr, regulate the expression of virb, an operon required for bacterial intracellular persistence. in this work, 628 genes affected by vjbr and 124 by blxr were analyzed to gain insights into their functional and taxonomical distributions among the bacteria and archaea cellular domains. in this regard, the cluster of orthologous group ... | 2014 | 24994008 |
| advancement of knowledge of brucella over the past 50 years. | fifty years ago, bacteria in the genus brucella were known to cause infertility and reproductive losses. at that time, the genus was considered to contain only 3 species: brucella abortus, brucella melitensis, and brucella suis. since the early 1960s, at least 7 new species have been identified as belonging to the brucella genus (brucella canis, brucella ceti, brucella inopinata, brucella microti, brucella neotomae, brucella ovis, and brucella pinnipedialis) with several additional new species u ... | 2014 | 24981716 |
| main functions and taxonomic distribution of virulence genes in brucella melitensis 16 m. | many virulence genes have been detected in attenuated mutants of brucella melitensis 16 m; nevertheless, a complete report of these genes, including the main cluster of orthologous groups (cog) represented as well as the taxonomical distribution among all complete bacterial and archaeal genomes, has not been analyzed. in this work a total of 160 virulence genes that have been reported in attenuated mutants in b. melitensis were included and analyzed. additionally, we obtained 250 b. melitensis r ... | 2014 | 24964015 |
| a repa-based elisa for discriminating cattle vaccinated with brucella suis 2 from those naturally infected with brucella abortus and brucella melitensis. | the commonest ways of diagnosing brucellosis in animals include the rose-bengal plate agglutination test, the buffered plate agglutination test (bpa), the slide agglutination test, the complement fixation test, and the indirect enzyme linked immunosorbent assay (i-elisa). however, these methods cannot discriminate the brucella vaccine strain (brucella suis strain 2; b. suis s2) from naturally acquired virulent strains. of the six common brucella species, brucella melitensis, brucella abortus, an ... | 2014 | 24941369 |
| emergence of quinolone-resistant, topoisomerase-mutant brucella after treatment with fluoroquinolones in a macrophage experimental infection model. | to determine the activity of fluoroquinolones (fq) and the selection of fq-resistant mutants in a macrophage experimental infection model (meim). | 2015 | 24913992 |
| brucella melitensis sternal osteomyelitis following median sternotomy. | human brucellosis, a zoonotic infection, may present with a range of symptoms but is rarely described as a cause of surgical site infections. we present the first reported case of brucella melitensis causing sternal osteomyelitis of a midline sternotomy for a coronary artery bypass graft. the operation was performed in a non-endemic country but the patient had travelled to syria immediately before surgery, where the infection was assumed to have been acquired. the infection resolved following tr ... | 2014 | 24910374 |
| fad binding, cobinamide binding and active site communication in the corrin reductase (cobr). | adenosylcobalamin, the coenzyme form of vitamin b12, is one nature's most complex coenzyme whose de novo biogenesis proceeds along either an anaerobic or aerobic metabolic pathway. the aerobic synthesis involves reduction of the centrally chelated cobalt metal ion of the corrin ring from co(ii) to co(i) before adenosylation can take place. a corrin reductase (cobr) enzyme has been identified as the likely agent to catalyse this reduction of the metal ion. herein, we reveal how brucella melitensi ... | 2014 | 24909839 |
| a protein-conjugate approach to develop a monoclonal antibody-based antigen detection test for the diagnosis of human brucellosis. | human brucellosis is most commonly diagnosed by serology based on agglutination of fixed brucella abortus as antigen. nucleic acid amplification techniques have not proven capable of reproducibly and sensitively demonstrating the presence of brucella dna in clinical specimens. we sought to optimize a monoclonal antibody-based assay to detect brucella melitensis lipopolysaccharide in blood by conjugating b. melitensis lps to keyhole limpet hemocyanin, an immunogenic protein carrier to maximize ig ... | 2014 | 24901521 |
| evaluation in mice of brucella ovis attenuated mutants for use as live vaccines against b. ovis infection. | brucella ovis causes ram contagious epididymitis, a disease for which a specific vaccine is lacking. attenuated brucella melitensis rev 1, used as vaccine against ovine and caprine brucellosis caused by b. melitensis, is also considered the best vaccine available for the prophylaxis of b. ovis infection, but its use for this purpose has serious drawbacks. in this work, two previously characterized b. ovis attenuated mutants (δomp25d and δomp22) were evaluated in mice, in comparison with b. melit ... | 2014 | 24898325 |
| draft genome sequence of the field isolate brucella melitensis strain bm ind1 from india. | brucella spp. are facultative intracellular bacterial pathogens causing the zoonotic disease brucellosis. here, we report the draft genome sequence of the brucella melitensis strain from india designated bm ind1, isolated from stomach contents of an aborted goat fetus. | 2014 | 24874680 |
| the cyclic amp-binding protein cbpb in brucella melitensis and its role in cell envelope integrity, resistance to detergent and virulence. | brucella melitensis possesses an operon with two components: the response regulator otpr and a putative camp-dependent protein kinase regulatory subunit encoded by the bmei0067 gene. in the previous study, the function of otpr has been studied, while little is known about the function of the bmei0067 gene. using a bioinformatics approach, we showed that the bmei0067 gene encodes an additional putative camp-binding protein, which we refer to as cbpb. structural modeling predicted that cbpb has a ... | 2014 | 24850100 |
| in silico analysis of chimeric tf, omp31 and bp26 fragments of brucella melitensis for development of a multi subunit vaccine candidate. | brucellosis, especially caused by brucella melitensis, remains one of the most common zoonotic diseases worldwide with more than 500,000 human cases reported annually. the commonly used live attenuated vaccine in ovine brucellosis prophylaxis is b. melitensis rev1. but due to different problems caused by the administration of this vaccine, a protective subunit vaccine against b. melitensis is strongly demanded. brucella bp26, omp31 and tf proteins have shown a considerable potential as protectiv ... | 2014 | 24847419 |
| serological survey of brucella canis in dogs in urban harare and selected rural communities in zimbabwe. | a cross-sectional study was conducted in order to detect antibodies for brucella canis (b. canis) in dogs from urban harare and five selected rural communities in zimbabwe. sera from randomly selected dogs were tested for antibodies to b. canis using an enzyme-linked immunosorbent assay. overall, 17.6% of sera samples tested (57/324, 95% ci: 13.5-21.7) were positive for b. canis antibodies. for rural dogs, seroprevalence varied from 11.7% - 37.9%. rural dogs recorded a higher seroprevalence (20. ... | 2014 | 24830899 |
| interferon-gamma responses in sheep exposed to virulent and attenuated brucella melitensis strains. | antibody detection is the basis of large-scale sheep brucellosis diagnosis because of its sensitivity and specificity. in contrast, information on the cellular mediated immune (cmi) response triggered after brucella melitensis infection, a cornerstone in the protection against this pathogen, is more limited, particularly regarding the effect of the virulence of the infecting strain in the induced cmi reaction. here, the interferon-gamma (ifn-γ) profiles evoked after exposure by different routes ... | 2014 | 24814244 |
| modelling risk aversion to support decision-making for controlling zoonotic livestock diseases. | zoonotic infectious livestock diseases are becoming a significant burden for both animal and human health and are rapidly gaining the attention of decision-makers who manage public health programmes. if control decisions have only monetary components, governments are generally regarded as being risk-neutral and the intervention strategy with the highest expected benefit (lowest expected net costs) should be preferred. however, preferences will differ and alternative intervention plans will preva ... | 2013 | 24761718 |
| three-dimensional (3d) structure prediction of the american and african oil-palms β-ketoacyl-[acp] synthase-ii protein by comparative modelling. | the fatty-acid profile of the vegetable oils determines its properties and nutritional value. palm-oil obtained from the african oil-palm [elaeis guineensis jacq. (tenera)] contains 44% palmitic acid (c16:0), but, palm-oil obtained from the american oilpalm [elaeis oleifera] contains only 25% c16:0. in part, the b-ketoacyl-[acp] synthase ii (kasii) [ec: 2.3.1.179] protein is responsible for the high level of c16:0 in palm-oil derived from the african oil-palm. to understand more about e. guineen ... | 2014 | 24748752 |
| brucella melitensis in france: persistence in wildlife and probable spillover from alpine ibex to domestic animals. | bovine brucellosis is a major zoonosis, mainly caused by brucella abortus, more rarely by brucella melitensis. france has been bovine brucellosis officially-free since 2005 with no cases reported in domestic/wild ruminants since 2003. in 2012, bovine and autochthonous human cases due to b. melitensis biovar 3 (bmel3) occurred in the french alps. epidemiological investigations implemented in wild and domestic ruminants evidenced a high seroprevalence (>45%) in alpine ibex (capra ibex); no cases w ... | 2014 | 24732322 |
| the inva gene of brucella melitensis is involved in intracellular invasion and is required to establish infection in a mouse model. | some of the mechanisms underlying the invasion and intracellular survival of b. melitensis are still unknown, including the role of a subfamily of nudix enzymes, which have been described in other bacterial species as invasins and are present in brucella spp. we have generated a mutation in the coding gene of one of these proteins, the inva gene (bmei0215) of b. melitensis strain 133, to understand its role in virulence. hela cell invasion results showed that mutant strain survival was decreased ... | 2014 | 24667775 |
| cold shock protein a plays an important role in the stress adaptation and virulence of brucella melitensis. | brucella melitensis is a facultative intracellular pathogen that mainly resides within macrophages. the mechanisms employed by brucella to adapt to harsh intracellular environments and survive within host macrophages are not clearly understood. here, we constructed a cspa gene deletion mutant, niδcspa, that did not exhibit any discernible growth defect at a normal culture temperature (37 °c) or at a low temperature (15 °c). however, expression of the cspa gene in brucella was induced by cold, ac ... | 2014 | 24661136 |
| crystal structure and biochemical studies of brucella melitensis 5'-methylthioadenosine/s-adenosylhomocysteine nucleosidase. | the prokaryotic 5'-methylthioadenosine/s-adenosylhomocysteine nucleosidase (mtan) catalyzes the irreversible cleavage of the glycosidic bond in 5'-methylthioadenosine (mta) and s-adenosylhomocysteine (sah), a process that plays a key role in several metabolic pathways. its absence in all mammalian species has implicated this enzyme as a promising target for antimicrobial drug design. here, we report the crystal structure of bmmtan in complex with its product adenine at a resolution of 2.6 å dete ... | 2014 | 24657441 |
| humoral immunity and cd4+ th1 cells are both necessary for a fully protective immune response upon secondary infection with brucella melitensis. | brucella spp are intracellular bacteria that cause brucellosis, one of the most common zoonoses in the world. given the serious medical consequences of this disease, a safe and effective human vaccine is urgently needed. efforts to develop this vaccine have been hampered by our lack of understanding of what constitutes a protective memory response against brucella. in this study, we characterize the cells and signaling pathways implicated in the generation of a protective immune memory response ... | 2014 | 24646742 |
| proteomic analysis of detergent resistant membrane domains during early interaction of macrophages with rough and smooth brucella melitensis. | the plasma membrane contains discrete nanometer-sized domains that are resistant to non-ionic detergents, and which are called detergent resistant membrane domains (drmds) or lipid rafts. exposure of host cells to pathogenic bacteria has been shown to induce the re-distribution of specific host proteins between drmds and detergent soluble membranes, which leads to the initiation of cell signaling that enable pathogens to access host cells. drmds have been shown to play a role in the invasion of ... | 2014 | 24643124 |
| case-control study on risk factors associated with brucella melitensis in goat farms in peninsular malaysia. | caprine brucellosis is a bacterial zoonotic infection affecting goats especially in developing countries all over the world. in malaysia, the risk factors associated with this infection in farms have not been studied. a case-control study was carried out in goat farms in four states of malaysia to elucidate the risk factors associated with the infection on the farms using structured questionnaires and face-to-face interviews. results indicate that the introduction of new animals (or = 5.25; 90 % ... | 2014 | 24633657 |
| [two spondylodiscitis due to a contamination by brucella melitensis in a mother and daughter back from holidays]. | we describe here a case of spondylodiscitis of brucellosis origin in one patient back from turkey. some weeks later, her mother, who had accompagnied her in turkey also developped similar symptoms. a diagnosis of spondylodiscitis due to a contamination by brucella melitensis was also proposed. since the control of animal brucellosis in europe, human cases are rarer. sporadic cases still observed are mostly travellers back from aerea where animal brucellosis remains endemic. seroprevalence of a s ... | 2014 | 24624738 |
| seroprevalence for coxiella burnetii, francisella tularensis, brucella abortus and brucella melitensis in austrian adults: a cross-sectional survey among military personnel and civilians. | the prevalence of coxiella burnetii, francisella tularensis, brucella abortus, and brucella melitensis infections in austria and the exposure risk of military personnel were assessed in an exploratory nationwide cross-sectional seroprevalence survey in 526 healthy adult individuals, 222 of which were soldiers and 304 were civilians. screening for iga/igg antibodies to c. burnetii (phase i) and igg/igm antibodies to c. burnetii (phase ii), and to f. tularensis was done with commercial enzyme-link ... | 2014 | 24572607 |
| collapsed l4 vertebral body caused by brucellosis. | brucellosis is caused by gram-negative, aerobic, non-motile, facultative, intracellular coccobacilli belonging to the genus brucella. a 50-year-old man working as an employee was admitted to neurosurgery clinic with severe low back, radicular right leg pain and hypoesthesia in right l4-5 dermatomes for 2 months. brucella tube agglutination (wright) test was positive in serum sample of the patient with a titer of 1/640. brucella melitensis was isolated from blood culture. x-ray and mri of the lom ... | 2014 | 24570819 |
| preliminary report on the therapeutic action of methymycin in brucella melitensis infection. | 1954 | 24542881 | |
| comparative whole genome analysis of six diagnostic brucellaphages. | whole genome sequencing of six diagnostic brucellaphages, tbilisi (tb), firenze (fz), weybridge (wb), s708, berkeley (bk) and r/c, was followed with genomic comparisons including recently described genomes of the tb phage from mexico (tbm) and pr phage to elucidate genomic diversity and candidate host range determinants. comparative whole genome analysis revealed high sequence homogeneity among these brucellaphage genomes and resolved three genetic groups consistent with defined host range pheno ... | 2014 | 24530704 |
| validation of a simple universal ielisa for the diagnosis of human brucellosis. | the definitive diagnosis of brucellosis requires isolation of the agent, although negative isolation does not rule out the infection. in contrast, serological testing is more sensitive and, therefore, preferred in clinical practice. the majority of reported cases around the world were caused by brucella melitensis, b. abortus, b. suis and b. canis. the first three species contain o-polysaccharide (ops) on the cell surface, but b. canis contains no measurable ops on the rough lipopolysaccharide ( ... | 2014 | 24515098 |
| assessment of genetic diversity of zoonotic brucella spp. recovered from livestock in egypt using multiple locus vntr analysis. | brucellosis is endemic in most parts of egypt, where it is caused mainly by brucella melitensis biovar 3, and affects cattle and small ruminants in spite of ongoing efforts devoted to its control. knowledge of the predominant brucella species/strains circulating in a region is a prerequisite of a brucellosis control strategy. for this reason a study aiming at the evaluation of the phenotypic and genetic heterogeneity of a panel of 17 brucella spp. isolates recovered from domestic ruminants (catt ... | 2014 | 24511531 |
| evaluation of the immunogenicity and safety of brucella melitensis b115 vaccination in pregnant sheep. | in spite of its limitations, rev.1 is currently recognized as the most suitable vaccine against brucella melitensis (the causative agent of ovine and caprine brucellosis). however, its use is limited to young animals when test-and-slaughter programs are in place because of the occurrence of false positive-reactions due to rev.1 vaccination. the b. melitensis b115 rough strain has demonstrated its efficacy against b. melitensis virulent strains in the mouse model, but there is a lack of informati ... | 2014 | 24508034 |
| brucella endocarditis in prosthetic valves. | none declared. | 2012 | 24493988 |
| genetic polymorphism characteristics of brucella canis isolated in china. | in china, brucellosis is an endemic disease typically caused by brucella melitensis infection (biovars 1 and 3). brucella canis infection in dogs has not traditionally recognized as a major problem. in recent years however, brucellosis resulting from brucella canis infection has also been reported, suggesting that infections from this species may be increasing. data concerning the epidemiology of brucellosis resulting from brucella canis infection is limited. therefore, the purpose of this study ... | 2014 | 24465442 |
| a pregnant mouse model for the vertical transmission of brucella melitensis. | abortion is the major clinical sign of brucellosis in animals but little is known about the underlying mechanisms. this study was designed to evaluate a pregnant mouse model for the vertical transmission of brucella melitensis using four infectious doses: 10(3) colony-forming units (cfu), 10(4) cfu, 10(5) cfu, and 10(6) cfu. during the experimental period, no instances of abortion were recorded, but stillbirths were observed in the groups infected with doses of 10(4) cfu and higher. regardless o ... | 2014 | 24462801 |
| brucella endocarditis of pseudoaneurysm of an aortic composite graft. | the occurrence of brucella endocarditis following bentall operation is a rare and life threatening condition, particularly when it is complicated by development of a pseudoaneurysm. here we present a 40-year-old veterinarian with bicuspid aortic valve, who developed type a aortic root dissection and required bentall operation. his past medical history was positive for an episode of treated brucellosis. four months after the operation, he developed signs, symptoms and serological findings of bruc ... | 2013 | 24459566 |
| evaluation of different enzyme-linked immunosorbent assays for the diagnosis of brucellosis due to brucella melitensis in sheep. | six serological assays for the diagnosis of ovine brucellosis, due to brucella melitensis were evaluated. reference serum samples from sheep of known b. melitensis infection status (n=118) were assessed using the rose bengal test (rbt), complement fixation test (cft) and four commercial enzyme-linked immunosorbent assays (elisas), including two indirect elisas (ielisas), one competitive elisa (celisa) and one blocking elisa (belisa). the highest differential positive rates (dpr) were obtained wi ... | 2014 | 24456797 |
| 16s rrna and omp31 gene based molecular characterization of field strains of b. melitensis from aborted foetus of goats in india. | brucellosis is a reemerging infectious zoonotic disease of worldwide importance. in human, it is mainly caused by brucella melitensis, a natural pathogen for goats. in india, a large number of goats are reared in semi-intensive to intensive system within the close vicinity of human being. at present, there is no vaccination and control strategy for caprine brucellosis in the country. thus, to formulate an effective control strategy, the status of etiological agent is essential. to cope up with t ... | 2013 | 24453799 |
| brucella melitensis shunt infection. | 2014 | 24441347 | |
| antimicrobial susceptibility data for brucella melitensis isolates cultured from uk patients. | 2014 | 24440739 | |
| splenic abscess due to brucellosis: a case report and a review of the literature. | splenic abscess due to acute brucellosis is a rare event. we report a case of multiple splenic abscesses caused by brucella melitensis in a 45-year-old woman and review the english language literature based on a pubmed/medline search of the last 50 years. the majority of the cases published in the literature were due to b. melitensis and a splenectomy was required in half of the cases. antibiotics alone without surgical intervention can be successful in the treatment of patients with splenic bru ... | 2014 | 24433982 |
| reverse line blot macroarray for simultaneous detection and characterization of four biological warfare agents. | the need for a rapid detection and characterization of biowarfare (bw) agents cannot be over emphasized. with diverse array of potential bw pathogen available presently, rapid identification of the pathogen is crucial, so that specific therapy and control measures can be initiated. we have developed a multiplex polymerase chain reaction based reverse line blot macroarray to simultaneously detect four pathogens of bw importance viz. bacillus anthracis, yersinia pestis, brucella melitensis and bur ... | 2013 | 24426077 |
| immunotherapeutics to prevent the replication of brucella in a treatment failure mouse model. | outer membrane vesicles (omvs) from brucella melitensis and irradiated brucella neotomae have been shown to be effective vaccines against a b. melitensis challenge in a mouse model. the present study evaluates the efficacy of these two vaccines as immuno-therapeutics in combination with conventional antibiotics against a b. melitensis infection. balb/c mice chronically infected with b. melitensis were treated for 4 weeks with doxycycline and gentamicin and vaccinated twice during the course of t ... | 2014 | 24397905 |
| increasing threat of brucellosis to low-risk persons in urban settings, china. | cases of brucellosis were diagnosed in 3-month-old twins and their mother. an epidemiologic survey suggested that raw sheep or goat meat might be the source of brucella melitensis infection. this finding implies that the increasing threat of brucellosis might affect low-risk persons in urban settings in china. | 2014 | 24377827 |
| evaluation of humoral and cellular immune responses to bp26 and omp31 epitopes in the attenuated brucella melitensis vaccinated sheep. | in recent years, the number of cases of human brucellosis has been increasing by approximately 10% per year in china. most cases were caused by brucella melitensis through contacts with infected sheep, goats or their products. an attenuated b. melitensis vaccine m5-90 is currently used to vaccinate both animals in china. this vaccine has not been investigated for critical parameters such as immune response and its association with protective efficacy. in this study, humoral and cellular immune r ... | 2014 | 24370708 |
| aerosol-induced brucellosis increases tlr-2 expression and increased complexity in the microanatomy of astroglia in rhesus macaques. | brucella melitensis, a bacterial pathogen and agent of epizootic abortion causes multiple pathologies in humans as well as a number of agriculturally important animal species. clinical human brucellosis manifests as a non-specific, chronic debilitating disease characterized by undulant fever, arthropathies, cardiomyopathies and neurological sequelae. these symptoms can occur acutely for a few weeks or persist for months to years. within the brain, endothelial and glial cells can be infected lead ... | 2013 | 24350061 |
| quorum sensing and self-quorum quenching in the intracellular pathogen brucellamelitensis. | brucella quorum sensing has been described as an important regulatory system controlling crucial virulence determinants such as the virb type iv secretion system and the flagellar genes. however, the basis of quorum sensing, namely the production of autoinducers in brucella has been questioned. here, we report data obtained from the use of a genetic tool allowing the in situ detection of long-chain n-acyl-homoserine lactones (ahl) activity at single bacterium level in brucella melitensis. these ... | 2013 | 24349302 |
| systems biology analysis of brucella infected peyer's patch reveals rapid invasion with modest transient perturbations of the host transcriptome. | brucella melitensis causes the most severe and acute symptoms of all brucella species in human beings and infects hosts primarily through the oral route. the epithelium covering domed villi of jejunal-ileal peyer's patches is an important site of entry for several pathogens, including brucella. here, we use the calf ligated ileal loop model to study temporal in vivo brucella-infected host molecular and morphological responses. our results document brucella bacteremia occurring within 30 min afte ... | 2013 | 24349118 |
| brucella induces an unfolded protein response via tcpb that supports intracellular replication in macrophages. | brucella melitensis is a facultative intracellular bacterium that causes brucellosis, the most prevalent zoonosis worldwide. the brucella intracellular replicative niche in macrophages and dendritic cells thwarts immune surveillance and complicates both therapy and vaccine development. currently, host-pathogen interactions supporting brucella replication are poorly understood. brucella fuses with the endoplasmic reticulum (er) to replicate, resulting in dramatic restructuring of the er. this er ... | 2013 | 24339776 |