Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| rna virus reverse genetics and vaccine design. | rna viruses are capable of rapid spread and severe or potentially lethal disease in both animals and humans. the development of reverse genetics systems for manipulation and study of rna virus genomes has provided platforms for designing and optimizing viral mutants for vaccine development. here, we review the impact of rna virus reverse genetics systems on past and current efforts to design effective and safe viral therapeutics and vaccines. | 2014 | 24967693 |
| production of a neutralizing antibody against envelope protein of dengue virus type 2 using the linear array epitope technique. | dengue virus (denv; genus flavivirus) contains a positive-stranded rna genome. binding of denv to host cells is mediated through domain iii of the viral envelope protein. many therapeutic mabs against domain iii have been generated and characterized because of its high antigenicity. we have previously established a novel pcr method named the linear array epitope (lae) technique for producing monoclone-like polyclonal antibodies. to prove this method could be utilized to produce antibody against ... | 2014 | 24948392 |
| substitution of ns5 n-terminal domain of dengue virus type 2 rna with type 4 domain caused impaired replication and emergence of adaptive mutants with enhanced fitness. | flavivirus ns3 and ns5 are required in viral replication and 5'-capping. ns3 has ns2b-dependent protease, rna helicase, and 5'-rna triphosphatase activities. ns5 has 5'-rna methyltransferase (mt)/guanylyltransferase (gt) activities within the n-terminal 270 amino acids and the rna-dependent rna polymerase (pol) activity within amino acids 271-900. a chimeric ns5 containing the d4mt/d4gt and the d2pol domains in the context of wild-type (wt) d2 rna was constructed. rnas synthesized in vitro were ... | 2014 | 24904061 |
| spatiotemporal dynamics of denv-2 asian-american genotype lineages in the americas. | the asian/american (as/am) genotype of dengue virus type 2 (denv-2) has been evolving in the americas over the last 30 years, leading to several waves of dengue epidemics and to the emergence of different viral lineages in the region. in this study, we investigate the spatiotemporal dissemination pattern of the denv-2 lineages at a regional level. we applied phylogenetic and phylogeographic analytical methods to a comprehensive data set of 582 denv-2 e gene sequences of the as/am genotype isolat ... | 2014 | 24897118 |
| molecular determinants of dengue virus 2 envelope protein important for virus entry in fcγriia-mediated antibody-dependent enhancement of infection. | antibody-dependent enhancement (ade) of infection may cause severe illness in patients suffering a secondary infection by a heterologous dengue virus (denv) serotype. during ade of infection, cross-reactive non- or poorly-neutralizing antibodies form infectious virus-ab complexes with the newly infecting serotype and enhance virus infection by binding to the fcγ receptors (fcγr) on fcγr-bearing cells. in this study, we determined that molecular determinants of denv2 envelope protein critical for ... | 2014 | 24889243 |
| capsid protein: evidences about the partial protective role of neutralizing antibody-independent immunity against dengue in monkeys. | the role of cellular immune response in dengue virus infection is not yet fully understood. only few studies in murine models propose that cd8(+) t-cells are associated with protection from infection and disease. at the light of recent reports about the protective role of cd8(+) t-cells in humans and the no correlation between neutralizing antibodies and protection observed in several studies, a vaccine based on cell-mediated immunity constitute an attractive approach. our group has developed a ... | 2014 | 24889226 |
| infection with dengue-2 virus alters proteins in naturally expectorated saliva of aedes aegypti mosquitoes. | dengue virus (denv) is responsible for up to approximately 300 million infections and an increasing number of deaths related to severe manifestations each year in affected countries throughout the tropics. it is critical to understand the drivers of this emergence, including the role of vector-virus interactions. when a denv-infected aedes aegypti mosquito bites a vertebrate, the virus is deposited along with a complex mixture of salivary proteins. however, the influence of a denv infection upon ... | 2014 | 24886023 |
| safety and immunogenicity of a rederived, live-attenuated dengue virus vaccine in healthy adults living in thailand: a randomized trial. | safety and immunogenicity of two formulations of a live-attenuated tetravalent dengue virus (tden) vaccine produced using rederived master seeds from a precursor vaccine were tested against a placebo control in a phase ii, randomized, double blind trial (nct00370682). two doses were administered 6 months apart to 120 healthy, predominantly flavivirus-primed adults (87.5% and 97.5% in the two vaccine groups and 92.5% in the placebo group). symptoms and signs reported after vaccination were mild t ... | 2014 | 24865677 |
| recombinant dengue 2 virus ns3 protein conserves structural antigenic and immunological properties relevant for dengue vaccine design. | the ns3 protein is a multifunctional non-structural protein of flaviviruses implicated in the polyprotein processing. the predominance of cytotoxic t cell lymphocytes epitopes on the ns3 protein suggests a protective role of this protein in limiting virus replication. in this work, we studied the antigenicity and immunogenicity of a recombinant ns3 protein of the dengue virus 2. the full-length ns3 gene was cloned and expressed as a his-tagged fusion protein in escherichia coli. the pns3 protein ... | 2014 | 24854144 |
| a "microrna-like" small rna expressed by dengue virus? | 2014 | 24853506 | |
| a tetravalent dengue vaccine containing a mix of domain iii-p64k and domain iii-capsid proteins induces a protective response in mice. | recombinant fusion proteins containing domain iii of the dengue virus envelope protein fused to the p64k protein from neisseria meningitidis and domain iii of dengue virus type 2 (d2) fused to the capsid protein of this serotype were immunogenic and conferred protection in mice against lethal challenge, as reported previously. combining the domain iii-p64k recombinant proteins of dengue virus types 1, 3 and 4 (d1, d3, and d4) with the domain iii-capsid protein from d2, we obtained a novel tetrav ... | 2014 | 24841761 |
| fitness impact and stability of a transgene conferring resistance to dengue-2 virus following introgression into a genetically diverse aedes aegypti strain. | in 2006, we reported a mariner (mos1)-transformed aedes aegypti line, carb77, which was highly resistant to dengue-2 virus (denv2). carb77 mosquitoes expressed a denv2-specific inverted-repeat (ir) rna in midgut epithelial cells after ingesting an infectious bloodmeal. the ir-rna formed double-stranded denv2-derived rna, initiating an intracellular antiviral rna interference (rnai) response. however, carb77 mosquitoes stopped expressing the ir-rna after 17 generations in culture and lost their d ... | 2014 | 24810399 |
| chloroquine interferes with dengue-2 virus replication in u937 cells. | the objective of this study was to investigate the use of chloroquine (clq) as an antiviral agent against dengue. chloroquine, an amine acidotropic drug known to affect intracellular exocytic pathways by increasing endosomal ph, was used in the in vitro treatment of u937 cells infected with dengue virus type 2 (denv-2). viral replication was assessed by quantification of virus produced through detection of copy numbers of denv-2 rna, plaque assay and indirect immunofluorescence. qrt-pcr and plaq ... | 2014 | 24773578 |
| interferon-mediated isg15 conjugation restricts dengue virus 2 replication. | isgylation, an ubiquitin-like post-translational modification by isg15, has been reported to participate in the interferon (ifn)-mediated antiviral response. in this study, we analyzed the functional role of isgylation in dengue virus 2 (denv-2) replication. overexpression of isg15 was found to significantly suppress the amount of extracellular infectious virus released, while intracellular viral rna was unaffected. this effect was not observed with a conjugation-defective isg15 mutant. in addit ... | 2014 | 24769207 |
| acute undifferentiated febrile illness in rural cambodia: a 3-year prospective observational study. | in the past decade, malaria control has been successfully implemented in cambodia, leading to a substantial decrease in reported cases. wide-spread use of malaria rapid diagnostic tests (rdts) has revealed a large burden of malaria-negative fever cases, for which no clinical management guidelines exist at peripheral level health facilities. as a first step towards developing such guidelines, a 3-year cross-sectional prospective observational study was designed to investigate the causes of acute ... | 2014 | 24755844 |
| development of vhh antibodies against dengue virus type 2 ns1 and comparison with monoclonal antibodies for use in immunological diagnosis. | the possibility of using variable domain heavy-chain antibodies (vhh antibodies) as diagnostic tools for dengue virus (denv) type 2 ns1 protein was investigated and compared with the use of conventional monoclonal antibodies. after successful expression of denv type 2 ns1 protein, the genes of vhh antibodies against ns1 protein were biopanned from a non-immune llama library by phage display. vhh antibodies were then expressed and purified from escherichia coli. simultaneously, monoclonal antibod ... | 2014 | 24751715 |
| model for vaccine design by prediction of b-epitopes of iedb given perturbations in peptide sequence, in vivo process, experimental techniques, and source or host organisms. | perturbation methods add variation terms to a known experimental solution of one problem to approach a solution for a related problem without known exact solution. one problem of this type in immunology is the prediction of the possible action of epitope of one peptide after a perturbation or variation in the structure of a known peptide and/or other boundary conditions (host organism, biological process, and experimental assay). however, to the best of our knowledge, there are no reports of gen ... | 2014 | 24741624 |
| the relationship between the structure of the tick-borne encephalitis virus strains and their pathogenic properties. | tick-borne encephalitis virus (tbev) is transmitted to vertebrates by taiga or forest ticks through bites, inducing disease of variable severity. the reasons underlying these differences in the severity of the disease are unknown. in order to identify genetic factors affecting the pathogenicity of virus strains, we have sequenced and compared the complete genomes of 34 far-eastern subtype (fe) tbev strains isolated from patients with different disease severity (primorye, the russian far east). w ... | 2014 | 24740396 |
| evaluation of dengue antiviral candidates in vivo in mouse model. | in vivo evaluation of antiviral compounds can serve as criteria in the drug discovery process for selection of compounds that are suitable to enter late preclinical studies and further development. dengue virus serotypes 1-4 can infect and replicate in the interferon type i and type ii receptor deficient mice (ag129). here we describe the use of a mouse-adapted dengue 2 virus strain (s221) that has been used to develop a robust lethal model of infection. treatment with small molecule inhibitors ... | 2014 | 24696350 |
| construction of plasmid, bacterial expression, purification, and assay of dengue virus type 2 ns5 methyltransferase. | dengue virus (denv), a member of mosquito-borne flavivirus, causes self-limiting dengue fever as well as life-threatening dengue hemorrhagic fever and dengue shock syndrome. its positive sense rna genome has a cap at the 5'-end and no poly(a) tail at the 3'-end. the viral rna encodes a single polyprotein, c-prm-e-ns1-ns2a-ns2b-ns3-ns4a-ns4b-ns5. the polyprotein is processed into 3 structural proteins (c, prm, and e) and 7 nonstructural (ns) proteins (ns1, ns2a, ns2b, ns3, ns4a, ns4b, ns5). ns3 a ... | 2014 | 24696348 |
| targeted mutagenesis of dengue virus type 2 replicon rna by yeast in vivo recombination. | the use of cdna infectious clones or subgenomic replicons is indispensable in studying flavivirus biology. mutating nucleotides or amino acid residues gives important clues to their function in the viral life cycle. however, a major challenge to the establishment of a reverse genetics system for flaviviruses is the instability of their nucleotide sequences in escherichia coli. thus, direct cloning using conventional restriction enzyme-based procedures usually leads to unwanted rearrangements of ... | 2014 | 24696336 |
| rho'ing in and out of cells: viral interactions with rho gtpase signaling. | rho gtpases are key regulators of actin and microtubule dynamics and organization. increasing evidence shows that many viruses have evolved diverse interactions with rho gtpase signaling and manipulate them for their own benefit. in this review, we discuss how rho gtpase signaling interferes with many steps in the viral replication cycle, especially entry, replication, and spread. seen the diversity between viruses, it is not surprising that there is considerable variability in viral interaction ... | 2014 | 24691164 |
| dengue transmission model by means of viremic adult immuno-competent mouse. | dengue virus infection manifests in three distinct forms in humans: dengue fever, dengue hemorrhagic fever, and dengue shock syndrome. infection with the virus is a fatal disease; no vaccine is available and prevention depends on interruption of the chain of transmission. the study of dengue viral transmission by mosquitoes is hindered due to the lack of an affordable animal model. in general, immuno-competent mice are used as a simple and inexpensive animal model, but mice are not susceptible t ... | 2014 | 24685121 |
| amodiaquine, an antimalarial drug, inhibits dengue virus type 2 replication and infectivity. | dengue virus serotypes 1-4 (denv1-4) are transmitted by mosquitoes which cause most frequent arboviral infections in the world resulting in ∼390 million cases with ∼25,000 deaths annually. there is no vaccine or antiviral drug currently available for human use. compounds containing quinoline scaffold were shown to inhibit flavivirus ns2b-ns3 protease (ns2b-ns3pro) with good potencies. in this study, we screened quinoline derivatives, which are known antimalarial drugs for inhibition of denv2 and ... | 2014 | 24680954 |
| high-throughput quantitative proteomic analysis of dengue virus type 2 infected a549 cells. | disease caused by dengue virus is a global health concern with up to 390 million individuals infected annually worldwide. there are no vaccines or antiviral compounds available to either prevent or treat dengue disease which may be fatal. to increase our understanding of the interaction of dengue virus with the host cell, we analyzed changes in the proteome of human a549 cells in response to dengue virus type 2 infection using stable isotope labelling in cell culture (silac) in combination with ... | 2014 | 24671231 |
| parallel solution-phase synthesis and general biological activity of a uridine antibiotic analog library. | a small library of ninety four uridine antibiotic analogs was synthesized, under the pilot scale library (psl) program of the nih roadmap initiative, from amine 2 and carboxylic acids 33 and 77 in solution-phase fashion. diverse aldehyde, sulfonyl chloride, and carboxylic acid reactant sets were condensed to 2, leading after acid-mediated hydrolysis, to the targeted compounds 3-32 in good yields and high purity. similarly, treatment of 33 with diverse amines and sulfonamides gave 34-75. the coup ... | 2014 | 24661222 |
| vertical transmission of dengue virus in aedes aegypti collected in puerto iguazú, misiones, argentina. | a finding of vertical transmission of the den 3 virus in male specimens of aedes aegypti, collected in the 2009 fall-winter period, in puerto iguazú city, misiones, argentina, using the rt-pcr technique in a 15-specimen pool is reported. this result is analyzed within the context of the epidemiological situation of argentina's northeast border. | 0 | 24626420 |
| dengue virus type 2 (denv2)-induced oxidative responses in monocytes from glucose-6-phosphate dehydrogenase (g6pd)-deficient and g6pd normal subjects. | dengue virus is endemic in peninsular malaysia. the clinical manifestations vary depending on the incubation period of the virus as well as the immunity of the patients. glucose-6-phosphate dehydrogenase (g6pd) deficiency is prevalent in malaysia where the incidence is 3.2%. it has been noted that some g6pd-deficient individuals suffer from more severe clinical presentation of dengue infection. in this study, we aim to investigate the oxidative responses of denv2-infected monocytes from g6pd-def ... | 2014 | 24625456 |
| genetically engineered synthetic miniaturized versions of plasmodium falciparum uvrd helicase are catalytically active. | helicases catalyze unwinding of double stranded nucleic acids in an energy-dependent manner. we have reported characterization of uvrd helicase from plasmodium falciparum. we reported that the n-terminal and c-terminal fragments of pfuvrd contain characteristic atpase and dna helicase activities. here we report the generation and characterization of a genetically engineered version of pfuvrd and its derivatives. this synthetic uvrd (sud) contains all the conserved domains of pfuvrd but only the ... | 2014 | 24608129 |
| changes in antiviral susceptibility to entry inhibitors and endocytic uptake of dengue-2 virus serially passaged in vero or c6/36 cells. | the aim of the present study was to analyze the influence of virus origin, mammalian or mosquito cell-derived, on antiviral susceptibility of denv-2 to entry inhibitors and the association of this effect with any alteration in the mode of entry into the cell. to this end, ten serial passages of denv-2 were performed in mosquito c6/36 cells or monkey vero cells and the antiviral susceptibility of each virus passage to sulfated polysaccharides (sps), like heparin and carrageenans, was evaluated by ... | 2014 | 24583230 |
| serological evidence of widespread circulation of west nile virus and other flaviviruses in equines of the pantanal, brazil. | a recent study reported neutralizing antibodies to west nile virus (wnv) in horses from four ranches of southern pantanal. to extend that study, a serosurvey for wnv and 11 brazilian flaviviruses was conducted with 760 equines, 238 sheep and 61 caimans from 17 local cattle ranches. among the tested equines, 32 were collected from a ranch where a neurologic disorder outbreak had been recently reported. the sera were initially screened by using a blocking elisa and then titrated by 90% plaque-redu ... | 2014 | 24551266 |
| microrna-like viral small rna from dengue virus 2 autoregulates its replication in mosquito cells. | micrornas (mirnas) are small regulatory rnas that play significant roles in most cellular processes. in the seemingly endless arms race between hosts and pathogens, viruses also encode mirnas that facilitate successful infection. in search of functional mirnas or viral small rnas (vsrnas) encoded by dengue virus (denv), deep sequencing data of virus-infected aedes aegypti mosquitoes were used. from six vsrnas, with candidate stem-loop structures in the 5' and 3' untranslated regions of the viral ... | 2014 | 24550303 |
| dengue virus serotype 1 non-structural protein ns5 expression interferes with hiv replication in a cd4+ t-cell line. | 2014 | 24470566 | |
| a potential role of myeloid dap12-associating lectin (mdl)-1 in the regulation of inflammation in rheumatoid arthritis patients. | the pathogenic roles of myeloid dap12-associating lectin-1(mdl-1) and dap12 in human rheumatoid arthritis (ra) remain unknown. frequencies of mdl-1-expressing monocytes in 22 active ra patients, 16 inactive ra patients, 12 osteoarthritis (oa) patients and 10 healthy controls (hc) were determined by flow-cytometry analysis. the mrna expression levels of mdl-1 and dap12 on pbmcs were evaluated by quantitative pcr, and their protein expression levels in the synovium were examined by immunohistochem ... | 2014 | 24465901 |
| genesv - an approach to help characterize possible variations in genomic and protein sequences. | a computational approach for identification and assessment of genomic sequence variability (genesv) is described. for a given nucleotide sequence, genesv collects information about the permissible nucleotide variability (changes that potentially preserve function) observed in corresponding regions in genomic sequences, and combines it with conservation/variability results from protein sequence and structure-based analyses of evaluated protein coding regions. genesv was used to predict effects (f ... | 2014 | 24453480 |
| effect of dengue-2 virus infection on protein expression in the salivary glands of aedes aegypti mosquitoes. | dengue virus (denv) is the most important mosquito-transmitted flavivirus that is transmitted throughout the tropical and subtropical regions of the world. the primary mosquito vector of denv in urban locations is aedes aegypti. key to understanding the transmission of denv is the relationship between pathogen and vector. accordingly, we report our preliminary characterization of the differentially expressed proteins from ae. aegypti mosquitoes after denv infection. we investigated the virus-vec ... | 2014 | 24445208 |
| inhibition of hepatitis c virus replication by gs-6620, a potent c-nucleoside monophosphate prodrug. | as a class, nucleotide inhibitors (nis) of the hepatitis c virus (hcv) nonstructural protein 5b (ns5b) rna-dependent rna polymerase offer advantages over other direct-acting antivirals, including properties, such as pangenotype activity, a high barrier to resistance, and reduced potential for drug-drug interactions. we studied the in vitro pharmacology of a novel c-nucleoside adenosine analog monophosphate prodrug, gs-6620. it was found to be a potent and selective hcv inhibitor against hcv repl ... | 2014 | 24419349 |
| genome-wide rnai screen identifies novel host proteins required for alphavirus entry. | the enveloped alphaviruses include important and emerging human pathogens such as chikungunya virus and eastern equine encephalitis virus. alphaviruses enter cells by clathrin-mediated endocytosis, and exit by budding from the plasma membrane. while there has been considerable progress in defining the structure and function of the viral proteins, relatively little is known about the host factors involved in alphavirus infection. we used a genome-wide sirna screen to identify host factors that pr ... | 2013 | 24367265 |
| light-addressable measurements of cellular oxygen consumption rates in microwell arrays based on phase-based phosphorescence lifetime detection. | a digital light modulation system that utilizes a modified commercial digital micromirror device (dmd) projector, which is equipped with a uv light-emitting diode as a light modulation source, has been developed to spatially direct excited light toward a microwell array device to detect the oxygen consumption rate (ocr) of single cells via phase-based phosphorescence lifetime detection. the microwell array device is composed of a combination of two components: an array of glass microwells contai ... | 2012 | 24348889 |
| development of a novel dna-launched dengue virus type 2 infectious clone assembled in a bacterial artificial chromosome. | major progress in dengue virus (denv) biology has resulted from the use of infectious clones obtained through reverse genetics. the construction of these clones is commonly based on high- or low-copy number plasmids, yeast artificial chromosomes, yeast-escherichia coli shuttle vectors, and bacterial artificial chromosomes (bacs). prokaryotic promoters have consistently been used for the transcription of these clones. the goal of this study was to develop a novel denv infectious clone in a bac un ... | 2014 | 24342140 |
| dengue virus subgenomic rna induces apoptosis through the bcl-2-mediated pi3k/akt signaling pathway. | we report a rna species of 429 nucleotides derived from the 3' untranslated region of the viral genome in dengue 2 virus (denv-2) infected cells. the 3' terminal of viral rna contained specific conserved structures that are important for the production of subgenomic rna. transient replicon assays suggested that loss of this small rna has little effect on viral replication, and genetic analysis using recombinant viruses demonstrated that the existence of this subgenomic rna is not essential for t ... | 2014 | 24314632 |
| flavonoid from carica papaya inhibits ns2b-ns3 protease and prevents dengue 2 viral assembly. | dengue virus belongs to the virus family flaviviridae. dengue hemorrhagic disease caused by dengue virus is a public health problem worldwide. the viral non structural 2b and 3 (ns2b-ns3) protease complex is crucial for virus replication and hence, it is considered to be a good anti-viral target. leaf extracts from carica papaya is generally prescribed for patients with dengue fever, but there are no scientific evidences for its anti-dengue activity; hence we intended to investigate the anti-vir ... | 2013 | 24307765 |
| dengue virus type 2: protein binding and active replication in human central nervous system cells. | an increased number of dengue cases with neurological complications have been reported in recent years. the lack of reliable animal models for dengue has hindered studies on dengue virus (denv) pathogenesis and cellular tropism in vivo. we further investigate the tropism of denv for the human central nervous system (cns), characterizing denv interactions with cell surface proteins in human cns cells by virus overlay protein binding assays (vopba) and coimmunoprecipitations. in vopba, three membr ... | 2013 | 24302878 |
| mechanism for maturation-related reorganization of flavivirus glycoproteins. | flaviviruses, such as dengue, west nile, and yellow fever viruses, assemble as fusion-incompetent particles and subsequently undergo a large reorganization of their glycoprotein envelope resulting in formation of mature infectious virions. here we used a combination of three-dimensional cryo-electron tomography and two-dimensional image analysis to study pleomorphic maturation intermediates of dengue virus 2. icosahedral symmetries of immature and mature regions within one particle were mismatch ... | 2014 | 24252771 |
| modulation of enzymatic activity of dengue virus nonstructural protein ns3 nucleoside triphosphatase/helicase by poly(u). | the nonstructural protein 3 (ns3) appears to be the most promising target for anti-flavivirus therapy because of its multiple enzymatic activities that are indispensable for virus replication. ns3 of dengue virus type 2 (den2) is composed of two domains, a serine protease in the n-terminal domain (ns3pro) and rna-stimulated nucleoside triphosphatase (ntpase)/rna helicase at the c-terminus (ns3h). ns3 plays an important role in viral replication and the coordinated regulation of all the catalytic ... | 2013 | 24228882 |
| a novel dengue virus inhibitor, bp13944, discovered by high-throughput screening with dengue virus replicon cells selects for resistance in the viral ns2b/ns3 protease. | dengue virus (denv) causes disease globally, resulting in an estimated 25 to 100 million new infections per year. no effective denv vaccine is available, and the current treatment is only supportive. thus, there is an urgent need to develop therapeutic agents to cure this epidemic disease. in the present study, we identified a potential small-molecule inhibitor, bp13944, via high-throughput screening (hts) of 60,000 compounds using a stable cell line harboring an efficient luciferase replicon of ... | 2013 | 24145533 |
| olive baboons: a non-human primate model for testing dengue virus type 2 replication. | this study evaluated the use of a non-human primate, the olive baboon (papio anubis), as a model of dengue infection. olive baboons closely resemble humans genetically and physiologically and have been used extensively for assessing novel vaccine formulations. | 2013 | 24139794 |
| a chimeric dengue virus vaccine using japanese encephalitis virus vaccine strain sa14-14-2 as backbone is immunogenic and protective against either parental virus in mice and nonhuman primates. | the development of a safe and efficient dengue vaccine represents a global challenge in public health. chimeric dengue viruses (denv) based on an attenuated flavivirus have been well developed as vaccine candidates by using reverse genetics. in this study, based on the full-length infectious cdna clone of the well-known japanese encephalitis virus live vaccine strain sa14-14-2 as a backbone, a novel chimeric dengue virus (named chindenv) was rationally designed and constructed by replacement wit ... | 2013 | 24109223 |
| high-level expression of functionally active dengue-2 non-structural antigen 1 production in escherichia coli. | detection of nonstructural protein (ns1) is an important diagnostic marker during acute phase of dengue infection. not only for diagnostic purpose, the protein had important role in vaccine design as well, as a candidate for studying virus assembly and maturation. various researchers employed different expression systems and strategies for recombinant ns1 protein production. attempts to express ns1 protein in prokaryotic and yeast expression system result in formation of insoluble protein which ... | 2013 | 24089673 |
| inhibitory effects of curcumin on dengue virus type 2-infected cells in vitro. | curcumin, a traditional chinese and indian treatment for many diseases, has recently been found to alter the in vitro infection processes of various viruses, including hepatitis c virus, human immunodeficiency virus, coxsackievirus, and japanese encephalitis virus. the present study evaluated the cellular effects of curcumin in an in vitro (cellular) infection model of dengue virus. within a dose range of 10 to 30 μm and a treatment period of 24 hours, the cytotoxicity of curcumin was low, as de ... | 2014 | 24081825 |
| lipidated dengue-2 envelope protein domain iii independently stimulates long-lasting neutralizing antibodies and reduces the risk of antibody-dependent enhancement. | dengue virus is a mosquito-transmitted virus that can cause self-limiting dengue fever, severe life-threatening dengue hemorrhagic fever and dengue shock syndrome. the existence of four serotypes of dengue virus has complicated the development of an effective and safe dengue vaccine. recently, a clinical phase 2b trial of sanofi pasteur's cyd tetravalent dengue vaccine revealed that the vaccine did not confer full protection against dengue-2 virus. new approaches to dengue vaccine development ar ... | 2013 | 24069487 |
| generation and characterization of a rat monoclonal antibody against the rna polymerase protein from dengue virus-2. | dengue virus (denv) rna replication requires 2 viral proteins, non-structural protein 3 (ns3) and ns5. ns5 consists of 2 functional domains: a methyltransferase (mtase) domain involved in rna cap formation and located in the amino terminal region and a rna-dependent rna polymerase domain essential for virus replication and located in the carboxyl terminal region. to gain additional insight into the structural interactions between viral proteins and cellular factors involved in denv rna replicati ... | 2014 | 24063571 |
| dengue virus infection induces autophagy: an in vivo study. | we and others have reported that autophagy is induced by dengue viruses (dvs) in various cell lines, and that it plays a supportive role in dv replication. this study intended to clarify whether dv infection could induce autophagy in vivo. furthermore, the effect of dv induced autophagy on viral replication and dv-related pathogenesis was investigated. | 2013 | 24011333 |
| evaluation of protective efficacy using a nonstructural protein ns1 in dna vaccine-loaded microspheres against dengue 2 virus. | dengue virus results in dengue fever or severe dengue hemorrhagic fever/dengue shock syndrome in humans. the purpose of this work was to develop an effective antidengue virus delivery system, by designing poly (dl-lactic-co-glycolic) acid/polyethylene glycol (plga/peg) microspheres using a double-emulsion solvent extraction method, for vaccination therapy based on locally and continuously sustained biological activity. nonstructural protein 1 (ns1) in deoxyribonucleic acid (dna) vaccine-loaded p ... | 2013 | 23990724 |
| space constrained homology modelling: the paradigm of the rna-dependent rna polymerase of dengue (type ii) virus. | protein structure is more conserved than sequence in nature. in this direction we developed a novel methodology that significantly improves conventional homology modelling when sequence identity is low, by taking into consideration 3d structural features of the template, such as size and shape. herein, our new homology modelling approach was applied to the homology modelling of the rna-dependent rna polymerase (rdrp) of dengue (type ii) virus. the rdrp of dengue was chosen due to the low sequenc ... | 2013 | 23986788 |
| the ph dependence of flavivirus envelope protein structure: insights from molecular dynamics simulations. | the flavivirus membrane fusion is triggered by the acid ph of the endosomes after virus endocytosis. the proposed mechanism involves changes in the protonation state of conserved histidine residues of the e protein present in the viral surface that undergoes a series of structural rearrangements that result in the fusion between the endosome and viral bilayers. we studied the ph dependence of e protein rearrangements of dengue virus type 2, used as a model, in the ph range experimented by the vi ... | 2014 | 23964620 |
| vector competence of aedes albopictus and aedes aegypti (diptera: culicidae) for den2-43 and new guinea c virus strains of dengue 2 virus. | the vector competence of aedes albopictus and aedes aegypti with regard to den2-43 and new guinea c (ngc) virus strains of dengue 2 viruses was assessed and compared. the infection and dissemination rate and distribution of den2-43 antigens in orally infected ae. albopictus was investigated using the reverse transcription polymerase chain reaction and an indirect immunofluorescence assay. to better understand the initial infection, dissemination and transmission of these viral strains in vector ... | 2013 | 23962388 |
| chemistry and antiviral activity of arrabidaea pulchra (bignoniaceae). | the aim of the present work was to carry out a bioguided isolation of antiviral chemical constituents from an ethanol extract of leaves from arrabidaea pulchra (cham.) sandwith (eeapl) that had shown in vitro activity in a previous screening using dna and rna viruses. the activity of eepal was evaluated against the dna viruses human herpesvirus 1 (hsv-1) and vaccinia virus western reserve (vacv-wr) as well as against the rna viruses murine encephalomyocarditis virus (emcv), and dengue virus 2 (d ... | 2013 | 23959197 |
| reduced sphingosine kinase 1 activity in dengue virus type-2 infected cells can be mediated by the 3' untranslated region of dengue virus type-2 rna. | sphingosine kinase 1 (sphk1) is a lipid kinase with important roles including regulation of cell survival. we have previously shown reduced sphk1 activity in cells with an established dengue virus type-2 (denv-2) infection. in this study, we examined the effect of alterations in sphk1 activity on denv-2 replication and cell death and determined the mechanisms of the reduction in sphk1 activity. chemical inhibition or overexpression of sphk1 after established denv-2 infection had no effect on inf ... | 2013 | 23939980 |
| isolation of midgut escape mutants of two american genotype dengue 2 viruses from aedes aegypti. | several studies have shown that american genotype dengue 2 viruses (denv2) have reduced viral fitness in the mosquito vector, aedes aegypti, compared to other denv2 genotypes. diminished replication efficiency or inability to efficiently traverse membrane barriers encompassing organs such as the midgut or salivary glands are considered major factors negatively impacting viral fitness in the mosquito. | 2013 | 23937713 |
| effects of cocktail of four local malaysian medicinal plants (phyllanthus spp.) against dengue virus 2. | the absence of commercialized vaccines and antiviral agents against dengue has made the disease a major health concern around the world. with the current dengue virus transmission rate and incidences, the development of antiviral drugs is of vital need. the aim of this project was to evaluate the possibility of developing a local medicinal plant, phyllanthus as an anti-dengue agent. | 2013 | 23889893 |
| dengue virus photo-inactivated in presence of 1,5-iodonaphthylazide (ina) or amt, a psoralen compound (4'-aminomethyl-trioxsalen) is highly immunogenic in mice. | two novel methods of dengue virus inactivation using iodonaphthyl azide (ina) and aminomethyl trioxsalen (amt) were compared with traditional virus inactivation by formaldehyde. the amt inactivated dengue-2 virus retained its binding to a panel of 5 monoclonal antibodies specific for dengue-2 envelope protein, whereas inactivation by formaldehyde and ina led to 30-50% decrease in binding. all three inactivated viruses elicited high level virus neutralizing antibodies in vaccinated mice. however, ... | 2013 | 23835446 |
| serologic evidence of flavivirus infection in bats in the yucatan peninsula of mexico. | we captured 140 bats of seven species in merida city in the yucatan peninsula of mexico in 2010. serum was collected from each bat and assayed by plaque reduction neutralization test (prnt) using six flaviviruses: west nile virus, st. louis encephalitis virus, and dengue viruses 1-4. flavivirus-specific antibodies were detected in 26 bats (19%). the antibody-positive bats belonged to three species: the pallas's long-tongued bat (glossophaga soricina), jamaican fruit bat (artibeus jamaicensis), a ... | 0 | 23778622 |
| prevalence of antibodies to alphaviruses and flaviviruses in free-ranging game animals and nonhuman primates in the greater congo basin. | vector-borne and zoonotic pathogens have comprised a significant proportion of the emerging infectious diseases in humans in recent decades. the role of many wildlife species as reservoirs for arthropod-borne viral pathogens is poorly understood. we investigated the exposure history of various african wildlife species from the congo basin to mosquito-borne flaviviruses and alphaviruses by testing archived serum samples. sera from 24 african forest buffalo (syncerus caffer nanus), 34 african elep ... | 2013 | 23778608 |
| complete genome sequencing and evolutionary analysis of indian isolates of dengue virus type 2. | dengue is the most important arboviral infection of global public health significance. it is now endemic in most parts of the south east asia including india. though dengue virus type 2 (denv-2) is predominantly associated with major outbreaks in india, complete genome information of indian denv-2 is not available. in this study, the full-length genome of five denv-2 isolates (four from 2001 to 2011 and one from 1960), from different parts of india was determined. the complete genome of the indi ... | 2013 | 23756811 |
| dengue virus activates membrane trail relocalization and ifn-α production by human plasmacytoid dendritic cells in vitro and in vivo. | dengue displays a broad spectrum of clinical manifestations that may vary from asymptomatic to severe and even fatal features. plasma leakage/hemorrhages can be caused by a cytokine storm induced by monocytes and dendritic cells during dengue virus (denv) replication. plasmacytoid dendritic cells (pdcs) are innate immune cells and in response to virus exposure secrete ifn-α and express membrane trail (mtrail). we aimed to characterize pdc activation in dengue patients and their function under de ... | 2013 | 23755314 |
| the influence of dengue virus serotype-2 infection on aedes aegypti (diptera: culicidae) motivation and avidity to blood feed. | dengue virus (denv) is transmitted by aedes aegypti, a species that lives in close association with human dwellings. the behavior of denv-infected mosquitoes needs further investigation, especially regarding the potential influence of denv on mosquito biting motivation and avidity. | 2013 | 23755202 |
| rock is involved in vimentin phosphorylation and rearrangement induced by dengue virus. | our previous study showed that dengue virus 2 (denv2) infection induces rearrangement of vimentin into dense structures at the perinuclear area. however, the underlying mechanism of this phenomenon is poorly characterized. in the present work, we found that vimentin and ser71 phosphorylated vimentin display similar distributions in denv2-infected cells. denv2 infection also induced rock activation and phosphorylation of vimentin at ser71 as the denv2 infection progressed. furthermore, ser71 phos ... | 0 | 23737341 |
| differentially expressed genes of human microvascular endothelial cells in response to anti-dengue virus ns1 antibodies by suppression subtractive hybridization. | it has been previously shown that anti-dengue virus (denv) nonstructural protein ns1 antibodies could act as autoantibodies that direct against one or more of the host's own proteins, which has potential implications for dengue hemorrhagic fever pathogenesis. in the present study, we have employed suppression subtractive hybridization (ssh) to identify the differentially expressed genes from human microvascular endothelial cells (hmec-1) in response to anti-dengue virus type 2 ns1 antibodies (an ... | 2013 | 23697814 |
| [an inverted-repeat rna construct for silencing dengue virus type 2 pre-membrane gene suppresses viral replication in bhk-21 cells]. | to evaluate the anti-viral effects of a plasmid expressing an inverted-repeat rna targeting dengue virus type-2 (denv-2) pre-membrane (prm) gene. | 2013 | 23688980 |
| t helper (th) 1 and th2 cytokine expression profile in dengue and malaria infection using magnetic bead-based bio-plex assay. | dengue and malaria infections are two very common vector-borne diseases annually affecting millions of people around the world. both diseases show a variety of clinical presentations, ranging from mild symptoms of dengue fever (df) to severe dengue hemorrhagic fever (dhf) in dengue infection, and low and high parasitemia in malaria infection. t helper (th)1 and th2 cytokine expressions in mild and severe forms of dengue virus type-2 (denv-2) and plasmodium falciparum infection, were compared to ... | 2013 | 23682435 |
| total chemical synthesis of dengue 2 virus capsid protein via native chemical ligation: role of the conserved salt-bridge. | the dengue capsid protein c is a highly basic alpha-helical protein of ~100 amino acid residues that forms an emphipathic homodimer to encapsidate the viral genome and to interact with viral membranes. the solution structure of dengue 2 capsid protein c (den2c) has been determined by nmr spectroscopy, revealing a large dimer interface formed almost exclusively by hydrophobic residues. the only acidic residue (glu87) conserved in the capsid proteins of all four serotypes of dengue virus forms a s ... | 2013 | 23673222 |
| inhibition of enveloped viruses infectivity by curcumin. | curcumin, a natural compound and ingredient in curry, has antiinflammatory, antioxidant, and anticarcinogenic properties. previously, we reported that curcumin abrogated influenza virus infectivity by inhibiting hemagglutination (ha) activity. this study demonstrates a novel mechanism by which curcumin inhibits the infectivity of enveloped viruses. in all analyzed enveloped viruses, including the influenza virus, curcumin inhibited plaque formation. in contrast, the nonenveloped enterovirus 71 r ... | 2013 | 23658730 |
| inhibition of dengue virus 2 replication by artificial micrornas targeting the conserved regions. | dengue virus (denv), a mosquito-borne flavivirus, causes serious diseases and threatens public health in tropical and subtropical areas worldwide. rna interference (rnai) is a prevailing strategy for antiviral therapy. in this paper, 6 single artificial micrornas (amirnas) targeting the highly conserved regions of the denv-2 genome were identified and inhibited virus replication efficiently. then, effective tandem amirnas targeting 2 different denv-2 genome regions were constructed and expressed ... | 2013 | 23651254 |
| viperin is induced following dengue virus type-2 (denv-2) infection and has anti-viral actions requiring the c-terminal end of viperin. | the host protein viperin is an interferon stimulated gene (isg) that is up-regulated during a number of viral infections. in this study we have shown that dengue virus type-2 (denv-2) infection significantly induced viperin, co-incident with production of viral rna and via a mechanism requiring retinoic acid-inducible gene i (rig-i). viperin did not inhibit denv-2 entry but denv-2 rna and infectious virus release was inhibited in viperin expressing cells. conversely, denv-2 replicated to higher ... | 2013 | 23638199 |
| differential expression of apoptosis related genes in selected strains of aedes aegypti with different susceptibilities to dengue virus. | aedes aegypti is the principal vector of dengue viruses worldwide. we identified field collected insects with differential susceptibility to dengue-2 virus (denv-2) and used isofemale selection to establish susceptible and refractory strains based on midgut infection barriers. previous experiments had identified higher expression of apoptosis-related genes in the refractory strain. to identify potential molecular mechanisms associated with denv susceptibility, we evaluated the differential expre ... | 2013 | 23593426 |
| mutation of the dengue virus type 2 envelope protein heparan sulfate binding sites or the domain iii lateral ridge blocks replication in vero cells prior to membrane fusion. | using an infectious cdna clone we engineered seven mutations in the putative heparan sulfate- and receptor-binding motifs of the envelope protein of dengue virus serotype 2, strain 16681. four mutant viruses, kk122/123ee, e202k, g304k, and kkk305/307/310eee, were recovered following transfection of c6/36 cells. a fifth mutant, kk291/295ee, was recovered from c6/36 cells with a compensatory e295v mutation. all mutants grew in and mediated fusion of virus-infected c6/36 cells, but three of the mut ... | 2013 | 23571092 |
| age-dependent effects of oral infection with dengue virus on aedes aegypti (diptera: culicidae) feeding behavior, survival, oviposition success and fecundity. | aedes aegypti is the main vector of dengue, a disease that is increasing its geographical range as well as incidence rates. despite its public health importance, the effect of dengue virus (denv) on some mosquito traits remains unknown. here, we investigated the impact of denv-2 infection on the feeding behavior, survival, oviposition success and fecundity of ae. aegypti females. | 2013 | 23555838 |
| circulation of different lineages of dengue virus 2, genotype american/asian in brazil: dynamics and molecular and phylogenetic characterization. | the american/asian genotype of dengue virus type 2 (denv-2) was introduced into the americas in the 80's. although there is no data showing when this genotype was first introduced into brazil, it was first detected in brazil in 1990. after which the virus spread throughout the country and major epidemics occurred in 1998, 2007/08 and 2010. in this study we sequenced 12 denv-2 genomes obtained from serum samples of patients with dengue fever residing in são josé do rio preto, são paulo (sjrp/sp), ... | 2013 | 23533624 |
| activatable optical probes for the detection of enzymes. | the early detection of many human diseases is crucial if they are to be treated successfully. therefore, the development of imaging techniques that can facilitate early detection of disease is of high importance. changes in the levels of enzyme expression are known to occur in many diseases, making their accurate detection at low concentrations an area of considerable active research. activatable fluorescent probes show immense promise in this area. if properly designed they should exhibit no si ... | 0 | 23519774 |
| vectorial capacity of aedes aegypti for dengue virus type 2 is reduced with co-infection of metarhizium anisopliae. | aedes aegypti, is the major dengue vector and a worldwide public health threat combated basically by chemical insecticides. in this study, the vectorial competence of ae. aegypti co-infected with a mildly virulent metarhizium anisopliae and fed with blood infected with the denv-2 virus, was examined. | 2013 | 23505581 |
| the synergistic effect of combined immunization with a dna vaccine and chimeric yellow fever/dengue virus leads to strong protection against dengue. | the dengue envelope glycoprotein (e) is the major component of virion surface and its ectodomain is composed of domains i, ii and iii. this protein is the main target for the development of a dengue vaccine with induction of neutralizing antibodies. in the present work, we tested two different vaccination strategies, with combined immunizations in a prime/booster regimen or simultaneous inoculation with a dna vaccine (pe1d2) and a chimeric yellow fever/dengue 2 virus (yf17d-d2). the pe1d2 dna va ... | 2013 | 23472186 |
| [biological properties of virus dengue-3 strains isolated during the epidemic ocurred in havana, 2001-2002]. | during dengue epidemics in cuba, an increase in clinical severity with the epidemics progression in time, particularly in secondary infections, have been frequently observed. it is considered that this increase could be related with genetic changes in the circulating virus. | 2012 | 23444637 |
| chloroquine inhibits dengue virus type 2 replication in vero cells but not in c6/36 cells. | dengue viruses are the most important arthropod-borne viruses in terms of morbidity and mortality in the world. since there is no dengue vaccine available for human use, we have set out to investigate the use of chloroquine as an antiviral drug against dengue. chloroquine, an amine acidotropic drug known to affect intracellular exocytic pathways by increasing endosomal ph, was used in the in vitro treatment of vero and c6/36 cells infected with dengue virus type 2 (denv-2). real-time rt-pcr and ... | 2013 | 23431254 |
| dicer-2 processes diverse viral rna species. | rna silencing pathways play critical roles in gene regulation, virus infection, and transposon control. rna interference (rnai) is mediated by small interfering rnas (sirnas), which are liberated from double-stranded (ds)rna precursors by dicer and guide the rna-induced silencing complex (risc) to targets. although principles governing small rna sorting into risc have been uncovered, the spectrum of rna species that can be targeted by dicer proteins, particularly the viral rnas present during an ... | 2013 | 23424633 |
| structure and dynamics of the monomer of protein e of dengue virus type 2 with unprotonated histidine residues. | the surface of the dengue virus is composed of 180 copies of a multifunctional envelope glycoprotein that acts at several stages of viral infection. when the virus is in the endosome, these glycoproteins undergo major conformational rearrangements owing to the protonation of histidine side chains. this protonation allows for the formation of trimers, thereby triggering fusion between the viral and the host membranes. in this study, we examined the behavior of a monomer of this key protein contai ... | 2013 | 23420360 |
| effects of ribavirin and hydroxyurea on oral infection of aedes aegypti (l.) with dengue virus. | this study was conducted to determine the inhibitory effects of ribavirin and hydroxyurea on dengue virus replication in aedes aegypti mosquitoes. female ae. aegypti mosquitoes were infected with dengue-2 virus and fed ribavirin at a dose of 0.3 mg/ml and/or hydroxyurea at a dose of 6 mg/ml via artificial membrane feeding technique. the virus in infected mosquitoes was isolated using c6/36 cell culture. peroxidase-antiperoxidase (pap) staining was used to detect dengue-infected c6/36 cells and t ... | 2012 | 23413698 |
| transgene-mediated suppression of the rna interference pathway in aedes aegypti interferes with gene silencing and enhances sindbis virus and dengue virus type 2 replication. | rna interference (rnai) is the major innate antiviral pathway in aedes aegypti that responds to replicating arboviruses such as dengue virus (denv) and sindbis virus (sinv). on the one hand, the mosquito's rnai machinery is capable of completely eliminating denv2 from ae. aegypti. on the other, transient silencing of key genes of the rnai pathway increases replication of sinv and denv2, allowing the viruses to temporally overcome dose-dependent midgut infection and midgut escape barriers (meb) m ... | 0 | 23331493 |
| diversion of stress granules and p-bodies during viral infection. | rna granules are structures within cells that impart key regulatory measures on gene expression. two general types of rna granules are conserved from yeast to mammals: stress granules (sgs), which contain many translation initiation factors, and processing bodies (p-bodies, pbs), which are enriched for proteins involved in rna turnover. because of the inverse relationship between appearance of rna granules and persistence of translation, many viruses must subvert rna granule function for replica ... | 2013 | 23290869 |
| virus-like particles displaying envelope domain iii of dengue virus type 2 induce virus-specific antibody response in mice. | currently, dengue represents one of the most significant arboviral disease worldwide, for which a vaccine is not yet available. persistent challenges in live viral dengue vaccines have sparked a keen interest in exploring non-replicating dengue vaccines. we have examined the feasibility of using the methylotrophic yeast pichia pastoris to develop a chimeric vaccine candidate displaying the dengue virus type-2 (denv-2) envelope domain iii (ediii), implicated in host receptor binding and in the in ... | 2013 | 23261049 |
| epimedium koreanum nakai water extract exhibits antiviral activity against porcine epidermic diarrhea virus in vitro and in vivo. | porcine epidemic diarrhea virus (pedv) causes diarrhea of pigs age-independently and death of young piglets, resulting in economic loss of porcine industry. we have screened 333 natural oriental herbal medicines to search for new antiviral candidates against pedv. we found that two herbal extracts, kiom 198 and kiom 124, contain significant anti-ped viral effect. kiom 198 and kiom 124 were identified as epimedium koreanum nakai and lonicera japonica thunberg, respectively. the further plaque and ... | 2012 | 23259003 |
| induction of ifnα or il-12 depends on differentiation of thp-1 cells in dengue infections without and with antibody enhancement. | appropriate induction of the early th1 cytokine il-12 is a critical defense directed against viral infection. we have previously shown that different viruses elicited either il-12 or ifnα dependent th1 reactions. using dengue-2 virus, we sought to explore how dengue-2 induced il-12 or ifnα expression by monocytic and its derived dendritic cells. | 2012 | 23216989 |
| synchrony of sylvatic dengue isolations: a multi-host, multi-vector sir model of dengue virus transmission in senegal. | isolations of sylvatic dengue-2 virus from mosquitoes, humans and non-human primates in senegal show synchronized multi-annual dynamics over the past 50 years. host demography has been shown to directly affect the period between epidemics in other pathogen systems, therefore, one might expect unsynchronized multi-annual cycles occurring in hosts with dramatically different birth rates and life spans. however, in senegal, we observe a single synchronized eight-year cycle across all vector species ... | 2012 | 23209867 |
| new pockets in dengue virus 2 surface identified by molecular dynamics simulation. | one of the factors limiting the search of new compounds based on the structure of target proteins involved in diseases is the limited amount of target structural information. great advances in the search for lead compounds could be achieved to find new cavities in protein structures that are generated using well established computational chemistry tools. in the case of dengue, the discovery of pockets in the crystallographic structure of the e protein has contributed to the search for lead compo ... | 2013 | 23197323 |
| inhibition of cellular autophagy deranges dengue virion maturation. | autophagy is an important component of the innate immune response, directly destroying many intracellular pathogens. however, some pathogens, including several rna viruses, subvert the autophagy pathway, or components of the pathway, to facilitate their replication. in the present study, the effect of inhibiting autophagy on the growth of dengue virus was tested using a novel inhibitor, spautin-1 (specific and potent autophagy inhibitor 1). inhibition of autophagy by spautin-1 generated heat-sen ... | 2012 | 23175363 |
| [screening and identification of dengue virus type 2-specific antigens]. | to screen and identify dengue virus type 2 specific antigens and establish an enzyme-linked immunosorbent assay (elisa) for detecting dengue virus type 2 antibody. | 2012 | 23174601 |
| filovirus research in gabon and equatorial africa: the experience of a research center in the heart of africa. | health research programs targeting the population of gabon and equatorial africa at the international center for medical research in franceville (cirmf), gabon, have evolved during the years since its inception in 1979 in accordance with emerging diseases. since the reemergence of ebola virus in central africa, the cirmf "emerging viral disease unit" developed diagnostic tools and epidemiologic strategies and transfers of such technology to support the response of the national public health syst ... | 2012 | 23170174 |
| weather-driven variation in dengue activity in australia examined using a process-based modeling approach. | the impact of weather variation on dengue transmission in cairns, australia, was determined by applying a process-based dengue simulation model (densim) that incorporated local meteorologic, entomologic, and demographic data. analysis showed that inter-annual weather variation is one of the significant determinants of dengue outbreak receptivity. cross-correlation analyses showed that densim simulated epidemics of similar relative magnitude and timing to those historically recorded in reported d ... | 2012 | 23166197 |
| dengue virus type 2 modulates endothelial barrier function through cd73. | dengue hemorrhagic fever is characterized by a unique vascular leakage syndrome. the mechanisms of endothelial barrier dysfunction in dengue hemorrhagic fever are not well understood. we examined the modulation of endothelial barrier function in dengue virus type 2 (denv2) infections using primary human umbilical vein endothelial cells. we demonstrated that the increase in endothelial barrier function within 72 hours after denv2 infection is mediated by type i interferon-dependent cd73 up-regula ... | 2012 | 23149581 |
| novel antiviral activity of baicalein against dengue virus. | dengue is a serious arboviral disease currently with no effective antiviral therapy or approved vaccine available. therefore, finding the effective compound against dengue virus (denv) replication is very important. among the natural compounds, bioflavonoids derived mainly from plants are of interest because of their biological and medicinal benefits. | 2012 | 23140177 |