Publications

[studies on echo 9 virus. ii. biological properties of the "cold" mutant].		1968	4969559
change in chang liver cells from virus susceptibility to resistance mediated by persistent infection with echovirus type 9.		1966	4960050
glomerulonephritis induced in mice by echo 9 virus.		1968	4881780
severe neonatal illness associated with echo 9 virus infection.		1973	4700731
epidemic of echovirus type 9 infection. certain clinical and epidemiologic features.		1973	4510097
acute ascending polyradiculomyelitis. associated with echo 9 virus.		1967	4292732
use of win 51711 to prevent echovirus type 9-induced paralysis in suckling mice.	the systemic efficacy of the antipicornavirus agent win 51711 was assessed in suckling mice infected with echovirus type 9 (barty strain). single, daily intraperitoneal doses of win 51711 as low as 10 mg/kg significantly (p less than .01) slowed the rate of onset of echovirus-induced paralysis and reduced the number of paralyzed animals. intraperitoneal administration beginning 2 hr before infection or 48 hr after infection was equally effective in preventing paralysis. oral administration of wi ...	1986	3745975
biochemistry and pathogenicity of echovirus 9. ii. mutants of echovirus 9, strain hill, with altered capsid surface properties.	the two echovirus 9 strains hill and barty have been shown previously not only to differ in pathogenicity for newborn mice but also in a number of in vitro characteristics which depend on viral capsid structure. three spontaneously occurring mutants of the mouse-apathogenic echovirus 9 prototype strain hill being resistant to an inhibitor of plaque formation present in agar were isolated and compared biochemically and biophysically to their parent strain and to the mouse-pathogenic echovirus 9 s ...	1986	3739228
molecular cloning and sequence determination of the genomic regions encoding protease and genome-linked protein of three picornaviruses.	to investigate the degree of similarity between picornavirus proteases, we cloned the genomic cdnas of an enterovirus, echovirus 9 (strain barty), and two rhinoviruses, serotypes 1a and 14lp, and determined the nucleotide sequence of the region which, by analogy to poliovirus, encodes the protease. the nucleotide sequence of the region encoding the genome-linked protein vpg, immediately adjacent to the protease, was also determined. comparison of nucleotide and deduced amino acid sequences with ...	1986	3512851
koplik spots and echo 9 virus.		1987	3481055
design and construction of a versatile system for the expression of foreign genes in plants.	we have built a series of vectors to allow the constitutive or light-regulated expression of foreign genes in plants. these vectors carry expression cassettes consisting of either the cauliflower mosaic virus 35s promoter or the pea rbcs-e9 promoter, a multiple cloning site derived from m13um20, and the rbcs-e9 polyadenylation site. these cassettes have been incorporated into pbr322-based or rk2-based replicons to facilitate direct dna uptake or agrobacterium tumefaciens-mediated gene transfer. ...	1987	3443303
morphological changes and membrane potential of human granulocytes under influence of chemotactic peptide and/or echo-virus, type 9.	the membrane potential, em, of human granulocytes (pmns), was recorded using glass microelectrodes. the membrane potential em exhibited potential fluctuations accompanied by characteristic changes of cell shape. the periodic potential fluctuations (7-s, 70-s, and 260-s periodicities) ascertained by the autocorrelation technique, suggested the existence of an internal clock. the chemoattractant f-met-leu-phe (fmlp) had no influence on the periodicities, whereas the amplitudes of the fluctuations ...	1988	3398429
development and application of rna probes for the study of picornaviruses.	single-stranded rna probes were developed from cloned cdna fragments derived from four picornaviruses; poliovirus type 1, coxsackievirus b3, echovirus 9 and theiler's murine encephalomyelitis virus. in comparative testing with a nick-translated cdna probe, the rna probe was found to be 10-100-fold more sensitive. hybridization conditions were optimized for rna probes to picornavirus targets. longer hybridization times gave an improved signal, as did a temperature of 50 degrees c. formamide conce ...	1988	3380106
biochemistry and pathogenicity of echovirus 9. iii. thermosensitive mutants of echovirus 9, strain barty, with reduced pathogenicity for newborn mice.	different clinical isolates of echovirus 9 are known to vary strikingly with regard to pathogenicity. prototype strain hill and strain barty have previously been shown to differ not only in paralytogenic potency for newborn mice but also in a number of in vitro characteristics related to virus capsid structures. a series of mutants of strain barty, thermosensitive for replication at 40 degrees c, was isolated after mutagenization with 5-fluorouracil. for all mutants the virus dose required to pa ...	1988	3362095
[[(4,5-dihydro-2-oxazolyl)phenoxy]alkyl]isoxazoles. inhibitors of picornavirus uncoating.	a series of [[(4,5-dihydro-2-oxazolyl)phenoxy]alkyl]isoxazoles has been synthesized and evaluated as antipicornavirus agents. the effect of alkyl groups in the 4- and 5-position of the oxazoline ring, as well as the alkyl chain length, on antiviral activity was examined. compound 14 was evaluated in vivo and was found to significantly reduce mortality at an oral dose of 4 mg/kg in mice infected intracerebrally with poliovirus-2. compound 14 was also effective in preventing paralysis when adminis ...	1985	2999407
mutational analysis of the genome-linked protein vpg of poliovirus.	using a mutagenesis cartridge (r. j. kuhn, h. tada, m. f. ypma-wong, j. j. dunn, b. l. semler, and e. wimmer, proc. natl. acad. sci. usa 85:519-523, 1988), we have generated single and multiple amino acid replacement mutants, as well as a single amino acid insertion mutant in the genome-linked protein vpg of poliovirus. moreover, we constructed three different 5-amino-acid insertion mutants that map close to the c terminus of 3a, a viral polypeptide whose coding sequence is adjacent to vpg. tran ...	1988	2845132
seed-transmissible expression of mammalian metallothionein in transgenic tobacco.	a binary plasmid was constructed to contain the mouse metallothionein c-dna, the constitutive 35s promoter from cauliflower mosaic virus, the polyadenylation signal from the pea rbcs-e9 gene and several selectable markers. the plasmid was transferred to agrobacterium tumefaciens and the leaf disc method was used to transform tobacco. callus and shoots were regenerated in the presence of kanamycin and transformed plants were obtained. southern, northern and western blot analysis demonstrated inte ...	1988	2829879
vinyl versus latex gloves as barriers to transmission of viruses in the health care setting.	one type of vinyl and seven types of latex gloves without visual defects were tested with respect to their barrier function against high concentrations of three viruses of varying size: herpes simplex virus type 1 (hsv-1, 180 nm), human immunodeficiency virus type 1 (hiv-1, 100 nm), and echovirus type 9 (echo 9, 25 nm). viral suspensions of hsv-1 (10(8) tcd50/ml), hiv-1 (10(5) tcd50/ml), and echovirus type 9 (10(7.5)tcd 50/ml) were placed in an inverted glove finger immersed in media and maintai ...	1989	2703958
acute renal failure due to rhabdomyolysis associated with echovirus 9 infection: a case report and review of literature.	the occurrence of rhabdomyolysis and acute renal failure associated with viral infections is uncommon. we report a case of a 17-year-old female suffering from rhabdomyolysis caused by echovirus 9 in which the patient recovered after being treated for acute renal failure with hemodialysis. among 42 cases of virus-induced rhabdomyolysis review in japanese and english literatures, influenza virus is the most common cause of rhabdomyolysis, coxsackie virus tends to be next most common cause. renal f ...	1989	2659856
in vitro and in vivo activities of win 54954, a new broad-spectrum antipicornavirus drug.	win 54954 (5-[5-[2,6-dichloro-4-(4,5-dihydro-2-oxazolyl)phenoxy]pentyl]-3- methylisoxazole) is a new member of the class of broad-spectrum antipicornavirus compounds known to bind in a hydrophobic pocket within virion capsid protein vp1. in plaque reduction assays, win 54954 reduced plaque formation of 50 of 52 rhinovirus serotypes (mics ranged from 0.007 to 2.2 micrograms/ml). a concentration of 0.28 microgram/ml was effective in inhibiting 80% of the 52 serotypes tested (ec80). win 54954 was a ...	1989	2559655
acid stability of hepatitis a virus.	the acid stability of unpurified and highly purified hepatitis a virus (hav) was tested and compared with that of poliovirus type 1, coxsackievirus types a9 and b1 and echovirus type 9. only hav had a high residual infectivity after 2 h of exposure to ph 1 at room temperature, remaining infectious for up to 5 h. at 38 degrees c, ph 1, hav remained infectious for 90 min. highly purified hav was found to be infectious for 8 h at ph 1 and room temperature. this indicates that the increased stabilit ...	1989	2550575
clinical observations and virological study of aseptic meningitis in the kaohsiung area.	during 1988, an endemic outbreak of aseptic meningitis was noted in the kaohsiung area. throughout the year, a total of 89 cases were identified by cerebrospinal fluid (csf) examination at the pediatric department of kaohsiung medical college. the peak incidence was from june to october. scattered cases still occurred during november and december. the male to female ratio was 1.7:1 and the age distribution ranged from 1 month to 15 years old. two peaks of age distribution were observed; one in i ...	1990	1981774
several distinct types of sequence elements are required for efficient mrna 3' end formation in a pea rbcs gene.	we have conducted an extensive linker substitution analysis of the polyadenylation signal from a pea rbcs gene. from these studies, we can identify at least two, and perhaps three, distinct classes of cis element involved in mrna 3' end formation in this gene. one of these, termed the far-upstream element, is located between 60 and 120 nt upstream from its associated polyadenylation sites and appears to be largely composed of a series of ug motifs. a second, termed the near-upstream element, is ...	1992	1448074
[echo-9 virus infections in the records of the provincial hospital for infectious diseases of the mecidal academy in gdańsk].		1975	1178566
echo 9 infection in pregnant women with suspected rubella.	both rubella virus and echovirus 9 (echo 9) were epidemic in scotland during the summer of 1973; both viruses can cause a mild febrile illness with rash. sera from 286 rubella-negative pregnant women were tested for neutralizing antibodies to echo 9 virus; 40 women had antibody titres suggestive of recent infection. prospective studies on the outcome of these pregnancies are in progress but preliminary results suggest no connexion between fetal damage and echo 9 infection.	1975	1127119
fatal neonatal pneumonia caused by echovirus type 9.		1977	910771
echovirus-associated polyarthritis. report of a case with synovial fluid and synovial histologic characterization.	a patient with fever, myalgias, and acute polyarthritis in whom echovirus 9 was isolated from throat and rectal swab specimens is described. all symptoms resolved spontaneously over a one week period except for the arthritis which required three months for complete recovery. a mildly inflammatory synovial fluid and histologic picture were observed. attempts to isolate the virus from synovial fluid and synovium during acute illness were unsuccessful.	1978	737022
[b and t lymphocytes in peripheral blood of children with infections caused by echovirus 9].		1977	304572
a study of infections and illnesses in a day nursery based on inclusion-bearing cells in the urine and infectious agent in faeces, urine and nasal secretion.	during an epidemic-free period of nearly 2 years infections and illnesses were followed in a group of small children (2-3 1/2 years) in a day nursery. the incidence of illness was fairly low, 6.3 per child and year. the viral infections were followed by means of the inclusion reaction in the urine and isolation of virus, and the bacterial by culture of nasal secretion. in 60% of the cases the viral infection was accompanied by illness. in 57 cases of illness there were 27 (47%) with merely virus ...	1979	231298
nuclear abnormalities in muscle regenerating after echo 9 virus infection in mice.		1978	203663
antiviral activity of a pyrazino-pyrazine derivative.	the 2,3-dihydroxy-6-bromo-pyrazino-[2,3-beta]-pyrazine is a substance selected during the antiviral screening of pyrazino-pyrazine derivatives. the compound shows antiviral activity in vitro against measles, ndv, some influenza viruses and against herpes simplex and zoster, infectious canine hepatitis and vaccinia viruses. it had no effect on echo 9 virus. therapeutic trials showed activity also on herpetic keratoconjunctivitis experimentally induced in rabbits.	1975	169110