Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
|---|
| patterns of gene expression in the core of spemann's organizer and activin-treated ectoderm in cynops pyrrhogaster. | the presumptive pharyngeal endoderm region of the cynops early gastrula induces head or trunk-tail structures in sandwich culture. activin-treated ectoderm can mimic this phenomenon at least at the histological level. the patterns of expression of organizer-specific genes were examined to compare these two inductive materials at the molecular level. a chordin cdna clone from cynops pyrrhogaster (cychd) was isolated by reverse transcription-polymerase chain reaction (rt-pcr). cychd mrna was first ... | 1998 | 9639361 |
| development of the endolymphatic sac and duct in the japanese red-bellied newt, cynops pyrrhogaster. | the development and maturation of the endolymphatic sac (es) and duct (ed) were studied in the newt cynops pyrrhogaster. the es first appears as an oval capsule at the dorsal-medial tip of the otic vesicle at stage 39, about 11 days after oviposition. the es consists of polymorphous epithelial cells with a minimum of cytoplasm. the intercellular space (is) between the epithelial cells is narrow and has a smooth surface. at stage 44, the size of the es increases as many vacuoles in the is become ... | 1998 | 9606061 |
| difference between dorsal and ventral iris in lens producing potency in normal lens regeneration is maintained after dissociation and reaggregation of cells from the adult newt, cynops pyrrhogaster. | in wolffian lens regeneration, lentectomized newt eye can produce a new lens from the dorsal marginal iris, but the ventral iris has never shown such capabilities. to investigate the difference of lens regenerating potency between dorsal and ventral iris epithelium at the cellular level, a transplantation system using cell reaggregates was developed. two methods were devised for preparing the reaggregates from pigmented iris epithelial cells. one was rotating cells in an agar-coated multiplate o ... | 1998 | 9563906 |
| changes in cyclin b during oocyte maturation and early embryonic cell cycle in the newt, cynops pyrrhogaster: requirement of germinal vesicle for mpf activation. | when full-grown oocytes of the newt cynops pyrrhogaster were treated with progesterone in o-r2 solution containing antibiotics, approximately 85% of the oocytes completed meiosis synchronously. maturation-promoting factor (mpf) activity appeared just before germinal vesicle breakdown (gvbd) and the oocytes maintained high mpf activity throughout metaphase i and metaphase ii of meiosis. a slight decrease of mpf activity was observed at the first polar body emission. the distribution of cyclin b1 ... | 1998 | 9520324 |
| ultrastructure of the endolymphatic sac in the larva of the japanese red-bellied newt cynops pyrrhogaster. | the ultrastructure of the endolymphatic sac (es) of the late stage larva of the japanese red-bellied newt, cynops pyrrhogaster (stage 57), was examined by light and transmission electron microscopy. the two endolymphatic sacs are located at the dorsal-medial side of the otic vesicle on the dorsal-lateral side of the midbrain in the cranial cavity. the wall of the sac is composed of a layer of cubical epithelial cells with loose, interposed intercellular spaces. the sac contains a large luminal c ... | 1998 | 9477311 |
| [elucidation of the mechanism of retinal degeneration and regeneration and the prospects for its clinical application]. | in order to obtain the basic knowledge necessary to develop therapeutical intervention for blindness due to the damaged retina and optic nerve, the mechanism of retinal degeneration and regeneration in an amphibian model, cynops pyrrhogaster, was studied. in the retinal degenerative process following enucleation and reimplantation of the eye ball, evidence was found for active cell death of neural retinal cells. as the degeneration proceeded, musashi, an ribonucleic acid (rna)-binding protein, s ... | 1997 | 9436360 |
| innervation of two peptidergic (substance p and calcitonin gene-related peptide) nerves in the cerebral arteries and choroid plexus of the japanese newt (triturus pyrrhogaster). | the pattern of cerebrovascular substance p (sp) and calcitonin gene-related peptide (cgrp) immunoreactive (-ir) innervation was investigated in the newt. sp-ir nerves supplying the cerebral arterial tree and choroid plexus were positive for cgrp, but negative for vasoactive intestinal polypeptide or neuropeptide y. it is suggested that cerebrovascular sp- and cgrp-ir axons are sensory in nature. the supply of sp- and cgrp-ir nerves to the major cerebral arteries is relatively poor. nevertheless, ... | 1997 | 9409510 |
| female-attracting pheromone in newt cloacal glands. | it has been postulated that male newts emit pheromones that attract females of the same species. female newts of the species cynops pyrrhogaster were attracted to water in which sexually active conspecific males had been kept, but not to water in which abdominal gland-ablated males had been kept, indicating that the attracting pheromone was secreted by or through the abdominal gland of the cloaca. an attempt has been made to isolate and characterize the female-attracting pheromone in the abdomin ... | 1997 | 9370206 |
| changes in microtubule structures during the first cell cycle of physiologically polyspermic newt eggs. | the unfertilized egg of the newt, cynops pyrrhogaster, has a second meiotic spindle at the animal pole and numerous cortical cytasters. after physiologically polyspermic fertilization, all sperm nuclei incorporated into the egg develop sperm asters, and the cortical cytasters change into bundles of cortical microtubules. the size of the sperm asters in the animal hemisphere is approximately 5.6-fold larger than that in the vegetal hemisphere. only one sperm nucleus moves toward the center of the ... | 1997 | 9136124 |
| effect of protein phosphatase inhibitors on cleavage furrow formation in newt eggs: inhibition of normal furrow formation and concomitant induction of furrow-like dents. | the effects of three protein phosphatase inhibitors, okadaic acid, calyculin a and tautomycin, on the formation of cleavage furrows and the induction of furrow-like dents in the egg of the newt, cynops pyrrhogaster, were examined. solutions of the individual compound were injected into the animal hemisphere of one of the two presumptive blastomere regions of the embryo during the first cleavage. injection of a solution containing any of the chemicals often disturbed the formation of a normal fur ... | 1997 | 9108337 |
| primary structures of sperm-specific basic nuclear proteins and gene expression in japanese newt, cynops pyrrhogaster. | electrophoretic analyses of acid extracts from mature sperm of newt, cynops pyrrhogaster, on acid/urea/triton x-100 polyacrylamide gel showed the exclusive occurrence of sperm-specific nuclear basic proteins (sbps), which moved faster than somatic histones on the gel. these sbps were eluted separately by reversed phase-high-performance liquid chromatography as two large peaks and a few small peaks. of these, only the small peaks disappeared with treatment of the acid extracts with alkaline phosp ... | 1997 | 9041126 |
| involvement of endogenous prolactin in the expression of courtship behavior in the newt, cynops pyrrhogaster. | in order to ascertain the involvement of endogenous prolactin (prl) in the expression of courtship behavior by the male newt, cynops pyrrhogaster, antiserum against homologous prl was administered to male newts, which had been exhibiting courtship behavior in the field and had been taken to the laboratory. the effect of the antiserum on the preference of terrestrial over aquatic habitats also was investigated. within 24 hr of the first injection of antiserum, both incidence and frequency of cour ... | 1996 | 8998963 |
| radioimmunoassay of a newt sex pheromone, sodefrin, and the influence of hormones on its level in the abdominal gland. | a specific and sensitive radioimmunoassay (ria) was developed for the measurement of sodefrin, a female-attracting decapeptide pheromone of the red-bellied newt, cynops pyrrhogaster. sodefrin synthesized according to the amino acid sequence of native sodefrin, isolated from the abdominal glands of the cloaca of the male newt, was used as a reference standard. an antiserum to sodefrin was produced by immunizing a rabbit with synthetic sodefrin that was extended on its c-terminus with cys coupled ... | 1996 | 8954769 |
| activin treated urodele ectoderm: a model experimental system for cardiogenesis. | the tissue interactions which comprise the inductive phenomena associated with urodele heart morphogenesis are relatively well understood. in order to take full advantage of the experimental potential of this system formulation of an in vitro tissue culture system would be very helpful. herein are described conditions for culturing cynops pyrrhogaster early gastrula ectoderm tissue in the presence of the peptide growth factor activin. two-week old explant cultures frequently displayed beating he ... | 1996 | 8877444 |
| [new sex pheromone isolated from the abdominal glands of male newt, cynops pyrrhogaster]. | sodefrin, a novel decapeptide which attracts female newts, cynops pyrrhogaster was isolated from the abdominal gland of the male of the same species. synthetic sodefrin was tested for its activity with sexually active females and males and sexually inert females. it attracted only sexually active females. the effect of sodefrin was blocked by a bilateral nostril plugging with cotton balls soaked in melted vaseline. immunoelectronmicroscopic study using antiserum against sodefrin revealed that so ... | 1995 | 8763038 |
| comparison of mauthner cell size in sexually developed and undeveloped male red-bellied newts. | differences in mauthner (m) cell size were examined in sexually developed and undeveloped male red-bellied newts, cynops pyrrhogaster. the mean areas of nuclei and cell bodies of m cells and mean maximum and minimum diameters of the cell bodies in the sexually developed males were significantly larger than those in the sexually undeveloped ones. in the hypophysectomized male newts, all these parameters were not significantly different from those in the sexually undeveloped ones. these values wer ... | 1995 | 8586962 |
| non-invasive assessment of otolith formation during development of the japanese red-bellied newt, cynops pyrrhogaster. | pre-mated adult female newts and embryos have been flown on the international microgravity laboratory-2 (iml-2) space shuttle flight in 1994 (wiederhold et al., 1992b). with the specimens available from this flight, the calcification of otoliths, ulna, radius and backbone of the flown larvae and adult newts were analyzed. the experiments presented here studied the development of the otoliths on the ground. otoliths of living newts, from embryo to adult, were observed in situ with the application ... | 1995 | 8575997 |
| hormonal induction of male courtship behavior in the japanese newt, cynops pyrrhogaster. | in the breeding season, the sexually mature male newt, cynops pyrrhogaster, vibrates the tail in front of the female at an early stage of courtship. effects of prolactin (prl), gonadotropin (gth), and sex steroids on this behavior were investigated in the male paired with the female receiving prl and gth. the behavior was elicited in the sexually inert male by injections of prl of bovine, ovine, or bullfrog origin and human chorionic gonadotropin or bullfrog lh and fsh in combination. the effect ... | 1993 | 8294119 |
| the egg nucleus regulates the behavior of sperm nuclei as well as cycling of mpf in physiologically polyspermic newt eggs. | the possible role of the egg nucleus in regulating the behavior of sperm nuclei and the cycling of maturation-promoting factor (mpf) was investigated in the physiologically polyspermic eggs of the newt cynops pyrrhogaster. many sperm entered all areas of the egg, but only one sperm pronucleus, the principal sperm pronucleus, moved to the center of the animal hemisphere to form a zygote nucleus with the egg pronucleus. all sperm and egg pronuclei synthesized dna, but the zygote nucleus completed ... | 1993 | 8224532 |
| motion sickness in amphibians. | we explored the question of whether amphibians get motion sickness by exposing anurans (frogs) and urodeles (salamanders) to the provocative stimulus of parabolic aircraft flight. animals were fed before flight, and the presence of vomitus in their containers after flight was used to indicate motion-induced emesis. none of the species that we studied vomited during the 8 to 10 parabolas of each flight. however, at least one specimen from each of the anuran species rana rugosa, rana nigromaculata ... | 1993 | 8216158 |
| hormonal control of response to and secretion of sex attractants in japanese newts. | hormonal control of sexual chemoattraction in the newt, cynops pyrrhogaster, was investigated by a water preference test. sexually inert newts injected with prolactin (prl) and human chorionic gonadotropin (hcg) in combination preferred the water in which newts of the opposite sex had been kept, whereas saline-injected specimens did not. this indicates that prl and hcg enhance the response of the newts to attractant(s) secreted by the opposite sex. the water in which prl plus hcg-treated newts h ... | 1994 | 8190778 |
| ultrastructure of the olfactory organ of the newt, cynops pyrrhogaster. | the ultrastructure of the nasal sacs of the japanese newt, cynops pyrrhogaster, was studied by scanning and transmission electron microscopy. the paired nasal sacs of the newt are dorsoventrally flattened with a lateral nasal sinus off the main cavity of each sac. throughout each sac is a series of ridges and grooves. in the main cavity, sensory epithelium with ciliated and microvillous receptor cells lines the grooves, and a thin, ciliated non-sensory epithelium lines the ridges. secretory glan ... | 1994 | 8059972 |
| initiation and stimulation of spermatogenesis in vitro by mammalian follicle-stimulating hormone in the japanese newt, cynops pyrrhogaster. | in order to elucidate the molecular mechanisms by which spermatogenesis is regulated, especially the roles of hormones and somatic cells in the initiation and promotion of spermatogenesis, we developed an organ culture system with a chemically defined medium. when newt testes fragments rich in secondary spermatogonia were cultured in control medium for three weeks, most of the testicular cysts still remained as secondary spermatogonia. on the other hand, in the medium supplemented with follicle- ... | 1994 | 7981029 |
| sodefrin: a female-attracting peptide pheromone in newt cloacal glands. | a decapeptide called sodefrin was isolated from the abdominal gland of the cloaca of the male red-bellied newt, cynops pyrrhogaster. the native peptide, as well as the synthetic one, had a female-attracting activity. sodefrin was found in the apical portion of the epithelial cells of the abdominal gland. sodefrin is apparently species specific because it did not attract females of cynops ensicauda. this is the first amphibian pheromone to be identified and the first peptide pheromone identified ... | 1995 | 7886452 |
| mammalian follicle-stimulating hormone stimulates dna synthesis in secondary spermatogonia and sertoli cells in organ culture of testes fragments from the newt, cynops pyrrhogaster. | we previously showed in organ culture of testes fragments from cynops pyrrhogaster that mammalian follicle-stimulating hormone (fsh) stimulates secondary spermatogonia to differentiate into primary spermatocytes. in this report, we demonstrate in organ culture that fsh stimulates dna synthesis in secondary spermatogonia and sertoli cells: the numbers of secondary spermatogonia and sertoli cells incorporating 5-bromo-2'-deoxyuridine (brdu) throughout the culture period in the presence of fsh were ... | 1994 | 7881917 |
| promotion of differentiation of newt primary spermatocytes into spermatids by mammalian fsh via sertoli cells. | our previous studies showed that mammalian follicle-stimulating hormone (fsh) promoted the differentiation of primary spermatocytes into elongated spermatids in organ culture of testes fragments from cynops pyrrhogaster. to elucidate the mechanism of fsh action, in this study the testes in the primary spermatocyte stage were dissociated and fractionated into germ and somatic cells, the latter comprising more than 80% sertoli cells. radioreceptor assays showed that fsh bound to somatic cells, ver ... | 1995 | 7673875 |
| proliferation of newt spermatogonia by mammalian fsh via sertoli cells in vitro. | our previous studies showed that mammalian follicle-stimulating hormone (fsh) stimulated the proliferation and differentiation of secondary spermatogonia into primary spermatocytes in organ culture of testes fragments from cynops pyrrhogaster. to elucidate how fsh stimulates spermatogonial proliferation, we studied the interaction of spermatogonia with somatic cells in the presence or absence of fsh in cultures of aggregated cells derived from fractionated cell populations. when spermatogonia or ... | 1995 | 7673874 |
| development and application of a homologous radioimmunoassay for xenopus prolactin. | a specific and sensitive homologous radioimmunoassay (ria) for xenopus prolactin (xprl) was developed. prl isolated from x. laevis pituitary glands was used for generating antiserum in a rabbit, for radioligand and for the standard. pituitary homogenates and plasma from adult xenopus produced displacement curves parallel to the xprl standard. plasma from hypophysectomized xenopus showed negligible cross-reactivity. purified prls from other amphibian species such as the bullfrog (rana catesbeiana ... | 1995 | 7657154 |
| calcium-containing, smooth-surfaced endoplasmic reticulum and vacuoles in cells of the blastopore-forming region during gastrulation of the newt, cynops pyrrhogaster. | ultrastructural changes in cells of the blastopore-forming region (bfr) were examined during gastrulation of the newt, cynops pyrrhogaster. smooth-surfaced endoplasmic reticulum (ser), tubular in shape and with two types of vacuole, empty vacuoles and multilamellar vacuoles, appeared in cells of the bfr at the beginning of formation of the blastopore. the extent of the tubular ser increased during formation of the blastopore. with the deepening of the blastoporal groove, the tubular ser and mult ... | 1995 | 7645763 |
| the morphogenic features of otoconia during larval development of cynops pyrrhogaster, the japanese red-bellied newt. | otoconia are calcified protein matrices within the gravity-sensing organs of the vertebrate vestibular system. mammalian otoconia are barrel-shaped with triplanar facets at each end. reptilian otoconia are commonly prismatic or fusiform in shape. amphibians have all three otoconial morphologies, barrel-shaped otoconia within the utricle, with prismatic and fusiform otoconia in the saccule. scanning electron microscopy revealed a sequential appearance of all three otoconial morphologies during la ... | 1995 | 7642456 |
| development of the otolith organs and semicircular canals in the japanese red-bellied newt, cynops pyrrhogaster. | the sequence in which the otoliths and semicircular canals and their associated sensory epithelia appear and develop in the newt are described. three-dimensional reconstruction of serial sections through the otic vesicle of newt embryos from stages 31 through 58 demonstrate the first appearance, relative position and growth of the otoliths. a single otolith is first seen in stage 33 embryos (approximately 9 days old); this splits into separate utricular and saccular otoliths at stage 40 (13 days ... | 1995 | 7642454 |
| atomic force microscope observations of otoconia in the newt. | calcitic and aragonitic otoconia from the japanese red-bellied newt, cynops pyrrhogaster, were examined using an atomic force microscope. the surface structure of both otoconial polymorphs consisted of arrays of elements approximately 50 nm in diameter. elements were generally round and were separated by shallow depressions of no more than 20 nm. the elements are suggested to be single crystals of calcium carbonate. the relationship of these observations to theories of otoconial genesis is discu ... | 1995 | 7559167 |
| neuroepithelial endocrine and nervous system in the respiratory tract of cynops pyrrhogaster with special reference to the distribution of nitric oxide synthase and serotonin. | the respiratory tract of urodeles harbours an intramural nerve network comprising an innervated system of neuroepithelial endocrine (nee) cells. however, striking differences have been noted between phylogenetically closely related species. zamboni- or formaldehyde-fixed whole-mount preparations and sections of the saclike lungs of a japanese salamander, cynops salamander, cynops pyrrhogaster, have been investigated for the immunocytochemical detection of nitric oxide synthase (nos), serotonin ( ... | 1994 | 7529073 |
| [differentiation of granulocytes in the newt (triturus pyrrhogaster), with special reference to the granule formation (author's transl)]. | 1980 | 7424490 | |
| electron microscope study of vertebrate liver innervation. | liver fine structure was studied in various groups of vertebrates to reveal intrahepatic nerves. nerve fibers were found in the connective tissue of the liver in all mammals, birds, and reptiles studied (japanese monkey, crab-eating monkey, rabbit, guinea pig, rat, golden hamster, pigeon, japanese quail, and turtle, pseudemys scripta). nerve fibers also made direct contact with hepatocytes in these animals except for the rat and the golden hamster. intrahepatic nerves were rare or absent in amph ... | 1981 | 7316686 |
| meiosis of primary spermatocytes and early spermiogenesis in the resultant spermatids in newt, cynops pyrrhogaster in vitro. | dissociated spermatogenic cells were cultivated within the collagen matrix at low cell density. the largest cell type in the culture was identified as the primary spermatocytes by their size and the morphological characteristics revealed by ultra-thin sections. chromosome analysis showed that about 90% of the cells examined were either in first or second meiosis. within the collagen matrix, the fates of 282 single primary spermatocytes at meiotic stage in diakinesis or metaphase were followed. i ... | 1981 | 7308609 |
| temporal pattern of cleavage and the onset of gastrulation in amphibian embryos developed from eggs with the reduced cytoplasm. | fertilized eggs of the japanese newt, cynops pyrrhogaster, were divided into two or four equal-sized parts with fine glass rods before the first cleavage. in such cases one of the egg fragments, at least, proceeds to cleavage and gastrulates. the temporal pattern of cell division and the onset of gastrulation in such half or quarter embryos were investigated and compared with normal development. the following results were obtained: (1) desynchronization starts two divisions earlier in quarter em ... | 1981 | 7276823 |
| creeping locomotion of the endodermal cells dissociated from gastrulae of the japanese newt, cynops pyrrhogaster. | 1981 | 7238592 | |
| the photoreceptor cell in the pineal organ of the japanese common newt. | 1982 | 7151960 | |
| behaviour of annulate lamellae during the maturation of oocytes in the newt, cynops pyrrhogaster. | the distribution of annulate lamellae, electron-dense masses, rough endoplasmic reticulum, and golgi complexes in longitudinal sections of newt oocytes at several stages of progesterone-induced maturation was recorded with an electron microscope equipped with a drawing device. annulate lamellae in full-grown oocytes occur in close contact with electron-dense masses and the nuclear envelope and elsewhere. stacks of annulate lamellae increase in number for 6 h after progesterone treatment. meanwhi ... | 1982 | 7142895 |
| seasonal and geographical changes of spontaneous skin papillomas in the japanese newt cynops pyrrhogaster. | the occurrence of spontaneous skin papillomas in japanese newts (cynops pyrrhogaster) from niigata prefecture in northern japan was monitored over four seasons covering a period of 2 years. of 13,613 newts, 249 were found to possess papillomas. the percentage of newts with papillomas was highest in autumn, ranging from 1.93 to 5.45% of the total average. these values were more than four times as high as values obtained in the other three seasons (0.16 to 0.50%). a total of 12,167 newts were coll ... | 1982 | 7105040 |
| effect of hypophysectomy and starvation on the fine structure of vitellogenic oocytes in the newt, triturus pyrrhogaster (laur). | hypophysectomy and starvation in triturus pyrrhogaster result in a similar ultrastructural pattern of follicular atresia. the first detectable change in the oocytes is decrease in the size and number of microvilli. subsequently the yolk precursor bodies (ypb) disappear from the micropinocytotic vesicles and the mitochondria degenerate. finally the yolk platelets undergo decomposition. the smaller yolk platelets disintegrate by granulation, vacuolation and lobule formation. the larger platelets, ... | 1982 | 6892135 |
| seasonal changes in plasma testosterone and 5 alpha-dihydrotestosterone levels in the adult male newt, cynops pyrrhogaster pyrrhogaster. | seasonal changes in plasma levels of testosterone (t) and 5 alpha-dihydrotestosterone (dth) were examined in the adult male japanese red-bellied newt. the concentration of plasma t increased significantly (p less than 0.05) from january to february and decreased drastically from april to july when it reached its minimum. after the summer decline, plasma t increase rapidly from july to october (p less than 0.05) and reached its maximum in october. concerning the seasonal fluctuation of plasma dht ... | 1983 | 6884270 |
| the structure of the kidney of japanese newts, triturus (cynops) pyrrhogaster. | the construction of renal lobules in triturus (cynops) pyrrhogaster was studied by reconstruction from serial semithin sections, and the structure of nephrons, collecting ducts and ureters was investigated by means of light and electron microscopy. in t. pyrrhogaster the kidney was mesonephros in construction; renal lobules were arranged segmentally and each of them sent one ureter. male ureters ran caudally and met together before joining the wolffian duct. in renal lobules, long collecting duc ... | 1983 | 6837932 |
| the degradation of urate in liver peroxisomes. association of allantoinase with allantoicase in amphibian liver but not in fish and invertebrate liver. | allantoinase and allantoicase were co-purified from frog (rana catesbeiana) liver. the ratio of the two enzyme activities remained constant during purification and was unchanged by a variety of treatments of the purified enzyme. these results suggest that allantoinase and allantoicase are located in the same protein. it was found that the two hepatic enzyme activities are also associated with the same protein in other frogs (xenopus laevis and rana nigromacultata), tadpoles (r. catesbeiana), and ... | 1983 | 6833275 |
| studies on the development of melanophores in in vitro cultured amphibian neural plates. | various parts of neural plates of japanese newt (cynops pyrrhogaster) neurula embryos were cultured alone in drops of culture media (niu-twitty's balanced salt solution or modified leibovitz l-15 medium) with or without fetal calf serum (fcs). although none of the parts gave rise to melanophores in a medium without fcs, some produced melanophores in a medium with fcs. the localization of melanophore-producing areas in the neural plates corresponded to that of tada's (1944) findings. the assumpti ... | 1983 | 6825926 |
| cell surface properties of amphibian embryonic cells. | gastrula and neurula embryos of cynops pyrrhogaster were dissociated into cell suspensions with ethylenediaminetetraacetic acid (edta), trypsin, and solution. the cells were cultured in niu-twitty's balanced salt solution and their aggregates were examined histologically. the results indicated that the capacity of amphibian embryonic cells for aggregate formation, sorting out, and notochord differentiation was not suppressed by edta and alkali treatments. trypsin treatment, however, virtually su ... | 1980 | 6796637 |
| seasonal variation in pituitary gonadotropin in the adult male newt, cynops pyrrhogaster pyrrhogaster, revealed by isoelectric focusing technique and radioreceptor assay. | the seasonal variation in pituitary gonadotropin in the adult male newt, cynops pyrrhogaster pyrrhogaster was investigated by means of isoelectric focusing (ief) coupled with radioreceptor assay (rra), which employed anolis or xenopus testicular homogenates as receptors and 125i-rat fsh as radioligand. in the anolis rra system, the standard curve was obtained with 0.125-16 ng/tube of niamdd rat fsh i-3. purified preparations, chicken lh ief-1, chicken fsh agchd11113a and bullfrog basic gonadotro ... | 1981 | 6796397 |
| cadmium, copper and zinc-binding protein (metallothionein) in the liver of the water lizard triturus pyrrhogaster. | a low-mr copper (cu) and zinc (zn)-binding protein was present in the liver of a control water lizard triturus pyrrhogaster. cadmium (cd) accumulated in the liver of the animals after intramuscular injections of cd salt and was bound to cu- and zn-binding protein of a single isoform that had properties characteristic of metallothionein (mt). effects of cd loading on several essential elements in the liver and kidney were also determined. | 1984 | 6719500 |
| comparative study of extracellular fibrils on the ectodermal layer in gastrulae of five amphibian species. | previous studies have shown the presence of a network of extracellular fibrils on the inner surface of the ectodermal layer of the ambystoma maculatum gastrulae. the alignment of the network along the blastopore-animal pole axis has suggested that the network of fibrils guides the migrating mesodermal cells in gastrulae by contact guidance. we have also shown that these fibrils can be deposited on substrata by explanted embryonic fragments and that substrata conditioned in this manner support di ... | 1983 | 6602807 |
| lack of cellular differentiation with neoplastic-like proliferation in the limbs of the newt cynops pyrrhogaster. | we report a case of a neoplastic-like proliferation of epidermal cells in the limbs of an adult newt. the undifferentiated cell mass infiltrated the mesodermal tissues. possible effects of this event on limb regeneration are discussed. | 2003 | 6486729 |
| [evaluation of amputation techniques for the study of limb regeneration in the newt and toad]. | a comparison between forelimb amputations through the radius-ulna and the humerus was made in the adult newt, cynops pyrrhogaster pyrrhogaster and the young toad, xenopus laevis. newts regenerated their forelimbs after the amputations and the new parts resembled the old in structure and function. toads regenerated their forelimbs incompletely after the amputations. the new parts lacked joints and fingers showing a simply elongate pattern. the effect of re-amputation of exposed bone on the regene ... | 1984 | 6468505 |
| follicle-stimulating hormone (fsh) receptors in the testis of the newt, cynops pyrrhogaster, and comparison of temperature dependency of the receptors with those of the other vertebrates. | 1980 | 6245987 | |
| retinotopic analysis of fiber pathways is amphibians. i. the adult newt cynops pyrrhogaster. | the possibility that pathways of retinal fibers within the optic tract and the tectum of the adult newt are retinotopic was examined by selective labeling of the retinal fibers with horseradish peroxidase. within the optic tract fibers from the ventral, temporal and dorsal retinal quadrants were ordered from the dorsal to ventral edges of th optic tract. the nasal retinal fibers exhibited two different pathways. the fibers from the dorsonasal retina ran along the ventral edge of the optic tract, ... | 1981 | 6162523 |
| retinotopic analysis of fiber pathways in the regenerating retinotectal system of the adult newt cynops pyrrhogaster. | retinotopic analysis of the pathways of regenerating retinal fibers within the optic tract and in the tectum of an adult newt was performed by selective labeling of the retinal fibers with horseradish peroxidase. at the tenth week of regeneration, all the regenerating retinal fibers from different retinal quadrants had terminal arbors nearly at the parts of the tectum innervated normally by those quadrants. the pathways for individual retinal fibers, however, were greatly disorganized within the ... | 1981 | 6162521 |
| histochemical and electrophoretic studies of esterase in the regenerating forelimb of the adult newt, triturus pyrrhogaster. | 1967 | 6075515 | |
| in vitro analyses of wolffian lens regeneration: differentiation of the regenerating lens rudiment of the newt, triturus pyrrhogaster. | 1967 | 6075514 | |
| differentiation capacity of the marginal zone in the morula and blastula of triturus pyrrhogaster. | 1967 | 6075513 | |
| mast cells and histamine in a newt, triturus pyrrhogaster boié. | 1968 | 5711878 | |
| lymphosarcomatous disease of the newt, triturus pyrrhogaster. | 1970 | 5538539 | |
| ultrasturcture of the parathyroid gland of the newt, triturus pyrrhogaster (boié) in the spring season. | 1970 | 5468683 | |
| regional histone changes in embryos of the newt, triturus pyrrhogaster, during early development. | 1970 | 5459824 | |
| electron microscopic study on the parathyroid gland of the newt triturus pyrrhogaster (boié) in natural hibernation. | 1970 | 5442608 | |
| behaviour of histones and cytoplasmic basic proteins during embryogenesis of the japanese newt, triturus pyrrhogaster. | 1969 | 5408616 | |
| are the parathyroid glands necessary to the life of the newt, cynops pyrrhogaster? | 1969 | 5395934 | |
| an electron microscopic study on the guanophore in the iris of the newt, triturus pyrrhogaster (boié). | 1969 | 5395640 | |
| cultivation in vitro of the testis cord of the newt, triturus pyrrhogaster. | 1969 | 5392793 | |
| alkaline phosphatase activity in the regenerating limb of the adult newt triturus pyrrhogaster. | 1969 | 5384492 | |
| [the electron microscopic study on the functional area of the digestive epithelial cell membrane in newt, triturus pyrrhogaster]. | 1971 | 5290269 | |
| experiments on a spontaneously orginated visceral tumor in the newt, triturus pyrrhogaster. | 1970 | 5278142 | |
| ultrastructural change in the parathyroid gland of the phosphate treated newt, triturus pyrrhogaster (boié). | 1971 | 5168592 | |
| origin of histones, and relation between nuclear histones and cytoplasmic basic proteins in early development of japanese newt, triturus pyrrhogaster. | 1972 | 5037000 | |
| electron-microscopic observations on the finer structure of basophilic granulocytes of newt (triturus pyrrhogaster). | 2016 | 4960340 | |
| electrical coupling between fat cells in newt fat body and mouse brown fat. | white fat from the newt, triturus pyrrhogaster, fat body, and brown fat from the interscapular fat pad of newborn mice have been tested for the presence of low-resistance intercellular junctions. 42 pairs of amphibian fat cells and 15 pairs of mammalian brown fat cells were found to be "electrically coupled." in most of these cases intracellular deposition of a dye, niagara sky blue: 6b, was used to supplement and confirm direct observations of impalements. coupling was often difficult to find i ... | 1971 | 4938627 |
| wave of stiffness propagating along the surface of the newt egg during cleavage. | in the eggs of the newt, cynops (triturus) pyrrhogaster, change in stiffness of the cortex was measured in various regions at the time of the cleavage. measurements were performed by mitchison and swann's cell elastimeter method with a modification, in which two fine pipettes were attached to the surface of one egg at the same time, in order to compare the rigidity of two regions. the stiffness of the cortex changed very little before the start of the first cleavage. however, just before the app ... | 1974 | 4809240 |
| self-differentiation of the heart rudiment of the newt (cynops pyrrhogaster). | 1973 | 4797323 | |
| electron microscopic study on the parathyroid gland of the parathormone-injected newt, triturus pyrrhogaster (boie). | 1973 | 4740812 | |
| spontaneous epidermal tumor in an adult newt, cynops pyrrhogaster. | 1973 | 4689907 | |
| monoamine oxidase in the neurohypophysis of the newt (cynops pyrrhogaster pyrrhogaster), the toad (bufo bufo japonicus) and the tortoise (clemmys japonica). | 1972 | 4623962 | |
| merits of the japanese newt, cynops pyrrhogaster pyrrhogaster, as an experimental animal, especially for the study of transplantation immunity. | 1973 | 4609273 | |
| [neural control of the terminal of the hindgut of triturus pyrrhogaster]. | 1974 | 4477310 | |
| a nephroblastoma in a fire-bellied newt, cynops pyrrhogaster. | 1970 | 4321086 | |
| the mast cells in the brain of the triturus pyrrhogaster (boie). i. the histologic studies. | 1968 | 4181658 | |
| cell surface changes during cleavage of newt eggs: scanning electron microscopic studies. | scanning electron microscopic studies were carried out on changes of the cell surface during the first cleavage of eggs of the newt, cynopus (triturus) pyrrhogaster. a dense population of elongated microvilli, which initially covered a double line indicating the initial furrow and stress marks running on each side of it, became located inside the pigmented area a little apart from its border with the unpigmented area when this borderline was established. this finding, together with data of carbo ... | 2014 | 4039355 |
| differentiation and distribution of annulate lamellae in growing oocytes in the newt, cynops pyrrhogaster. | stages of oocyte development in cynops pyrrogaster are defined, and changes of annulate lamellae in their fine structure, number, sizes and locations during oogenesis are described. the results show that two different types of annulate lamellae occur during oogenesis. one type differentiates in or at the periphery of vesicle-rich cytoplasm at the early stages of vitellogenesis and increases in number and size. the maximum number of about 40 stacks per median section of oocyte is reached at the s ... | 1985 | 3998684 |
| effects of temperature on papilloma growth in the newt, cynops pyrrhogaster. | the effects of temperature on skin papillomas of newts (cynops pyrrhogaster) were studied. newts bearing these tumors were maintained for 30 weeks at 4 +/- 1 (s.e.), 10 +/- 1, 13 +/- 2, 25 +/- 1, and 30 +/- 1 degree. the diameters of the papillomas were measured externally. they decreased at 4, 25, and 30 degrees, and increased at 10 and 13 degrees. the effect of temperature on the tumor growth or regression began to appear from around the 10th week. furthermore, when the temperatures were chang ... | 1985 | 3971368 |
| comparative studies on the electric nature of amphibian gonadotropin. | the isoelectric focusing (ief) profiles of pituitary gonadotropic (gth) activity in six species of adult male amphibians were examined in addition to the newt and bullfrog. the gth activity was measured by two radioreceptor assay (rra) systems; one with xenopus laevis testicular homogenate as the receptor preparation, and the other with anolis carolinensis testicular homogenate, both employing 125i-labeled rat fsh as the radioligand, or by a radioimmunoassay (ria) system for bullfrog basic gth ( ... | 1985 | 3874809 |
| surface contraction of dividing newt eggs induced by lowering the external ionic strength or ph, or by trypsinization. | as the first cleavage progresses, newt (cynopus pyrrhogaster) eggs deprived of their vitelline membrane grow to expose the new, unpigmented surface to a saline medium full-strength steinberg solution). further exposure of these eggs to sucrose medium resulted in rapid and extensive contraction of the unpigmented surface. in the sucrose medium the contraction continued until the next cleavage, and eventually the eggs divided into many blastomeres. but partial reversal of the contraction was obser ... | 1986 | 3793777 |
| new translation system of mrna coding for neurotransmitter receptors using oocytes of the newt, cynops pyrrhogaster. | eel electroplax mrna was injected into oocytes of newts (cynops pyrrhogaster) and the injection induced synthesis of a nicotinic acetylcholine receptor in the oocyte membrane. the time course of the induction and dose-response relationship of the receptor were recorded electrophysiologically. the receptor responses and their developmental changes were similar to those of xenopus oocytes injected with eel mrna. newt oocytes lived much longer (7-10 days) than xenopus oocytes (3-4 days) under our e ... | 2013 | 3742236 |
| cell death during normal gastrulation in the newt, cynops pyrrhogaster. | cells falling off from ectoderm were observed in normally developing gastrulae of the newt, cynops pyrrhogaster, in light microscopic examination. these cells eventually died. the number of such necrotic cells per embryo varied from a few dozen to a few thousand. in embryos with many necrotic cells, the ectoderm was thin, the yolk plug large, and establishment of the neural plate was delayed compared with embryos with fewer necrotic cells. a neural tube of normal size was formed at the tail bud ... | 1986 | 3731278 |
| the spike component of the fertilization potential in the toad, bufo japonicus: changes during meiotic maturation and absence during cross-fertilization. | immature oocytes of the toad, bufo japonicus, inseminated between first- and second-meiotic metaphase, exhibited polyspermy. monospermy occurred when the oocytes had reached second-meiotic metaphase. electrical recording during insemination of the immature oocyte showed fast-rising and slow-rising spikes followed by a gradual shift to a positive membrane potential. the number of fast spikes in each oocyte corresponded well with the number of sperm observed in cytological sections. mature oocytes ... | 1987 | 3653525 |
| induction of ocular tumor by nickel subsulfide in the japanese common newt, cynops pyrrhogaster. | chemically induced ocular tumors in amphibians have not been reported previously. in the present study, nickel subsulfide (ni3s2) was administered to lentectomized japanese common newts, cynops pyrrhogaster, by a single injection into the posterior chamber of the right eye (40-100 micrograms ni3s2/newt). control newts received a similar injection of vehicle. malignant melanoma-like tumors developed in the injected eyes of seven of eight ni3s2-treated newts at 9 months (versus none of six control ... | 1987 | 3621207 |
| monoamine-containing basal cells in the taste buds of the newt triturus pyrrhogaster. | monoamine-containing cells were examined by fluorescence histochemistry and electron microscopy. two or three serotonin-like fluorescent cells were located just above the basal lamina and failed to reach the free surface of the taste bud. ultrastructurally this cell type was characterized by the presence of dense-cored vesicles and finger-like cytoplasmic processes. many characteristics of merkel cells were present. | 1987 | 3481961 |
| total dna transcription in vitro: a procedure to detect highly repetitive and transcribable sequences with trna-like structures. | total dnas from various animals were transcribed in vitro in a hela cell extract, and it was found that one to several discrete rnas were transcribed by rna polymerase iii. with tortoise (geoclemys reevessi) and newt (cynops pyrrhogaster), distinct 6.5s and 8s rnas were transcribed from these respective dnas. representative phage clones carrying the 6.5s and 8s rna genes were isolated from genomic libraries of these animals, and the sequences of these genes were determined. the 5' parts of highl ... | 1986 | 3455763 |
| factors related to the initiation of cell migration along the inner surface of the blastocoelic wall during amphibian gastrulation. | changes in the quantity of extracellular matrix on the inner surface of the blastocoelic wall (bw) and the adhesiveness of cells to fibronectin were examined in relation to the timing of initiation of cell migration along the bw during gastrulation of the newt, cynops pyrrhogaster. extracellular matrix fibrils were already abundantly present all over the inner surface of the bw prior to the beginning of cell migration. on the other hand, the adhesiveness of cells to fibronectin rapidly increased ... | 1988 | 3409322 |
| formation of germ layers and roles of the dorsal lip of the blastopore in normally developing embryos of the newt cynops pyrrhogaster. | three-dimensional relationships between tissues during the formation of germ layers were studied in sections of normally developing embryos of the newt, cynops pyrrhogaster. in gastrulae, the inner postinvolution layer was not in direct contact with the outer preinvolution layer as a result of the presence of an intervening layer of cells. only after the formation of the yolk plug, a narrow strip of primitive notochord, which consisted of columnar cells, established a close contact with the cent ... | 1988 | 3404106 |
| how is the flagellar length of mature sperm determined? i. comparison of flagellar growth in spermatids between newt and xenopus in vitro. | the kinetics of flagellar growth in round spermatids were compared between xenopus laevis and cynops pyrrhogaster in vitro, the latter of which has about 13 times longer flagella in mature sperm than the former. in both species, more than 90% of the spermatids derived from marked primary spermatocytes grew flagella. in xenopus the average flagellar length increased to 28 microns by the 6th day and then stopped growth, while in the newt, flagellar growth did not stop until reaching 107 microns in ... | 1988 | 3371423 |
| effect of prolactin on transcutaneous na transport in the japanese newt, cynops pyrrhogaster. | the effect of ovine prolactin (prl) on the electric parameters related to active na transport across the skin was investigated in the japanese newt, cynops pyrrhogaster. spring newts living in a pond or stream for breeding and autumn newts migrating from water to land for hibernation were collected and used for the experiments. autumn newt skin was found to have a higher transepithelial potential difference (tep) and higher resistances to the active na current (rna) and to the shunt pathway (rs) ... | 1988 | 3360287 |
| autoneuralization in the amphibian ectoderm--a species-specific and stage-specific phenomenon. | ectoderm from ambystoma is especially prone to undergo 'autoneuralization'. this assertion has led to the maxim that ectoderm from this species is unsuitable for studying cell differentiation. here we report that the degree of neuralization in cultured explants is stage-dependent. control explants from blastulae (stage 8-9) show no neuralization, while explants treated with licl (10 mm) give rise to neuralization in about 70% of the cases. this difference between control and experiment decreases ... | 1987 | 3311847 |
| mauthner neurons survive metamorphosis in anurans: a comparative hrp study on the cytoarchitecture of mauthner neurons in amphibians. | giant medullary neurons were revealed in adult xenopus laevis and rana esculenta following hrp injections to the spinal cord. these neurons were identified as mauthner neurons because (1) they have the same position and orientation as the larval mauthner neurons, i.e., they lie at the level of the viiith nerve root. (2) they have two large dendritic trees that for each species are similar to those of the larval mauthner neurons, (3) they are clearly distinguishable from other large reticular neu ... | 1986 | 3081602 |