Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| group a rotavirus and norovirus genotypes circulating in the northeastern brazil in the post-monovalent vaccination era. | group a rotaviruses (rva) and noroviruses (nov) are the leading cause of acute gastroenteritis (age) worldwide. childhood diarrhea deaths and hospital admissions have declined since the introduction of the monovalent (g1p[8]) vaccine (rotarix(®) [rv1]) in the national immunization program in brazil in 2006. this study aims to investigate the epidemiological profile of nov and rva infections from children with age in the northeastern region of brazil in the post vaccine season. two-hundred fecal ... | 2015 | 25982627 |
| high prevalence and diversity of bovine astroviruses in the faeces of healthy and diarrhoeic calves in south west scotland. | astroviruses (astv) are single-stranded, positive-sense rna viruses and one of the major causes of infant diarrhoea worldwide. diarrhoea is a common and important cause of morbidity and mortality in calves; therefore, we investigated whether the presence of astv is associated with calf diarrhoea. we identified diverse astv lineages from faecal samples of both healthy and diarrhoeic calves and healthy adult cattle in south west scotland. astv was common in calves (present in 74% (85/115) of sampl ... | 2015 | 25979841 |
| rotavirus genotypes and the indigenous children of brazilian midwest in the vaccine era, 2008-2012: footprints of animal genome. | world group a rotavirus (rva) surveillance data provides useful estimates of the disease burden, however, indigenous population might require special consideration. the aim of this study was to describe the results of g- and p-types from brazilian native children ≤ 3 years. furthermore, selected strains have been analyzed for the vp7, vp6, vp4, and nsp4 encoding genes in order to gain insight into genetic variability of brazilian strains. a total of 149 samples, collected during 2008-2012, were ... | 2015 | 25963945 |
| rotavirus group a genotypes detected through diarrheal disease surveillance in haiti, 2012. | samples collected in 2012 through diarrheal disease surveillance in haiti were tested for rotavirus by enzyme immunoassay and real time rt-pcr and positive samples were genotyped. the predominant genotypes were g1p[8] (29% prevalence) and g9p[8] (21%). the observed genotype prevalence was similar to that reported previously for other caribbean countries. | 2015 | 25962775 |
| comparative characteristics of the vp7 and vp4 antigenic epitopes of the rotaviruses circulating in russia (nizhny novgorod) and the rotarix and rotateq vaccines. | two live, attenuated rotavirus a (rva) vaccines, rotarix and rotateq, have been successfully introduced into national immunization programs worldwide. the parent strains of both vaccines were obtained more than 30 years ago. nonetheless, only very limited data are available on the molecular similarity of the vaccine strains and their genetic relationships to the wild-type strains circulating within the territory of russian federation. in this study, we have determined the nucleotide sequences of ... | 2015 | 25944143 |
| evolution of a g6p[6] rotavirus strain isolated from a child with acute gastroenteritis in ghana, 2012. | unusual human g6p[6] rotavirus a (rva) strains have been reported sporadically in europe and africa, but how they evolved was not fully understood. the whole genome of a ghanaian g6p[6] strain designated pml1965 (2012) was analysed to understand how it evolved in africa and to learn how its g6 vp7 gene was related to that of rotaviruses of human and artiodactyl origin. the genotype constellation of rva/human-wt/gha/pml1965/2012/g6p[6] was g6-p-[6]-i2-r2-c2-m2-a2-n2-t2-e2-h2. it shared sublineage ... | 2015 | 25934790 |
| rotavirus vaccine effectiveness in latin american and caribbean countries: a systematic review and meta-analysis. | there are two group a rotavirus (rva) vaccines available worldwide since 2006: monovalent (rotarix(®), rv1) and pentavalent (rotateq(®), rv5). currently, 16 countries and 1 territory in latin america and the caribbean (lac) have introduced rva vaccines and since their introduction several impact and effectiveness studies have been conducted in different countries. the purpose of this study was to assess rva vaccine effectiveness in lac countries. | 2015 | 25919169 |
| [the application and epidemiological research of xtag gpp multiplex pcr in the diagnosis of infectious diarrhea]. | to investigate the application value of xtag (®) gastrointestinal pathogen panel (xtag9(®) gpp) multiplex pcr in the early diagnosis of infectious diarrhea, and understand the epidemiology of intestinal diarrhea pathogens. | 2015 | 25916780 |
| genomic characterization of uncommon human g3p[6] rotavirus strains causing diarrhea in children in italy in 2009. | group a rotaviruses (rva) are the leading cause of acute gastroenteritis in young children, causing up to 450,000 deaths worldwide, mostly in developing countries. most of rva human infections in developed countries are related to five major g/p combinations: g1p[8], g2p[4], g3p[8], g4p[8] and g9p[8]. during the surveillance activity of rotanet-italy, three uncommon g3p[6] rva strains, designated as rva/human-wt/ita/na01/2009/g3p[6], rva/human-wt/ita/na06/2009/g3p[6], and rva/human-wt/ita/na19/2 ... | 2015 | 25913157 |
| etiology of acute diarrhea in tunisian children with emphasis on diarrheagenic escherichia coli: prevalence and identification of e. coli virulence markers. | diarrheal diseases can be caused by viral, bacterial and parasitic infections. this paper provides a preliminary image of diarrhea with regards to etiology and epidemiologic factors in tunisian children less than five years of age. | 0 | 25909062 |
| detection and characterization of a human g9p[4] rotavirus strain in japan. | in a surveillance system in osaka city, japan, 48 sporadic rotavirus a (rva) infections were detected during 2008/2009-2011/2012 seasons. the g/p-genotypes of detected rvas were g1p[8], g2p[4], g3p[8], g9p[4], and g9p[8]. although g9p[4] is a rare genotype that had not been reported in japan, it was the second most prevalent genotype, following g1p[8], and accounted for 35.3% of rva cases in the 2011/2012 season. further genotyping revealed that the g9p[4] strain had genotype 2 internal protein ... | 2015 | 25907907 |
| genomic characterization of g3p[6], g4p[6] and g4p[8] human rotaviruses from wuhan, china: evidence for interspecies transmission and reassortment events. | we report here the whole genomic analyses of two g4p[6] (rva/human-wt/chn/e931/2008/g4p[6], rva/human-wt/chn/r1954/2013/g4p[6]), one g3p[6] (rva/human-wt/chn/r946/2006/g3p[6]) and one g4p[8] (rva/human-wt/chn/e2484/2011/g4p[8]) group a rotavirus (rva) strains detected in sporadic cases of diarrhea in humans in the city of wuhan, china. all the four strains displayed a wa-like genotype constellation. strains e931 and r1954 shared a g4-p[6]-i1-r1-c1-m1-a8-n1-t1-e1-h1 constellation, whilst the 11 g ... | 2015 | 25891280 |
| evolution of human g4p[8] group a rotavirus strains circulating in italy in 2013. | group a rotaviruses (rva) are the leading cause of acute gastroenteritis in young (<5 years of age) children, causing up to 450.000 deaths worldwide, mostly in developing countries. vp7 (g-type) and vp4 (p-type) genotypes are the basis for the binary rva classification. although at least 27 g-types and 37 p-types of rotavirus are presently known, most rva infections in humans worldwide are associated with the five major g/p combinations g1p[8], g2p[4], g3p[8], g4p[8] and g9p[8]. during rva gastr ... | 2015 | 25889745 |
| unusual rotavirus genotypes among children with acute diarrhea in saudi arabia. | human rotavirus a (human rv-a) is the most common cause of viral gastroenteritis in infants. the objective of the study was to characterize the g and p genotypes among clinical rotavirus isolates from children with acute diarrhea admitted to a tertiary care hospital in riyadh, saudi arabia. | 2015 | 25884670 |
| structural basis for 2'-5'-oligoadenylate binding and enzyme activity of a viral rnase l antagonist. | synthesis of 2'-5'-oligoadenylates (2-5a) by oligoadenylate synthetase (oas) is an important innate cellular response that limits viral replication by activating the latent cellular rnase, rnase l, to degrade single-stranded rna. some rotaviruses and coronaviruses antagonize the oas/rnase l pathway through the activity of an encoded 2h phosphoesterase domain that cleaves 2-5a. these viral 2h phosphoesterases are phylogenetically related to the cellular a kinase anchoring protein 7 (akap7) and sh ... | 2015 | 25878106 |
| evaluation of molecular- and culture-dependent mst markers to detect fecal contamination and indicate viral presence in good quality groundwater. | microbial contamination of groundwater represents a significant health risk to resource users. culture-dependent bacteroides phage and molecular-dependent bacteroidales 16s rrna assays are employed in microbial source tracking (mst) studies globally, however little is known regarding how these important groups relate to each other in the environment and which is more suitable to indicate the presence of waterborne fecal pollution and human enteric viruses. this study addresses this knowledge gap ... | 2015 | 25871525 |
| a prospective hospital-based surveillance to estimate rotavirus disease burden in bhutanese children under 5 years of age. | as part of efforts to develop an informed policy for rotavirus vaccination, this prospective study was conducted to estimate the burden of rotavirus diarrhea among children less than 5 years old attended to the department of pediatrics, jigme dorji wangchuk national referral hospital (jdwnrh), thimphu, bhutan. the duration of the study was three years, extending from february 2010 through december 2012. we estimated the frequency of hospitalization in the pediatric ward and dehydration treatment ... | 2015 | 25859154 |
| intrasegmental recombination does not contribute to the long-term evolution of group a rotavirus. | rotavirus is a genetically diverse pathogen with an eleven-segmented, double-stranded rna genome. intrasegmental recombination has been proposed as a potential mechanism to generate antigenic diversity and a possible route of escape from vaccine-imposed selective pressure. here intrasegmental recombination was studied by performing a genome-wide scan across the eleven genome segments of 797 publically available rotavirus strains. sixty-two sequences, or 0.7% of sequences analyzed, have evidence ... | 2015 | 25847696 |
| use of s1 nuclease in deep sequencing for detection of double-stranded rna viruses. | metagenomic approach using next-generation dna sequencing has facilitated the detection of many pathogenic viruses from fecal samples. however, in many cases, majority of the detected sequences originate from the host genome and bacterial flora in the gut. here, to improve efficiency of the detection of double-stranded (ds) rna viruses from samples, we evaluated the applicability of s1 nuclease on deep sequencing. treating total rna with s1 nuclease resulted in 1.5-28.4- and 10.1-208.9-fold incr ... | 2015 | 25843154 |
| molecular epidemiology of enteric viruses and genotyping of rotavirus a, adenovirus and astrovirus among children under 5 years old in gabon. | this study aimed to determine the prevalence of enteric viruses causing gastroenteritis, and the circulating stains, in gabonese children under five years old who visited health centers between march 2010 and june 2011. | 2015 | 25796432 |
| large scale survey of enteric viruses in river and waste water underlines the health status of the local population. | although enteric viruses constitute a major cause of acute waterborne diseases worldwide, environmental data about occurrence and viral load of enteric viruses in water are not often available. in this study, enteric viruses (i.e., adenovirus, aichivirus, astrovirus, cosavirus, enterovirus, hepatitis a and e viruses, norovirus of genogroups i and ii, rotavirus a and salivirus) were monitored in the seine river and the origin of contamination was untangled. a total of 275 water samples were colle ... | 2015 | 25795193 |
| genomic characterization and molecular investigation of vp7 epitopes of uncommon g10p[8] group a rotavirus strains detected in italy in 2009. | rotavirus strains with the uncommon genotype g10 have been detected sporadically in cases of acute gastroenteritis in humans and are thought to be transmitted zoonotically. during 2009, 10 g10p[8] rotavirus strains were detected in the stools of children hospitalized with acute diarrhoea in several paediatric hospitals in italy. the phylogenetic analysis of the vp7 gene of the italian g10p[8] strains analysed revealed nucleotide identities ranging from 94 to 99 %. molecular characterization of t ... | 2015 | 25783473 |
| molecular characterization of group a rotavirus strains detected in children with diarrhea admitted to nigerian hospitals in 2013. | group a rotaviruses (rva) are the leading cause of acute gastroenteritis (age) in children worldwide and cause up to 455,000 deaths annually, mostly in developing countries. during 2013, 66 rvas from children with age admitted to four nigerian hospitals were investigated. the g3p[6], g1p[8] and g2p[4] genotypes predominated. the vp7 and/or vp4 genes of 18 g3p[6]/[8]/[4], six g2p[4], three g12p[8]/[4], and two g1p[8] rva strains were sequenced. the g3p[6] strains belonged to lineage g3-iii and we ... | 2015 | 25772575 |
| complete genomic sequence for an avian group g rotavirus from south africa. | we report the first complete sequence for an avian group g rotavirus (rvg) genome from africa, which is the third publically available rvg genome. these rvg genomes are highly diverse, especially in their vp4, vp7, nsp4, and nsp3 segments, indicating that rvg diversity is comparable to that of rotavirus a. | 2015 | 25767240 |
| crystal structure of the c-terminal 2',5'-phosphodiesterase domain of group a rotavirus protein vp3. | in response to viral infections, the mammalian innate immune system induces the production of the second messenger 2'-5' oligoadenylate (2-5a) to activate latent ribonuclease l (rnase l) that restricts viral replication and promotes apoptosis. a subset of rotaviruses and coronaviruses encode 2',5'-phosphodiesterase enzymes that hydrolyze 2-5a, thereby inhibiting rnase l activation. we report the crystal structure of the 2',5'-phosphodiesterase domain of group a rotavirus protein vp3 at 1.39 å re ... | 2015 | 25758703 |
| unexpected spreading of g12p[8] rotavirus strains among young children in a small area of central italy. | rotavirus gastroenteritis is associated mainly with the five genotypes g1,3,4,9p[8] and g2p[4] that are common worldwide, but emerging strains including g6, g8, and g12 are also reported sporadically. g12p[8] rotavirus was observed unexpectedly to spread in a limited area of italy during the rotavirus surveillance season 2012-2013. all strains were genotyped for vp7 and vp4 and subjected to phylogenetic analysis. amino acid sequences of antigenic regions were compared with vaccine and field stra ... | 2015 | 25758365 |
| human rotavirus group a serotypes causing gastroenteritis in children less than 5 years and hiv-infected adults in viwandani slum, nairobi. | rotavirus remains a leading cause of acute gastroenteritis in children worldwide with an estimated 2000 deaths each day in developing countries. due to hiv/aids scourge in kenya, it is possible that rotavirus-related gastroenteritis has been aggravated in adults. the global alliance for immunizations has ranked rotavirus infection a priority for vaccine, and, to ensure its success, there is a need to document the local strain(s) circulating in different regions. | 2015 | 25733783 |
| h2 genotypes of g4p[6], g5p[7], and g9[23] porcine rotaviruses show super-short rna electropherotypes. | during group a rotavirus (rva) surveillance of pig farms in japan, we detected three rva strains (g4p[6], g5p[7], and g9p[23] genotypes), which showed super-short rna patterns by polyacrylamide gel electrophoresis, in samples from a healthy eight-day-old pig and two pigs of seven and eight days old with diarrhea from three farms. reverse transcription pcr and sequencing revealed that the full-length nsp5 gene of these strains contained 952 or 945 nucleotides, which is consistent with their super ... | 2015 | 25724331 |
| microbial aetiology of acute diarrhoea in children under five years of age in khartoum, sudan. | diarrhoea is one of leading causes of morbidity and mortality worldwide. recent estimations suggested the number of deaths is close to 2.5 million. this study examined the causative agents of diarrhoea in children under 5 years of age in suburban areas of khartoum, sudan. a total of 437 stool samples obtained from children with diarrhoea were examined by culture and pcr for bacteria, by microscopy and pcr for parasites and by immunoassay for detection of rotavirus a. of the 437 samples analysed, ... | 2015 | 25713206 |
| whole-genome sequence analysis of g3 and g14 equine group a rotaviruses isolated in the late 1990s and 2009-2010. | equine group a rotavirus (rva) g3p[12] and g14p[12] strains cause gastroenteritis in foals worldwide. both of these strains have been co-circulating in japan since g14p[12] strains emerged in the late 1990s. although it is important to comprehensively understand the evolution of rva strains, whole-genome sequence data on recent equine rva strains in japan are lacking. therefore, in this study, whole-genome analysis of 23 equine rva isolates from the late 1990s and 2009-2010 and the vaccine strai ... | 2015 | 25708838 |
| changing patterns of rotavirus strains circulating in ireland: re-emergence of g2p[4] and identification of novel genotypes in ireland. | worldwide, group a rotavirus (rva) is recognized as the most common aetiological agent of acute diarrheal disease in children. one hundred and ninety seven positive faecal samples were obtained from patients between 2006 and 2008. reverse transcriptase polymerase chain reaction (rt-pcr) was used to amplify the vp7 and vp4 gene segments of these samples, and g and p typing was carried out subsequently. the most common strain type was g1p[8], and the emergent global g9-type was identified in both ... | 2015 | 25704492 |
| rotavirus strains in neglected animal species including lambs, goats and camelids. | surveillance of rotavirus infections and circulating strains in small ruminants (i.e. lambs, goats and camelids) has been a neglected research area in the past. however, recent years that have seen an intensification of surveillance in humans and livestock animals, where vaccines to reduce disease burden caused by rotavirus a (rva) are available, led to the efforts to better understand the epidemiology, ecology and evolution of rva strains in other hosts, including lambs, goats and camelids. the ... | 2014 | 25674588 |
| rotavirus associated gastroenteritis in thailand. | group a rotavirus is the leading cause of severe diarrhea in infants and young children, and in young animals of many species worldwide. rotavirus is also the major cause of deaths of children younger than 5 years of age, particularly, in developing countries in asia and africa. in thailand, the burden of rotavirus infection rate in children admitted to the hospitals with acute gastroenteritis ranged from 28.4 to 44.5 %. the seasonality of rotavirus gastroenteritis in thailand was detected all-y ... | 2014 | 25674586 |
| epidemiological and clinical studies of rotavirus-induced diarrhea in china from 1994-2013. | group a rotavirus infections cause diarrhea in infants and primarily occur in winter. these viruses are characterized by the viral structural proteins vp7 (g subtypes) and vp4 (p subtypes). current treatments employ vaccines combined with symptomatic treatments. through a review of published papers from 2003-2013, which included articles, theses, dissertations and academic conference proceedings in pubmed and cnki (china national knowledge infrastructure), we examined the epidemiological and cli ... | 2014 | 25668671 |
| prevalence of rotaviruses groups a and c in egyptian children and aquatic environment. | the objective of this study is to compare the prevalence of rotaviruses groups a and c in egyptian children and aquatic environment. from 110 stool specimens of children with acute diarrhea and using rt-pcr, 35 samples (31.8 %) were positive for human rotavirus group a and 15 samples (13.6 %) were positive for human rotavirus group c. from 96 samples collected from zenin wastewater treatment plant over a 2-year period (november 2009-october 2011) and using rt-pcr, rotavirus group a was detected ... | 2015 | 25665825 |
| molecular epidemiology of group a rotavirus among children admitted to hospital in salto, uruguay, 2011-2012: first detection of the emerging genotype g12. | group a rotavirus (rva) is the most important etiologic agent of infant acute gastroenteritis (age) worldwide. detection and molecular characterization of rva in salto department, northwestern region of uruguay, was conducted on 175 clinical samples, being 153 stool and 22 vomit samples, collected from hospitalized children with age, between 0-15 years old, from two hospitals of salto city during 2011 and 2012. rva was detected and genotyped by seminested multiplex rt-pcr in order to determine g ... | 2015 | 25650154 |
| molecular characterization of equine rotaviruses isolated in europe in 2013: implications for vaccination. | equine group a rotavirus (rvas) mainly cause disease in foals under the age of 3 months. only sporadic data are available on the circulation of rvas in equine populations in europe. in this study, 65 diarrheic samples from foals under 4 months of age were collected in belgium (n=32), germany (n=17), slovenia (n=5), sweden (n=4), hungary (n=3), italy (n=2), france (n=1) and the netherlands (n=1). forty percent of these samples (n=26) were found to be rva positive by a quantitative rt-pcr assay. t ... | 2015 | 25637313 |
| full-genome sequencing of a hungarian canine g3p[3] rotavirus a strain reveals high genetic relatedness with a historic italian human strain. | a canine rotavirus a strain was identified in the fecal specimen of a young dog during 2012 in hungary. the strain rva/dog-wt/hun/135/2012/g3p[3] shared complete genotype constellation (g3-p[3]-i3-r3-c3-m3-a15-n2-t3-e3-h6) and high genome sequence similarity (nt, 98.8 %) with a historic human strain, rva/human-tc/ita/pa260-97/1997/g3p[3]. this study provides evidence for the canine origin of the unusual nsp1 genotype, a15, and reinforces the hypothesis of direct interspecies transmission of cani ... | 2015 | 25634124 |
| rotavirus and adenovirus gastroenteritis: time series analysis. | this study investigated the effects of changes in weather conditions (monthly average temperature, monthly minimum temperature, monthly average humidity) on rotavirus and adenovirus gastroenteritis frequency and whether there was a seasonal correlation. | 2015 | 25625610 |
| genetic diversity in three bovine-like human g8p[14] and g10p[14] rotaviruses suggests independent interspecies transmission events. | the group a rotavirus (rva) p[14] genotype has been detected sporadically in humans and is thought to be acquired through zoonotic transmission. the present study describes the full-length genome analysis of two g8p[14] and one g10p[14] human rvas detected in italy. the strains possessed the typical bovine-like i2-r2-c2-m2-a3/a11-n2-t6-e2-h3 genotype constellation. all the segments of the two g8p[14] rvas were most closely related to bovine(-like) strains but were relatively distant to each othe ... | 2015 | 25614586 |
| aetiology of acute paediatric gastroenteritis in bulgaria during summer months: prevalence of viral infections. | paediatric acute gastroenteritis is a global public health problem. comprehensive laboratory investigation for viral, bacterial and parasitic agents is helpful for improving management of acute gastroenteritis in health care settings and for monitoring and controlling the spread of these infections. our study aimed to investigate the role of various pathogens in infantile diarrhoea in bulgaria outside the classical winter epidemics of rotavirus and norovirus. stool samples from 115 hospitalized ... | 2015 | 25596126 |
| multicenter evaluation of the biofire filmarray gastrointestinal panel for etiologic diagnosis of infectious gastroenteritis. | the appropriate treatment and control of infectious gastroenteritis depend on the ability to rapidly detect the wide range of etiologic agents associated with the disease. clinical laboratories currently utilize an array of different methodologies to test for bacterial, parasitic, and viral causes of gastroenteritis, a strategy that suffers from poor sensitivity, potentially long turnaround times, and complicated ordering practices and workflows. additionally, there are limited or no testing met ... | 2015 | 25588652 |
| diversity of rotavirus strain circulated in gwangju, republic of korea. | the introduction of new rotavirus vaccines into the public sphere makes it necessary to maintain constant surveillance and to heighten public awareness of the appearance of new rotavirus strains. we describe the molecular epidemiology of circulating rotavirus strains after vaccine introduction. | 2014 | 25562046 |
| rapid and simultaneous detection of three major diarrhea-causing viruses by multiplex real-time nucleic acid sequence-based amplification. | rotaviruses, noroviruses and astroviruses are the major viral pathogens leading to diarrhea worldwide. epidemiological investigations of outbreaks associated with these viruses have been impeded by the lack of methods for quick diagnosis and detection. in the current study, a multiplex real-time nucleic acid sequence-based amplification (rt-nasba) system was developed for the simultaneous detection of rotavirus a/norovirus genogroup ii/astrovirus. the specificity and sensitivity of the assay wer ... | 2015 | 25559674 |
| detection and genetic characterization of porcine group a rotaviruses in asymptomatic pigs in smallholder farms in east africa: predominance of p[8] genotype resembling human strains. | viral enteritis is a serious problem accounting for deaths in neonatal animals and humans worldwide. the absence of surveillance programs and diagnostic laboratory facilities have resulted in a lack of data on rotavirus associated diarrheas in pigs in east africa. here we describe the incidence of group a rotavirus (rva) infections in asymptomatic young pigs in east africa. of the 446 samples examined, 26.2% (117/446) were positive for rva. more nursing piglets (78.7%) shed rva than weaned (32.9 ... | 2015 | 25541378 |
| rotavirus infection in children and adults with acute gastroenteritis in thailand. | and young children, but rotavirus gastroenteritis in adults is uncommon. in this study, 260 stool samples collected in thailand from january 2006 to february 2007 from patients, of all ages with acute gastroenteritis, were tested for group a rotavirus and compared with rotavirus infections in children and adults. rota- virus was detected in 42% of the patients' samples, but children (< 18 years old) have a significantly higher prevalence (57%) of rotavirus infection than adults (≥ 18 years old) ... | 2014 | 25507599 |
| full genome sequence of a reassortant human g9p[4] rotavirus strain. | this is a report of the complete genomic sequence of a reassortant rotavirus group a g9-p[4]-i2-r2-c2-m2-a2-n2-t2-e6-h2 strain designated rva/human-wt/usa/ lb1562/2010/g9p[4]. | 2014 | 25502675 |
| relationship between viral detection and turbidity in a watershed contaminated with group a rotavirus. | enteric viruses are present in aquatic environments due to contamination by raw sewage, even in the absence of fecal coliforms, which are considered to be significant indicators when it comes to microbial water quality assessment. this study investigated the presence of group a rotavirus (rva) in surface water from a river basin in minas gerais, brazil, assessing the influence of the urbanization, the rainfall, and the microbiological and physico-chemical parameters regarding water quality. fort ... | 2015 | 25471713 |
| full genome characterization of human rotavirus a strains isolated in cameroon, 2010-2011: diverse combinations of the g and p genes and lack of reassortment of the backbone genes. | over the past few years whole genome sequencing of rotaviruses has become a routine laboratory method in many strain surveillance studies. to study the molecular evolutionary pattern of representative cameroonian rotavirus a (rva) strains, the semiconductor sequencing approach was used following random amplification of genomic rna. in total, 31 rva strains collected during 2010-2011 in three cameroonian study sites located 120 to 1240 km from each other were sequenced and analyzed. sequence anal ... | 2014 | 25460824 |
| whole genomic analysis of porcine g10p[5] rotavirus strain p343 provides evidence for bovine-to-porcine interspecies transmission. | porcine group a rotavirus (rva) strain p343 (rva/pig-tc/tha/p343/1991/g10p[5]) was suggested to have vp7 and vp4 genes of bovine origin. in order to obtain precise information on the exact origin and evolution of this unusual porcine strain, the remaining nine genes (vp6, vp1-3, and nsp1-5) of strain p343 were sequenced and analyzed in the present study. on whole genomic analysis, strain p343 was found to have a bovine rva-like genotype constellation (g10-p[5]-i2-r2-c2-m2-a3-n2-t6-e2-h3) differe ... | 2014 | 25457370 |
| an outbreak of acute gastroenteritis associated with group a rotavirus in long-term care facility in slovenia. | in april 2013, an outbreak of acute gastroenteritis was reported in a residential long-term care facility (ltcf) in ljubljana. we carried out an outbreak investigation to describe the outbreak, identify factors associated with contracting infection and to recommend control measures. | 2015 | 25447968 |
| rotavirus infection in children and adults with acute gastroenteritis in thailand. | and young children, but rotavirus gastroenteritis in adults is uncommon. in this study, 260 stool samples collected in thailand from january 2006 to february 2007 from patients, of all ages with acute gastroenteritis, were tested for group a rotavirus and compared with rotavirus infections in children and adults. rota- virus was detected in 42% of the patients' samples, but children (< 18 years old) have a significantly higher prevalence (57%) of rotavirus infection than adults (≥ 18 years old) ... | 2014 | 25427349 |
| molecular epidemiology of rotavirus in cats in the united kingdom. | rotaviruses are leading causes of gastroenteritis in the young of many species. molecular epidemiological studies in children suggest that interspecies transmission contributes to rotavirus strain diversity in people. however, population-based studies of rotaviruses in animals are few. we investigated the prevalence, risk factors for infection, and genetic diversity of rotavirus a in a cross-sectional survey of cats housed within 25 rescue catteries across the united kingdom. morning litter tray ... | 2015 | 25411173 |
| norovirus and rotavirus survival in urine collected from a public ecological sanitation system in ouagadougou, burkina faso. | urine from urine-diversion toilets (udts) is routinely used as fertilizer for urban agriculture in ouagadougou, burkina faso. because urine from udts can be accidentally spoiled by feces, we determined whether virulent enteric viruses could persist in urine that is used for agricultural purposes and pose a threat to human health. urine samples (n = 60) were first collected from 42 udts during the months of january and february 2012 in ouagadougou and screened negative for the presence of norovir ... | 2015 | 25408534 |
| phylogenetic analysis of a g6p[5] bovine rotavirus strain isolated in a neonatal diarrhea outbreak in a beef cattle herd vaccinated with g6p[1] and g10p[11] genotypes. | the aim of this study was to perform the molecular characterization of the eleven genes of a g6p[5] bovine group a rotavirus (rva) strain detected in a diarrhea outbreak from a vaccinated beef cattle herd. the outbreak affected 80 % of calves between 15-30 days old. rva was identified by rt-pcr in 12 (70.6 %) out of 17 diarrheic fecal samples evaluated. the rotavirus wild-type strain had the genotype constellation g6(iv)-p[5](ix)-i2c-r2-c2-m2-a3-n2-t6-e2e-h3a. this study confirms the importance ... | 2015 | 25377636 |
| acute diarrhea in adults consulting a general practitioner in france during winter: incidence, clinical characteristics, management and risk factors. | data describing the epidemiology and management of viral acute diarrhea (ad) in adults are scant. the objective of this study was to identify the incidence, clinical characteristics, management and risk factors of winter viral ad in adults. | 2014 | 25358721 |
| dna microarray for detection of gastrointestinal viruses. | gastroenteritis is a clinical illness of humans and other animals that is characterized by vomiting and diarrhea and caused by a variety of pathogens, including viruses. an increasing number of viral species have been associated with gastroenteritis or have been found in stool samples as new molecular tools have been developed. in this work, a dna microarray capable in theory of parallel detection of more than 100 viral species was developed and tested. initial validation was done with 10 differ ... | 2014 | 25355758 |
| whole genotype constellation of prototype feline rotavirus strains frv-1 and frv64 and their phylogenetic relationships with feline-like human rotavirus strains. | feline rotaviruses, members of the species rotavirus a, are an infrequent source of zoonotic infections, and were previously shown by rna-rna hybridization assays to possess two distinct genomic rna constellations, represented by strains frv-1 and frv64. due to the lack of whole genome sequence information for frv-1, human rotavirus strain au-1 has been used as a surrogate for the genotype constellation of feline rotaviruses. the aim of this study was to determine the whole genome sequence of fr ... | 2015 | 25351516 |
| isothermal cross-priming amplification implementation study. | cross-priming amplification (cpa) was evaluated for the early detection of norovirus (nv), rotavirus a (rv-a), enteric adenovirus (eadv) and astrovirus (astv). the analytical sensitivity of the cpa assay was 10(3) copies ml(-1) for nv, rv-a and astv detection and 10(4) copies ml(-1) for eadv detection. for each of the four pathogens, the positive detection rate by cpa was similar to real-time pcr methods and higher than the rate observed in an elisa. the detection coincidence rates of cpa and rt ... | 2015 | 25346035 |
| detection of rare g3p[19] group a rotavirus in human patient, italy. | infection with a rare g3p[19] rotavirus a strain was identified in an immunosuppressed patient in italy. the strain showed a p[19] viral protein 4 gene and a complete au-1-like genomic constellation. phylogenetic analyses showed high nucleotide identity between this strain and g3p[19] rotavirus a strains from asia, indicating possible reassortment events. | 2014 | 25340712 |
| analysis of rotavirus genotypes in korea during 2013: an increase in the g2p[4] genotype after the introduction of rotavirus vaccines. | group a rotavirus is the leading cause of acute gastroenteritis in children worldwide. we investigated g and p genotypes of group a rotavirus strains isolated from patients during 2013 and investigated which genotypes were identified from vaccinated patients. | 2014 | 25312273 |
| detection of avian-like rotavirus a vp4 from a calf in japan. | a total of 568 normal feces from calves on a beef farm in fukui prefecture, japan, in 2011-2012 were examined by rt-semi-nested pcr for rotavirus a (rva) vp4 genes. through partial sequencing and blast analyses of 84 vp4-positive specimens, we identified an avian-like rva strain, n2342, which shares highest nucleotide identity (80.0%) with known avian-like bovine strain 993/83, in one specimen. phylogenetic analysis also revealed a close genetic relationship between n2342 and avian rvas, suggest ... | 2015 | 25311984 |
| a bovine g8p[1] group a rotavirus isolated from an asymptomatically infected dog. | group a rotaviruses (rvas) are enteric pathogens with well-documented zoonotic transmissions to humans. the segmented genome of the virus enables reassortment events which might alter host susceptibility and/or disease course. genetic analysis of rotavirus in dogs has so far only revealed rvas with the vp7 and vp4 genome constellation g3p[3]. rva g3p[3] have also been found in cats, humans, monkeys and bats. in the present study, we described an unusual rva of genotype g8p[1] which was isolated ... | 2015 | 25304653 |
| [evaluation of real-time multiplex pcr effectiveness for group a rotavirus genotyping]. | evaluate resolution and diagnostic significance of real-time multiplex pcr (mp rt-pcr) as a platform for group a rotavirus g/p genotyping test-systems. | 2014 | 25286527 |
| classification and characterization of a laboratory chicken rotavirus strain carrying g7p[35] neutralization antigens on the genotype 4 backbone gene configuration. | the laboratory rotavirus strain, brs/115, has been used for more than two decades to monitor rotaviruses in specific pathogen free flocks of laying hens. however, the virus strain has not been characterized in detail. therefore we aimed at the description of molecular features of brs115 by using random primed reverse transcription-pcr of the genomic rna followed by massive parallel sequencing using the semiconductor sequencing technology. over 64,000 trimmed reads mapped to reference sequences o ... | 2014 | 25284347 |
| [evolutionary relationships of g3 garv isolated from pigs and humans in lulong county, hebei province, china]. | this study aimed to amplify major genome segments (vp7, vp4, vp6, vp2 and nsp2-5) of porcine g3 group a rotavirus (garv) llz212 isolated in our laboratory, determine their genotypes, and explore the evolutionary relationships between g3 garv strains isolated from humans and pigs in lulong county, hebei province, china. major genome segments of seven garv strains were amplified by reverse transcription-polymerase chain reaction (rt-pcr) and the segments were sequenced. the genome segments of seve ... | 2014 | 25272597 |
| prevalence and genomic characterization of g2p[4] group a rotavirus strains during monovalent vaccine introduction in brazil. | this study aims to: estimate the prevalence of g2p[4] rotaviruses in brazil between 2001-2011 from patients with acute gastroenteritis; perform phylogenetic analyses of g2p[4] brazilian strains (from vaccinated and non-vaccinated children) based on vp7 and vp8(∗) encoding genes and analyze the antigenic regions of these proteins comparing with rv1; and assess the full genetic background of eleven selected brazilian strains. the g2p[4] detection rate among rva positive samples was 0/157 in 2001, ... | 2014 | 25239525 |
| prevalence of endemic enteropathogens of calves in new zealand dairy farms. | to conduct a country-wide prevalence study of bovine group a rotavirus, coronavirus, cryptosporidium parvum, salmonella spp. and enterotoxigenic k99(+) escherichia coli (k99) in calves on new zealand dairy farms. | 2015 | 25237728 |
| a possible packaging signal in the rotavirus genome. | group a rotavirus (rva), an etiological agent of gastroenteritis in young mammals and birds, possesses a genome of 11 double-stranded rna segments. although it is believed that the rva virion contains one copy of each genomic segment and that the positive-strand rna (+rna) is incorporated into the core shell, the packaging mechanisms of rva are not well understood. here, packaging signals of rva were searched for by analyzing genomic sequences of mammalian and avian rva, which are considered to ... | 2014 | 25224974 |
| noroviruses associated with outbreaks of acute gastroenteritis in the state of rio grande do sul, brazil, 2004-2011. | acute gastroenteritis norovirus (nov) in a country of continental dimensions like brazil has resulted in under-reporting of the number of outbreaks, as well as the genotypes associated. | 2014 | 25223919 |
| rotavirus genotypes in belarus, 2008-2012. | this study describes group a rotavirus (rva) genotype prevalence in belarus from 2008 to 2012. in 2008, data from 3 sites in belarus (brest, mogilev, minsk) indicated that g4p[8] was the predominant genotype. data from minsk (2008-2012) showed that g4p[8] was the predominant rva genotype in all years except in 2011 when g3p[8] was most frequently detected. other rva genotypes common in europe (g1p[8], g2p[4]) were detected each year of the study. this study reveals the dominance of genotype g4p[ ... | 2014 | 25218086 |
| rapid detection and high occurrence of porcine rotavirus a, b, and c by rt-qpcr in diagnostic samples. | rotaviruses are important cause of diarrhea in animals, including humans. currently, rotavirus species a, b, c, e, and h (rva-rvc, rve, and rvh) have been identified in pigs. traditionally, rva has been considered the primary cause of diarrhea in pigs, and rvb and rvc had been described sporadically in pigs until recently. qualitative porcine rva, rvb, and rvc rt-pcr (rt-qpcr) assays were designed and 7508 porcine diarrheic samples, submitted to university of minnesota, were tested to estimate t ... | 2014 | 25194889 |
| molecular surveillance of rotavirus infection in bangui, central african republic, october 2011-september 2013. | the world health organization (who) recommends the introduction of rotavirus vaccine in the immunization program of all countries. in the central african republic (car), sentinel surveillance for rotavirus gastroenteritis was established in 2011 by the ministry of health, with the support of the surveillance en afrique centrale project (survac). the purpose of this study was to assess the burden of rotavirus gastroenteritis and to identify rotavirus strains circulating in car before the introduc ... | 2014 | 25193563 |
| prevalence of rotavirus (garv) and coronavirus (bcov) associated with neonatal diarrhea in calves in western algeria. | to study the prevalence of bovine group a rotavirus (garv) and bovine coronavirus (bcov) in diarrheic feces from calves and the sensitive's parameters such as age group and sex. | 2014 | 25183104 |
| genomic characterization of a rotavirus g8p[1] detected in a child with diarrhea reveal direct animal-to-human transmission. | group a rotavirus is a major cause of severe gastroenteritis in children and young animals. during a retrospective analysis of samples collected from paraguayan children under 5 years old with diarrhea, and previously negative for rotavirus and norovirus, we detected the presence of bovine rotavirus sequences by viral metagenomics. nucleic acid was extracted direct from stool sample and determined to be g8p[1]. the genomic analyzes revealed that the strain presents an artiodactyl-like genome (g8 ... | 2014 | 25169054 |
| genetic diversity of porcine group a rotavirus strains in the uk. | rotavirus is endemic in pig farms where it causes a loss in production. this study is the first to characterise porcine rotavirus circulating in uk pigs. samples from diarrheic pigs with rotavirus enteritis obtained between 2010 and 2012 were genotyped in order to determine the diversity of group a rotavirus (garv) in uk pigs. a wide range of rotavirus genotypes were identified in uk pigs: six g types (vp7); g2, g3, g4, g5, g9 and g11 and six p types (vp4); p[6], p[7], p[8], p[13], p[23], and p[ ... | 2014 | 25123085 |
| metagenomic identification of novel enteric viruses in urban wild rats and genome characterization of a group a rotavirus. | rats are known as reservoirs and vectors for several zoonotic pathogens. however, information on the viruses shed by urban wild rats that could pose a zoonotic risk to human health is scare. here, intestinal contents from 20 wild norway rats (rattus norvegicus) collected in the city of berlin, germany, were subjected to metagenomic analysis of viral nucleic acids. the determined faecal viromes of rats consisted of a variety of known and unknown viruses, and were highly variable among the individ ... | 2014 | 25121550 |
| whole genomic analysis of human g12p[6] and g12p[8] rotavirus strains that have emerged in kenya: identification of porcine-like nsp4 genes. | g12 rotaviruses are globally emerging rotavirus strains causing severe childhood diarrhea. however, the whole genomes of only a few g12 strains have been fully sequenced and analyzed, of which only one g12p[4] and one g12p[6] are from africa. in this study, we sequenced and characterized the complete genomes of three g12 strains (rva/human-tc/ken/kdh633/2010/g12p[6], rva/human-tc/ken/kdh651/2010/g12p[8], and rva/human-tc/ken/kdh684/2010/g12p[6]) identified in three stool specimens from children ... | 2014 | 25111611 |
| molecular epidemiology of rotavirus in children and animals and characterization of an unusual g10p[15] strain associated with bovine diarrhea in south india. | rotaviruses are enteric pathogens causing acute, watery, dehydrating diarrhea in various host species, including birds and mammals. this study collected data on the disease burden and strain prevalence of group a rotavirus in animals and humans in vellore and investigated interspecies transmission by comparison of circulating genotypes. stool samples from children aged less than 5 years, admitted to the hospital between january 2003 and may 2006 for diarrhea and diarrheal samples from animals th ... | 2014 | 25091687 |
| characterisation of rotavirus strains identified in adolescents and adults with acute gastroenteritis highlights circulation of non-typeable strains: 2008-2012. | group-a rotavirus (rv) is the main causative agent of acute gastroenteritis in children < 5 years of age. its role as a pathogen in adults needs to be monitored. the aim of this study was to characterise the group-a rv strains that cause infections of acute gastroenteritis in adolescents and adults and determine the temporal variations in the circulating strains during 2008-2012 in continuation of an earlier study conducted in 2004-2007, in pune, india. | 2014 | 25091684 |
| group a rotavirus gastroenteritis in older children and adults at a hospital in southern india. | there is limited data on the spectrum and prevalence of rotavirus genotypes in older children and adults in asia. this pilot study conducted between november 2012 and april 2013 tested for rotavirus in older children (>12 years of age), and adults with gastroenteritis from southern india. stool samples from patients who were hospitalized or attended out-patient units with diarrhea were screened for rotavirus using premier™ rotaclone(®). confirmatory testing was by another antigen detection sandw ... | 2014 | 25091677 |
| acute gastroenteritis and enteric viruses in hospitalised children in southern brazil: aetiology, seasonality and clinical outcomes. | viral acute gastroenteritis (ag) is a significant cause of hospitalisation in children younger than five years. group a rotavirus (rva) is responsible for 30% of these cases. following the introduction of rva immunisation in brazil in 2006, a decreased circulation of this virus has been observed. however, ag remains an important cause of hospitalisation of paediatric patients and only limited data are available regarding the role of other enteric viruses in these cases. we conducted a prospectiv ... | 2014 | 25075782 |
| simultaneous concentration of bovine viruses and agricultural zoonotic bacteria from water using sodocalcic glass wool filters. | infiltration and runoff from manured agricultural fields can result in livestock pathogens reaching groundwater and surface waters. here, we measured the effectiveness of glass wool filters to simultaneously concentrate enteric viruses and bacteria of bovine origin from water. the recovery efficiencies were determined for bovine viral diarrhea virus types 1 and 2, bovine rotavirus group a, bovine coronavirus, poliovirus sabin iii, toxigenic escherichia coli ,and campylobacter jejuni seeded into ... | 2014 | 25059211 |
| human group a rotavirus infections in children in denmark: detection of reassortant g9 strains and zoonotic p[14] strains. | one of the leading causes of severe childhood gastroenteritis are group a rotaviruses, and they have been found to be associated with ∼40% of the annual gastroenteritis-associated hospitalizations in young danish children <5years of age (fischer et al., 2011). in this study, we investigated the diversity of rotavirus strains circulating among young children <5years of age, presenting with gastroenteritis disease either at the general practitioner or in the hospital, during the period 2009-2013. ... | 2014 | 25038295 |
| host-pathogen co-evolution and glycan interactions. | noroviruses and rotavirus a bind to polymorphic glycans of the histo-blood group type (hbgas). norovirus strains that bind to hbgas can collectively infect all humans but each strain only infects a subgroup of the population, suggesting a past co-evolution that led to a trade-off where the human population is partly protected whilst the virus circulation is maintained. we termed 'herd innate protection' the host species partial protection provided by the hbgas polymorphism. given its recent emer ... | 2014 | 25000207 |
| gastroenteric virus dissemination and influence of rainfall events in urban beaches in brazil. | this study was conducted to assess rotavirus a (rv-a), genogroup ii (gii) norovirus (nov), and human adenovirus (hadv) dissemination in recreational water in an urban beach located in the city of rio de janeiro and their persistence during rainfall events. | 2014 | 24980661 |
| rotavirus surveillance in kisangani, the democratic republic of the congo, reveals a high number of unusual genotypes and gene segments of animal origin in non-vaccinated symptomatic children. | group a rotavirus (rva) infections form a major public health problem, especially in low-income countries like the democratic republic of the congo (cod). however, limited data on rva diversity is available from sub-saharan africa in general and the cod in particular. therefore, the first aim of this study was to determine the genetic diversity of 99 rvas detected during 2007-2010 in kisangani, cod. the predominant g-type was g1 (39%) and the most predominant p-type was p[6] (53%). a total of ei ... | 2014 | 24968018 |
| complete genome analysis of a rare group a rotavirus, g11p[25], isolated from a child in mumbai, india, reveals interspecies transmission and reassortment with human rotavirus strains. | hospital-based rotavirus surveillance was carried out in mumbai during 2005-2009. an isolate (b08299) with a rare genotype combination (g11p[25]) was detected. the present study was undertaken to characterize the complete genome of the isolate. b08299 exhibited a g11-p[25]-i12-r1-c1-m1-a1-n1-t1-e1-h1 genotype constellation. phylogenetic analysis of the 11 gene segments of b08299 revealed that the vp2 and nsp5 genes of b08299 had a human origin, while the vp6 gene represented an i12 genotype of o ... | 2014 | 24951672 |
| [whole genome analysis of human group a rotavirus g9p[8] strains in hebei lulong region, 2009-2011]. | abstract:this study aims to investigate the genetic characteristics of group a rotavirus (garv) g9p[8] strains from infantile diarrhea samples in hebei lulong region from 2009 to 2011. we randomly selected five garv g9p[8] strains in hebei lulong region from 2009 to 2011, amplified the 11 gene fragments of garvs by rt-pcr, and analyz their full-genome sequences by homology and phylogenetic analysis with dnastar and mega. the nucleotide homology between strains ll11131077 and ll11131083 in 2011 w ... | 2014 | 24923163 |
| predicted structure and domain organization of rotavirus capping enzyme and innate immune antagonist vp3. | rotaviruses and orbiviruses are nonturreted reoviridae members. the rotavirus vp3 protein is a multifunctional capping enzyme and antagonist of the interferon-induced cellular oligoadenylate synthetase-rnase l pathway. despite mediating important processes, vp3 is the sole protein component of the rotavirus virion whose structure remains unknown. in the current study, we used sequence alignment and homology modeling to identify features common to nonturreted reoviridae capping enzymes and to pre ... | 2014 | 24899176 |
| surveillance of group a rotavirus in buenos aires 2008-2011, long lasting circulation of g2p[4] strains possibly linked to massive monovalent vaccination in the region. | group a rotaviruses (rva) are the most frequent single etiological agents of severe diarrhea in infants. since 2006 rva vaccines have been introduced in national schedules of middle and high income countries with substantial declines in rotavirus associated disease burden. however, surveillance must be maintained to, eventually, detect emerging types or variants selected by the new pressure imposed by vaccination. | 2014 | 24875137 |
| comparison of pathogenicities and nucleotide changes between porcine and bovine reassortant rotavirus strains possessing the same genotype constellation in piglets and calves. | although reassortment is one of the most important characteristics of group a rotavirus (rva) evolution, the host range restriction and/or virulence of reassortant rvas remain largely unknown. the porcine 174-1 strain isolated from a diarrheic piglet was identified as a reassortant strain, harboring the same genotype constellation as the previously characterized bovine strain kj56-1. owing to its same genotype constellation, the pathogenicity of the porcine strain 174-1 in piglets and calves was ... | 2014 | 24861840 |
| gastroenteritis outbreaks caused by a ds-1-like g1p[8] rotavirus strain, japan, 2012-2013. | rotavirus a (rva) genotype g1p[8], a hallmark of the wa-like strain, typically contains only genotype 1 genes. however, an unusual rva g1p[8] with genotype 2 genes was recently detected in japan. we determined the complete genomic constellation of this rva. our findings suggest that mixed rvas may be more competitive than once thought. | 2014 | 24856174 |
| alterations in oxidant/antioxidant balance, high-mobility group box 1 protein and acute phase response in cross-bred suckling piglets suffering from rotaviral enteritis. | rotaviral enteritis has emerged as a major cause of morbidity and mortality in piglets during their post-natal life. the present study was carried out to examine high-mobility group box 1 (hmgb1) protein, acute phase response and oxidative stress indices in the serum of suckling piglets suffering from enteritis with or without association of porcine group a rotavirus infection. the present investigation utilized 23 clinical cases with signs of acute enteritis and 12 more healthy piglets of a sim ... | 2014 | 24848720 |
| relative abundance and treatment reduction of viruses during wastewater treatment processes--identification of potential viral indicators. | waterborne pathogenic viruses discharged from wastewater treatment plants (wwtps) pose potential public health risks. in the present study, we investigated the relative abundance, occurrence, and reduction of eleven different viruses at two wwtps in southern arizona over a 12-month period, from august 2011 to july 2012. influent and effluent samples from the two wwtps were collected monthly. viruses were concentrated using an electronegative filter method and quantified using taqman-based quanti ... | 2014 | 24836386 |
| [investigation of seven different rna viruses associated with gastroenteritis in children under five years old]. | viruses are the most frequently detected etiologic agents of gastroenteritis seen in small children. in addition to classical gastroenteritis viruses namely rotavirus, norovirus, adenovirus type 40/41, astrovirus and sapovirus, some novel picornaviruses (aichi virus, parechovirus, enterovirus) that have been identified in parallel to the developments in molecular diagnostic methods, thought to be associated with diarrhea in humans. however, the data are not enough to prove their actual roles in ... | 2014 | 24819261 |
| molecular detection of human noroviruses in influent and effluent samples from two biological sewage treatment plants in the region of monastir, tunisia. | noroviruses (novs) are responsible for numerous cases of waterborne and foodborne gastroenteritis every year. they are released in the sewage and their detection in this environment can reflect the epidemiology of the viral strains circulating in the community. a three-year (2007-2010) survey was conducted in order to evaluate the presence of human novs using rt-pcr in 518 sewage samples collected at the entrance and exit of two biological sewage treatment plants located in monastir region, tuni ... | 2014 | 24818568 |
| assessment of water quality in a border region between the atlantic forest and an urbanised area in rio de janeiro, brazil. | the preservation of water resources is one of the goals of the designation of parks that act as natural reservoirs. in order to assess the impact of the presence of humans in an environmental preservation area bordering urban areas, the presence of four pathogenic enteric viruses [group a rotavirus (rv-a), norovirus (nov), human adenoviruses (hadv), and hepatitis a virus (hav)], as well as the physico-chemical parameters, and escherichia coli levels were assessed in riverine water samples. from ... | 2014 | 24818567 |
| rotavirus inhibits ifn-induced stat nuclear translocation by a mechanism that acts after stat binding to importin-α. | the importance of innate immunity to rotaviruses is exemplified by the range of strategies evolved by rotaviruses to interfere with the ifn response. we showed previously that rotaviruses block gene expression induced by type i and ii ifns, through a mechanism allowing activation of signal transducer and activator of transcription (stat) 1 and stat2 but preventing their nuclear accumulation. this normally occurs through activated stat1/2 dimerization, enabling an interaction with importin α5 tha ... | 2014 | 24814927 |
| screening of viral pathogens from pediatric ileal tissue samples after vaccination. | in 2010, researchers reported that the two us-licensed rotavirus vaccines contained dna or dna fragments from porcine circovirus (pcv). although pcv, a common virus among pigs, is not thought to cause illness in humans, these findings raised several safety concerns. in this study, we sought to determine whether viruses, including pcv, could be detected in ileal tissue samples of children vaccinated with one of the two rotavirus vaccines. a broad spectrum, novel dna detection technology, the lawr ... | 2014 | 24778651 |