Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| evaluation of the transcription level of the protein disulfide isomerase in different stages from ancylostoma caninum with a real-time pcr assay. | the protein disulfide isomerase (pdi) is a ubiquitous protein, which contributes in building disulfide bridges. in the work presented here, the expression of the pdi in different stages of the canine hookworm ancylostoma caninum was investigated. third-stage larvae (l3), adults, as well as serum-stimulated and hypobiotic l3 were used. for quantification of the pdi gene transcription, a real-time pcr was used establishing a hybridization probe (taqmantrade mark probes) for detection of pdi copy n ... | 2007 | 17762941 |
| pyrantel in small animal medicine: 30 years on. | pyrantel, a tetrahydropyrimidine nicotinic agonist anthelmintic, has been used in companion animal medicine since the 1970s to control two important nematode groups, the hookworms and the roundworms. given the zoonotic potential of these parasites, pyrantel has served a dual role in helping to protect the health of both companion animals and the public for more than 30 years. this review describes the history and mechanism of action of this drug, and collates evidence that resistance to pyrantel ... | 2008 | 17720556 |
| the evaluation of recombinant hookworm antigens as vaccines in hamsters (mesocricetus auratus) challenged with human hookworm, necator americanus. | we have previously reported the successful adaptation of human hookworm necator americanus in the golden hamster, mesocricetus auratus. this animal model was used to test a battery of hookworm (n. americanus and ancylostoma caninum) recombinant antigens as potential vaccine antigens. hamsters immunized a leading vaccine candidate n. americanus-ancylostoma secreted protein 2 (na-asp-2) and challenged with n. americanus infective larvae (l3), resulted in 30-46.2% worm reduction over the course of ... | 2008 | 17645877 |
| gastrointestinal nematodes in dogs from debre zeit, ethiopia. | the study was conducted during the period between january 2005 and june 2006 to determine the frequency of gastrointestinal (gi) nematode infections of dogs in and around debre zeit, using qualitative and quantitative coprological (n = 100) and postmortem examinations (n = 20). by coproscopy 51% dogs were positive for different types of nematodal eggs, out of which 23.5% were with mixed infections. on necropsy 95% animals were found positive for adult parasites, of which 31.6% were showing more ... | 2007 | 17614203 |
| recombinant nematode anticoagulant protein c2 in patients with non-st-segment elevation acute coronary syndrome: the anthem-timi-32 trial. | we sought to evaluate the safety and efficacy of recombinant nematode anticoagulant protein c2 (rnapc2) in patients with non-st-segment elevation acute coronary syndrome (nste-acs). | 2007 | 17599602 |
| active and exo-site inhibition of human factor xa: structure of des-gla factor xa inhibited by nap5, a potent nematode anticoagulant protein from ancylostoma caninum. | hookworms are hematophagous nematodes capable of growth, development and subsistence in living host systems such as humans and other mammals. approximately one billion, or one in six, people worldwide are infected by hookworms causing gastrointestinal blood loss and iron deficiency anemia. the hematophagous hookworm ancylostoma caninum produces a family of small, disulfide-linked protein anticoagulants (75-84 amino acid residues). one of these nematode anticoagulant proteins, nap5, inhibits the ... | 2007 | 17588602 |
| echinococcosis (zoonotic hydatidosis) in street dogs in urban and rural areas, dakahlia governorate, egypt. | a total of one hundred and ninety street dogs were captured from urban area, mansoura district and three hundreds and fifty from rural area, met el-korama and adjacent villages (manshet el-badawy, talka center). the overall prevalence of echinoccocus granulosus was 5%, with a worm burden ranging from 4 to 1010 (mean = 421). the prevalence was 6% in rural locality and 3.2% in urban locality. e. granulosus in dogs was significantly higher in rural areas but, without significant difference in puppi ... | 2007 | 17580584 |
| arthrostoma miyazakiense (nematoda: ancylostomatidae) infection in raccoon dogs of korea and experimental transmission to dogs. | arthrostoma miyazakiense (nematoda: ancylostomatidae) is a hookworm species reported from the small intestines of raccoon dogs (nyctereutes procyonoides) in japan. five korean raccoon dogs (n. procyonoides koreensis) caught from 2002 to 2005 in jeollanam-do (province), a southeastern area of south korea, contained helminth eggs belonging to 4 genera (roundworm, hookworm, whipworm, and capillaria spp.) and cysts of giardia sp. in their feces. necropsy findings of 1 raccoon dog revealed a large nu ... | 2007 | 17570975 |
| protective immunity elicited by ultraviolet-irradiated third-stage infective hookworm (necator americanus and ancylostoma caninum) larvae in mice and hamsters. | the protective immunity elicited by ultraviolet-irradiated third-stage infective larvae of necator americanus (uv-nal3) and ancylostoma caninum (uv-acl3) was evaluated in laboratory mice (a non-permissive model) and hamsters (a permissive model). after optimizing the time of exposure to uv-irradiation, both oral and subcutaneous vaccination routes with uv-acl3 in mice were explored. oral vaccination was more effective at reducing the number of challenge acl3 entering the lungs, whereas subcutane ... | 2006 | 17333729 |
| new anticoagulants for the prevention and treatment of venous thromboembolism. | anticoagulant therapy is effective at preventing the development of venous thromboembolism in high-risk patients, and reduces morbidity and mortality in individuals with established thromboembolic disease. vitamin k antagonists and heparins are currently the most commonly used anticoagulant drugs, but they have practical limitations. therefore, new antithrombotic agents with predictable dose-responses (thereby decreasing the need for monitoring without compromising efficacy or safety), ideally a ... | 2005 | 17319097 |
| molecular cloning and characterization of ac-mtp-2, an astacin-like metalloprotease released by adult ancylostoma caninum. | ac-mtp-2 is an astacin-like metalloprotease secreted by adult ancylostoma caninum hookworms. ac-mtp-2 cdna was cloned by immunoscreening a cdna library with antisera prepared against adult a. caninum excretory/secretory (es) products. the full-length ac-mtp-2 contains 850 bp cdna encoding a 233 amino acid open reading frame (orf) with 32% amino acid identity to ce-nsp-4, a pharyngeal cell-derived secreted metalloprotease of the nematode caenorhabditis elegans. the predicted orf contained a conse ... | 2007 | 17280728 |
| reduction of worm fecundity and canine host blood loss mediates protection against hookworm infection elicited by vaccination with recombinant ac-16. | hookworm infection is one of most important parasitic infection of humans, occurring in 740 million people. here we report the protective vaccination of dogs with ac-16, an immunodominant surface antigen from the hookworm ancylostoma caninum. we show that immunization with ac-16 formulated with as03 elicited specific humoral and cellular immune responses and provided partial protection against hookworm infection and morbidity as evidenced by a significant reduction of hookworm egg counts (64% re ... | 2007 | 17267592 |
| field evaluation of the efficacy and the safety of a combination of oxantel/pyrantel/praziquantel in the treatment of naturally acquired gastrointestinal nematode and/or cestode infestations in dogs in europe. | in five multicentre field trials, the efficacy and safety of a combination of oxantel/pyrantel/praziquantel (dolpac), vetoquinol sa) in the treatment of naturally acquired gastrointestinal nematode and/or cestode infestation in dogs was evaluated in northern and southern europe. forty-eight investigators from france, belgium, germany, italy and spain enrolled 329 dogs to be treated with the tested combination; 235 of these dogs complied with the inclusion criteria of the protocol and had a teste ... | 2007 | 17184919 |
| intermolecular interactions and characterization of the novel factor xa exosite involved in macromolecular recognition and inhibition: crystal structure of human gla-domainless factor xa complexed with the anticoagulant protein napc2 from the hematophagous nematode ancylostoma caninum. | napc2, an anticoagulant protein from the hematophagous nematode ancylostoma caninum evaluated in phase-ii/iia clinical trials, inhibits the extrinsic blood coagulation pathway by a two step mechanism, initially interacting with the hitherto uncharacterized factor xa exosite involved in macromolecular recognition and subsequently inhibiting factor viia (k(i)=8.4 pm) of the factor viia/tissue factor complex. napc2 is highly flexible, becoming partially ordered and undergoing significant structural ... | 2007 | 17173931 |
| development and characterization of microsatellite markers for the canine hookworm, ancylostoma caninum. | microsatellites are repetitive genomic elements that show high levels of variation and therefore provide excellent tools to study the genetics of eukaryotic organisms. hookworms are extremely common and important nematode parasites of humans and animals, causing potentially serious disease morbidity. control of hookworms in dogs is achieved by frequent treatment with anthelmintics, and in humans, anthelmintics are frequently administered in a mass-treatment community-wide approach. understanding ... | 2007 | 17136387 |
| [prevalence of helminths in dogs in the region of van and their potential public health significance]. | this study was performed in order to determine the prevalence of helminth infections among dogs and to emphasize the importance of this concept for public health. all selected dogs were from the city of van and a total of 115 fecal samples were examined. of these, 61 were from stray dogs, 26 were breeders and the final 28 dogs were dogs belonging to institutions. all samples were examined in the laboratory of the parasitology laboratory of the faculty of veterinary medicine, yuzuncu yil universi ... | 2006 | 17124658 |
| saposin-like proteins from the intestine of the blood-feeding hookworm, ancylostoma caninum. | hookworms feed on blood, utilizing haemoglobin for nutrition, growth and reproduction. the haemoglobin digestion cascade has been partially elucidated, but the process immediately preceding this event, haemolysis, has received considerably less attention. we have cloned and expressed ancylostoma caninum mrnas encoding 2 proteins belonging to the saposin-like protein (saplip) family, termed ac-slp-1 and ac-slp-2. the open reading frames of slp-1 and slp-2 were used to identify expressed sequence ... | 2007 | 17109779 |
| protein profile analysis from advanced third-stage larvae (advl3) and adult worms of gnathostoma binucleatum (nematoda: spirurida). | proteins from crude extracts of advanced third-stage larvae and adult gnathostoma binucleatum nematode worms showed protein profiles in sds-page analysis similar to echinococcus granulosus, trichinella spiralis, dipylidium caninum, ancylostoma caninum, ascaris lumbricoides and toxocara canis. the immunoblot analysis of the human serum infected or suspected to be infected with g. binucleatum using the total larvae extract recognized the 40, 60, 80 and 115 kda proteins and using the total adult wo ... | 2007 | 17096141 |
| hookworm-provoked ige-mediated pathology: capricious damage or remarkable strategy? | the existence of the canine hookworm ancylostoma caninum in the human gut seems to be curtailed by an effective ige-mediated inflammatory response against the helminth's feeding secretions, which, by damaging the host, compromises the parasite's feeding opportunities. in contrast, immunity does not develop against adapted, anthropophilic hookworm species, and it is suggested this relates to modulation of the inflammatory response by hookworm secretions. here, john croese discusses this hypothesi ... | 1998 | 17040701 |
| high-level pyrantel resistance in the hookworm ancylostoma caninum. | while anthelmintic resistance is now a widely recognized issue in the livestock industries, its existence within companion animal medicine has been rarely established conclusively. we undertook a placebo-controlled in vivo trial to measure the efficacy of pyrantel embonate against pooled isolates of the hookworm ancylostoma caninum from brisbane, australia. a statistically significant fall in adult worm burden was observed among dogs in the pyrantel treatment group compared to the control dogs ( ... | 2007 | 17011128 |
| confirmation of sex-specific transcripts from ancylostoma caninum adult worms by semi-quantitative rt-pcr. | in a previous work ancylostoma caninum sex-specific genes had being identified, however without confirmation, by using an non specific rt-pcr comparing male and female cdna. in this work more fragments had been identified and a semi-quantitative rt-pcr carried out in order to confirm the sex-specific character of the transcripts obtained. four fragments were confirmed as sex-specific or as being expressed more in one sex than the other. | 2007 | 17005221 |
| [parasitic dead-end: update]. | parasitic dead-ends occur when a parasite is unable to establish a permanent interaction in an unnatural host. although the likelihood of successful reproduction by the pathogenic agent is nul, parasitic dead-end heralds capture of new parasites and therefore expansion of the host range. angiostrongyliasis due to a. cantonensis or a. costaricensis, anisakiasis, ancylostoma caninum infection, gnathostomiasis and sparganosis are undoubtedly emerging zoonoses of particular medical interest. prevent ... | 2006 | 16999036 |
| canine faecal contamination and parasitic risk in the city of naples (southern italy). | dogs are associated with more than 60 zoonotic diseases among which, parasitosis and, in particular, helminthosis, can pose serious public-health concerns worldwide. many canine gastrointestinal parasites eliminate their dispersion elements (eggs, larvae, oocysts) by the faecal route. the quantity of canine faeces deposited on public and private property in cities worldwide is both a perennial nuisance and an important health issue. public sites such as playgrounds, parks, gardens, public square ... | 2006 | 16995934 |
| [the occurrence of intestinal nematodes in red foxes in the western pomerania]. | in the period from november 2004 to march 2005 the prevalence of gastro-intestinal nematodes of 165 foxes (vulpes vulpes l.) from the pomerania region was examined. these parasites were found in 66.1% of examined foxes. toxocara canis was the most frequent parasite and was observed in 33.9% of examined animals. toxascaris leonina was found in 1.2%, uncinaria stenocephala in 25.5%, ancylostoma caninum in 6.7 and trichuris vulpis in 10.3% of the examined foxes. | 2005 | 16913531 |
| progressive tissue injury in burns is reduced by rnapc2. | burn wounds are characterised by central necrosis surrounded by an area of stasis with compromised perfusion. secondary aggravation of the burn wound due to ischaemia in the zone of stasis can also result in necrosis. this study aims to improve circulation in the zone of stasis by reducing microthrombus formation and thereby to reduce secondary aggravation. | 2006 | 16905262 |
| a study on intestinal helminthes of dogs, foxes and jackals in the western part of iran. | human infection especially with helminth parasites is an emerging health issue, as the human environment is increasingly shared with infected animals, either pets or wild life. in this survey, the intestinal content of 83 stray dogs, 22 red foxes and 10 golden jackals collected from the west azarbaijan, kordestan and kermanshah provinces in the west of iran, were studied for the presence of helminth parasites. the percentage of different species recovered from these animals is listed as follows: ... | 2006 | 16899340 |
| [soil contamination with toxocara spp. eggs in the elblag area]. | the distribution of toxocara spp. eggs in elbl4g was studied. out of 72 soil samples collected in public places of the city 13.9% were positive and the mean egg density was 3.75/100g soil. the city backyards were much more contaminated with toxocara spp. eggs (18.0%) than the playgrounds (4.5%). in sandpits examined the eggs were not found. almost 80% of toxocara spp. eggs recovered were infective. t. cati eggs were more frequent than t. canis eggs. additionally in examined samples two eggs of a ... | 2001 | 16888965 |
| geographical information systems and canine faecal contamination: the experience in the city of naples (southern italy). | geographical information systems (gis) can be used to make transect sampling when non-moving objects are to be counted, involving choosing a line or series of lines along which the counts are to take place. this approach has been used by us in order to study canine faecal contamination in the city of naples (southern italy), and to evaluate the consequent presence of canine parasitic elements. a gis was constructed utilizing the geo-referenced digital photographs and the cadastral maps of naples ... | 2006 | 16881413 |
| gastrointestinal helminths of free-ranging florida panthers (puma concolor coryi) and the efficacy of the current anthelmintic treatment protocol. | thirty-five florida panthers (puma concolor coryi [bangs, 1899]) collected from six counties in southern florida between 1978 and 2003 were examined at necropsy for gastrointestinal helminths. the panthers were placed into two groups: 1) treated with anthelmintics (n = 17), and 2) untreated (n = 18). nine species of helminths (one trematode, six nematodes, and two cestodes) were identified in the untreated panthers. the most prevalent helminths were alaria marcianae (larue, 1917) (100%), spirome ... | 2006 | 16870865 |
| [construction and expression of protein self-splicing prokaryotic expression vector ptwin1- acapc2]. | to express recombinant ancylostoma caninum anticoagulant peptide-c2 (acapc2), a whole cdna fragment encoding acapc2 was achieved by ligation- pcr and inserted into prokaryotic expression vector ptwin1 for constructing the specific self-splicing prokaryotic expression vector, ptwin1-acapc2; positive recombinants were transformed into e. coli er2566 for expression research. the recombinant protein, acapc2-intein2-cbd, was soluble and expressed in e. coli er2566 (about 30.1% fusion protein in total ... | 2006 | 16856404 |
| endotoxaemia induces resistance to activated protein c in healthy humans. | systemic inflammation activates the tissue factor/factor viia complex (tf/fviia), leading to a procoagulant state, which may be enhanced by impairment of physiological anticoagulant pathways, such as the protein c system. besides impaired protein c activation, resistance to activated protein c (apc) may occur. we studied the effect of endotoxemia on apc resistance, analysed its determinants and evaluated the effect of tf/fviia inhibition on endotoxin-induced apc resistance. sixteen healthy male ... | 2006 | 16846480 |
| prevalence of helminthes in adult dogs in heilongjiang province, the people's republic of china. | the prevalence of helminthes in adult dogs was investigated in heilongjiang province, the people's republic of china, between 1996 and 2004. a total of 178 adult farm dogs from representative geographical locations in heilongjiang province were killed and examined for the presence of helminthes using a helminthological approach. the worms were examined, counted, and identified to species according to existing keys and descriptions. a total of 17 species of helminthes were found to infect dogs, a ... | 2006 | 16715234 |
| local activation of the tissue factor-factor viia pathway in patients with pneumonia and the effect of inhibition of this pathway in murine pneumococcal pneumonia. | the tissue factor (tf)-factor viia (fviia) complex not only is essential for activation of blood coagulation but also affect the inflammatory response during sepsis. the objective of this study was to determine the role of tf-fviia in pneumonia caused by streptococcus pneumoniae, the most important causative organism in community-acquired pneumonia and a major cause of sepsis. | 2006 | 16625114 |
| helminths of red foxes (vulpes vulpes) in denmark. | an epidemiological study of helminths in 1040 red foxes collected from various localities in denmark during 1997-2002, revealed 21 helminth species at autopsy, including nine nematode species: capillaria plica (prevalence 80.5%), capillaria aerophila (74.1%), crenosoma vulpis (17.4%), angiostrongylus vasorum (48.6% from northern zealand (endemic area)), toxocara canis (59.4%), toxascaris leonina (0.6%), uncinaria stenocephala (68.6%), ancylostoma caninum (0.6%), and trichuris vulpis (0.5%); seve ... | 2006 | 16580775 |
| the ever-expanding neuropeptide gene families in the nematode caenorhabditis elegans. | neuropeptides act as chemical signals in the nervous system to modulate behaviour. with the ongoing est projects and dna sequence determination of different genomes, the identification of neuropeptide genes has been made easier. despite the relatively 'simple' repertoire of behaviours in the nematode caenorhabditis elegans, this worm contains a surprisingly large and diverse set of neuropeptide genes. at least 109 genes encoding over 250 potential neuropeptides have been identified in c. elegans ... | 2005 | 16569285 |
| a survey of the intestinal transcriptomes of the hookworms, necator americanus and ancylostoma caninum, using tissues isolated by laser microdissection microscopy. | the gastrointestinal tracts of multi-cellular blood-feeding parasites are targets for vaccines and drugs. recently, recombinant vaccines that interrupt the digestion of blood in the hookworm gut have shown efficacy, so we explored the intestinal transcriptomes of the human and canine hookworms, necator americanus and ancylostoma caninum, respectively. we used laser microdissection microscopy to dissect gut tissue from the parasites, extracted the rna and generated cdna libraries. a total of 480 ... | 2006 | 16545815 |
| prevalence of zoonotic agents in dogs visiting hospitalized people in ontario: implications for infection control. | visitation of hospitalized people by dogs is becoming commonplace, but little is known about the potential health risks of introducing dogs to healthcare settings. this cross-sectional study evaluated the prevalence of zoonotic agents in a group of 102 visitation dogs from a variety of sources across ontario. between may and july 2004, owners were interviewed by a standardized questionnaire while dogs underwent a standardized physical examination. one specimen of faeces, hair-coat brushings and ... | 2006 | 16466831 |
| intestinal macro- and microparasites of wolves (canis lupus l.) from north-eastern poland recovered by coprological study. | wolf scats collected during ecological studies in mazury lake district in ne poland were analysed for intestinal micro- and macroparasites. five nematode species were identified: ancylostoma caninum (ercolani, 1859), uncinaria stenocephala (railliet, 1884), trichuris vulpis (froelich, 1789), toxocara canis (werner, 1782) and toxascaris leonina (von linstow, 1902). among cestode species there were identified infections with dipylidium caninum (linnaeus, 1785). the overall helminth prevalence was ... | 2005 | 16457480 |
| the dingo non-long terminal repeat retrotransposons from the genome of the hookworm, ancylostoma caninum. | members of the retrotransposable element (rte) clade of non-long terminal repeat (ltr) retrotransposon are widely distributed among eukaryote taxa, with representatives known from caenorhabditis elegans, mammals, mosquitoes, schistosomes, and other taxa. an rte retrotransposon has not, however, been characterized in detail from a parasitic nematode. here, we characterize two discrete copies of an rte-like non-ltr retrotransposon from the genome of the dog hookworm, ancylostoma caninum. the eleme ... | 2006 | 16445914 |
| ancylostoma caninum mtp-1, an astacin-like metalloprotease secreted by infective hookworm larvae, is involved in tissue migration. | infective larvae (l3) of nematodes secrete macromolecules that are critical to infection and establishment of the parasite in the host. the dog hookworm ancylostoma caninum secretes an astacin-like metalloprotease, ac-mtp-1, upon activation in vitro with host serum. recombinant ac-mtp-1 was expressed in the baculovirus/insect cell system as a secreted protein and was purified from culture medium by two separate methods, cation-exchange fast-performance liquid chromatography and gelatin-affinity ... | 2006 | 16428741 |
| an epidemiological study of gastrointestinal parasites of dogs from southern greater buenos aires (argentina): age, gender, breed, mixed infections, and seasonal and spatial patterns. | a total of 2193 fecal samples from owned dogs were collected during the 2003-2004 period in southern greater buenos aires, and were evaluated for the presence of intestinal parasites by a flotation-centrifugation method. the overall prevalence was 52.4%, and the 11 species found were: ancylostoma caninum (13%), isospora ohioensis complex (12%), toxocara canis (11%), trichuris vulpis (10%), sarcocystis sp. (10%), giardia duodenalis (9%), isospora canis (3%), hammondia-neospora complex (3%), dipil ... | 2006 | 16364551 |
| molecular cloning and functional characterization of an aspartic protease from the hard tick haemaphysalis longicornis. | haemaphysalis longicornis cdna encoding an aspartic protease (longepsin) was identified from a midgut cdna library. the longepsin cdna contains 1176bp that code for 392 amino acid residues with a predictable molecular weight of 39.3kda. the cdna has a signal peptide sequence associated with the n-terminal domains and domain structure analysis revealed that the deduced protein has two aspartic acid residues that are characteristic of a single active site for aspartic proteases. this novel longeps ... | 2006 | 16360947 |
| [prevalence of intestinal helminths in dogs from quindío province]. | intestinal helminths are pathogens for domestic animals and provide a source of potential infection for humans. | 2005 | 16276682 |
| enhancement of protein secretion in pichia pastoris by overexpression of protein disulfide isomerase. | a potential vaccine candidate, necator americanus secretory protein (na-asp1), against hookworm infections, has been expressed in pichia pastoris. na-asp1, a 45 kda protein containing 20 cysteines, was directed outside the cell by fusing the protein to the preprosequence of the alpha-mating factor of saccharomyces cerevisiae. most of the protein produced by single copy clones was secreted outside the cell. however, increasing gene copy number of na-asp1 protein in p. pastoris saturated secretory ... | 2006 | 16255058 |
| vaccination with recombinant aspartic hemoglobinase reduces parasite load and blood loss after hookworm infection in dogs. | hookworms infect 730 million people in developing countries where they are a leading cause of intestinal blood loss and iron-deficiency anemia. at the site of attachment to the host, adult hookworms ingest blood and lyse the erythrocytes to release hemoglobin. the parasites subsequently digest hemoglobin in their intestines using a cascade of proteolysis that begins with the ancylostoma caninum aspartic protease 1, apr-1. | 2005 | 16231975 |
| biochemical characterization and vaccine potential of a heme-binding glutathione transferase from the adult hookworm ancylostoma caninum. | we report the cloning and expression of ac-gst-1, a novel glutathione s-transferase from the adult hookworm ancylostoma caninum, and its possible role in parasite blood feeding and as a vaccine target. the predicted ac-gst-1 open reading frame contains 207 amino acids (mass, 24 kda) and exhibited up to 65% amino acid identity with other nematode gsts. mrna encoding ac-gst-1 was detected in adults, eggs, and larval stages, but the protein was detected only in adult hookworm somatic extracts and e ... | 2005 | 16177370 |
| vaccination with irradiated ancylostoma caninum third stage larvae induces a th2 protective response in dogs. | while x-irradiated live parasites are not an acceptable proposition for human vaccination, they offer a ready experimental system to explore mechanisms by which immunity against hookworm infection may be induced in humans. as such, we sought to further elucidate the details of this highly protective immune response induced by the irradiated vaccine in canids, with special emphasis on the cellular aspects of the response. vaccination with irradiated l3 induced high production of antibodies and st ... | 2006 | 16140437 |
| cloning and characterisation of genes encoding two transforming growth factor-beta-like ligands from the hookworm, ancylostoma caninum. | to elucidate the role of transforming growth factor beta (tgf-beta) signalling in the arrest/reactivation pathway of the ancylostoma caninum hookworm, two parasite-encoded tgf-beta-like ligands were cloned and characterised. ac-dbl-1 showed 60% amino acid identity to the caenorhabditis elegansdbl-1 gene, which regulates growth while ac-daf-7 showed 46% amino acid identity to ce-daf-7 which regulates arrested development. exon/intron organisation of the genes for ac-dbl-1 and ac-daf-7 were differ ... | 2005 | 16140304 |
| identification of a daf-7 ortholog from the hookworm ancylostoma caninum. | infective hookworm l3 encounter a host specific signal during invasion that re-activates suspended developmental pathways. response to this cue is critical for the successful infection and completion of the life cycle in the host. in the free-living nematode caenorhabditis elegans, recovery from the developmentally arrested dauer stage in response to environmental cues is analogous to the resumption of development in invading hookworm l3. transforming growth factor beta (tgf-beta) and insulin-li ... | 2005 | 16135366 |
| isolation of anthelmintic resistant ancylostoma caninum. | 1987 | 16031355 | |
| evaluation of endogenous reference genes for real-time pcr quantification of gene expression in ancylostoma caninum. | 2005 | 15993958 | |
| gene expression profiles associated with the transition to parasitism in ancylostoma caninum larvae. | ancylostoma caninum is a common canine parasite responsible for anemia and death in infected dogs. gene expression profiling was used to investigate molecular differences between two different forms of the third larval stage (l3s): infective free-living larvae and in vitro serum-stimulated larvae that mimic the initial stages of parasitism of a host. we developed an a. caninum cdna microarray consisting of 4191 est clones, and used it to identify a set of 113 genes that are differentially regula ... | 2005 | 15979737 |
| cloning and characterization of a novel immunogenic protein 3 (nip3) from brugia malayi by immuno screening of a phage-display cdna expression library. | iterative screening of a phage display cdna expression library of the third-stage larvae (l3) of brugia malayi with sera from putatively immune individuals (endemic normal, en) identified a novel clone with insert showing significant homology to onchocerca volvulus novel immunogenic protein-3 (ov-nip3) gene and caenorhabditis elegans nip3-like protein and hence the gene was designated brugia malayi nip3-like protein (bmnip3). est database analysis showed that ests from several gastrointestinal n ... | 2005 | 15952042 |
| rag1 core and v(d)j recombination signal sequences were derived from transib transposons. | the v(d)j recombination reaction in jawed vertebrates is catalyzed by the rag1 and rag2 proteins, which are believed to have emerged approximately 500 million years ago from transposon-encoded proteins. yet no transposase sequence similar to rag1 or rag2 has been found. here we show that the approximately 600-amino acid "core" region of rag1 required for its catalytic activity is significantly similar to the transposase encoded by dna transposons that belong to the transib superfamily. this supe ... | 2005 | 15898832 |
| new oral linguiform projections and their associated neurons in the third-stage infective larva of the parasitic nematode oesophagostomum dentatum. | the infective larvae (l3i) of the nematode parasite of swine, oesophagostomum dentatum, are passively ingested by their hosts. the l3i exhibit certain behaviors that are probably selected to increase the likelihood of ingestion, by strategic positioning in the environment. the larvae show positive geotactic behavior and respond to temperature variations in their environment, as shown by their behavior on a thermal gradient. to investigate neuronal control of this behavior, we initiated a study o ... | 2005 | 15856873 |
| investigating hookworm genomes by comparative analysis of two ancylostoma species. | hookworms, infecting over one billion people, are the mostly closely related major human parasites to the model nematode caenorhabditis elegans. applying genomics techniques to these species, we analyzed 3,840 and 3,149 genes from ancylostoma caninum and a. ceylanicum. | 2005 | 15854223 |
| inactivation of cd11b in a mouse transgenic model protects against sepsis-induced lung pmn infiltration and vascular injury. | to inactivate chronically the beta2-integrin cd11b (mac-1), we made a transgenic model in mice in which we expressed the cd11b antagonist polypeptide neutrophil inhibitory factor (nif). using these mice, we determined the in vivo effects of cd11b inactivation on polymorphonuclear leukocyte (pmn) function and acute lung injury (ali) induced by escherichia coli septicemia. in wild-type pmns, cd11b expression was induced within 1 h after e. coli challenge, whereas this response was significantly re ... | 2005 | 15831844 |
| liver phosphatases in mice treated with lead during murine ancylostomiasis. | both infective larvae and pollutants disturb the homeostasis of female swiss albino mice, causing ancylostomiasis and severe immunophysiological changes. in our experimental design, mice in group a were fed 0.01 mg of lead nitrate before infection with 500 larvae of ancylostoma caninum, mice in group b were infected with larvae without previous lead treatment, and group c received lead nitrate treatment alone. control animals in group d were neither treated with lead nitrate nor infected with la ... | 2005 | 15814320 |
| tribendimidine: a promising, safe and broad-spectrum anthelmintic agent from china. | we review, for the first time, a 20-year chinese story of research and development pertaining to tribendimidine, a promising anthelmintic agent that is safe and exhibits a broad spectrum of activity. tribendimidine was first synthesized at the national institute of parasitic diseases in shanghai in the mid 1980s. in laboratory studies, tribendimidine showed high efficacy against nippostrongylus braziliensis in rats, necator americanus in hamsters, ancylostoma caninum and toxocara canis in dogs, ... | 2005 | 15777691 |
| cloning and characterisation of an aspartyl protease inhibitor (api-1) from ancylostoma hookworms. | hookworm infection persists as a public health problem in developing nations. vaccine-based strategies offer the best chance of long-term control. aspartyl protease inhibitors from parasitic nematodes are highly immunogenic, and have been suggested as potential vaccine antigens. an aspartyl protease inhibitor, api-1, was cloned and characterised from the hookworms ancylostoma caninum and ancylostoma ceylanicum. using sequence from the hookworm expressed sequence tag project, specific primers wer ... | 2005 | 15722082 |
| molecular analysis of astacin-like metalloproteases of ostertagia ostertagi. | in this study, we describe the molecular analysis of zinc-metalloproteases from the abomasal nematode ostertagia ostertagi which were exclusively recognized by local antibodies of immune cattle. full-length or partial coding sequences of 4 different zinc-metalloprotease cdnas of ostertagia (met-1, -2, -3 and -4) were amplified using gene-specific primers using the 3'- and 5'-rapid amplification of cdna ends (race) technique. sequence analysis identified the cdnas as encoding zinc-metalloprotease ... | 2005 | 15700760 |
| ecological analysis and description of the intestinal helminths present in dogs in mexico city. | the objective of the present study was to determine the prevalence of parasitoses in stray dogs from mexico city, as well as to describe the structure of the intestinal helminth communities present in said hosts. one hundred and twenty dog intestines were collected and dissected to evaluate their parasitic load; finding parasites in 102 dogs (85%). the cestodes collected were: dipylidium caninum in 72 dogs (60%), taenia hydatigena in three (2.5%), taenia pisiformis in two (1.6%), echinococcus gr ... | 2004 | 15631907 |
| infective larvae of the human hookworms necator americanus and ancylostoma duodenale differ in their orientation behaviour when crawling on surfaces. | the infective third stage larvae of hookworms infect their hosts by active skin invasion, and they find and recognize their hosts by the behavioural phases of activation, directed crawling, and penetration. here we analyse the orientation of the infective larvae of the human hookworms necator americanus and ancylostoma duodenale when crawling on surfaces. their behaviour differed from that of the larvae of the dog hookworm ancylostoma caninum, but the two species also differed from each other. n ... | 2004 | 15614586 |
| identification of specific male and female genes in adult ancylostoma caninum. | the hookworm ancylostoma canium represents a serious health problem, not only for animals but also for humans. these blood-feeding parasites produce various proteolytic enzymes in order to digest the host hemoglobin. the female worm ingests more blood than does the male. it is not known whether this difference is accompanied by expression of sex-specific proteinases. the identification of new genes related either to the developmental process of maturation of each sex or to the proteinases secret ... | 2004 | 15604493 |
| amphidial neurons adl and ash initiate sodium dodecyl sulphate avoidance responses in the infective larva of the dog hookworm anclyostoma caninum. | ablations of specific amphidial neuron pairs with a laser microbeam were conducted to understand better the neurological basis of the behaviours of larval parasitic nematodes. to date, the functions of the amphidial neurons of caenorhabditis elegans and their counterparts in parasitic nematodes have been found to be remarkably conserved allowing the possibility to predict the relationships between neurons and their functions. therefore, we anticipated that ablation of neuron pairs ash and ask wo ... | 2004 | 15542093 |
| application of a species-specific pcr-rflp to identify ancylostoma eggs directly from canine faeces. | apart from their veterinary importance, the hookworms ancylostoma caninum, ancylostoma braziliense and ancylostoma caninum are also capable of causing zoonotic disease in humans. a highly sensitive and species-specific pcr-rflp technique was utilised to detect and differentiate the various canine ancylostoma spp directly from eggs in faeces. this technique was utilised to screen 101 canine faecal samples from parasite endemic tea growing communities in assam, india, as part as an ongoing epidemi ... | 2004 | 15325050 |
| ac-saa-1, an immunodominant 16 kda surface-associated antigen of infective larvae and adults of ancylostoma caninum. | a cdna encoding a surface-associated antigen was cloned from an ancylostoma caninum infective larvae (l(3)) cdna library by immunoscreening with pooled human immune sera. the sera were obtained from individuals living in an ancylostoma duodenale hookworm-endemic region of china, who had light intensity infections and high antibody titers against a. caninum l(3). ancylostoma caninum surface-associated antigen-1 is encoded by an 843 bp mrna with a predicted open reading frame of 162 amino acids. r ... | 2004 | 15313130 |
| a pore-forming haemolysin from the hookworm, ancylostoma caninum. | hookworms feed on blood, but the mechanism by which they lyse ingested erythrocytes is unknown. here we show that ancylostoma caninum, the common dog hookworm, expresses a detergent soluble, haemolytic factor. activity was identified in both adult and larval stages, was heat-stable and unaffected by the addition of protease inhibitors, metal ions, chelators and reducing agents. trypsin ablated lysis indicating that the haemolysin is a protein. a closely migrating doublet of hookworm proteins wit ... | 2004 | 15313129 |
| ancylostoma ceylanicum anticoagulant peptide-1: role of the predicted reactive site amino acid in mediating inhibition of coagulation factors xa and viia. | hookworm infection is a leading cause of gastrointestinal blood loss and iron deficiency anemia in developing countries. ancylostoma hookworms secrete potent anticoagulants, which have been shown to target coagulation factors xa and the factor viia/tissue factor complex, respectively. the goal of these experiments was to determine the mechanism of action of three recombinant hookworm anticoagulants using in vitro assays. three hookworm coagulation inhibitors were expressed and purified, along wi ... | 2004 | 15279961 |
| intestinal obstruction by eosinophilic jejunitis. | eosinophilic enteritis is an uncommon disease that rarely develops as a surgical emergency. although it may be associated with infestation by ancylostoma caninum, its etiology is unknown and often related to a personal or family history of atopy. a transmural involvement may cause intestinal obstruction--more frequently in the jejunum--or even acute abdomen, which may or may not be accompanied by intestinal perforation. the latter two conditions tend to be more commonly associated with ileum dis ... | 2004 | 15259143 |
| phosphoinositide-3-oh-kinase inhibitor ly294002 prevents activation of ancylostoma caninum and ancylostoma ceylanicum third-stage infective larvae. | the developmentally arrested hookworm infective larva resumes development only after encountering specific host-mediated cues during invasion. these cues activate a signaling pathway that culminates in the resumption of development. in ancylostoma caninum, activation is characterised by the resumption of feeding and the release of excretory/secretory products required for infection. the dauer stage of the free-living nematode caenorhabditis elegans is a developmentally arrested stage analogous t ... | 2004 | 15217729 |
| a multi-enzyme cascade of hemoglobin proteolysis in the intestine of blood-feeding hookworms. | blood-feeding pathogens digest hemoglobin (hb) as a source of nutrition, but little is known about this process in multicellular parasites. the intestinal brush border membrane of the canine hookworm, ancylostoma caninum, contains aspartic proteases (apr-1), cysteine proteases (cp-2), and metalloproteases (mep-1), the first of which is known to digest hb. we now show that hb is degraded by a multi-enzyme, synergistic cascade of proteolysis. recombinant apr-1 and cp-2, but not mep-1, digested nat ... | 2004 | 15199048 |
| inhibition of the tissue factor/factor viia pathway does not influence the inflammatory or antibacterial response to abdominal sepsis induced by escherichia coli in mice. | anticoagulants have gained increasing attention for the treatment of sepsis. inhibition of the tissue factor (tf)/factor (f) viia pathway has been shown to attenuate the activation of coagulation and to prevent death in a primate model of sepsis caused by gram-negative bacteria. | 2004 | 15181580 |
| persistent efficacy of moxidectin canine sustained-release injectable against experimental infections of ancylostoma caninum and uncinaria stenocephala in dogs. | the efficacy of moxidectin canine sustained-release injectable administered at fixed intervals before administration of an oral hookworm challenge was evaluated in 40 laboratory dogs. groups of eight dogs were treated with the moxidectin sustained-release formulation by sc injection approximately 3, 4, 5, or 6 months before being given oral inoculations with 300 ancylostoma caninum larvae on day 0 and 400 uncinaria stenocephala larvae one day later. dogs were euthanized 21 days after parasite in ... | 2003 | 15136983 |
| vaccination of dogs with a recombinant cysteine protease from the intestine of canine hookworms diminishes the fecundity and growth of worms. | we expressed a catalytically active cysteine protease, ac-cp-2, from the blood-feeding stage of the canine hookworm ancylostoma caninum and vaccinated dogs with the purified protease. dogs acquired high-titer, antigen-specific antibody responses, and adult hookworms recovered from the intestines of vaccinated dogs were significantly smaller than hookworms from control dogs. there was also a marked decrease in fecal egg counts and the number of female hookworms in vaccinated dogs. ac-cp-2 is expr ... | 2004 | 15122534 |
| rnapc2. nuvelo. | recombinant nematode anticoagulant protein-2 (rnapc2) is an antithrombotic protein under development by nuvelo as a potential therapeutic agent for the treatment of major acute cardiovascular disease such as unstable angina and myocardial infarction. phase ii clinical trials were ongoing in january 2004, although development for deep vein thrombosis had been suspended by dendreon due to a requirement for a partner to assist with additional phase ii clinical trials. | 2004 | 15083600 |
| prevalence of dogs with intestinal parasites in tochigi, japan in 1979, 1991 and 2002. | a total of 772 canine fecal specimens were collected in tochigi in 1979, 1991 and 2001. the frequency of dogs that were kept indoor in 2002 (72.8%) was significantly higher than that for 1979 (21.2%, p < 0.001) and 1991 (40.6%, p < 0.01). the frequency of parasitized dogs in 2002 (18.8%) was significantly lower than that for 1979 (41.9%, p < 0.01). the findings of this study confirm the declining trend in japan in the prevalence of helminth parasites in dogs, particularly ancylostoma caninum and ... | 2004 | 15041099 |
| parasitic helminths of black bear cubs (ursus americanus) from florida. | twenty-two florida black bear (ursus americanus floridanus) cubs (< or = 12 mo old) from florida were examined for endoparasites between 1998 and 2003. eleven species of helminths were found (8 nematodes, 2 trematodes, and 1 acanthocephalan). the most prevalent helminths were ancylostoma caninum (64%), macracanthorhynchus ingens (36%), strongyloides sp. (27%), baylisascaris transfuga (23%), and a. tubaeforme (14%). new host records include a. tubaeforme and brachylaima virginianum. this is the f ... | 2004 | 15040687 |
| investigations of hookworm larvae. i. on the phototropism of infective larvae of ancylostoma caninum. | 1952 | 14951409 | |
| infections of ancylostoma caninum in abnormal hosts. | 1952 | 14928159 | |
| recombinant factor viia as an antidote for anticoagulant treatment. | increasing knowledge on the function of the hemostatic system in vivo and limitations of currently available anticoagulant agents have led to the development of a new generation of anticoagulants. these new agents have a greater specificity towards activated coagulation pathways and factors and are presently being evaluated in clinical studies. the new generation anticoagulants include specific inhibitors of factor iia (melagatran), factor xa (pentasaccharides), and agents that interfere with ti ... | 2004 | 14872424 |
| chemical control of larvae of the dog hookworm ancylostoma caninum (ercolani). | 1951 | 14819522 | |
| recombinant nematode anticoagulant protein c2, an inhibitor of tissue factor/factor viia, attenuates coagulation and the interleukin-10 response in human endotoxemia. | the tissue factor-factor (f)viia complex (tf/fviia) is responsible for the initiation of blood coagulation under both physiological and pathological conditions. recombinant nematode anticoagulant protein c2 (rnapc2) is a potent inhibitor of tf/fviia, mechanistically distinct from tissue factor pathway inhibitor. the first aim of this study was to elucidate the pharmacokinetics and pharmacodynamics of a single intravenous (i.v.) dose of rnapc2. the second aim was to study its effect on endotoxin- ... | 2004 | 14717968 |
| a serologically diagnosed human case of cutaneous larva migrans caused by ancylostoma caninum. | a 15-year-old boy, who had recently arrived back from a trip to cambodia for a missionary camp, presented with several serpiginous thread-like skin lesions that began as small papules on the left upper extremities 2 weeks before his visit to hospital. the skin lesions were pruritic and erythematous, and had migrated to the chest and abdomen. the histopathological findings showed only lymphocytic and eosinophilic infiltrations in the dermis of the biopsied skin lesion. the patient's serum reacted ... | 2003 | 14699264 |
| anticoagulant peptides from ancylostoma caninum are immunologically distinct and localize to separate structures within the adult hookworm. | 2004 | 14698444 | |
| treatment of ebola virus infection with a recombinant inhibitor of factor viia/tissue factor: a study in rhesus monkeys. | infection with the ebola virus induces overexpression of the procoagulant tissue factor in primate monocytes and macrophages, suggesting that inhibition of the tissue-factor pathway could ameliorate the effects of ebola haemorrhagic fever. here, we tested the notion that blockade of fviia/tissue factor is beneficial after infection with ebola virus. | 2003 | 14683653 |
| virology. new vaccine and treatment excite ebola researchers. | 2003 | 14615510 | |
| pharmacokinetics and anticoagulant properties of the factor viia-tissue factor inhibitor recombinant nematode anticoagulant protein c2 following subcutaneous administration in man. dependence on the stoichiometric binding to circulating factor x. | recombinant nematode anticoagulant protein c2 (rnapc2) is a potent (k(i) =10 pm), inhibitor of the factor viia/tissue factor (fviia/tf) complex that requires the prerequisite binding to zymogen or activated factor x (fx). in two double blind, place-bo-controlled, sequential dose-escalation phase i studies, rnapc2 was found to be safe and well tolerated following single and repeat subcutaneous administrations in healthy human male volunteers at doses ranging from 0.3 to 5 micro g/kg. there was a ... | 2003 | 14597974 |
| [biology and physiopathology of tissue factor and its relevance for cardiovascular diseases]. | tissue factor (tf) is a transmembrane glycoprotein, currently considered as being the major regulator of the coagulation cascade and the initiator of thrombogenesis in vivo. when tf comes in contact with blood, it forms a high-affinity complex with factors vii/viia, activating factors ix and x and thus leading to the formation of an insoluble fibrin clot. the regulation of tf-viia activity plays a key role in blood-vessel wall interactions. selective patterns of cellular expression of tf are obs ... | 2003 | 14558284 |
| haemonchus contortus: molecular characterisation of a small heat shock protein. | a cdna encoding a predicted small heat shock protein, hsp20, was isolated from the parasitic nematode haemonchus contortus. this cdna encoded a predicted protein of 156 amino acids, which had high sequence identity with other nematode small heat shock proteins. southern blot analysis of genomic dna suggested that in h. contortus hsp20 is encoded by a single copy gene. the hsp20 transcript and protein were expressed in the infective larvae (l3), early l4 and adult stages, but expression was not i ... | 2003 | 14552856 |
| ecological analyses of the intestinal helminth communities of the wolf, canis lupus, in spain. | this work describes the ecological characteristics of the intestinal helminth communities of 50 wolves (canis lupus l.) from spain. the species found were classified into three groups according to prevalence, intensity and intestinal distribution. taenia hydatigena pallas, 1766 and uncinaria stenocephala (railliet, 1884) are the core species of the community. taenia multiceps (leske, 1780) is a secondary species. the rest of the species, alaria alata (goeze, 1782), taenia serialis (gervais, 1847 ... | 2003 | 14535350 |
| effect of vaccination with a recombinant fusion protein encoding an astacinlike metalloprotease (mtp-1) secreted by host-stimulated ancylostoma caninum third-stage infective larvae. | laboratory dogs were vaccinated intramuscularly with a recombinant fusion protein (expressed and isolated from escherichia coli) formulated with the glaxo smithkline adjuvant system 02 (as02). the fusion protein encoded ac-mtp-1, a developmentally regulated astacinlike metalloprotease secreted by host-stimulated ancylostoma caninum third-stage larvae (l3). control dogs were injected intramuscularly with an equivalent amount of as02 adjuvant alone. the vaccinated and control dogs were then challe ... | 2003 | 14533704 |
| evaluation of the role of the asialoglycoprotein receptor in the clearance of uk-279,276 (recombinant neutrophil inhibitory factor). | 1. uk-279,276 or recombinant neutrophil inhibitory factor (rnif) is a glycoprotein that has been investigated as an intravenous treatment for stroke. previous data indicate that the asialoglycoprotein receptor (asgpr) in the liver might have a role in the uptake and clearance of uk-279,276 from plasma in rats and dogs. 2. biliary elimination of sialo uk-279,276 can be inhibited by the co-administration of asialo fetuin, a known substrate for asgpr. these data are in keeping with sialo uk-279,276 ... | 2003 | 14514443 |
| electroesophagogram of individual hookworm (ancylostoma caninum). | a device for recording regular rhythmic electrical phenomena that are synchronous with esophageal contractions in ancylostoma cantinum is described. | 1962 | 14493038 |
| [histochemical studies on ancylostoma caninum. (i-ii)]. | 1961 | 14477283 | |
| effects of crowding and of superinfection on habitat selection and egg production in ancylostoma caninum. | 1961 | 14459947 | |
| [anatomical studies on the development of ancylostoma caninum]. | 1961 | 14457904 | |
| in vitro studies on ancylostoma caninum with thiabendazole with observations on larval exsheathment. | 1965 | 14282469 | |
| effect of route of administration of vaccine and challenge on the immunogenic efficiency of double vaccination with irradiated ancylostoma caninum larvae. | 1965 | 14275207 | |
| studies on canine ancylostomiasis: double vaccination with x-irradiated ancylostoma caninum larvae. | 1965 | 14256948 |