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phylogeographical structure in mitochondrial dna of whitefly, bemisia tabaci gennadius (hemiptera: aleyrodidae) in southern india and southeast asia.south and southeast asia endure high vegetable production losses due to begomovirus diseases mainly transmitted by the insect vector, whitefly (bemisia tabaci). control over the spread of virus infection can be achieved through a better understanding of genetic diversity among b. tabaci. a total of 64 populations of b. tabaci collected from tamil nadu (india), vietnam, thailand, and indonesia were investigated based on mitochondrial cytochrome c oxidase i (coxi) sequences. populations from tamil ...201627159720
molecular characterization of vitellogenin and vitellogenin receptor of bemisia tabaci.vitellogenin (vg) plays vital role in oocytes and embryo development in insects. vg is synthesized in the fat body, moves through haemolymph and accumulates in oocytes. vitellogenin receptors (vgr) present on the surface of oocytes, are responsible for vg transportation from haemolymph to oocytes. here, we cloned and characterized these genes from bemisia tabaci asia1 (bta1) species. the cloned bta1vg and bta1vgr genes consisted of 6,330 and 5,430 bp long open reading frames, which encoded 2,109 ...201627159161
no cross-resistance between imidacloprid and pymetrozine in the brown planthopper: status and mechanisms.cross-resistance between insecticides, especially from different groups, can be extremely unpredictable, and it has been a serious concern in pest control. pymetrozine has been widely used to control nilaparvata lugens with the suspension of imidacloprid for the resistance, and n. lugens has showed obvious pymetrozine resistance in recent years. to investigate the possible cross-resistance between imidacloprid and pymetrozine is very important to avoid the adverse effects on resistance developme ...201627155488
the suitability of biotypes q and b of bemisia tabaci (gennadius) (hemiptera: aleyrodidae) at different nymphal instars as hosts for encarsia formosa gahan (hymenoptera: aphelinidae).encarsia formosa gahan (hymenoptera: aphelinidae) is a solitary endoparasitoid that is commercially reared and released for augmentative biological control of whiteflies infesting greenhouse crops. in most areas in china, the invasive and destructive whitefly bemisia tabaci (gennadius) (hemiptera: aleyrodidae) biotype q has replaced b. tabaci biotype b and has become dominant between the two. a better understanding of the suitability of different nymphal instars of b. tabaci biotypes q and b as ...201627114862
rna interference based approach to down regulate osmoregulators of whitefly (bemisia tabaci): potential technology for the control of whitefly.over the past decade rna interference (rnai) technology has emerged as a successful tool not only for functional genomics, but in planta expression of short interfering rnas (sirnas) that could offer great potential for insect pest management. the diet of insects feeding exclusively on phloem sieves contains water and sugars as main components, and the uptake of the liquid food greatly depends on the osmotic pressure within the insect body. based on this physiological mechanism, transgenic plant ...201627105353
leaf morphological characters can be a factor for intra-varietal preference of whitefly bemisia tabaci (hemiptera: aleyrodidae) among eggplant varieties.the sweetpotato whitefly, bemisia tabaci (hemiptera: aleyrodidae) meam1, is considered a serious pest of horticultural and many other crops. while eggplant (solanum melongena) is one of the most favored host plants, the whiteflies exhibit preferences among different varieties. we hypothesized that certain morphological leaf characteristics of different varieties, like leaf trichome density, trichome length, leaf lamina thickness and leaf color, may affect whitefly landing, feeding and ovipositio ...201627081849
differential tolerance capacity to unfavourable low and high temperatures between two invasive whiteflies.thermal response and tolerance to ambient temperature play important roles in determining the geographic distribution and seasonal abundance of insects. we examined the survival and performance, as well as expression of three heat shock protein related genes, of two species of invasive whiteflies, middle east-asia minor 1 (meam1) and mediterranean (med), of the bemisia tabaci species complex following exposure to a range of low and high temperatures. our data demonstrated that the med species wa ...201627080927
differential and synergistic functionality of acylsugars in suppressing oviposition by insect herbivores.acylsugars are secondary metabolites exuded from type iv glandular trichomes that provide broad-spectrum insect suppression for solanum pennellii correll, a wild relative of cultivated tomato. acylsugars produced by different s. pennellii accessions vary by sugar moieties (glucose or sucrose) and fatty acid side chains (lengths and branching patterns). our objective was to determine which acylsugar compositions more effectively suppressed oviposition of the whitefly bemisia tabaci (gennadius) (m ...201627065236
biosafety of an entomopathogenic fungus isaria fumosorosea in an acute dermal test in rabbits.isaria fumosorosea (formerly paecilomyces fumosoroseus) is an entomopathogenic fungus that has been proposed as a low risk environmental alternative to the use of chemical insecticides to control agricultural pests and disease vectors. although there are some mycoinsecticides already being marketed in many countries, not all their biosafety protocols have been published. the acute dermal toxicity test in an animal model is one in a series of biosafety protocols that must be developed, in order t ...201727055483
whiteflies stabilize their take-off with closed wings.the transition from ground to air in flying animals is often assisted by the legs pushing against the ground as the wings start to flap. here, we show that when tiny whiteflies (bemisia tabaci, body length ca. 1 mm) perform take-off jumps with closed wings, the abrupt push against the ground sends the insect into the air rotating forward in the sagittal (pitch) plane. however, in the air, b. tabaci can recover from this rotation remarkably fast (less than 11 ms), even before spreading its wings ...201627045098
use of posttranscription gene silencing in squash to induce resistance against the egyptian isolate of the squash leaf curl virus.squash leaf curl virus (sqlcv) is a bipartite begomovirus affecting squash plants. it is transmitted by whitefly bemisia tabaci biotype b causing severe leaf curling, vein banding, and molting ending by stunting. in this study full-length genomic clone of sqlcv egyptian isolated and posttranscriptional gene silencing (ptgs) has been induced to develop virus resistance. the noubaria sqlcv has more than 95% homology with jordon, israel, lebanon, palestine, and cairo isolates. two genes fragment fr ...201627034922
complete nucleotide sequences of a new bipartite begomovirus from malvastrum sp. plants with bright yellow mosaic symptoms in south texas.two isolates of a novel bipartite begomovirus, tentatively named malvastrum bright yellow mosaic virus (mabymv), were molecularly characterized from naturally infected plants of the genus malvastrum showing bright yellow mosaic disease symptoms in south texas. six complete dna-a and five dna-b genome sequences of mabymv obtained from the isolates ranged in length from 2,608 to 2,609 nucleotides (nt) and 2,578 to 2,605 nt, respectively. both genome segments shared a 178- to 180-nt common region. ...201627016928
effects of host plant factors on the bacterial communities associated with two whitefly sibling species.although discrepancy in the specific traits and ecological characteristics of bemisia tabaci between species are partially attributed to the b. tabaci-associated bacteria, the factors that affect the diversity of b. tabaci-associated bacteria are not well-understood. we used the metagenomic approach to characterize the b. tabaci-associated bacterial community because the approach is an effective tool to identify the bacteria.201627008327
further insight into reproductive incompatibility between putative cryptic species of the bemisia tabaci whitefly complex.the whitefly bemisia tabaci (gennadius) (hemiptera: aleyrodidae), with its global distribution and extensive genetic diversity, is now known to be a complex of over 35 cryptic species. however, a satisfactory resolution of the systematics of this species complex is yet to be achieved. here, we designed experiments to examine reproductive compatibility among species with different levels of mitochondrial cytochrome oxidase i (mtcoi) divergence. the data show that putative species with mtcoi diver ...201627001484
infectious clones of the crinivirus cucurbit chlorotic yellows virus are competent for plant systemic infection and vector transmission.cucurbit chlorotic yellows virus (ccyv), a recently identified bipartite crinivirus, causes economic losses in cucurbit plants. ccyv is naturally transmitted only by whitefly bemisia tabaci. here we constructed full-length cdna clones of ccyv (rna1 and rna2) fused to the t7 rna polymerase promoter and the cauliflower mosaic virus 35s promoter. ccyv replicated and accumulated efficiently in cucumis sativus protoplasts transfected with in vitro transcripts. without rna2, rna1 replicated efficientl ...201626982585
glucosinolate desulfation by the phloem-feeding insect bemisia tabaci.glucosinolates are plant secondary defense metabolites confined nearly exclusively to the order brassicales. upon tissue rupture, glucosinolates are hydrolyzed to various bioactive breakdown products by the endogenous plant enzyme myrosinase. as the feeding of chewing insect herbivores is associated with plant tissue damage, these insects have developed several independent strategies for coping with the glucosinolate-myrosinase defense system. on the other hand, our knowledge of how phloem-feedi ...201626961756
role of the insect supervectors bemisia tabaci and frankliniella occidentalis in the emergence and global spread of plant viruses.emergence of insect-transmitted plant viruses over the past 10-20 years has been disproportionately driven by two so-called supervectors: the whitefly, bemisia tabaci, and the western flower thrips, frankliniella occidentalis. high rates of reproduction and dispersal, extreme polyphagy, and development of insecticide resistance, together with human activities, have made these insects global pests. these supervectors transmit a diversity of plant viruses by different mechanisms and mediate virus ...201526958907
rapid assessment of insect fauna based on local knowledge: comparing ecological and ethnobiological methods.the rapid assessment of biodiversity making use of surveys of local knowledge has been successful for different biological taxa. however, there are no reports on the testing of such tools for sampling insect fauna. the present study aimed to evaluate the efficiency of different ethnobiological techniques for rapid sampling of insect fauna.201626932264
survey of indigenous entomopathogenic fungi and evaluation of their pathogenicity against the carmine spider mite, tetranychus cinnabarinus (boisd.), and the whitefly, bemisia tabaci (genn.) biotype b.the carmine spider mite, tetranychus cinnabarinus, and the silverleaf whitefly, bemisia tabaci, are serious pests of both field- and greenhouse-grown crops in south-western turkey. control of these pests has been heavily dependent upon chemical pesticides. the objectives of this study were to investigate the occurrence of indigenous entomopathogenic fungi (epf) in field populations of t. cinnabarinus and b. tabaci, and to evaluate their pathogenicity against these pests. for this purpose, a surv ...201626929052
transcriptome analysis reveals a comprehensive insect resistance response mechanism in cotton to infestation by the phloem feeding insect bemisia tabaci (whitefly).the whitefly (bemisia tabaci) causes tremendous damage to cotton production worldwide. however, very limited information is available about how plants perceive and defend themselves from this destructive pest. in this study, the transcriptomic differences between two cotton cultivars that exhibit either strong resistance (hr) or sensitivity (zs) to whitefly were compared at different time points (0, 12, 24 and 48 h after infection) using rna-seq. approximately one billion paired-end reads were o ...201626923339
reproduction of the whitefly bemisia tabaci (hemiptera: aleyrodidae) b biotype in maize fields (zea mays l.) in brazil.bemisia tabaci (genn.) (hemiptera: aleyrodidae) was observed to have completed its reproductive cycle from the egg to the adult on maize (zea mays l.). field and screenhouse studies were carried out to investigate the durability of this putative and unprecedented adaptation to a grass host.201626910287
a primary screening and applying of plant volatiles as repellents to control whitefly bemisia tabaci (gennadius) on tomato.with the goal of finding a new way to reduce population densities of bemisia tabaci biotype q in greenhouses, seven repellent volatile chemicals and their combinations were screened. the mixture of dlco (d-limonene, citral and olive oil (63:7:30)) had a better cost performance(sc50 = 22.59 mg/ml)to repel whiteflies from settling than the other mixtures or single chemicals. in the greenhouse, in both the choice test and the no-choice tests, the number of adult whiteflies that settled on 1% dlco-t ...201626907368
effects of spirotetramat treatments on fecundity and carboxylesterase expression of aphis gossypii glover.spirotetramat is a novel tetramic acid-based insecticide, belonging to keto-enol pesticide family, with a novel mode of action; it interferes with lipid biosynthesis. its insecticide activity against various agricultural pest insects have been demonstrated (e.g. on myzus persicae, bemisia tabaci and tetranychus urticae). however, information available is currently limited on the efficacy of spirotetramat on the cotton aphid, aphis gossypii, a key cotton pest worldwide. we assessed the spirotetra ...201626898726
evolutionary conservation of candidate osmoregulation genes in plant phloem sap-feeding insects.the high osmotic pressure generated by sugars in plant phloem sap is reduced in phloem-feeding aphids by sugar transformations and facilitated water flux in the gut. the genes mediating these osmoregulatory functions have been identified and validated empirically in the pea aphid acyrthosiphon pisum: sucrase 1 (suc1), a sucrase in glycoside hydrolase family 13 (gh13), and aquaporin 1 (aqp1), a member of the drosophila integral protein (drip) family of aquaporins. here, we describe molecular anal ...201626896054
repellent and attractive effects of α-, β-, and dihydro-β- ionone to generalist and specialist herbivores.in plants, the oxidative cleavage of carotenoid substrates produces volatile apocarotenoids, including α-ionone, β-ionone, and dihydro-β-ionone, compounds that are important in herbivore-plant communication. for example, β-ionone is part of an induced defense in canola, brassica napus, and is released following wounding by herbivores. the objectives of the research were to evaluate whether these volatile compounds would: 1) be released in higher quantities from plants through the over-expression ...201626852133
microrna profiling of the whitefly bemisia tabaci middle east-aisa minor i following the acquisition of tomato yellow leaf curl china virus.the begomoviruses are the largest and most economically important group of plant viruses exclusively vectored by whitefly (bemisia tabaci) in a circulative, persistent manner. during this process, begomoviruses and whitefly vectors have developed close relationships and complex interactions. however, the molecular mechanisms underlying these interactions remain largely unknown, and the microrna profiles for viruliferous and nonviruliferous whiteflies have not been studied.201626837429
oogenesis in the bemisia tabaci meam1 species complex.the whitefly bemisia tabaci meam1 species complex has invaded several parts of the world in the past 30 years and replaced native whitefly populations in the invaded regions, including certain areas of china. one of the possible reasons for the invasion is that meam1 whiteflies are more fecund than native species. however, the factors that affect the reproduction of the b. tabaci cryptic species are not clearly known. the regulation of oogenesis is thought to be one of the essential processes fo ...201626826802
computational insights into the different resistance mechanism of imidacloprid versus dinotefuran in bemisia tabaci.insecticide resistance is a critical problem for pest control and management. for bemisia tabaci, striking high metabolic resistance (generally conferred by cyp6cm1) was observed for imidacloprid (imi) and most other neonicotinoid members. however, dinotefuran (din) displayed very low resistance factors, which indicated distinct metabolic properties. here, molecular modeling methods were applied to explore the different resistance features of imi versus din within the q type of cyp6cm1. it was f ...201626817991
molecular cloning of the sex-related gene psi in bemisia tabaci and its alternative splicing properties.the p-element somatic inhibitor (psi) is gene known to regulate the transcription of doublesex (dsx) when transformer (tra) is absent in bombyx mori. in this study, we identified and characterized a psi homolog in bemisia tabaci (btpsi). btpsi cdna had a total length of 5700 bp and contained a predicted open reading frame (orf) of 2208 nucleotides encoding for 735 amino acids. multiple sequence alignments of the common regions of psi proteins from b. tabaci and five other insect species revealed ...201626773355
rna1-independent replication and gfp expression from tomato marchitez virus isolate m cloned cdna.tomato marchitez virus (tomarv; synonymous with tomato apex necrosis virus) is a positive-strand rna virus in the genus torradovirus within the family secoviridae. tomarv is an emergent whitefly-transmitted virus that causes important diseases in tomato (solanum lycopersicum) in mexico. here, the genome sequence of the tomarv isolate m (tomarv-m) was determined. we engineered full-length cdna clones of the tomarv-m genomic rna (rna1 and rna2), separately, into a binary vector. coinfiltration of ...201626756828
tomato yellow leaf curl virus (tylcv-il): a seed-transmissible geminivirus in tomatoes.tomato yellow leaf curl virus (tylcv) is one of the most well-known tomato-infecting begomoviruses and transmitted by bemisia tabaci. seed transmission has previously been reported for some rna viruses, but tylcv has not previously been described as a seed-borne virus. in 2013 and 2014, without whitefly-mediated transmission, tylcv was detected in young tomato plants germinated from fallen fruits produced from tylcv-infected tomato plants in the previous cultivation season. in addition, tylcv-is ...201626743765
lethal and inhibitory activities of plant-derived essential oils against bemisia tabaci gennadius (hemiptera: aleyrodidae) biotype b in tomato.the silverleaf whitefly bemisia tabaci (genn.) biotype b (hemiptera: aleyrodidae) is one of the most severe tomato pests in the world. the damage caused by this insect may compromise up to 100% of crop production, and management of this pest has relied on spraying of synthetic insecticides. however, due to the environmental issues associated with this practice, alternative methods such as the use of botanical pesticides are now used as a strategy of integrated pest management (ipm). we evaluated ...201626712319
use of lablab (lablab purpureus (l.) sweet) for bio-control by native arthropods and its effect on yield of pumpkins.silverleaf whitefly (slw, bemisia tabaci meam1) and aphids are sap-sucking insects, which pose a serious threat to australian cucurbit crops and the horticulture industry. traditional chemical control for these insect pests is becoming less effective, and there is a need to search for alternative or supplementary methods. this study aimed to manipulate the habitat of pumpkin crops in a tropical setting (queensland, australia), by growing pumpkins (var. japanese pumpkin) alone and between lablab ...201626693799
only a minority of broad-range detoxification genes respond to a variety of phytotoxins in generalist bemisia tabaci species.generalist insect can utilize two different modes for regulating their detoxification genes, the constitutive mode and the induced mode. here, we used the bemisia tabaci sibling species meam1 and med, as a model system for studying constitutive and induced detoxification resistance and their associated tradeoffs. b. tabaci adults were allowed to feed through membranes for 24 h on diet containing only sucrose or sucrose with various phytotoxins. quantitative real-time pcr analyses of 18 detoxific ...201526655836
tomato yellow leaf curl virus confronts host degradation by sheltering in small/midsized protein aggregates.tomato yellow leaf curl virus (tylcv) is a begomovirus transmitted by the whitefly bemisia tabaci to tomato and other crops. tylcv proteins are endangered by the host defenses. we have analyzed the capacity of the tomato plant and of the whitefly insect vector to degrade the six proteins encoded by the tylcv genome. tomato and whitefly demonstrated the highest proteolytic activity in the fractions containing soluble proteins, less-in large protein aggregates; a significant decrease of tylcv prot ...201626654789
proteome analysis of bemisia tabaci suggests specific targets for rnai mediated control.rna interference offers effective control of several economically important insect pests. bemisia tabaci is an important field crop pest, which causes significant yield loss worldwide. in our earlier study, we have demonstrated successful control of b. tabaci through transgenic plant mediated rnai. however, selection of target genes without off-target effect(s) has been major concern so far and therefore, a critical exploration for b. tabaci specific targets is frantically required. in this stud ...201626646750
aboveground whitefly infestation modulates transcriptional levels of anthocyanin biosynthesis and jasmonic acid signaling-related genes and augments the cope with drought stress of maize.up to now, the potential underlying molecular mechanisms by which maize (zea mays l.) plants elicit defense responses by infestation with a phloem feeding insect whitefly [bemisia tabaci (genn.)] have been barely elucidated against (a)biotic stresses. to fill this gap of current knowledge maize plants were infested with whitefly and these plants were subsequently assessed the levels of water loss. to understand the mode of action, plant hormone contents and the stress-related mrna expression wer ...201526630288
rapid accumulation and low degradation: key parameters of tomato yellow leaf curl virus persistence in its insect vector bemisia tabaci.of worldwide economic importance, tomato yellow leaf curl virus (tylcv, begomovirus) is responsible for one of the most devastating plant diseases in warm and temperate regions. the dna begomoviruses (geminiviridae) are transmitted by the whitefly species complex bemisia tabaci. although geminiviruses have long been described as circulative non-propagative viruses, observations such as long persistence of tylcv in b. tabaci raised the question of their possible replication in the vector. we moni ...201526625871
quantitative resistance against bemisia tabaci in solanum pennellii: genetics and metabolomics.the whitefly bemisia tabaci is a serious threat in tomato cultivation worldwide as all varieties grown today are highly susceptible to this devastating herbivorous insect. many accessions of the tomato wild relative solanum pennellii show a high resistance towards b. tabaci. a mapping approach was used to elucidate the genetic background of whitefly-resistance related traits and associated biochemical traits in this species. minor quantitative trait loci (qtls) for whitefly adult survival (as) a ...201626576823
transcription analysis of neonicotinoid resistance in mediterranean (med) populations of b. tabaci reveal novel cytochrome p450s, but no nachr mutations associated with the phenotype.bemisia tabaci is one of the most damaging agricultural pests world-wide. although its control is based on insecticides, b. tabaci has developed resistance against almost all classes of insecticides, including neonicotinoids.201526573457
control of the tomato leafminer, tuta absoluta (lepidoptera: gelechiidae), in open-field tomatoes by indigenous natural enemies occurring in israel.the tomato leafminer, tuta absoluta (meyrick), had established in israel by 2010, attacking both open-field tomatoes and greenhouse crops.we searched for its natural enemies in open-field tomatoes, and tried to determine their potential for controlling this pest. we surveyed the local natural enemies in open tomato fields and measured their impact on pest populations in an unsprayed field. we assessed the suppressive ability of the dominant hemipteran predator, nesidiocoris tenuis reuter, agains ...201626567332
temporal effects of a begomovirus infection and host plant resistance on the preference and development of an insect vector, bemisia tabaci, and implications for epidemics.persistent plant viruses, by altering phenotypic and physiological traits of their hosts, could modulate the host preference and fitness of hemipteran vectors. a majority of such modulations increase vector preference for virus-infected plants and improve vector fitness, ultimately favouring virus spread. nevertheless, it remains unclear how these virus-induced modulations on vectors vary temporally, and whether host resistance to the pathogen influences such effects. this study addressed the tw ...201526529402
vector-enabled metagenomic (vem) surveys using whiteflies (aleyrodidae) reveal novel begomovirus species in the new and old worlds.whitefly-transmitted viruses belonging to the genus begomovirus (family geminiviridae) represent a substantial threat to agricultural food production. the rapid evolutionary potential of these single-stranded dna viruses combined with the polyphagous feeding behavior of their whitefly vector (bemisia tabaci) can lead to the emergence of damaging viral strains. therefore, it is crucial to characterize begomoviruses circulating in different regions and crops globally. this study utilized vector-en ...201526516898
differential temporal changes of primary and secondary bacterial symbionts and whitefly host fitness following antibiotic treatments.where multiple symbionts coexist in the same host, the selective elimination of a specific symbiont may enable the roles of a given symbiont to be investigated. we treated the mediterranean species of the whitefly bemisia tabaci complex by oral delivery of the antibiotic rifampicin, and then examined the temporal changes of its primary symbiont "candidatus portiera aleyrodidarum" and secondary symbiont "ca. hamiltonella defensa" as well as host fitness for three generations. in adults treated wi ...201526510682
a bemisia tabaci midgut protein interacts with begomoviruses and plays a role in virus transmission.begomoviruses are a major group of plant viruses, transmitted exclusively by bemisia tabaci (gennadius) in a persistent circulative non-propagative manner. the information regarding molecular and cellular basis underlying begomovirus - whitefly interaction is very scarce. evidences have suggested that the insect gut possesses some crucial protein receptors that allow specific entry of virus into the insect haemolymph. we have performed yeast two hybrid gut cdna expression library screening again ...201626488331
comparison of transmission of papaya leaf curl china virus among four cryptic species of the whitefly bemisia tabaci complex.begomoviruses are transmitted by cryptic species of the whitefly bemisia tabaci complex, often in a species-specific manner. papaya leaf curl china virus (palcucnv) has been recorded to infect several crops including papaya, tomato and tobacco in china. to help assess the risks of spread of this virus, we compared the acquisition, retention and transmission of palcucnv among four species of whiteflies, middle east-asia minor 1 (meam1), mediterranean (med), asia 1 and asia ii 7. all four species ...201526486606
sequence variation of bemisia tabaci chemosensory protein 2 in cryptic species b and q: new dna markers for whitefly recognition.bemisia tabaci gennadius biotypes b and q are two of the most important worldwide agricultural insect pests. genomic sequences of type-2 b. tabaci chemosensory protein (btabcsp2) were cloned and sequenced in b and q biotypes, revealing key biotype-specific variations in the intron sequence. a q260 sequence was found specifically in q-btabcsp2 and cucumis melo ln692399, suggesting ancestral horizontal transfer of gene between the insect and the plant through bacteria. a cleaved amplified polymorp ...201626481237
behavioral response of bemisia tabaci (hemiptera: aleyrodidae) to 20 plant extracts.in the mediterranean region, the use of small-mesh netting to protect horticultural crops is an effective sustainable tool against pests. but in tropical regions, because of high humidity under the net favoring fungal development, netting with a larger mesh size has to be used, protecting crops against lepidopteran pests but not against small pests such as hemipterans, thrips, and phytophagous mites. a combination of netting with a repellent or irritant product is one possible solution, but the ...201526470332
effectiveness of cyantraniliprole for managing bemisia tabaci (hemiptera: aleyrodidae) and interfering with transmission of tomato yellow leaf curl virus on tomato.cyantraniliprole is the second xylem-systemic active ingredient in the new anthranilic diamide class. greenhouse (2006), growth chamber (2007), and field studies (2009-2010) were conducted to determine the efficacy of cyantraniliprole for managing bemisia tabaci (gennadius) biotype b and in interfering with transmission of tomato yellow leaf curl virus (tylcv) by this whitefly. cyantraniliprole applied as soil treatments (200 sc) or foliar sprays (100 od) provided excellent adult whitefly contro ...201526470209
three members of the bemisia tabaci (hemiptera: aleyrodidae) cryptic species complex occur sympatrically in argentine horticultural crops.the whitefly, bemisia tabaci (gennadius), is a cryptic species complex that attacks >600 different species of plants and transmits several plant viruses causing severe economic losses. until 2010, the b. tabaci complex comprised 24 distinct putative species. recently, at least 15 new species have been reported. the objective of this study was to identify b. tabaci species present in bean, melon, and tomato crops in argentina by applying phylogenetic analyses and pairwise comparison of genetic di ...201526470151
effects of host sex, plant species, and putative host species on the prevalence of wolbachia in natural populations of bemisia tabaci (hemiptera: aleyrodidae): a modified nested pcr study.bemisia tabaci (gennadius) (hemiptera: aleyrodidae) is a globally distributed pest. one of the key endosymbionts in b. tabaci is wolbachia, an α-proteobacterium implicated in many important biological processes. previous studies indicated that the infection frequency of wolbachia in middle east-asia minor 1 (meam1) and mediterranean (med) varied greatly among populations in different areas. however, little is known about the factors that influence the prevalence of wolbachia in b. tabaci. in thi ...201526470122
potential of five brazilian populations of phytoseiidae (acari) for the biological control of bemisia tabaci (insecta: hemiptera).biotype b of the whitefly, bemisia tabaci (gennadius) (hemiptera: aleyrodidae), causes considerable losses to growers worldwide. phytoseiid mites have been successfully used for the control of this pest in several countries. the brazilian phytoseiid fauna is very diverse and potentially useful for this purpose. the aim of this study was to evaluate five brazilian populations of phytoseiids as candidates for the control of the whitefly, a serious pest of different crops worldwide. evaluated speci ...201526470100
manipulation of host quality and defense by a plant virus improves performance of whitefly vectors.pathogen-mediated interactions between insect vectors and their host plants can affect herbivore fitness and the epidemiology of plant diseases. while the role of plant quality and defense in mediating these tripartite interactions has been recognized, there are many ecologically and economically important cases where the nature of the interaction has yet to be characterized. the bemisia tabaci (gennadius) cryptic species mediterranean (med) is an important vector of tomato yellow leaf curl viru ...201526470098
update on the status of bemisia tabaci in the uk and the use of entomopathogenic fungi within eradication programmes.the sweetpotato whitefly bemisia tabaci (gennadius) (hemiptera: aleyrodidae) continues to be a serious threat to crops worldwide. the uk holds protected zone status against this pest and, as a result, b. tabaci entering on plant material is subjected to a policy of eradication. both b and q bemisia biotypes are now regularly intercepted entering the uk. with increasing reports of neonicotinoid resistance in both these biotypes, it is becoming more problematic to control/eradicate. therefore, alt ...201326464385
estimation of the whitefly bemisia tabaci genome size based on k-mer and flow cytometric analyses.whiteflies of the bemisia tabaci (hemiptera: aleyrodidae) cryptic species complex are among the most important agricultural insect pests in the world. these phloem-feeding insects can colonize over 1000 species of plants worldwide and inflict severe economic losses to crops, mainly through the transmission of pathogenic viruses. surprisingly, there is very little genomic information about whiteflies. as a starting point to genome sequencing, we report a new estimation of the genome size of the b ...201526463411
electrostatic insect sweeper for eliminating whiteflies colonizing host plants: a complementary pest control device in an electric field screen-guarded greenhouse.our greenhouse tomatoes have suffered from attacks by viruliferous whiteflies bemisia tabaci (gennadius) (hemiptera: aleyrodidae) over the last 10 years. the fundamental countermeasure was the application of an electric field screen to the greenhouse windows to prevent their entry. however, while the protection was effective, it was incomplete, because of the lack of a guard at the greenhouse entrance area; in fact, the pests entered from the entrance door when workers entered and exited. to add ...201526463195
the importance of maintaining protected zone status against bemisia tabaci.the sweetpotato whitefly, bemisia tabaci (gennadius) (hemiptera: aleyrodidae) is a major pest of economically important crops worldwide. both the united kingdom (uk) and finland hold protected zone status against this invasive pest. as a result b. tabaci entering these countries on plants and plant produce is subjected to a policy of eradication. the impact of b. tabaci entering, and becoming established, is that it is an effective vector of many plant viruses that are not currently found in the ...201526463194
tri-tek (petroleum horticultural oil) and beauveria bassiana: use in eradication strategies for bemisia tabaci mediterranean species in uk glasshouses.the sweetpotato whitefly bemisia tabaci (gennadius) (hemiptera: aleyrodidae) is a pest of global importance on both outdoor and glasshouse crops. to date, b. tabaci has not become established in the uk. the uk holds protected zone status against this pest and, as a result, b. tabaci entering on plant material is subjected to a policy of eradication. mediterranean species is now the most prevalent bemisia species entering the uk. increasing neonicotinoid resistance is becoming increasingly widesp ...201526463071
densities of eggs and nymphs and percent parasitism of bemisia tabaci (hemiptera: aleyrodidae) on common weeds in west central florida.the density of eggs and nymphs of bemisia tabaci (gennadius) biotype b and the percent parasitism of the nymphs were measured from specimens collected on nine species of weeds, commonly found in west central florida during the spring and summer of 2012 and 2013. the weeds were direct seeded in 2012 and grown as transplants in 2013 for randomized complete block design experiments. the leaf area of each whole-plant sample was measured and the b. tabaci density parameters were converted to numbers ...201426462945
the feeding rate of predatory mites on life stages of bemisia tabaci mediterranean species.the sweetpotato whitefly bemisia tabaci (gennadius) (hemiptera: aleyrodidae) continues to be a serious threat to crops worldwide. the uk holds protected zone status against this pest and, as a result, b. tabaci entering on plant material is subjected to a policy of eradication. there has recently been a shift from middle east-asia minor 1 to the more chemical resistant mediterranean species entering the uk. predatory mites (amblyseius swirskii, transeius montdorensis and typhlodromalus limonicus ...201426462828
are mummies and adults of eretmocerus mundus (hymenoptera: aphelinidae) compatible with modern insecticides?the parasitic wasp eretmocerus mundus (mercet) is an important natural enemy of the widespread key pest bemisia tabaci (gennadius). the toxicity of 11 modern insecticides applied at their maximum field recommended rate in spain was tested in two life stages of e. mundus: adults and mummies. laboratory and persistence tests were conducted and effects assessed not only in terms of mortality but on reproductive performance as well. some insecticides caused the same effect to both life stages: flube ...201526453715
tomato yellow leaf curl virus differentially influences plant defence responses to a vector and a non-vector herbivore.plants frequently engage in simultaneous interactions with diverse classes of biotic antagonists. differential induction of plant defence pathways by these antagonists, and interactions between pathways, can have important ecological implications; however, these effects are currently not well understood. we explored how tomato yellow leaf curl virus (tylcv) influenced the performance of its vector (bemisia tabaci) and a non-vector herbivore (tetranychus urticae) occurring separately or together ...201626436779
prey preference and life table of amblyseius orientalis on bemisia tabaci and tetranychus cinnabarinus.amblyseius orientalis (ehara) (acari: phytoseiidae) is a native predatory mite species in china. it used to be considered as a specialist predator of spider mites. however, recent studies show it also preys on other small arthropod pests, such as bemisia tabaci (gennadius) (hemiptera: aleyrodidae). experiments were conducted to investigate (1) prey preference of a. orientalis between tetranychus cinnabarinus (boisd.) (acari: tetranychidae) and b. tabaci, and (2) development, consumption and life ...201526436422
transgenic cotton-fed bemisia tabaci (gennadius) (hemiptera: aleyrodidae) affects the parasitoid encarsia desantisi viggiani (hymenoptera: aphelinidae) development.cotton cultivars expressing cry proteins are widely used to control lepidopteran pests. the effects of transgenic plants containing insecticidal cry proteins on non-target species must be comprehended for a better and rational use of this technology for pest management. we investigated the influence of the bt cotton cultivars nuopal and fm 975 on biological parameters of bemisia tabaci (gennadius) (hemiptera: aleyrodidae), a non-target pest of bt cotton cultivars and on its parasitoid encarsia d ...201626431703
temporal changes of symbiont density and host fitness after rifampicin treatment in a whitefly of the bemisia tabaci species complex.microbial symbionts are essential or important partners to phloem-feeding insects. antibiotics have been used to selectively eliminate symbionts from their host insects and establish host lines with or without certain symbionts for investigating functions of the symbionts. in this study, using the antibiotic rifampicin we attempted to selectively eliminate certain symbionts from a population of the middle east-asia minor 1 whitefly of the bemisia tabaci species complex, which harbors the primary ...201626412633
preference of bemisia tabaci biotype b on zucchini squash and buckwheat and the effect of delphastus catalinae on whitefly populations.zucchini squash, cucurbita pepo l., is an important vegetable crop in florida. physiological disorders and insect-transmitted diseases are major problems for squash growers in semi-tropical regions around the world. bemisia tabaci (gennadius) biotype b is a significant whitefly pest and is largely responsible for transmitting viruses and causing physiological disorders in squash. several studies have shown that whitefly populations are reduced when crops are interplanted with non-host cover crop ...201626388111
potential of two populations of amblyseius swirskii (acari: phytoseiidae) for the control of bemisia tabaci biotype b (hemiptera: aleyrodidae) in brazil.predatory mites of the family phytoseiidae are commercialized in european and north american countries for the control of whiteflies (insecta: aleyrodidae). amblyseius swirskii athias-henriot (acari: phytoseiidae) is one of the predators used for that purpose. this predator is not found in brazil and in many other countries, but its introduction could promote biological control of bemisia tabaci (gennadius) biotype b (silverleaf whitefly) in those countries. the aim of this study was to compare ...201526387112
feeding of whitefly on tobacco decreases aphid performance via increased salicylate signaling.the feeding of bemisia tabaci nymphs trigger the sa pathway in some plant species. a previous study showed that b. tabaci nymphs induced defense against aphids (myzus persicae) in tobacco. however, the mechanism underlying this defense response is not well understood.201526381273
metabolic coevolution in the bacterial symbiosis of whiteflies and related plant sap-feeding insects.genomic decay is a common feature of intracellular bacteria that have entered into symbiosis with plant sap-feeding insects. this study of the whitefly bemisia tabaci and two bacteria (portiera aleyrodidarum and hamiltonella defensa) cohoused in each host cell investigated whether the decay of portiera metabolism genes is complemented by host and hamiltonella genes, and compared the metabolic traits of the whitefly symbiosis with other sap-feeding insects (aphids, psyllids, and mealybugs). paral ...201526377567
conditional fitness benefits of the rickettsia bacterial symbiont in an insect pest.inherited bacterial symbionts are common in arthropods and can have strong effects on the biology of their hosts. these effects are often mediated by host ecology. the rickettsia symbiont can provide strong fitness benefits to its insect host, bemisia tabaci, under laboratory and field conditions. however, the frequency of the symbiont is heterogeneous among field collection sites across the usa, suggesting that the benefits of the symbiont are contingent on additional factors. in two whitefly g ...201626376661
[the effects of transgenic cry1ac+cry2ab cotton on cotton bollworm control and functional response of predators on whitefly].in this study, we detected and clarified the roles of transgenic cry1ac+cry2ab cotton "639020" in controlling cotton bollworm (helicoverpa armigera) during critical periods of bud stage (second generation of bollworm), flowering stage (third generation of bollworm) and bolling stage (fourth generation of bollworm) as well as the influences of 639020 cotton on functional response of the main predators (chrysopa sinica larvae, propylaea japonica, orius and erigonidium graminicola ) on whitefly usi ...201526351054
aphid-induced defences in chilli affect preferences of the whitefly, bemisia tabaci (hemiptera: aleyrodidae).the sweetpotato whitefly (wf), bemisia tabaci, is a major pest that damages a wide range of vegetable crops in malaysia. wf infestation is influenced by a variety of factors, including previous infestation of the host plant by other insect pests. this study investigated the effects of previous infestation of host chilli plants by the green peach aphid (myzus persicae) on the olfactory behavioural response of b. tabaci, using free-choice bioassay with a y-tube olfactometer. we analysed volatile o ...201526334135
distribution of bemisia tabaci genetic groups in india.the whitefly bemisia tabaci (gennadius) (hemiptera: aleyrodidae) is a phloem-feeding, economically important pest of crops worldwide. in addition to direct damage, it also vectors a number of plant viruses belonging to the family geminiviridae. its populations differ biologically with respect to insecticide resistance, virus transmission and host range. therefore, understanding genetic variation among populations is important for management. we sequenced 850 bp of the mitochondrial coi (mtcoi) g ...201526314072
evaluating weeds as hosts of tomato yellow leaf curl virus.bemisia tabaci (gennadius) biotype b transmits tomato yellow leaf curl virus (tylcv), which affects tomato production globally. prompt destruction of virus reservoirs is a key component of virus management. identification of weed hosts of tylcv will be useful for reducing such reservoirs. the status of weeds as alternate hosts of tylcv in florida remains unclear. in greenhouse studies, b. tabaci adults from a colony reared on tylcv-infected tomato were established in cages containing one of four ...201526314055
generating within-plant spatial distributions of an insect herbivore based on aggregation patterns and per-node infestation probabilities.most predator-prey models extrapolate functional responses from small-scale experiments assuming spatially uniform within-plant predator-prey interactions. however, some predators focus their search in certain plant regions, and herbivores tend to select leaves to balance their nutrient uptake and exposure to plant defenses. individual-based models that account for heterogeneous within-plant predator-prey interactions can be used to scale-up functional responses, but they would require the gener ...201526313173
reduction of viral load in whitefly (bemisia tabaci gen.) feeding on rnai-mediated bean golden mosaic virus resistant transgenic bean plants.the rnai concept was explored to silence the rep gene from the bean golden mosaic virus (bgmv) and a genetically modified (gm) bean immune to the virus was previously generated. we investigated if bgmv-viruliferous whiteflies would reduce viral amount after feeding on gm plants. bgmv dna amount was significantly reduced in whiteflies feeding in gm-plants (compared with insects feeding on non-gm plants) for a period of 4 and 8 days in 52% and 84% respectively.201526297125
infection of bacterial endosymbionts in insects: a comparative study of two techniques viz pcr and fish for detection and localization of symbionts in whitefly, bemisia tabaci.bacterial endosymbionts have been associated with arthropods and large number of the insect species show interaction with such bacteria. different approaches have been used to understand such symbiont- host interactions. the whitefly, bemisia tabaci, a highly invasive agricultural pest, harbors as many as seven different bacterial endosymbionts. these bacterial endosymbionts are known to provide various nutritional, physiological, environmental and evolutionary benefits to its insect host. in th ...201526287997
differential expression patterns among heat-shock protein genes and thermal responses in the whitefly bemisia tabaci (meam 1).there is convincing evidence that heat-shock proteins (hsp) are upregulated by stress conditions in insects; however, the relative contribution of each hsp gene to the heat-shock response remains unclear. here we considered the whitefly bemisia tabaci (meam 1), a phloem feeder and invasive species whose molecular stress response is an important mechanism for overcoming heat stress. we assessed the expression of the hsp23, 40, 70 and 90 genes at the mrna level when submitted to heat shocks of 40 ...201526267515
pathogenicity of isaria fumosorosea to bemisia tabaci, with some observations on the fungal infection process and host immune response.isaria fumosorosea is an important pathogen of whiteflies, and is used as a mycoinsecticide against this pest in many regions of the world. we quantified the pathogenicity of the chinese isolate if-1106 against different life stages of sweetpotato whitefly, bemisia tabaci, on cucumber plants, and describe the infection process and aspects of the host immunological response in the laboratory. the second instar was the most susceptible life stage to infection, with mortality rates at 10(7)conidia/ ...201526264671
expression of dsrna in recombinant isaria fumosorosea strain targets the tlr7 gene in bemisia tabaci.rna interference (rnai) technology shows a great potential in controlling agricultural pests, despite the difficulty of introducing exogenous dsrna/sirna into target pests. isaria fumosorosea is a common fungal pathogen of the b-biotype bemisia tabaci (whitefly), which is a widespread pest. entomopathogenic fungi directly penetrate the cuticle and invade insect hemocoel. application of i. fumosorosea expressing dsrna of whitefly immunity-related gene may aid in developing rnai technology to effe ...201526198409
persistent, circulative transmission of begomoviruses by whitefly vectors.begomoviruses comprise an emerging and economically important group of plant viruses exclusively transmitted by the sweetpotato whitefly bemisia tabaci in many regions of the world. the past twenty years have witnessed significant progress in studying the molecular interactions between members of this virus group and b. tabaci. mechanisms and proteins encoded by the insect vector and its bacterial symbionts, which have been shown to be important for virus transmission, have been identified and t ...201526196230
replication of tomato yellow leaf curl virus in its whitefly vector, bemisia tabaci.tomato yellow leaf curl virus (tylcv) is a begomovirus transmitted exclusively by the whitefly bemisia tabaci in a persistent, circulative manner. replication of tylcv in its vector remains controversial, and thus far, the virus has been considered to be nonpropagative. following 8 h of acquisition on tylcv-infected tomato plants or purified virions and then transfer to non-tylcv-host cotton plants, the amounts of virus inside whitefly adults significantly increased (>2-fold) during the first fe ...201526178995
how active ingredient localisation in plant tissues determines the targeted pest spectrum of different chemistries.the efficacies of four commercial insecticides and of two research compounds were tested against aphids (aphis craccivora and myzus persicae), whiteflies (bemisia tabaci) and red-spotted spider mites (tetranychus urticae) in intrinsic (oral administration), curative (direct contact spray) and translaminar (arthropods infested on untreated leaf underside) assays. with a new translaminar model, the transport across the leaf cuticle and tissues and the electrochemical distribution of test compounds ...201626112169
flupyradifurone (sivanto™) and its novel butenolide pharmacophore: structural considerations.flupyradifurone (4-[(2,2-difluoroethyl)amino]-2(5h)-furanone), a member of the new class of butenolide insecticides, contains a novel bioactive scaffold as pharmacophore. it is very versatile in terms of application methods to a variety of crops, exhibits excellent and fast action against a broad spectrum of sucking pest insects including selected neonicotinoid resistant pest populations such as whiteflies and aphids expressing metabolic resistance mechanisms. as a partial agonist flupyradifuron ...201526047109
development of a lateral flow test to detect metabolic resistance in bemisia tabaci mediated by cyp6cm1, a cytochrome p450 with broad spectrum catalytic efficiency.cotton whitefly, bemisia tabaci (genn.) (homoptera: aleyrodidae) is a major sucking pest in many agricultural and horticultural cropping systems globally. the frequent use of insecticides of different mode of action classes resulted in populations resisting treatments used to keep numbers under economic damage thresholds. recently it was shown that resistance to neonicotinoids such as imidacloprid is linked to the over-expression of cyp6cm1, a cytochrome p450 monooxygenase detoxifying imidaclopr ...201526047106
flow cytometry and k-mer analysis estimates of the genome sizes of bemisia tabaci b and q (hemiptera: aleyrodidae).the genome sizes of the b- and q-types of the whitefly bemisia tabaci (gennnadius) were estimated using flow cytometry (drosophila melanogaster as the dna reference standard and propidium iodide (pi) as the fluorochrome) and k-mer analysis. for flow cytometry, the mean nuclear dna content was 0.686 pg for b-type males, 1.392 pg for b-type females, 0.680 pg for q-type males, and 1.306 pg for q-type females. based on the relationship between dna content and genome size (1 pg dna = 980 mbp), the ha ...201526042041
transcriptional responses of invasive and indigenous whiteflies to different host plants reveal their disparate capacity of adaptation.the whitefly bemisia tabaci contains more than 35 cryptic species. the higher adaptability of middle east-asia minor 1 (meam1) cryptic species has been recognized as one important factor for its invasion and displacement of other indigenous species worldwide. here we compared the performance of the invasive meam1 and the indigenous asia ii 3 whitefly species following host plant transfer from a suitable host (cotton) to an unsuitable host (tobacco) and analyzed their transcriptional responses. a ...201526041313
salicylic acid is required for mi-1-mediated resistance of tomato to whitefly bemisia tabaci, but not for basal defense to this insect pest.plant defense to pests or pathogens involves global changes in gene expression mediated by multiple signaling pathways. a role for the salicylic acid (sa) signaling pathway in mi-1-mediated resistance of tomato (solanum lycopersicum) to aphids was previously identified and its implication in the resistance to root-knot nematodes is controversial, but the importance of sa in basal and mi-1-mediated resistance of tomato to whitefly bemisia tabaci had not been determined. sa levels were measured be ...201526032615
transmission of tomato yellow leaf curl virus by bemisia tabaci as affected by whitefly sex and biotype.bemisia tabaci is a serious pest of vegetables and other crops worldwide. the most damaging and predominant b. tabaci biotypes are b and q, and both are vectors of tomato yellow leaf curl virus (tylcv). previous research has shown that q outperforms b in many respects but comparative research is lacking on the ability of b and q to transmit viruses. in the present study, we tested the hypothesis that b and q differ in their ability to transmit tylcv and that this difference helps explain tylcv o ...201526021483
recessive resistance derived from tomato cv. tyking-limits drastically the spread of tomato yellow leaf curl virus.the tomato yellow leaf curl disease (tylcd) causes severe damage to tomato (solanum lycopersicum l.) crops throughout tropical and subtropical regions of the world. tylcd is associated with a complex of single-stranded circular dna plant viruses of the genus begomovirus (family geminiviridae) transmitted by the whitefy bemisia tabaci gennadius (hemiptera: aleyrodidae). the tomato inbred line tx 468-rg is a source of monogenic recessive resistance to begomoviruses derived from the hybrid cv. tyki ...201526008699
whitefly genome expression reveals host-symbiont interaction in amino acid biosynthesis.whitefly (bemisia tabaci) complex is a serious insect pest of several crop plants worldwide. it comprises several morphologically indistinguishable species, however very little is known about their genetic divergence and biosynthetic pathways. in the present study, we performed transcriptome sequencing of asia 1 species of b. tabaci complex and analyzed the interaction of host-symbiont genes in amino acid biosynthetic pathways.201526000976
glucose concentration alters dissolved oxygen levels in liquid cultures of beauveria bassiana and affects formation and bioefficacy of blastospores.the filamentous fungus beauveria bassiana is an economically important pathogen of numerous arthropod pests and is able to grow in submerged culture as filaments (mycelia) or as budding yeast-like blastospores. in this study, we evaluated the effect of dissolved oxygen and high glucose concentrations on blastospore production by submerged cultures of two isolates of b. bassiana, esalq1432 and gha. results showed that maintaining adequate dissolved oxygen levels coupled with high glucose concentr ...201525947245
prevalence and genetic diversity of endosymbiotic bacteria infecting cassava whiteflies in africa.cassava provides over half of the dietary requirement for more than 200 million poor in africa. in recent years, cassava has been affected by an epidemic of a virus disease called cassava brown streak disease (cbsd) that is spreading in much of eastern and central africa, affecting food security and the economic development of the poor. the viruses that cause cbsd are transmitted by the insect vector whitefly (bemisia tabaci), which have increased to very high numbers in some african countries. ...201525933928
detection of cucurbit chlorotic yellows virus from bemisia tabaci captured on sticky traps using reverse transcription loop-mediated isothermal amplification (rt-lamp) and simple template preparation.cucurbit chlorotic yellows virus (ccyv) of the genus crinivirus within the family closteroviridae is an emerging infectious agent of cucurbits leading to severe disease and significant economic losses. effective detection and identification methods for this virus are urgently required. in this study, a reverse transcription loop-mediated isothermal amplification (rt-lamp) assay was developed to detect ccyv from its vector bemisia tabaci. lamp primer sets to detect ccyv were evaluated for their s ...201525912723
inhibition of α-glucosidase activity by n-deoxynojirimycin analogs in several insect phloem sap feeders.secondary metabolites and synthetic iminosugars that structurally resemble monosaccharides are potent inhibitors of α-glucosidase activity. the enzyme is core in cleaving sucrose in phloem feeding insects and it also plays a crucial role of reducing osmotic stress via the formation of oligosaccharides. inhibition of hydrolysis by iminosugars should result in nutritional deficiencies and/or disruption of normal osmoregulation. deoxynojirimycin (dnj) and 2 n-alkylated analogs [n-butyl dnj (nb-dnj) ...201625900765
genome reduction and potential metabolic complementation of the dual endosymbionts in the whitefly bemisia tabaci.the whitefly bemisia tabaci is an important agricultural pest with global distribution. this phloem-sap feeder harbors a primary symbiont, "candidatus portiera aleyrodidarum", which compensates for the deficient nutritional composition of its food sources, and a variety of secondary symbionts. interestingly, all of these secondary symbionts are found in co-localization with the primary symbiont within the same bacteriocytes, which should favor the evolution of strong interactions between symbion ...201525887812
comparison of the antennal sensilla ultrastructure of two cryptic species in bemisia tabaci.bemisia tabaci is an important agricultural pest with worldwide distribution and host preference. therefore, understanding the biology of this pest is important to devise specific pest control strategies. the antennae of herbivorous insects play an important role in the identification of hosts using plant volatiles. to understand the features of antennae in b. tabaci meam 1(formerly known as biotype 'b') and med (formerly known as biotype 'q'), the morphology and distribution of the antennal sen ...201525822843
elimination of arsenophonus and decrease in the bacterial symbionts diversity by antibiotic treatment leads to increase in fitness of whitefly, bemisia tabaci.bemisia tabaci is an invasive agricultural pest with more than 24 genetic groups harboring different bacterial endosymbionts categorized into obligatory and facultative endosymbionts. arsenophonus is one of the facultative endosymbionts prevalent in b. tabaci of indian sub-continent. not much is known about the functional role of this endosymbiont in its host. some studies have revealed its involvement in virus transmission by b. tabaci, but how it effects the biology of b. tabaci is unknown. in ...201525801610
cloning of a putative extracellular cu/zn superoxide dismutase and functional differences of superoxide dismutases in invasive and indigenous whiteflies.superoxide dismutases (sods) are a group of important antioxidant defense enzymes. in this study, a putative extracellular cu/zn superoxide dismutase (eccuznsod) complementary dna was cloned and characterized from the whitefly, bemisia tabaci. quantitative polymerase chain reaction analysis showed that the expression level of bteccuznsod was more than 10-fold higher in the invasive middle east asia minor 1 (meam1) than in the native asia ii 3 species of the b. tabaci species complex. after expos ...201525759872
characterization of a novel begomovirus associated with yellow mosaic disease of three ornamental species of jatropha grown in india.severe yellow mosaic disease was observed in three ornamental species of jatropha: j. integerrima, j. podagrica and j. multifida grown in gardens at lucknow, india, during a survey in 2013. the causal pathogen was successfully transmitted from diseased to healthy plants of these species by whitefly (bemisia tabaci). the infection of begomovirus was initially detected in naturally infected plant samples by pcr using begomovirus universal primers. the begomovirus was characterized having a monopar ...201525720372
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