Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| heme oxygenase-1 and carbon monoxide promote burkholderia pseudomallei infection. | the environmental bacterium and potential biothreat agent burkholderia pseudomallei causes melioidosis, an often fatal infectious disease. increased serum bilirubin has been shown to be a negative predictive factor in melioidosis patients. we therefore investigated the role of heme oxygenase-1 (ho-1), which catalyzes the degradation of heme into the bilirubin precursor biliverdin, ferrous iron, and co during b. pseudomallei infection. we found that infection of murine macrophages induces ho-1 ex ... | 2016 | 27316684 |
| phylogenomic analysis reveals an asian origin for african burkholderia pseudomallei and further supports melioidosis endemicity in africa. | burkholderia pseudomallei, an environmental bacterium that causes the deadly disease melioidosis, is endemic in northern australia and southeast asia. an increasing number of melioidosis cases are being reported in other tropical regions, including africa and the indian ocean islands. b. pseudomallei first emerged in australia, with subsequent rare dissemination event(s) to southeast asia; however, its dispersal to other regions is not yet well understood. we used large-scale comparative genomic ... | 2016 | 27303718 |
| glanders and the risk for its introduction through the international movement of horses. | glanders is the contagious zoonotic disease caused by infection with burkholderia mallei. it affects primarily horses, donkeys and mules. the disease was eradicated from large areas of the western world in the early 20th century, but, over the last 10-20 years, has emerged and re-emerged in areas in which it was previously unknown or had been eradicated. although glanders was previously thought to manifest in only acute or chronic presentations, it now appears that b. mallei can produce latent i ... | 2016 | 27288893 |
| detection of burkholderia pseudomallei toxin-mediated inhibition of protein synthesis using a caenorhabditis elegans ugt-29 biosensor. | toxins are believed to play a crucial role in burkholderia pseudomallei pathogenicity, however to date, only a few have been identified. the discovery of additional toxic molecules is limited by the lack of a sensitive indicator of b. pseudomallei toxicity. previously, from a whole genome transcriptome analysis of b. pseudomallei-infected caenorhabditis elegans, we noted significant overexpression of a number of worm genes encoding detoxification enzymes, indicating the host's attempt to clear b ... | 2016 | 27273550 |
| possible misidentification of species in the pseudomonas fluorescens lineage as burkholderia pseudomallei and francisella tularensis, and emended descriptions of pseudomonas brenneri,pseudomonas gessardii and pseudomonas proteolytica. | bacteria were isolated from an industrial water circuit in the netherlands. these strains were identified using api 20 ne as possible, or likely, burkholderia pseudomallei. with vitek 2 some of these strains scored 'low discrimination' for francisella tularensis, amongst others. a total of twenty-six strains were assigned to the species pseudomonas brenneri, pseudomonas gessardii or pseudomonas proteolytica. because of the possibility of misidentification of these environmental species as medica ... | 2016 | 27259517 |
| triggering receptor expressed on myeloid cells (trem)-2 impairs host defense in experimental melioidosis. | triggering receptor expressed on myeloid cells (trem) -1 and trem-2 are key regulators of the inflammatory response that are involved in the clearance of invading pathogens. melioidosis, caused by the "tier 1" biothreat agent burkholderia pseudomallei, is a common form of community-acquired sepsis in southeast-asia. trem-1 has been suggested as a biomarker for sepsis and melioidosis. we aimed to characterize the expression and function of trem-1 and trem-2 in melioidosis. | 2016 | 27253382 |
| an imported case of acute melioidosis caused by st881 burkholderia pseudomallei. | a previously healthy chinese male working in malaysia returned to china with high fever. a blood culture showed burkholderia pseudomallei strain wcbp1. this isolate was sequenced, showing type, st881, which appears to be present in malaysia. wcp1 had unusual susceptibility to aminoglycosides and habored the yersinia-like fimbrial gene cluster for virulence. the patient's condition deteriorated rapidly but he recovered after receiving meropenem and intensive care support. melioidosis is a potenti ... | 2016 | 27244959 |
| polyphosphate kinase mediates antibiotic tolerance in extraintestinal pathogenic escherichia coli pcn033. | extraintestinal pathogenic escherichia coli (expec) causes a variety of acute infections in its hosts, and multidrug-resistant strains present significant challenges to public health and animal husbandry. therefore, it is necessary to explore new drug targets to control e. coli epidemics. previous studies have reported that ppk mutants of burkholderia pseudomallei and mycobacterium tuberculosis are more susceptible than the wild types (wts) to stress. therefore, we investigated the stress respon ... | 2016 | 27242742 |
| a case of systemic melioidosis: unravelling the etiology of chronic unexplained fever with multiple presentations. | melioidosis, caused by the environmental saprophyte, burkholderia pseudomallei, is an important public health problem in southeast asia and northern australia. it is being increasingly reported from other parts, including india, china, and north and south america expanding the endemic zone of the disease. we report a case of systemic melioidosis in a 58-year-old diabetic, occupationally-unexposed male patient, who presented with chronic fever, sepsis, pneumonia, pleural effusion and subcutaneous ... | 2016 | 27238172 |
| structural insights into an oxalate-producing serine hydrolase with an unusual oxyanion hole and additional lyase activity. | in burkholderia species, the production of oxalate, an acidic molecule, is a key event for bacterial growth in the stationary phase. oxalate plays a central role in maintaining environmental ph, which counteracts inevitable population-collapsing alkaline toxicity in amino acid-based culture medium. in the phytopathogen burkholderia glumae, two enzymes are responsible for oxalate production. first, the enzyme oxalate biosynthetic component a (obca) catalyzes the formation of a tetrahedral c6-coa ... | 2016 | 27226606 |
| isotype switching: mouse igg3 constant region drives increased affinity for polysaccharide antigens. | 2016 | 27224549 | |
| maldi-tof ms contribution to diagnosis of melioidosis in a nonendemic country in three french travellers. | melioidosis is an endemic disease in southeast asia and northern australia. an increasing number of cases are being reported in nonendemic countries, making the diagnosis less obvious. we discuss the identification of burkholderia pseudomallei using matrix-assisted desorption ionization-time of flight mass spectrometry on the occasion of recent cases of imported melioidosis in french travellers. | 2016 | 27222715 |
| therapeutic administration of a monoclonal anti-il-1β antibody protects against experimental melioidosis. | melioidosis, caused by the gram-negative bacterium burkholderia pseudomallei, is a common cause of community-acquired sepsis in southeast asia and northern australia. the nlrp3 inflammasome and its downstream product interleukin-1 beta (il-1β) have been proposed to play crucial roles in melioidosis. in this study, we characterized the role of il-1β more closely and we assessed its therapeutic potential. | 2016 | 27219859 |
| thiolactomycin-based inhibitors of bacterial β-ketoacyl-acp synthases with in vivo activity. | β-ketoacyl-acp synthases (kas) are key enzymes involved in the type ii bacterial fatty acid biosynthesis (fasii) pathway and are putative targets for antibacterial discovery. several natural product kas inhibitors have previously been reported, including thiolactomycin (tlm), which is produced by nocardia spp. here we describe the synthesis and characterization of optically pure 5r-thiolactomycin (tlm) analogues that show improved whole cell activity against bacterial strains including methicill ... | 2016 | 27187871 |
| fatal melioidosis in a newborn from hainan, china. | herein, we report a case of fatal melioidosis in a newborn. the newborn died of serious melioidosis with respiratory and multiorgan failure at 16 days of age despite extensive treatment with antibiotics and methylprednisolone. burkholderia pseudomallei was isolated from the infant's blood and cerebrospinal fluid and identified as a novel sequence type (st-1341). his father had cough and fever when the newborn was born, and a localized patchy infiltration on the right upper lung was seen in chest ... | 2016 | 27162267 |
| perturbation of the two-component signal transduction system, bprrs, results in attenuated virulence and motility defects in burkholderia pseudomallei. | burkholderia pseudomallei is the causative agent of melioidosis, a severe invasive disease of humans and animals. initial screening of a b. pseudomallei signature-tagged mutagenesis library identified an attenuated mutant with a transposon insertion in a gene encoding the sensor component of an uncharacterised two-component signal transduction system (tcsts), which we designated bprrs. | 2016 | 27147217 |
| what clinical factors are associated with mortality in septicemic melioidosis? a report from an endemic area. | melioidosis, caused by burkholderia pseudomallei, has high mortality, particularly in its septicemic form. data on the factors associated with mortality from melioidosis are still limited. | 2016 | 27131004 |
| protection against experimental melioidosis with a synthetic manno-heptopyranose hexasaccharide glycoconjugate. | melioidosis is an emerging infectious disease caused by burkholderia pseudomallei and is associated with high morbidity and mortality rates in endemic areas. antibiotic treatment is protracted and not always successful; even with appropriate therapy, up to 40% of individuals presenting with melioidosis in thailand succumb to infection. in these circumstances, an effective vaccine has the potential to have a dramatic impact on both the scale and the severity of disease. currently, no vaccines are ... | 2016 | 27124182 |
| surgical treatment of pseudoaneurysm of innominate artery infected with burkholderia pseudomallei. | the management of melioidosis, caused by burkholderia pseudomallei, presenting as an infected pseudoaneurysm requires radical debridement and prolonged antibiotics because the pathogen is resistant to host immunity. an extra-anatomical bypass might be a better treatment choice than in situ graft interposition or other methods.we report on a 76-year-oldman with an infected pseudoaneurysmlocated in the innominate artery and a method of extra-anatomical bypass that has not yet been reported in the ... | 2013 | 27122691 |
| contribution of murine igg fc regions to antibody binding to the capsule of burkholderia pseudomallei. | immunoglobulin g3 (igg3) is the predominant igg subclass elicited in response to polysaccharide antigens in mice. this specific subclass has been shown to crosslink its fragment crystallizable (fc) regions following binding to multivalent polysaccharides. crosslinking leads to increased affinity through avidity, which theoretically should lead to more effective protection against bacteria and yeast displaying capsular polysaccharides on their surface. to investigate this further we have analyzed ... | 2016 | 27096636 |
| evaluation of the bruker biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry system for identification of clinical and environmental isolates of burkholderia pseudomallei. | burkholderia pseudomallei is not represented in the current version of bruker biotyper matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (maldi-tof ms) system. a total of 66 isolates of b. pseudomallei, including 30 clinical isolates collected from national taiwan university hospital (ntuh, n = 27) and peking union medical college hospital (pumch, n = 3), and 36 isolates of genetically confirmed strains, including 13 from clinical samples and 23 from environmental samp ... | 2016 | 27092108 |
| burkholderia pseudomallei capsule exacerbates respiratory melioidosis but does not afford protection against antimicrobial signaling or bacterial killing in human olfactory ensheathing cells. | melioidosis, caused by the bacterium burkholderia pseudomallei, is an often severe infection that regularly involves respiratory disease following inhalation exposure. intranasal (i.n.) inoculation of mice represents an experimental approach used to study the contributions of bacterial capsular polysaccharide i (cps i) to virulence during acute disease. we used aerosol delivery of b. pseudomallei to establish respiratory infection in mice and studied cps i in the context of innate immune respons ... | 2016 | 27091931 |
| identification of an ompw homologue in burkholderia pseudomallei, a protective vaccine antigen against melioidosis. | burkholderia pseudomallei is the causative agent of melioidosis, which is associated with a range of clinical manifestations, including sepsis and fatal pneumonia and is endemic in southeast asia and northern australia. treatment can be challenging and control of infection involves prolonged antibiotic therapy, yet there are no approved vaccines available to prevent infection. our aim was to develop and assess the potential of a prophylactic vaccine candidate targeted against melioidosis. the id ... | 2016 | 27091689 |
| identification of cd4+ t-cell epitope and investigation of hla distribution for the immunogenic proteins of burkholderia pseudomallei using in silico approaches - a key vaccine development strategy for melioidosis. | melioidosis is a serious infectious diseases affecting multi-organ system in humans with high mortality rate. the disease is caused by the bacterium, burkholderia pseudomallei and it is intrinsically resistant to many antibiotics. thus, there is an urgent need for protective vaccine against b. pseudomallei; which may reduce morbidity and mortality in endemic areas. the identification of peptides that bind to major histocompatibility complex ii class helps in understanding the nature of immune re ... | 2016 | 27086038 |
| melioidosis: where do we stand in the development of an effective vaccine? | 2016 | 27081770 | |
| seroprevalence of burkholderia pseudomallei among adults in coastal areas in southwestern india. | although melioidosis, is an important disease in many southeast asian countries and australia, there is limited data on its prevalence and disease burden in india. however, an increase in case reports of melioidosis in recent years indicates its endemicity in india. | 2016 | 27078156 |
| quorum sensing influences burkholderia thailandensis biofilm development and matrix production. | members of the genus burkholderia are known to be adept at biofilm formation, which presumably assists in the survival of these organisms in the environment and the host. biofilm formation has been linked to quorum sensing (qs) in several bacterial species. in this study, we characterized burkholderia thailandensis biofilm development under flow conditions and sought to determine whether qs contributes to this process. b. thailandensis biofilm formation exhibited an unusual pattern: the cells fo ... | 2016 | 27068594 |
| burkholderia genome mining for nonribosomal peptide synthetases reveals a great potential for novel siderophores and lipopeptides synthesis. | burkholderia is an important genus encompassing a variety of species, including pathogenic strains as well as strains that promote plant growth. we have carried out a global strategy, which combined two complementary approaches. the first one is genome guided with deep analysis of genome sequences and the second one is assay guided with experiments to support the predictions obtained in silico. this efficient screening for new secondary metabolites, performed on 48 gapless genomes of burkholderi ... | 2016 | 27060604 |
| erratum to: burkholderia pseudomallei induces il-23 production in primary human monocytes. | 2016 | 27048481 | |
| identification of the burkholderia pseudomallei bacteriophage st79 lysis gene cassette. | to identify and characterize the lysis gene cassette from the bacteriophage st79 that lyses burkholderia pseudomallei. | 2016 | 27038077 |
| melioidosis and glanders modulation of the innate immune system: barriers to current and future vaccine approaches. | burkholderia pseudomallei and burkholderia mallei are pathogenic bacteria causing fatal infections in animals and humans. both organisms are classified as tier 1 select agents owing to their highly fatal nature, potential/prior use as bioweapons, severity of disease via respiratory exposure, intrinsic resistance to antibiotics, and lack of a current vaccine. disease manifestations range from acute septicemia to chronic infection, wherein the facultative intracellular lifestyle of these organisms ... | 2016 | 27010618 |
| investigation of recurrent melioidosis in lao people's democratic republic by multilocus sequence typing. | melioidosis is an infectious disease caused by the saprophytic bacterium burkholderia pseudomallei in northeast thailand and northern australia, where the disease is highly endemic, a range of molecular tools have been used to study its epidemiology and pathogenesis. in the lao people's democratic republic (laos) where melioidosis has been recognized as endemic since 1999, no such studies have been undertaken. we used a multilocus sequence typing scheme specific for b. pseudomallei to investigat ... | 2016 | 27001759 |
| serological evidence of infection with endemic human pathogens among free-ranging old world monkeys in puerto rico. | serum specimens from free-ranging but nonnative patas monkeys (erythrocebus patas) and rhesus macaques (macaca mulatta) in southwestern puerto rico (pr) were tested for antibodies to infection with dengue viruses (denvs), west nile virus (wnv), leptospira species, and burkholderia pseudomallei by microneutralization, plaque reduction neutralization, microscopic agglutination, and indirect hemagglutination, respectively. of 23 animals (21 e. patas and two m. mulatta) tested, all had evidence of p ... | 2016 | 27001754 |
| molecular evidence of burkholderia pseudomallei genotypes based on geographical distribution. | background. central intermediary metabolism (cim) in bacteria is defined as a set of metabolic biochemical reactions within a cell, which is essential for the cell to survive in response to environmental perturbations. the genes associated with cim are commonly found in both pathogenic and non-pathogenic strains. as these genes are involved in vital metabolic processes of bacteria, we explored the efficiency of the genes in genotypic characterization of burkholderia pseudomallei isolates, compar ... | 2016 | 26998408 |
| functional genome screening to elucidate the colistin resistance mechanism. | antibiogram profile of 1590 clinical bacterial isolates based on thirteen different antimicrobial compounds showed that 1.6% of the bacterial isolates are multidrug resistant. distribution pattern based on 16s rrna sequence analysis showed that pseudomonas aeruginosa constituted the largest group (83.6%) followed by burkholderia pseudomallei sp. a191 (5.17%), staphylococcus sp. a261 (3.45%). among the various antibiotics used, colistin appeared to be the most effective against the gram negative ... | 2016 | 26988670 |
| rapid antimicrobial susceptibility testing of bacillus anthracis, yersinia pestis, and burkholderia pseudomallei by use of laser light scattering technology. | rapid methods to determine antimicrobial susceptibility would assist in the timely distribution of effective treatment or postexposure prophylaxis in the aftermath of the release of bacterial biothreat agents such as bacillus anthracis, yersinia pestis, or burkholderia pseudomallei conventional susceptibility tests require 16 to 48 h of incubation, depending on the bacterial species. we evaluated a method that is based on laser light scattering technology that measures cell density in real time. ... | 2016 | 26984973 |
| experimental persistent infection of balb/c mice with small-colony variants of burkholderia pseudomallei leads to concurrent upregulation of pd-1 on t cells and skewed th1 and th17 responses. | burkholderia pseudomallei (b. pseudomallei), the causative agent of melioidosis, is a deadly pathogen endemic across parts of tropical south east asia and northern australia. b. pseudomallei can remain latent within the intracellular compartment of the host cell over prolonged periods of time, and cause persistent disease leading to treatment difficulties. understanding the immunological mechanisms behind persistent infection can result in improved treatment strategies in clinical melioidosis. | 2016 | 26974441 |
| characteristics, clinical outcomes and factors influencing mortality of patients with melioidosis in southern thailand: a 10-year retrospective study. | to study characteristics, clinical outcomes and factors influencing mortality of patients afflicted with melioidosis. | 2016 | 26972397 |
| burkholderia pseudomallei: first case of melioidosis in portugal. | burkholderia pseudomallei is a gram-negative bacillus and the causative agent of melioidosis, a serious infection associated with high mortality rate in humans. it can be naturally found as an environmental saprophyte in soil or stagnant water, and rice paddies that predominate in regions of endemicity such as northeast thailand. b. pseudomallei is a biosafety level 3 organism due to risks of aerosolization and severe disease and is now included in formal emergency preparedness plans and guideli ... | 2016 | 26962474 |
| musculoskeletal melioidosis: an under-diagnosed entity in developing countries. | melioidosis is a saprophytic infectious disease caused by gram-negative soil-dwelling bacillus burkholderia pseudomallei. | 2016 | 26955222 |
| the association of melioidosis with climatic factors in darwin, australia: a 23-year time-series analysis. | melioidosis is an often fatal disease in humans and animals and endemic in southeast asia and northern australia. it is caused by the environmental bacterium burkholderia pseudomallei. we analysed weather and climate factors preceding new melioidosis cases in darwin and compared the time between weather event and admission to hospital for severe and average wet season rainfall. | 2016 | 26945846 |
| burkholderia pseudomallei colony morphotypes show a synchronized metabolic pattern after acute infection. | burkholderia pseudomallei is a water and soil bacterium and the causative agent of melioidosis. a characteristic feature of this bacterium is the formation of different colony morphologies which can be isolated from environmental samples as well as from clinical samples, but can also be induced in vitro. previous studies indicate that morphotypes can differ in a number of characteristics such as resistance to oxidative stress, cellular adhesion and intracellular replication. yet the metabolic fe ... | 2016 | 26943908 |
| the melioidosis agent burkholderia pseudomallei and related opportunistic pathogens detected in faecal matter of wildlife and livestock in northern australia. | the darwin region in northern australia has experienced rapid population growth in recent years, and with it, an increased incidence of melioidosis. previous studies in darwin have associated the environmental presence of burkholderia pseudomallei, the causative agent of melioidosis, with anthropogenic land usage and proximity to animals. in our study, we estimated the occurrence of b. pseudomallei and burkholderia spp. relatives in faecal matter of wildlife, livestock and domestic animals in th ... | 2016 | 26935879 |
| metabolomic profiling of plasma from melioidosis patients using uhplc-qtof ms reveals novel biomarkers for diagnosis. | to identify potential biomarkers for improving diagnosis of melioidosis, we compared plasma metabolome profiles of melioidosis patients compared to patients with other bacteremia and controls without active infection, using ultra-high-performance liquid chromatography-electrospray ionization-quadruple time-of-flight mass spectrometry. principal component analysis (pca) showed that the metabolomic profiles of melioidosis patients are distinguishable from bacteremia patients and controls. using mu ... | 2016 | 26927094 |
| immunisation with proteins expressed during chronic murine melioidosis provides enhanced protection against disease. | there is an urgent need for an effective vaccine against human disease caused by burkholderia pseudomallei, and although a wide range of candidates have been tested in mice none provide high level protection. we considered this might reflect the inability of these vaccine candidates to protect against chronic disease. using q-rt pcr we have identified 6 genes which are expressed in bacteria colonising spleens and lungs of chronically infected mice. three of the genes (bpsl1897, bpsl3369 and bpsl ... | 2016 | 26917010 |
| development of rapid enzyme-linked immunosorbent assays for detection of antibodies to burkholderia pseudomallei. | burkholderia pseudomallei, the causative agent of melioidosis, is an environmental bacillus found in northeast thailand. the mortality rate of melioidosis is ∼40%. an indirect hemagglutination assay (iha) is used as a reference serodiagnostic test; however, it has low specificity in areas where the background seropositivity of healthy people is high. to improve assay specificity and reduce the time for diagnosis, four rapid enzyme-linked immunosorbent assays (elisas) were developed using two pur ... | 2016 | 26912754 |
| role of burkholderia pseudomallei sigma n2 in amino acids utilization and in regulation of catalase e expression at the transcriptional level. | burkholderia pseudomallei is the causative agent of melioidosis. the complete genome sequences of this pathogen have been revealed, which explain some pathogenic mechanisms. in various hostile conditions, for example, during nitrogen and amino acid starvation, bacteria can utilize alternative sigma factors such as rpos and rpon to modulate genes expression for their adaptation and survival. in this study, we demonstrate that mutagenesis of rpon2, which lies on chromosome 2 of b. pseudomallei and ... | 2015 | 26904748 |
| paediatric neurological melioidosis: a rehabilitation case report. | melioidosis is a rare condition, endemic to northern australia and south-east asia, caused by an infection from the bacteria <i>burkholderia pseudomallei</i>. the largest epidemiological review to date describes 540 cases of melioidosis seen at darwin hospital, in northern australia, over a 20-year period. of these, 14 (less than 3%) presented with neurological manifestation, with three deaths. reports of paediatric cases of melioidosis are rarer. in a review of paediatric cases in northern aust ... | 2016 | 26897103 |
| central nervous system melioidosis mimics malignancy: a case report and literature review. | central nervous system (cns) melioidosis is notorious because of the difficulty in bacteria eradication and the destruction of brain structures. early manifestation of cns melioidosis mimics malignancy or stroke. we present a case of cns melioidosis that initially manifested as malignancy. | 2016 | 26882971 |
| successful treatment outcome of primary melioidosis pneumonia-a case report from bangladesh. | melioidosis is endemic in tropical australia and southeast asian countries and its causative organism burkholderia pseudomallei is a recognized cause of pneumonia in these regions. recent isolation of the organism in the soil of kapasia, gazipur, bangladesh has proven its exposure among the population residing in endemic areas of our country. pneumonia is the most common presentation of melioidosis. acute, subacute and chronic pneumonia due to b. pseudomallei can present as primary or secondary ... | 2016 | 26879846 |
| predicted global distribution of burkholderia pseudomallei and burden of melioidosis. | burkholderia pseudomallei, a highly pathogenic bacterium that causes melioidosis, is commonly found in soil in southeast asia and northern australia(1,2). melioidosis can be difficult to diagnose due to its diverse clinical manifestations and the inadequacy of conventional bacterial identification methods(3). the bacterium is intrinsically resistant to a wide range of antimicrobials, and treatment with ineffective antimicrobials may result in case fatality rates (cfrs) exceeding 70%(4,5). the im ... | 2016 | 26877885 |
| lack of utility of nasopharyngeal swabs for diagnosis of burkholderia pseudomallei pneumonia in paediatric patients. | diagnosis of burkholderia pseudomallei pneumonia in children is challenging. we investigated the utility of nasopharyngeal swabs taken from 194 paediatric patients on admission with radiologically proven pneumonia. melioidosis was proven in 0.5% of samples tested and only in a third of those known to be bacteraemic with b. pseudomallei. it appears unlikely that culture of nasopharyngeal secretions is helpful to confirm b. pseudomallei pneumonia in paediatric patients. | 2016 | 26874977 |
| characterization of lesion formation in marmosets following inhalational challenge with different strains of burkholderia pseudomallei. | the marmoset model of melioidosis was used to explore whether there was any difference in the disease presentation and/or the lesion formation following inhalational challenge with one of four strains of burkholderia pseudomallei (k96243, 1026b, hbpub10303a and hbpub10134a). marmosets were challenged with a range of bacterial doses and bacterial load, histological and physiological features were determined temporally following lethal disease. melioidosis presented as an acute, febrile disease wi ... | 2015 | 26852689 |
| attenuation of a select agent-excluded burkholderia pseudomallei capsule mutant in hamsters. | burkholderia pseudomallei is a tier 1 select agent and potential bioweapon. given it is potential to cause a lethal respiratory disease, research with fully virulent b. pseudomallei is conducted in biosafety level 3 (bsl-3) laboratory spaces. the logistical, financial, and administrative burden of tier 1 select agent bsl-3 research has created an interest in mitigating such burdens through the use of either attenuated b. pseudomallei strains at bsl-2, or research with surrogate species, such as ... | 2016 | 26836271 |
| imported melioidosis in south korea: a case series with a literature review. | melioidosis is a potentially fatal infectious disease caused by the environmental anaerobic gram-negative bacillus burkholderia pseudomallei. melioidosis is endemic to areas of northern australia and southeast asia. with increasing international travel and migration, imported cases of melioidosis are being reported regularly. here, we summarize the 11 cases of melioidosis reported in south korea from 2003 to 2014. | 2015 | 26835246 |
| melioidosis acquired by a traveler from papua new guinea. | melioidosis is an infectious disease caused by burkholderia pseudomallei. melioidosis is of public health importance in endemic areas, particularly in tropical and sub-tropical areas. we describe a case of melioidosis contracted by a man with diabetes from papua new guinea that was evaluated using multi-locus sequence typing and whole genome sequencing. | 2017 | 26831499 |
| prevalence of bla(pena) and bla(oxa) in burkholderia pseudomallei isolated from patients at sappasitthiprasong hospital and their susceptibility to ceftazidime and carbapenems. | burkholderia pseudomallei is a causative agent of melioidosis. ceftazidime is the preferred drug of choice for treatment. however, the motility rate is high in endemic areas. | 2016 | 26817233 |
| relapsed melioidosis model in c57bl/6 mice. | burkholderia pseudomallei are the causative agents of melioidosis, a disease that has a high relapse rate in endemic areas. the mechanism of relapse is unclear | 2016 | 26817231 |
| intracellular replication of the well-armed pathogen burkholderia pseudomallei. | the burkholderia genus contains a group of soil-dwelling gram-negative organisms that are prevalent in warm and humid climates. two species in particular are able to cause disease in animals, b. mallei primarily infects equus spp. and b. pseudomallei (bps), that is able to cause potentially life-threatening disease in humans. bps is naturally resistant to many antibiotics and there is no vaccine available. although not a specialised human pathogen, bps possesses a large genome and many virulence ... | 2016 | 26803404 |
| neurological melioidosis in norway presenting with a cerebral abscess. | neurological melioidosis is a rare condition, as less than 30 cases have been reported in the last 50 years. we present a case of neurological melioidosis, presenting with a cerebral abscess in a returning traveler from an endemic area. while traveling in cambodia on holiday, the patient was admitted to local hospital for pneumonia. her condition improved after antimicrobial treatment, and she returned to norway when discharged. the patient had several contacts with the health care system after ... | 2015 | 26793441 |
| viperatoxin-ii: a novel viper venom protein as an effective bactericidal agent. | infections caused by methicillin-resistant staphylococcus aureus (mrsa) have become a rising threat to public health. there is an urgent need for development of promising new therapeutic agents against drug resistant bacteria like s. aureus. this report discusses purification and characterization of proteins from indian russell's viper snake venom. novel 15-kda proteins called "viperatoxin" (viptx-i and viptx-ii) were extracted from the whole venom and evaluated using in vitro antimicrobial expe ... | 2015 | 26793432 |
| incidental splenic granuloma due to burkholderia pseudomallei: a case of asymptomatic latent melioidosis? | we report a rare case of an asymptomatic latent melioidosis lesion in a posttraumatic splenectomy specimen from a diabetic patient. the 2-cm yellowish, lobulated lesion was found in the splenic parenchyma well away from the traumatized areas. microscopically, it consisted of a central area of necrosis and exudate surrounded by macrophages, epithelioid cells, lymphocytes, and occasional multinucleated giant cells. burkholderia bacilli were detected by a novel in situ hybridization (ish) assay, an ... | 2016 | 26787155 |
| a shelf-stable fluorogenic isothermal amplification assay for the detection of burkholderia pseudomallei. | a shelf-stable loop-mediated isothermal amplification (lamp) reagent for burkholderia pseudomallei detection is described. the coupling of lamp reagents with the indirect colorimetric indicator and consequently its lyophilization enable the simple evaluation of results without the need for any advance laboratory instruments. the reagents were found to have a stable shelf life of at least 30 days with well-maintained sensitivity and specificity. | 2016 | 26783560 |
| neurologic melioidosis: case report of a rare cause of acute flaccid paralysis. | acute flaccid paralysis is associated with inflammation, infection, or tumors in the spinal cord or peripheral nerves. melioidosis (burkholderia pseudomallei infection) can rarely cause this presentation. we describe a case of spinal melioidosis in a 4-year-old boy presenting with flaccid paralysis, and review the literature on this rare disease. | 2016 | 26778096 |
| burkholderia pseudomallei: its detection in soil and seroprevalence in bangladesh. | melioidosis, caused by burkholderia pseudomallei, is an endemic disease in bangladesh. no systematic study has yet been done to detect the environmental source of the organism and its true extent in bangladesh. the present study attempted to isolate b. pseudomallei in soil samples and to determine its seroprevalence in several districts in bangladesh. | 2016 | 26771511 |
| land use and soil type determine the presence of the pathogen burkholderia pseudomallei in tropical rivers. | burkholderia pseudomallei is the bacterium that causes melioidosis in humans. while b. pseudomallei is known to be endemic in south east asia (sea), the occurrence of the disease in other parts of the tropics points towards a potentially large global distribution. we investigated the environmental factors that influence the presence (and absence) of b. pseudomallei in a tropical watershed in sea. our main objective was to determine whether there is a link between the presence of the organism in ... | 2016 | 26758304 |
| ultrastructural effects and antibiofilm activity of lfchimera against burkholderia pseudomallei. | lactoferrin chimera (lfchimera), a hybrid peptide containing the two antimicrobial stretches of the innate immunity factor bovine lactoferrin, viz. lfampin265-284 and lfcin17-30, has strikingly high antimicrobial activity against the category b pathogen burkholderia pseudomallei. the action mechanisms of lfchimera against b. pseudomallei is not fully understood. the aim of this study was to further investigate the effect of treated b. pseudomallei with lfchimera using (immune) electron microscop ... | 2016 | 26754671 |
| multilocus sequence typing of 102 burkholderia pseudomallei strains isolated from china. | the phylogenetic and epidemiological relationships of 102 burkholderia pseudomallei clinical isolates from different geographical and population sources in china were investigated by multilocus sequence typing (mlst). the mlst data were analysed using the e-burst algorithm, and an unweighted pair-group method with arithmetic mean dendrogram was constructed based on the pair-wise differences in the allelic profiles of the strains. forty-one sequence types (sts) were identified, of which eight wer ... | 2016 | 26744829 |
| pulmonary melioidosis presenting with pleural effusion: a case report and review of literature. | melioidosis is a serious infection, which can involve multiple systems. we report a case of pulmonary melioidosis with the initial presentation mimicking a partially treated pneumonia complicated by right-sided pleural effusion. the patient is a 49-year old man who did not respond to parenteral ceftriaxone and tazobactam/piperacillin therapy. however, upon culture and sensitivity results from blood and pleural samples isolated burkholderia pseudomallei; antimicrobial therapy was de-escalated to ... | 2015 | 26744655 |
| burkholderia pseudomallei causing bone and joint infections: a clinical update. | burkholderia pseudomallei (b. pseudomallei), a causative agent of an emerging infectious disease melioidosis, is endemic in the tropical regions of the world. due to increased international travel, the infection is now also seen outside of the tropics. the majority of patients with identified risk factors such as diabetes mellitus, heavy alcohol use, malignancy, chronic lung and kidney disease, corticosteroid use, thalassemia, rheumatic heart disease, systemic lupus erythematosus and cardiac fai ... | 2016 | 26728713 |
| fatal bacteremic melioidosis in patients with prolonged neutropenia. | melioidosis, an infection with an expanding geographic range, is extremely rare in neutropenic patients. we report bacteremic melioidosis (st-70 and st-660) in 2 patients with prolonged neutropenia, who succumbed despite appropriate antibiotics. clinicians should be aware of this emerging infection in neutropenic patients residing in or returning from endemic areas. | 2016 | 26712267 |
| characterization of new virulence factors involved in the intracellular growth and survival of burkholderia pseudomallei. | burkholderia pseudomallei, the causative agent of melioidosis, has complex and poorly understood extracellular and intracellular lifestyles. we used transposon-directed insertion site sequencing (tradis) to retrospectively analyze a transposon library that had previously been screened through a balb/c mouse model to identify genes important for growth and survival in vivo. this allowed us to identify the insertion sites and phenotypes of negatively selected mutants that were previously overlooke ... | 2016 | 26712202 |
| membrane-bound pena β-lactamase of burkholderia pseudomallei. | burkholderia pseudomallei is the etiologic agent of melioidosis, a difficult-to-treat disease with diverse clinical manifestations. β-lactam antibiotics such as ceftazidime are crucial to the success of melioidosis therapy. ceftazidime-resistant clinical isolates have been described, and the most common mechanism is point mutations affecting expression or critical amino acid residues of the chromosomally encoded class a pena β-lactamase. we previously showed that pena was exported via the twin a ... | 2015 | 26711764 |
| atypical presentations of melioidosis in north india: report of two cases. | melioidosis is an infection caused by gram-negative bacterium, burkholderia pseudomallei. this is a rare disease in india, more so in north india. we present two cases of melioidosis with unusual sites of infection. the first patient was a young diabetic male presenting to us with history of prolonged fever and upper abdominal discomfort, subsequently diagnosed as a case of pyogenic liver abscess. the second patient was a middle aged diabetic complaining of prolonged fever and headache and found ... | 2015 | 26710411 |
| the in vitro antibiotic tolerant persister population in burkholderia pseudomallei is altered by environmental factors. | bacterial persistence due to antibiotic tolerance is a critical aspect of antibiotic treatment failure, disease latency, and chronic or reemergent infections. the levels of persisters is especially notable for the opportunistic gram-negative pathogens from the burkholderia and pseudomonas genera. we examined the rate of drug tolerant persisters in burkholderia pseudomallei, burkholderia thailandensis, burkholderia cepacia complex organisms, and pseudomonas aeruginosa at mid-log growth in lb brot ... | 2015 | 26696964 |
| anti-malarial and anti-inflammatory effects of gleichenia truncata mediated through inhibition of gsk3β. | gleichenia truncata is a highland fern from the gleicheniaceae family known for its traditional use among indigenous communities in asia to treat fever. the scientific basis of its effect has yet to be documented. a yeast-based kinase assay conducted in our laboratory revealed that crude methanolic extract (cme) of g. truncata exhibited glycogen synthase kinase-3 (gsk3)-inhibitory activity. gsk3β is now recognized to have a pivotal role in the regulation of inflammatory response during bacterial ... | 2015 | 26695202 |
| drug susceptibility and biofilm formation of burkholderia pseudomallei in nutrient-limited condition. | burkholderia pseudomallei is the causative agent of melioidosis, which can form biofilms and microcolonies in vivo and in vitro. one of the hallmark characteristics of the biofilm-forming bacteria is that they can be up to 1,000 times more resistant to antibiotics than their free-living counterpart. bacteria also become highly tolerant to antibiotics when nutrients are limited. one of the most important causes of starvation induced tolerance in vivo is biofilm growth. however, the effect of nutr ... | 2015 | 26691259 |
| differential toll-like receptor-signalling of burkholderia pseudomallei lipopolysaccharide in murine and human models. | the gram-negative bacterium burkholderia pseudomallei causes melioidosis and is a cdc category b bioterrorism agent. toll-like receptor (tlr)-2 impairs host defense during pulmonary b.pseudomallei infection while tlr4 only has limited impact. we investigated the role of tlrs in b.pseudomallei-lipopolysaccharide (lps) induced inflammation. purified b.pseudomallei-lps activated only tlr2-transfected-hek-cells during short stimulation but both hek-tlr2 and hek-tlr4-cells after 24 h. in human blood, ... | 2015 | 26689559 |
| unraveling the b. pseudomallei heptokinase wcbl: from structure to drug discovery. | gram-negative bacteria utilize heptoses as part of their repertoire of extracellular polysaccharide virulence determinants. disruption of heptose biosynthesis offers an attractive target for novel antimicrobials. a critical step in the synthesis of heptoses is their 1-o phosphorylation, mediated by kinases such as hlde or wcbl. here, we present the structure of wcbl from burkholderia pseudomallei. we report that wcbl operates through a sequential ordered bi-bi mechanism, loading the heptose firs ... | 2015 | 26687481 |
| novel engineered cationic antimicrobial peptides display broad-spectrum activity against francisella tularensis, yersinia pestis and burkholderia pseudomallei. | broad-spectrum antimicrobials are needed to effectively treat patients infected in the event of a pandemic or intentional release of a pathogen prior to confirmation of the pathogen's identity. engineered cationic antimicrobial peptides (ecaps) display activity against a number of bacterial pathogens including multi-drug-resistant strains. two lead ecaps, wlbu2 and wr12, were compared with human cathelicidin (ll-37) against three highly pathogenic bacteria: francisella tularensis, yersinia pesti ... | 2016 | 26673248 |
| accurate identification of members of the burkholderia cepacia complex in cystic fibrosis sputum. | the burkholderia cepacia complex (bcc) is a group of closely related species which includes opportunistic pathogens causing chronic respiratory infections in immunocompromised patients, or individuals affected by cystic fibrosis (cf). other burkholderia species causing infection in the cf population are burkholderia gladioli and burkholderia pseudomallei. traditional phenotypic analyses have been demonstrated to be inadequate for reliable identifications of isolates of bcc and b. gladioli. a pan ... | 2016 | 26671758 |
| first microbiota assessments of children's paddling pool waters evaluated using 16s rrna gene-based metagenome analysis. | insufficient chloric sterilization of children's paddling pool waters increases the risk of diarrheal illness. therefore, we investigated the microbiota changes after children use pools. first, we applied 16s rrna gene-based metagenome analysis to understand the dynamics of microbiota in pool water, especially with respect to the bio-contamination by potential pathogens. proteobacteria were major taxa detected in every pool water sample after children spent time in the pool. in more detail, gamm ... | 2017 | 26671497 |
| farnesol increases the susceptibility of burkholderia pseudomallei biofilm to antimicrobials used to treat melioidosis. | the aim of this study was to analyse the in vitro activity of farnesol alone and combined with the antibacterial drugs amoxicillin, doxycycline, ceftazidime and sulfamethoxazole-trimethoprim against burkholderia pseudomallei biofilms. | 2016 | 26669506 |
| utilization of whole-cell maldi-tof mass spectrometry to differentiate burkholderia pseudomallei wild-type and constructed mutants. | whole-cell matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (whole-cell maldi-tof ms) has been widely adopted as a useful technology in the identification and typing of microorganisms. this study employed the whole-cell maldi-tof ms to identify and differentiate wild-type and mutants containing constructed single gene mutations of burkholderia pseudomallei, a pathogenic bacterium causing melioidosis disease in both humans and animals. candidate biomarkers for the b. p ... | 2015 | 26656930 |
| burkholderia pseudomallei kynb plays a role in aq production, biofilm formation, bacterial swarming and persistence. | kynurenine formamidase (kynb) forms part of the kynurenine pathway which metabolises tryptophan to anthranilate. this metabolite can be used for downstream production of 2-alkyl-4-quinolone (aq) signalling molecules that control virulence in pseudomonas aeruginosa. here we investigate the role of kynb in the production of aqs and virulence-associated phenotypes of burkholderia pseudomallei k96243, the causative agent of melioidosis. deletion of kynb resulted in reduced aq production, increased b ... | 2016 | 26654915 |
| mechanisms of disease: host-pathogen interactions between burkholderia species and lung epithelial cells. | members of the burkholderia species can cause a range of severe, often fatal, respiratory diseases. a variety of in vitro models of infection have been developed in an attempt to elucidate the mechanism by which burkholderia spp. gain entry to and interact with the body. the majority of studies have tended to focus on the interaction of bacteria with phagocytic cells with a paucity of information available with regard to the lung epithelium. however, the lung epithelium is becoming more widely r ... | 2015 | 26636042 |
| finished annotated genome sequence of burkholderia pseudomallei strain bp1651, a multidrug-resistant clinical isolate. | burkholderia pseudomallei strain bp1651, a human isolate, is resistant to all clinically relevant antibiotics. we report here on the finished genome sequence assembly and annotation of the two chromosomes of this strain. this genome sequence may assist in understanding the mechanisms of antimicrobial resistance for this pathogenic species. | 2015 | 26634765 |
| an intriguing case of locked jaw secondary to melioidosis. | a 56-year-old woman presented with fever, pain and restriction of movement of the right temporomandibular joint. she was premorbidly diagnosed to have type 2 diabetes mellitus and rheumatoid arthritis. local examination revealed a poorly demarcated severely tender, erythematous swelling in the right preauricular region. all haematological and biochemical investigations were within normal limits. mri of the neck revealed the presence of a masticator space infection with intramuscular abscess invo ... | 2015 | 26628312 |
| structural analysis and knock-out of a burkholderia pseudomallei homolog of the eukaryotic transcription coactivator pc4. | homologs of the eukaryotic transcription coactivator pc4, which also functions in dna repair and oxidative stress, were recently identified in prokaryotes. crystallographic analysis of bpsl1147, a putative homolog from the pathogen burkholderia pseudomallei k96243, reveals a highly conserved core structure and suggests a nucleic acid binding mode similar to that of pc4. knock-out and complementation experiments do not reveal distinguishing phenotypes under normal growth conditions or in the pres ... | 2016 | 26625975 |
| the burkholderia pseudomallei proteins bapa and bapc are secreted ttss3 effectors and bapb levels modulate expression of bope. | many gram-negative pathogens use a type iii secretion system (ttss) for the injection of bacterial effector proteins into host cells. the injected effector proteins play direct roles in modulation of host cell pathways for bacterial benefit. burkholderia pseudomallei, the causative agent of melioidosis, expresses three different ttsss. one of these systems, the ttss3, is essential for escape from host endosomes and therefore intracellular survival and replication. here we have characterized thre ... | 2015 | 26624293 |
| neurologic melioidosis presented as encephalomyelitis and subdural collection in two male labourers in india. | two distinct and potentially deceitful cases of neurologic melioidosis are reported. case 1: a 39-year-old alcoholic and uncontrolled diabetic male presented with cough, fever, and left focal seizures with secondary generalization. an magnetic resonance imaging (mri) brain scan revealed a small peripherally enhancing subdural collection along the interhemispheric fissure suggestive of minimal subdural empyema. blood culture grew burkholderia pseudomallei. patient was diagnosed with disseminated ... | 2015 | 26623640 |
| sri lankan national melioidosis surveillance program uncovers a nationwide distribution of invasive melioidosis. | the epidemiologic status of melioidosis in sri lanka was unclear from the few previous case reports. we established laboratory support for a case definition and started a nationwide case-finding study. suspected burkholderia pseudomallei isolates were collated, identified by polymerase chain reaction assay, referred for matrix assisted laser desorption ionization-time of flight analysis and multilocus sequence typing (mlst), and named according to the international mlst database. between 2006 an ... | 2016 | 26621560 |
| burkholderia pseudomallei is frequently detected in groundwater that discharges to major watercourses in northern australia. | burkholderia pseudomallei is the environmental bacterium that causes the serious disease melioidosis. recently, a high prevalence of viable b. pseudomallei was reported from natural groundwater seeps around castle hill, a clinical focus of melioidosis in townsville, australia. this study sought to expand previous findings to determine the extent of b. pseudomallei in more diverse natural groundwater seeps in northern queensland to ascertain if the presence of the organism in groundwater on castl ... | 2016 | 26620184 |
| distinct colicin m-like bacteriocin-immunity pairs in burkholderia. | the escherichia coli bacteriocin colicin m (colm) acts via degradation of the cell wall precursor lipid ii in target cells. colm producers avoid self-inhibition by a periplasmic immunity protein anchored in the inner membrane. in this study, we identified colm-like bacteriocin genes in genomes of several β-proteobacterial strains belonging to the burkholderia cepacia complex (bcc) and the burkholderia pseudomallei group. two selected burkholderia ambifaria proteins, designated burkhocins m1 and ... | 2015 | 26610609 |
| unprecedented melioidosis cases in northern australia caused by an asian burkholderia pseudomallei strain identified by using large-scale comparative genomics. | melioidosis is a disease of humans and animals that is caused by the saprophytic bacterium burkholderia pseudomallei. once thought to be confined to certain locations, the known presence of b. pseudomallei is expanding as more regions of endemicity are uncovered. there is no vaccine for melioidosis, and even with antibiotic administration, the mortality rate is as high as 40% in some regions that are endemic for the infection. despite high levels of recombination, phylogenetic reconstruction of ... | 2016 | 26607593 |
| diverse burkholderia species isolated from soils in the southern united states with no evidence of b. pseudomallei. | the global distribution of the soil-dwelling bacterium burkholderia pseudomallei, causative agent of melioidosis, is poorly understood. we used established culturing methods developed for b. pseudomallei to isolate burkholderia species from soil collected at 18 sampling sites in three states in the southern united states (arizona (n = 4), florida (n = 7), and louisiana (n = 7)). using multi-locus sequence typing (mlst) of seven genes, we identified 35 burkholderia isolates from these soil sample ... | 2015 | 26600238 |
| characterization of burkholderia pseudomallei protein bpsl1375 validates the putative hemolytic activity of the cog3176 n-acyltransferase family. | there are still numerous protein subfamilies within families and superfamilies that do not yet have conclusive empirical experimental evidence providing a specific function. these proteins persist in databases with the annotation of a specific 'putative' function made by association with discernible features in the protein sequence. | 2015 | 26597807 |
| exposing a β-lactamase "twist": the mechanistic basis for the high level of ceftazidime resistance in the c69f variant of the burkholderia pseudomallei peni β-lactamase. | around the world, burkholderia spp. are emerging as pathogens highly resistant to β-lactam antibiotics, especially ceftazidime. clinical variants of burkholderia pseudomallei possessing the class a β-lactamase peni with substitutions at positions c69 and p167 are known to demonstrate ceftazidime resistance. however, the biochemical basis for ceftazidime resistance in class a β-lactamases in b. pseudomallei is largely undefined. here, we performed site saturation mutagenesis of the c69 position a ... | 2016 | 26596949 |
| gene microarray analyses of daboia russelli russelli daboiatoxin treatment of thp-1 human macrophages infected with burkholderia pseudomallei. | burkholderia pseudomallei is the causative agent of melioidosis and represents a potential bioterrorism threat. in this study, the transcriptomic responses of b. pseudomallei infection of a human macrophage cell model were investigated using whole-genome microarrays. gene expression profiles were compared between infected thp-1 human monocytic leukemia cells with or without treatment with daboia russelli russelli daboiatoxin (drrdbtx) or ceftazidime (antibiotic control). microarray analyses of i ... | 2015 | 26592245 |
| genomic sequence of burkholderia multivorans nki379, a soil bacterium that inhibits the growth of burkholderia pseudomallei. | burkholderia multivorans nki379 is a soil bacterium that exhibits an antagonistic effect against the growth of burkholderia pseudomallei, the causative agent of the infectious disease melioidosis. we report the draft genomic sequence of b. multivorans nki379, which has a g+c content of 67% and 5,203 candidate protein-encoding genes. | 2015 | 26586873 |