Publications

in vitro packaging of phage t3 dna.		1978	664260
effect of temperature on the transcription by bacteriophage t3-induced rna polymerase.	bacteriophage t3-induced rna polymerase is rapidly inactivated at 42 degrees c. addition of t3 dna delays this process for 30 s and reduces the rate with which the enzyme activity is lost indicating that a labile binary complex between t3 dna and polymerase must have been formed. the ternary complex between t3-specific rna polymerase, t3 dna, and nascent rna chains obtained when the enzyme is incubated with t3 dna, gtp, atp, and utp is stable to heat (42 degrees c) and only slowly inactivated by ...	1979	545912
transcription by bacteriophage t3-induced rna polymerase in the presence of kcl and dimethylsulfoxide.		1979	442703
termination of transcription by bacteriophage t3 rna polymerase: homogeneous 3'-terminal oligonucleotide sequence of in vitro t3 rna polymerase transcripts.	rna was synthesized in vitro from a t3 dna template by t3 rna polymerase and subsequently separated into seven discrete size classes (molecular weights ranging between 0.21 x 10(6) and 6.2 x 10(6)) by electrophoresis in polyacrylamide slab gels. rnase t1-generated 3'-terminal oligonucleotide fragments were then selectively isolated from either the unfractionated total rna or the gel-purified specific transcripts by chromatography on columns of dihydroxyboryl-cellulose. sequence analysis of these ...	1979	388430
[effect of temperature on formation of lysozyme in e. coli crt 266 (dnab ts) after infection with bacteriophage t3].	lysozyme formation induced by bacteriophage t3 was studied in the ts-mutant e. coli crt 266 (dnabts) and in the wild-type e. coli cr 34--45 (dnab+) at different temperatures. it was found that lysozyme was formed in e. coli crt 266, however, no lysozyme synthesis took place at 41.5 degrees c. these results indicate that the expression of the lysozyme gene is disturbed in the ts-mutant at 41.5 degrees c.	1978	382720
inactivation of escherichia coli by near-ultraviolet light and 8-methoxypsoralen: different responses of strains b/r and k-12.	a series of escherichia coli k-12 ab1157 strains with normal and defective deoxyribonucleic acid repair capacity were more resistant to treatment with 8-methoxypsoralen (8-mop) and near-ultraviolet light (nuv) than a comparable series of strains from the b/r wp2 family although sensitivities to 254-nm ultraviolet light were closely similar. the difference was most marked with strains deficient in both excision and postreplication repair (uvra reca). the hypothesis that the internal level of 8-mo ...	1979	378972
properties and biosynthesis of cyclopropane fatty acids in escherichia coli.	the lipid phase transition of escherichia coli phospholipids containing cyclopropane fatty acids was compared with the otherwise homologous phospholipids lacking cyclopropane fatty acids. the phase transitions (determined by scanning calorimetry) of the two preparations were essentially identical. infection of e. coli with phage t3 inhibited cyclopropane fatty acid formation over 98%, whereas infection with mutants which lack the phage coded s-adenosylmethionine cleavage enzyme had no effect on ...	1979	374358
different restriction of bacteriophages t3 and t7 by p1-lysogenic cells and the role of the t3-coded samase.	the intracellular growth of the phages t3 and t7 is restricted in the presence of the escherichia coli prophage p1. phage t3 has a higher ability to express its genome and to damage the host cell than t7. this partial protection of t3 against p1 restriction is due to the t3-coded samase, an enzyme which degrades s-adenosylmethionine, the cofactor of the p1 restriction endonuclease. since we did not observe dna cleavage in vivo, we conclude that the in vivo action of the p1 nuclease is limited to ...	1977	345634
further characterization of bacteriophage t3-induced ribonucleic acid polymerase. studies on the size of in vitro transcripts and interaction of t3 rna polymerase with t3 dna.		1977	330532
protein kinase of bacteriophage t7. 2. properties, enzyme synthesis in vitro and regulation of enzyme synthesis and activity in vivo.	protein kinase, which was isolated from cells infected with t7, is indeed a viral gene product. this is shown by dna-dependent synthesis in vitro. the protein kinase transfers phosphate from atp to seryl or threonyl residues in protein. the enzyme has only a relative requirement for magnesium ions, but is only active at low ionic strength. the best substrate is lysozyme. t7 protein kinase activity is not stimulated by cyclic 3':5'-amp and/or cyclic 3':5'-gmp. the t7 protein kinase carries -- sh ...	1975	240695
[use of gen5 and gen6 ts-mutants of phage t3 as indicators of inhibitors of dna synthesis].	in an enzyme-specific drug screening system nalidixic acid and 3'-ftdr, inhibitors of dna synthesis, both reduce the growth of wild type and temperature-sensitive point mutants of phage t3 with different efficiencies. the wild type shows the strongest sensitivity against the drugs, while an exonuclease mutant is the most insensitive variant. the dna polymerase mutants exhibit an intermediate degree of inhibition. the anthracycline antibiotics violamycin bi and adriblastin which preferentially in ...	1979	232593
cross-resistance of escherichia coli b/r to cis-platinum (ii) diamminochloride, uv light and alkylating agents.	gradual transfers of the strain escherichia coli b/r on m9 agar with increasing concentrations of cis-platinum (ii) diamminochloride (cis-pt(ii)) yielded a resistant strain sm 405 capable of growing on liquid m9 medium containing 250 mum cis-pt(ii). the parent strain escherichia coli b/r is completely inhibited in both division and growth at cis-pt(ii) concentrations as low as 30 mum. the resistant mutant has a longer doubling time than the parent strain. no other differences were found between ...	1975	170174
colivirus-t3-coded s-adenosylmethionine hydrolase.	bacteriophage t3 induces an enzyme activity which hydrolyzes s-adenosylmethionine. this s-adenosylmethionine hydrolase is interesting, not only because of its unique activity, but also because the protein has to overcome host restriction [f. w. studier and n. r. movva (1976) j. virol. 19, 136-145]. s-adenosylmethionine hydrolase was purified to homogeneity using affinity chromatography on s-adenosylhomocysteine-sepharose. the enzyme occurs in two forms, a and b. form a consists of the viral pept ...	1979	110588
[effect of temperature on rna synthesis in escherichia coli crt 266 (dna bts) following infection with bacteriophage t3].	infection of the temperature-sensitive e. coli crt 266 (dnabts) with t3-phages at the temperature of 30 degrees c and 35 degrees c, respectively, induced t3-specific rna synthesis with a maximum rate at 7 min (30 degrees c) and 4.5 min (35 degrees c) after infection. at temperatures above 40 degrees c no t3-induced rna synthesis could be observed. infection of e. coli cr 34--45 (dnab+) with t3 phages at 30 degrees c, 35 degrees c and at temperatures above 40 degrees c, however, produced t3-speci ...	1979	94483