Publications
| Title | Abstract | Year Filter | PMID(sorted descending) Filter |
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| assessment of the ecotoxicological risk of combined sewer overflows for an aquatic system using a coupled "substance and bioassay" approach. | very few tools are available for assessing the impact of combined sewer overflows (csos) on receiving aquatic environments. the main goal of the study was to assess the ecotoxicological risk of csos for a surface aquatic ecosystem using a coupled "substance and bioassay" approach. wastewater samples from the city of longueuil, canada cso were collected for various rainfall events during one summer season and analyzed for a large panel of substances (n = 116). four bioassays were also conducted o ... | 2015 | 25315929 |
| rainbow trout (oncorhynchus mykiss) elov15 and elov12 differ in selectivity for elongation of omega-3 docosapentaenoic acid. | the synthesis of the omega-3 long-chain polyunsaturated fatty acids (lcpufa) eicosapentaenoic acid (epa; 20:5n-3) and docosahexaenoic acid (dha; 22:6n--3) from dietary α-linolenic acid (ala; 18:3n--3) requires three desaturation and three elongation steps in vertebrates. the elongation of epa to docosapentaenoic acid (dpa; 22:5n-3) can be catalysed by the elongase enzymes elov15 or elov12, but further elongation of dpa to 24:5n-3, the penultimate precursor of dha, is limited to elov12, at least ... | 2014 | 25311171 |
| adverse and long-term protective effects following oil-adjuvanted vaccination against aeromonas salmonicida in rainbow trout. | prophylactic measures against aeromonas salmonicida subsp. salmonicida, the causative agent of furunculosis, have been an active field of research for decades, with studies mainly focused on atlantic salmon (salmo salar). in the present study we have examined the protective and adverse effects of mineral oil-adjuvanted injection vaccines on rainbow trout (oncorhynchus mykiss). a commercial vaccine and an experimental auto vaccine, as well as their respective adjuvant formulations alone were used ... | 2015 | 25281580 |
| microencapsulation of a putative probiotic enterobacter species, c6-6, to protect rainbow trout, oncorhynchus mykiss (walbaum), against bacterial coldwater disease. | flavobacterium psychrophilum is the causative agent of bacterial coldwater disease (bcwd), which has a major impact on salmonid aquaculture globally. an enterobacter species, c6-6, isolated from the gut of rainbow trout, oncorhynchus mykiss (walbaum), has been identified as a potential probiotic species providing protection against bcwd. this study examined the effects of alginate microencapsulation on the protective efficacy of c6-6 against bcwd in vivo when administered to rainbow trout fry or ... | 2016 | 25272249 |
| biological plausibility as a tool to associate analytical data for micropollutants and effect potentials in wastewater, surface water, and sediments with effects in fishes. | discharge of substances like pesticides, pharmaceuticals, flame retardants, and chelating agents in surface waters has increased over the last decades due to the rising numbers of chemicals used by humans and because many wwtps do not eliminate these substances entirely. the study, results of which are presented here, focused on associations of (1) concentrations of micropollutants in wastewater treatment plant (wwtp) effluents, surface waters, sediments, and tissues of fishes; (2) results of la ... | 2015 | 25260601 |
| development of a list of reference chemicals for evaluating alternative methods to in vivo fish bioaccumulation tests. | the aim to reduce the number of animals in experiments has highlighted the need to develop and validate nonanimal methods as alternatives to bioaccumulation studies using fish. the present study details a novel 3-tier approach to develop a list of reference compounds to aid this process. the approach was based on 1) the inclusion of relevant chemical classes supported by high-quality in vivo data for the bioconcentration factor (bcf), whole-body biotransformation rates (k(met)), and metabolism c ... | 2014 | 25244043 |
| complete genome sequence of flavobacterium psychrophilum strain csf259-93, used to select rainbow trout for increased genetic resistance against bacterial cold water disease. | the genome sequence of flavobacterium psychrophilum strain csf259-93, isolated from rainbow trout (oncorhynchus mykiss), consists of a single circular genome of 2,900,735 bp and 2,701 predicted open reading frames (orfs). strain csf259-93 has been used to select a line of rainbow trout with increased genetic resistance against bacterial cold water disease. | 2014 | 25237017 |
| histopathologic changes in disease-resistant-line and disease-susceptible-line juvenile rainbow trout experimentally infected with flavobacterium psychrophilum. | abstract a challenge to selectively breeding fish populations for improved disease resistance in aquaculture is an incomplete understanding of how artificial selection affects innate immunity at the host-pathogen level. the objective of this study was to determine whether rainbow trout oncorhynchus mykiss bred for differential susceptibility to bacterial cold-water disease exhibited altered tissue damage and cellular inflammatory response following experimental challenge with flavobacterium psyc ... | 2014 | 25229490 |
| comparison of the susceptibility of four rainbow trout strains to cold-water disease. | abstract susceptibility to cold-water disease was compared among four strains of rainbow trout oncorhynchus mykiss: arlee strain from ennis national fish hatchery, montana (al-en), the arlee strain from jocko river hatchery, montana (al-jr), a cold-water disease-resistant strain (wv), and the harrison-hofer strain (hh). bacterial challenges were either by bath or intraperitoneal injection (50 μl of 0.65 optical density). each strain was exposed at 75 d after hatch to either the csf 259-93 (idaho ... | 2014 | 25229487 |
| differences in igy gut absorption in gastric rainbow trout (oncorhynchus mykiss) and agastric common carp (cyprinus carpio) assessed in vivo and in vitro. | oral igy antibodies offer promising potential for passive immunization strategies. to evaluate barriers for successful igy plasma recovery after oral application in vivo, gastric rainbow trout and agastric common carp were comparatively assessed. a positive control that received a low dose of unspecific igy antibodies by intraperitoneal injection (0.0076 mg igy g bw(-1) d(-1); bw=body mass) was compared with an oral administration of 75 and 150 fold in rainbow trout (corresponding to 0.57 and 1. ... | 2015 | 25224559 |
| dietary fermentable fiber upregulated immune related genes expression, increased innate immune response and resistance of rainbow trout (oncorhynchus mykiss) against aeromonas hydrophila. | this trial was carried out to investigate the effects of dietary administration of vitacel(®), a commercial fermentable fiber, on immune related genes (lysozyme, tnfα and hsp70) expression, innate immune response and resistance of rainbow trout against aeromonas hydrophila. 120 healthy rainbow trout (81.65 ± 1.49 g) were distributed in six fiberglass tanks assigned to two treatments. the treatments were feeding rainbow trout with diets supplemented with 0 (control) or 10 g kg(-1) vitacel(®) for ... | 2014 | 25218276 |
| induction of anti-inflammatory cytokine expression by ipnv in persistent infection. | infectious pancreatic necrosis virus (ipnv) is the agent of a well-characterized acute disease that produces a systemic infection and high mortality in farmed fish species but also persistent infection in surviving fish after outbreaks. because viral persistence of susceptible mammal hosts appears to be associated with the modulation of anti-inflammatory cytokine expression, in this study we examined the expression levels of key pro- and anti-inflammatory cytokines in kidney and spleen of trout, ... | 2014 | 25193394 |
| ecotoxicological evaluation of sewage sludge contaminated with zinc oxide nanoparticles. | the objective of this work was to evaluate the ecotoxicological qualitative risk associated with the use of sewage sludge containing zn oxide nanoparticles (zno-nps) as soil amendment. a sludge-untreated soil and two sludge-treated soils were spiked with zno-nps (0-1,000 mg/kg soil). soil ecotoxicity was assessed with eisenia fetida (acute and sublethal end points), and the unfilterable and filterable (0.02 μm) soil leachates were tested with a battery of biomarkers using chlorella vulgaris, dap ... | 2014 | 25185842 |
| infectivity of piscirickettsia salmonis in immersion-bath exposed rainbow trout oncorhynchus mykiss (walbaum) fry. | 2015 | 25168060 | |
| purified deoxynivalenol or feed restriction reduces mortality in rainbow trout, oncorhynchus mykiss (walbaum), with experimental bacterial coldwater disease but biologically relevant concentrations of deoxynivalenol do not impair the growth of flavobacterium psychrophilum. | diets containing deoxynivalenol (don) were fed to rainbow trout oncorhynchus mykiss (walbaum) for 4 weeks followed by experimental infection (intraperitoneal) with flavobacterium psychrophilum (4.1 × 10(6) colony-forming units [cfu] ml(-1) ). mortality of rainbow trout fed either 6.4 mg kg(-1) don or trout pair-fed the control diet was significantly reduced (p < 0.05) in comparison with trout fed the control diet to apparent satiation (<0.1 mg kg(-1) don). in a second experiment, trout were fed ... | 2015 | 25160820 |
| diet type dictates the gut microbiota and the immune response against yersinia ruckeri in rainbow trout (oncorhynchus mykiss). | this study investigated the influence of the rainbow trout (oncorhynchus mykiss) commensal intestinal microbiota in connection to an experimental yersina ruckeri infection, the causative agent of enteric redmouth disease. one marine and one plant diet was administered to two different groups of rainbow trout. the plant-based diet gave rise to an intestinal microbiota dominated by the genera streptococcus, leuconostoc and weissella from phylum firmicutes whereas phylum proteobacteria/bacteroidete ... | 2014 | 25150450 |
| effect of dietary bovine lactoferrin on rainbow trout (oncorhynchus mykiss) fecundity and larval quality. | 2013 | 25141604 | |
| a putative serine protease, spssp1, from saprolegnia parasitica is recognised by sera of rainbow trout, oncorhynchus mykiss. | saprolegniosis, the disease caused by saprolegnia sp., results in considerable economic losses in aquaculture. current control methods are inadequate, as they are either largely ineffective or present environmental and fish health concerns. vaccination of fish presents an attractive alternative to these control methods. therefore we set out to identify suitable antigens that could help generate a fish vaccine against saprolegnia parasitica. unexpectedly, antibodies against s. parasitica were fou ... | 2014 | 25088077 |
| salmonids have an extraordinary complex type i ifn system: characterization of the ifn locus in rainbow trout oncorhynchus mykiss reveals two novel ifn subgroups. | fish type i ifns are classified into two groups with two (group i) or four (group ii) cysteines in the mature peptide and can be further divided into four subgroups, termed ifn-a, -b, -c, and -d. salmonids possess all four subgroups, whereas other teleost species have one or more but not all groups. in this study, we have discovered two further subgroups (ifn-e and -f) in rainbow trout oncorhynchus mykiss and analyzed the expression of all six subgroups in rainbow trout and brown trout salmo tru ... | 2014 | 25080482 |
| attempts at validating a recombinant flavobacterium psychrophilum gliding motility protein n as a vaccine candidate in rainbow trout, oncorhynchus mykiss (walbaum) against bacterial cold-water disease. | the flavobacterium psychrophilum gliding motility n (gldn) protein was investigated to determine its ability to elicit antibody responses and provide protective immunity in rainbow trout oncorhynchus mykiss (walbaum). gldn was pcr-amplified, cloned into pet102/d-topo, and expressed in escherichia coli. bacteria expressing recombinant gldn (rgldn) were formalin-inactivated and injected intraperitoneally (i.p.) into rainbow trout with freund's complete adjuvant (fca) in four separate studies that ... | 2014 | 25053267 |
| acute sensitivity of white sturgeon (acipenser transmontanus) and rainbow trout (oncorhynchus mykiss) to copper, cadmium, or zinc in water-only laboratory exposures. | the acute toxicity of cadmium, copper, and zinc to white sturgeon (acipenser transmontanus) and rainbow trout (oncorhynchus mykiss) were determined for 7 developmental life stages in flow-through water-only exposures. metal toxicity varied by species and by life stage. rainbow trout were more sensitive to cadmium than white sturgeon across all life stages, with median effect concentrations (hardness-normalized ec50s) ranging from 1.47 µg cd/l to 2.62 µg cd/l with sensitivity remaining consistent ... | 2014 | 25043712 |
| gill remodeling in three freshwater teleosts in response to high environmental ammonia. | the present study aimed to determine whether gill macro- and microstructure show compensatory responses in three freshwater fish differing in their sensitivity to high environmental ammonia (hea). the highly ammonia-sensitive salmonid oncorhynchus mykiss (rainbow trout), the less ammonia-sensitive cyprinid cyprinus carpio (common carp) and the highly ammonia-resistant cyprinid carassius auratus (goldfish) were used as test species and were exposed for 0 h (control), 3h, 12h, 24h, 48 h, 84 h and ... | 2014 | 25033244 |
| four cish paralogues are present in rainbow trout oncorhynchus mykiss: differential expression and modulation during immune responses and development. | suppressor of cytokine signalling (socs) family members are crucial in the control and attenuation of cytokine induced responses via activation of the jak/stat, tlr and nf-kb signalling pathways. socs proteins orchestrate the termination of many types of immune responses and are often the targets of microbial pathogens exploiting socs mechanisms to evade the host's immune response. through whole and lineage specific genome duplication events, the teleost cytokine/socs network is complex. not onl ... | 2014 | 25014904 |
| immunohistochemical localization of inflammatory cells and cell cycle proteins in the gills of loma salmonae infected rainbow trout (oncorhynchus mykiss). | microsporidial gill diseases particularly those caused by loma salmonae incur significant economic losses to the salmonid aquaculture industry. the gill responses to infection include the formation of xenomas and the acute hyperplastic inflammatory responses once the xenomas rupture releasing infective spores. the aim of this work was to characterize the inflammatory responses of the gill to both the presence of the xenomas as well as the hyperplasia associated with l. salmonae infection in the ... | 2014 | 24979224 |
| increased ventilation by fish leads to a higher risk of parasitism. | fish are common intermediate hosts of trematode cercariae and their gills can potentially serve as important sites of penetration by these larval stages. we experimentally tested the hypothesis that volume of ventilation flow across the gills contributes to acquisition of these parasites by fish. we manipulated the intensity of ventilation by using different oxygen concentrations. | 2014 | 24954703 |
| transient transfection of cho cells using linear polyethylenimine is a simple and effective means of producing rainbow trout recombinant ifn-γ protein. | a practical method was developed for the transient transfection of chinese hamster ovary (cho) cells with 25 kda linear polyethylenimine (pei) then optimal culture conditions determined for the production of rainbow trout (oncorhynchus mykiss) ifn-γ recombinant protein. we found that culture temperature had a significant impact upon recombinant protein yield, with best results being obtained at 32 °c. however the amount of serum added to the culture medium had no effect upon recombinant ifn-γ (r ... | 2015 | 24897997 |
| susceptibility of koi and yellow perch to infectious hematopoietic necrosis virus by experimental exposure. | infectious hematopoietic necrosis virus (ihnv) is a novirhabdoviral pathogen that originated in western north america among anadromous pacific salmonids. severe disease epidemics in the late 1970s resulting from ihnv's invasion into farmed rainbow trout oncorhynchus mykiss in north america, asia, and europe emphasized ihnv's ability to adapt to new hosts under varying rearing conditions. yellow perch perca flavescens and koi carp cyprinus carpio (hereafter, "koi") are aquaculture-reared fish tha ... | 2014 | 24895860 |
| investigation of the link between broodstock infection, vertical transmission, and prevalence of flavobacterium psychrophilum in eggs and progeny of rainbow trout and coho salmon. | the etiological agent of bacterial coldwater disease (bcwd), flavobacterium psychrophilum, can be transmitted both vertically and horizontally. outbreaks of bcwd can result in significant losses in salmonid aquaculture. reduction of outbreaks in fry may be possible through implementation of a management strategy in which progeny of heavily infected broodstock are culled from the general population. diagnostic assays to quantify f. psychrophilum concentrations in tissue samples and confirm presen ... | 2014 | 24895859 |
| in vitro and in vivo effects of phytoestrogens on protein turnover in rainbow trout (oncorhynchus mykiss) white muscle. | soybeans and other legumes investigated as fishmeal replacements in aquafeeds contain phytoestrogens capable of binding to and activating estrogen receptors. estradiol has catabolic effects in salmonid white muscle, partially through increases in protein turnover. the current study determines whether phytoestrogens promote similar effects. in rainbow trout (oncorhynchus mykiss) primary myocyte cultures, the phytoestrogens genistein, daidzein, glycitein, and r- and s-equol reduced rates of protei ... | 2014 | 24874080 |
| developmental oestrogen exposure differentially modulates igf-i and tnf-α expression levels in immune organs of yersinia ruckeri-challenged young adult rainbow trout (oncorhynchus mykiss). | intensified aquaculture has strong impact on fish health by stress and infectious diseases and has stimulated the interest in the orchestration of cytokines and growth factors, particularly their influence by environmental factors, however, only scarce data are available on the gh/igf-system, central physiological system for development and tissue shaping. most recently, the capability of the host to cope with tissue damage has been postulated as critical for survival. thus, the present study as ... | 2014 | 24874061 |
| chronic sensitivity of white sturgeon (acipenser transmontanus) and rainbow trout (oncorhynchus mykiss) to cadmium, copper, lead, or zinc in laboratory water-only exposures. | chronic toxicity of cadmium, copper, lead, or zinc to white sturgeon (acipenser transmontanus) and rainbow trout (oncorhynchus mykiss) was evaluated in water-only exposures started with newly hatched larvae or approximately 1-mo-old juveniles. the 20% effect concentration (ec20) for cadmium from the sturgeon tests was higher than the ec20 from the trout tests, whereas the ec20 for copper, lead, or zinc for the sturgeon were lower than those ec20s for the trout. when the ec20s from the present st ... | 2014 | 24862826 |
| mercury and selenium concentrations in muscle tissue of different species of predatory freshwater fish and correlation between these elements. | concentrations of total mercury and selenium were determined in 49 and 42 muscle tissue samples, respectively, of six species of predatory freshwater fish, dace (leuciscus leuciscus), pike perch (sander lucioperca), pike (esox lucius), european catfish (silurus glanis), rainbow trout (oncorhynchus mykiss) and asp (aspius aspius). muscle selenium concentration did not correlate with the corresponding total mercury concentration (r² < 0.198) in all examined predatory fish species. there was an inv ... | 2012 | 24779785 |
| anti-oxidative defences are modulated differentially in three freshwater teleosts in response to ammonia-induced oxidative stress. | oxidative stress and the antioxidant response induced by high environmental ammonia (hea) were investigated in the liver and gills of three freshwater teleosts differing in their sensitivities to ammonia. the highly ammonia-sensitive salmonid oncorhynchus mykiss (rainbow trout), the less ammonia sensitive cyprinid cyprinus carpio (common carp) and the highly ammonia-resistant cyprinid carassius auratus (goldfish) were exposed to 1 mm ammonia (as nh4hco3) for 0 h (control), 3 h, 12 h, 24 h, 48 h, ... | 2014 | 24740135 |
| the proximal promoter of a novel interleukin-8-encoding gene in rainbow trout (oncorhynchus mykiss) is strongly induced by cebpa, but not nf-κb p65. | interleukin-8 (il8) is an immediate-early chemokine that has been well characterized in several fish species. ten il8 gene variants have already been described in rainbow trout, but none of their promoters has structurally been defined or functionally characterized in teleost fish. to uncover key factors regulating il8 expression, we intended to functionally characterize an il8 promoter from rainbow trout. incidentally, we isolated a novel il8 gene variant (il8-g). it is structurally highly simi ... | 2014 | 24721762 |
| morphometric evaluation of interrenal gland and kidney macrophages aggregates in normal healthy rainbow trout (oncorhynchus mykiss) and after bacterial challenge with yersinia ruckeri. | macrophage aggregates (ma) occur in various organs of fish as discrete aggregations of pigmented macrophages. the study presented herein investigates the quantitative modifications from normal anatomical condition, of interrenal gland (ig) and kidney ma in six treatment groups of adult rainbow trout submitted to either specific or aspecific immune stimulation and subsequently challenged with yersinia ruckeri. routinely stained tissue sections from both ig and kidney were analysed. the percentage ... | 2014 | 24719776 |
| establishment and characterization of a rainbow trout heart endothelial cell line with susceptibility to viral hemorrhagic septicemia virus (vhsv). | in the current work, we have established and characterized a novel cell line from rainbow trout (oncorhynchus mykiss). the cell line, designated as rth (rainbow trout heart), was obtained by immortalizing heart cells with recombinant retroviruses that transduced polyoma middle t antigen. this is the first time such a strategy is used to obtain an immortalized fish cell line. the cells showed an endothelial-like morphology and characteristics, constitutively transcribing collagen, selectin and vc ... | 2014 | 24698994 |
| short-term evaluation of visible implant alpha tags in juveniles of three fish species under laboratory conditions. | visible implant alpha (vi alpha) tag-induced changes in mortality and condition, as well as tag retention and readability, were examined during a 4-week period for juveniles of three fish species: tiger muskellunge esox masquinongy × esox lucius (91 ± 7 mm total length, l(t), mean ± s.d.), snake river cutthroat trout oncorhynchus clarki behnkei (84 ± 8 mm) and rainbow trout oncorhynchus mykiss (85 ± 5 mm). mortality and condition did not differ between tagged fish and control fish for any specie ... | 2014 | 24689672 |
| estimating the prevalence of renibacterium salmoninarum-infected salmonid production sites. | 2015 | 24602096 | |
| 3d visualization of the initial yersinia ruckeri infection route in rainbow trout (oncorhynchus mykiss) by optical projection tomography. | despite the fact that enteric redmouth disease (erm) in farmed rainbow trout is one of the most devastating disease problems, little is known about the initial route of infection and pathogenicity of the aetiological agent, yersinia ruckeri. in order to determine the initially infected organs, optical projection tomography (opt), a novel three-dimensional (3d) bio-imaging technique, was applied. opt not only enables the visualization of y. ruckeri on mucosal surfaces but also the 3d spatial dist ... | 2014 | 24586953 |
| food pellets as an effective delivery method for a dna vaccine against infectious pancreatic necrosis virus in rainbow trout (oncorhynchus mykiss, walbaum). | a dna vaccine based on the vp2 gene of infectious pancreatic necrosis virus (ipnv) was incorporated into feed to evaluate the effectiveness of this oral delivery method in rainbow trout. lyophilized alginate-plasmid complexes were added to feed dissolved in water and the mixture was then lyophilized again. we compared rainbow trout that were fed for 3 consecutive days with vaccine pellets with fish that received the empty plasmid or a commercial pellet. vp2 gene expression could be detected in t ... | 2014 | 24561129 |
| the effects of dietary immunogen on innate immune response, immune related genes expression and disease resistance of rainbow trout (oncorhynchus mykiss). | this study investigates the effects of prebiotic immunogen on lysozyme, tnfα and hsp70 gene expression in head kidney, humoral innate immune parameters and resistant to aeromonas hydrophila of rainbow trout. 120 healthy rainbow trout (81.65 ± 1.49 g) were distributed in six fiberglass tanks assigned to two groups fed control or diet supplemented with 2 g kg(-1) immunogen for 45 days. the results revealed that administration of immunogen significantly (p < 0.05) up regulated lysozyme and tnfα gen ... | 2014 | 24560683 |
| phenotypical and genotypical characterization of shewanella putrefaciens strains isolated from diseased freshwater fish. | between 2007 and 2012, a variety of disease outbreaks most often characterized by skin disorders were observed among different species of freshwater fish in poland. in most cases, the clinical signs included focally necrotized gills, necrotic skin lesions or ulcers. internally, haemorrhages, oedematous kidney and abnormal spleen enlargement were generally noted. the disorders were accompanied by increased mortality. most of the problems concerned cultured common carp cyprinus carpio l. and rainb ... | 2015 | 24552171 |
| evaluation of the immune response in rainbow trout fry, oncorhynchus mykiss (walbaum), after waterborne exposure to flavobacterium psychrophilum and/or hydrogen peroxide. | the immune response in rainbow trout fry against flavobacterium psychrophilum was elucidated using an immersion-based challenge with or without prior exposure to hydrogen peroxide (h2o2). samples were taken from the head kidney 4, 48, 125 and 192 h after immersion, and the regulation of several genes was examined. bacterial load was assessed based on the presence of 16s rrna and correlated with gene expression, and the levels of specific antibodies in the blood were measured 50 days post-infecti ... | 2015 | 24547972 |
| a new potassium ion current induced by stimulation of m2 cholinoreceptors in fish atrial myocytes. | a novel potassium ion current induced by muscarinic stimulation (ikach2) is characterized in atrial cardiomyocytes of teleost fishes (crucian carp, carassius carassius; rainbow trout, oncorhynchus mykiss) by means of the whole-cell patch-clamp technique. the current is elicited in atrial, but not ventricular, cells by application of carbamylcholine (cch) in moderate to high concentrations (10(-7)-10(-4) mol l(-1)). it can be distinguished from the classic ikach, activated by the βγ-subunit of th ... | 2014 | 24526726 |
| grouping facilitates avoidance of parasites by fish. | parasite distribution is often highly heterogeneous, and intensity of infection depends, among other things, on how well hosts can avoid areas with a high concentration of parasites. we studied the role of fish behaviour in avoiding microhabitats with a high infection risk using oncorhynchus mykiss and cercariae of diplostomum pseudospathaceum as a model. spatial distribution of parasites in experimental tanks was highly heterogeneous. we hypothesized that fish in groups are better at recognizin ... | 2013 | 24499549 |
| infection of sea lamprey with an unusual strain of aeromonas salmonicida. | the invasion of the laurentian great lakes by the fish-parasitic sea lamprey has led to catastrophic consequences, including the potential introduction of fish pathogens. aeromonas salmonicida is a bacterial fish pathogen that causes devastating losses worldwide. currently, there are five accepted subspecies of aeromonas salmonicida: a. salmonicida subsp. salmonicida, masoucida, smithia, achromogenes, and pectinolytica. we discuss the discovery of an isolate of a. salmonicida that is pathogenic ... | 2014 | 24484501 |
| a species comparison of 17-α-ethynylestradiol uptake and tissue-specific distribution in six teleost fish. | differences exist among fish species in their sensitivity to endocrine disruptors such as 17-α-ethynylestradiol (ee2). we examined whether there were corresponding differences in ee2 uptake rates and short-term internal distribution patterns. six freshwater species: japanese ricefish (medaka, oryzias latipes), goldfish (carassius auratus), zebrafish (danio rerio), fathead minnow (pimephales promelas), atlantic killifish (fundulus heteroclitus) and rainbow trout (oncorhynchus mykiss) were exposed ... | 2014 | 24473066 |
| effect of montanide™ ims 1312 vg adjuvant on efficacy of yersinia ruckeri vaccine in rainbow trout (oncorhynchus mykiss). | the efficacy of immersion vaccination yersinia ruckeri bacterin containing montanide™ ims 1312 vg was evaluated in 100-120 g rainbow trout against yersiniosis. healthy fish were vaccinated by immersion vaccination with inactivated whole cells (1 × 10(8) cells/ml) of a virulent strain of y. ruckeri biotype i with and without montanide (1:1; montanide/antigen) for 2 min at 12-14 °c. control group was immersed in sterile pbs. leukocyte counts, serum lysozyme assay, alternative hemolytic complement ... | 2014 | 24444826 |
| sequencing and characterization of the anadromous steelhead (oncorhynchus mykiss) transcriptome. | identifying the traits that differ between hatchery and wild fish may allow for pragmatic changes to hatchery practice. to meet those ends, we sequenced, assembled, and characterized the anadromous steelhead (oncorhynchus mykiss) transcriptome. using the illumina sequencing platform, we sequenced nearly 41million 76-mer reads representing 3.1 gbp of steelhead transcriptome. upon final assembly, this sequence data yielded 86,402 transcript scaffolds, of which, 66,530 (77%) displayed homology to p ... | 2014 | 24440488 |
| staphylococcus warneri, a resident skin commensal of rainbow trout (oncorhynchus mykiss) with pathobiont characteristics. | commensal microorganisms live in association with the mucosal surfaces of all vertebrates. the skin of teleost fish is known to harbor commensals. in this study we report for the first time the presence of an intracellular gram positive bacteria, staphylococcus warneri that resides in the skin epidermis of rainbow trout (oncorhynchus mykiss). s. warneri was isolated from healthy hatchery trout skin epithelial cells. in situ hybridization confirmed the intracellular nature of the bacterium. skin ... | 2014 | 24438987 |
| inhibition of β-n-acetylglucosaminidase by acetamide affects sperm motility and fertilization success of rainbow trout (oncorhynchus mykiss) and siberian sturgeon (acipenser baerii). | β-n-acetylglucosaminidase (β-nagase) is an enzyme found in the sperm acrosome of numerous animal species including fish. fish spermatozoa differ in their morphology including acrosome or acrosomeless aquasperm in chondrostean (e.g., sturgeon) and teleostean (e.g., rainbow trout). it has been shown that β-nagase exists with high activity in both eggs and sperm of these species. the present study shows the potency of β-nagase in fertilization. in rainbow trout, increase in sperm motility parameter ... | 2014 | 24433780 |
| salmonid alphavirus replication in mosquito cells: towards a novel vaccine production system. | salmonid alphavirus (sav) causes pancreas disease and sleeping disease in atlantic salmon (salmo salar) and rainbow trout (oncorhynchus mykiss) and confers a major burden to the aquaculture industry. a commercial inactivated whole virus vaccine propagated in a salmon cell line at low temperature provides effective protection against sav infections. alphaviruses (family togaviridae) are generally transmitted between vertebrate hosts via blood-sucking arthropod vectors, typically mosquitoes. sav i ... | 2014 | 24418177 |
| the effects of benzo[a]pyrene on leucocyte distribution and antibody response in rainbow trout (oncorhynchus mykiss). | polycyclic aromatic hydrocarbons (pahs) are a group of compounds with immunotoxic and carcinogenic potential that may pose a threat to fish populations. this study aims to utilize a newly developed fish immunotoxicology model to determine the immune tissue/cell population level effects of pahs on rainbow trout, using benzo[a]pyrene (bap) as a representative immunotoxic pah. intraperitoneal injection of 25 or 100mg/kg bap resulted in sustained exposure as indicated by biliary fluorescence at bap ... | 2014 | 24398112 |
| association of a specific major histocompatibility complex class iiβ single nucleotide polymorphism with resistance to lactococcosis in rainbow trout, oncorhynchus mykiss (walbaum). | major histocompatibility complex (mhc) loci encode glycoproteins that bind to foreign peptides and initiate immune responses through their interaction with t cells. mhc class ii molecules are heterodimers consisting of α and β chains encoded by extremely variable genes; variation in exon 2 is responsible for the majority of observed polymorphisms, mostly concentrated in the codons specifying the peptide-binding region. lactococcus garvieae is the causative agent of lactococcosis, a warm-water ba ... | 2015 | 24397583 |
| [prokaryotic expression and immunogenicity analysis of glycoprotein from infectious hematopoietic necrosis virus]. | in order to detect infectious hematopoietic necrosis virus with immunological methods, the surface glycoprotein of a recent ihnv-sn isolated from farmed rainbow trout ( oncorhynchus mykiss ) in china was amplified and cloned into pet27b(+) vector (designated as pet27b-g ). the expression of recombinant plasmid pet27b-g in e. coli bl21(de3) was induced and determined by sds-page analysis. the predicted molecular weight of glycoprotein protein was approximately 55 kd and was confirmed in this stud ... | 2013 | 24386842 |
| expression profile of ribosomal protein l10a throughout gonadal development in rainbow trout (oncorhynchus mykiss). | ribosomal protein l10a (rpl10a) has been previously established as a stimulator during the early stages of ovarian development in both the banana prawn and the fruit fly. in order to develop a greater understanding of the role of this protein in vertebrates, the present study aimed to characterize the expression profile of rpl10a during gonadal development in fish. it was determined that the expression of rpl10a within genital ridges increased during embryonic development. although rpl10a expres ... | 2014 | 24385218 |
| enhanced aeromonas salmonicida bacterin uptake and side effects caused by low frequency sonophoresis in rainbow trout (oncorhynchus mykiss). | low frequency sonophoresis (lfs) has been recognized as one of the most advanced technologies in transdermal delivery of substances, due to the modification of the stratum corneum lipid bilayer, in focal skin applications in mammals. based on these findings, lfs has been suggested as a potential technology to be used for enhancement in immersion fish vaccination. in contrast to mammals where lfs is applied to discrete regions of the skin, in fish the whole individual needs to be exposed for prac ... | 2014 | 24378683 |
| modulation of genes related to the recruitment of immune cells in the digestive tract of trout experimentally infected with infectious pancreatic necrosis virus (ipnv) or orally vaccinated. | there are still many details of how intestinal immunity is regulated that remain unsolved in teleost. although leukocytes are present all along the digestive tract, most immunological studies have focused on the posterior segments and the importance of each gut segment in terms of immunity has barely been addressed. in the current work, we have studied the regulation of several immune genes along five segments of the rainbow trout (oncorhynchus mykiss) digestive tract, comparing the effects obse ... | 2014 | 24370535 |
| ccr7 is mainly expressed in teleost gills, where it defines an igd+igm- b lymphocyte subset. | chemokine receptor ccr7, the receptor for both ccl19 and ccl21 chemokines, regulates the recruitment and clustering of circulating leukocytes to secondary lymphoid tissues, such as lymph nodes and peyer's patches. even though teleost fish do not have either of these secondary lymphoid structures, we have recently reported a homolog to ccr7 in rainbow trout (oncorhynchus mykiss). in the present work, we have studied the distribution of leukocytes bearing extracellular ccr7 in naive adult tissues ... | 2014 | 24353268 |
| molecular docking: a potential tool to aid ecotoxicity testing in environmental risk assessment of pharmaceuticals. | a cocktail of human pharmaceuticals pollute aquatic environments and there is considerable scientific uncertainty about the effects that this may have on aquatic organisms. human drug target proteins can be highly conserved in non target species suggesting that similar modes of action (moa) may occur. the aim of this work was to explore whether molecular docking offers a potential tool to predict the effects of pharmaceutical compounds on non target organisms. three highly prescribed drugs, dicl ... | 2013 | 24344392 |
| rapid method for the measurement of circulating thyroid hormones in low volumes of teleost fish plasma by lc-esi/ms/ms. | thyroid hormones are critical regulators of normal development and physiological functioning in all vertebrates. radioimmunoassay (ria) approaches have been the method of choice for measuring circulating levels of thyroid hormones in vertebrates. while sensitive, ria-based approaches only allow for a single analyte measurement per assay, can lack concordance across platforms and laboratories, and can be prone to analytical interferences especially when used with fish plasma. ongoing advances in ... | 2014 | 24343452 |
| transfer of serum and cells from yersinia ruckeri vaccinated doubled-haploid hot creek rainbow trout into outcross f1 progeny elucidates mechanisms of vaccine-induced protection. | yersinia ruckeri is a well-established bacterial pathogen for many salmonid species, against which a formalin-killed bacterin vaccine has been effective in reducing disease outbreaks. previous studies have reported conflicting results about the protective value of the systemic humoral response to y. ruckeri vaccination. here we directly demonstrate that plasma contains the long-term protective component elicited by both immersion and intraperitoneal injection vaccination of rainbow trout. a tota ... | 2014 | 24342572 |
| acute mortality, bacterial load, and pathology of select lines of adult rainbow trout challenged with weissella sp. nc36. | a challenge for improving disease resistance in fish through genetics is to understand specificity of resistance and whether selection for one pathogen alters the response to unrelated pathogenic microorganisms. adult rainbow trout oncorhynchus mykiss that had been bred for differential susceptibility to flavobacterium psychrophilum, the causative agent of bacterial cold water disease (bcwd) and designated ars-fp-r (resistant), ars-fp-s (susceptible), and ars-fp-c (control line), as well as a po ... | 2013 | 24341763 |
| virulence of viral hemorrhagic septicemia virus (vhsv) genotypes ia, iva, ivb, and ivc in five fish species. | the susceptibility of yellow perch perca flavescens, rainbow trout oncorhynchus mykiss, chinook salmon o. tshawytscha, koi cyprinus carpio koi, and pacific herring clupea pallasii to 4 strains of viral hemorrhagic septicemia virus (vhsv) was assessed. fish were challenged via intraperitoneal injection with high (1 × 106 plaque-forming units, pfu) and low (1 × 103 pfu) doses of a european strain (genotype ia), and north american strains from the west coast (genotype iva), great lakes (genotype iv ... | 2013 | 24334352 |
| fate of tetracapsuloides bryosalmonae (myxozoa) after infection of brown trout salmo trutta and rainbow trout oncorhynchus mykiss. | tetracapsuloides bryosalmonae (myxozoa) is the causative agent of proliferative kidney disease in salmonids. we assessed differences in intensity of t. bryosalmonae infection between brown trout salmo trutta and rainbow trout oncorhynchus mykiss from the clinical phase of infection onwards. specific pathogen-free fish were exposed to t. bryosalmonae spores under controlled laboratory conditions and sampled at 6, 8, 10, 12, 14, and 17 wk post exposure (wpe), and the transmission of t. bryosalmona ... | 2013 | 24270019 |
| social stress modulates the cortisol response to an acute stressor in rainbow trout (oncorhynchus mykiss). | in rainbow trout (oncorhynchus mykiss) of subordinate social status, circulating cortisol concentrations were elevated under resting conditions but the plasma cortisol and glucose responses to an acute stressor (confinement in a net) were attenuated relative to those of dominant trout. an in vitro head kidney preparation, and analysis of the expression of key genes in the stress axis prior to and following confinement in a net were then used to examine the mechanisms underlying suppression of th ... | 2014 | 24269985 |
| transcriptomic responses in rainbow trout gills upon infection with viral hemorrhagic septicemia virus (vhsv). | it has been previously demonstrated that even though the fin bases constitute the main portal of entry of viral hemorrhagic septicemia virus (vhsv) in rainbow trout (oncorhynchus mykiss), an important number of chemokine genes are up-regulated in the gills upon bath exposure to the virus. because chemokines mediate the recruitment of leukocytes through the action of specific chemokine receptors, in the current study, we have studied the transcription of several immune genes in response to a vhsv ... | 2014 | 24269609 |
| detection of qtl in rainbow trout affecting survival when challenged with flavobacterium psychrophilum. | bacterial cold water disease (bcwd) causes significant economic loss in salmonid aquaculture. we previously detected genetic variation in survival following challenge with flavobacterium psychrophilum (fp), the causative agent of bcwd in rainbow trout (oncorhynchus mykiss). a family-based selection program to improve resistance was initiated in 2005 at the usda national center for cool and cold water aquaculture. select crosses were made in 2007 and 2009 to evaluate family-based disease survival ... | 2014 | 24241385 |
| characterization of the interleukin 1 receptor-associated kinase 4 (irak4)-encoding gene in salmonid fish: the functional copy is rearranged in oncorhynchus mykiss and that factor can impair tlr signaling in mammalian cells. | the interleukin 1 receptor-associated kinase 4 (irak4) is an essential factor for tlr-mediated activation of the host's immune functions subsequent to pathogen contact. we have characterized the respective cdna and gene sequences from three salmonid species, salmon, rainbow trout and maraena whitefish. the gene from salmon is structured into eleven exons, as is the mammalian homologue, while exons have been fused in the genes from the two other salmonid species. rainbow trout expresses also a ps ... | 2014 | 24239597 |
| comparative study of acetylcholine synthesis in organs of freshwater teleosts. | the activity of acetylcholine (ach) synthesizing enzyme, choline acetyltransferase (chat), and the content of ach were determined in the brain, heart, red trunk muscle and midgut of 12 freshwater teleost species belonging to eight families: carp (cyprinus carpio), tench (tinca tinca), silver carp (hypophthalmichtys molitrix), bighead (hypophthalmichtys nobilis), wels (silurus glanis), cat fish (ictalurus nebulosus), eel (anguilla anguilla), rainbow trout (oncorhynchus mykiss), pike (esox lucius) ... | 1991 | 24214667 |
| interrelationships between gill chloride cell morphology and calcium uptake in freshwater teleosts. | the involvement of the freshwater fish gill chloride cells (ccs) in trans-branchial calcium uptake (jinca(2+)) was investigated. this was accomplished by assessing the interspecific relationships between the apical surface area of ccs exposed to the external environment and jinca(2+). three species of freshwater teleosts, the rainbow trout (oncorhynchus mykiss), the american eel (anguilla rostrata) and the brown bullhead catfish (ictalurus nebulosus), were used. chronic (ten-day) treatment with ... | 1992 | 24214330 |
| erythrocyte fractionation by velocity sedimentation and discontinuous density gradient centrifugation. | investigation of development, maturation and senescence in fish red blood cells depends on effective separation methods. preliminary to initiation of studies of this type the erythrocytes of goldfish,carassius auratus, and rainbow trout,oncorhynchus mykiss, were fractionated by velocity sedimentation at unit gravity or discontinuous percoll density gradient centrifugation in an attempt to separate and characterize enriched populations of juvenile and mature cells. cytomorphological variables wer ... | 1991 | 24213719 |
| ecvig, a novel grouper immune-gene associated with antiviral activity against nnv infection. | vhsv-induced genes (vigs) were first identified in rainbow trout (oncorhynchus mykiss) and subsequently isolated in a variety of fish. recent studies have shown that most vigs have immunological functions against pathogenic infections. however, most research has focused on vig1, such that our present understanding of these genes in other fish species remains limited. this study isolated a homologue of the uncharacterized o. mykiss vig-b319 (ecvig) from orange-spotted grouper (epinephelus coioide ... | 2014 | 24211340 |
| the effect of various stresses, corticosteroids and adrenergic agents on phagocytosis in the rainbow trout oncorhynchus mykiss. | the effect of acute and chronic stress on the phagocytic activity of putative macrophages from the rainbow trout. oncorhynchus mykiss has been assessed, using an in vitro phagocytic index, in which the average number of engulfed yeast cells in a population of phagocytes is determined. an injection stress given under light anaesthesia, or a longer noise stress combined with confinement, both significantly reduced, within 3 h, the level of phagocytic activity of macrophages from the spleen and pro ... | 1994 | 24203269 |
| insulin stimulates hepatic lipogenesis in rainbow trout, oncorhynchus mykiss. | the effects of the pancreatic hormones, insulin and glucagon, on rates of lipid biosynthesis in liver removed from rainbow trout, oncorhynchus mykiss, were evaluated in vitro. livers were removed from animals fasted for 30-36h, cut into ca. 1 mm(3) pieces, and incubated in the presence of various concentrations of salmon insulin (sins), bovine insulin (bins), or a combination of bins and bovine/porcine glucagon (glu). lipid synthesis was evaluated by total lipid concentration, (3)h2o incorporati ... | 1993 | 24202502 |
| development of a protein binding assay for teleost insulin-like growth factor (igf)-like: relationships between growth hormone (gh) and igf-like in the blood of rainbow trout (oncorhynchus mykiss). | using rainbow trout plasma protein (igf-bp) which specifically binds human insulin-like growth factor (igf) (niu and le bail 1993), we have developed an assay to measure plasma igf-like levels in different teleost species. before the assay and to prevent interference by igf-bp, igf-like was extracted from all samples, using sp sephadex c-25 in acidic conditions. after this treatment, contamination of the igf fraction by igf-bp which was estimated by binding assay, was approximately 5%, and was n ... | 1993 | 24202498 |
| phylogenetic analysis and positive-selection site detecting of vascular endothelial growth factor family in vertebrates. | vascular endothelial growth factor (vegf), known to play an important role in vascular homeostasis, vascular integrity and angiogenesis, is little known about the evolutionary relationship of its five members especially the role of gene duplication and natural selection in the evolution of the vegf family. in this study, seventy-five full-length cdna sequences from 33 vertebrate species were extracted from the ncbi's genbank, uniprot protein database and the ensembl database. by phylogenetic ana ... | 2014 | 24200960 |
| frequency analyses of active nors in nuclei of artificially induced triploid fishes. | the correspondence between increased numbers of both chromosomal and nuclear nors and artificially induced triploidy in three fish species (rainbow trout, oncorhynchus mykiss; common carp, cyprinus carpio; and tench, tinea tinea) has been confirmed by cma3 fluorescence and ag-staining. the frequencies of cell nuclei with one, two and three active nors, as revealed by ag-staining, has been analyzed statistically to find the minimum cell number which verifies the increased ploidy level. a minimum ... | 1992 | 24197230 |
| endogenous antioxidant systems of two teleost fish, the rainbow trout and the black bullhead, and the effect of age. | antioxidant enzyme activities and glutathione status were determined in different tissues of two teleost species, the rainbow trout (oncorhynchus mykiss) and the black bullhead (ameiurus melas) to establish whether age-related changes exist between mature and immature individuals. glutathione reductase and superoxide dismutase activities were significantly lower in hepatic and extrahepatic tissues of 3+ year than 1+ year trout and bullheads. activities of glutathione peroxidase, catalase and glu ... | 1996 | 24194254 |
| immunochemical relationships of cytochrome p4503a-like proteins in teleost fish. | multiple p450 proteins have been purified from several teleost species, including rainbow trout (oncorhynchus mykiss), scup (stenotomus chrysops) and atlantic cod (gadus morhua). identity, relationships and/or functions have been established in these fish species for the cytochrome p4501 as. information about the structure, function, regulation and relationships of other piscine cytochrome p450 (cyp) proteins is sparse. in the present study we have focused on constitutively expressed cyp forms, ... | 1996 | 24194251 |
| effect of growth hormone on muscle protein synthesis in rainbow trout (oncorhynchus mykiss) and atlantic salmon (salmo salar). | this paper reports on the effect of administration of mammalian growth hormone (gh) on muscle protein synthesis as measured in white muscle using the phenylalanine flooding technique. the effect of exogenous gh was compared with that of insulin and prolactin, and with endogenous gh.the rate of protein synthesis in white muscle of rainbow trout 6 h after the injection of bovine gh or bovine insulin was twice (2.6 and 2.9% d(-1)) that of the control saline-injected fish (1.2% d(-1)). a metabolic e ... | 1996 | 24193988 |
| genotype-specific vs. cross-reactive host immunity against a macroparasite. | vertebrate hosts often defend themselves against several co-infecting parasite genotypes simultaneously. this has important implications for the ecological dynamics and the evolution of host defence systems and parasite strategies. for example, it can drive the specificity of the adaptive immune system towards high genotype-specificity or cross-reactivity against several parasite genotypes depending on the sequence and probability of re-infections. however, to date, there is very little evidence ... | 2013 | 24167622 |
| biochemical and molecular heterogeneity among isolates of yersinia ruckeri from rainbow trout (oncorhynchus mykiss, walbaum) in north west germany. | enteric redmouth disease (erm), caused by yersinia ruckeri, is one of the most important infectious diseases in rainbow trout (oncorhynchus mykiss) aquaculture in europe. more recently, non-motile vaccine resistant isolates appear to have evolved and are causing disease problems throughout europe, including germany. the aim of this study was to analyse the variation of biochemical and molecular characteristics of y. ruckeri isolates collected in north west germany as a basis for strain different ... | 2013 | 24144165 |
| pharmacokinetics and residue depletion of erythromycin in rainbow trout oncorhynchus mykiss (walbaum). | erythromycin (ery) is a drug active against gram-positive bacteria such as lactococcus garvieae, a pathogen responsible for an important disease that may cause a substantial decrease in rainbow trout oncorhynchus mykiss (walbaum) production, the species of fish most commonly produced in italy. in the literature, studies on the kinetics behaviour of ery in fish are limited. therefore, the aim of the present study was to evaluate the pharmacokinetics of ery in rainbow trout after a single oral tre ... | 2013 | 24118016 |
| hexamitiasis leads to lower metabolic rates in rainbow trout oncorhynchus mykiss (walbaum) juveniles. | this study assessed the effects of hexamita salmonis (moore) on metabolism of rainbow trout oncorhynchus mykiss (walbaum) and its effect on the host's susceptibility to infectious pancreatic necrosis virus (ipnv) after antiparasitic treatment. rainbow trout naturally infected with h. salmonis were treated with 10 mg metronidazole kg fish(-1) per day, and their physiological recovery was assessed through measuring resting metabolism on the 7th, 14th, 21st and 28th day after treatment. in addition ... | 2014 | 24117757 |
| oil and oil dispersant do not cause synergistic toxicity to fish embryos. | atlantic herring (clupea harengus) embryos were exposed to water accommodated fractions (wafs; oil dissolved in water) and chemically enhanced water accommodated fractions (cewafs; oil dispersed in water with corexit 9500a) of medium south american (mesa) crude oil. the cewaf was approximately 100-fold more toxic than waf based on nominal loadings of test solutions (% v/v). in contrast, the ratio of waf and cewaf toxicity expressed as measured oil concentrations approximated 1.0, indicating that ... | 2014 | 24115182 |
| universal reverse-transcriptase real-time pcr for infectious hematopoietic necrosis virus (ihnv). | infectious hematopoietic necrosis virus (ihnv) is an acute pathogen of salmonid fishes in north america, europe and asia and is reportable to the world organization for animal health (oie). phylogenetic analysis has identified 5 major virus genogroups of ihnv worldwide, designated u, m, l, e and j; multiple subtypes also exist within those genogroups. here, we report the development and validation of a universal ihnv reverse-transcriptase real-time pcr (rt-rpcr) assay targeting the ihnv nucleoca ... | 2013 | 24113244 |
| green tea (camellia sinensis) administration induces expression of immune relevant genes and biochemical parameters in rainbow trout (oncorhynchus mykiss). | present study elucidates the efficacy of green tea (camellia sinensis) on growth performance, immune and antioxidant systems and cytokine gene expression in rainbow trout tissues. green tea was supplemented at 20, 100, and 500 mg kg(-1) diet and fed to fish (average weight: 23.5 g) for 35 days. no remarkable changes in growth performance were observed among all test groups. lower lipid peroxidation product and higher superoxide dismutase activity were noted in fish received the medium dose of gr ... | 2013 | 24096104 |
| flavobacterium tructae sp. nov. and flavobacterium piscis sp. nov., isolated from farmed rainbow trout (oncorhynchus mykiss). | four gram-staining-negative, catalase- and oxidase-positive, pale-orange pigmented bacterial strains (435-08(t), 47b-3-09, 412r-09(t) and 60b-3-09) were isolated from diseased rainbow trout. analysis of their 16s rrna gene sequences suggested their adscription to the genus flavobacterium. strains formed two phylogenetic groups represented by strains 435-08(t) and 47b-3-09 (group a), and strains 412r-09(t) and 60b-3-09 (group b) displaying 16s rrna sequence similarities greater than 99.8-99.9% wi ... | 2014 | 24065772 |
| rainbow trout peptidoglycan recognition protein has an anti-inflammatory function in liver cells. | peptidoglycan recognition proteins (pgrps) are innate immune molecules that are structurally conserved through evolution in both invertebrate and vertebrate animals. pgrps exert diverse host-defense functions both through direct antibacterial activity and through indirect effects, including the induction of antimicrobial peptides and the modulation of inflammation and immune responses. in this study, we identified the gene encoding a long form of pgrp (ompgrp-l1) from the rainbow trout, oncorhyn ... | 2013 | 24056276 |
| cloning, molecular characterization, and expression analysis of the signal transducer and activator of transcription 3 (stat₃) gene from grass carp (ctenopharyngodon idellus). | signal transducer and activator of transcription 3 (stat₃) binds to janus kinase 2 (jak₂) to initiate the jak₂/stat₃ signal transduction pathway, which plays an important role in cancer cell proliferation, immune regulation, reproduction, lipid metabolism, and other physiological processes of the organism. in this study, the cdna sequence of the stat₃ gene from grass carp was cloned using race (rapid-amplification of cdna ends). twelve characteristics of the stat₃ gene and its encoded protein se ... | 2013 | 24055509 |
| expression of immune relevant genes in rainbow trout following exposure to live anisakis simplex larvae. | basic immune response mechanisms in vertebrates against helminths are still poorly understood. fish-nematode models may prove valuable for elucidation of this question. in this study we orally challenged rainbow trout (oncorhynchus mykiss) with larvae of anisakis simplex (nematoda: anisakidae) and subsequently investigated the expression of 18 immune relevant genes in spleen and liver 1, 4 and 8days post infection (d.p.i.). gene expression data were analysed with regard to the infection status o ... | 2013 | 24051344 |
| species- and chamber-specific responses of 12 kda fk506-binding protein to temperature in fish heart. | the sarcoplasmic reticulum (sr) ca(2+) release channel or ryanodine receptor (ryr) of the vertebrate heart is regulated by the fk506-binding proteins, fkbp12 and fkbp12.6. this study examines whether temperature-related changes in the sr function of fish hearts are associated with changes in fkbp12 expression. for this purpose, a polyclonal antibody against trout fkbp12 was used to compare fkpb12 expression in cold-acclimated (4 °c, ca) and warm-acclimated (18 °c, wa) rainbow trout (oncorhynchus ... | 2014 | 24048915 |
| sequence analysis of infectious pancreatic necrosis virus isolated from iranian reared rainbow trout (oncorhynchus mykiss) in 2012. | infectious pancreatic necrosis virus (ipnv) is the causal agent of a highly contagious disease that affects many species of fish and shellfish. this virus causes economically significant diseases of farmed rainbow trout, oncorhynchus mykiss (walbaum), in iran, which is often associated with the transmission of pathogens from european resources. in this study, moribund rainbow trout fry samples were collected during an outbreak of ipnv in three different fish farms in north and west provinces of ... | 2013 | 24048887 |
| the immunological effects of oil sands surface waters and naphthenic acids on rainbow trout (oncorhynchus mykiss). | there is concern surrounding the immunotoxic potential of naphthenic acids (nas), a major organic constituent in waters influenced by oil sands contamination. to assess the immunological response to nas, rainbow trout (oncorhynchus mykiss) waterborne exposures were conducted with oil sands-influenced waters, nas extracted and purified from oil sands tailings waters, and benzo[a]pyrene (bap) as a positive control. after a 7d exposure, blood, spleen, head kidney, and gill samples were removed from ... | 2013 | 24036435 |
| cloning and characterization of a novel oocyte-specific gene encoding an f-box protein in rainbow trout (oncorhynchus mykiss). | oocyte-specific genes play critical roles in oogenesis, folliculogenesis and early embryonic development. the objectives of this study were to characterize the expression of a novel oocyte-specific gene encoding an f-box protein during ovarian development in rainbow trout, and identify its potential interacting partners in rainbow trout oocytes. | 2013 | 24007267 |
| characterization and expression of gamma-interferon-inducible lysosomal thiol reductase (gilt) gene in rainbow trout (oncorhynchus mykiss) with implications for gilt in innate immune response. | the interferon-γ-inducible lysosomal thiol reductase (gilt) has been demonstrated to play an important role in the processing and presentation of mhc class ii-restricted antigen (ag) by catalyzing disulfide bond reduction. in this study, a rainbow trout cdna (designated as rgilt) was cloned and identified from oncorhynchus mykiss. the open reading frame of rgilt consists of 759 bases encoding a protein of 253 amino acids with an estimated molecular mass of 28.23 kda and a theoretical isoelectric ... | 2013 | 24006043 |
| molecular epidemiology of flavobacterium psychrophilum from swiss fish farms. | bacterial cold water disease (bcwd) and rainbow trout fry syndrome (rtfs) caused by flavobacterium psychrophilum are 2 of the major diseases causing high fish mortality in salmonid fish farms. the molecular epidemiology of f. psychrophilum is still largely unknown. multilocus sequence typing (mlst) has been previously used for this pathogen and underscored a correlation between clonal complexes and host fish species. here we used mlst to study the relationships among 112 f. psychrophilum isolate ... | 2013 | 23999704 |
| characterization of oncorhynchus mykiss 5-hydroxyisourate hydrolase/transthyretin superfamily: evolutionary and functional analyses. | teleosts have highly diverged genomes that resulted from whole genome duplication, which leads to an extensive diversity of paralogous genes. transthyretin (ttr), an extracellular thyroid hormone (th) binding protein, is thought to have evolved from an ancestral 5-hydroxyisourate hydrolase (hiuhase) by gene duplication at some stage of chordate evolution. to characterize the functions of proteins that arose from duplicated genes in teleosts, we investigated the phylogenetic relationship of teleo ... | 2013 | 23994290 |
| enhanced efficacy of an attenuated flavobacterium psychrophilum strain cultured under iron-limited conditions. | an attenuated strain of flavobacterium psychrophilum (csf259-93b.17) has shown potential as a vaccine for prevention of bacterial coldwater disease (bcwd) in rainbow trout, oncorhynchus mykiss (walbaum). because bcwd outbreaks can result in high mortality in other salmonid species, specifically coho salmon, oncorhynchus kisutch (walbaum), the live-attenuated strain was tested as a vaccine in this species. additionally, we hypothesized that culture of the vaccine strain under iron-limited conditi ... | 2013 | 23989039 |