Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| Virological and clinical characterization of respiratory infections in children attending an emergency department during the first autumn-winter circulation of pandemic A (H1N1) 2009 influenza virus. | Clin Microbiol Infect ABSTRACT: To characterize respiratory virus infections during the first autumn-winter season of pandemic A (H1N1) 2009 influenza virus (A/H1N1/2009) circulation, a prospective study in children attending a paediatric emergency department at the Sapienza University hospital, Rome, was conducted from November 2009 to March 2010. By means of both nasal washings and pharyngeal swabs, enrolled children were checked for 14 respiratory viruses. The majority of acute respiratory in ... | 2011 | 21923780 |
| Effectiveness of palivizumab prophylaxis in infants and children in Florida. | PURPOSE: Palivizumab effectiveness data on respiratory syncytial virus (RSV) infections are limited to trial settings and vary considerably between selected high-risk populations. This study aimed to evaluate effectiveness in a community-based sample. METHODS: We conducted a cohort study of children with =3?months Florida Medicaid fee-for-service eligibility between 1998 and 2004 who also had matching birth certificates. Children entered the cohort at the beginning of the RSV season, after a min ... | 2011 | 21919115 |
| th17 cytokines are critical for respiratory syncytial virus-associated airway hyperreponsiveness through regulation by complement c3a and tachykinins. | respiratory syncytial virus (rsv) infection is associated with serious lung disease in infants and immunocompromised individuals and is linked to development of asthma. in mice, acute rsv infection causes airway hyperresponsiveness (ahr), inflammation, and mucus hypersecretion. infected cells induce complement activation, producing the anaphylatoxin c3a. in this paper, we show rsv-infected wild-type mice produce th17 cytokines, a response not previously associated with viral infections. mice def ... | 2011 | 21918196 |
| repetitive prime-and-realign mechanism converts short capped rna leaders into longer ones that may be more suitable for elongation during rice stripe virus transcription initiation. | cucumber mosaic virus (cmv) rnas were found to serve as cap donors for rice stripe virus (rsv) transcription initiation during their co-infection of nicotiana benthamiana. the 5' end of cmv rnas was cleaved preferentially at residues that had multiple-base complementarity to the 3' end of the rsv template. the length requirement for cmv capped primers to be suitable for elongation varied between 12 and 20 nt, and those of 12-16 nt were optimal for elongation and generated more cmv-rsv chimeric m ... | 2012 | 21918010 |
| sequences in gibbon ape leukemia virus envelope that confer sensitivity to hiv-1 accessory protein vpu. | hiv-1 efficiently forms pseudotyped particles with many gammaretrovirus glycoproteins, such as friend murine leukemia virus (f-mlv) env, but not with the related gibbon ape leukemia virus (galv) env or with a chimeric f-mlv env with a galv cytoplasmic tail domain (ctd). this incompatibility is modulated by the hiv-1 accessory protein vpu. because the galv env ctd does not resemble tetherin or cd4, the well-studied targets of vpu, we sought to characterize the modular sequence in the galv env ctd ... | 2011 | 21917962 |
| evaluation of protective efficacy of respiratory syncytial virus vaccine against a and b subgroup human isolates in korea. | human respiratory syncytial virus (hrsv) is a significant cause of upper and lower respiratory tract illness mainly in infants and young children worldwide. hrsv is divided into two subgroups, hrsv-a and hrsv-b, based on sequence variation within the g gene. despite its importance as a respiratory pathogen, there is currently no safe and effective vaccine for hrsv. in this study, we have detected and identified the hrsv by rt-pcr from nasopharyngeal aspirates of korean pediatric patients. intere ... | 2011 | 21915262 |
| [a guinea pig model of respiratory syncytial virus infection for cough and its neurogenic inflammatory mechanism]. | to establish a guinea pig model of acute and postinfectious cough caused by respiratory syncytial virus (rsv) infection and investigate the role of neurogenic inflammation in its pathogenesis. | 2011 | 21914323 |
| autophagy-mediated dendritic cell activation is essential for innate cytokine production and apc function with respiratory syncytial virus responses. | the regulation of innate immune responses during viral infection is a crucial step to promote antiviral reactions. recent studies have drawn attention to a strong relationship of pathogen-associated molecular pattern recognition with autophagy for activation of apc function. our initial observations indicated that autophagosomes formed in response to respiratory syncytial virus (rsv) infection of dendritic cells (dc). to further investigate whether rsv-induced dc activation and innate cytokine p ... | 2011 | 21911604 |
| local interleukin-10 production during respiratory syncytial virus bronchiolitis is associated with post-bronchiolitis wheeze. | respiratory syncytial virus (rsv) is the most common cause of bronchiolitis in infants. following rsv bronchiolitis, 50% of children develop post-bronchiolitis wheeze (pbw). animal studies have suggested that interleukin (il)-10 plays a critical role in the pathogenesis of rsv bronchiolitis and subsequent airway hyperresponsiveness. previously, we showed that ex vivo monocyte il-10 production is a predictor of pbw. additionally, heterozygosity of the single-nucleotide polymorphism (snp) rs180087 ... | 2011 | 21910858 |
| equal virulence of rhinovirus and respiratory syncytial virus in infants hospitalized for lower respiratory tract infection. | respiratory syncytial virus (rsv) and rhinovirus (rv) are predominant viruses associated with lower respiratory tract infection in infants. we compared the symptoms of lower respiratory tract infection caused by rsv and rv in hospitalized infants. rv showed the same symptoms as rsv, so on clinical grounds, no difference can be made between these pathogens. no relation between polymerase chain reaction cycle threshold value and length of hospital stay was found. | 2011 | 21909047 |
| Evidence for a causal relationship between respiratory syncytial virus infection and asthma. | Respiratory syncytial virus (RSV) infects all children early in life, is the most common cause of infant lower respiratory tract infections, and causes disease exacerbations in children with asthma. Episodes of lower respiratory tract infection in early life are associated with asthma development. Whether RSV infection early in life directly causes asthma or simply identifies infants who are genetically predisposed to develop subsequent wheezing is debatable. Recent studies suggest that these tw ... | 2011 | 21905783 |
| impact of respiratory syncytial virus: the nurse's perspective. | respiratory syncytial virus (rsv) is a highly contagious virus, and is the major cause of lower respiratory tract infections in infants and toddlers worldwide. rsv infection poses serious health risks to young children during the first 2 years of life. several infant populations have been classified as high risk, and additional risk factors are known to increase the likelihood of severe rsv infection. treatment for active rsv infection is limited to the symptoms of infection rather than the unde ... | 2011 | 21902285 |
| Infection with multiple viruses is not associated with increased disease severity in children with bronchiolitis. | BACKGROUND: The clinical relevance of parallel detection of multiple viruses by real-time polymerase chain reaction (RT-PCR) remains unclear. This study evaluated the association between the detection of multiple viruses by RT-PCR and disease severity in children with bronchiolitis. METHODS: Children less than 2 years of age with clinical symptoms of bronchiolitis were prospectively included during three winter seasons. Patients were categorized in three groups based on disease severity; mild (n ... | 2011 | 21901859 |
| Viral aetiology of influenza-like illness in Belgium during the influenza A(H1N1)2009 pandemic. | The purpose of this investigation was to determine the proportion of influenza-like illness (ILI) attributable to specific viruses during the influenza A(H1N1)2009 pandemic and to describe the demographic and clinical characteristics of ILI due to respiratory viruses in Belgium. Nasopharyngeal swabs were collected from ILI patients by general practitioners (GPs) and paediatricians (PediSurv) and analysed for viruses. Of 139 samples collected from children <5 years of age by PediSurv, 86 were pos ... | 2011 | 21901635 |
| viral infection induces expression of novel phased micrornas from conserved cellular microrna precursors. | rna silencing, mediated by small rnas including micrornas (mirnas) and small interfering rnas (sirnas), is a potent antiviral or antibacterial mechanism, besides regulating normal cellular gene expression critical for development and physiology. to gain insights into host small rna metabolism under infections by different viruses, we used solexa/illumina deep sequencing to characterize the small rna profiles of rice plants infected by two distinct viruses, rice dwarf virus (rdv, dsrna virus) and ... | 2011 | 21901091 |
| respiratory syncytial virus--united states, july 2007-june 2011. | each year in the united states, an estimated 75,000-125,000 hospitalizations related to respiratory syncytial virus (rsv)occur among children aged <1 year, and rsv infection results in approximately 1.5 million outpatient visits among children aged <5 years. in the united states, rsv season begins in the fall, peaks in winter, and ends in the late winter and early spring. however, the exact timing and duration vary from year to year and by geographic region. to describe trends in rsv seasonality ... | 2011 | 21900874 |
| viral-bacterial interactions and risk of acute otitis media complicating upper respiratory tract infection. | acute otitis media (aom) is a common complication of upper respiratory tract infection whose pathogenesis involves both viruses and bacteria. we examined risks of acute otitis media associated with specific combinations of respiratory viruses and acute otitis media bacterial pathogens. data were from a prospective study of children ages 6 to 36 months and included viral and bacterial culture and quantitative pcr for respiratory syncytial virus (rsv), human bocavirus, and human metapneumovirus. r ... | 2011 | 21900518 |
| rela ser276 phosphorylation-coupled lys310 acetylation controls transcriptional elongation of inflammatory cytokines in respiratory syncytial virus infection. | respiratory syncytial virus (rsv) is a negative-sense single-stranded rna virus responsible for lower respiratory tract infections (lrtis) in humans. in experimental models of rsv lrti, the actions of the nuclear factor κb (nf-κb) transcription factor mediate inflammation and pathology. we have shown that rsv replication induces a mitogen-and-stress-related kinase 1 (msk-1) pathway that activates nf-κb rela transcriptional activity by a process involving serine phosphorylation at serine (ser) re ... | 2011 | 21900162 |
| effect of respiratory syncytial virus infection on plasmacytoid dendritic cell regulation of allergic airway inflammation. | respiratory syncytial virus (rsv) can infect myeloid dendritic cells (mdcs) and regulate their function in the development of allergy. it has been widely reported that plasmacytoid dcs (pdcs) play a critical role in antiviral innate immunity. in contrast, not much is known about the role of pdcs in the interaction between allergy and viral infection. the purpose of the present study was to investigate the effect of rsv infection on pdc function in the regulation of allergic airway inflammation i ... | 2012 | 21894025 |
| Central role of dendritic cells in shaping the adaptive immune response during respiratory syncytial virus infection. | Respiratory syncytial virus (RSV) is the leading cause of lower respiratory tract disease in young children. Premature infants, immunocompromised individuals and the elderly exhibit the highest risk for the development of severe RSV-induced disease. Murine studies demonstrate that CD8 T cells mediate RSV clearance from the lungs. Murine studies also indicate that the host immune response contributes to RSV-induced morbidity as T-cell depletion prevents the development of disease despite sustaine ... | 2011 | 21887154 |
| respiratory syncytial virus load, viral dynamics, and disease severity in previously healthy naturally infected children. | respiratory syncytial virus (rsv) disease severity was thought to be a result of host immunopathology but alternatively may be driven by high-level viral replication. the relationships between rsv load, viral clearance dynamics, and disease severity have not been carefully evaluated. | 2011 | 21881113 |
| Viruslike particle vaccine induces protection against respiratory syncytial virus infection in mice. | Respiratory syncytial virus (RSV) is the leading cause of bronchiolitis and viral death in infants. Despite decades of research with traditional or subunit vaccine approaches, there are no approved RSV vaccines. New approaches are therefore urgently needed to develop effective RSV vaccines. | 2011 | 21881112 |
| The first two nucleotides of the respiratory syncytial virus antigenome RNA replication product can be selected independently of the promoter terminus. | There is limited knowledge regarding how the RNA-dependent RNA polymerases of the nonsegmented negative-strand RNA viruses initiate genome replication. In a previous study of respiratory syncytial virus (RSV) RNA replication, we found evidence that the polymerase could select the 5'-ATP residue of the genome RNA independently of the 3' nucleotide of the template. To investigate if a similar mechanism is used during antigenome synthesis, a study of initiation from the RSV leader (Le) promoter was ... | 2011 | 21878549 |
| Wheezing lower respiratory disease and vaccination of premature infants. | Premature infants are at increased risk of wheezing in association with respiratory syncytial virus (RSV) and rhinovirus infections. We assess possible associations between wheezing and routine vaccinations of premature infants. | 2011 | 21875634 |
| flavone c-glycosides from the leaves of lophatherum gracile and their in vitro antiviral activity. | four new flavone c-glycosides, luteolin 6- c- α-l-arabinopyranosyl-7- o- β-d-glucopyranoside ( 1), apigenin 6- c- β-d-galactopyranosiduronic acid (1 → 2)- α-l-arabinopyranoside ( 2), luteolin 6- c- β-d-galactopyranosiduronic acid (1 → 2)- α-l-arabinopyranoside ( 3), and luteolin 6- c- β-d-glucopyranosiduronic acid (1 → 2)- α-l-arabinofuranoside ( 4), along with three known ones, were isolated from the leaves of lophatherum gracile. the structures of the new compounds were elucidated by extensi ... | 2011 | 21870321 |
| seasonal variations of 15 respiratory agents illustrated by the application of a multiplex polymerase chain reaction assay. | abstract background: nucleic acid amplification tests are increasingly being used to diagnose viral and bacterial respiratory tract infections. the high sensitivity of these tests affects our understanding of the epidemiology of respiratory tract infections. we have assessed the detection rate of a multiplex real-time polymerase chain reaction (pcr) test, with emphasis on epidemiology and seasonal distribution of the most common respiratory tract infections. methods: seven thousand eight hundr ... | 2012 | 21867470 |
| respiratory syncytial virus infection and the tight junctions of nasal epithelial cells. | respiratory syncytial virus (rsv) primarily infects upper respiratory tract cells, mainly nasal epithelial cells. the tight junctions of nasal epithelial cells are thought to perform important innate immune function against foreign materials including respiratory viruses. we investigated in vitro the relationship of rsv infection and the tight junctions of primary nasal epithelial cells which had been transfected with human telomerase reverse transcriptase (htert) to prolong cell life. nasal epi ... | 2011 | 21865717 |
| Primary airway epithelial cultures from children are highly permissive to respiratory syncytial virus infection. | Background Respiratory syncytial virus (RSV) infection of airway epithelial cells (AECs) is an important initial event in RSV bronchiolitis. AEC immunological responses are thought to be critical in driving the subsequent inflammation in the airway. This study examined viral replication, cytotoxicity and cytokine production in cultures of primary AECs from children compared with responses to RSV infection in an immortalised epithelial cell line and to those from infants with RSV bronchiolitis. ... | 2012 | 21865207 |
| rna viruses in young nepalese children hospitalized with severe pneumonia. | pneumonia is among the leading causes of illness and death in children <5 years of age worldwide. there is little information on the viral etiology of severe pneumonia in low-income countries, where the disease burden is particularly high. | 2011 | 21860338 |
| progress in respiratory virus vaccine development. | viral respiratory infections cause significant morbidity and mortality in infants and young children as well as in at-risk adults and the elderly. although many viral pathogens are capable of causing respiratory disease, vaccine development has to focus on a limited number of pathogens, such as those that commonly cause serious lower respiratory illness (lri). whereas influenza virus vaccines have been available for some time (see the review by clark and lynch in this issue), vaccines against ot ... | 2011 | 21858754 |
| Respiratory viral infections in hematopoietic stem cell and solid organ transplant recipients. | Respiratory viral infections (RVIs) are common causes of mild illness in immunocompetent children and adults with rare occurrences of significant morbidity or mortality. Complications are more common in the very young, very old, and those with underlying lung diseases. However, RVIs are increasingly recognized as a cause of morbidity and mortality in recipients of hematopoietic stem cell transplants (HSCT) and solid organ transplants (SOTs). Diagnostic techniques for respiratory syncytial virus ... | 2011 | 21858751 |
| respiratory syncytial virus infection in adults. | respiratory syncytial virus (rsv), an enveloped rna virus in the paramyxovirus family, is the most important cause of lower respiratory tract infection in infants and young children, accounting for ~100,000 pediatric hospitalizations and 250 deaths annually in the united states. despite primarily being recognized as a pediatric pathogen, rsv reinfection causes substantial disease in all adult populations, including healthy young persons, old and frail individuals, those with chronic obstructive ... | 2011 | 21858747 |
| a crystal structure of the catalytic core domain of an avian sarcoma and leukemia virus integrase suggests an alternate dimeric assembly. | integrase (in) is an important therapeutic target in the search for anti-human immunodeficiency virus (hiv) inhibitors. this enzyme is composed of three domains and is hard to crystallize in its full form. first structural results on in were obtained on the catalytic core domain (ccd) of the avian rous and sarcoma virus strain schmidt-ruppin a (rsv-a) and on the ccd of hiv-1 in. a ribonuclease-h like motif was revealed as well as a dimeric interface stabilized by two pairs of α-helices (α1/α5, α ... | 2011 | 21857987 |
| Non-invasive sample collection for respiratory virus testing by multiplex PCR. | Identifying respiratory pathogens within populations is difficult because invasive sample collection, such as with nasopharyngeal aspirate (NPA), is generally required. PCR technology could allow for non-invasive sampling methods. | 2011 | 21855405 |
| identification of potential human respiratory syncytial virus and metapneumovirus t cell epitopes using computational prediction and mhc binding assays. | human respiratory syncytial virus (rsv) and human metapneumovirus (mpv) are two of the most common causes of serious viral lower respiratory tract illness in humans. cd8+ t cells have been shown to be important in animal models and human clinical studies for the clearance of viral infection, and they may contribute in part to protection against severe disease during reinfections. precise enumeration and accurate phenotyping of rsv- or mpv-specific cd8+ t cells in humans is currently limited by t ... | 2011 | 21854782 |
| RSV infection modulates IL-15 production and MICA levels in respiratory epithelial cells. | The cytokine interleukin-15 (IL-15), major histocompatibility complex (MHC) class I molecules and MHC class I chain-related proteins (MIC) A and B (MICA/B) are involved in cellular immune responses to virus infections but their role in respiratory syncytial virus (RSV) infection has not been studied. We aimed to determine how RSV infection modulates IL-15 production, MHC class I and MICA expression in respiratory epithelial cells, the molecular pathways implicated in virus-induced IL-15 producti ... | 2011 | 21852331 |
| juxtaposition of two distant, serine-arginine-rich protein-binding elements is required for optimal polyadenylation in rous sarcoma virus. | the rous sarcoma virus (rsv) polyadenylation site (pas) is very poorly used in vitro due to suboptimal upstream and downstream elements, and yet ∼85% of viral transcripts are polyadenylated in vivo. the mechanisms that orchestrate polyadenylation at the weak pas are not completely understood. it was previously shown that serine-arginine (sr)-rich proteins stimulate rsv pas use in vitro and in vivo. it has been proposed that viral rna polyadenylation is stimulated through a nonproductive splice c ... | 2011 | 21849435 |
| Krüppel-like factor 6 regulates transforming growth factor-ß gene expression during human respiratory syncytial virus infection. | Human respiratory syncytial virus (RSV) infection is associated with airway remodeling and subsequent asthma development. Transforming growth factor-beta (TGF) plays a crucial role in asthma development. The mechanism regulating TGF gene expression during RSV infection is not known. Kruppel-like factor family of transcription factors are critical regulators of cellular/tissue homeostasis. Previous studies have shown that Kruppel-like factor 6 (KLF6) could function as a trans-activator of TGF gen ... | 2011 | 21849067 |
| multiple cd4+ t cell subsets produce immunomodulatory il-10 during respiratory syncytial virus infection. | the host immune response is believed to contribute to the severity of pulmonary disease induced by acute respiratory syncytial virus (rsv) infection. because rsv-induced pulmonary disease is associated with immunopathology, we evaluated the role of il-10 in modulating the rsv-specific immune response. we found that il-10 protein levels in the lung were increased following acute rsv infection, with maximum production corresponding to the peak of the virus-specific t cell response. the majority of ... | 2011 | 21844390 |
| identification of nucleolin as a cellular receptor for human respiratory syncytial virus. | human respiratory syncytial virus (rsv) causes a large burden of disease worldwide. there is no effective vaccine or therapy, and the use of passive immunoprophylaxis with rsv-specific antibodies is limited to high-risk patients. the cellular receptor (or receptors) required for viral entry and replication has yet to be described; its identification will improve understanding of the pathogenesis of infection and provide a target for the development of novel antiviral interventions. here we show ... | 2011 | 21841784 |
| confirmation of an association between single nucleotide polymorphisms in the vdr gene with respiratory syncytial virus related disease in south african children. | respiratory syncytial virus is a leading cause of lower respiratory tract infection in infants. disease severity has been linked to host immune responses and polymorphisms in genes associated with innate immunity. a large-scale genetics study of single nucleotide polymorphisms (snps) in children in the netherlands identified snps in the vitamin d receptor (vdr) and jun genes which have a strong association with an increased risk of developing bronchiolitis following the first respiratory syncyti ... | 2011 | 21837802 |
| antibody response and avidity of respiratory syncytial virus-specific total igg, igg1, and igg3 in young children. | respiratory syncytial virus (rsv) is a major cause of acute respiratory disease in infants and young children. considering that several aspects of the humoral immune response to rsv infection remain unclear, this study aimed to investigate the occurrence, levels, and avidity of total igg, igg1, and igg3 antibodies against rsv in serum samples from children ôëñ5 years old. in addition, a possible association between antibody avidity and severity of illness was examined. the occurrence and levels ... | 2011 | 21837801 |
| tak1 regulates nf-+üb and ap-1 activation in airway epithelial cells following rsv infection. | respiratory syncytial virus (rsv) is the most common cause of epidemic respiratory diseases in infants and young children. rsv infection of airway epithelial cells induces the expression of immune/inflammatory genes through the activation of a subset of transcription factors, including nuclear factor-+¦b (nf-+¦b) and ap-1. in this study, we have investigated the signaling pathway leading to activation of these two transcription factors in response to rsv infection. our results show that ikk+¦ pl ... | 2011 | 21835421 |
| a review of palivizumab and emerging therapies for respiratory syncytial virus. | introduction: respiratory syncytial virus (rsv) is an important pathogen in children and adults; however, current treatment options are primarily supportive. palivizumab, the only approved specific monoclonal antibody for rsv is used prophylactically to reduce morbidity in a select population of high-risk children. areas covered: the development and current use of palivizumab; the potential role of palivizumab as preventive therapy in patients with cystic fibrosis, asthma and compromised immune ... | 2011 | 21831008 |
| respiratory syncytial virus prevention in children with congenital heart disease: who and how? | respiratory syncytial virus (rsv) is a major cause of respiratory infection in children. most of the pediatric population have rsv infection before the age of 2, and recurrent infections are common even within one season. chronic lung disease, prematurity, along with congenital heart disease (chd) are major risk factors in severe lower respiratory infection. in hemo-dynamically significant chd patients with rsv infection, hospitalization is usually needed and the possibility of treatment in inte ... | 2011 | 21829410 |
| respiratory syncytial virus infection in children with congenital heart disease: global data and interim results of korean rsv-chd survey. | respiratory syncytial virus (rsv) is a main cause of hospitalization for bronchiolitis and pneumonia in infants worldwide. children with hemodynamically significant congenital heart disease (hs-chd), as well as premature infants are at high risk for severe rsv diseases. mortality rates for chd patients hospitalized with rsv have been reported as about 24 times higher compared with those without rsv infection. recently with advances in intensive care, mortality rates in chd patients combined with ... | 2011 | 21829409 |
| autocrine regulation of pulmonary inflammation by effector t-cell derived il-10 during infection with respiratory syncytial virus. | respiratory syncytial virus (rsv) infection is the leading viral cause of severe lower respiratory tract illness in young infants. clinical studies have documented that certain polymorphisms in the gene encoding the regulatory cytokine il-10 are associated with the development of severe bronchiolitis in rsv infected infants. here, we examined the role of il-10 in a murine model of primary rsv infection and found that high levels of il-10 are produced in the respiratory tract by anti-viral effect ... | 2011 | 21829368 |
| decreased innate immune cytokine responses correlate with disease severity in children with respiratory syncytial virus and human rhinovirus bronchiolitis. | the immunopathogenesis of respiratory syncytial virus (rsv) and human rhinovirus lower respiratory tract infections in children remains to be defined. we measured nasal wash concentrations of 29 cytokines in infants with rsv or human rhinovirus lower respiratory tract infections. concentrations of interferon-+¦ in rsv and innate immunity cytokines in both infections inversely correlated with disease severity. | 2011 | 21829141 |
| RSV 2010: Recent advances in research on respiratory syncytial virus and other pneumoviruses. | Respiratory syncytial virus (RSV) and human metapneumovirus (HMPV) are important causes of acute respiratory tract disease in infants, immunocompromised patients and the elderly. The Seventh International RSV symposium was held in Rotterdam, the Netherlands, from December 2-5, 2010. This symposium is the flagship event for leading investigators engaged in RSV and HMPV research around the world. The objective of the symposium was to provide a forum to review recent advances in research on RSV, HM ... | 2011 | 21827813 |
| respiratory syncytial virus infection is associated with an altered innate immunity and a heightened pro-inflammatory response in the lungs of preterm lambs. | abstract: | 2011 | 21827668 |
| respiratory syncytial virus-associated hospitalizations among infants and young children in the united states, 1997-2006. | background:: respiratory syncytial virus (rsv) is the most common cause of lower respiratory tract disease among young children in the united states. rsv-associated hospitalization increased among children in the united states during 1980 through 1996. in this study, we updated national estimates of rsv hospitalization rates among us children through 2006. methods:: we conducted a retrospective analysis of hospital discharges for lower respiratory tract illness (lrti) in children <5 years old fr ... | 2011 | 21817948 |
| type-iii interferon, not type-i, is the predominant interferon induced by respiratory viruses in nasal epithelial cells. | as an innate immune response against diverse viral infections, a host induces two types of interferon (ifn), type-i (ifn-+¦/+¦) and type-iii (ifn-++). we investigated ifn inductions by respiratory viruses, including respiratory syncytial virus (rsv), measles virus and mumps virus in human nasal epithelial cells (necs). ifn-++, but not ifn-+¦/+¦, was induced by respiratory virus infection in primary necs and immortalized necs through transfection with the human telomerase reverse transcriptase ge ... | 2011 | 21816185 |
| expression of an rsv-gag virus-like particle in insect cell lines and silkworm larvae. | rous sarcoma virus group antigen protein-based virus-like particles (vlps) are well known for their structural integrity and ease of handling. vlps play an important role in drug delivery systems because they can be manipulated with ease. in this study, a new method was established for expressing rous sarcoma virus group antigen protein based vlps in silkworm larvae and establishing stably expressing insect cell lines. these vlps have been isolated by ultracentrifugation using a sucrose step gra ... | 2011 | 21816175 |
| il10 family member genes il19 and il20 are associated with recurrent wheeze after respiratory syncytial virus bronchiolitis. | mechanisms underlying the increased risk of recurrent wheeze following respiratory syncytial virus lower respiratory tract infection (rsv lrti) are unclear. specifically, information about genetic determinants of recurrent wheeze following rsv lrti is limited. we performed a candidate gene association study to identify genetic determinants of recurrent wheeze following rsv lrti. we investigated 346 single nucleotide polymorphisms (snps) in 220 candidate genes in 166 dutch infants hospitalized fo ... | 2011 | 21814157 |
| the early history of tumor virology: rous, rif, and rav. | one hundred years ago peyton rous recovered a virus, now known as the rous sarcoma virus (rsv), from a chicken sarcoma, which reproduced all aspects of the tumor on injection into closely related chickens. there followed recovery of causal viruses of tumors of different morphology from 4 more of 60 chicken tumors. subsequent studies in chickens of the biology of the first rsv isolated moved slowly for 45 y until an assay of ectodermal pocks of the chorioallantoic membrane of chicken embryos was ... | 2011 | 21813762 |
| rous sarcoma virus gag has no specific requirement for phosphatidylinositol-(4,5)-bisphosphate for plasma membrane association in vivo or for liposome interaction in vitro. | the ma domain of the retroviral gag protein mediates interactions with the plasma membrane, which is the site of productive virus release. hiv-1 ma has a phosphatidylinositol- (4, 5)-bisphosphate [pi(4,5)p(2)] binding pocket; depletion of this phospholipid from the plasma membrane compromises gag membrane association and virus budding. we used multiple methods to examine the possible role of pi(4,5)p(2) in gag-membrane interaction of the alpharetrovirus rous sarcoma virus (rsv). in contrast to h ... | 2011 | 21813603 |
| household tobacco smoke and admission weight predict severe bronchiolitis in infants independent of deprivation: prospective cohort study. | to examine demographic, environmental and clinical factors associated with severe bronchiolitis in infants admitted to hospital and quantify the independent effects of these factors. | 2011 | 21811609 |
| innate immunity proteins and a new truncated form of splunc1 in nasopharyngeal aspirates from infants with respiratory syncytial virus infection. | purpose: respiratory syncytial virus (rsv) is the most common cause of severe respiratory tract infection in infants. the aim was to identify host defence components in nasopharyngeal aspirate (npa) from infants with rsv infection and to study the expression of the novel 25 kda innate immunity protein splunc1. experimental design: npas from infants were analyzed with 2-de and ms in a pilot study. the levels of splunc1 were analyzed with immunoblotting in 47 npas, admitted for rsv diagnosis. resu ... | 2011 | 21805676 |
| absence of vaccine-enhanced rsv disease and changes in pulmonary dendritic cells with adenovirus-based rsv vaccine. | abstract: the development of a vaccine against respiratory syncytial virus (rsv) has been hampered by the risk for vaccine-enhanced rsv pulmonary disease induced by immunization with formalin-inactivated rsv (firsv). this study focuses on the evaluation of vaccine-enhanced pulmonary disease following immunization with adf.rgd, an integrin-targeted adenovirus vector that expresses the rsv f protein and includes an rgd (arg-gly-asp) motif. immunization of balb/c mice with adf.rgd, resulted in anti ... | 2011 | 21801372 |
| mhc-dependent ctl repertoire and functional avidity contribute to strain-specific disease susceptibility after murine rsv infection. | susceptibility to respiratory syncytial virus (rsv) infection in mice is genetically determined. while rsv causes little pathology in c57bl/6 mice, pulmonary inflammation and weight loss occur in balb/c mice. using mhc congenic mice, we observed that the h-2(d) allele can partially transfer disease susceptibility to c57bl/6 mice. this was not explained by altered viral elimination or differences in the magnitude of the overall virus-specific ctl response. however, h-2(d) mice showed a more focus ... | 2011 | 21795345 |
| multiple functional domains and complexes of the two nonstructural proteins of human respiratory syncytial virus contribute to interferon suppression and cellular location. | human respiratory syncytial virus (rsv), a major cause of severe respiratory diseases, efficiently suppresses cellular innate immunity, represented by type i interferon (ifn), using its two unique nonstructural proteins, ns1 and ns2. in searching for their mechanism, ns1 was previously shown to decrease levels of traf3 and ikke, whereas ns2 interacted with rig-i and decreased traf3 and stat2. here, we report on the interaction, cellular localization and functional domains of these two proteins. ... | 2011 | 21795342 |
| production of rous sarcoma virus-like particles displaying human transmembrane protein in silkworm larvae and its application to ligand-receptor binding assay. | two types of rous sarcoma virus (rsv) group-antigen protein (gag) virus like particles (vlps), full-length gag (gag701) and rsv protease domain (pr)-deleted mutant (gag577) were expressed in silkworm larvae. gag577 was secreted into hemolymph efficiently using wild type bacmid (wt), cysteine protease-deficient bacmid (cp(-)), cysteine protease and chitinase-deficient bacmid (cp(-)chi(-)) bacmids, but comparatively gag701 secretion levels were low. vlps were purified on 10-60% (v/v) sucrose densi ... | 2011 | 21794838 |
| whole genome characterization of non-tissue culture adapted hrsv strains in severely infected children. | abstract: background: human respiratory syncytial virus (hrsv) is the most important virus causing lower respiratory infection in young children. the complete genetic characterization of rsv clinical strains is a prerequisite for understanding hrsv infection in the clinical context. current information about the genetic structure of the hrsv genome has largely been obtained using tissue culture adapted viruses. during tissue culture adaptation genetic changes can be introduced into the virus gen ... | 2011 | 21794174 |
| genetic dissection of the resistance to rice stripe virus present in the indica rice cultivar 'ir24' | rice stripe disease, caused by rice stripe virus (rsv) and transmitted by the small brown planthopper (laodelphax striatellus fallen), is one of the most serious viral diseases of rice in temperate east asian production regions. prior quantitative trait loci (qtl) mapping has established that oryza sativa l. subsp. indica 'ir24' carries positive alleles at the three loci qstv3, qstv7, and qstv11-i. here, we report an advanced backcross analysis based on three selected chromosome segment substitu ... | 2011 | 21793697 |
| comparison of risk factors between preterm and term infants hospitalized for severe respiratory syncytial virus in the russian federation. | respiratory syncytial virus (rsv) is a leading cause of lower respiratory tract infection in infants. preterm birth, in addition to several demographic and environmental factors, increases the risk for development of severe rsv infection. the purpose of this study was to describe differences in risk factors and protective factors between preterm birth (up to 35 weeks' gestational age) and term infants hospitalized for rsv lower respiratory tract infection in the russian federation during the 200 ... | 2011 | 21792335 |
| effects of decabrominated diphenyl ether (dbde) on developmental immunotoxicity in offspring mice. | decabrominated diphenyl ether (dbde), a representative brominated flame retardant ubiquitous in the environment, is suspected of being hazardous to humans. we evaluated the developmental immunotoxicity of dbde by an assay system using respiratory syncytial virus (rsv) infection in offspring mice. pregnant mice were continuously exposed to dbde (10, 100, 1000, or 10,000ppm) in the diet from gestation day 10 to weaning on postnatal day 21. offspring mice born to these dams were intranasally infect ... | 2008 | 21791381 |
| [detection and clinical characteristics analysis of human bocavirus 1-3 in children for acute respiratory infection in lanzhou area]. | to study the clinical and molecular epidemiology characteristics of human bocavirus 1-3 (hbov1-3) in children for acute respiratory infection in lanzhou area. | 2011 | 21789841 |
| a novel inactivated intranasal respiratory syncytial virus vaccine promotes viral clearance without th2 associated vaccine-enhanced disease. | respiratory syncytial virus (rsv) is a leading cause of bronchiolitis and pneumonia in young children worldwide, and no vaccine is currently available. inactivated rsv vaccines tested in the 1960's led to vaccine-enhanced disease upon viral challenge, which has undermined rsv vaccine development. rsv infection is increasingly being recognized as an important pathogen in the elderly, as well as other individuals with compromised pulmonary immunity. a safe and effective inactivated rsv vaccine wou ... | 2011 | 21789184 |
| a new assay system for evaluation of developmental immunotoxicity of chemical compounds using respiratory syncytial virus infection to offspring mice. | we evaluated the effect of 6-propyl-2-thiouracil (ptu), an anti-thyroid agent, on developmental immunity using respiratory syncytial virus (rsv) infection to offspring mice as a new risk assessment for brominated flame retardants (bfrs), because some bfrs are suspected of affecting the thyroid system. pregnant mice were exposed to ptu in drinking water from gestation day 10 to weaning on postnatal day 21. their offspring mice were infected intranasally with rsv. exposure of 100ppm ptu significan ... | 2008 | 21783838 |
| effect of ozone on susceptibility to respiratory viral infection and virus-induced cytokine secretion. | airway epithelium is the primary target tissue for respiratory viruses as well as an important target of ozone (o(3)) toxicity. a change in the severity of viral airway infection may result from changes in epithelial cell susceptibility to infection, metabolic interference with viral replication, or altered production of immune regulatory molecules by the infected cells as a result of exposure to o(3). in this study we have investigated whether o(3) exposure alters the susceptibility of human ai ... | 1998 | 21781902 |
| increased detection of respiratory viruses in paediatric outpatients with acute respiratory illness by real-time polymerase chain reaction using nasopharyngeal flocked swabs. | detection of respiratory viruses by realtime multiplexed pcr (m-pcr) and of rsv by m-pcr and immunofluorescence(if) was evaluated using specimens collected by nasopharyngeal flocked swab(nfs) and nasal wash(nw). in children with mild respiratory illness, nfs collection was superior to nw collection for detection of viruses by m-pcr (sensitivity 89.6% vs 79.2%, p=0.0043). nfs collection was non-inferior to nw collection in detecting rsv by if. | 2011 | 21775539 |
| the structure and function of the rous sarcoma virus rna stability element. | for simple retroviruses, such as the rous sarcoma virus, post-transcriptional control elements regulate viral rna splicing, export, stability and packaging into virions. these rna sequences interact with cellular host proteins to regulate and facilitate productive viral infections. one such element, known as the rous sarcoma virus stability element (rse), is required for maintaining stability of the full-length unspliced rna. this viral rna serves as the mrna for the gag and pol proteins and als ... | 2011 | 21769913 |
| clara cell protein 10 polymorphism is not associated with severe respiratory syncytial virus infection. | aim: to investigate a possible correlation between severe respiratory syncytial virus (rsv) infection and single-nucleotide polymorphism in the clara cell protein 10 (cc10) gene (a38g). methods: children hospitalized with severe rsv lower respiratory tract (ltri) infection at karolinska university hospital stockholm during three subsequent rsv seasons were selected and genotyped. age-matched controls were used. the two groups were compared regarding single nucleotide polymorphism in the cc10 g ... | 2011 | 21767304 |
| the role of rsv infection in asthma initiation and progression: findings in a mouse model. | respiratory syncytial virus (rsv) is a common cause of severe lower respiratory tract diseases (bronchiolitis and pneumonia) during infancy and early childhood. there is increasing evidence which indicates that severe pulmonary disease caused by rsv infection in infancy is associated with recurrent wheezing and development of asthma later in childhood. however, the underlying mechanisms linking rsv infection to persistent airway hyperresponsiveness and dysfunction are not fully defined. to study ... | 2011 | 21766019 |
| role of the fas/fasl system in a model of rsv infection in mechanically ventilated mice. | infection with respiratory syncytial virus (rsv) in children can progress to respiratory distress and acute lung injury (ali) necessitating mechanical ventilation (mv). mv enhances apoptosis and inflammation in mice infected with pneumonia virus of mice (pvm), a mouse pneumovirus that has been used as a model for severe rsv infection in mice. we hypothesized that the fas/fas ligand (fasl) system, a dual pro-apoptotic/pro-inflammatory system involved in other forms of lung injury, is required for ... | 2011 | 21743025 |
| respiratory syncytial virus binds and undergoes transcription in neutrophils from the blood and airways of infants with severe bronchiolitis. | neutrophils are the predominant cell in the lung inflammatory infiltrate of infants with respiratory syncytial virus (rsv) bronchiolitis. although it has previously been shown that neutrophils from both blood and bronchoalveolar lavage (bal) are activated, little is understood about their role in response to rsv infection. this study investigated whether rsv proteins and mrna are present in neutrophils from blood and bal of infected infants. | 2011 | 21742845 |
| epidemiological study of hospitalization associated with respiratory syncytial virus infection in taiwanese children between 2004 and 2007. | respiratory syncytial virus (rsv) is the leading cause of hospitalization in young children. the population-based burden of rsv hospitalization and the effect of potential risk factors on the severity of illness were evaluated in taiwanese children. | 2011 | 21741007 |
| characterization of human metapneumovirus from pediatric patients with acute respiratory infections in a 4-year period in beijing, china. | human metapneumovirus (hmpv) was discovered by scientists in the netherlands as a novel respiratory virus in 2001 and had been found in children with acute respiratory tract infections (arti) in china. the objective of this study was to determine the importance of hmpv infection in children in beijing and the genotypes of the circulating virus by the surveillance during a four-consecutive-year period. | 2011 | 21740766 |
| new insights into the nuclear localization of retroviral gag proteins. | retroviruses assemble new virus particles that are released by budding from the plasma membranes of infected cells. gag proteins, encoded by retroviruses, orchestrate the assembly of virus particles in close collaboration with host cell machinery. the earliest steps in retrovirus assembly-those immediately following synthesis of gag on cytosolic ribosomes-are poorly understood. rous sarcoma virus (rsv) offers a unique model system for dissecting these early steps because the rsv gag protein unde ... | 2011 | 21738831 |
| paediatric intensive care admissions for respiratory syncytial virus bronchiolitis in france: results of a retrospective survey and evaluation of the validity of a medical information system programme. | summarythe purpose of this study was to describe the characteristics of patients with bronchiolitis admitted to a paediatric intensive care unit (picu), and to evaluate a national registry of hospitalizations (programme de m+®dicalisation des syst+¿mes d'information; pmsi) as a potential source of epidemiological data. of the 49 french picus invited to take part in a retrospective survey of children aged <2 years who were hospitalized during the 2005-2006 epidemic season, 24 agreed to participat ... | 2011 | 21733254 |
| systemic signature of the lung response to respiratory syncytial virus infection. | respiratory syncytial virus is a frequent cause of severe bronchiolitis in children. to improve our understanding of systemic host responses to rsv, we compared balb/c mouse gene expression responses at day 1, 2, and 5 during primary rsv infection in lung, bronchial lymph nodes, and blood. we identified a set of 53 interferon-associated and innate immunity genes that give correlated responses in all three murine tissues. additionally, we identified blood gene signatures that are indicative of ac ... | 2011 | 21731757 |
| p2 of Rice stripe virus (RSV) interacts with OsSGS3 and is a silencing suppressor. | A rice cDNA library was screened by a galactosidase 4 (Gal4)-based yeast two-hybrid system with Rice stripe virus (RSV) p2 as bait. The results revealed that RSV p2 interacted with a rice protein exhibiting a high degree of identity with Arabidopsis thaliana suppressor of gene silencing 3 (AtSGS3). The interaction was confirmed by bimolecular fluorescence complementation assay. SGS3 has been shown to be involved in sense transgene-induced RNA silencing and in the biogenesis of trans-acting small ... | 2011 | 21726383 |
| lack of an exaggerated inflammatory response upon virus infection in cystic fibrosis. | respiratory virus infections play an important role in cystic fibrosis (cf) exacerbations, but underlying pathophysiological mechanisms are poorly understood. we aimed at assessing whether an exaggerated inflammatory response of the airway epithelium upon virus infection could explain the increased susceptibility of cf patients towards respiratory viruses. we used primary bronchial and nasal epithelial cells obtained from healthy control subjects (n=24) and cf patients (n=18). il-6, il-8/cxcl8, ... | 2011 | 21719483 |
| replacement of previously circulating respiratory syncytial virus subtype b strains with the ba genotype in south africa. | respiratory syncytial virus (rsv) is a major cause of bronchiolitis and pneumonia in infants, the immunocompromised, and the elderly in both developed and developing countries. reinfections are common, and g protein variability is one mechanism to overcome herd immunity. this is illustrated by the appearance of the ba genotype with a 60-nucleotide duplication dominating the subtype b genotypes in epidemics worldwide. to investigate the evolution of subtype b in south africa since 2002, the genet ... | 2011 | 21715483 |
| real-world comparison of two molecular methods for detection of respiratory viruses. | abstract: | 2011 | 21714915 |
| il-17-induced pulmonary pathogenesis during respiratory viral infection and exacerbation of allergic disease. | severe respiratory syncytial virus (rsv) infections are characterized by airway epithelial cell damage, mucus hypersecretion, and th2 cytokine production. less is known about the role of il-17. we observed increased il-6 and il-17 levels in tracheal aspirate samples from severely ill infants with rsv infection. in a mouse model of rsv infection, time-dependent increases in pulmonary il-6, il-23, and il-17 expression were observed. neutralization of il-17 during infection and observations from il ... | 2011 | 21703407 |
| Preschool asthma after bronchiolitis in infancy. | Asthma risk is lower after wheezing associated with RSV than with non-RSV infection in infancy. RSV is the main wheezing-associated virus in infants aged <6 months. We evaluated the outcome of children hospitalised for bronchiolitis at <6 months of age, with special focus on viral etiology and early risk factors. Out of 205 infants hospitalised for bronchiolitis at <6 months of age, 127 (62%) attended the control visit at age (mean) 6.5 years, and the parents of additional 39 children were inter ... | 2011 | 21700604 |
| attenuated interleukin-8/leukocyte immunoresponse in preterm infants compared with term infants hospitalized with respiratory syncytial virus bronchiolitis: a pilot study. | decreased transplacental transfer of antibodies and altered immunoresponsiveness may place preterm (pt) infants at higher risk for serious consequences from respiratory syncytial virus (rsv) bronchiolitis. we hypothesize that among infants hospitalized with rsv bronchiolitis, immune response in pt infants may be different when compared with that of term infants. nasal-wash samples were collected from 11 pt (<37 weeks of gestation) and 13 term infants (ôëñ37 weeks of gestation) hospitalized with ... | 2011 | 21683109 |
| increased eosinophilic cationic protein in nasal fluid in hospitalized wheezy infants with rsv infection. | background: respiratory syncytial virus (rsv) is a major respiratory pathogen which causes bronchiolitis with dyspnea and wheezing in children less than 2 years old. rsv bronchiolitis in infancy severe enough to cause hospitalization might be a risk factor for allergic sensitization and bronchial asthma in future. however, the pathophysiology behind this development has not been clearly characterized. to evaluate the existence of airway inflammation and characteristic of rsv bronchiolitis, we an ... | 2011 | 21681019 |
| identification of deletion mutant respiratory syncytial virus strains lacking most of the g protein in immunocompromised children with pneumonia in south africa. | respiratory syncytial virus (rsv) g protein deletion mutants replicate effectively in vitro but have not been detected in nature. subtyping of rsv strains in hospitalized children in south africa identified g protein pcr amplicons significantly reduced in size in 2 out of 209 clinical specimens screened over 4 years. sequence analysis revealed subtype b strains lacking nearly the entire g protein ectodomain in one hiv-positive and one hiv-exposed child hospitalized with pneumonia. the associatio ... | 2011 | 21680500 |
| comparative evaluation of the seegene seeplex rv15 and real-time pcr for respiratory virus detection. | respiratory virus infections contribute substantially both to hospitalizations of young children, and to morbidity in immunocompromised patients such as those with hematological malignancies. their rapid and accurate diagnosis is essential to patient management. to evaluate the prospective utility of seeplex-« dpo technology in respiratory virus diagnosis, a panel of 99 respiratory samples positive by real-time rt-pcr for one or more viruses was assayed by the seegene seeplex-« rv12 system. as w ... | 2011 | 21678451 |
| contribution of common and recently described respiratory viruses to annual hospitalizations in children in south africa. | the contribution of viruses to lower respiratory tract disease in sub-saharan africa where human immunodeficiency virus may exacerbate respiratory infections is not well defined. no data exist on some of these viruses for southern africa. comprehensive molecular screening may define the role of these viruses as single and co-infections in a population with a high hiv-aids burden. to address this, children less than 5 years of age with respiratory infections from 3 public sector hospitals, pretor ... | 2011 | 21678450 |
| wu polyomavirus infection among children in south china. | this study aimed at investigating the prevalence and clinical characteristics of children with respiratory infection by wu polyomavirus (wupyv) in southern china. nasopharyngeal aspirate samples were collected from 771 children with acute respiratory tract infection admitted to hospital and 82 samples from healthy subjects for routine examination at the outpatient service at the second affiliated hospital of shantou university, medical college from july 2008 to june 2009. wupyv was detected by t ... | 2011 | 21678448 |
| antibiotics for bronchiolitis in children. | bronchiolitis is a serious, potentially life-threatening respiratory illness commonly affecting babies. it is often caused by respiratory syncytial virus (rsv). antibiotics are not recommended for bronchiolitis unless there is concern about complications such as secondary bacterial pneumonia or respiratory failure. nevertheless, they are used at rates of 34% to 99% in uncomplicated cases. | 2011 | 21678346 |
| a multi-tiered time-series modelling approach to forecasting respiratory syncytial virus incidence at the local level. | summaryrespiratory syncytial virus (rsv) is the most common cause of documented viral respiratory infections, and the leading cause of hospitalization, in young children. we performed a retrospective time-series analysis of all patients aged <18 years with laboratory-confirmed rsv within a network of multiple affiliated academic medical institutions. forecasting models of weekly rsv incidence for the local community, inpatient paediatric hospital and paediatric intensive-care unit (picu) were cr ... | 2011 | 21676348 |
| epidemiological and clinical features of respiratory viral infections in hospitalized children during the circulation of influenza virus a(h1n1) 2009. | please cite this paper as: zuccotti et┬áal. (2011) epidemiological and clinical features of respiratory viral infections in hospitalized children during the circulation of influenza virus a(h1n1) 2009. influenza and other respiratory viruses. doi: 10.1111/j.1750-2659.2011.00263.x. backgroundôçé seasonal influenza viruses and respiratory syncytial virus (rsv) are primary causes of acute respiratory tract infections (artis) in children. new respiratory viruses including human metapneumovirus (hmpv ... | 2011 | 21668662 |
| multiple versus single virus respiratory infections: viral load and clinical disease severity in hospitalized children. | please cite this paper as: martin et┬áal. (2011) multiple versus single virus respiratory infections: viral load and clinical disease severity in hospitalized children. influenza and other respiratory viruses. doi: 10.1111/j.1750-2659.2011.00265.x00 (00), 000-000. backgroundôçé molecular testing for viral pathogens has resulted in increasing detection of multiple viruses in respiratory secretions of ill children. the clinical impact of multiple virus infections on clinical presentation and outco ... | 2011 | 21668660 |
| economic evaluation of palivizumab in children with congenital heart disease: a canadian perspective. | respiratory syncytial virus (rsv) is a common cause of bronchiolitis in infants. in children with congenital heart disease (chd), it is associated with significant morbidity and mortality. palivizumab is a monoclonal antibody that reduces the number of rsv-associated hospitalizations in children with chd. we sought to assess cost savings and cost-effectiveness of palivizumab in children < 2 years old with hemodynamically significant chd in a provincially administered rsv prophylaxis program. | 2011 | 21664100 |
| a molecular epidemiological study of respiratory viruses detected in japanese children with acute wheezing illness. | recent studies strongly suggest that some respiratory viruses are associated with the induction of acute wheezing and/or exacerbation of bronchial asthma. however, molecular epidemiology of these viruses is not exactly known. | 2011 | 21663657 |
| prevalence of respiratory syncytial virus infection among hospitalized children presenting with acute lower respiratory tract infections. | objective: to evaluate the prevalence of rsv among hospitalized young children presenting with alri in bangalore, india. methods: respiratory syncytial virus (rsv) antigen detection was performed by direct fluorescent antibody (dfa) staining on 77 nasopharyngeal wash samples collected from hospitalized children below 2 years of age with a diagnosis of acute lower respiratory tract infection (alri). results: out of 77 samples tested for rsv with dfa, 17 (22.1%) were found rsv-positive with a mea ... | 2011 | 21660398 |